Rh3AG284000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0008033
Physical Location & Seq
Forward (+)
33258688 .. 33259551
864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG284000.1

Sequence Viewer

Length: 546 bp
ATGGCGTCAACGAGTCAACGACCCCTCCTGCTAGCTCTGCTGATCCTCTCGATCTTGGCAATCTCCGCTACCGCCAGGCCCTGCAAGTCCCTCTTCATCTCCTCCTACTCCGTCTCCCTCAGGCGCTTCCCCTCCTCTTCCTCCTCCTCGGGATTCGTCACCGTCGTCACCGAGATCAGACAACTCAAGCCCATCCGCTTCGACGACGCCGTTTTGGACAACCCCAACCAAAACCAGCCCGACGCCTCCGCCGATGAGGCCCGCCAGCCGCAGCAGAGGATGATGCCTCTCCTCCCCCTCTCCACCGACCTCACCTCCCTCCGCGACCGCACCAAGGACATCCTCAGCGTCGTCGTCGCCCTCCTCTTCGGCGTCGGCTGCGGCGCCCTCACCGCCGCCACCATGTACTTGGCCTGGTCCCTCTTCTCCACCCGCCACGACGACGACGCCGATGACGTCAGCCCTAAGAAGGTGGGGTATGTCAAGATTCCGGAGGCGGTTGTTGACTCAGCACCGGTGAAAGAAGCCAAAGCCGCCGCGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

19.49

Weight (kDa)

