Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003856

Physical Location & Seq

Genomic Coordinates Forward (+)

7273929 .. 7277419

Gene Structure 3491 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1053800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2022 bp

ATGTGGGATTCTGAGACTGAGTTGGTTGGAGGAAGAGATTATGGGAATGGAGTTCTGAGTTCAACCAAGCAGAGTGTTAAGACTGATGGCTTTGAGCTCAAAGGCAACTCTTGGTATGTTGCAACTGATATCCCAAGTGACCTGCTGGTTCAAGTTGGGGATGTCAATTTTCACTTGCACAAGTATCCCCTGCTTTCTAGGAGCGGAAAGATCAACAGAGTTATGTATGAATTACGCGACCCGGACTTGAGTAAGATGGTTTTGGATGATGTTCCTGGTGGACCGGAGGCCTTCGAGCTAGCTGCAAAATTCTGCTATGGAATTGCAGTTGACCTAACAGCAGCAAATATTTCGGGCCTGAGATGTGCTGCAGAGTACCTTGAAATGACAGAGGACTTGGAAGAAGGCAACCTTATTTTCAAAACCGAGGCATTTCTTAGCTATGTGGTTTTATCCTCGTGGAGAGACTCCATTGTTGTGTTGAAGAGCTGTGAGAAGCTGTCACCGTGGGCAGAAAACCTTCAAATTGTCCGAAGATGCAGCGAGTCTATTGCCTGGAAAGCTTGTGCTAATCCGAAAGGGATAAGATGGGCATACACCGGAAAGCCAACAAAAGTTTCTAGCCCAAATTGGGGTGACATGAAGGACTCAAGTCCAAGTAGAAGCCAGCAGGTCCCTCCCGATTGGTGGTTTGAAGATGTTTCAATTCTTAGGATTGATCACTTTGTCAGGGTTATCACCGCAATTAAGGTAAAGGGGATGAGATTTGAACTGATTGGAGCTTCAATTATGCACTACGCATCTAAGTGGCTTCCAGGTTTGATCAACGAAGGAACGGGTGCTGCAGATGAAGGAAGCAACAGCAGCAATAGTACAAGTAATAGTGGCAGTAGTTGGAAAGGTGGACTTCATATGATTGTGGCAGGAAATAAAAATGAGCCTGCAAATGTTCAGGCCAAAAATCAACGGATGATCATTGAGAGCCTTATCAGCATAATTCCACCACAGAAGGATAGTGTCTCGTGCAGCTTCCTGCTTCGGCTTTTGAGAATGGCAAACATGTTGAAAGTAGCACCTGCTTTGGTAACTGAGTTGGAAAAACGGGTGGGAATGCAGTTCGAACAGGCTACATTGGCGGATCTTCTTATTCCTTCGTACAATAAATGTGAAACTATGTACGACGTGGATCTTGTTCAAAGGCTTCTGGAGCACTTTCTGATTCAGGAACAGACAGAAATTTCGAGTCCAAGGCCACAATCTTTTCCTGGGAAGCACTATGATGACCTTCAAAGGGGTACCGGCCCAAATGCTAAGATGAGGGTAGCTAGGCTTGTCGATAGTTATCTTACAGAGGTGTCCAGAGATAGAAGCCTCTCCCTAACAAAGTTTCAGGTACTGGCAGAAGCTTTGCCCGAATCTGCAAGGATCTGCGATGATGGACTTTATAGAGCAATCGATTCCTATCTTAAGGCCCATCCAACACTTTCTGAGCACGAAAGGAAGAGGCTTTGCCGGGTGATGGATTGCCAAAAGCTATCAATTGATGCCTGCATGCATGCTGCCCAAAATGAAAGGCTCCCACTTAGAGTGGTAGTGCAAGTCCTTTTCTCTGAACAAGTTAAAATAAGCAATGCATTAGCCACAAGCTCCCTCAAAGAAGCTGGAGACTCTCAGTTTCAGCCAATGGTTTCCAATCGAAAAACACTACTGGAAGGGACCCCGCAGTCATTCCAAGAAGGATGGGCTGCCGCTAAAAAAGACATTAACACGGTGAAATTCGAACTGGAGAGTGTGAAAGCCAAGTACGTAGAGCTCCAGCATGACATGGAGAATTTGCAGAGACAGTTTGATAAGATGTCAAAGCACAAACAGACATCAGCATGGAGCAGTGGGTGGAAGAAACTAAGCAAACTCACAAAGAACACAAATCTAGAGAACCAGAACATCGGGCTGGAGCACACAACGGCCGCAGATCAGCTGAGTAGAAAGACACCTAGGAGGTGGAGAAATTCCATCTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0009987 