RLG00000022985

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008033
Physical Location & Seq
Reverse (-)
16256363 .. 16257644
1282 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022985

Sequence Viewer

Length: 564 bp
ATGGCGTCAACGAGTCAACGACCCCTCTTGCTAGCTCTGCTGATCCTCTCGATCTTGGCAATCTCCGCCACCGCCAGGCCCTGCAAGTCCCTCTTCATCGCCTCCTACTCCGTCTCCCTCAGGCGCCTCCCCTCCTCTTCCTCCTCCTCGGGATTCGTCACCGTCGTCACCGAGATCAGACAACTCAAGCCCATCCGCTTCGACGACGCCGTTTTTGTCCAGCCCGACGACGCCGCCGCCTCCGCCGATGAGGCCCGCCAGCCGCAGCAGAGGACGATGCCTCTCCTCCCTCTCTCCACCGACCTCACCTCCCTCCGCGACCGCACCAAGGACATCCTCAGCGTCGTCGTCGCCCTCCTCTTCGGCGTCGGCTGCGGGGCCCTCACCGCCGCCACCATGTACTTGGCCTGGTCCCTCTTCTCCACCCGCCACGACGACGACGATGCCGATGATGTCAGCCCTAAGAAGGTGGGGTATGTCAAGATTACGGAGGCGGTTGTTGACTCAGCACCGGTGAAAGAAGCCAAAGCCGCCGCGGTTTTTCTTTTTATCTCGAGTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

19.99

Weight (kDa)

