MD10G1174900.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011288
Physical Location & Seq
Reverse (-)
26724630 .. 26728227
3598 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1174900.v1.1.491

Sequence Viewer

Length: 1887 bp
ATGGCGGACGACGAGGCAGAGATGTACGACGGAATCAGAGCTGCTTTTCCTCTATCGTTCGGGAAGCAATCTAAGTCTCAAACCCCTCTCGAAGCCATTCACAACACGACTCGTCGTTCCGTCAGCAACAACCCTCCGGAAAAATCCTCCTCCGCCGCCTCCAAAACGACCGGCCTCCCTTCTCTCTCCTCCTCCTCCCAAACCTGGCTCAGCTCCATCCGCGCCCCCAAAAACCCTAACCCCAATCCCAACCCCAACTCCAACCCTAACGAGAACGACGATGGCGATGCCATGGTTGGACCGCCCCCTCCGCCGCCGCAATCCGAGCTTGGAGACGACGACGATGGTGAGTTGATTGGACCGCCGAGGCCGCCGGCTGGGTCCAATAGGGACGATTCCGATTCCGATTCTGATTCGGAGGCGAATTTCGAAAACAGGTTCCGAATTCCGGTGAGCAATGAGATTGTTCTCAAGGGACACACAAAAGTCGTTTCCGCACTCGCCATCGATCACACTGGGTCTCGAGTTGTTTCAGGCAGCTACGACTACTCCGTGCGGATGTACGATTTCCAGGGAATGAATTCAAAGTTGCAGTCTTTCCGGCAGCTCGAGCCATCAGAAGGCCACCAAGTTCGAACCATCAGCTGGAGCCCCACGGCGGACCGGTTTCTCTGTGTTACTGGCTCGGCTACGGCGAAGATCTATGATCGTGATGGACTTACTTTGGGAGAGTTTGTAAAAGGGGACATGTATATTCGTGATCTCAAGAACACCAAGGGCCACATAACTGGATTGACTTGTGGAGAGTGGAATCCTAAAAGTAAAGACACGATTTTAACTTCGTCCGAGGATGGATCTCTACGTATTTGGGATGTCAATGACTTCAAAAGTCAGAAACAGGTAATCAAGCCAAAACTTGCTCGGCCTGGAAGAGTTCCGGTCACCACTTGTGCTTGGGATCGCGACGGAAAACGTATTGCAGGTGGTATAGGAGATGGTTCTATACAGGTATGGAACCTTAAGCCTGGATGGGGAAGCAGGCCAGATATACATGTTCAGAATAGTCATTCAGACGATATTACCGGGCTAAAATTTTCTATGGATGGGAGAATCTTACTATCAAGAAGCCTTGATGGGACATTGAAGGTTTGGGATTTGCGTCAGATGAAGGATCCTCTTAAGGCATTTGAGGATCTCCCAAACAACTATGCCCAAACTAATGTAGCTTTTAGTCCCAATGAGCAACTCTTCTTGACTGGAACGTCTATTGAAAGGGAGAGCACCACTGGAGGTTTGCTATGCTTCTTTGATCGAGCAAAACTTGAACTTGTTTCAAAAGTTGGGATATCCCCCACCTGTAGTGTTGTGCAGTGTGCCTGGCACCCAAAACTCAATCAGATTTTTGCAACATCAGGAGATAAAAGCCAAGGAGGAACTCATGTGTTATACGATCCAACCCTTAGTGAAAGAGGAGCCCTTGTATGTGTTGCGCGTGCCCCAAGGAAAAAGTCTATGGATGATTTTGAGGCAAAACCAGTGGTTCACAACCCTCATGCTCTACCCTTATTCAGAGATCAGCCAAGCCGTAAGCGTGAGCGAGAGAAAGCACTGAAGGACCCACTGAAATCCCATAAGCCTGAACTCCCCATGACCGGACCAGGTTTTGGTGGAAGAGTTGGCGCAAGTAAAGGAAGCTTATTGACCCAGTACCTCATGAAGCAAGGGGGTATGATCAAGGAGACGTGGATGGACGAAGATCCGAGAGAAGCCATACTGAAGTATGCCGATGTTGCAGTAAAAGAACCAAAATACATTGCTCCAGCTTATGCACAAACCCAGCCTGAAACAGTTTTTGCAAAATCAGACTCTGAGGATGAAGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7 WD40

Protein Analysis

629

Amino Acids

68.99

Weight (kDa)

6.35