GO:0016020 GO:0016567 GO:0019538 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0070647 GO:0071704 GO:0071944 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB NPH3

Protein Analysis

674

Amino Acids

75.57

Weight (kDa)

8.13

Isoelectric Point (pI)

47.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPH3	PF03000	228 - 517	2e-99	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003856)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64330 AT5G64330 AT5G64330
fragaria_vesca	FvH4_5g25720 FvH4_5g25720 FvH4_5g25720
malus_domestica	MD05G1047100.v1.1 MD10G1053800.v1.1
prunus_persica	Prupe.8G067500_v2.0.a1 Prupe.8G067500_v2.0.a1
pyrus_communis	pycom10g04060
rosa_chinensis	RchiOBHm_Chr6g0271301 RchiOBHm_Chr7g0216061
rosa_laevigata	RLG00000002597 RLG00000013726
rosa_multiflora	Rmu_sc0002356.1_g000004 Rmu_sc0004866.1_g000011
rosa_roxburghii	Rroxscaffold_3G00243680 Rroxscaffold_7G00197120
rosa_rugosa	Rorug06G0059500 Rorug06G0059600 Rorug06G0059700 Rorug07G0158400 Rorug07G0158500 Rorug07G0158600
rosa_samantha	Rh6AG179400 Rh6BG182500 Rh6CG179400 Rh6DG171100 Rh7AG295600 Rh7CG316000
rosa_wichuraiana	Rw6G015410 Rw6G015430 Rw7G025140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1084
AasI	GACNNNNNNGTC	1 cut(s)	1723
Acc36I	ACCTGC	3 cut(s)	150, 661, 1084
Acc65I	GGTACC	1 cut(s)	1295
AccB1I	GGYRCC	1 cut(s)	1295
AccBSI	CCGCTC	1 cut(s)	204
AccII	CGCG	1 cut(s)	237
AciI	CCGC	6 cut(s)	204, 741, 1136, 1721, 1749, 1968
AclWI	GGATC	3 cut(s)	1146, 1194, 1433
AcoI	YGGCCR	1 cut(s)	1965
AcsI	RAATTY	5 cut(s)	308, 1236, 1775, 1831, 2008
AfaI	GTAC	7 cut(s)	377, 874, 1157, 1178, 1297, 1395, 1805
AfiI	CCNNNNNNNGG	5 cut(s)	631, 632, 687, 1291, 1519
AflII	CTTAAG	1 cut(s)	1466
AflIII	ACRYGT	1 cut(s)	1059
AjiI	CACGTC	1 cut(s)	1183
AjnI	CCWGG	4 cut(s)	274, 554, 814, 1264
AjuI	GAANNNNNNNTTGG	2 cut(s)	601, 633
Alw21I	GWGCWC	5 cut(s)	99, 1212, 1494, 1815, 1959
Alw26I	GTCTC	5 cut(s)	8, 459, 1024, 1659, 1834
AlwI	GGATC	3 cut(s)	1146, 1194, 1433
