Rroxscaffold_6G00394590

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008033
Physical Location & Seq
Reverse (-)
15358408 .. 15361572
3165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394590.1

Sequence Viewer

Length: 714 bp
ATGGATTATCTTCACGATAACAGGAATGTATGTATAACTAAGCAACTAATGGCATGGATATCTCCAAATCCTAAAGTACTAGCATGGATATCTCCAAATCCTACAGTACTAGCATGGATATCTCCATATCCTATGAAAGAGAGAAAGAGAGGCTCTGTGTTTTTCCTCAATCCAATCCATGGCGTCAACGAGTCAACGACCCCTCGTGCTAGCTCTGCTGATCCTCTCGATCTTGGCAATCTCCGCCACCGCCAGGCCCTGCAAGTCCCTCTTCATCGCCTCCTACTCCGTCTCCCTCAGGCGCCTCCCCCCTCCTCTTCCTCCTCCTCGGGATTCGTCACCGTCGTCACCGAGATCAGACAACTCAAGCCCATCCGCTTCGACGACGCCGTTTTGGTCCAGCCCGACGACTCCGCCGATGAGGCCCGCCAGCGGCAGCAGAGGATGATGCCTCTCCTCCCTCTCTCCACCGACCTCACCTCCCTCCGCGAGCGCACCAAGGACATCCTCAGCGTCGTCGTCGCCCTCCTCTTCGGCGTCGGCTGCGGCGCCCTCACCGCCGCCACCATGTACTTGGCCTGGTCCCTCTTCTCCACCCGCCACGACGACGACGACGCCGATGACGTCAGCCCTAAGAAGGTGGGGTATGTCAAGATTCCGGAGGCGGTTGTTGACTCAGCACCGGTGAAAGCAGCCAAAGCCGCCGCGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

237

Amino Acids

26.11

Weight (kDa)

