RchiOBHm_Chr7g0216061

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003856
Physical Location & Seq
Forward (+)
33724655 .. 33726954
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19332

Sequence Viewer

Length: 1647 bp
ATGACAGAGGACTTGGAAGAAGGCAATCTTATTTTCAAAACCGAAGCATTTCTCAGCTATGTGGTTTTGTCCTCATGGAGAGACTCCATATTAGTGTTGAAAAGTTGCGAGAAGCTCTCACCGTGGGCTGAAAACCTTCAAATTGTTCGGAGATGCAGCGAGTCTATTGCTTGGAAAGCTTGTGCCAATCCAAAAGGGATAAGATGGCCATATACTGGTAGTAAGCTAGCTCCAAAAGTTTCAAGCCCAAATTGGAGTGACGTGAAGGACTCAAGTCCAAGTGGAAACCAGCTGCAAGTTCCTTCTGATTGGTGGTTTGAAGATGCTTCAATTCTTCGGATTGATCACTATGTCAAGGTTATTACCGCAATCAAGGTGAAGGGGATGAGATATGAACTGCTTGGAGCTGCAATAATGCACTATGCAGCTAAATGGCTCCCTATTATGATAAGTGAGCATGGCTCTGGGTTAACAGCTGATGAAGGAAGCAACAGCAGCAGTAGCAGTGGAAGCAGTTGGAAGGGTGGACTTCACATGATCGTGGCAGGAACTAAAGATAATGAGTCTTCAGCTGTTCAGGCCAAAGGTCAACGGATGATCATTGAGAGCCTTATTAGCATAATTCCACCACAGAAGGACAGTGTCTCATGTAGCTTCCTTCTTCGGCTTCTGAGAATGGCAAACATGTTGAAAGTAGCACCTGCACTGGTTACTGAGTTGGAGAAACGGGTGGGAATGCAGTTCGAACAGGCTACATTGGCGGATCTTCTCATTCCGTCTTGCAACAAAAGTGAAAGTATGTATGATGTGGATCTTGTCCAGAGGCTTCTGGAGCATTTTCTTGTTCAAGAACAGACTGAAATGTCAAGTCCAAGCCACCAATCTTTCATTGAAAAACACTATGAAAGGGGCACAGGCCCAAATGCTAAGATGAGGGTAGCCAAGCTTGTTGATAGTTATCTTACAGAGGTGTCAAGAGATAGAAGCCTTTCCCTAACAAAGTTTCAGGTACTCGCAGAAGCTTTGCCTGAATCTGCAAGGACCTGTGATGATGGACTTTATAGAGCAATTGATTCCTATCTCAAGGCTCATCCAACACTCTCTGAGCACGAAAGAAAGAGGCTATGCCGGGTAATGGATTGCCAAAAGCTATCGATTGATGCTTGCATGCATGCTGCACAAAATGAAAGGCTCCCATTAAGAGTGGTGGTGCAAGTTCTTTTCTCTGAGCAAGTCAAAATAAGCAATGCATTGGCCAACAATGCCCTTAATGAAGCCGGAGGAGACTCTAACTATCAGCCAATGATTCCAAATCGGAAAACACTACTCGAAGGGACCCCGCAATCCTTCCAAGAAGGATGGGCAGCAGCTAAAAAGGACATTAACACTGTAAAGTTTGAACTGGAGAGTGTAAAAGCAAAGTACCTTGATCTCCAAAATTCCATGGAAAACTTGCAGAGACAGTTCGATAAGATGACCTCAAAGCAGAAACATACATCAGCTTGGAGCAGCGGATGGAAGAAACTCGGCAAACTCACTAAGACTACAATGTTCGAGAATCAGAACAGTGGACCGGAGACTCCAACAGCGGCTGGTCCGGAGCAGACAAGAAAGACTCCTAGGAGGTGGAGGAATTCCATTTCTTAG

Protein Analysis

548

Amino Acids

61.43

Weight (kDa)

8.75

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 103 - 389 4.7e-99 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 709
AasI GACNNNNNNGTC 1 cut(s) 862
Acc36I ACCTGC 1 cut(s) 709
AccB7I CCANNNNNTGG 1 cut(s) 215
AccIII TCCGGA 1 cut(s) 1597
AciI CCGC 5 cut(s) 366, 761, 1340, 1512, 1589
