MD10G1022100.v1.1

SKP1-interacting partner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006283
Physical Location & Seq
Forward (+)
2789056 .. 2790141
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1022100.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGACGCTCTCCCGCAGGACACGCTCCACCAGATCTTCTCGAGCCTTCCCCTCCGGCAGATCATGATCTGCCGCTCACTCTCCAAGTTCTTCAACCAACTCCTCACCTCACCCTCCTTTGTCCACATCATCTCCACCCAATCTCCGCCCCTCAACCTCCTCGCCCTCCGCCCTCCCCACCACCACCACCAGCGCCACGTGTCCTCCTCATCCTGCCTCCACGTCTTCGACCCCGACCTCAACCAATGGATCAGATTCGCCCTCGATTTCCTCCCCTTCCGCTCACCACACCCCGTCGCCTCCTCTCTCGGCCTCGTCTACCTCTGGGGCGACTCGCCCGACCCGCCCGAGTCAGCCAAGTCCCTCGTCGTCTGTAACCCTCTGACTCGTCAGTTCCGGGTCCTCCCTCAGCTCGGCTCGGCCTGGTCGCGCCACGGCTCGGTCCTCGTTGACTCCCAAACTCGAGTCATGGTCCTCACCGAGCTCGCCGCGCTCTACTTCTCCGGTTCTAACCAGTGGCTCAAGTTCTCATCGAACCTCCCGTCAAAACCCAGAAGCCCAATTTTGGTGTTTGACTCGGTTTACGCCCTCTGCGATGTGGGTTCGCCGTGGAGGAGTGAATGGAAGTTGTTCCACTGCACAATTTCGAAGCTCAAGACCTCGCAGACTTGGACTCGGCTCGAAAAGCACGATTGGGGGGACGTCTTTGACATCCTGAAAAGACCCCGTTTAGTTCGCGGAAATGGGAACAATGTGCTGATGGTTGGTGGGTTGAAGTCGTCTTTCTCGCTGAACGCTTCGTGTTCGACGATTCTGATTCTGAGGCTGGACTTGGATAGCTTGGAGTGGGACGAGGCGGGCCGAATGCCGGTGGAGATGTTCAAGTGCTTTCAAGAGTCGAGCAAGTTCAAGGTGTTTGGTGGCGGCGATAGGGTCTGTTTTTCGGCAAAGAGAATTGGGAGATTGGCTTTGTGGGACCGTTGTTCCGAGAAAGCTGAGTGGCGGTGGATTGATGGGGTGCCCGGGAGCGGCGATGGGCTTTCCCGGGGGTTTGTTTTTGATGCCACGCTCACAGCATTTCCTTGA

Protein Analysis

362

Amino Acids

40.74

Weight (kDa)

