Rh6DG171100

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003856
Physical Location & Seq
Forward (+)
29327701 .. 29331556
3856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG171100.1

Sequence Viewer

Length: 1479 bp
ATGTGGGATTCAGAGACTGAGTCTGTTGGAGGCAGAGATTATGGCAATGGAGTTCTTGGTTTAAGCAAGCAGAGTGTTAAGACTGATGGGTTTGAGCTAAAAGGCAATTCTTGGTATGTTGCAACTGATATCTCAAGTGACCTGTTGGTTCAAGTTGGACAAGTCAATTTTCACTTGCATAAGTATCCCCTGCTGTCAAGGAGTGGAAAGATCAACAGAATCATGTATGAATTACGAGACCCGGACTTGAGTAAGATTGTTTTGGATGACCTTCCTGGTGGACCCGAGTCTTTTGAGCTAGCTGCAAAGTTCTGCTATGGAATTGCTGTTGATCTTACAGCAGCCAATATATCAGGCCTTAGATGTGCTGCAGAGTTCCTTGAAATGACAGAAGACTTGGAAGAAGGCAATCTTATTTTCAAAACCGAAGCATTTCTCAGCTATGTGGTTTTGTCCTCGTGGAGAGACTCCATAGTAGTGTTGAAAAGTTGTGAGAAGCTCTCACCATGGGCTGAAAACCTCCAAATTGTTCGAAGATGTAGCGAGTCCATTGCTTGGAAAGCTTGTGCCAATCCAAAAGGGATACGATGGGCATACACCGGTAAGCCTCCAAAAGTTTCAAGCCCAACTTGGAATGACATGAAAGACTCAAGTCCAAGTAGAAACCAGCAAGTTCCTCCTGATTGGTGGTTTGAAGATGCTTCAATTCTTCGCATTGACCATTACGTCAGGGTTATTACTGCTATTAAGGTAAAGGGGATGAGATTTGAACTGATTGGAGCTGCAATAATGCACTATGCAGCTAAATGGCTCCCTGGTATGATAACTGATGGCTCGGGGGCAGCCGATGAAGGAAGCAACACCAGCAATAGTAATACTGGTAATACCGGGAGCAGATGGAAGGGTGGACTCCACCTGATTGTGGCTGGAACTAAAGATGAGCCTTCACCTGTTCAGGCCAAAGATCAACGTATGATTATTGAGAGCCTTATTAGCATTATTCCTCCACAGAAGGATAGTGTTTCATGCAGCTTCCTTCTTCGACTTTTGAGAATGGCAAACATGTTGAAAGTAGCACCTGCACTGGTTACAGAGTTGGAAAAACGGGTGGGAATGCAGTTCGAACAGGCTACATTGGCAGATCTTCTTATTCCTTCTTTCAATAAGAATGAAACTATGTATGATGTGGATCTTGTTCAGAGGCTTCTGGAGCATTTTCTTGTTCAAGAACAGACTGAAATTTCAAGTCCAAGCCAACAATCTTTTATTGAAAAACACTACGATGGAACTCAAAGGGGTACAGGCCCAAATGCTAAGATGAGGGTAGCTAGGCTTGTTGATAGTTATCTTACAGAGGTGTCAAGAGATAGAAGTCTTTCCCTAACAAAGTTTCAGGTACTGGCAGAAGCTTTGCCTGAATCTGCAAGAGTATGTGATGATGGACTTTATAGAGCAATTGATTCATATCTCAAGGTATAA

Protein Analysis

492

Amino Acids

54.82

Weight (kDa)

5.69

Isoelectric Point (pI)