AclWI GGATC 2 cut(s) 771, 819
AcoI YGGCCR 2 cut(s) 206, 1254
AcsI RAATTY 2 cut(s) 1438, 1633
AcuI CTGAAG 1 cut(s) 552
AfaI GTAC 2 cut(s) 1011, 1424
AfiI CCNNNNNNNGG 2 cut(s) 215, 1135
AflIII ACRYGT 1 cut(s) 684
AjiI CACGTC 1 cut(s) 262
Alw21I GWGCWC 1 cut(s) 1110
Alw26I GTCTC 5 cut(s) 75, 649, 1278, 1453, 1571
AlwI GGATC 2 cut(s) 771, 819
AlwNI CAGNNNCTG 1 cut(s) 1592
Aor13HI TCCGGA 1 cut(s) 1597
AoxI GGCC 4 cut(s) 206, 579, 916, 1254
ApeKI GCWGC 9 cut(s) 156, 292, 407, 425, 495, 1175, 1364, 1367, 1509
ApoI RAATTY 2 cut(s) 1438, 1633
Asp700I GAANNNNTTC 3 cut(s) 48, 135, 988
AspA2I CCTAGG 1 cut(s) 1619
AspS9I GGNCC 5 cut(s) 917, 1041, 1335, 1571, 1595
AsuC2I CCSGG 1 cut(s) 1130
AsuHPI GGTGA 2 cut(s) 111, 388
AsuII TTCGAA 1 cut(s) 744
AsuNHI GCTAGC 1 cut(s) 226
AvaII GGWCC 4 cut(s) 1041, 1335, 1571, 1595
AvrII CCTAGG 1 cut(s) 1619
BaeGI GKGCMC 1 cut(s) 914
BalI TGGCCA 2 cut(s) 208, 1256
BbsI GAAGAC 1 cut(s) 558
Bbv12I GWGCWC 1 cut(s) 1110
BbvI GCAGC 9 cut(s) 168, 279, 394, 437, 507, 1162, 1376, 1379, 1521
BccI CCATC 4 cut(s) 198, 1046, 1353, 1509
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BclI TGATCA 2 cut(s) 343, 597
BcnI CCSGG 1 cut(s) 1130
BcoDI GTCTC 5 cut(s) 75, 649, 1278, 1453, 1571
BfaI CTAG 2 cut(s) 227, 1620
BfuAI ACCTGC 1 cut(s) 709
BlnI CCTAGG 1 cut(s) 1619
Bme1390I CCNGG 1 cut(s) 1130
Bme18I GGWCC 4 cut(s) 1041, 1335, 1571, 1595
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 5 cut(s) 917, 1041, 1335, 1571, 1595
BmiI GGNNCC 4 cut(s) 437, 1193, 1336, 1337
BmrFI CCNGG 1 cut(s) 1130
BmsI GCATC 3 cut(s) 143, 313, 1150
BmtI GCTAGC 1 cut(s) 230
BoxI GACNNNNGTC 1 cut(s) 273
BpiI GAAGAC 1 cut(s) 558
BplI GAGNNNNNCTC 4 cut(s) 101, 133, 446, 478
BpmI CTGGAG 2 cut(s) 851, 1424
Bpu14I TTCGAA 1 cut(s) 744
BpuEI CTTGAG 2 cut(s) 256, 1067
BpuMI CCSGG 1 cut(s) 1130
Bsa29I ATCGAT 1 cut(s) 1154
BsaBI GATNNNNATC 3 cut(s) 810, 957, 1077
BsaJI CCNNGG 3 cut(s) 122, 1443, 1619
BsaWI WCCGGW 2 cut(s) 1573, 1597
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 1135
Bse1I ACTGG 3 cut(s) 220, 711, 1407
Bse3DI GCAATG 1 cut(s) 1252
Bse8I GATNNNNATC 3 cut(s) 810, 957, 1077
BseAI TCCGGA 1 cut(s) 1597
BseCI ATCGAT 1 cut(s) 1154
BseDI CCNNGG 3 cut(s) 122, 1443, 1619