8.9

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 627
AccB1I GGYRCC 2 cut(s) 301, 548
AccB7I CCANNNNNTGG 1 cut(s) 179
AccII CGCG 2 cut(s) 489, 707
AccIII TCCGGA 1 cut(s) 658
AclWI GGATC 1 cut(s) 215
AcyI GRCGYC 7 cut(s) 183, 302, 387, 537, 549, 615, 624
AfaI GTAC 3 cut(s) 78, 108, 572
AfiI CCNNNNNNNGG 4 cut(s) 179, 253, 432, 637
AgeI ACCGGT 1 cut(s) 682
AjnI CCWGG 2 cut(s) 252, 578
AluBI AGCT 1 cut(s) 213
AluI AGCT 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 1 cut(s) 215
AlwNI CAGNNNCTG 1 cut(s) 259
Ama87I CYCGRG 1 cut(s) 328
Aor13HI TCCGGA 1 cut(s) 658
AoxI GGCC 3 cut(s) 255, 423, 576
ApeKI GCWGC 3 cut(s) 436, 543, 692
AsiGI ACCGGT 1 cut(s) 682
AspLEI GCGC 3 cut(s) 304, 495, 551
AspS9I GGNCC 4 cut(s) 256, 397, 424, 582
AsuHPI GGTGA 5 cut(s) 331, 340, 469, 547, 697
AsuNHI GCTAGC 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 328
AvaII GGWCC 2 cut(s) 397, 582
AxyI CCTNAGG 1 cut(s) 297
BanI GGYRCC 2 cut(s) 301, 548
BauI CACGAG 1 cut(s) 204
BbvCI CCTCAGC 1 cut(s) 509
BbvI GCAGC 3 cut(s) 448, 530, 704
BccI CCATC 1 cut(s) 380
BceAI ACGGC 1 cut(s) 374
BciT130I CCWGG 2 cut(s) 254, 580
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 3 cut(s) 80, 110, 210
BfmI CTRYAG 1 cut(s) 102
BfoI RGCGCY 2 cut(s) 305, 552
BglI GCCNNNNNGGC 1 cut(s) 422
BisI GCNGC 8 cut(s) 434, 437, 544, 547, 561, 693, 702, 705
BlsI GCNGC 8 cut(s) 435, 438, 545, 548, 562, 694, 703, 706
BmcAI AGTACT 2 cut(s) 78, 108
Bme1390I CCNGG 2 cut(s) 254, 580
Bme18I GGWCC 2 cut(s) 397, 582
BmeT110I CYCGRG 1 cut(s) 328
BmgT120I GGNCC 4 cut(s) 256, 397, 424, 582
BmiI GGNNCC 3 cut(s) 303, 550, 584
BmrFI CCNGG 2 cut(s) 254, 580
BmsI GCATC 1 cut(s) 438
BmtI GCTAGC 1 cut(s) 213
Bpu10I CCTNAGC 1 cut(s) 509
BpuEI CTTGAG 1 cut(s) 350
BsaHI GRCGYC 7 cut(s) 183, 302, 387, 537, 549, 615, 624
BsaJI CCNNGG 4 cut(s) 178, 327, 498, 705
BsaWI WCCGGW 2 cut(s) 658, 682
BsaXI ACNNNNNCTCC 4 cut(s) 276, 306, 464, 494
Bsc4I CCNNNNNNNGG 4 cut(s) 179, 253, 432, 637
Bse118I RCCGGY 1 cut(s) 682
Bse21I CCTNAGG 1 cut(s) 297
BseAI TCCGGA 1 cut(s) 658
BseBI CCWGG 2 cut(s) 254, 580
BseDI CCNNGG 4 cut(s) 178, 327, 498, 705
BseGI GGATG 3 cut(s) 372, 450, 504
BseLI CCNNNNNNNGG 4 cut(s) 179, 253, 432, 637
BseMII CTCAG 3 cut(s) 311, 523, 690
BseRI GAGGAG 5 cut(s) 304, 313, 316, 446, 518
BseXI GCAGC 3 cut(s) 448, 530, 704
Bsh1236I CGCG 2 cut(s) 489, 707
BshFI GGCC 3 cut(s) 257, 425, 578
BshNI GGYRCC 2 cut(s) 301, 548
BshTI ACCGGT 1 cut(s) 682
BsiHKCI CYCGRG 1 cut(s) 328
BsiSI CCGG 2 cut(s) 659, 683
BslFI GGGAC 2 cut(s) 251, 568
BslI CCNNNNNNNGG 4 cut(s) 179, 253, 432, 637
BsmAI GTCTC 1 cut(s) 296
BsmBI CGTCTC 1 cut(s) 296
BsmFI GGGAC 2 cut(s) 251, 568
BsnI GGCC 3 cut(s) 257, 425, 578
BsoBI CYCGRG 1 cut(s) 328
Bsp13I TCCGGA 1 cut(s) 658
Bsp143I GATC 3 cut(s) 220, 229, 354
Bsp19I CCATGG 1 cut(s) 178
BspANI GGCC 3 cut(s) 257, 425, 578
BspCNI CTCAG 3 cut(s) 310, 522, 689
BspEI TCCGGA 1 cut(s) 658
BspFNI CGCG 2 cut(s) 489, 707
BspLI GGNNCC 3 cut(s) 303, 550, 584
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 1 cut(s) 215
BspT107I GGYRCC 2 cut(s) 301, 548
BsrFI RCCGGY 1 cut(s) 682
BssAI RCCGGY 1 cut(s) 682
BssECI CCNNGG 4 cut(s) 178, 327, 498, 705
BssMI GATC 3 cut(s) 220, 229, 354