48.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 228 - 491 8.3e-81 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1087
AasI GACNNNNNNGTC 1 cut(s) 725
Acc36I ACCTGC 1 cut(s) 1087
AccB7I CCANNNNNTGG 1 cut(s) 555
AclWI GGATC 1 cut(s) 1197
AcsI RAATTY 1 cut(s) 1239
AfaI GTAC 2 cut(s) 1300, 1398
AfiI CCNNNNNNNGG 2 cut(s) 555, 922
AflIII ACRYGT 1 cut(s) 1062
AgeI ACCGGT 1 cut(s) 599
AjnI CCWGG 2 cut(s) 274, 814
Alw26I GTCTC 3 cut(s) 8, 231, 459
AlwI GGATC 1 cut(s) 1197
AlwNI CAGNNNCTG 2 cut(s) 17, 1399
Ama87I CYCGRG 2 cut(s) 284, 835
AoxI GGCC 3 cut(s) 355, 957, 1303
ApeKI GCWGC 7 cut(s) 302, 341, 368, 782, 800, 842, 1029
ApoI RAATTY 1 cut(s) 1239
AsiGI ACCGGT 1 cut(s) 599
Asp700I GAANNNNTTC 2 cut(s) 432, 1375
AspS9I GGNCC 2 cut(s) 281, 1304
AsuC2I CCSGG 2 cut(s) 242, 889
AsuHPI GGTGA 2 cut(s) 495, 939
AsuII TTCGAA 2 cut(s) 532, 1122
AsuNHI GCTAGC 1 cut(s) 298
AvaI CYCGRG 2 cut(s) 284, 835
AvaII GGWCC 1 cut(s) 281
BauI CACGAG 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 399
BbvI GCAGC 7 cut(s) 289, 353, 355, 769, 812, 854, 1041
BccI CCATC 6 cut(s) 80, 582, 824, 891, 1277, 1433
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 2 cut(s) 276, 816
BciVI GTATCC 2 cut(s) 195, 576
BcnI CCSGG 2 cut(s) 242, 889
BcoDI GTCTC 3 cut(s) 8, 231, 459
BfaI CTAG 2 cut(s) 299, 1329
BfmI CTRYAG 1 cut(s) 369
BfuAI ACCTGC 1 cut(s) 1087
BfuI GTATCC 2 cut(s) 195, 576
BglII AGATCT 1 cut(s) 1141
BisI GCNGC 7 cut(s) 303, 342, 369, 783, 801, 843, 1030
BlsI GCNGC 7 cut(s) 304, 343, 370, 784, 802, 844, 1031
Bme1390I CCNGG 4 cut(s) 242, 276, 816, 889
Bme18I GGWCC 1 cut(s) 281
BmeT110I CYCGRG 2 cut(s) 284, 835
BmgT120I GGNCC 2 cut(s) 281, 1304
BmiI GGNNCC 2 cut(s) 283, 812
BmrFI CCNGG 4 cut(s) 242, 276, 816, 889
BmsI GCATC 1 cut(s) 688
BmtI GCTAGC 1 cut(s) 302
BoxI GACNNNNGTC 2 cut(s) 286, 651
BpiI GAAGAC 1 cut(s) 399
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 1 cut(s) 1229
Bpu14I TTCGAA 2 cut(s) 532, 1122
BpuEI CTTGAG 4 cut(s) 118, 268, 634, 1454
BpuMI CCSGG 2 cut(s) 242, 889
BsaBI GATNNNNATC 3 cut(s) 1188, 1344, 1464
BsaI GGTCTC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 506, 814
BsaWI WCCGGW 1 cut(s) 599
Bsc4I CCNNNNNNNGG 2 cut(s) 555, 922
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 3 cut(s) 883, 1089, 1404
Bse3DI GCAATG 2 cut(s) 52, 549
Bse8I GATNNNNATC 3 cut(s) 1188, 1344, 1464
BseBI CCWGG 2 cut(s) 276, 816
BseDI CCNNGG 2 cut(s) 506, 814
BseGI GGATG 2 cut(s) 271, 765