AlwNI	CAGNNNCTG	1 cut(s)	1396
AoxI	GGCC	7 cut(s)	288, 355, 954, 1250, 1300, 1470, 1965
ApeKI	GCWGC	9 cut(s)	302, 341, 368, 540, 842, 864, 1026, 1559, 1745
ApoI	RAATTY	5 cut(s)	308, 1236, 1775, 1831, 2008
ArsI	GACNNNNNNTTYG	2 cut(s)	1222, 1254
Asp700I	GAANNNNTTC	1 cut(s)	519
Asp718I	GGTACC	1 cut(s)	1295
AspA2I	CCTAGG	1 cut(s)	1994
AspS9I	GGNCC	6 cut(s)	281, 355, 673, 1301, 1471, 1716
AsuC2I	CCSGG	2 cut(s)	242, 1514
AsuHPI	GGTGA	5 cut(s)	495, 647, 730, 1528, 1783
AsuII	TTCGAA	2 cut(s)	1119, 1779
AsuNHI	GCTAGC	1 cut(s)	298
AvaII	GGWCC	3 cut(s)	281, 673, 1716
AvrII	CCTAGG	1 cut(s)	1994
BanI	GGYRCC	1 cut(s)	1295
BanII	GRGCYC	2 cut(s)	99, 1815
BauI	CACGAG	2 cut(s)	457, 1021
Bbv12I	GWGCWC	5 cut(s)	99, 1212, 1494, 1815, 1959
BbvI	GCAGC	9 cut(s)	289, 353, 355, 552, 829, 876, 1038, 1546, 1732
BccI	CCATC	8 cut(s)	80, 250, 582, 1430, 1482, 1513, 1734, 2021
BceAI	ACGGC	1 cut(s)	1980
BcgI	CGANNNNNNTGC	6 cut(s)	284, 318, 333, 367, 533, 567
BciT130I	CCWGG	4 cut(s)	276, 556, 816, 1266
BciVI	GTATCC	1 cut(s)	195
BclI	TGATCA	3 cut(s)	718, 822, 972
BcnI	CCSGG	2 cut(s)	242, 1514
BcoDI	GTCTC	5 cut(s)	8, 459, 1024, 1659, 1834
BfaI	CTAG	6 cut(s)	198, 299, 621, 1326, 1931, 1995
BfmI	CTRYAG	2 cut(s)	369, 843
BfrI	CTTAAG	1 cut(s)	1466
BfuAI	ACCTGC	3 cut(s)	150, 661, 1084
BfuI	GTATCC	1 cut(s)	195
BlnI	CCTAGG	1 cut(s)	1994
Bme1390I	CCNGG	6 cut(s)	242, 276, 556, 816, 1266, 1514
Bme18I	GGWCC	3 cut(s)	281, 673, 1716
BmgBI	CACGTC	1 cut(s)	1183
BmgT120I	GGNCC	6 cut(s)	281, 355, 673, 1301, 1471, 1716
BmiI	GGNNCC	5 cut(s)	675, 1297, 1577, 1717, 1718
BmrFI	CCNGG	6 cut(s)	242, 276, 556, 816, 1266, 1514
BmsI	GCATC	3 cut(s)	527, 809, 1534
BmtI	GCTAGC	1 cut(s)	302
BoxI	GACNNNNGTC	1 cut(s)	651
BpmI	CTGGAG	5 cut(s)	1226, 1683, 1799, 1805, 1973
Bpu14I	TTCGAA	2 cut(s)	1119, 1779
BpuEI	CTTGAG	2 cut(s)	268, 634
BpuMI	CCSGG	2 cut(s)	242, 1514
Bsa29I	ATCGAT	1 cut(s)	1455
BsaAI	YACGTR	1 cut(s)	1807
BsaBI	GATNNNNATC	2 cut(s)	1341, 1461
BsaJI	CCNNGG	5 cut(s)	426, 506, 1247, 1265, 1994
BsaWI	WCCGGW	2 cut(s)	283, 599
Bsc4I	CCNNNNNNNGG	5 cut(s)	631, 632, 687, 1291, 1519
Bse118I	RCCGGY	1 cut(s)	1298
Bse1I	ACTGG	3 cut(s)	1401, 1713, 1788
Bse3DI	GCAATG	1 cut(s)	1636
Bse8I	GATNNNNATC	2 cut(s)	1341, 1461
