Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0006283

Physical Location & Seq

Genomic Coordinates Forward (+)

1607445 .. 1608852

Gene Structure 1408 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g01240.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGATAGCTCTCTTATTTTATTTTATTTTTTTAATAAAACAAGTCTCTTATTACAACAACCAATACTTGCGTCTTTTTTCCACGCAGCCCACAATCCACCATTTGATTCAACTGCGTTTCTCTGTGTCTCACTCTGAGTCTTCGATCTGTGCATTTCCTCTTGTCCAGTTCTCCGGCAGCCCACCGCAAAAAGCCATGGACGCTCTCCCGCAGGACACGCTCCACCAGATCTTCTCGAGCCTTCCCCTCCGTCAGATCATGATCTGCCGCTCACTCTCCAAGTTCTTCAACCAACTCCTCACCTCACCCTCCTTCCTCCACCTCATCTCCACCCAATCTCCGCCCCTCAACCTCCTCGCCCTCCGCCCTCCCCACCACCGGCACCTGTCCTCCCCATCCTGCCTCCACGTCTTCGACCCCGACCTCAACCAATGGCTCAGATTCCCCCTCGATTTCCTCCCCTTCCGCTCCCCACACCCCGTCGCCTCCTCACTCGGCCTCGTCTACCTCTGGGGCGACTCGCCCGACCCGCCCGAGTCAACCAAGTCACTCGTCGTCTGCAACCCTCTGACTCGCCAGTTCCGAGTCCTCCCTCAGCTCGGCTCAGCCTGGTCGCGACACGGCTCGGTCCTCGTTGACTCCCAAACCCGAGTCATGGTCCTCACTGAGCTCGCTGCACTCTACTTCTCCGGTTCGAACCAGTGGCTCAAGTTCTCATCGAACCTCCCTTCAAAACCCAGAAGCCCAATTTTGGTGTTCGACTCGGTTTACGCCCTGTGCGATGTGGGTTCGCCGTGGCGGTGTCAATGGAAGCTGTTCCATTGCACAATTTCGAAGCTTAAGACCTCGCAGACGTGGACTCGGCTCGAAAAGCACGAGTGGGGGGACGTCTTCGACATCCTGAAGCGACCCCGTTTGGTTCGGGGGAATGGAAACAAGGTGCTGATGGTTGGTGGGTTGAAGTCTTCTTTCTTGCTCAACGCTTCGTGTTCGACGATTTTGATTCTGCGGCTGGACTTGGATAGCTTGGAGTGGGACGAGGCGGGCCGAATGCCGGTGGAGATGTTCAGGTGCTTTCAAGAGTCGAGCAAGTTCAAGGTGTTTGGTGGCGGCGATAAGGTTTGTTTTTCGGCAAAGAGAATTGGGAGATTGGCTTTGTGGGACCGTTGTTCCGGGAAAGCCGAGTGGCGGTGGATTGATGGGGTTCCCGGAAGTGGCGATGGGCTTTCCCGCGGTTTCGTTTTTGAGGCCACGCTCACTGCATTTCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031146 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box Kelch_2

Protein Analysis

424

Amino Acids

47.99

Weight (kDa)

9.4

Isoelectric Point (pI)