BseJI GATNNNNATC 3 cut(s) 1188, 1344, 1464
BseLI CCNNNNNNNGG 2 cut(s) 555, 922
BseMI GCAATG 2 cut(s) 52, 549
BseMII CTCAG 2 cut(s) 9, 451
BseNI ACTGG 3 cut(s) 883, 1089, 1404
BseXI GCAGC 7 cut(s) 289, 353, 355, 769, 812, 854, 1041
BsgI GTGCAG 1 cut(s) 1065
BshFI GGCC 3 cut(s) 357, 959, 1305
BshTI ACCGGT 1 cut(s) 599
BsiHKCI CYCGRG 2 cut(s) 284, 835
BsiSI CCGG 3 cut(s) 242, 600, 888
BslI CCNNNNNNNGG 2 cut(s) 555, 922
BsmAI GTCTC 3 cut(s) 8, 231, 459
BsmI GAATGC 1 cut(s) 1119
BsnI GGCC 3 cut(s) 357, 959, 1305
Bso31I GGTCTC 1 cut(s) 231
BsoBI CYCGRG 2 cut(s) 284, 835
Bsp119I TTCGAA 2 cut(s) 532, 1122
Bsp143I GATC 5 cut(s) 210, 331, 964, 1141, 1189
Bsp19I CCATGG 1 cut(s) 506
BspANI GGCC 3 cut(s) 357, 959, 1305
BspCNI CTCAG 2 cut(s) 10, 450
BspLI GGNNCC 2 cut(s) 283, 812
BspMAI CTGCAG 1 cut(s) 373
BspMI ACCTGC 1 cut(s) 1087
BspOI GCTAGC 1 cut(s) 302
BspPI GGATC 1 cut(s) 1197
BspT104I TTCGAA 2 cut(s) 532, 1122
BspTNI GGTCTC 1 cut(s) 231
BsrDI GCAATG 2 cut(s) 52, 549
BsrFI RCCGGY 1 cut(s) 599
BsrI ACTGG 3 cut(s) 883, 1089, 1404
BssAI RCCGGY 1 cut(s) 599
BssECI CCNNGG 2 cut(s) 506, 814
BssMI GATC 5 cut(s) 210, 331, 964, 1141, 1189
BssSI CACGAG 1 cut(s) 457
BssT1I CCWWGG 1 cut(s) 506
Bst2BI CACGAG 1 cut(s) 457
Bst2UI CCWGG 2 cut(s) 276, 816
BstBI TTCGAA 2 cut(s) 532, 1122
BstC8I GCNNGC 2 cut(s) 68, 300
BstDEI CTNAG 4 cut(s) 18, 359, 437, 1314
BstDSI CCRYGG 1 cut(s) 506
BstF5I GGATG 2 cut(s) 271, 765
BstKTI GATC 5 cut(s) 213, 334, 967, 1144, 1192
BstMAI GTCTC 3 cut(s) 8, 231, 459
BstMBI GATC 5 cut(s) 210, 331, 964, 1141, 1189
BstMWI GCNNNNNNNGC 5 cut(s) 560, 864, 993, 1136, 1210
BstNI CCWGG 2 cut(s) 276, 816
BstNSI RCATGY 1 cut(s) 1066
BstPAI GACNNNNGTC 2 cut(s) 286, 651
BstSCI CCNGG 4 cut(s) 240, 274, 814, 887
BstSFI CTRYAG 1 cut(s) 369
BstV1I GCAGC 7 cut(s) 289, 353, 355, 769, 812, 854, 1041
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 2 cut(s) 1141, 1189
BstYI RGATCY 2 cut(s) 1141, 1189
BsuI GTATCC 2 cut(s) 195, 576
BsuRI GGCC 3 cut(s) 357, 959, 1305
BtgI CCRYGG 1 cut(s) 506
BtsCI GGATG 2 cut(s) 271, 765
BtsIMutI CAGTG 1 cut(s) 1082
BveI ACCTGC 1 cut(s) 1087
Cac8I GCNNGC 2 cut(s) 68, 300
CaiI CAGNNNCTG 2 cut(s) 17, 1399
Cfr10I RCCGGY 1 cut(s) 599
Cfr13I GGNCC 2 cut(s) 281, 1304
Csp6I GTAC 2 cut(s) 1299, 1397
CspAI ACCGGT 1 cut(s) 599
CviAII CATG 5 cut(s) 223, 507, 640, 1026, 1063
CviQI GTAC 2 cut(s) 1299, 1397
DdeI CTNAG 4 cut(s) 18, 359, 437, 1314
DpnI GATC 5 cut(s) 212, 333, 966, 1143, 1191
DpnII GATC 5 cut(s) 210, 331, 964, 1141, 1189