BseBI	CCWGG	4 cut(s)	276, 556, 816, 1266
BseCI	ATCGAT	1 cut(s)	1455
BseDI	CCNNGG	5 cut(s)	426, 506, 1247, 1265, 1994
BseGI	GGATG	6 cut(s)	166, 271, 765, 975, 1474, 1745
BseJI	GATNNNNATC	2 cut(s)	1341, 1461
BseLI	CCNNNNNNNGG	5 cut(s)	631, 632, 687, 1291, 1519
BseMI	GCAATG	1 cut(s)	1636
BseMII	CTCAG	7 cut(s)	9, 47, 350, 1080, 1479, 1685, 1970
BseNI	ACTGG	3 cut(s)	1401, 1713, 1788
BseX3I	CGGCCG	1 cut(s)	1965
BseXI	GCAGC	9 cut(s)	289, 353, 355, 552, 829, 876, 1038, 1546, 1732
BsgI	GTGCAG	1 cut(s)	1045
Bsh1236I	CGCG	1 cut(s)	237
Bsh1285I	CGRYCG	1 cut(s)	1968
BshFI	GGCC	7 cut(s)	290, 357, 956, 1252, 1302, 1472, 1967
BshNI	GGYRCC	1 cut(s)	1295
BshVI	ATCGAT	1 cut(s)	1455
BsiEI	CGRYCG	1 cut(s)	1968
BsiHKAI	GWGCWC	5 cut(s)	99, 1212, 1494, 1815, 1959
BsiSI	CCGG	5 cut(s)	242, 284, 600, 1299, 1513
BslFI	GGGAC	2 cut(s)	659, 1729
BslI	CCNNNNNNNGG	5 cut(s)	631, 632, 687, 1291, 1519
BsmAI	GTCTC	5 cut(s)	8, 459, 1024, 1659, 1834
BsmFI	GGGAC	2 cut(s)	659, 1729
BsmI	GAATGC	1 cut(s)	1116
BsnI	GGCC	7 cut(s)	290, 357, 956, 1252, 1302, 1472, 1967
Bsp119I	TTCGAA	2 cut(s)	1119, 1779
Bsp1286I	GDGCHC	5 cut(s)	99, 1212, 1494, 1815, 1959
Bsp143I	GATC	8 cut(s)	210, 718, 822, 972, 1138, 1186, 1425, 1972
BspACI	CCGC	6 cut(s)	204, 741, 1136, 1721, 1749, 1968
BspANI	GGCC	7 cut(s)	290, 357, 956, 1252, 1302, 1472, 1967
BspCNI	CTCAG	8 cut(s)	4, 10, 48, 351, 1081, 1480, 1684, 1971
BspDI	ATCGAT	1 cut(s)	1455
BspFNI	CGCG	1 cut(s)	237
BspLI	GGNNCC	5 cut(s)	675, 1297, 1577, 1717, 1718
BspMAI	CTGCAG	2 cut(s)	373, 847
BspMI	ACCTGC	3 cut(s)	150, 661, 1084
BspOI	GCTAGC	1 cut(s)	302
BspPI	GGATC	3 cut(s)	1146, 1194, 1433
BspQI	GCTCTTC	1 cut(s)	479
BspT104I	TTCGAA	2 cut(s)	1119, 1779
BspT107I	GGYRCC	1 cut(s)	1295
BspTI	CTTAAG	1 cut(s)	1466
BsrBI	CCGCTC	1 cut(s)	204
BsrDI	GCAATG	1 cut(s)	1636
BsrFI	RCCGGY	1 cut(s)	1298
BsrI	ACTGG	3 cut(s)	1401, 1713, 1788
BssAI	RCCGGY	1 cut(s)	1298
BssECI	CCNNGG	5 cut(s)	426, 506, 1247, 1265, 1994
BssMI	GATC	8 cut(s)	210, 718, 822, 972, 1138, 1186, 1425, 1972
BssSI	CACGAG	2 cut(s)	457, 1021
BssT1I	CCWWGG	2 cut(s)	1247, 1994
Bst2BI	CACGAG	2 cut(s)	457, 1021
Bst2UI	CCWGG	4 cut(s)	276, 556, 816, 1266
Bst4CI	ACNGT	3 cut(s)	507, 1771, 1845