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 148 - 231 4.9e-07 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 152 - 238 6.7e-07 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 153 - 192 8.1e-06 WDR3 first beta-propeller domain
WD40 PF00400 156 - 189 8.8e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 156 - 236 1.7e-06 WDR5 beta-propeller domain
Beta-prop_EML PF23409 159 - 422 1.4e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 167 - 421 5.9e-21 Prp19 WD40 domain
Beta-prop_WDR35_TULP_N PF24797 206 - 388 8.3e-06 WDR35/TULP4 N-terminal
Beta-prop_THOC3 PF25174 209 - 301 2.8e-08 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 231 - 388 1.5e-12 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 256 - 421 1.3e-22 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 257 - 419 2.1e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 259 - 421 5.4e-16 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 265 - 476 3.3e-14 CDC20/Fizzy WD40 domain
Beta-prop_Aladin PF25460 265 - 389 8.5e-08 Aladin seven-bladed propeller
Beta-prop_EML_2 PF23414 274 - 389 1.9e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 274 - 390 1.9e-06 WDR90/POC16, second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 274 - 386 5.5e-07 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 312 - 412 5.8e-08 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 314 - 475 9e-16 THOC3 beta-propeller domain
WD40 PF00400 350 - 385 2.1e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20330
fragaria_vesca FvH4_2g20290 FvH4_2g20290
malus_domestica MD10G1174900.v1.1
prunus_persica Prupe.8G213600_v2.0.a1
pyrus_communis pycom05g17160 pycom10g15070
rosa_chinensis RchiOBHm_Chr6g0286091 RchiOBHm_Chr6g0286201
rosa_laevigata RLG00000012583
rosa_multiflora Rmu_sc0028702.1_g000001
rosa_roxburghii Rroxscaffold_7G00182460
rosa_rugosa Rorug06G0178900
rosa_samantha Rh6AG291300 Rh6BG294600 Rh6CG294200 Rh6CG294800 Rh6DG286200 Rh6DG286800
rosa_wichuraiana Rw6G025100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 971
AasI GACNNNNNNGTC 1 cut(s) 1261
Acc36I ACCTGC 1 cut(s) 971
AccB1I GGYRCC 1 cut(s) 1380
AccB7I CCANNNNNTGG 2 cut(s) 645, 1664
AccII CGCG 3 cut(s) 222, 963, 1492
AccIII TCCGGA 1 cut(s) 136
AclWI GGATC 7 cut(s) 862, 966, 1166, 1179, 1200, 1445, 1751
AcsI RAATTY 4 cut(s) 424, 444, 580, 1091
AcuI CTGAAG 2 cut(s) 1631, 1796
AfaI GTAC 3 cut(s) 26, 563, 1709