DrdI GACNNNNNNGTC 1 cut(s) 725
DseDI GACNNNNNNGTC 1 cut(s) 725
Eco130I CCWWGG 1 cut(s) 506
Eco147I AGGCCT 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 231
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 281
Eco88I CYCGRG 2 cut(s) 284, 835
EcoRII CCWGG 2 cut(s) 274, 814
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 506
ErhI CCWWGG 1 cut(s) 506
FaeI CATG 5 cut(s) 226, 510, 643, 1029, 1066
FalI AAGNNNNNCTT 4 cut(s) 396, 428, 613, 645
FatI CATG 5 cut(s) 222, 506, 639, 1025, 1062
Fnu4HI GCNGC 7 cut(s) 303, 342, 369, 783, 801, 843, 1030
FokI GGATG 2 cut(s) 278, 772
Fsp4HI GCNGC 7 cut(s) 303, 342, 369, 783, 801, 843, 1030
FspBI CTAG 2 cut(s) 299, 1329
GluI GCNGC 7 cut(s) 303, 342, 369, 783, 801, 843, 1030
GsuI CTGGAG 1 cut(s) 1229
HaeIII GGCC 3 cut(s) 357, 959, 1305
HapII CCGG 3 cut(s) 242, 600, 888
Hin1II CATG 5 cut(s) 226, 510, 643, 1029, 1066
HindIII AAGCTT 2 cut(s) 561, 1407
HpaII CCGG 3 cut(s) 242, 600, 888
HphI GGTGA 2 cut(s) 495, 939
Hpy166II GTNNAC 2 cut(s) 281, 908
Hpy188I TCNGA 2 cut(s) 13, 1200
Hpy188III TCNNGA 4 cut(s) 680, 1208, 1226, 1362
Hpy8I GTNNAC 2 cut(s) 281, 908
HpyAV CCTTC 8 cut(s) 281, 398, 845, 895, 954, 1006, 1046, 1164
HpyCH4IV ACGT 2 cut(s) 726, 970
HpyF10VI GCNNNNNNNGC 5 cut(s) 560, 864, 993, 1136, 1210
HpyF3I CTNAG 4 cut(s) 18, 359, 437, 1314
HpySE526I ACGT 2 cut(s) 726, 970
Hsp92II CATG 5 cut(s) 226, 510, 643, 1029, 1066
Kzo9I GATC 5 cut(s) 210, 331, 964, 1141, 1189
LmnI GCTCC 4 cut(s) 779, 816, 891, 1210
Lsp1109I GCAGC 7 cut(s) 289, 353, 355, 769, 812, 854, 1041
LweI GCATC 1 cut(s) 688
MaeI CTAG 2 cut(s) 299, 1329
MaeII ACGT 2 cut(s) 726, 970
MaeIII GTNAC 2 cut(s) 137, 1087
MalI GATC 5 cut(s) 212, 333, 966, 1143, 1191
MboI GATC 5 cut(s) 210, 331, 964, 1141, 1189
MboII GAAGA 7 cut(s) 404, 413, 546, 701, 707, 1031, 1136
MfeI CAATTG 1 cut(s) 1455
MflI RGATCY 2 cut(s) 1141, 1189
MluCI AATT 8 cut(s) 106, 166, 230, 321, 525, 705, 1239, 1455
MlyI GAGTC 6 cut(s) 29, 296, 461, 554, 641, 903
MmeI TCCRAC 3 cut(s) 7, 136, 1077
MnlI CCTC 9 cut(s) 23, 466, 530, 618, 687, 1014, 1194, 1314, 1348
MroXI GAANNNNTTC 2 cut(s) 432, 1375
MseI TTAA 3 cut(s) 62, 78, 747
MslI CAYNNNNRTG 2 cut(s) 476, 1281
MspI CCGG 3 cut(s) 242, 600, 888
MspR9I CCNGG 4 cut(s) 242, 276, 816, 889
MunI CAATTG 1 cut(s) 1455
Mva1269I GAATGC 1 cut(s) 1119
MvaI CCWGG 2 cut(s) 276, 816
MwoI GCNNNNNNNGC 5 cut(s) 560, 864, 993, 1136, 1210
NciI CCSGG 2 cut(s) 242, 889
NcoI CCATGG 1 cut(s) 506