9.1

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 3 - 40 9.1e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 705
AccB1I GGYRCC 1 cut(s) 1018
AccBSI CCGCTC 3 cut(s) 75, 282, 1029
AccI GTMKAC 1 cut(s) 318
AccII CGCG 3 cut(s) 430, 491, 738
AclWI GGATC 1 cut(s) 257
AcvI CACGTG 1 cut(s) 199
AcyI GRCGYC 1 cut(s) 702
AfiI CCNNNNNNNGG 5 cut(s) 413, 439, 565, 868, 1028
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 5 cut(s) 94, 775, 883, 893, 910
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 1 cut(s) 422
AluBI AGCT 5 cut(s) 412, 484, 652, 840, 995
AluI AGCT 5 cut(s) 412, 484, 652, 840, 995
Alw21I GWGCWC 1 cut(s) 486
AlwI GGATC 1 cut(s) 257
Ama87I CYCGRG 5 cut(s) 40, 347, 462, 1022, 1044
AoxI GGCC 3 cut(s) 310, 420, 859
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp700I GAANNNNTTC 1 cut(s) 629
AspLEI GCGC 3 cut(s) 195, 432, 493
AspS9I GGNCC 5 cut(s) 400, 442, 472, 859, 976
AsuC2I CCSGG 5 cut(s) 398, 1023, 1024, 1045, 1046
AsuHPI GGTGA 4 cut(s) 97, 102, 276, 469
AsuII TTCGAA 1 cut(s) 647
AvaI CYCGRG 5 cut(s) 40, 347, 462, 1022, 1044
AvaII GGWCC 4 cut(s) 400, 442, 472, 976
BaeGI GKGCMC 1 cut(s) 1023
BanI GGYRCC 1 cut(s) 1018
BanII GRGCYC 1 cut(s) 486
BbrPI CACGTG 1 cut(s) 199
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 486
BbvCI CCTCAGC 1 cut(s) 408
BccI CCATC 3 cut(s) 754, 1007, 1028
BceAI ACGGC 2 cut(s) 451, 592
BciT130I CCWGG 1 cut(s) 424
BcnI CCSGG 5 cut(s) 398, 1023, 1024, 1045, 1046
BfoI RGCGCY 1 cut(s) 196
BglII AGATCT 1 cut(s) 33
BisI GCNGC 4 cut(s) 73, 489, 925, 1030
BlsI GCNGC 4 cut(s) 74, 490, 926, 1031
Bme1390I CCNGG 6 cut(s) 398, 424, 1023, 1024, 1045, 1046
Bme18I GGWCC 4 cut(s) 400, 442, 472, 976
BmeT110I CYCGRG 5 cut(s) 40, 347, 462, 1022, 1044
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 5 cut(s) 400, 442, 472, 859, 976
BmiI GGNNCC 3 cut(s) 401, 977, 1020
BmrFI CCNGG 6 cut(s) 398, 424, 1023, 1024, 1045, 1046
BmsI GCATC 1 cut(s) 1051
BpiI GAAGAC 1 cut(s) 217
Bpu10I CCTNAGC 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 647
BpuEI CTTGAG 2 cut(s) 506, 638
BpuMI CCSGG 5 cut(s) 398, 1023, 1024, 1045, 1046
BsaAI YACGTR 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 702
BsaJI CCNNGG 5 cut(s) 433, 608, 1022, 1044, 1045
BsaWI WCCGGW 1 cut(s) 503
BsaXI ACNNNNNCTCC 6 cut(s) 116, 127, 146, 157, 188, 218
Bsc4I CCNNNNNNNGG 5 cut(s) 413, 439, 565, 868, 1028
Bse118I RCCGGY 1 cut(s) 868
Bse1I ACTGG 1 cut(s) 514
Bse8I GATNNNNATC 1 cut(s) 65
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 5 cut(s) 433, 608, 1022, 1044, 1045
BseGI GGATG 2 cut(s) 209, 711
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 5 cut(s) 413, 439, 565, 868, 1028
BseMII CTCAG 3 cut(s) 422, 812, 987
BseNI ACTGG 1 cut(s) 514
BseRI GAGGAG 5 cut(s) 92, 149, 196, 292, 628
BseSI GKGCMC 1 cut(s) 1023
BsgI GTGCAG 1 cut(s) 622
Bsh1236I CGCG 3 cut(s) 430, 491, 738
BshFI GGCC 3 cut(s) 312, 422, 861
BshNI GGYRCC 1 cut(s) 1018
BsiHKAI GWGCWC 1 cut(s) 486
BsiHKCI CYCGRG 5 cut(s) 40, 347, 462, 1022, 1044
BsiSI CCGG 6 cut(s) 55, 397, 504, 869, 1023, 1045
BslFI GGGAC 4 cut(s) 346, 713, 863, 989
BslI CCNNNNNNNGG 5 cut(s) 413, 439, 565, 868, 1028
BsmFI GGGAC 4 cut(s) 346, 713, 863, 989
BsmI GAATGC 1 cut(s) 870
BsnI GGCC 3 cut(s) 312, 422, 861
BsoBI CYCGRG 5 cut(s) 40, 347, 462, 1022, 1044
Bsp119I TTCGAA 1 cut(s) 647