BssNI GRCGYC 7 cut(s) 183, 302, 387, 537, 549, 615, 624
BssSI CACGAG 1 cut(s) 204
BssT1I CCWWGG 2 cut(s) 178, 498
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 2 cut(s) 254, 580
Bst4CI ACNGT 2 cut(s) 106, 343
Bst6I CTCTTC 4 cut(s) 276, 322, 536, 593
BstACI GRCGYC 7 cut(s) 183, 302, 387, 537, 549, 615, 624
BstC8I GCNNGC 4 cut(s) 211, 427, 431, 491
BstDEI CTNAG 5 cut(s) 39, 297, 509, 633, 676
BstDSI CCRYGG 2 cut(s) 178, 705
BstF5I GGATG 3 cut(s) 372, 450, 504
BstFNI CGCG 2 cut(s) 489, 707
BstH2I RGCGCY 2 cut(s) 305, 552
BstHHI GCGC 3 cut(s) 304, 495, 551
BstKTI GATC 3 cut(s) 223, 232, 357
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 3 cut(s) 220, 229, 354
BstMWI GCNNNNNNNGC 7 cut(s) 215, 243, 422, 543, 557, 698, 701
BstNI CCWGG 2 cut(s) 254, 580
BstSCI CCNGG 2 cut(s) 252, 578
BstSFI CTRYAG 1 cut(s) 102
BstUI CGCG 2 cut(s) 489, 707
BstV1I GCAGC 3 cut(s) 448, 530, 704
BstXI CCANNNNNNTGG 1 cut(s) 574
Bsu36I CCTNAGG 1 cut(s) 297
BsuRI GGCC 3 cut(s) 257, 425, 578
BtgI CCRYGG 2 cut(s) 178, 705
BtgZI GCGATG 1 cut(s) 260
BtsCI GGATG 3 cut(s) 372, 450, 504
Cac8I GCNNGC 4 cut(s) 211, 427, 431, 491
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 3 cut(s) 304, 495, 551
Cfr10I RCCGGY 1 cut(s) 682
Cfr13I GGNCC 4 cut(s) 256, 397, 424, 582
Cfr42I CCGCGG 1 cut(s) 708
CseI GACGC 5 cut(s) 172, 395, 502, 526, 623
Csp6I GTAC 3 cut(s) 77, 107, 571
CspAI ACCGGT 1 cut(s) 682
CviAII CATG 5 cut(s) 54, 84, 114, 179, 568
CviQI GTAC 3 cut(s) 77, 107, 571
DdeI CTNAG 5 cut(s) 39, 297, 509, 633, 676
DinI GGCGCC 2 cut(s) 303, 550
DpnI GATC 3 cut(s) 222, 231, 356
DpnII GATC 3 cut(s) 220, 229, 354
Eam1104I CTCTTC 4 cut(s) 276, 322, 536, 593
EarI CTCTTC 4 cut(s) 276, 322, 536, 593
EciI GGCGGA 2 cut(s) 233, 403
Eco130I CCWWGG 2 cut(s) 178, 498
Eco32I GATATC 3 cut(s) 60, 90, 120
Eco47I GGWCC 2 cut(s) 397, 582
Eco81I CCTNAGG 1 cut(s) 297
Eco88I CYCGRG 1 cut(s) 328
EcoO109I RGGNCCY 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 252, 578
EcoRV GATATC 3 cut(s) 60, 90, 120
EcoT14I CCWWGG 2 cut(s) 178, 498
EgeI GGCGCC 2 cut(s) 303, 550
EheI GGCGCC 2 cut(s) 303, 550
ErhI CCWWGG 2 cut(s) 178, 498
Esp3I CGTCTC 1 cut(s) 296
FaeI CATG 5 cut(s) 57, 87, 117, 182, 571
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FaqI GGGAC 2 cut(s) 251, 568
FatI CATG 5 cut(s) 53, 83, 113, 178, 567
FauI CCCGC 2 cut(s) 434, 605
Fnu4HI GCNGC 8 cut(s) 434, 437, 544, 547, 561, 693, 702, 705
FokI GGATG 3 cut(s) 359, 457, 491
Fsp4HI GCNGC 8 cut(s) 434, 437, 544, 547, 561, 693, 702, 705
FspBI CTAG 3 cut(s) 80, 110, 210
GlaI GCGC 3 cut(s) 303, 494, 550
GluI GCNGC 8 cut(s) 434, 437, 544, 547, 561, 693, 702, 705
HaeII RGCGCY 2 cut(s) 305, 552
HaeIII GGCC 3 cut(s) 257, 425, 578
HapII CCGG 2 cut(s) 659, 683
HgaI GACGC 5 cut(s) 172, 395, 502, 526, 623
HhaI GCGC 3 cut(s) 304, 495, 551
Hin1I GRCGYC 7 cut(s) 183, 302, 387, 537, 549, 615, 624
Hin1II CATG 5 cut(s) 57, 87, 117, 182, 571
Hin6I GCGC 3 cut(s) 302, 493, 549
HinP1I GCGC 3 cut(s) 302, 493, 549
HincII GTYRAC 3 cut(s) 187, 195, 673
HindII GTYRAC 3 cut(s) 187, 195, 673
HinfI GANTC 5 cut(s) 191, 333, 410, 655, 674
HpaII CCGG 2 cut(s) 659, 683
HphI GGTGA 5 cut(s) 331, 340, 469, 547, 697
Hpy166II GTNNAC 3 cut(s) 187, 195, 673
Hpy188I TCNGA 1 cut(s) 359