52.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	68 - 107	8e-07	F-box domain
Beta-prop_KIB1-4	PF03478	147 - 351	5.2e-06	KIB1-4 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76920
fragaria_vesca	FvH4_2g14200 FvH4_2g14200 FvH4_3g14282
malus_domestica	MD05G1021300.v1.1 MD10G1022100.v1.1
prunus_persica	Prupe.8G025000_v2.0.a1 Prupe.8G025000_v2.0.a1
pyrus_communis	pycom05g01240 pycom10g01530
rosa_chinensis	RchiOBHm_Chr2g0119661 RchiOBHm_Chr6g0276341
rosa_laevigata	RLG00000004054 RLG00000013402 RLG00000034851
rosa_multiflora	Rmu_sc0000825.1_g000018
rosa_roxburghii	Rroxscaffold_7G00192360
rosa_rugosa	Rorug02G0346400 Rorug06G0100300.1 Rorug06G0193700 Rorug06G0302000
rosa_samantha	Rh4DG017200 Rh6AG211400 Rh6BG215800 Rh6CG218000 Rh6DG208100
rosa_wichuraiana	Rw6G018470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	891
AccB1I	GGYRCC	1 cut(s)	381
AccBSI	CCGCTC	2 cut(s)	270, 468
AccI	GTMKAC	1 cut(s)	504
AccII	CGCG	2 cut(s)	616, 1233
AcuI	CTGAAG	1 cut(s)	923
AcyI	GRCGYC	1 cut(s)	888
AfiI	CCNNNNNNNGG	7 cut(s)	378, 599, 655, 751, 1054, 1188, 1214
AflII	CTTAAG	1 cut(s)	839
AgsI	TTSAA	6 cut(s)	110, 289, 732, 961, 1079, 1096
AjiI	CACGTC	2 cut(s)	409, 855
AjnI	CCWGG	1 cut(s)	608
AluBI	AGCT	6 cut(s)	8, 598, 670, 814, 838, 1026
AluI	AGCT	6 cut(s)	8, 598, 670, 814, 838, 1026
Alw21I	GWGCWC	1 cut(s)	672
Alw26I	GTCTC	2 cut(s)	49, 132
Ama87I	CYCGRG	3 cut(s)	235, 533, 648
AoxI	GGCC	3 cut(s)	496, 1045, 1248
ApeKI	GCWGC	3 cut(s)	85, 177, 674
Asp700I	GAANNNNTTC	1 cut(s)	815
AspS9I	GGNCC	4 cut(s)	628, 658, 1045, 1162
AsuC2I	CCSGG	2 cut(s)	1174, 1209
AsuHPI	GGTGA	2 cut(s)	292, 297
AsuII	TTCGAA	2 cut(s)	695, 833
AvaI	CYCGRG	3 cut(s)	235, 533, 648
AvaII	GGWCC	3 cut(s)	628, 658, 1162
BanI	GGYRCC	1 cut(s)	381
BanII	GRGCYC	1 cut(s)	672
BauI	CACGAG	1 cut(s)	875
BbsI	GAAGAC	4 cut(s)	132, 403, 883, 957
Bbv12I	GWGCWC	1 cut(s)	672
BbvCI	CCTCAGC	1 cut(s)	594
BbvI	GCAGC	3 cut(s)	97, 189, 661
BccI	CCATC	4 cut(s)	403, 940, 1193, 1214
BceAI	ACGGC	2 cut(s)	637, 778
BciT130I	CCWGG	1 cut(s)	610
BcnI	CCSGG	2 cut(s)	1174, 1209
BcoDI	GTCTC	2 cut(s)	49, 132
BfrI	CTTAAG	1 cut(s)	839
BglII	AGATCT	1 cut(s)	228