Bsp1286I GDGCHC 2 cut(s) 486, 1023
Bsp143I GATC 4 cut(s) 33, 60, 66, 249
BspANI GGCC 3 cut(s) 312, 422, 861
BspCNI CTCAG 3 cut(s) 421, 813, 988
BspFNI CGCG 3 cut(s) 430, 491, 738
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 3 cut(s) 401, 977, 1020
BspPI GGATC 1 cut(s) 257
BspT104I TTCGAA 1 cut(s) 647
BspT107I GGYRCC 1 cut(s) 1018
BsrBI CCGCTC 3 cut(s) 75, 282, 1029
BsrFI RCCGGY 1 cut(s) 868
BsrI ACTGG 1 cut(s) 514
BssAI RCCGGY 1 cut(s) 868
BssECI CCNNGG 5 cut(s) 433, 608, 1022, 1044, 1045
BssMI GATC 4 cut(s) 33, 60, 66, 249
BssNI GRCGYC 1 cut(s) 702
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 1 cut(s) 980
BstACI GRCGYC 1 cut(s) 702
BstBAI YACGTR 1 cut(s) 199
BstBI TTCGAA 1 cut(s) 647
BstC8I GCNNGC 2 cut(s) 486, 859
BstDEI CTNAG 3 cut(s) 408, 821, 996
BstDSI CCRYGG 2 cut(s) 433, 608
BstF5I GGATG 2 cut(s) 209, 711
BstFNI CGCG 3 cut(s) 430, 491, 738
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 3 cut(s) 195, 432, 493
BstKTI GATC 4 cut(s) 36, 63, 69, 252
BstMBI GATC 4 cut(s) 33, 60, 66, 249
BstMWI GCNNNNNNNGC 4 cut(s) 22, 343, 490, 685
BstNI CCWGG 1 cut(s) 424
BstSCI CCNGG 6 cut(s) 396, 422, 1021, 1022, 1043, 1044
BstSLI GKGCMC 1 cut(s) 1023
BstUI CGCG 3 cut(s) 430, 491, 738
BstV2I GAAGAC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 3 cut(s) 312, 422, 861
BtgI CCRYGG 2 cut(s) 433, 608
BtgZI GCGATG 2 cut(s) 609, 1047
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 2 cut(s) 209, 711
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 2 cut(s) 521, 634
Cac8I GCNNGC 2 cut(s) 486, 859
CciI TCATGA 1 cut(s) 63
CfoI GCGC 3 cut(s) 195, 432, 493
Cfr10I RCCGGY 1 cut(s) 868
Cfr13I GGNCC 5 cut(s) 400, 442, 472, 859, 976
Cfr9I CCCGGG 2 cut(s) 1022, 1044
CseI GACGC 1 cut(s) 14
CviAII CATG 2 cut(s) 64, 469
DdeI CTNAG 3 cut(s) 408, 821, 996
DpnI GATC 4 cut(s) 35, 62, 68, 251
DpnII GATC 4 cut(s) 33, 60, 66, 249
EciI GGCGGA 2 cut(s) 135, 158
Ecl136II GAGCTC 1 cut(s) 484
Eco24I GRGCYC 1 cut(s) 486
Eco47I GGWCC 4 cut(s) 400, 442, 472, 976
Eco53kI GAGCTC 1 cut(s) 484
Eco72I CACGTG 1 cut(s) 199
Eco88I CYCGRG 5 cut(s) 40, 347, 462, 1022, 1044
EcoICRI GAGCTC 1 cut(s) 484
EcoO109I RGGNCCY 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 422
EcoT38I GRGCYC 1 cut(s) 486
FaeI CATG 2 cut(s) 67, 472
FaiI YATR 2 cut(s) 65, 470
FaqI GGGAC 4 cut(s) 346, 713, 863, 989
FatI CATG 2 cut(s) 63, 468
FauI CCCGC 3 cut(s) 21, 351, 850
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 4 cut(s) 73, 489, 925, 1030
FokI GGATG 2 cut(s) 196, 698
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 4 cut(s) 73, 489, 925, 1030
GlaI GCGC 3 cut(s) 194, 431, 492
GluI GCNGC 4 cut(s) 73, 489, 925, 1030
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 3 cut(s) 312, 422, 861
HapII CCGG 6 cut(s) 55, 397, 504, 869, 1023, 1045
HgaI GACGC 1 cut(s) 14
HhaI GCGC 3 cut(s) 195, 432, 493
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 2 cut(s) 67, 472
Hin6I GCGC 3 cut(s) 193, 430, 491
HinP1I GCGC 3 cut(s) 193, 430, 491
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HpaII CCGG 6 cut(s) 55, 397, 504, 869, 1023, 1045
HphI GGTGA 4 cut(s) 97, 102, 276, 469
Hpy166II GTNNAC 4 cut(s) 124, 319, 451, 583
Hpy188I TCNGA 5 cut(s) 254, 384, 816, 822, 988
Hpy188III TCNNGA 5 cut(s) 40, 64, 655, 715, 893
Hpy8I GTNNAC 4 cut(s) 124, 319, 451, 583
