Rh3BG320400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008033
Physical Location & Seq
Forward (+)
32958148 .. 32961639
3492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG320400.1

Sequence Viewer

Length: 537 bp
ATGGCGTCAACGAGTCAACGACCCCTCCTGATAGCTCTGCTGATCCTCTCGATCTTGGCAATCTCCGCCACCGCCAGGCCCTGCAAGACCCTCTTCATCTCCTCCTACTCCGTCTCCCTCAGGCGCCTCCCCTCCTCTTCCTCCTCCTCGGGATTCGTCACCGTCGTCACCGAGATCAGACAACTCAAGCCCATCCGCTTCGACGACGCCGTTATGGTCCAGCCCGACGACGCCGCCGCCGCCGATGAGGCCCGCCAGCCGCAGCAGAGGATGATGCCTCTCCTCCCCCTCTCCACCGACCTCACCTCCCTCCGCGACCGCACCAAGGACATCCTCAGCGTCGTCGTCGCCCTCCTCTTCGGCGTCGGCTGCGGCGCCCTCACCGCCGCCACCATGTACTTGGCCTGGTCCCTCTTCTCCACCCGCCACGACGGCGGCGTCGATGACGTCAGCCCTAAGAAGATGGGGTATGTCAAGATTCCGGAGGCGGTTGTTGACTCAGCACCGGTAAAAGAAGCCAAAGCCGCCGCGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

19.02

Weight (kDa)

