Rw3G025180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0008033
Physical Location & Seq
Forward (+)
33245023 .. 33247831
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025180.1

Sequence Viewer

Length: 564 bp
ATGGCGTCAACGAGTCAACGACCCCTCCTGATAGCTCTGCTGATCCTCTCGATCTTGGCAATCTCCGCCACCGCCAGGCCCTGCAAGTCCCTCTTCATCTCCTCCTACTCCGTCTCCCTCAGGCGCTTCCCCTCCTCTTCCTCCTCCTCGGGATTCGTCACCGTCGTCACCGAGATCAGACAACTCAAGCCCATCCGCTTCGACGACGCCGTTTTGGACAATCGCAACCAAAACCACCCCGACGACGCCGCCGATGAGGCCCGCCAGCCGCAGCAGAGGATGATGCCTCTCCTCCCCCTCTCCACCGACCTCACCTCCCTCCGCGACCGCACCAAGGACATCCTCAGCGTCGTCGTCGCCCTCCTCTTCGGCGTCGGCTGCGGCGCCCTCACCGCCGCCACCATGTACTTGGCCTGGTCCCTCTTCTCCACCCGCCACGACGACGACGACGCCGGTGACGTCAGCCCTAAGAAGGTGGGGTATGTCAAGATTCCGGAGGCGGTTGTTGACTCAGCACCGGTGAAAGAAGCCAAAGCCGCCGCGACTTTCTTAGCTGAATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.3

Weight (kDa)