BseGI GGATG 5 cut(s) 390, 600, 1090, 1364, 1520
BseJI GATNNNNATC 3 cut(s) 810, 957, 1077
BseLI CCNNNNNNNGG 2 cut(s) 215, 1135
BseMI GCAATG 1 cut(s) 1252
BseMII CTCAG 5 cut(s) 67, 662, 705, 1095, 1218
BseNI ACTGG 3 cut(s) 220, 711, 1407
BseRI GAGGAG 1 cut(s) 1296
BseSI GKGCMC 1 cut(s) 914
BseXI GCAGC 9 cut(s) 168, 279, 394, 437, 507, 1162, 1376, 1379, 1521
BsgI GTGCAG 2 cut(s) 687, 1161
BshFI GGCC 4 cut(s) 208, 581, 918, 1256
BshVI ATCGAT 1 cut(s) 1154
BsiHKAI GWGCWC 1 cut(s) 1110
BsiSI CCGG 4 cut(s) 1129, 1278, 1574, 1598
BslFI GGGAC 1 cut(s) 1348
BslI CCNNNNNNNGG 2 cut(s) 215, 1135
BsmAI GTCTC 5 cut(s) 75, 649, 1278, 1453, 1571
BsmFI GGGAC 1 cut(s) 1348
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 4 cut(s) 208, 581, 918, 1256
Bsp119I TTCGAA 1 cut(s) 744
Bsp1286I GDGCHC 2 cut(s) 914, 1110
Bsp13I TCCGGA 1 cut(s) 1597
Bsp143I GATC 6 cut(s) 343, 537, 597, 763, 811, 1429
Bsp19I CCATGG 1 cut(s) 1443
BspACI CCGC 5 cut(s) 366, 761, 1340, 1512, 1589
BspANI GGCC 4 cut(s) 208, 581, 918, 1256
BspCNI CTCAG 5 cut(s) 66, 663, 706, 1096, 1219
BspDI ATCGAT 1 cut(s) 1154
BspEI TCCGGA 1 cut(s) 1597
BspLI GGNNCC 4 cut(s) 437, 1193, 1336, 1337
BspMI ACCTGC 1 cut(s) 709
BspOI GCTAGC 1 cut(s) 230
BspPI GGATC 2 cut(s) 771, 819
BspT104I TTCGAA 1 cut(s) 744
BsrDI GCAATG 1 cut(s) 1252
BsrI ACTGG 3 cut(s) 220, 711, 1407
BssECI CCNNGG 3 cut(s) 122, 1443, 1619
BssMI GATC 6 cut(s) 343, 537, 597, 763, 811, 1429
BssT1I CCWWGG 2 cut(s) 1443, 1619
Bst4CI ACNGT 5 cut(s) 123, 641, 1390, 1464, 1568
BstBI TTCGAA 1 cut(s) 744
BstC8I GCNNGC 4 cut(s) 228, 1165, 1169, 1173
BstDEI CTNAG 8 cut(s) 53, 671, 714, 927, 1104, 1227, 1539, 1644
BstDSI CCRYGG 2 cut(s) 122, 1443
BstF5I GGATG 5 cut(s) 390, 600, 1090, 1364, 1520
BstKTI GATC 6 cut(s) 346, 540, 600, 766, 814, 1432
BstMAI GTCTC 5 cut(s) 75, 649, 1278, 1453, 1571
BstMBI GATC 6 cut(s) 343, 537, 597, 763, 811, 1429
BstMWI GCNNNNNNNGC 9 cut(s) 176, 495, 501, 510, 578, 615, 758, 832, 1262
BstNSI RCATGY 3 cut(s) 688, 1171, 1175
BstPAI GACNNNNGTC 1 cut(s) 273
BstSCI CCNGG 1 cut(s) 1128
BstSLI GKGCMC 1 cut(s) 914
BstV1I GCAGC 9 cut(s) 168, 279, 394, 437, 507, 1162, 1376, 1379, 1521
BstV2I GAAGAC 1 cut(s) 558
BstX2I RGATCY 2 cut(s) 763, 811
BstYI RGATCY 2 cut(s) 763, 811
Bsu15I ATCGAT 1 cut(s) 1154
