MD09G1282600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008033
Physical Location & Seq
Reverse (-)
35985329 .. 35985940
612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1282600.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGCGTCAACAAGTCAACGACCCCTCCTCCTCATTGCCCTCCTCACCCTGTCCCTCTTCGCCATCTCCGCCACCGCATCAAGGCCCTGCAAAACCCTCTTCGTTTCCTCCTACTCCTTCTCCCTCCGCCGTCTCCCCTCCTCCTCTTCCTCCTCCGGCTTCGTCACCGTCGTCACCGAAATCGGCCAGCTCCGCCCCGTCCACATCCACCCTGCCCGCTCAGAAGACGTCTTCGTTCCACACCACCACAACCACCACCACGACAGCGAGAAGCGGACTCAGACGGCACCGCTCTTTCCCTTCATCGGAACGGCTTCTTCGTCGTCGTCCTACGACCTCAGCTCCCTGCGCGATCGCACCAAGGACATCATGAGCGTCGTCGTTTCGCTGCTTTTCGGCGTTGGCTGCGGCGCGCTCACCGCCGCCACTATGTACCTGGCTTGGTCCGTCTTCATCAGTCGCCACGACGACAGCGGCTCCAGCTCTTTCGATGACGATTTCAAAGACTTCAGCCCGAAGAAGGTGGGTTATGTCAAGATTCCGGAGGTGACCGCTGACTCCGTTTCCCCACCACAGTCTACAGTCAAGGAAGCCGCCGCCGCCGCAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

21.88

Weight (kDa)

7.88

Isoelectric Point (pI)