Bst6I	CTCTTC	3 cut(s)	28, 479, 1496
BstAFI	CTTAAG	1 cut(s)	1466
BstBAI	YACGTR	1 cut(s)	1807
BstBI	TTCGAA	2 cut(s)	1119, 1779
BstC8I	GCNNGC	6 cut(s)	300, 668, 942, 1549, 1553, 1557
BstDSI	CCRYGG	1 cut(s)	506
BstF5I	GGATG	6 cut(s)	166, 271, 765, 975, 1474, 1745
BstFNI	CGCG	1 cut(s)	237
BstKTI	GATC	8 cut(s)	213, 721, 825, 975, 1141, 1189, 1428, 1975
BstMAI	GTCTC	5 cut(s)	8, 459, 1024, 1659, 1834
BstMBI	GATC	8 cut(s)	210, 718, 822, 972, 1138, 1186, 1425, 1972
BstMCI	CGRYCG	1 cut(s)	1968
BstMWI	GCNNNNNNNGC	5 cut(s)	560, 864, 990, 1133, 1207
BstNI	CCWGG	4 cut(s)	276, 556, 816, 1266
BstNSI	RCATGY	3 cut(s)	1063, 1555, 1559
BstPAI	GACNNNNGTC	1 cut(s)	651
BstSCI	CCNGG	6 cut(s)	240, 274, 554, 814, 1264, 1512
BstSFI	CTRYAG	2 cut(s)	369, 843
BstSNI	TACGTA	1 cut(s)	1807
BstUI	CGCG	1 cut(s)	237
BstV1I	GCAGC	9 cut(s)	289, 353, 355, 552, 829, 876, 1038, 1546, 1732
BstX2I	RGATCY	3 cut(s)	1138, 1186, 1425
BstYI	RGATCY	3 cut(s)	1138, 1186, 1425
BstZI	CGGCCG	1 cut(s)	1965
Bsu15I	ATCGAT	1 cut(s)	1455
BsuI	GTATCC	1 cut(s)	195
BsuRI	GGCC	7 cut(s)	290, 357, 956, 1252, 1302, 1472, 1967
BsuTUI	ATCGAT	1 cut(s)	1455
BtgI	CCRYGG	1 cut(s)	506
BtgZI	GCGATG	1 cut(s)	1446
BtrI	CACGTC	1 cut(s)	1183
BtsCI	GGATG	6 cut(s)	166, 271, 765, 975, 1474, 1745
BtsI	GCAGTG	1 cut(s)	1894
BtsIMutI	CAGTG	1 cut(s)	1894
BveI	ACCTGC	3 cut(s)	150, 661, 1084
Cac8I	GCNNGC	6 cut(s)	300, 668, 942, 1549, 1553, 1557
CaiI	CAGNNNCTG	1 cut(s)	1396
Cfr10I	RCCGGY	1 cut(s)	1298
Cfr13I	GGNCC	6 cut(s)	281, 355, 673, 1301, 1471, 1716
ClaI	ATCGAT	1 cut(s)	1455
Csp6I	GTAC	7 cut(s)	376, 873, 1156, 1177, 1296, 1394, 1804
CviAII	CATG	7 cut(s)	640, 1060, 1552, 1556, 1820, 1825, 1881
CviQI	GTAC	7 cut(s)	376, 873, 1156, 1177, 1296, 1394, 1804
DpnI	GATC	8 cut(s)	212, 720, 824, 974, 1140, 1188, 1427, 1974
DpnII	GATC	8 cut(s)	210, 718, 822, 972, 1138, 1186, 1425, 1972
DrdI	GACNNNNNNGTC	1 cut(s)	1723
DseDI	GACNNNNNNGTC	1 cut(s)	1723
EaeI	YGGCCR	1 cut(s)	1965
EagI	CGGCCG	1 cut(s)	1965
Eam1104I	CTCTTC	3 cut(s)	28, 479, 1496
EarI	CTCTTC	3 cut(s)	28, 479, 1496
EciI	GGCGGA	1 cut(s)	1151
Ecl136II	GAGCTC	2 cut(s)	97, 1813
EclXI	CGGCCG	1 cut(s)	1965
Eco105I	TACGTA	1 cut(s)	1807
Eco130I	CCWWGG	2 cut(s)	1247, 1994