BsuRI GGCC 4 cut(s) 208, 581, 918, 1256
BsuTUI ATCGAT 1 cut(s) 1154
BtgI CCRYGG 2 cut(s) 122, 1443
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 5 cut(s) 390, 600, 1090, 1364, 1520
BtsI GCAGTG 1 cut(s) 511
BtsIMutI CAGTG 5 cut(s) 511, 646, 704, 1386, 1573
BveI ACCTGC 1 cut(s) 709
Cac8I GCNNGC 4 cut(s) 228, 1165, 1169, 1173
CaiI CAGNNNCTG 1 cut(s) 1592
Cfr13I GGNCC 5 cut(s) 917, 1041, 1335, 1571, 1595
ClaI ATCGAT 1 cut(s) 1154
Csp6I GTAC 2 cut(s) 1010, 1423
CviAII CATG 8 cut(s) 75, 458, 535, 648, 685, 1168, 1172, 1444
CviQI GTAC 2 cut(s) 1010, 1423
DdeI CTNAG 8 cut(s) 53, 671, 714, 927, 1104, 1227, 1539, 1644
DpnI GATC 6 cut(s) 345, 539, 599, 765, 813, 1431
DpnII GATC 6 cut(s) 343, 537, 597, 763, 811, 1429
DrdI GACNNNNNNGTC 1 cut(s) 862
DseDI GACNNNNNNGTC 1 cut(s) 862
EaeI YGGCCR 2 cut(s) 206, 1254
EciI GGCGGA 1 cut(s) 776
Eco130I CCWWGG 2 cut(s) 1443, 1619
Eco47I GGWCC 4 cut(s) 1041, 1335, 1571, 1595
Eco57I CTGAAG 1 cut(s) 552
EcoO109I RGGNCCY 2 cut(s) 1041, 1335
EcoRI GAATTC 1 cut(s) 1633
EcoT14I CCWWGG 2 cut(s) 1443, 1619
EcoT22I ATGCAT 2 cut(s) 1173, 1252
ErhI CCWWGG 2 cut(s) 1443, 1619
FaeI CATG 8 cut(s) 78, 461, 538, 651, 688, 1171, 1175, 1447
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FaqI GGGAC 1 cut(s) 1348
FatI CATG 8 cut(s) 74, 457, 534, 647, 684, 1167, 1171, 1443
FauI CCCGC 1 cut(s) 1347
FbaI TGATCA 2 cut(s) 343, 597
FokI GGATG 5 cut(s) 397, 607, 1077, 1371, 1527
FspBI CTAG 2 cut(s) 227, 1620
GsuI CTGGAG 2 cut(s) 851, 1424
HaeIII GGCC 4 cut(s) 208, 581, 918, 1256
HapII CCGG 4 cut(s) 1129, 1278, 1574, 1598
Hin1II CATG 8 cut(s) 78, 461, 538, 651, 688, 1171, 1175, 1447
HincII GTYRAC 2 cut(s) 471, 590
HindII GTYRAC 2 cut(s) 471, 590
HindIII AAGCTT 3 cut(s) 177, 944, 1020
HpaI GTTAAC 1 cut(s) 471
HpaII CCGG 4 cut(s) 1129, 1278, 1574, 1598
HphI GGTGA 2 cut(s) 111, 388
Hpy166II GTNNAC 4 cut(s) 471, 527, 590, 1571
Hpy188I TCNGA 8 cut(s) 150, 307, 339, 672, 1105, 1228, 1317, 1563
Hpy188III TCNNGA 6 cut(s) 820, 830, 848, 975, 1555, 1598
Hpy8I GTNNAC 4 cut(s) 471, 527, 590, 1571
HpyCH4III ACNGT 5 cut(s) 123, 641, 1390, 1464, 1568
HpyCH4IV ACGT 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 9 cut(s) 176, 495, 501, 510, 578, 615, 758, 832, 1262
HpyF3I CTNAG 8 cut(s) 53, 671, 714, 927, 1104, 1227, 1539, 1644
