Rh6CG294800

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0011288
Physical Location & Seq
Reverse (-)
47345022 .. 47355779
10758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG294800.1

Sequence Viewer

Length: 843 bp
ATGGTATCATGTTCCTCGACCAAGAACCGAGGCAAACTGAAGGAGCAAAAGAAGCCTAAACTTCGCCTAGAGTGTACAAACGGGCAATCCATTTCTACTATTAAATTTTCAAGCTTCGGTACTCCACCTGGAAGCTATGGGAACTGCAACCATGGTACCTGTCACGCTCCAAACTCTACCGATCTGCTGGATAAGCCTGATTCGGCCTCCGACGACGACAGCGATGCCCTCATCGGACCGCCGCCGCCTCCCGGGAGTGCCGAGAGCGACGACGATGGTGAGATGATTGGACCGCCGGCGCCGGGGAGGTCCAATAGAGACGATTGGGATTCCGATGCGGAAGAGGAGAACAAGTACCGGATTCCGGTGAGCAATGAGATTGTGCTGAAGGGACACACCAAGGTGGTTTCGGCCCTCGCCATTGATCACACTGGTTCTAGAGTTATATCTGGTAGCTATGACCATTCTGTGAGAATGTACGATTTTCAAGGAATGAATGCAAAGTTGCAATCTTTCCGGCAGCTGGAGCCGTCTGAAGGTCATCAAAAGGACTCTAATGTCTCCGGCGACGATGCCTTCGCTCGCCTCTACTCTGCCGTCGAGTTCGACATTGAAGCTGCTCTTCAGAAAGCGGAGCTTGCTTCAAAAGAGAAAAGTAGGGCATTTGCAGTTGCTCTGAATGTCGTGATTCGCCGGACCAAGGCTCGATTGCTTAAAGAGGTTTCCAAGTTGCAGAGATCGGCTGTAAAGAAGAGTACAAAATATTTGGTGAAAGGCAATGATACCCTAGATGGGACGCCAGTTGAGTACAACAAGATGTTGAAGCCAAGCCATGAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7 WD40

Protein Analysis

280

Amino Acids

30.57

Weight (kDa)