9.0

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 437
AatII GACGTC 1 cut(s) 450
AccB1I GGYRCC 2 cut(s) 123, 374
AccII CGCG 2 cut(s) 315, 530
AccIII TCCGGA 1 cut(s) 481
AclWI GGATC 1 cut(s) 37
AcyI GRCGYC 8 cut(s) 5, 124, 207, 231, 363, 375, 438, 447
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 2 cut(s) 75, 325
AgeI ACCGGT 1 cut(s) 505
AjnI CCWGG 2 cut(s) 74, 404
AluBI AGCT 1 cut(s) 35
AluI AGCT 1 cut(s) 35
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 37
AlwNI CAGNNNCTG 1 cut(s) 81
Ama87I CYCGRG 1 cut(s) 148
Aor13HI TCCGGA 1 cut(s) 481
AoxI GGCC 3 cut(s) 77, 249, 402
ApeKI GCWGC 2 cut(s) 262, 369
AsiGI ACCGGT 1 cut(s) 505
AspLEI GCGC 2 cut(s) 126, 377
AspS9I GGNCC 4 cut(s) 78, 217, 250, 408
AsuHPI GGTGA 4 cut(s) 151, 160, 295, 373
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 2 cut(s) 217, 408
AxyI CCTNAGG 1 cut(s) 119
BanI GGYRCC 2 cut(s) 123, 374
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 2 cut(s) 274, 356
BccI CCATC 2 cut(s) 200, 457
BceAI ACGGC 2 cut(s) 194, 448
BciT130I CCWGG 2 cut(s) 76, 406
BcoDI GTCTC 1 cut(s) 118
BfoI RGCGCY 2 cut(s) 127, 378
BglI GCCNNNNNGGC 2 cut(s) 248, 432
Bme1390I CCNGG 2 cut(s) 76, 406
Bme18I GGWCC 2 cut(s) 217, 408
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 4 cut(s) 78, 217, 250, 408
BmiI GGNNCC 3 cut(s) 125, 376, 410
BmrFI CCNGG 2 cut(s) 76, 406
BmsI GCATC 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 170
BsaHI GRCGYC 8 cut(s) 5, 124, 207, 231, 363, 375, 438, 447
BsaJI CCNNGG 3 cut(s) 147, 324, 528
BsaWI WCCGGW 2 cut(s) 481, 505
BsaXI ACNNNNNCTCC 6 cut(s) 9, 39, 98, 128, 290, 320
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 325
Bse118I RCCGGY 1 cut(s) 505
Bse21I CCTNAGG 1 cut(s) 119
BseAI TCCGGA 1 cut(s) 481
BseBI CCWGG 2 cut(s) 76, 406
BseDI CCNNGG 3 cut(s) 147, 324, 528
BseGI GGATG 3 cut(s) 192, 276, 330
BseLI CCNNNNNNNGG 2 cut(s) 75, 325
BseMII CTCAG 3 cut(s) 133, 349, 513
BseRI GAGGAG 6 cut(s) 91, 124, 133, 136, 272, 344
BseXI GCAGC 2 cut(s) 274, 356
Bsh1236I CGCG 2 cut(s) 315, 530
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 3 cut(s) 79, 251, 404
BshNI GGYRCC 2 cut(s) 123, 374
BshTI ACCGGT 1 cut(s) 505
BsiEI CGRYCG 1 cut(s) 319
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 2 cut(s) 482, 506
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 2 cut(s) 75, 325
BsmAI GTCTC 1 cut(s) 118
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 3 cut(s) 79, 251, 404
BsoBI CYCGRG 1 cut(s) 148
Bsp13I TCCGGA 1 cut(s) 481
Bsp143I GATC 3 cut(s) 42, 51, 174
BspANI GGCC 3 cut(s) 79, 251, 404
BspCNI CTCAG 3 cut(s) 132, 348, 512
BspEI TCCGGA 1 cut(s) 481
BspFNI CGCG 2 cut(s) 315, 530
BspLI GGNNCC 3 cut(s) 125, 376, 410
BspPI GGATC 1 cut(s) 37
BspT107I GGYRCC 2 cut(s) 123, 374
BsrFI RCCGGY 1 cut(s) 505
BssAI RCCGGY 1 cut(s) 505
BssECI CCNNGG 3 cut(s) 147, 324, 528
BssMI GATC 3 cut(s) 42, 51, 174
BssNI GRCGYC 8 cut(s) 5, 124, 207, 231, 363, 375, 438, 447
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 2 cut(s) 76, 406
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 4 cut(s) 98, 142, 362, 419
BstACI GRCGYC 8 cut(s) 5, 124, 207, 231, 363, 375, 438, 447
BstC8I GCNNGC 2 cut(s) 253, 257
BstDEI CTNAG 4 cut(s) 119, 335, 456, 499
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 3 cut(s) 192, 276, 330
BstFNI CGCG 2 cut(s) 315, 530
BstH2I RGCGCY 2 cut(s) 127, 378
BstHHI GCGC 2 cut(s) 126, 377
BstKTI GATC 3 cut(s) 45, 54, 177
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 3 cut(s) 42, 51, 174
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 7 cut(s) 65, 239, 248, 369, 383, 432, 524
BstNI CCWGG 2 cut(s) 76, 406
BstSCI CCNGG 2 cut(s) 74, 404
BstUI CGCG 2 cut(s) 315, 530
BstV1I GCAGC 2 cut(s) 274, 356
BstXI CCANNNNNNTGG 1 cut(s) 400
Bsu36I CCTNAGG 1 cut(s) 119
BsuRI GGCC 3 cut(s) 79, 251, 404
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 3 cut(s) 192, 276, 330
Cac8I GCNNGC 2 cut(s) 253, 257
CaiI CAGNNNCTG 1 cut(s) 81
CfoI GCGC 2 cut(s) 126, 377
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 4 cut(s) 78, 217, 250, 408
Cfr42I CCGCGG 1 cut(s) 531
CseI GACGC 5 cut(s) 215, 239, 328, 352, 427