64.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 231
AccB1I GGYRCC 1 cut(s) 286
AccBSI CCGCTC 2 cut(s) 219, 292
AccI GTMKAC 1 cut(s) 578
AccII CGCG 2 cut(s) 351, 413
AccIII TCCGGA 1 cut(s) 541
AcoI YGGCCR 1 cut(s) 184
AcuI CTGAAG 1 cut(s) 493
AcyI GRCGYC 2 cut(s) 5, 228
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 3 cut(s) 81, 305, 520
AgsI TTSAA 1 cut(s) 502
AjnI CCWGG 1 cut(s) 435
AluBI AGCT 3 cut(s) 190, 342, 483
AluI AGCT 3 cut(s) 190, 342, 483
Alw26I GTCTC 1 cut(s) 137
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 2 cut(s) 83, 184
ApeKI GCWGC 2 cut(s) 388, 405
AsiSI GCGATCGC 1 cut(s) 355
Asp700I GAANNNNTTC 1 cut(s) 313
AspLEI GCGC 3 cut(s) 351, 413, 415
AspS9I GGNCC 2 cut(s) 84, 444
AsuHPI GGTGA 5 cut(s) 37, 157, 166, 409, 559
AvaII GGWCC 1 cut(s) 444
BanI GGYRCC 1 cut(s) 286
BbsI GAAGAC 3 cut(s) 223, 231, 442
BbvCI CCTCAGC 1 cut(s) 338
BbvI GCAGC 2 cut(s) 375, 392
BccI CCATC 1 cut(s) 71
BceAI ACGGC 3 cut(s) 114, 300, 327
BciT130I CCWGG 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 579
BisI GCNGC 9 cut(s) 389, 406, 409, 423, 475, 594, 597, 600, 603
BlsI GCNGC 9 cut(s) 390, 407, 410, 424, 476, 595, 598, 601, 604
Bme1390I CCNGG 1 cut(s) 437
Bme18I GGWCC 1 cut(s) 444
BmgT120I GGNCC 2 cut(s) 84, 444
BmiI GGNNCC 2 cut(s) 288, 478
BmrFI CCNGG 1 cut(s) 437
BmsI GCATC 1 cut(s) 86
BpiI GAAGAC 3 cut(s) 223, 231, 442
BpmI CTGGAG 1 cut(s) 463
Bpu10I CCTNAGC 1 cut(s) 338
BsaHI GRCGYC 2 cut(s) 5, 228
BsaJI CCNNGG 1 cut(s) 360
BsaWI WCCGGW 1 cut(s) 541
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 305, 520
Bse3DI GCAATG 1 cut(s) 33
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 1 cut(s) 360
BseGI GGATG 1 cut(s) 204
BseLI CCNNNNNNNGG 3 cut(s) 81, 305, 520
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 3 cut(s) 234, 293, 352
BsePI GCGCGC 1 cut(s) 411
BseRI GAGGAG 6 cut(s) 17, 20, 32, 130, 133, 142
BseXI GCAGC 2 cut(s) 375, 392
Bsh1236I CGCG 2 cut(s) 351, 413
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 2 cut(s) 85, 186
BshNI GGYRCC 1 cut(s) 286
BsiEI CGRYCG 1 cut(s) 355
BsiSI CCGG 2 cut(s) 156, 542
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 3 cut(s) 81, 305, 520
BsmAI GTCTC 1 cut(s) 137
BsmBI CGTCTC 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 37
BsnI GGCC 2 cut(s) 85, 186
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 1 cut(s) 352
BspANI GGCC 2 cut(s) 85, 186
BspCNI CTCAG 3 cut(s) 233, 292, 351
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 2 cut(s) 351, 413
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 2 cut(s) 288, 478
BspT107I GGYRCC 1 cut(s) 286
BsrBI CCGCTC 2 cut(s) 219, 292
BsrDI GCAATG 1 cut(s) 33
BssECI CCNNGG 1 cut(s) 360
BssHII GCGCGC 1 cut(s) 411
BssMI GATC 1 cut(s) 352
BssNI GRCGYC 2 cut(s) 5, 228
BssT1I CCWWGG 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 3 cut(s) 169, 576, 583
Bst6I CTCTTC 3 cut(s) 62, 104, 151
BstACI GRCGYC 2 cut(s) 5, 228
BstC8I GCNNGC 3 cut(s) 188, 217, 413
BstDEI CTNAG 3 cut(s) 220, 279, 338
BstEII GGTNACC 1 cut(s) 547
BstF5I GGATG 1 cut(s) 204
BstFNI CGCG 2 cut(s) 351, 413
BstHHI GCGC 3 cut(s) 351, 413, 415
BstKTI GATC 1 cut(s) 355
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 1 cut(s) 352
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 8 cut(s) 68, 192, 348, 405, 419, 480, 599, 602
BstNI CCWGG 1 cut(s) 437
BstPI GGTNACC 1 cut(s) 547
BstSCI CCNGG 1 cut(s) 435
BstSFI CTRYAG 1 cut(s) 579
BstUI CGCG 2 cut(s) 351, 413
BstV1I GCAGC 2 cut(s) 375, 392
BstV2I GAAGAC 3 cut(s) 223, 231, 442
BsuRI GGCC 2 cut(s) 85, 186
BtsCI GGATG 1 cut(s) 204
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 1 cut(s) 612
Cac8I GCNNGC 3 cut(s) 188, 217, 413
CciI TCATGA 1 cut(s) 369
CfoI GCGC 3 cut(s) 351, 413, 415
Cfr13I GGNCC 2 cut(s) 84, 444
CseI GACGC 1 cut(s) 364
Csp6I GTAC 1 cut(s) 433
CviAII CATG 1 cut(s) 370
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 3 cut(s) 220, 279, 338
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 3 cut(s) 62, 104, 151
EarI CTCTTC 3 cut(s) 62, 104, 151
EciI GGCGGA 3 cut(s) 58, 116, 182