6.67

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 125 - 163 2.8e-06 WDR3 first beta-propeller domain
WD40 PF00400 128 - 161 7.7e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20330
fragaria_vesca FvH4_2g20290 FvH4_2g20290
malus_domestica MD10G1174900.v1.1
prunus_persica Prupe.8G213600_v2.0.a1
pyrus_communis pycom05g17160 pycom10g15070
rosa_chinensis RchiOBHm_Chr6g0286091 RchiOBHm_Chr6g0286201
rosa_laevigata RLG00000012583
rosa_multiflora Rmu_sc0028702.1_g000001
rosa_roxburghii Rroxscaffold_7G00182460
rosa_rugosa Rorug06G0178900
rosa_samantha Rh6AG291300 Rh6BG294600 Rh6CG294200 Rh6CG294800 Rh6DG286200 Rh6DG286800
rosa_wichuraiana Rw6G025100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 557
Acc65I GGTACC 1 cut(s) 155
AccB1I GGYRCC 2 cut(s) 155, 298
AciI CCGC 6 cut(s) 239, 242, 245, 293, 338, 632
AcsI RAATTY 1 cut(s) 104
AcuI CTGAAG 4 cut(s) 59, 407, 555, 608
AcyI GRCGYC 2 cut(s) 299, 797
AfaI GTAC 7 cut(s) 76, 121, 157, 356, 479, 757, 809
AfiI CCNNNNNNNGG 7 cut(s) 251, 302, 364, 523, 536, 700, 792
AgsI TTSAA 5 cut(s) 111, 488, 614, 645, 823
AjnI CCWGG 1 cut(s) 127
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 6 cut(s) 114, 135, 456, 523, 617, 637
AluI AGCT 6 cut(s) 114, 135, 456, 523, 617, 637
Alw26I GTCTC 2 cut(s) 312, 565
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 2 cut(s) 204, 411
ApeKI GCWGC 2 cut(s) 520, 617
ApoI RAATTY 1 cut(s) 104
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
Asp718I GGTACC 1 cut(s) 155
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 5 cut(s) 236, 290, 309, 412, 696
AsuC2I CCSGG 3 cut(s) 252, 253, 303
AsuHPI GGTGA 3 cut(s) 290, 379, 781
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 4 cut(s) 236, 290, 309, 696
BaeI ACNNNNGTAYC 2 cut(s) 139, 172
BanI GGYRCC 2 cut(s) 155, 298
BbvI GCAGC 2 cut(s) 532, 604
BccI CCATC 2 cut(s) 269, 785
BceAI ACGGC 2 cut(s) 514, 581
BcgI CGANNNNNNTGC 2 cut(s) 206, 240
BciT130I CCWGG 1 cut(s) 129
BclI TGATCA 1 cut(s) 424
BcnI CCSGG 3 cut(s) 252, 253, 303
BcoDI GTCTC 2 cut(s) 312, 565
BfaI CTAG 3 cut(s) 68, 438, 788
BfoI RGCGCY 1 cut(s) 302
BisI GCNGC 4 cut(s) 242, 245, 521, 618
BlsI GCNGC 4 cut(s) 243, 246, 522, 619
Bme1390I CCNGG 4 cut(s) 129, 252, 253, 303
Bme18I GGWCC 4 cut(s) 236, 290, 309, 696
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 5 cut(s) 236, 290, 309, 412, 696
BmiI GGNNCC 3 cut(s) 157, 300, 528
BmrFI CCNGG 4 cut(s) 129, 252, 253, 303
BmsI GCATC 3 cut(s) 214, 325, 562
BpmI CTGGAG 1 cut(s) 545
BpuMI CCSGG 3 cut(s) 252, 253, 303
BsaHI GRCGYC 2 cut(s) 299, 797
BsaJI CCNNGG 6 cut(s) 28, 151, 251, 302, 399, 699
BsaWI WCCGGW 2 cut(s) 357, 364
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 7 cut(s) 251, 302, 364, 523, 536, 700, 792
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 2 cut(s) 436, 800
Bse3DI GCAATG 2 cut(s) 379, 784
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 6 cut(s) 28, 151, 251, 302, 399, 699
BseLI CCNNNNNNNGG 7 cut(s) 251, 302, 364, 523, 536, 700, 792
BseMI GCAATG 2 cut(s) 379, 784
BseNI ACTGG 2 cut(s) 436, 800
BseRI GAGGAG 1 cut(s) 359
BseXI GCAGC 2 cut(s) 532, 604
BshFI GGCC 2 cut(s) 206, 413
BshNI GGYRCC 2 cut(s) 155, 298
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 8 cut(s) 252, 296, 302, 358, 365, 517, 564, 694
BslFI GGGAC 2 cut(s) 405, 808
BslI CCNNNNNNNGG 7 cut(s) 251, 302, 364, 523, 536, 700, 792
BsmAI GTCTC 2 cut(s) 312, 565
BsmBI CGTCTC 1 cut(s) 312
BsmFI GGGAC 2 cut(s) 405, 808
BsmI GAATGC 1 cut(s) 502
BsnI GGCC 2 cut(s) 206, 413
BsoBI CYCGRG 1 cut(s) 251
Bsp1407I TGTACA 1 cut(s) 74
Bsp143I GATC 3 cut(s) 181, 424, 737
Bsp19I CCATGG 1 cut(s) 151
BspACI CCGC 6 cut(s) 239, 242, 245, 293, 338, 632
BspANI GGCC 2 cut(s) 206, 413
BspLI GGNNCC 3 cut(s) 157, 300, 528
BspQI GCTCTTC 1 cut(s) 627
BspT107I GGYRCC 2 cut(s) 155, 298
BsrDI GCAATG 2 cut(s) 379, 784
BsrFI RCCGGY 1 cut(s) 295
BsrGI TGTACA 1 cut(s) 74
BsrI ACTGG 2 cut(s) 436, 800
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 6 cut(s) 28, 151, 251, 302, 399, 699
BssMI GATC 3 cut(s) 181, 424, 737
BssNI GRCGYC 2 cut(s) 299, 797
BssT1I CCWWGG 3 cut(s) 151, 399, 699