AfiI CCNNNNNNNGG 9 cut(s) 204, 377, 448, 620, 645, 658, 1031, 1652, 1664
AflII CTTAAG 2 cut(s) 1019, 1178
AflIII ACRYGT 2 cut(s) 747, 1051
AgeI ACCGGT 1 cut(s) 663
AgsI TTSAA 6 cut(s) 585, 886, 1144, 1271, 1325, 1335
AjiI CACGTC 1 cut(s) 1743
AjnI CCWGG 6 cut(s) 203, 570, 925, 1024, 1376, 1657
AluBI AGCT 9 cut(s) 41, 213, 328, 540, 607, 645, 1226, 1695, 1823
AluI AGCT 9 cut(s) 41, 213, 328, 540, 607, 645, 1226, 1695, 1823
Alw21I GWGCWC 1 cut(s) 1283
Alw26I GTCTC 4 cut(s) 81, 327, 525, 1733
AlwI GGATC 7 cut(s) 862, 966, 1166, 1179, 1200, 1445, 1751
AlwNI CAGNNNCTG 1 cut(s) 1868
Ama87I CYCGRG 2 cut(s) 522, 608
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 6 cut(s) 172, 368, 622, 778, 923, 1040
ApeKI GCWGC 3 cut(s) 41, 537, 604
ApoI RAATTY 4 cut(s) 424, 444, 580, 1091
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
AsiGI ACCGGT 1 cut(s) 663
Asp700I GAANNNNTTC 2 cut(s) 96, 580
AspLEI GCGC 3 cut(s) 224, 1492, 1682
AspS9I GGNCC 7 cut(s) 299, 359, 381, 661, 778, 1615, 1655
AsuC2I CCSGG 1 cut(s) 1084
AsuHPI GGTGA 3 cut(s) 359, 463, 934
AsuII TTCGAA 2 cut(s) 429, 634
AvaI CYCGRG 2 cut(s) 522, 608
AvaII GGWCC 6 cut(s) 299, 359, 381, 661, 1615, 1655
BaeGI GKGCMC 1 cut(s) 1498
BamHI GGATCC 1 cut(s) 1171
BanI GGYRCC 1 cut(s) 1380
BanII GRGCYC 2 cut(s) 653, 1477
Bbv12I GWGCWC 1 cut(s) 1283
BbvI GCAGC 3 cut(s) 28, 549, 616
BceAI ACGGC 3 cut(s) 672, 708, 1569
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BciT130I CCWGG 6 cut(s) 205, 572, 927, 1026, 1378, 1659
BclI TGATCA 1 cut(s) 1731
BcnI CCSGG 1 cut(s) 1084
BcoDI GTCTC 4 cut(s) 81, 327, 525, 1733
BfmI CTRYAG 1 cut(s) 1357
BfrI CTTAAG 2 cut(s) 1019, 1178
BfuAI ACCTGC 1 cut(s) 971
BglII AGATCT 1 cut(s) 699
BisI GCNGC 7 cut(s) 42, 156, 314, 317, 371, 538, 605
BlpI GCTNAGC 1 cut(s) 209
BlsI GCNGC 7 cut(s) 43, 157, 315, 318, 372, 539, 606
Bme1390I CCNGG 7 cut(s) 205, 572, 927, 1026, 1084, 1378, 1659
Bme18I GGWCC 6 cut(s) 299, 359, 381, 661, 1615, 1655
BmeT110I CYCGRG 2 cut(s) 522, 608
BmgBI CACGTC 1 cut(s) 1743
BmgT120I GGNCC 7 cut(s) 299, 359, 381, 661, 778, 1615, 1655
BmiI GGNNCC 8 cut(s) 382, 440, 650, 1016, 1173, 1382, 1474, 1617
BmrFI CCNGG 7 cut(s) 205, 572, 927, 1026, 1084, 1378, 1659
BmrI ACTGGG 2 cut(s) 525, 1699
BmsI GCATC 1 cut(s) 277
BmuI ACTGGG 2 cut(s) 525, 1699
BpmI CTGGAG 3 cut(s) 667, 1308, 1803