Hpy99I CGWCG 3 cut(s) 299, 371, 811
HpyAV CCTTC 2 cut(s) 56, 286
HpyCH4III ACNGT 1 cut(s) 980
HpyCH4IV ACGT 3 cut(s) 198, 222, 702
HpyCH4V TGCA 1 cut(s) 639
HpyF10VI GCNNNNNNNGC 4 cut(s) 22, 343, 490, 685
HpyF3I CTNAG 3 cut(s) 408, 821, 996
HpySE526I ACGT 3 cut(s) 198, 222, 702
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 2 cut(s) 67, 472
HspAI GCGC 3 cut(s) 193, 430, 491
Kzo9I GATC 4 cut(s) 33, 60, 66, 249
LmnI GCTCC 2 cut(s) 30, 1026
LweI GCATC 1 cut(s) 1051
MaeII ACGT 3 cut(s) 198, 222, 702
MaeIII GTNAC 1 cut(s) 374
MalI GATC 4 cut(s) 35, 62, 68, 251
MbiI CCGCTC 3 cut(s) 75, 282, 1029
MboI GATC 4 cut(s) 33, 60, 66, 249
MboII GAAGA 3 cut(s) 28, 82, 217
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 2 cut(s) 486, 1023
MluCI AATT 3 cut(s) 561, 642, 954
MlyI GAGTC 8 cut(s) 326, 359, 379, 446, 474, 569, 667, 905
MroXI GAANNNNTTC 1 cut(s) 629
MspI CCGG 6 cut(s) 55, 397, 504, 869, 1023, 1045
MspR9I CCNGG 6 cut(s) 398, 424, 1023, 1024, 1045, 1046
Mva1269I GAATGC 1 cut(s) 870
MvaI CCWGG 1 cut(s) 424
MvnI CGCG 3 cut(s) 430, 491, 738
MwoI GCNNNNNNNGC 4 cut(s) 22, 343, 490, 685
NciI CCSGG 5 cut(s) 398, 1023, 1024, 1045, 1046
NdeII GATC 4 cut(s) 33, 60, 66, 249
NlaIII CATG 2 cut(s) 67, 472
NlaIV GGNNCC 3 cut(s) 401, 977, 1020
NmeAIII GCCGAG 4 cut(s) 288, 393, 398, 655
NspV TTCGAA 1 cut(s) 647
PaeR7I CTCGAG 2 cut(s) 40, 462
PagI TCATGA 1 cut(s) 63
PcsI WCGNNNNNNNCGW 4 cut(s) 539, 591, 687, 806
PctI GAATGC 1 cut(s) 870
PdmI GAANNNNTTC 1 cut(s) 629
PfeI GAWTC 3 cut(s) 255, 811, 817
PkrI GCNGC 4 cut(s) 74, 490, 926, 1031
PleI GAGTC 8 cut(s) 326, 358, 379, 446, 473, 569, 667, 904
PmaCI CACGTG 1 cut(s) 199
PmlI CACGTG 1 cut(s) 199
PpsI GAGTC 8 cut(s) 326, 358, 379, 446, 473, 569, 667, 904
Ppu21I YACGTR 1 cut(s) 199
PpuMI RGGWCCY 1 cut(s) 400
Psp124BI GAGCTC 1 cut(s) 486
Psp5II RGGWCCY 1 cut(s) 400
Psp6I CCWGG 1 cut(s) 422
PspCI CACGTG 1 cut(s) 199
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 3 cut(s) 401, 977, 1020
PspPI GGNCC 5 cut(s) 400, 442, 472, 859, 976
PspPPI RGGWCCY 1 cut(s) 400
PspXI VCTCGAGB 1 cut(s) 462
PsuI RGATCY 1 cut(s) 33
SacI GAGCTC 1 cut(s) 486
SatI GCNGC 4 cut(s) 73, 489, 925, 1030
Sau3AI GATC 4 cut(s) 33, 60, 66, 249
Sau96I GGNCC 5 cut(s) 400, 442, 472, 859, 976
SchI GAGTC 8 cut(s) 326, 359, 379, 446, 474, 569, 667, 905
ScrFI CCNGG 6 cut(s) 398, 424, 1023, 1024, 1045, 1046
SduI GDGCHC 2 cut(s) 486, 1023
SfaNI GCATC 1 cut(s) 1051
Sfr274I CTCGAG 2 cut(s) 40, 462
SfuI TTCGAA 1 cut(s) 647
SinI GGWCC 4 cut(s) 400, 442, 472, 976
SlaI CTCGAG 2 cut(s) 40, 462
SmaI CCCGGG 2 cut(s) 1024, 1046
SmlI CTYRAG 4 cut(s) 40, 462, 521, 653
SmoI CTYRAG 4 cut(s) 40, 462, 521, 653
Sse9I AATT 3 cut(s) 561, 642, 954
SstI GAGCTC 1 cut(s) 486
StyD4I CCNGG 6 cut(s) 396, 422, 1021, 1022, 1043, 1044
TaaI ACNGT 1 cut(s) 980
TaiI ACGT 3 cut(s) 201, 225, 705
TaqI TCGA 9 cut(s) 41, 228, 264, 463, 533, 647, 681, 806, 899
TaqII GACCGA 1 cut(s) 430
TasI AATT 3 cut(s) 561, 642, 954
TauI GCSGC 4 cut(s) 75, 491, 927, 1032
TfiI GAWTC 3 cut(s) 255, 811, 817
TscAI CASTG 2 cut(s) 521, 641
TspMI CCCGGG 2 cut(s) 1022, 1044
TspRI CASTG 2 cut(s) 521, 641
VpaK11BI GGWCC 4 cut(s) 400, 442, 472, 976
XhoI CTCGAG 2 cut(s) 40, 462
XmaI CCCGGG 2 cut(s) 1022, 1044
XmiI GTMKAC 1 cut(s) 318
XmnI GAANNNNTTC 1 cut(s) 629
ZraI GACGTC 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.