Eco147I	AGGCCT	1 cut(s)	290
Eco24I	GRGCYC	2 cut(s)	99, 1815
Eco32I	GATATC	1 cut(s)	130
Eco47I	GGWCC	3 cut(s)	281, 673, 1716
Eco52I	CGGCCG	1 cut(s)	1965
Eco53kI	GAGCTC	2 cut(s)	97, 1813
EcoICRI	GAGCTC	2 cut(s)	97, 1813
EcoO109I	RGGNCCY	2 cut(s)	673, 1716
EcoRII	CCWGG	4 cut(s)	274, 554, 814, 1264
EcoRV	GATATC	1 cut(s)	130
EcoT14I	CCWWGG	2 cut(s)	1247, 1994
EcoT22I	ATGCAT	2 cut(s)	1557, 1636
EcoT38I	GRGCYC	2 cut(s)	99, 1815
ErhI	CCWWGG	2 cut(s)	1247, 1994
FaeI	CATG	7 cut(s)	643, 1063, 1555, 1559, 1823, 1828, 1884
FalI	AAGNNNNNCTT	4 cut(s)	396, 428, 891, 923
FaqI	GGGAC	2 cut(s)	659, 1729
FatI	CATG	7 cut(s)	639, 1059, 1551, 1555, 1819, 1824, 1880
FauI	CCCGC	1 cut(s)	1728
FauNDI	CATATG	1 cut(s)	912
FbaI	TGATCA	3 cut(s)	718, 822, 972
FokI	GGATG	6 cut(s)	173, 278, 772, 982, 1461, 1752
FriOI	GRGCYC	2 cut(s)	99, 1815
FspBI	CTAG	6 cut(s)	198, 299, 621, 1326, 1931, 1995
GsuI	CTGGAG	5 cut(s)	1226, 1683, 1799, 1805, 1973
HaeIII	GGCC	7 cut(s)	290, 357, 956, 1252, 1302, 1472, 1967
HapII	CCGG	5 cut(s)	242, 284, 600, 1299, 1513
Hin1II	CATG	7 cut(s)	643, 1063, 1555, 1559, 1823, 1828, 1884
HincII	GTYRAC	1 cut(s)	331
HindII	GTYRAC	1 cut(s)	331
HindIII	AAGCTT	2 cut(s)	561, 1404
HinfI	GANTC	9 cut(s)	8, 467, 545, 647, 1219, 1243, 1415, 1457, 1667
HpaII	CCGG	5 cut(s)	242, 284, 600, 1299, 1513
HphI	GGTGA	5 cut(s)	495, 647, 730, 1528, 1783
Hpy166II	GTNNAC	3 cut(s)	281, 331, 905
Hpy188I	TCNGA	7 cut(s)	13, 57, 533, 576, 1218, 1489, 1612
Hpy188III	TCNNGA	6 cut(s)	680, 1205, 1223, 1359, 1931, 2019
Hpy8I	GTNNAC	3 cut(s)	281, 331, 905
Hpy99I	CGWCG	1 cut(s)	1184
HpyCH4III	ACNGT	3 cut(s)	507, 1771, 1845
HpyCH4IV	ACGT	2 cut(s)	1182, 1806
HpyF10VI	GCNNNNNNNGC	5 cut(s)	560, 864, 990, 1133, 1207
HpySE526I	ACGT	2 cut(s)	1182, 1806
Hsp92II	CATG	7 cut(s)	643, 1063, 1555, 1559, 1823, 1828, 1884
KflI	GGGWCCC	1 cut(s)	1716
KpnI	GGTACC	1 cut(s)	1299
Ksp22I	TGATCA	3 cut(s)	718, 822, 972
Kzo9I	GATC	8 cut(s)	210, 718, 822, 972, 1138, 1186, 1425, 1972
LguI	GCTCTTC	1 cut(s)	479
LmnI	GCTCC	8 cut(s)	201, 779, 1207, 1581, 1652, 1818, 1884, 1954
Lsp1109I	GCAGC	9 cut(s)	289, 353, 355, 552, 829, 876, 1038, 1546, 1732
LweI	GCATC	3 cut(s)	527, 809, 1534