Bpu1102I GCTNAGC 1 cut(s) 209
Bpu14I TTCGAA 2 cut(s) 429, 634
BpuEI CTTGAG 2 cut(s) 455, 749
BpuMI CCSGG 1 cut(s) 1084
Bsa29I ATCGAT 1 cut(s) 507
BsaAI YACGTR 1 cut(s) 863
BsaI GGTCTC 1 cut(s) 525
BsaJI CCNNGG 8 cut(s) 291, 365, 571, 654, 774, 846, 1426, 1499
BsaWI WCCGGW 5 cut(s) 136, 448, 663, 937, 1652
BsaXI ACNNNNNCTCC 8 cut(s) 242, 272, 533, 563, 720, 750, 1464, 1494
Bsc4I CCNNNNNNNGG 9 cut(s) 204, 377, 448, 620, 645, 658, 1031, 1652, 1664
Bse118I RCCGGY 3 cut(s) 170, 373, 663
Bse1I ACTGG 7 cut(s) 520, 685, 793, 1261, 1291, 1535, 1705
Bse3DI GCAATG 2 cut(s) 463, 1812
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 6 cut(s) 205, 572, 927, 1026, 1378, 1659
BseCI ATCGAT 1 cut(s) 507
BseDI CCNNGG 8 cut(s) 291, 365, 571, 654, 774, 846, 1426, 1499
BseGI GGATG 9 cut(s) 216, 564, 856, 877, 1034, 1108, 1522, 1752, 1879
BseLI CCNNNNNNNGG 9 cut(s) 204, 377, 448, 620, 645, 658, 1031, 1652, 1664
BseMI GCAATG 2 cut(s) 463, 1812
BseMII CTCAG 2 cut(s) 223, 1860
BseNI ACTGG 7 cut(s) 520, 685, 793, 1261, 1291, 1535, 1705
BseRI GAGGAG 5 cut(s) 139, 178, 181, 184, 1485
BseSI GKGCMC 1 cut(s) 1498
BseXI GCAGC 3 cut(s) 28, 549, 616
BseYI CCCAGC 2 cut(s) 377, 1836
BsgI GTGCAG 1 cut(s) 1388
Bsh1236I CGCG 3 cut(s) 222, 963, 1492
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 6 cut(s) 174, 370, 624, 780, 925, 1042
BshNI GGYRCC 1 cut(s) 1380
BshTI ACCGGT 1 cut(s) 663
BshVI ATCGAT 1 cut(s) 507
BsiEI CGRYCG 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 1283
BsiHKCI CYCGRG 2 cut(s) 522, 608
BsiSI CCGG 9 cut(s) 137, 171, 374, 449, 601, 664, 938, 1083, 1653
BslFI GGGAC 5 cut(s) 404, 489, 758, 1150, 1218
BslI CCNNNNNNNGG 9 cut(s) 204, 377, 448, 620, 645, 658, 1031, 1652, 1664
BsmAI GTCTC 4 cut(s) 81, 327, 525, 1733
BsmBI CGTCTC 2 cut(s) 327, 1733
BsmFI GGGAC 5 cut(s) 404, 489, 758, 1150, 1218
BsnI GGCC 6 cut(s) 174, 370, 624, 780, 925, 1042
Bso31I GGTCTC 1 cut(s) 525
BsoBI CYCGRG 2 cut(s) 522, 608
Bsp119I TTCGAA 2 cut(s) 429, 634
Bsp1286I GDGCHC 4 cut(s) 653, 1283, 1477, 1498
Bsp13I TCCGGA 1 cut(s) 136
Bsp1720I GCTNAGC 1 cut(s) 209
Bsp19I CCATGG 1 cut(s) 291
Bsp68I TCGCGA 1 cut(s) 963
BspANI GGCC 6 cut(s) 174, 370, 624, 780, 925, 1042
BspCNI CTCAG 2 cut(s) 222, 1861
BspDI ATCGAT 1 cut(s) 507
BspEI TCCGGA 1 cut(s) 136
BspFNI CGCG 3 cut(s) 222, 963, 1492
BspHI TCATGA 1 cut(s) 1713