7.76

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 459
AccB1I GGYRCC 1 cut(s) 383
AccII CGCG 2 cut(s) 324, 539
AccIII TCCGGA 1 cut(s) 490
AclWI GGATC 1 cut(s) 37
AcyI GRCGYC 7 cut(s) 5, 207, 243, 372, 384, 447, 456
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 2 cut(s) 334, 469
AgeI ACCGGT 1 cut(s) 514
AjnI CCWGG 2 cut(s) 74, 413
AluBI AGCT 1 cut(s) 35
AluI AGCT 1 cut(s) 35
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 37
AlwNI CAGNNNCTG 1 cut(s) 81
Ama87I CYCGRG 1 cut(s) 148
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 3 cut(s) 77, 258, 411
ApeKI GCWGC 2 cut(s) 271, 378
AsiGI ACCGGT 1 cut(s) 514
AspLEI GCGC 2 cut(s) 126, 386
AspS9I GGNCC 3 cut(s) 78, 259, 417
AsuHPI GGTGA 5 cut(s) 151, 160, 304, 382, 529
AsuNHI GCTAGC 1 cut(s) 31
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 417
AxyI CCTNAGG 1 cut(s) 119
BanI GGYRCC 1 cut(s) 383
BbvCI CCTCAGC 1 cut(s) 344
BbvI GCAGC 2 cut(s) 283, 365
BccI CCATC 1 cut(s) 200
BceAI ACGGC 1 cut(s) 194
BciT130I CCWGG 2 cut(s) 76, 415
BcoDI GTCTC 1 cut(s) 118
BfaI CTAG 1 cut(s) 32
BfoI RGCGCY 2 cut(s) 127, 387
BglI GCCNNNNNGGC 1 cut(s) 257
BisI GCNGC 7 cut(s) 269, 272, 379, 382, 396, 534, 537
BlsI GCNGC 7 cut(s) 270, 273, 380, 383, 397, 535, 538
Bme1390I CCNGG 2 cut(s) 76, 415
Bme18I GGWCC 1 cut(s) 417
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 3 cut(s) 78, 259, 417
BmiI GGNNCC 2 cut(s) 385, 419
BmrFI CCNGG 2 cut(s) 76, 415
BmsI GCATC 1 cut(s) 273
BmtI GCTAGC 1 cut(s) 35
Bpu10I CCTNAGC 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 170
BsaHI GRCGYC 7 cut(s) 5, 207, 243, 372, 384, 447, 456
BsaJI CCNNGG 3 cut(s) 147, 333, 537
BsaWI WCCGGW 2 cut(s) 490, 514
BsaXI ACNNNNNCTCC 6 cut(s) 9, 39, 98, 128, 299, 329
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 469
Bse118I RCCGGY 1 cut(s) 514
Bse21I CCTNAGG 1 cut(s) 119
BseAI TCCGGA 1 cut(s) 490
BseBI CCWGG 2 cut(s) 76, 415
BseDI CCNNGG 3 cut(s) 147, 333, 537
BseGI GGATG 3 cut(s) 192, 285, 339
BseLI CCNNNNNNNGG 2 cut(s) 334, 469
BseMII CTCAG 3 cut(s) 133, 358, 522
BseRI GAGGAG 6 cut(s) 91, 124, 133, 136, 281, 353
BseXI GCAGC 2 cut(s) 283, 365
Bsh1236I CGCG 2 cut(s) 324, 539
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 3 cut(s) 79, 260, 413
BshNI GGYRCC 1 cut(s) 383
BshTI ACCGGT 1 cut(s) 514
BsiEI CGRYCG 1 cut(s) 328
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 2 cut(s) 491, 515
BslFI GGGAC 2 cut(s) 73, 403
BslI CCNNNNNNNGG 2 cut(s) 334, 469
BsmAI GTCTC 1 cut(s) 118
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 2 cut(s) 73, 403
BsnI GGCC 3 cut(s) 79, 260, 413
BsoBI CYCGRG 1 cut(s) 148
Bsp13I TCCGGA 1 cut(s) 490
Bsp143I GATC 3 cut(s) 42, 51, 174
BspANI GGCC 3 cut(s) 79, 260, 413
BspCNI CTCAG 3 cut(s) 132, 357, 521
BspEI TCCGGA 1 cut(s) 490
BspFNI CGCG 2 cut(s) 324, 539
BspLI GGNNCC 2 cut(s) 385, 419
BspOI GCTAGC 1 cut(s) 35
BspPI GGATC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 383
BsrFI RCCGGY 1 cut(s) 514
BssAI RCCGGY 1 cut(s) 514
BssECI CCNNGG 3 cut(s) 147, 333, 537
BssMI GATC 3 cut(s) 42, 51, 174
BssNI GRCGYC 7 cut(s) 5, 207, 243, 372, 384, 447, 456
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 2 cut(s) 76, 415
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 4 cut(s) 98, 142, 371, 428
BstACI GRCGYC 7 cut(s) 5, 207, 243, 372, 384, 447, 456
BstC8I GCNNGC 3 cut(s) 33, 262, 266
BstDEI CTNAG 4 cut(s) 119, 344, 465, 508
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 3 cut(s) 192, 285, 339
BstFNI CGCG 2 cut(s) 324, 539
BstH2I RGCGCY 2 cut(s) 127, 387
BstHHI GCGC 2 cut(s) 126, 386
BstKTI GATC 3 cut(s) 45, 54, 177
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 3 cut(s) 42, 51, 174
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 6 cut(s) 37, 65, 257, 378, 392, 533
BstNI CCWGG 2 cut(s) 76, 415
BstSCI CCNGG 2 cut(s) 74, 413
BstUI CGCG 2 cut(s) 324, 539
BstV1I GCAGC 2 cut(s) 283, 365
BstXI CCANNNNNNTGG 1 cut(s) 409
Bsu36I CCTNAGG 1 cut(s) 119
BsuRI GGCC 3 cut(s) 79, 260, 413
BtgI CCRYGG 1 cut(s) 537
BtsCI GGATG 3 cut(s) 192, 285, 339
Cac8I GCNNGC 3 cut(s) 33, 262, 266
CaiI CAGNNNCTG 1 cut(s) 81
CfoI GCGC 2 cut(s) 126, 386
Cfr10I RCCGGY 1 cut(s) 514
Cfr13I GGNCC 3 cut(s) 78, 259, 417
Cfr42I CCGCGG 1 cut(s) 540
CseI GACGC 5 cut(s) 215, 251, 337, 361, 455
Csp6I GTAC 1 cut(s) 406
CspAI ACCGGT 1 cut(s) 514