Hpy188III TCNNGA 5 cut(s) 14, 227, 330, 652, 659
Hpy8I GTNNAC 3 cut(s) 187, 195, 673
HpyAV CCTTC 1 cut(s) 631
HpyCH4III ACNGT 2 cut(s) 106, 343
HpyCH4IV ACGT 1 cut(s) 624
HpyCH4V TGCA 1 cut(s) 262
HpyF10VI GCNNNNNNNGC 7 cut(s) 215, 243, 422, 543, 557, 698, 701
HpyF3I CTNAG 5 cut(s) 39, 297, 509, 633, 676
HpySE526I ACGT 1 cut(s) 624
Hsp92I GRCGYC 7 cut(s) 183, 302, 387, 537, 549, 615, 624
Hsp92II CATG 5 cut(s) 57, 87, 117, 182, 571
HspAI GCGC 3 cut(s) 302, 493, 549
KasI GGCGCC 2 cut(s) 301, 548
Kpn2I TCCGGA 1 cut(s) 658
KspI CCGCGG 1 cut(s) 708
Kzo9I GATC 3 cut(s) 220, 229, 354
Lsp1109I GCAGC 3 cut(s) 448, 530, 704
LweI GCATC 1 cut(s) 438
MaeI CTAG 3 cut(s) 80, 110, 210
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 2 cut(s) 337, 346
MalI GATC 3 cut(s) 222, 231, 356
MboI GATC 3 cut(s) 220, 229, 354
MboII GAAGA 4 cut(s) 263, 309, 523, 580
Mly113I GGCGCC 2 cut(s) 302, 549
MlyI GAGTC 3 cut(s) 200, 404, 668
MroI TCCGGA 1 cut(s) 658
MspA1I CMGCKG 2 cut(s) 433, 707
MspI CCGG 2 cut(s) 659, 683
MspR9I CCNGG 2 cut(s) 254, 580
MvaI CCWGG 2 cut(s) 254, 580
MvnI CGCG 2 cut(s) 489, 707
MwoI GCNNNNNNNGC 7 cut(s) 215, 243, 422, 543, 557, 698, 701
NarI GGCGCC 2 cut(s) 302, 549
NcoI CCATGG 1 cut(s) 178
NdeII GATC 3 cut(s) 220, 229, 354
NheI GCTAGC 1 cut(s) 209
NlaIII CATG 5 cut(s) 57, 87, 117, 182, 571
NlaIV GGNNCC 3 cut(s) 303, 550, 584
NmuCI GTSAC 2 cut(s) 337, 346
PcsI WCGNNNNNNNCGW 6 cut(s) 342, 387, 414, 609, 615, 621
PfeI GAWTC 2 cut(s) 333, 655
PflMI CCANNNNNTGG 1 cut(s) 179
PinAI ACCGGT 1 cut(s) 682
PkrI GCNGC 8 cut(s) 435, 438, 545, 548, 562, 694, 703, 706
PleI GAGTC 3 cut(s) 199, 404, 668
PluTI GGCGCC 2 cut(s) 305, 552
PpsI GAGTC 3 cut(s) 199, 404, 668
Psp6I CCWGG 2 cut(s) 252, 578
PspGI CCWGG 2 cut(s) 252, 578
PspN4I GGNNCC 3 cut(s) 303, 550, 584
PspPI GGNCC 4 cut(s) 256, 397, 424, 582
PstNI CAGNNNCTG 1 cut(s) 259
RsaI GTAC 3 cut(s) 78, 108, 572
RsaNI GTAC 3 cut(s) 77, 107, 571
SacII CCGCGG 1 cut(s) 708
SatI GCNGC 8 cut(s) 434, 437, 544, 547, 561, 693, 702, 705
Sau3AI GATC 3 cut(s) 220, 229, 354
Sau96I GGNCC 4 cut(s) 256, 397, 424, 582
ScaI AGTACT 2 cut(s) 78, 108
SchI GAGTC 3 cut(s) 200, 404, 668
ScrFI CCNGG 2 cut(s) 254, 580
SetI ASST 5 cut(s) 215, 477, 482, 627, 642
SfaNI GCATC 1 cut(s) 438
SfcI CTRYAG 1 cut(s) 102
SfoI GGCGCC 2 cut(s) 303, 550
Sfr303I CCGCGG 1 cut(s) 708
SgrAI CRCCGGYG 1 cut(s) 682
SgrBI CCGCGG 1 cut(s) 708
SinI GGWCC 2 cut(s) 397, 582
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SspDI GGCGCC 2 cut(s) 301, 548
SspMI CTAG 3 cut(s) 80, 110, 210
StyD4I CCNGG 2 cut(s) 252, 578
StyI CCWWGG 2 cut(s) 178, 498
TaaI ACNGT 2 cut(s) 106, 343
TaiI ACGT 1 cut(s) 627
TaqI TCGA 2 cut(s) 228, 381
TatI WGTACW 3 cut(s) 76, 106, 570
TauI GCSGC 5 cut(s) 436, 549, 563, 704, 707
TfiI GAWTC 2 cut(s) 333, 655
TseFI GTSAC 2 cut(s) 337, 346
TseI GCWGC 3 cut(s) 436, 543, 692
Tsp45I GTSAC 2 cut(s) 337, 346
TspDTI ATGAA 2 cut(s) 149, 263
TspGWI ACGGA 1 cut(s) 278
Van91I CCANNNNNTGG 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 397, 582
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XspI CTAG 3 cut(s) 80, 110, 210
ZraI GACGTC 1 cut(s) 625
ZrmI AGTACT 2 cut(s) 78, 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.