NdeII GATC 5 cut(s) 210, 331, 964, 1141, 1189
NheI GCTAGC 1 cut(s) 298
NlaIII CATG 5 cut(s) 226, 510, 643, 1029, 1066
NlaIV GGNNCC 2 cut(s) 283, 812
NmuCI GTSAC 1 cut(s) 137
NspI RCATGY 1 cut(s) 1066
NspV TTCGAA 2 cut(s) 532, 1122
PaqCI CACCTGC 1 cut(s) 1087
PceI AGGCCT 1 cut(s) 357
PciI ACATGT 1 cut(s) 1062
PctI GAATGC 1 cut(s) 1119
PdmI GAANNNNTTC 2 cut(s) 432, 1375
PfeI GAWTC 4 cut(s) 8, 219, 1418, 1460
PflFI GACNNNGTC 1 cut(s) 19
PflMI CCANNNNNTGG 1 cut(s) 555
PinAI ACCGGT 1 cut(s) 599
PkrI GCNGC 7 cut(s) 304, 343, 370, 784, 802, 844, 1031
PleI GAGTC 6 cut(s) 28, 295, 461, 553, 641, 903
PpsI GAGTC 6 cut(s) 28, 295, 461, 553, 641, 903
PscI ACATGT 1 cut(s) 1062
PshAI GACNNNNGTC 2 cut(s) 286, 651
Psp6I CCWGG 2 cut(s) 274, 814
PspGI CCWGG 2 cut(s) 274, 814
PspN4I GGNNCC 2 cut(s) 283, 812
PspPI GGNCC 2 cut(s) 281, 1304
PstI CTGCAG 1 cut(s) 373
PstNI CAGNNNCTG 2 cut(s) 17, 1399
PsuI RGATCY 2 cut(s) 1141, 1189
PsyI GACNNNGTC 1 cut(s) 19
RsaI GTAC 2 cut(s) 1300, 1398
RsaNI GTAC 2 cut(s) 1299, 1397
RseI CAYNNNNRTG 2 cut(s) 476, 1281
SaqAI TTAA 3 cut(s) 62, 78, 747
SatI GCNGC 7 cut(s) 303, 342, 369, 783, 801, 843, 1030
Sau3AI GATC 5 cut(s) 210, 331, 964, 1141, 1189
Sau96I GGNCC 2 cut(s) 281, 1304
SchI GAGTC 6 cut(s) 29, 296, 461, 554, 641, 903
ScrFI CCNGG 4 cut(s) 242, 276, 816, 889
SfaNI GCATC 1 cut(s) 688
SfcI CTRYAG 1 cut(s) 369
SfuI TTCGAA 2 cut(s) 532, 1122
SinI GGWCC 1 cut(s) 281
SmiMI CAYNNNNRTG 2 cut(s) 476, 1281
SmlI CTYRAG 4 cut(s) 133, 247, 649, 1469
SmoI CTYRAG 4 cut(s) 133, 247, 649, 1469
Sse9I AATT 8 cut(s) 106, 166, 230, 321, 525, 705, 1239, 1455
SseBI AGGCCT 1 cut(s) 357
SspMI CTAG 2 cut(s) 299, 1329
StuI AGGCCT 1 cut(s) 357
StyD4I CCNGG 4 cut(s) 240, 274, 814, 887
StyI CCWWGG 1 cut(s) 506
TaiI ACGT 2 cut(s) 729, 973
TaqI TCGA 3 cut(s) 532, 1042, 1122
TasI AATT 8 cut(s) 106, 166, 230, 321, 525, 705, 1239, 1455
TfiI GAWTC 4 cut(s) 8, 219, 1418, 1460
Tru1I TTAA 3 cut(s) 62, 78, 747
Tru9I TTAA 3 cut(s) 62, 78, 747
TscAI CASTG 1 cut(s) 1089
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 7 cut(s) 302, 341, 368, 782, 800, 842, 1029
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 6 cut(s) 243, 656, 864, 1014, 1185, 1452
TspRI CASTG 1 cut(s) 1089
Tth111I GACNNNGTC 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 555
VpaK11BI GGWCC 1 cut(s) 281
XapI RAATTY 1 cut(s) 1239
XceI RCATGY 1 cut(s) 1066
XmnI GAANNNNTTC 2 cut(s) 432, 1375
XspI CTAG 2 cut(s) 299, 1329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.