Csp6I GTAC 1 cut(s) 397
CspAI ACCGGT 1 cut(s) 505
CviAII CATG 1 cut(s) 394
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 4 cut(s) 119, 335, 456, 499
DinI GGCGCC 2 cut(s) 125, 376
DpnI GATC 3 cut(s) 44, 53, 176
DpnII GATC 3 cut(s) 42, 51, 174
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
Eam1104I CTCTTC 4 cut(s) 98, 142, 362, 419
EarI CTCTTC 4 cut(s) 98, 142, 362, 419
EciI GGCGGA 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 324
Eco47I GGWCC 2 cut(s) 217, 408
Eco81I CCTNAGG 1 cut(s) 119
Eco88I CYCGRG 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 78
EcoRII CCWGG 2 cut(s) 74, 404
EcoT14I CCWWGG 1 cut(s) 324
EgeI GGCGCC 2 cut(s) 125, 376
EheI GGCGCC 2 cut(s) 125, 376
ErhI CCWWGG 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 1 cut(s) 397
FaiI YATR 4 cut(s) 215, 395, 471, 535
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 1 cut(s) 394
FatI CATG 1 cut(s) 393
FauI CCCGC 2 cut(s) 260, 431
FokI GGATG 3 cut(s) 179, 283, 317
GlaI GCGC 2 cut(s) 125, 376
HaeII RGCGCY 2 cut(s) 127, 378
HaeIII GGCC 3 cut(s) 79, 251, 404
HapII CCGG 2 cut(s) 482, 506
HgaI GACGC 5 cut(s) 215, 239, 328, 352, 427
HhaI GCGC 2 cut(s) 126, 377
Hin1I GRCGYC 8 cut(s) 5, 124, 207, 231, 363, 375, 438, 447
Hin1II CATG 1 cut(s) 397
Hin6I GCGC 2 cut(s) 124, 375
HinP1I GCGC 2 cut(s) 124, 375
HincII GTYRAC 3 cut(s) 9, 17, 496
HindII GTYRAC 3 cut(s) 9, 17, 496
HinfI GANTC 4 cut(s) 13, 153, 478, 497
HpaII CCGG 2 cut(s) 482, 506
HphI GGTGA 4 cut(s) 151, 160, 295, 373
Hpy166II GTNNAC 3 cut(s) 9, 17, 496
Hpy188I TCNGA 1 cut(s) 179
Hpy188III TCNNGA 5 cut(s) 28, 49, 150, 475, 482
Hpy8I GTNNAC 3 cut(s) 9, 17, 496
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 239, 248, 369, 383, 432, 524
HpyF3I CTNAG 4 cut(s) 119, 335, 456, 499
HpySE526I ACGT 1 cut(s) 447
Hsp92I GRCGYC 8 cut(s) 5, 124, 207, 231, 363, 375, 438, 447
Hsp92II CATG 1 cut(s) 397
HspAI GCGC 2 cut(s) 124, 375
KasI GGCGCC 2 cut(s) 123, 374
Kpn2I TCCGGA 1 cut(s) 481
KspI CCGCGG 1 cut(s) 531
Kzo9I GATC 3 cut(s) 42, 51, 174
Lsp1109I GCAGC 2 cut(s) 274, 356
LweI GCATC 1 cut(s) 264
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 2 cut(s) 157, 166
MalI GATC 3 cut(s) 44, 53, 176
MboI GATC 3 cut(s) 42, 51, 174
MboII GAAGA 5 cut(s) 85, 129, 349, 406, 472
Mly113I GGCGCC 2 cut(s) 124, 375
MlyI GAGTC 2 cut(s) 22, 491
MroI TCCGGA 1 cut(s) 481
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 2 cut(s) 482, 506
MspR9I CCNGG 2 cut(s) 76, 406
MvaI CCWGG 2 cut(s) 76, 406
MvnI CGCG 2 cut(s) 315, 530
MwoI GCNNNNNNNGC 7 cut(s) 65, 239, 248, 369, 383, 432, 524
NarI GGCGCC 2 cut(s) 124, 375
NdeII GATC 3 cut(s) 42, 51, 174
NlaIII CATG 1 cut(s) 397
NlaIV GGNNCC 3 cut(s) 125, 376, 410
NmuCI GTSAC 2 cut(s) 157, 166
PcsI WCGNNNNNNNCGW 4 cut(s) 162, 207, 435, 438
PfeI GAWTC 2 cut(s) 153, 478
PinAI ACCGGT 1 cut(s) 505
PleI GAGTC 2 cut(s) 21, 491
PluTI GGCGCC 2 cut(s) 127, 378
PpsI GAGTC 2 cut(s) 21, 491
Psp6I CCWGG 2 cut(s) 74, 404
PspGI CCWGG 2 cut(s) 74, 404
PspN4I GGNNCC 3 cut(s) 125, 376, 410
PspPI GGNCC 4 cut(s) 78, 217, 250, 408
PstNI CAGNNNCTG 1 cut(s) 81
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
SacII CCGCGG 1 cut(s) 531
Sau3AI GATC 3 cut(s) 42, 51, 174
Sau96I GGNCC 4 cut(s) 78, 217, 250, 408
SchI GAGTC 2 cut(s) 22, 491
ScrFI CCNGG 2 cut(s) 76, 406
SetI ASST 4 cut(s) 37, 303, 308, 450
SfaNI GCATC 1 cut(s) 264
SfoI GGCGCC 2 cut(s) 125, 376
Sfr303I CCGCGG 1 cut(s) 531
SgrBI CCGCGG 1 cut(s) 531
SinI GGWCC 2 cut(s) 217, 408
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
SspDI GGCGCC 2 cut(s) 123, 374
StyD4I CCNGG 2 cut(s) 74, 404
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 1 cut(s) 450
TaqI TCGA 3 cut(s) 50, 201, 441
TatI WGTACW 1 cut(s) 396
TauI GCSGC 9 cut(s) 236, 239, 242, 262, 375, 389, 438, 527, 530
TfiI GAWTC 2 cut(s) 153, 478
TseFI GTSAC 2 cut(s) 157, 166
TseI GCWGC 2 cut(s) 262, 369
Tsp45I GTSAC 2 cut(s) 157, 166
TspDTI ATGAA 1 cut(s) 85
TspGWI ACGGA 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 217, 408
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
ZraI GACGTC 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.