BisI	GCNGC	6 cut(s)	86, 178, 268, 675, 1010, 1111
BlpI	GCTNAGC	1 cut(s)	604
BlsI	GCNGC	6 cut(s)	87, 179, 269, 676, 1011, 1112
Bme1390I	CCNGG	3 cut(s)	610, 1174, 1209
Bme18I	GGWCC	3 cut(s)	628, 658, 1162
BmeT110I	CYCGRG	3 cut(s)	235, 533, 648
BmgBI	CACGTC	2 cut(s)	409, 855
BmgT120I	GGNCC	4 cut(s)	628, 658, 1045, 1162
BmiI	GGNNCC	3 cut(s)	383, 1163, 1206
BmrFI	CCNGG	3 cut(s)	610, 1174, 1209
BpiI	GAAGAC	4 cut(s)	132, 403, 883, 957
Bpu10I	CCTNAGC	1 cut(s)	594
Bpu1102I	GCTNAGC	1 cut(s)	604
Bpu14I	TTCGAA	2 cut(s)	695, 833
BpuEI	CTTGAG	1 cut(s)	692
BpuMI	CCSGG	2 cut(s)	1174, 1209
BsaBI	GATNNNNATC	1 cut(s)	260
BsaHI	GRCGYC	1 cut(s)	888
BsaJI	CCNNGG	3 cut(s)	195, 794, 1231
BsaWI	WCCGGW	1 cut(s)	689
BsaXI	ACNNNNNCTCC	6 cut(s)	311, 322, 341, 352, 374, 404
Bsc4I	CCNNNNNNNGG	7 cut(s)	378, 599, 655, 751, 1054, 1188, 1214
Bse118I	RCCGGY	2 cut(s)	378, 1054
Bse1I	ACTGG	3 cut(s)	166, 577, 700
Bse3DI	GCAATG	1 cut(s)	820
Bse8I	GATNNNNATC	1 cut(s)	260
BseBI	CCWGG	1 cut(s)	610
BseDI	CCNNGG	3 cut(s)	195, 794, 1231
BseGI	GGATG	2 cut(s)	395, 897
BseJI	GATNNNNATC	1 cut(s)	260
BseLI	CCNNNNNNNGG	7 cut(s)	378, 599, 655, 751, 1054, 1188, 1214
BseMI	GCAATG	1 cut(s)	820
BseMII	CTCAG	5 cut(s)	126, 451, 608, 618, 657
BseNI	ACTGG	3 cut(s)	166, 577, 700
BseRI	GAGGAG	3 cut(s)	287, 344, 478
BseXI	GCAGC	3 cut(s)	97, 189, 661
BsgI	GTGCAG	1 cut(s)	660
Bsh1236I	CGCG	2 cut(s)	616, 1233
BshFI	GGCC	3 cut(s)	498, 1047, 1250
BshNI	GGYRCC	1 cut(s)	381
BsiHKAI	GWGCWC	1 cut(s)	672
BsiHKCI	CYCGRG	3 cut(s)	235, 533, 648
BsiSI	CCGG	6 cut(s)	174, 379, 690, 1055, 1173, 1209
BslFI	GGGAC	3 cut(s)	899, 1049, 1175
BslI	CCNNNNNNNGG	7 cut(s)	378, 599, 655, 751, 1054, 1188, 1214
BsmAI	GTCTC	2 cut(s)	49, 132
BsmFI	GGGAC	3 cut(s)	899, 1049, 1175
BsmI	GAATGC	1 cut(s)	1056
BsnI	GGCC	3 cut(s)	498, 1047, 1250
BsoBI	CYCGRG	3 cut(s)	235, 533, 648
Bsp119I	TTCGAA	2 cut(s)	695, 833
Bsp1286I	GDGCHC	1 cut(s)	672
Bsp143I	GATC	4 cut(s)	144, 228, 255, 261
Bsp1720I	GCTNAGC	1 cut(s)	604
Bsp19I	CCATGG	1 cut(s)	195
Bsp68I	TCGCGA	1 cut(s)	616
BspANI	GGCC	3 cut(s)	498, 1047, 1250
BspCNI	CTCAG	5 cut(s)	127, 450, 607, 617, 658
BspFNI	CGCG	2 cut(s)	616, 1233
BspHI	TCATGA	1 cut(s)	258