6.57

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 123
AccII CGCG 2 cut(s) 318, 536
AclWI GGATC 1 cut(s) 37
AcyI GRCGYC 5 cut(s) 5, 124, 207, 231, 366
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 3 cut(s) 75, 328, 466
AgeI ACCGGT 1 cut(s) 511
AjnI CCWGG 2 cut(s) 74, 407
AluBI AGCT 1 cut(s) 35
AluI AGCT 1 cut(s) 35
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 37
AlwNI CAGNNNCTG 1 cut(s) 81
Ama87I CYCGRG 2 cut(s) 148, 553
AoxI GGCC 4 cut(s) 77, 252, 378, 405
ApaI GGGCCC 1 cut(s) 382
ApeKI GCWGC 2 cut(s) 265, 372
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AsiGI ACCGGT 1 cut(s) 511
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 5 cut(s) 78, 253, 378, 379, 411
AsuHPI GGTGA 5 cut(s) 151, 160, 298, 376, 526
AsuNHI GCTAGC 1 cut(s) 31
AvaI CYCGRG 2 cut(s) 148, 553
AvaII GGWCC 1 cut(s) 411
AxyI CCTNAGG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 382
BanI GGYRCC 1 cut(s) 123
BanII GRGCYC 1 cut(s) 382
BbvCI CCTCAGC 1 cut(s) 338
BbvI GCAGC 2 cut(s) 277, 359
BccI CCATC 1 cut(s) 200
BceAI ACGGC 1 cut(s) 194
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 2 cut(s) 76, 409
BcoDI GTCTC 1 cut(s) 118
BfaI CTAG 1 cut(s) 32
BfoI RGCGCY 1 cut(s) 127
BglI GCCNNNNNGGC 1 cut(s) 251
BisI GCNGC 8 cut(s) 234, 237, 263, 266, 373, 390, 531, 534
BlsI GCNGC 8 cut(s) 235, 238, 264, 267, 374, 391, 532, 535
Bme1390I CCNGG 2 cut(s) 76, 409
Bme18I GGWCC 1 cut(s) 411
BmeT110I CYCGRG 2 cut(s) 148, 553
BmgT120I GGNCC 5 cut(s) 78, 253, 378, 379, 411
BmiI GGNNCC 4 cut(s) 125, 379, 380, 413
BmrFI CCNGG 2 cut(s) 76, 409
BmsI GCATC 2 cut(s) 267, 433
BmtI GCTAGC 1 cut(s) 35
Bpu10I CCTNAGC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 170
BsaHI GRCGYC 5 cut(s) 5, 124, 207, 231, 366
BsaJI CCNNGG 3 cut(s) 147, 327, 534
BsaWI WCCGGW 1 cut(s) 511
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 293, 323
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 328, 466
Bse118I RCCGGY 1 cut(s) 511
Bse21I CCTNAGG 1 cut(s) 119
BseBI CCWGG 2 cut(s) 76, 409
BseDI CCNNGG 3 cut(s) 147, 327, 534
BseGI GGATG 2 cut(s) 192, 333
BseLI CCNNNNNNNGG 3 cut(s) 75, 328, 466
BseMII CTCAG 3 cut(s) 133, 352, 519
BseRI GAGGAG 5 cut(s) 124, 133, 136, 275, 347
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 2 cut(s) 277, 359
Bsh1236I CGCG 2 cut(s) 318, 536
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 4 cut(s) 79, 254, 380, 407
BshNI GGYRCC 1 cut(s) 123
BshTI ACCGGT 1 cut(s) 511
BsiEI CGRYCG 1 cut(s) 322
BsiHKCI CYCGRG 2 cut(s) 148, 553
BsiSI CCGG 1 cut(s) 512
BslFI GGGAC 2 cut(s) 73, 397
BslI CCNNNNNNNGG 3 cut(s) 75, 328, 466
BsmAI GTCTC 1 cut(s) 118
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 2 cut(s) 73, 397
BsnI GGCC 4 cut(s) 79, 254, 380, 407
BsoBI CYCGRG 2 cut(s) 148, 553
Bsp120I GGGCCC 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 3 cut(s) 42, 51, 174
BspANI GGCC 4 cut(s) 79, 254, 380, 407
BspCNI CTCAG 3 cut(s) 132, 351, 518
BspFNI CGCG 2 cut(s) 318, 536
BspLI GGNNCC 4 cut(s) 125, 379, 380, 413
BspOI GCTAGC 1 cut(s) 35
BspPI GGATC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 511
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 3 cut(s) 147, 327, 534
BssMI GATC 3 cut(s) 42, 51, 174
BssNI GRCGYC 5 cut(s) 5, 124, 207, 231, 366
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 76, 409
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 4 cut(s) 98, 142, 365, 422
BstACI GRCGYC 5 cut(s) 5, 124, 207, 231, 366
BstC8I GCNNGC 3 cut(s) 33, 256, 260
BstDEI CTNAG 4 cut(s) 119, 338, 462, 505
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 2 cut(s) 192, 333
BstFNI CGCG 2 cut(s) 318, 536
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 3 cut(s) 45, 54, 177
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 3 cut(s) 42, 51, 174
BstMCI CGRYCG 1 cut(s) 322
BstMWI GCNNNNNNNGC 7 cut(s) 37, 65, 242, 251, 372, 386, 530
BstNI CCWGG 2 cut(s) 76, 409
BstSCI CCNGG 2 cut(s) 74, 407
BstSLI GKGCMC 1 cut(s) 382
BstUI CGCG 2 cut(s) 318, 536
BstV1I GCAGC 2 cut(s) 277, 359
BstXI CCANNNNNNTGG 1 cut(s) 403
Bsu36I CCTNAGG 1 cut(s) 119
BsuRI GGCC 4 cut(s) 79, 254, 380, 407
BtgI CCRYGG 1 cut(s) 534
BtgZI GCGATG 1 cut(s) 82
BtsCI GGATG 2 cut(s) 192, 333
Cac8I GCNNGC 3 cut(s) 33, 256, 260
CaiI CAGNNNCTG 1 cut(s) 81
CfoI GCGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 511
Cfr13I GGNCC 5 cut(s) 78, 253, 378, 379, 411
Cfr42I CCGCGG 1 cut(s) 537
CseI GACGC 4 cut(s) 215, 239, 331, 355
Csp6I GTAC 1 cut(s) 400
CspAI ACCGGT 1 cut(s) 511
CviAII CATG 1 cut(s) 397
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 4 cut(s) 119, 338, 462, 505