Eco130I CCWWGG 1 cut(s) 360
Eco47I GGWCC 1 cut(s) 444
Eco57I CTGAAG 1 cut(s) 493
Eco91I GGTNACC 1 cut(s) 547
EcoO109I RGGNCCY 1 cut(s) 84
EcoO65I GGTNACC 1 cut(s) 547
EcoRII CCWGG 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 360
ErhI CCWWGG 1 cut(s) 360
Esp3I CGTCTC 1 cut(s) 137
FaeI CATG 1 cut(s) 373
FaiI YATR 3 cut(s) 371, 431, 531
FaqI GGGAC 1 cut(s) 37
FatI CATG 1 cut(s) 369
FauI CCCGC 1 cut(s) 224
FblI GTMKAC 1 cut(s) 578
Fnu4HI GCNGC 9 cut(s) 389, 406, 409, 423, 475, 594, 597, 600, 603
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 9 cut(s) 389, 406, 409, 423, 475, 594, 597, 600, 603
GlaI GCGC 3 cut(s) 350, 412, 414
GluI GCNGC 9 cut(s) 389, 406, 409, 423, 475, 594, 597, 600, 603
GsuI CTGGAG 1 cut(s) 463
HaeIII GGCC 2 cut(s) 85, 186
HapII CCGG 2 cut(s) 156, 542
HgaI GACGC 1 cut(s) 364
HhaI GCGC 3 cut(s) 351, 413, 415
Hin1I GRCGYC 2 cut(s) 5, 228
Hin1II CATG 1 cut(s) 373
Hin6I GCGC 3 cut(s) 349, 411, 413
HinP1I GCGC 3 cut(s) 349, 411, 413
HincII GTYRAC 2 cut(s) 9, 17
HindII GTYRAC 2 cut(s) 9, 17
HinfI GANTC 3 cut(s) 277, 538, 557
HpaII CCGG 2 cut(s) 156, 542
HphI GGTGA 5 cut(s) 37, 157, 166, 409, 559
Hpy166II GTNNAC 4 cut(s) 9, 17, 202, 579
Hpy188I TCNGA 3 cut(s) 223, 282, 308
Hpy188III TCNNGA 3 cut(s) 370, 535, 542
Hpy8I GTNNAC 4 cut(s) 9, 17, 202, 579
Hpy99I CGWCG 6 cut(s) 173, 325, 328, 380, 383, 470
HpyAV CCTTC 3 cut(s) 127, 310, 514
HpyCH4III ACNGT 3 cut(s) 169, 576, 583
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 1 cut(s) 90
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 192, 348, 405, 419, 480, 599, 602
HpyF3I CTNAG 3 cut(s) 220, 279, 338
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 2 cut(s) 5, 228
Hsp92II CATG 1 cut(s) 373
HspAI GCGC 3 cut(s) 349, 411, 413
Kpn2I TCCGGA 1 cut(s) 541
Kzo9I GATC 1 cut(s) 352
LmnI GCTCC 3 cut(s) 195, 347, 482
Lsp1109I GCAGC 2 cut(s) 375, 392
LweI GCATC 1 cut(s) 86
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 3 cut(s) 163, 172, 547
MalI GATC 1 cut(s) 354
MbiI CCGCTC 2 cut(s) 219, 292
MboI GATC 1 cut(s) 352
MboII GAAGA 8 cut(s) 49, 91, 138, 223, 236, 309, 442, 529
MlyI GAGTC 2 cut(s) 271, 551
MroI TCCGGA 1 cut(s) 541
MroXI GAANNNNTTC 1 cut(s) 313
MspA1I CMGCKG 2 cut(s) 474, 554
MspI CCGG 2 cut(s) 156, 542
MspR9I CCNGG 1 cut(s) 437
MvaI CCWGG 1 cut(s) 437
MvnI CGCG 2 cut(s) 351, 413
MwoI GCNNNNNNNGC 8 cut(s) 68, 192, 348, 405, 419, 480, 599, 602
NdeII GATC 1 cut(s) 352
NlaIII CATG 1 cut(s) 373
NlaIV GGNNCC 2 cut(s) 288, 478
NmuCI GTSAC 3 cut(s) 163, 172, 547
PagI TCATGA 1 cut(s) 369
PauI GCGCGC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 317
PdmI GAANNNNTTC 1 cut(s) 313
PfeI GAWTC 1 cut(s) 538
PkrI GCNGC 9 cut(s) 390, 407, 410, 424, 476, 595, 598, 601, 604
Ple19I CGATCG 1 cut(s) 355
PleI GAGTC 2 cut(s) 271, 551
PpsI GAGTC 2 cut(s) 271, 551
Psp6I CCWGG 1 cut(s) 435
PspEI GGTNACC 1 cut(s) 547
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 2 cut(s) 288, 478
PspPI GGNCC 2 cut(s) 84, 444
PteI GCGCGC 1 cut(s) 411
PvuI CGATCG 1 cut(s) 355
RgaI GCGATCGC 1 cut(s) 355
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
SatI GCNGC 9 cut(s) 389, 406, 409, 423, 475, 594, 597, 600, 603
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 2 cut(s) 84, 444
SchI GAGTC 2 cut(s) 271, 551
ScrFI CCNGG 1 cut(s) 437
SetI ASST 8 cut(s) 192, 231, 339, 344, 438, 485, 525, 549
SfaAI GCGATCGC 1 cut(s) 355
SfaNI GCATC 1 cut(s) 86
SfcI CTRYAG 1 cut(s) 579
SgfI GCGATCGC 1 cut(s) 355
SinI GGWCC 1 cut(s) 444
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 3 cut(s) 169, 576, 583
TaiI ACGT 1 cut(s) 231
TaqI TCGA 1 cut(s) 489
TauI GCSGC 7 cut(s) 411, 425, 477, 596, 599, 602, 605
TfiI GAWTC 1 cut(s) 538
TscAI CASTG 1 cut(s) 612
TseFI GTSAC 3 cut(s) 163, 172, 547
TseI GCWGC 2 cut(s) 388, 405
Tsp45I GTSAC 3 cut(s) 163, 172, 547
TspDTI ATGAA 2 cut(s) 292, 442
TspGWI ACGGA 2 cut(s) 436, 550
TspRI CASTG 1 cut(s) 612
VpaK11BI GGWCC 1 cut(s) 444
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 313
ZraI GACGTC 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.