BspLI	GGNNCC	3 cut(s)	383, 1163, 1206
BspT104I	TTCGAA	2 cut(s)	695, 833
BspT107I	GGYRCC	1 cut(s)	381
BspTI	CTTAAG	1 cut(s)	839
BsrBI	CCGCTC	2 cut(s)	270, 468
BsrDI	GCAATG	1 cut(s)	820
BsrFI	RCCGGY	2 cut(s)	378, 1054
BsrI	ACTGG	3 cut(s)	166, 577, 700
BssAI	RCCGGY	2 cut(s)	378, 1054
BssECI	CCNNGG	3 cut(s)	195, 794, 1231
BssMI	GATC	4 cut(s)	144, 228, 255, 261
BssNI	GRCGYC	1 cut(s)	888
BssSI	CACGAG	1 cut(s)	875
BssT1I	CCWWGG	1 cut(s)	195
Bst2BI	CACGAG	1 cut(s)	875
Bst2UI	CCWGG	1 cut(s)	610
Bst4CI	ACNGT	1 cut(s)	1166
BstACI	GRCGYC	1 cut(s)	888
BstAFI	CTTAAG	1 cut(s)	839
BstBI	TTCGAA	2 cut(s)	695, 833
BstC8I	GCNNGC	2 cut(s)	672, 1045
BstDEI	CTNAG	5 cut(s)	135, 437, 594, 604, 666
BstDSI	CCRYGG	3 cut(s)	195, 794, 1231
BstF5I	GGATG	2 cut(s)	395, 897
BstFNI	CGCG	2 cut(s)	616, 1233
BstKTI	GATC	4 cut(s)	147, 231, 258, 264
BstMAI	GTCTC	2 cut(s)	49, 132
BstMBI	GATC	4 cut(s)	144, 228, 255, 261
BstMWI	GCNNNNNNNGC	4 cut(s)	200, 217, 529, 871
BstNI	CCWGG	1 cut(s)	610
BstSCI	CCNGG	3 cut(s)	608, 1172, 1207
BstUI	CGCG	2 cut(s)	616, 1233
BstV1I	GCAGC	3 cut(s)	97, 189, 661
BstV2I	GAAGAC	4 cut(s)	132, 403, 883, 957
BstX2I	RGATCY	1 cut(s)	228
BstYI	RGATCY	1 cut(s)	228
BsuRI	GGCC	3 cut(s)	498, 1047, 1250
BtgI	CCRYGG	3 cut(s)	195, 794, 1231
BtgZI	GCGATG	2 cut(s)	795, 1233
BtrI	CACGTC	2 cut(s)	409, 855
BtsCI	GGATG	2 cut(s)	395, 897
BtsI	GCAGTG	1 cut(s)	1257
BtsIMutI	CAGTG	3 cut(s)	663, 707, 1257
BtuMI	TCGCGA	1 cut(s)	616
Cac8I	GCNNGC	2 cut(s)	672, 1045
CciI	TCATGA	1 cut(s)	258
Cfr10I	RCCGGY	2 cut(s)	378, 1054
Cfr13I	GGNCC	4 cut(s)	628, 658, 1045, 1162
Cfr42I	CCGCGG	1 cut(s)	1234
CseI	GACGC	2 cut(s)	59, 209
CviAII	CATG	3 cut(s)	196, 259, 655
DdeI	CTNAG	5 cut(s)	135, 437, 594, 604, 666
DpnI	GATC	4 cut(s)	146, 230, 257, 263
DpnII	GATC	4 cut(s)	144, 228, 255, 261
EciI	GGCGGA	2 cut(s)	330, 353
Ecl136II	GAGCTC	1 cut(s)	670
Eco130I	CCWWGG	1 cut(s)	195
Eco24I	GRGCYC	1 cut(s)	672
Eco47I	GGWCC	3 cut(s)	628, 658, 1162
Eco53kI	GAGCTC	1 cut(s)	670
Eco57I	CTGAAG	1 cut(s)	923
Eco88I	CYCGRG	3 cut(s)	235, 533, 648
EcoICRI	GAGCTC	1 cut(s)	670
EcoRII	CCWGG	1 cut(s)	608
EcoT14I	CCWWGG	1 cut(s)	195
EcoT38I	GRGCYC	1 cut(s)	672
ErhI	CCWWGG	1 cut(s)	195