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 8 cut(s) 78, 461, 538, 651, 688, 1171, 1175, 1447
KflI GGGWCCC 1 cut(s) 1335
Kpn2I TCCGGA 1 cut(s) 1597
Ksp22I TGATCA 2 cut(s) 343, 597
KspAI GTTAAC 1 cut(s) 471
Kzo9I GATC 6 cut(s) 343, 537, 597, 763, 811, 1429
LmnI GCTCC 7 cut(s) 235, 404, 441, 832, 1197, 1506, 1600
Lsp1109I GCAGC 9 cut(s) 168, 279, 394, 437, 507, 1162, 1376, 1379, 1521
LweI GCATC 3 cut(s) 143, 313, 1150
MaeI CTAG 2 cut(s) 227, 1620
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 257, 709
MalI GATC 6 cut(s) 345, 539, 599, 765, 813, 1431
MboI GATC 6 cut(s) 343, 537, 597, 763, 811, 1429
MboII GAAGA 7 cut(s) 29, 326, 332, 558, 653, 758, 1531
MfeI CAATTG 1 cut(s) 1068
MflI RGATCY 2 cut(s) 763, 811
MhlI GDGCHC 2 cut(s) 914, 1110
MlsI TGGCCA 2 cut(s) 208, 1256
MluCI AATT 7 cut(s) 141, 250, 330, 621, 1068, 1438, 1633
MluNI TGGCCA 2 cut(s) 208, 1256
MlyI GAGTC 7 cut(s) 77, 170, 263, 572, 1280, 1573, 1609
MmeI TCCRAC 4 cut(s) 497, 699, 1118, 1607
MnlI CCTC 9 cut(s) 82, 816, 927, 961, 1113, 1274, 1489, 1617, 1623
Mox20I TGGCCA 2 cut(s) 208, 1256
Mph1103I ATGCAT 2 cut(s) 1173, 1252
MroI TCCGGA 1 cut(s) 1597
MroXI GAANNNNTTC 3 cut(s) 48, 135, 988
MscI TGGCCA 2 cut(s) 208, 1256
MseI TTAA 4 cut(s) 470, 1199, 1269, 1383
MslI CAYNNNNRTG 2 cut(s) 92, 539
Msp20I TGGCCA 2 cut(s) 208, 1256
MspA1I CMGCKG 5 cut(s) 292, 476, 572, 1512, 1589
MspI CCGG 4 cut(s) 1129, 1278, 1574, 1598
MspR9I CCNGG 1 cut(s) 1130
MunI CAATTG 1 cut(s) 1068
Mva1269I GAATGC 1 cut(s) 741
MwoI GCNNNNNNNGC 9 cut(s) 176, 495, 501, 510, 578, 615, 758, 832, 1262
NciI CCSGG 1 cut(s) 1130
NcoI CCATGG 1 cut(s) 1443
NdeII GATC 6 cut(s) 343, 537, 597, 763, 811, 1429
NheI GCTAGC 1 cut(s) 226
NlaIII CATG 8 cut(s) 78, 461, 538, 651, 688, 1171, 1175, 1447
NlaIV GGNNCC 4 cut(s) 437, 1193, 1336, 1337
NmeAIII GCCGAG 1 cut(s) 1506
NmuCI GTSAC 1 cut(s) 257
NsiI ATGCAT 2 cut(s) 1173, 1252
NspI RCATGY 3 cut(s) 688, 1171, 1175
NspV TTCGAA 1 cut(s) 744
PaeI GCATGC 2 cut(s) 1171, 1175
PaqCI CACCTGC 1 cut(s) 709
PciI ACATGT 1 cut(s) 684
PctI GAATGC 1 cut(s) 741
PdmI GAANNNNTTC 3 cut(s) 48, 135, 988
PfeI GAWTC 4 cut(s) 1031, 1073, 1306, 1558
PflFI GACNNNGTC 1 cut(s) 641
PflMI CCANNNNNTGG 1 cut(s) 215