MaeI	CTAG	6 cut(s)	198, 299, 621, 1326, 1931, 1995
MaeII	ACGT	2 cut(s)	1182, 1806
MaeIII	GTNAC	4 cut(s)	137, 501, 635, 1084
MalI	GATC	8 cut(s)	212, 720, 824, 974, 1140, 1188, 1427, 1974
MbiI	CCGCTC	1 cut(s)	204
MboI	GATC	8 cut(s)	210, 718, 822, 972, 1138, 1186, 1425, 1972
MboII	GAAGA	8 cut(s)	45, 413, 496, 546, 707, 1133, 1513, 1909
MfeI	CAATTG	1 cut(s)	1539
MflI	RGATCY	3 cut(s)	1138, 1186, 1425
MhlI	GDGCHC	5 cut(s)	99, 1212, 1494, 1815, 1959
MlyI	GAGTC	5 cut(s)	461, 554, 641, 1252, 1661
MmeI	TCCRAC	4 cut(s)	7, 875, 1074, 1502
Mph1103I	ATGCAT	2 cut(s)	1557, 1636
MroXI	GAANNNNTTC	1 cut(s)	519
MseI	TTAA	5 cut(s)	78, 747, 1467, 1620, 1764
MslI	CAYNNNNRTG	4 cut(s)	476, 805, 1278, 1879
MspA1I	CMGCKG	1 cut(s)	1978
MspCI	CTTAAG	1 cut(s)	1466
MspI	CCGG	5 cut(s)	242, 284, 600, 1299, 1513
MspR9I	CCNGG	6 cut(s)	242, 276, 556, 816, 1266, 1514
MunI	CAATTG	1 cut(s)	1539
Mva1269I	GAATGC	1 cut(s)	1116
MvaI	CCWGG	4 cut(s)	276, 556, 816, 1266
MvnI	CGCG	1 cut(s)	237
MwoI	GCNNNNNNNGC	5 cut(s)	560, 864, 990, 1133, 1207
NciI	CCSGG	2 cut(s)	242, 1514
NdeI	CATATG	1 cut(s)	912
NdeII	GATC	8 cut(s)	210, 718, 822, 972, 1138, 1186, 1425, 1972
NheI	GCTAGC	1 cut(s)	298
NlaIII	CATG	7 cut(s)	643, 1063, 1555, 1559, 1823, 1828, 1884
NlaIV	GGNNCC	5 cut(s)	675, 1297, 1577, 1717, 1718
NmuCI	GTSAC	3 cut(s)	137, 501, 635
NsiI	ATGCAT	2 cut(s)	1557, 1636
NspI	RCATGY	3 cut(s)	1063, 1555, 1559
NspV	TTCGAA	2 cut(s)	1119, 1779
PaeI	GCATGC	2 cut(s)	1555, 1559
PaqCI	CACCTGC	1 cut(s)	1084
PceI	AGGCCT	1 cut(s)	290
PciI	ACATGT	1 cut(s)	1059
PciSI	GCTCTTC	1 cut(s)	479
PctI	GAATGC	1 cut(s)	1116
PdmI	GAANNNNTTC	1 cut(s)	519
PfeI	GAWTC	4 cut(s)	8, 1219, 1415, 1457
PleI	GAGTC	5 cut(s)	461, 553, 641, 1251, 1661
PpsI	GAGTC	5 cut(s)	461, 553, 641, 1251, 1661
Ppu21I	YACGTR	1 cut(s)	1807
PpuMI	RGGWCCY	2 cut(s)	673, 1716
PscI	ACATGT	1 cut(s)	1059
PshAI	GACNNNNGTC	1 cut(s)	651
Psp124BI	GAGCTC	2 cut(s)	99, 1815
Psp5II	RGGWCCY	2 cut(s)	673, 1716
Psp6I	CCWGG	4 cut(s)	274, 554, 814, 1264
PspGI	CCWGG	4 cut(s)	274, 554, 814, 1264
PspN4I	GGNNCC	5 cut(s)	675, 1297, 1577, 1717, 1718
PspPI	GGNCC	6 cut(s)	281, 355, 673, 1301, 1471, 1716
PspPPI	RGGWCCY	2 cut(s)	673, 1716
PstI	CTGCAG	2 cut(s)	373, 847
PstNI	CAGNNNCTG	1 cut(s)	1396