FaeI	CATG	3 cut(s)	199, 262, 658
FaiI	YATR	3 cut(s)	197, 260, 656
FaqI	GGGAC	3 cut(s)	899, 1049, 1175
FatI	CATG	3 cut(s)	195, 258, 654
FauI	CCCGC	4 cut(s)	216, 537, 1036, 1238
FblI	GTMKAC	1 cut(s)	504
Fnu4HI	GCNGC	6 cut(s)	86, 178, 268, 675, 1010, 1111
FokI	GGATG	2 cut(s)	382, 884
FriOI	GRGCYC	1 cut(s)	672
Fsp4HI	GCNGC	6 cut(s)	86, 178, 268, 675, 1010, 1111
GluI	GCNGC	6 cut(s)	86, 178, 268, 675, 1010, 1111
HaeIII	GGCC	3 cut(s)	498, 1047, 1250
HapII	CCGG	6 cut(s)	174, 379, 690, 1055, 1173, 1209
HgaI	GACGC	2 cut(s)	59, 209
Hin1I	GRCGYC	1 cut(s)	888
Hin1II	CATG	3 cut(s)	199, 262, 658
HincII	GTYRAC	2 cut(s)	540, 637
HindII	GTYRAC	2 cut(s)	540, 637
HindIII	AAGCTT	1 cut(s)	836
HpaII	CCGG	6 cut(s)	174, 379, 690, 1055, 1173, 1209
HphI	GGTGA	2 cut(s)	292, 297
Hpy166II	GTNNAC	5 cut(s)	505, 540, 637, 769, 858
Hpy188I	TCNGA	5 cut(s)	136, 255, 440, 570, 584
Hpy188III	TCNNGA	5 cut(s)	235, 259, 615, 901, 1079
Hpy8I	GTNNAC	5 cut(s)	505, 540, 637, 769, 858
Hpy99I	CGWCG	3 cut(s)	485, 557, 997
HpyAV	CCTTC	4 cut(s)	251, 322, 472, 738
HpyCH4III	ACNGT	1 cut(s)	1166
HpyCH4IV	ACGT	3 cut(s)	408, 854, 888
HpyCH4V	TGCA	5 cut(s)	152, 561, 677, 825, 1262
HpyF10VI	GCNNNNNNNGC	4 cut(s)	200, 217, 529, 871
HpyF3I	CTNAG	5 cut(s)	135, 437, 594, 604, 666
HpySE526I	ACGT	3 cut(s)	408, 854, 888
Hsp92I	GRCGYC	1 cut(s)	888
Hsp92II	CATG	3 cut(s)	199, 262, 658
KspI	CCGCGG	1 cut(s)	1234
Kzo9I	GATC	4 cut(s)	144, 228, 255, 261
LmnI	GCTCC	2 cut(s)	225, 473
Lsp1109I	GCAGC	3 cut(s)	97, 189, 661
MaeII	ACGT	3 cut(s)	408, 854, 888
MaeIII	GTNAC	1 cut(s)	546
MalI	GATC	4 cut(s)	146, 230, 257, 263
MbiI	CCGCTC	2 cut(s)	270, 468
MboI	GATC	4 cut(s)	144, 228, 255, 261
MboII	GAAGA	6 cut(s)	132, 223, 277, 403, 883, 957
MflI	RGATCY	1 cut(s)	228
MhlI	GDGCHC	1 cut(s)	672
MluCI	AATT	3 cut(s)	747, 828, 1140
MroXI	GAANNNNTTC	1 cut(s)	815
MseI	TTAA	2 cut(s)	32, 840
MslI	CAYNNNNRTG	1 cut(s)	1267
MspA1I	CMGCKG	1 cut(s)	1233
MspCI	CTTAAG	1 cut(s)	839
MspI	CCGG	6 cut(s)	174, 379, 690, 1055, 1173, 1209
MspR9I	CCNGG	3 cut(s)	610, 1174, 1209
Mva1269I	GAATGC	1 cut(s)	1056
MvaI	CCWGG	1 cut(s)	610
MvnI	CGCG	2 cut(s)	616, 1233
MwoI	GCNNNNNNNGC	4 cut(s)	200, 217, 529, 871