PleI GAGTC 7 cut(s) 77, 169, 263, 571, 1280, 1573, 1609
PpsI GAGTC 7 cut(s) 77, 169, 263, 571, 1280, 1573, 1609
PpuMI RGGWCCY 2 cut(s) 1041, 1335
PscI ACATGT 1 cut(s) 684
PshAI GACNNNNGTC 1 cut(s) 273
Psp5II RGGWCCY 2 cut(s) 1041, 1335
PspN4I GGNNCC 4 cut(s) 437, 1193, 1336, 1337
PspPI GGNCC 5 cut(s) 917, 1041, 1335, 1571, 1595
PspPPI RGGWCCY 2 cut(s) 1041, 1335
PstNI CAGNNNCTG 1 cut(s) 1592
PsuI RGATCY 2 cut(s) 763, 811
PsyI GACNNNGTC 1 cut(s) 641
PvuII CAGCTG 3 cut(s) 292, 476, 572
RsaI GTAC 2 cut(s) 1011, 1424
RsaNI GTAC 2 cut(s) 1010, 1423
RseI CAYNNNNRTG 2 cut(s) 92, 539
SaqAI TTAA 4 cut(s) 470, 1199, 1269, 1383
Sau3AI GATC 6 cut(s) 343, 537, 597, 763, 811, 1429
Sau96I GGNCC 5 cut(s) 917, 1041, 1335, 1571, 1595
SchI GAGTC 7 cut(s) 77, 170, 263, 572, 1280, 1573, 1609
ScrFI CCNGG 1 cut(s) 1130
SduI GDGCHC 2 cut(s) 914, 1110
SfaNI GCATC 3 cut(s) 143, 313, 1150
SfuI TTCGAA 1 cut(s) 744
SinI GGWCC 4 cut(s) 1041, 1335, 1571, 1595
SmiMI CAYNNNNRTG 2 cut(s) 92, 539
SmlI CTYRAG 2 cut(s) 271, 1082
SmoI CTYRAG 2 cut(s) 271, 1082
SphI GCATGC 2 cut(s) 1171, 1175
Sse9I AATT 7 cut(s) 141, 250, 330, 621, 1068, 1438, 1633
SsiI CCGC 5 cut(s) 366, 761, 1340, 1512, 1589
SspMI CTAG 2 cut(s) 227, 1620
StyD4I CCNGG 1 cut(s) 1128
StyI CCWWGG 2 cut(s) 1443, 1619
TaaI ACNGT 5 cut(s) 123, 641, 1390, 1464, 1568
TaiI ACGT 1 cut(s) 264
TaqI TCGA 5 cut(s) 744, 1154, 1329, 1467, 1554
TasI AATT 7 cut(s) 141, 250, 330, 621, 1068, 1438, 1633
TauI GCSGC 1 cut(s) 1592
TfiI GAWTC 4 cut(s) 1031, 1073, 1306, 1558
Tru1I TTAA 4 cut(s) 470, 1199, 1269, 1383
Tru9I TTAA 4 cut(s) 470, 1199, 1269, 1383
TscAI CASTG 5 cut(s) 511, 646, 711, 1393, 1573
TseFI GTSAC 1 cut(s) 257
TseI GCWGC 9 cut(s) 156, 292, 407, 425, 495, 1175, 1364, 1367, 1509
Tsp45I GTSAC 1 cut(s) 257
TspDTI ATGAA 6 cut(s) 408, 495, 877, 918, 1200, 1287
TspGWI ACGGA 2 cut(s) 607, 765
TspRI CASTG 5 cut(s) 511, 646, 711, 1393, 1573
Tth111I GACNNNGTC 1 cut(s) 641
Van91I CCANNNNNTGG 1 cut(s) 215
VpaK11BI GGWCC 4 cut(s) 1041, 1335, 1571, 1595
XapI RAATTY 2 cut(s) 1438, 1633
XceI RCATGY 3 cut(s) 688, 1171, 1175
XmaJI CCTAGG 1 cut(s) 1619
XmnI GAANNNNTTC 3 cut(s) 48, 135, 988
XspI CTAG 2 cut(s) 227, 1620
Zsp2I ATGCAT 2 cut(s) 1173, 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.