6.73

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 462
AccB1I GGYRCC 1 cut(s) 383
AccII CGCG 2 cut(s) 324, 542
AccIII TCCGGA 1 cut(s) 493
AclWI GGATC 1 cut(s) 37
AcyI GRCGYC 7 cut(s) 5, 207, 246, 372, 384, 450, 459
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 3 cut(s) 75, 334, 472
AgeI ACCGGT 1 cut(s) 517
AjnI CCWGG 2 cut(s) 74, 413
AluBI AGCT 2 cut(s) 35, 554
AluI AGCT 2 cut(s) 35, 554
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 37
AlwNI CAGNNNCTG 1 cut(s) 81
Ama87I CYCGRG 1 cut(s) 148
Aor13HI TCCGGA 1 cut(s) 493
AoxI GGCC 3 cut(s) 77, 258, 411
ApeKI GCWGC 2 cut(s) 271, 378
AsiGI ACCGGT 1 cut(s) 517
AspLEI GCGC 2 cut(s) 126, 386
AspS9I GGNCC 3 cut(s) 78, 259, 417
AsuHPI GGTGA 6 cut(s) 151, 160, 304, 382, 467, 532
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 417
AxyI CCTNAGG 1 cut(s) 119
BanI GGYRCC 1 cut(s) 383
BbvCI CCTCAGC 1 cut(s) 344
BbvI GCAGC 2 cut(s) 283, 365
BccI CCATC 1 cut(s) 200
BceAI ACGGC 1 cut(s) 194
BciT130I CCWGG 2 cut(s) 76, 415
BcoDI GTCTC 1 cut(s) 118
BfoI RGCGCY 2 cut(s) 127, 387
BglI GCCNNNNNGGC 1 cut(s) 257
BisI GCNGC 8 cut(s) 249, 269, 272, 379, 382, 396, 537, 540
BlsI GCNGC 8 cut(s) 250, 270, 273, 380, 383, 397, 538, 541
Bme1390I CCNGG 2 cut(s) 76, 415
Bme18I GGWCC 1 cut(s) 417
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 3 cut(s) 78, 259, 417
BmiI GGNNCC 2 cut(s) 385, 419
BmrFI CCNGG 2 cut(s) 76, 415
BmsI GCATC 1 cut(s) 273
Bpu10I CCTNAGC 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 170
BsaHI GRCGYC 7 cut(s) 5, 207, 246, 372, 384, 450, 459
BsaJI CCNNGG 2 cut(s) 147, 333
BsaWI WCCGGW 2 cut(s) 493, 517
BsaXI ACNNNNNCTCC 6 cut(s) 9, 39, 98, 128, 299, 329
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 334, 472
Bse118I RCCGGY 2 cut(s) 452, 517
Bse21I CCTNAGG 1 cut(s) 119
BseAI TCCGGA 1 cut(s) 493
BseBI CCWGG 2 cut(s) 76, 415
BseDI CCNNGG 2 cut(s) 147, 333
BseGI GGATG 3 cut(s) 192, 285, 339
BseLI CCNNNNNNNGG 3 cut(s) 75, 334, 472
BseMII CTCAG 3 cut(s) 133, 358, 525
BseRI GAGGAG 6 cut(s) 91, 124, 133, 136, 281, 353
BseXI GCAGC 2 cut(s) 283, 365
Bsh1236I CGCG 2 cut(s) 324, 542
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 3 cut(s) 79, 260, 413
BshNI GGYRCC 1 cut(s) 383
BshTI ACCGGT 1 cut(s) 517
BsiEI CGRYCG 1 cut(s) 328
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 3 cut(s) 453, 494, 518
BslFI GGGAC 2 cut(s) 73, 403
BslI CCNNNNNNNGG 3 cut(s) 75, 334, 472
BsmAI GTCTC 1 cut(s) 118
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 2 cut(s) 73, 403
BsnI GGCC 3 cut(s) 79, 260, 413
BsoBI CYCGRG 1 cut(s) 148
Bsp13I TCCGGA 1 cut(s) 493
Bsp143I GATC 3 cut(s) 42, 51, 174
BspANI GGCC 3 cut(s) 79, 260, 413
BspCNI CTCAG 3 cut(s) 132, 357, 524
BspEI TCCGGA 1 cut(s) 493
BspFNI CGCG 2 cut(s) 324, 542
BspLI GGNNCC 2 cut(s) 385, 419
BspPI GGATC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 383
BsrFI RCCGGY 2 cut(s) 452, 517
BssAI RCCGGY 2 cut(s) 452, 517
BssECI CCNNGG 2 cut(s) 147, 333
BssMI GATC 3 cut(s) 42, 51, 174
BssNI GRCGYC 7 cut(s) 5, 207, 246, 372, 384, 450, 459
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 2 cut(s) 76, 415
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 4 cut(s) 98, 142, 371, 428
BstACI GRCGYC 7 cut(s) 5, 207, 246, 372, 384, 450, 459
BstC8I GCNNGC 2 cut(s) 262, 266
BstDEI CTNAG 5 cut(s) 119, 344, 468, 511, 550
BstF5I GGATG 3 cut(s) 192, 285, 339
BstFNI CGCG 2 cut(s) 324, 542
BstH2I RGCGCY 2 cut(s) 127, 387
BstHHI GCGC 2 cut(s) 126, 386
BstKTI GATC 3 cut(s) 45, 54, 177
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 3 cut(s) 42, 51, 174
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 5 cut(s) 65, 257, 378, 392, 536
BstNI CCWGG 2 cut(s) 76, 415
BstSCI CCNGG 2 cut(s) 74, 413
BstUI CGCG 2 cut(s) 324, 542
BstV1I GCAGC 2 cut(s) 283, 365
BstXI CCANNNNNNTGG 1 cut(s) 409
Bsu36I CCTNAGG 1 cut(s) 119
BsuRI GGCC 3 cut(s) 79, 260, 413
BtsCI GGATG 3 cut(s) 192, 285, 339
Cac8I GCNNGC 2 cut(s) 262, 266
CaiI CAGNNNCTG 1 cut(s) 81
CfoI GCGC 2 cut(s) 126, 386
Cfr10I RCCGGY 2 cut(s) 452, 517
Cfr13I GGNCC 3 cut(s) 78, 259, 417
CseI GACGC 5 cut(s) 215, 254, 337, 361, 458
Csp6I GTAC 1 cut(s) 406