BspLI GGNNCC 8 cut(s) 382, 440, 650, 1016, 1173, 1382, 1474, 1617
BspMI ACCTGC 1 cut(s) 971
BspPI GGATC 7 cut(s) 862, 966, 1166, 1179, 1200, 1445, 1751
BspT104I TTCGAA 2 cut(s) 429, 634
BspT107I GGYRCC 1 cut(s) 1380
BspTI CTTAAG 2 cut(s) 1019, 1178
BspTNI GGTCTC 1 cut(s) 525
BsrDI GCAATG 2 cut(s) 463, 1812
BsrFI RCCGGY 3 cut(s) 170, 373, 663
BsrI ACTGG 7 cut(s) 520, 685, 793, 1261, 1291, 1535, 1705
BssAI RCCGGY 3 cut(s) 170, 373, 663
BssECI CCNNGG 8 cut(s) 291, 365, 571, 654, 774, 846, 1426, 1499
BssT1I CCWWGG 4 cut(s) 291, 774, 1426, 1499
Bst2UI CCWGG 6 cut(s) 205, 572, 927, 1026, 1378, 1659
Bst4CI ACNGT 1 cut(s) 1849
Bst6I CTCTTC 4 cut(s) 925, 1253, 1666, 1872
BstAFI CTTAAG 2 cut(s) 1019, 1178
BstBAI YACGTR 1 cut(s) 863
BstBI TTCGAA 2 cut(s) 429, 634
BstC8I GCNNGC 3 cut(s) 375, 1040, 1494
BstDEI CTNAG 4 cut(s) 72, 209, 1460, 1869
BstDSI CCRYGG 2 cut(s) 291, 654
BstEII GGTNACC 1 cut(s) 940
BstF5I GGATG 9 cut(s) 216, 564, 856, 877, 1034, 1108, 1522, 1752, 1879
BstFNI CGCG 3 cut(s) 222, 963, 1492
BstHHI GCGC 3 cut(s) 224, 1492, 1682
BstMAI GTCTC 4 cut(s) 81, 327, 525, 1733
BstMCI CGRYCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 6 cut(s) 219, 310, 325, 370, 610, 1790
BstNI CCWGG 6 cut(s) 205, 572, 927, 1026, 1378, 1659
BstNSI RCATGY 2 cut(s) 751, 1055
BstPI GGTNACC 1 cut(s) 940
BstSCI CCNGG 7 cut(s) 203, 570, 925, 1024, 1082, 1376, 1657
BstSFI CTRYAG 1 cut(s) 1357
BstSLI GKGCMC 1 cut(s) 1498
BstSNI TACGTA 1 cut(s) 863
BstUI CGCG 3 cut(s) 222, 963, 1492
BstV1I GCAGC 3 cut(s) 28, 549, 616
BstX2I RGATCY 5 cut(s) 699, 854, 1171, 1192, 1756
BstXI CCANNNNNNTGG 1 cut(s) 788
BstYI RGATCY 5 cut(s) 699, 854, 1171, 1192, 1756
Bsu15I ATCGAT 1 cut(s) 507
BsuRI GGCC 6 cut(s) 174, 370, 624, 780, 925, 1042
BsuTUI ATCGAT 1 cut(s) 507
BtgI CCRYGG 2 cut(s) 291, 654
BtgZI GCGATG 1 cut(s) 300
BtrI CACGTC 1 cut(s) 1743
BtsCI GGATG 9 cut(s) 216, 564, 856, 877, 1034, 1108, 1522, 1752, 1879
BtsI GCAGTG 1 cut(s) 1376
BtsIMutI CAGTG 6 cut(s) 513, 1284, 1376, 1542, 1607, 1619
BtuMI TCGCGA 1 cut(s) 963
BveI ACCTGC 1 cut(s) 971
Cac8I GCNNGC 3 cut(s) 375, 1040, 1494
CaiI CAGNNNCTG 1 cut(s) 1868
CciI TCATGA 1 cut(s) 1713
CfoI GCGC 3 cut(s) 224, 1492, 1682
Cfr10I RCCGGY 3 cut(s) 170, 373, 663
Cfr13I GGNCC 7 cut(s) 299, 359, 381, 661, 778, 1615, 1655
ClaI ATCGAT 1 cut(s) 507
CpoI CGGWCCG 1 cut(s) 661