CviAII CATG 1 cut(s) 403
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 4 cut(s) 119, 344, 465, 508
DinI GGCGCC 1 cut(s) 385
DpnI GATC 3 cut(s) 44, 53, 176
DpnII GATC 3 cut(s) 42, 51, 174
Eam1104I CTCTTC 4 cut(s) 98, 142, 371, 428
EarI CTCTTC 4 cut(s) 98, 142, 371, 428
EciI GGCGGA 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 417
Eco81I CCTNAGG 1 cut(s) 119
Eco88I CYCGRG 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 78
EcoRII CCWGG 2 cut(s) 74, 413
EcoT14I CCWWGG 1 cut(s) 333
EgeI GGCGCC 1 cut(s) 385
EheI GGCGCC 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 333
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 1 cut(s) 406
FaiI YATR 3 cut(s) 404, 480, 544
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 2 cut(s) 73, 403
FatI CATG 1 cut(s) 402
FauI CCCGC 2 cut(s) 269, 440
Fnu4HI GCNGC 7 cut(s) 269, 272, 379, 382, 396, 534, 537
FokI GGATG 3 cut(s) 179, 292, 326
Fsp4HI GCNGC 7 cut(s) 269, 272, 379, 382, 396, 534, 537
FspBI CTAG 1 cut(s) 32
GlaI GCGC 2 cut(s) 125, 385
GluI GCNGC 7 cut(s) 269, 272, 379, 382, 396, 534, 537
HaeII RGCGCY 2 cut(s) 127, 387
HaeIII GGCC 3 cut(s) 79, 260, 413
HapII CCGG 2 cut(s) 491, 515
HgaI GACGC 5 cut(s) 215, 251, 337, 361, 455
HhaI GCGC 2 cut(s) 126, 386
Hin1I GRCGYC 7 cut(s) 5, 207, 243, 372, 384, 447, 456
Hin1II CATG 1 cut(s) 406
Hin6I GCGC 2 cut(s) 124, 384
HinP1I GCGC 2 cut(s) 124, 384
HincII GTYRAC 3 cut(s) 9, 17, 505
HindII GTYRAC 3 cut(s) 9, 17, 505
HinfI GANTC 4 cut(s) 13, 153, 487, 506
HpaII CCGG 2 cut(s) 491, 515
HphI GGTGA 5 cut(s) 151, 160, 304, 382, 529
Hpy166II GTNNAC 3 cut(s) 9, 17, 505
Hpy188I TCNGA 1 cut(s) 179
Hpy188III TCNNGA 4 cut(s) 49, 150, 484, 491
Hpy8I GTNNAC 3 cut(s) 9, 17, 505
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 6 cut(s) 37, 65, 257, 378, 392, 533
HpyF3I CTNAG 4 cut(s) 119, 344, 465, 508
HpySE526I ACGT 1 cut(s) 456
Hsp92I GRCGYC 7 cut(s) 5, 207, 243, 372, 384, 447, 456
Hsp92II CATG 1 cut(s) 406
HspAI GCGC 2 cut(s) 124, 384
KasI GGCGCC 1 cut(s) 383
Kpn2I TCCGGA 1 cut(s) 490
KspI CCGCGG 1 cut(s) 540
Kzo9I GATC 3 cut(s) 42, 51, 174
Lsp1109I GCAGC 2 cut(s) 283, 365
LweI GCATC 1 cut(s) 273
MaeI CTAG 1 cut(s) 32
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 2 cut(s) 157, 166
MalI GATC 3 cut(s) 44, 53, 176
MboI GATC 3 cut(s) 42, 51, 174
MboII GAAGA 4 cut(s) 85, 129, 358, 415
Mly113I GGCGCC 1 cut(s) 384
MlyI GAGTC 2 cut(s) 22, 500
MroI TCCGGA 1 cut(s) 490
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 2 cut(s) 491, 515
MspR9I CCNGG 2 cut(s) 76, 415
MvaI CCWGG 2 cut(s) 76, 415
MvnI CGCG 2 cut(s) 324, 539
MwoI GCNNNNNNNGC 6 cut(s) 37, 65, 257, 378, 392, 533
NarI GGCGCC 1 cut(s) 384
NdeII GATC 3 cut(s) 42, 51, 174
NheI GCTAGC 1 cut(s) 31
NlaIII CATG 1 cut(s) 406
NlaIV GGNNCC 2 cut(s) 385, 419
NmuCI GTSAC 2 cut(s) 157, 166
PcsI WCGNNNNNNNCGW 5 cut(s) 162, 207, 249, 447, 453
PfeI GAWTC 2 cut(s) 153, 487
PinAI ACCGGT 1 cut(s) 514
PkrI GCNGC 7 cut(s) 270, 273, 380, 383, 397, 535, 538
PleI GAGTC 2 cut(s) 21, 500
PluTI GGCGCC 1 cut(s) 387
PpsI GAGTC 2 cut(s) 21, 500
Psp6I CCWGG 2 cut(s) 74, 413
PspGI CCWGG 2 cut(s) 74, 413
PspN4I GGNNCC 2 cut(s) 385, 419
PspPI GGNCC 3 cut(s) 78, 259, 417
PstNI CAGNNNCTG 1 cut(s) 81
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
SacII CCGCGG 1 cut(s) 540
SatI GCNGC 7 cut(s) 269, 272, 379, 382, 396, 534, 537
Sau3AI GATC 3 cut(s) 42, 51, 174
Sau96I GGNCC 3 cut(s) 78, 259, 417
SchI GAGTC 2 cut(s) 22, 500
ScrFI CCNGG 2 cut(s) 76, 415
SetI ASST 5 cut(s) 37, 312, 317, 459, 474
SfaNI GCATC 1 cut(s) 273
SfoI GGCGCC 1 cut(s) 385
Sfr303I CCGCGG 1 cut(s) 540
SgrAI CRCCGGYG 1 cut(s) 514
SgrBI CCGCGG 1 cut(s) 540
SinI GGWCC 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
SspDI GGCGCC 1 cut(s) 383
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 74, 413
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 1 cut(s) 459
TaqI TCGA 2 cut(s) 50, 201
TatI WGTACW 1 cut(s) 405
TauI GCSGC 5 cut(s) 271, 384, 398, 536, 539
TfiI GAWTC 2 cut(s) 153, 487
TseFI GTSAC 2 cut(s) 157, 166
TseI GCWGC 2 cut(s) 271, 378
Tsp45I GTSAC 2 cut(s) 157, 166
TspDTI ATGAA 1 cut(s) 85
TspGWI ACGGA 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 417
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XspI CTAG 1 cut(s) 32
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.