NciI	CCSGG	2 cut(s)	1174, 1209
NcoI	CCATGG	1 cut(s)	195
NdeII	GATC	4 cut(s)	144, 228, 255, 261
NlaIII	CATG	3 cut(s)	199, 262, 658
NlaIV	GGNNCC	3 cut(s)	383, 1163, 1206
NmeAIII	GCCGAG	4 cut(s)	474, 579, 841, 1207
NmuCI	GTSAC	1 cut(s)	546
NruI	TCGCGA	1 cut(s)	616
NspV	TTCGAA	2 cut(s)	695, 833
PaeR7I	CTCGAG	1 cut(s)	235
PagI	TCATGA	1 cut(s)	258
PcsI	WCGNNNNNNNCGW	4 cut(s)	580, 777, 873, 992
PctI	GAATGC	1 cut(s)	1056
PdmI	GAANNNNTTC	1 cut(s)	815
PfeI	GAWTC	3 cut(s)	106, 441, 1003
PfoI	TCCNGGA	2 cut(s)	1172, 1207
PkrI	GCNGC	6 cut(s)	87, 179, 269, 676, 1011, 1112
Psp124BI	GAGCTC	1 cut(s)	672
Psp6I	CCWGG	1 cut(s)	608
PspGI	CCWGG	1 cut(s)	608
PspN4I	GGNNCC	3 cut(s)	383, 1163, 1206
PspPI	GGNCC	4 cut(s)	628, 658, 1045, 1162
PsuI	RGATCY	1 cut(s)	228
RruI	TCGCGA	1 cut(s)	616
RseI	CAYNNNNRTG	1 cut(s)	1267
SacI	GAGCTC	1 cut(s)	672
SacII	CCGCGG	1 cut(s)	1234
SaqAI	TTAA	2 cut(s)	32, 840
SatI	GCNGC	6 cut(s)	86, 178, 268, 675, 1010, 1111
Sau3AI	GATC	4 cut(s)	144, 228, 255, 261
Sau96I	GGNCC	4 cut(s)	628, 658, 1045, 1162
ScrFI	CCNGG	3 cut(s)	610, 1174, 1209
SduI	GDGCHC	1 cut(s)	672
Sfr274I	CTCGAG	1 cut(s)	235
Sfr303I	CCGCGG	1 cut(s)	1234
SfuI	TTCGAA	2 cut(s)	695, 833
SgrBI	CCGCGG	1 cut(s)	1234
SinI	GGWCC	3 cut(s)	628, 658, 1162
SlaI	CTCGAG	1 cut(s)	235
SmiMI	CAYNNNNRTG	1 cut(s)	1267
SmlI	CTYRAG	3 cut(s)	235, 707, 839
SmoI	CTYRAG	3 cut(s)	235, 707, 839
Sse9I	AATT	3 cut(s)	747, 828, 1140
SstI	GAGCTC	1 cut(s)	672
StyD4I	CCNGG	3 cut(s)	608, 1172, 1207
StyI	CCWWGG	1 cut(s)	195
TaaI	ACNGT	1 cut(s)	1166
TaiI	ACGT	3 cut(s)	411, 857, 891
TaqII	GACCGA	1 cut(s)	616
TasI	AATT	3 cut(s)	747, 828, 1140
TauI	GCSGC	3 cut(s)	270, 1012, 1113
TfiI	GAWTC	3 cut(s)	106, 441, 1003
Tru1I	TTAA	2 cut(s)	32, 840
Tru9I	TTAA	2 cut(s)	32, 840
TscAI	CASTG	3 cut(s)	670, 707, 1264
TseFI	GTSAC	1 cut(s)	546
TseI	GCWGC	3 cut(s)	85, 177, 674
Tsp45I	GTSAC	1 cut(s)	546
TspGWI	ACGGA	2 cut(s)	239, 1257
TspRI	CASTG	3 cut(s)	670, 707, 1264
Vha464I	CTTAAG	1 cut(s)	839
VpaK11BI	GGWCC	3 cut(s)	628, 658, 1162
XhoI	CTCGAG	1 cut(s)	235
XmiI	GTMKAC	1 cut(s)	504
XmnI	GAANNNNTTC	1 cut(s)	815
ZraI	GACGTC	1 cut(s)	889

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.