CseI GACGC 1 cut(s) 1148
CsiI ACCWGGT 1 cut(s) 1657
Csp6I GTAC 3 cut(s) 25, 562, 1708
CspAI ACCGGT 1 cut(s) 663
CspCI CAANNNNNGTGG 2 cut(s) 1518, 1553
CspI CGGWCCG 1 cut(s) 661
CviAII CATG 7 cut(s) 292, 748, 1052, 1439, 1553, 1648, 1714
CviQI GTAC 3 cut(s) 25, 562, 1708
DdeI CTNAG 4 cut(s) 72, 209, 1460, 1869
DrdI GACNNNNNNGTC 1 cut(s) 1261
DseDI GACNNNNNNGTC 1 cut(s) 1261
Eam1104I CTCTTC 4 cut(s) 925, 1253, 1666, 1872
EarI CTCTTC 4 cut(s) 925, 1253, 1666, 1872
EciI GGCGGA 4 cut(s) 20, 142, 300, 674
Eco105I TACGTA 1 cut(s) 863
Eco130I CCWWGG 4 cut(s) 291, 774, 1426, 1499
Eco24I GRGCYC 2 cut(s) 653, 1477
Eco31I GGTCTC 1 cut(s) 525
Eco32I GATATC 1 cut(s) 1347
Eco47I GGWCC 6 cut(s) 299, 359, 381, 661, 1615, 1655
Eco57I CTGAAG 2 cut(s) 1631, 1796
Eco88I CYCGRG 2 cut(s) 522, 608
Eco91I GGTNACC 1 cut(s) 940
EcoO109I RGGNCCY 1 cut(s) 1615
EcoO65I GGTNACC 1 cut(s) 940
EcoRI GAATTC 2 cut(s) 444, 580
EcoRII CCWGG 6 cut(s) 203, 570, 925, 1024, 1376, 1657
EcoRV GATATC 1 cut(s) 1347
EcoT14I CCWWGG 4 cut(s) 291, 774, 1426, 1499
EcoT38I GRGCYC 2 cut(s) 653, 1477
ErhI CCWWGG 4 cut(s) 291, 774, 1426, 1499
Esp3I CGTCTC 2 cut(s) 327, 1733
FaeI CATG 7 cut(s) 295, 751, 1055, 1442, 1556, 1651, 1717
FaqI GGGAC 5 cut(s) 404, 489, 758, 1150, 1218
FatI CATG 7 cut(s) 291, 747, 1051, 1438, 1552, 1647, 1713
FbaI TGATCA 1 cut(s) 1731
Fnu4HI GCNGC 7 cut(s) 42, 156, 314, 317, 371, 538, 605
FokI GGATG 8 cut(s) 203, 571, 863, 884, 1041, 1115, 1529, 1759
FriOI GRGCYC 2 cut(s) 653, 1477
Fsp4HI GCNGC 7 cut(s) 42, 156, 314, 317, 371, 538, 605
GlaI GCGC 3 cut(s) 223, 1491, 1681
GluI GCNGC 7 cut(s) 42, 156, 314, 317, 371, 538, 605
GsaI CCCAGC 2 cut(s) 381, 1840
GsuI CTGGAG 3 cut(s) 667, 1308, 1803
HaeIII GGCC 6 cut(s) 174, 370, 624, 780, 925, 1042
HapII CCGG 9 cut(s) 137, 171, 374, 449, 601, 664, 938, 1083, 1653
HgaI GACGC 1 cut(s) 1148
HhaI GCGC 3 cut(s) 224, 1492, 1682
Hin1II CATG 7 cut(s) 295, 751, 1055, 1442, 1556, 1651, 1717
Hin6I GCGC 3 cut(s) 222, 1490, 1680
HinP1I GCGC 3 cut(s) 222, 1490, 1680
HindIII AAGCTT 1 cut(s) 1693
HinfI GANTC 9 cut(s) 33, 109, 395, 401, 407, 413, 811, 1110, 1865
HpaII CCGG 9 cut(s) 137, 171, 374, 449, 601, 664, 938, 1083, 1653
HphI GGTGA 3 cut(s) 359, 463, 934
Hpy166II GTNNAC 1 cut(s) 1543
Hpy8I GTNNAC 1 cut(s) 1543
Hpy99I CGWCG 7 cut(s) 14, 32, 117, 281, 341, 344, 968
HpyAV CCTTC 5 cut(s) 189, 614, 1138, 1162, 1606