CspAI ACCGGT 1 cut(s) 517
CviAII CATG 1 cut(s) 403
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 5 cut(s) 119, 344, 468, 511, 550
DinI GGCGCC 1 cut(s) 385
DpnI GATC 3 cut(s) 44, 53, 176
DpnII GATC 3 cut(s) 42, 51, 174
Eam1104I CTCTTC 4 cut(s) 98, 142, 371, 428
EarI CTCTTC 4 cut(s) 98, 142, 371, 428
EciI GGCGGA 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 417
Eco81I CCTNAGG 1 cut(s) 119
Eco88I CYCGRG 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 78
EcoRII CCWGG 2 cut(s) 74, 413
EcoT14I CCWWGG 1 cut(s) 333
EgeI GGCGCC 1 cut(s) 385
EheI GGCGCC 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 333
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 1 cut(s) 406
FaiI YATR 2 cut(s) 404, 483
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 2 cut(s) 73, 403
FatI CATG 1 cut(s) 402
FauI CCCGC 2 cut(s) 269, 440
Fnu4HI GCNGC 8 cut(s) 249, 269, 272, 379, 382, 396, 537, 540
FokI GGATG 3 cut(s) 179, 292, 326
Fsp4HI GCNGC 8 cut(s) 249, 269, 272, 379, 382, 396, 537, 540
GlaI GCGC 2 cut(s) 125, 385
GluI GCNGC 8 cut(s) 249, 269, 272, 379, 382, 396, 537, 540
HaeII RGCGCY 2 cut(s) 127, 387
HaeIII GGCC 3 cut(s) 79, 260, 413
HapII CCGG 3 cut(s) 453, 494, 518
HgaI GACGC 5 cut(s) 215, 254, 337, 361, 458
HhaI GCGC 2 cut(s) 126, 386
Hin1I GRCGYC 7 cut(s) 5, 207, 246, 372, 384, 450, 459
Hin1II CATG 1 cut(s) 406
Hin6I GCGC 2 cut(s) 124, 384
HinP1I GCGC 2 cut(s) 124, 384
HincII GTYRAC 3 cut(s) 9, 17, 508
HindII GTYRAC 3 cut(s) 9, 17, 508
HinfI GANTC 4 cut(s) 13, 153, 490, 509
HpaII CCGG 3 cut(s) 453, 494, 518
HphI GGTGA 6 cut(s) 151, 160, 304, 382, 467, 532
Hpy166II GTNNAC 3 cut(s) 9, 17, 508
Hpy188I TCNGA 1 cut(s) 179
Hpy188III TCNNGA 5 cut(s) 28, 49, 150, 487, 494
Hpy8I GTNNAC 3 cut(s) 9, 17, 508
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 257, 378, 392, 536
HpyF3I CTNAG 5 cut(s) 119, 344, 468, 511, 550
HpySE526I ACGT 1 cut(s) 459
Hsp92I GRCGYC 7 cut(s) 5, 207, 246, 372, 384, 450, 459
Hsp92II CATG 1 cut(s) 406
HspAI GCGC 2 cut(s) 124, 384
KasI GGCGCC 1 cut(s) 383
Kpn2I TCCGGA 1 cut(s) 493
Kzo9I GATC 3 cut(s) 42, 51, 174
Lsp1109I GCAGC 2 cut(s) 283, 365
LweI GCATC 1 cut(s) 273
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 3 cut(s) 157, 166, 455
MalI GATC 3 cut(s) 44, 53, 176
MboI GATC 3 cut(s) 42, 51, 174
MboII GAAGA 4 cut(s) 85, 129, 358, 415
Mly113I GGCGCC 1 cut(s) 384
MlyI GAGTC 2 cut(s) 22, 503
MroI TCCGGA 1 cut(s) 493
MspI CCGG 3 cut(s) 453, 494, 518
MspR9I CCNGG 2 cut(s) 76, 415
MvaI CCWGG 2 cut(s) 76, 415
MvnI CGCG 2 cut(s) 324, 542
MwoI GCNNNNNNNGC 5 cut(s) 65, 257, 378, 392, 536
NarI GGCGCC 1 cut(s) 384
NdeII GATC 3 cut(s) 42, 51, 174
NlaIII CATG 1 cut(s) 406
NlaIV GGNNCC 2 cut(s) 385, 419
NmuCI GTSAC 3 cut(s) 157, 166, 455
PcsI WCGNNNNNNNCGW 5 cut(s) 162, 207, 249, 444, 456
PfeI GAWTC 2 cut(s) 153, 490
PinAI ACCGGT 1 cut(s) 517
PkrI GCNGC 8 cut(s) 250, 270, 273, 380, 383, 397, 538, 541
PleI GAGTC 2 cut(s) 21, 503
PluTI GGCGCC 1 cut(s) 387
PpsI GAGTC 2 cut(s) 21, 503
Psp6I CCWGG 2 cut(s) 74, 413
PspGI CCWGG 2 cut(s) 74, 413
PspN4I GGNNCC 2 cut(s) 385, 419
PspPI GGNCC 3 cut(s) 78, 259, 417
PstNI CAGNNNCTG 1 cut(s) 81
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
SatI GCNGC 8 cut(s) 249, 269, 272, 379, 382, 396, 537, 540
Sau3AI GATC 3 cut(s) 42, 51, 174
Sau96I GGNCC 3 cut(s) 78, 259, 417
SchI GAGTC 2 cut(s) 22, 503
ScrFI CCNGG 2 cut(s) 76, 415
SetI ASST 6 cut(s) 37, 312, 317, 462, 477, 556
SfaNI GCATC 1 cut(s) 273
SfoI GGCGCC 1 cut(s) 385
SgrAI CRCCGGYG 2 cut(s) 452, 517
SinI GGWCC 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
SspDI GGCGCC 1 cut(s) 383
StyD4I CCNGG 2 cut(s) 74, 413
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 1 cut(s) 462
TaqI TCGA 2 cut(s) 50, 201
TatI WGTACW 1 cut(s) 405
TauI GCSGC 6 cut(s) 251, 271, 384, 398, 539, 542
TfiI GAWTC 2 cut(s) 153, 490
TseFI GTSAC 3 cut(s) 157, 166, 455
TseI GCWGC 2 cut(s) 271, 378
Tsp45I GTSAC 3 cut(s) 157, 166, 455
TspDTI ATGAA 1 cut(s) 85
TspGWI ACGGA 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 417
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
ZraI GACGTC 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.