PsuI	RGATCY	3 cut(s)	1138, 1186, 1425
PvuII	CAGCTG	1 cut(s)	1978
RsaI	GTAC	7 cut(s)	377, 874, 1157, 1178, 1297, 1395, 1805
RsaNI	GTAC	7 cut(s)	376, 873, 1156, 1177, 1296, 1394, 1804
RseI	CAYNNNNRTG	4 cut(s)	476, 805, 1278, 1879
SacI	GAGCTC	2 cut(s)	99, 1815
SapI	GCTCTTC	1 cut(s)	479
SaqAI	TTAA	5 cut(s)	78, 747, 1467, 1620, 1764
Sau3AI	GATC	8 cut(s)	210, 718, 822, 972, 1138, 1186, 1425, 1972
Sau96I	GGNCC	6 cut(s)	281, 355, 673, 1301, 1471, 1716
SchI	GAGTC	5 cut(s)	461, 554, 641, 1252, 1661
ScrFI	CCNGG	6 cut(s)	242, 276, 556, 816, 1266, 1514
SduI	GDGCHC	5 cut(s)	99, 1212, 1494, 1815, 1959
SfaNI	GCATC	3 cut(s)	527, 809, 1534
SfcI	CTRYAG	2 cut(s)	369, 843
SfuI	TTCGAA	2 cut(s)	1119, 1779
SinI	GGWCC	3 cut(s)	281, 673, 1716
SmiMI	CAYNNNNRTG	4 cut(s)	476, 805, 1278, 1879
SmlI	CTYRAG	3 cut(s)	247, 649, 1466
SmoI	CTYRAG	3 cut(s)	247, 649, 1466
SnaBI	TACGTA	1 cut(s)	1807
SphI	GCATGC	2 cut(s)	1555, 1559
SseBI	AGGCCT	1 cut(s)	290
SsiI	CCGC	6 cut(s)	204, 741, 1136, 1721, 1749, 1968
SspI	AATATT	1 cut(s)	349
SspMI	CTAG	6 cut(s)	198, 299, 621, 1326, 1931, 1995
SstI	GAGCTC	2 cut(s)	99, 1815
StuI	AGGCCT	1 cut(s)	290
StyD4I	CCNGG	6 cut(s)	240, 274, 554, 814, 1264, 1512
StyI	CCWWGG	2 cut(s)	1247, 1994
TaaI	ACNGT	3 cut(s)	507, 1771, 1845
TaiI	ACGT	2 cut(s)	1185, 1809
TaqI	TCGA	7 cut(s)	294, 1119, 1241, 1335, 1455, 1696, 1779
TatI	WGTACW	1 cut(s)	872
TauI	GCSGC	2 cut(s)	1751, 1970
TfiI	GAWTC	4 cut(s)	8, 1219, 1415, 1457
Tru1I	TTAA	5 cut(s)	78, 747, 1467, 1620, 1764
Tru9I	TTAA	5 cut(s)	78, 747, 1467, 1620, 1764
TscAI	CASTG	1 cut(s)	1894
TseFI	GTSAC	3 cut(s)	137, 501, 635
TseI	GCWGC	9 cut(s)	302, 341, 368, 540, 842, 864, 1026, 1559, 1745
Tsp45I	GTSAC	3 cut(s)	137, 501, 635
TspDTI	ATGAA	5 cut(s)	243, 656, 864, 899, 1584
TspGWI	ACGGA	1 cut(s)	982
TspRI	CASTG	1 cut(s)	1894
Vha464I	CTTAAG	1 cut(s)	1466
VpaK11BI	GGWCC	3 cut(s)	281, 673, 1716
XapI	RAATTY	5 cut(s)	308, 1236, 1775, 1831, 2008
XbaI	TCTAGA	1 cut(s)	1930
XceI	RCATGY	3 cut(s)	1063, 1555, 1559
XmaJI	CCTAGG	1 cut(s)	1994
XmnI	GAANNNNTTC	1 cut(s)	519
XspI	CTAG	6 cut(s)	198, 299, 621, 1326, 1931, 1995
Zsp2I	ATGCAT	2 cut(s)	1557, 1636

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.