HpyCH4III ACNGT 1 cut(s) 1849
HpyCH4IV ACGT 4 cut(s) 862, 973, 1262, 1742
HpyCH4V TGCA 7 cut(s) 592, 980, 1369, 1406, 1793, 1829, 1856
HpyF10VI GCNNNNNNNGC 6 cut(s) 219, 310, 325, 370, 610, 1790
HpyF3I CTNAG 4 cut(s) 72, 209, 1460, 1869
HpySE526I ACGT 4 cut(s) 862, 973, 1262, 1742
Hsp92II CATG 7 cut(s) 295, 751, 1055, 1442, 1556, 1651, 1717
HspAI GCGC 3 cut(s) 222, 1490, 1680
Kpn2I TCCGGA 1 cut(s) 136
KroI GCCGGC 1 cut(s) 373
KroNI GCCGGC 1 cut(s) 375
Ksp22I TGATCA 1 cut(s) 1731
LmnI GCTCC 4 cut(s) 218, 648, 1472, 1822
Lsp1109I GCAGC 3 cut(s) 28, 549, 616
LweI GCATC 1 cut(s) 277
MabI ACCWGGT 1 cut(s) 1657
MaeII ACGT 4 cut(s) 862, 973, 1262, 1742
MaeIII GTNAC 2 cut(s) 676, 940
MboII GAAGA 5 cut(s) 709, 942, 1240, 1683, 1766
MflI RGATCY 5 cut(s) 699, 854, 1171, 1192, 1756
MhlI GDGCHC 4 cut(s) 653, 1283, 1477, 1498
MluCI AATT 4 cut(s) 424, 444, 580, 1091
MlyI GAGTC 2 cut(s) 103, 1859
MmeI TCCRAC 3 cut(s) 277, 285, 1478
MroI TCCGGA 1 cut(s) 136
MroNI GCCGGC 1 cut(s) 373
MroXI GAANNNNTTC 2 cut(s) 96, 580
MseI TTAA 3 cut(s) 836, 1020, 1179
MspA1I CMGCKG 1 cut(s) 645
MspCI CTTAAG 2 cut(s) 1019, 1178
MspI CCGG 9 cut(s) 137, 171, 374, 449, 601, 664, 938, 1083, 1653
MspR9I CCNGG 7 cut(s) 205, 572, 927, 1026, 1084, 1378, 1659
MvaI CCWGG 6 cut(s) 205, 572, 927, 1026, 1378, 1659
MvnI CGCG 3 cut(s) 222, 963, 1492
MwoI GCNNNNNNNGC 6 cut(s) 219, 310, 325, 370, 610, 1790
NaeI GCCGGC 1 cut(s) 375
NciI CCSGG 1 cut(s) 1084
NcoI CCATGG 1 cut(s) 291
NgoMIV GCCGGC 1 cut(s) 373
NlaIII CATG 7 cut(s) 295, 751, 1055, 1442, 1556, 1651, 1717
NlaIV GGNNCC 8 cut(s) 382, 440, 650, 1016, 1173, 1382, 1474, 1617
NmeAIII GCCGAG 3 cut(s) 390, 665, 901
NmuCI GTSAC 1 cut(s) 940
NruI TCGCGA 1 cut(s) 963
NspI RCATGY 2 cut(s) 751, 1055
NspV TTCGAA 2 cut(s) 429, 634
PaeR7I CTCGAG 2 cut(s) 522, 608
PagI TCATGA 1 cut(s) 1713
PaqCI CACCTGC 1 cut(s) 971
PciI ACATGT 2 cut(s) 747, 1051
PcsI WCGNNNNNNNCGW 4 cut(s) 276, 282, 549, 692
PdiI GCCGGC 1 cut(s) 375
PdmI GAANNNNTTC 2 cut(s) 96, 580
PfeI GAWTC 7 cut(s) 33, 395, 401, 407, 413, 811, 1110
PflMI CCANNNNNTGG 2 cut(s) 645, 1664
PinAI ACCGGT 1 cut(s) 663
PkrI GCNGC 7 cut(s) 43, 157, 315, 318, 372, 539, 606
PleI GAGTC 2 cut(s) 103, 1859
PpsI GAGTC 2 cut(s) 103, 1859
Ppu21I YACGTR 1 cut(s) 863
PpuMI RGGWCCY 1 cut(s) 1615
PscI ACATGT 2 cut(s) 747, 1051