DinI GGCGCC 1 cut(s) 125
DpnI GATC 3 cut(s) 44, 53, 176
DpnII GATC 3 cut(s) 42, 51, 174
Eam1104I CTCTTC 4 cut(s) 98, 142, 365, 422
EarI CTCTTC 4 cut(s) 98, 142, 365, 422
EciI GGCGGA 2 cut(s) 55, 232
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 411
Eco81I CCTNAGG 1 cut(s) 119
Eco88I CYCGRG 2 cut(s) 148, 553
EcoO109I RGGNCCY 3 cut(s) 78, 378, 379
EcoRII CCWGG 2 cut(s) 74, 407
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 1 cut(s) 382
EgeI GGCGCC 1 cut(s) 125
EheI GGCGCC 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 327
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 1 cut(s) 400
FaiI YATR 2 cut(s) 398, 477
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 2 cut(s) 73, 397
FatI CATG 1 cut(s) 396
FauI CCCGC 3 cut(s) 263, 368, 434
Fnu4HI GCNGC 8 cut(s) 234, 237, 263, 266, 373, 390, 531, 534
FokI GGATG 2 cut(s) 179, 320
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 8 cut(s) 234, 237, 263, 266, 373, 390, 531, 534
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 125
GluI GCNGC 8 cut(s) 234, 237, 263, 266, 373, 390, 531, 534
HaeII RGCGCY 1 cut(s) 127
HaeIII GGCC 4 cut(s) 79, 254, 380, 407
HapII CCGG 1 cut(s) 512
HgaI GACGC 4 cut(s) 215, 239, 331, 355
HhaI GCGC 1 cut(s) 126
Hin1I GRCGYC 5 cut(s) 5, 124, 207, 231, 366
Hin1II CATG 1 cut(s) 400
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HincII GTYRAC 3 cut(s) 9, 17, 502
HindII GTYRAC 3 cut(s) 9, 17, 502
HinfI GANTC 3 cut(s) 13, 153, 503
HpaII CCGG 1 cut(s) 512
HphI GGTGA 5 cut(s) 151, 160, 298, 376, 526
Hpy166II GTNNAC 3 cut(s) 9, 17, 502
Hpy188I TCNGA 1 cut(s) 179
Hpy188III TCNNGA 4 cut(s) 49, 150, 481, 553
Hpy8I GTNNAC 3 cut(s) 9, 17, 502
HpyAV CCTTC 1 cut(s) 460
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4V TGCA 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 65, 242, 251, 372, 386, 530
HpyF3I CTNAG 4 cut(s) 119, 338, 462, 505
Hsp92I GRCGYC 5 cut(s) 5, 124, 207, 231, 366
Hsp92II CATG 1 cut(s) 400
HspAI GCGC 1 cut(s) 124
KasI GGCGCC 1 cut(s) 123
KspI CCGCGG 1 cut(s) 537
Kzo9I GATC 3 cut(s) 42, 51, 174
LpnPI CCDG 9 cut(s) 61, 88, 94, 106, 233, 272, 394, 421, 525
Lsp1109I GCAGC 2 cut(s) 277, 359
LweI GCATC 2 cut(s) 267, 433
MaeI CTAG 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 157, 166
MalI GATC 3 cut(s) 44, 53, 176
MboI GATC 3 cut(s) 42, 51, 174
MboII GAAGA 4 cut(s) 85, 129, 352, 409
MhlI GDGCHC 1 cut(s) 382
Mly113I GGCGCC 1 cut(s) 124
MlyI GAGTC 2 cut(s) 22, 497
MspA1I CMGCKG 1 cut(s) 536
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 2 cut(s) 76, 409
MvaI CCWGG 2 cut(s) 76, 409
MvnI CGCG 2 cut(s) 318, 536
MwoI GCNNNNNNNGC 7 cut(s) 37, 65, 242, 251, 372, 386, 530
NarI GGCGCC 1 cut(s) 124
NdeII GATC 3 cut(s) 42, 51, 174
NheI GCTAGC 1 cut(s) 31
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 4 cut(s) 125, 379, 380, 413
NmuCI GTSAC 2 cut(s) 157, 166
PaeR7I CTCGAG 1 cut(s) 553
PcsI WCGNNNNNNNCGW 4 cut(s) 162, 207, 438, 444
PfeI GAWTC 1 cut(s) 153
PinAI ACCGGT 1 cut(s) 511
PkrI GCNGC 8 cut(s) 235, 238, 264, 267, 374, 391, 532, 535
PleI GAGTC 2 cut(s) 21, 497
PluTI GGCGCC 1 cut(s) 127
PpsI GAGTC 2 cut(s) 21, 497
Psp6I CCWGG 2 cut(s) 74, 407
PspGI CCWGG 2 cut(s) 74, 407
PspN4I GGNNCC 4 cut(s) 125, 379, 380, 413
PspOMI GGGCCC 1 cut(s) 378
PspPI GGNCC 5 cut(s) 78, 253, 378, 379, 411
PstNI CAGNNNCTG 1 cut(s) 81
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
SacII CCGCGG 1 cut(s) 537
SatI GCNGC 8 cut(s) 234, 237, 263, 266, 373, 390, 531, 534
Sau3AI GATC 3 cut(s) 42, 51, 174
Sau96I GGNCC 5 cut(s) 78, 253, 378, 379, 411
SchI GAGTC 2 cut(s) 22, 497
ScrFI CCNGG 2 cut(s) 76, 409
SduI GDGCHC 1 cut(s) 382
SetI ASST 4 cut(s) 37, 306, 311, 471
SfaNI GCATC 2 cut(s) 267, 433
SfoI GGCGCC 1 cut(s) 125
Sfr274I CTCGAG 1 cut(s) 553
Sfr303I CCGCGG 1 cut(s) 537
SgrAI CRCCGGYG 1 cut(s) 511
SgrBI CCGCGG 1 cut(s) 537
SinI GGWCC 1 cut(s) 411
SlaI CTCGAG 1 cut(s) 553
SmlI CTYRAG 2 cut(s) 185, 553
SmoI CTYRAG 2 cut(s) 185, 553
SspDI GGCGCC 1 cut(s) 123
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 74, 407
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 163
TaqI TCGA 3 cut(s) 50, 201, 554
TatI WGTACW 1 cut(s) 399
TauI GCSGC 6 cut(s) 236, 239, 265, 392, 533, 536
TfiI GAWTC 1 cut(s) 153
TseFI GTSAC 2 cut(s) 157, 166
TseI GCWGC 2 cut(s) 265, 372
Tsp45I GTSAC 2 cut(s) 157, 166
TspDTI ATGAA 1 cut(s) 85
TspGWI ACGGA 2 cut(s) 100, 503
VpaK11BI GGWCC 1 cut(s) 411
XcmI CCANNNNNNNNNTGG 1 cut(s) 400
XhoI CTCGAG 1 cut(s) 553
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.