Psp5II RGGWCCY 1 cut(s) 1615
Psp6I CCWGG 6 cut(s) 203, 570, 925, 1024, 1376, 1657
PspEI GGTNACC 1 cut(s) 940
PspFI CCCAGC 2 cut(s) 377, 1836
PspGI CCWGG 6 cut(s) 203, 570, 925, 1024, 1376, 1657
PspN4I GGNNCC 8 cut(s) 382, 440, 650, 1016, 1173, 1382, 1474, 1617
PspPI GGNCC 7 cut(s) 299, 359, 381, 661, 778, 1615, 1655
PspPPI RGGWCCY 1 cut(s) 1615
PspXI VCTCGAGB 1 cut(s) 608
PstNI CAGNNNCTG 1 cut(s) 1868
PsuI RGATCY 5 cut(s) 699, 854, 1171, 1192, 1756
PvuII CAGCTG 1 cut(s) 645
RruI TCGCGA 1 cut(s) 963
RsaI GTAC 3 cut(s) 26, 563, 1709
RsaNI GTAC 3 cut(s) 25, 562, 1708
Rsr2I CGGWCCG 1 cut(s) 661
RsrII CGGWCCG 1 cut(s) 661
SaqAI TTAA 3 cut(s) 836, 1020, 1179
SatI GCNGC 7 cut(s) 42, 156, 314, 317, 371, 538, 605
Sau96I GGNCC 7 cut(s) 299, 359, 381, 661, 778, 1615, 1655
SchI GAGTC 2 cut(s) 103, 1859
ScrFI CCNGG 7 cut(s) 205, 572, 927, 1026, 1084, 1378, 1659
SduI GDGCHC 4 cut(s) 653, 1283, 1477, 1498
SexAI ACCWGGT 1 cut(s) 1657
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 1 cut(s) 1357
Sfr274I CTCGAG 2 cut(s) 522, 608
SfuI TTCGAA 2 cut(s) 429, 634
SinI GGWCC 6 cut(s) 299, 359, 381, 661, 1615, 1655
SlaI CTCGAG 2 cut(s) 522, 608
SmlI CTYRAG 6 cut(s) 470, 522, 608, 764, 1019, 1178
SmoI CTYRAG 6 cut(s) 470, 522, 608, 764, 1019, 1178
SnaBI TACGTA 1 cut(s) 863
Sse9I AATT 4 cut(s) 424, 444, 580, 1091
StyD4I CCNGG 7 cut(s) 203, 570, 925, 1024, 1082, 1376, 1657
StyI CCWWGG 4 cut(s) 291, 774, 1426, 1499
TaaI ACNGT 1 cut(s) 1849
TaiI ACGT 4 cut(s) 865, 976, 1265, 1745
TaqI TCGA 7 cut(s) 90, 429, 507, 523, 609, 634, 1312
TasI AATT 4 cut(s) 424, 444, 580, 1091
TauI GCSGC 4 cut(s) 158, 316, 319, 373
TfiI GAWTC 7 cut(s) 33, 395, 401, 407, 413, 811, 1110
Tru1I TTAA 3 cut(s) 836, 1020, 1179
Tru9I TTAA 3 cut(s) 836, 1020, 1179
TscAI CASTG 6 cut(s) 520, 1291, 1376, 1542, 1614, 1626
TseFI GTSAC 1 cut(s) 940
TseI GCWGC 3 cut(s) 41, 537, 604
Tsp45I GTSAC 1 cut(s) 940
TspDTI ATGAA 3 cut(s) 593, 1181, 1730
TspGWI ACGGA 4 cut(s) 45, 109, 541, 981
TspRI CASTG 6 cut(s) 520, 1291, 1376, 1542, 1614, 1626
Van91I CCANNNNNTGG 2 cut(s) 645, 1664
Vha464I CTTAAG 2 cut(s) 1019, 1178
VpaK11BI GGWCC 6 cut(s) 299, 359, 381, 661, 1615, 1655
XapI RAATTY 4 cut(s) 424, 444, 580, 1091
XceI RCATGY 2 cut(s) 751, 1055
XhoI CTCGAG 2 cut(s) 522, 608
XmnI GAANNNNTTC 2 cut(s) 96, 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.