Bst2UI CCWGG 1 cut(s) 129
Bst6I CTCTTC 3 cut(s) 336, 627, 746
BstACI GRCGYC 2 cut(s) 299, 797
BstAUI TGTACA 1 cut(s) 74
BstC8I GCNNGC 3 cut(s) 297, 583, 639
BstDSI CCRYGG 1 cut(s) 151
BstH2I RGCGCY 1 cut(s) 302
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 3 cut(s) 184, 427, 740
BstMAI GTCTC 2 cut(s) 312, 565
BstMBI GATC 3 cut(s) 181, 424, 737
BstMWI GCNNNNNNNGC 4 cut(s) 52, 193, 526, 638
BstNI CCWGG 1 cut(s) 129
BstSCI CCNGG 4 cut(s) 127, 250, 251, 301
BstV1I GCAGC 2 cut(s) 532, 604
BsuRI GGCC 2 cut(s) 206, 413
BtgI CCRYGG 1 cut(s) 151
BtgZI GCGATG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 429
Cac8I GCNNGC 3 cut(s) 297, 583, 639
CfoI GCGC 1 cut(s) 301
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 5 cut(s) 236, 290, 309, 412, 696
Cfr9I CCCGGG 1 cut(s) 251
CpoI CGGWCCG 1 cut(s) 236
CseI GACGC 1 cut(s) 805
Csp6I GTAC 7 cut(s) 75, 120, 156, 355, 478, 756, 808
CspI CGGWCCG 1 cut(s) 236
CviAII CATG 3 cut(s) 9, 152, 833
CviQI GTAC 7 cut(s) 75, 120, 156, 355, 478, 756, 808
DinI GGCGCC 1 cut(s) 300
DpnI GATC 3 cut(s) 183, 426, 739
DpnII GATC 3 cut(s) 181, 424, 737
DrdI GACNNNNNNGTC 1 cut(s) 557
DseDI GACNNNNNNGTC 1 cut(s) 557
Eam1104I CTCTTC 3 cut(s) 336, 627, 746
EarI CTCTTC 3 cut(s) 336, 627, 746
Eco130I CCWWGG 3 cut(s) 151, 399, 699
Eco47I GGWCC 4 cut(s) 236, 290, 309, 696
Eco57I CTGAAG 4 cut(s) 59, 407, 555, 608
Eco88I CYCGRG 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 3 cut(s) 151, 399, 699
EgeI GGCGCC 1 cut(s) 300
EheI GGCGCC 1 cut(s) 300
ErhI CCWWGG 3 cut(s) 151, 399, 699
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 3 cut(s) 12, 155, 836
FaiI YATR 6 cut(s) 10, 138, 153, 446, 459, 834
FalI AAGNNNNNCTT 4 cut(s) 606, 638, 621, 653
FaqI GGGAC 2 cut(s) 405, 808
FatI CATG 3 cut(s) 8, 151, 832
FbaI TGATCA 1 cut(s) 424
Fnu4HI GCNGC 4 cut(s) 242, 245, 521, 618
Fsp4HI GCNGC 4 cut(s) 242, 245, 521, 618
FspBI CTAG 3 cut(s) 68, 438, 788
GlaI GCGC 1 cut(s) 300
GluI GCNGC 4 cut(s) 242, 245, 521, 618
GsuI CTGGAG 1 cut(s) 545
HaeII RGCGCY 1 cut(s) 302
HaeIII GGCC 2 cut(s) 206, 413
HapII CCGG 8 cut(s) 252, 296, 302, 358, 365, 517, 564, 694
HgaI GACGC 1 cut(s) 805
HhaI GCGC 1 cut(s) 301
Hin1I GRCGYC 2 cut(s) 299, 797
Hin1II CATG 3 cut(s) 12, 155, 836
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 5 cut(s) 200, 329, 361, 551, 688
HpaII CCGG 8 cut(s) 252, 296, 302, 358, 365, 517, 564, 694
HphI GGTGA 3 cut(s) 290, 379, 781
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 6 cut(s) 211, 236, 334, 535, 627, 678
Hpy188III TCNNGA 2 cut(s) 438, 685
Hpy8I GTNNAC 1 cut(s) 75
Hpy99I CGWCG 6 cut(s) 215, 218, 272, 275, 572, 602
HpyAV CCTTC 4 cut(s) 34, 382, 530, 586
HpyCH4V TGCA 5 cut(s) 147, 500, 508, 668, 733
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 193, 526, 638
Hsp92I GRCGYC 2 cut(s) 299, 797
Hsp92II CATG 3 cut(s) 12, 155, 836
HspAI GCGC 1 cut(s) 299
KasI GGCGCC 1 cut(s) 298
KpnI GGTACC 1 cut(s) 159
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 3 cut(s) 181, 424, 737
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 4 cut(s) 43, 172, 526, 634
Lsp1109I GCAGC 2 cut(s) 532, 604
LweI GCATC 3 cut(s) 214, 325, 562
MaeI CTAG 3 cut(s) 68, 438, 788
MaeIII GTNAC 1 cut(s) 161
MalI GATC 3 cut(s) 183, 426, 739
MboI GATC 3 cut(s) 181, 424, 737
MboII GAAGA 3 cut(s) 353, 614, 763
MluCI AATT 1 cut(s) 104
Mly113I GGCGCC 1 cut(s) 299
MlyI GAGTC 1 cut(s) 545
MmeI TCCRAC 1 cut(s) 234
MreI CGCCGGCG 1 cut(s) 295
MroNI GCCGGC 1 cut(s) 295
MseI TTAA 2 cut(s) 102, 714
MslI CAYNNNNRTG 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 523
MspI CCGG 8 cut(s) 252, 296, 302, 358, 365, 517, 564, 694
MspR9I CCNGG 4 cut(s) 129, 252, 253, 303
Mva1269I GAATGC 1 cut(s) 502
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 4 cut(s) 52, 193, 526, 638
NaeI GCCGGC 1 cut(s) 297
NarI GGCGCC 1 cut(s) 299
NciI CCSGG 3 cut(s) 252, 253, 303
NcoI CCATGG 1 cut(s) 151
NdeII GATC 3 cut(s) 181, 424, 737
NgoMIV GCCGGC 1 cut(s) 295
NlaIII CATG 3 cut(s) 12, 155, 836
NlaIV GGNNCC 3 cut(s) 157, 300, 528
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 1 cut(s) 161
OliI CACNNNNGTG 1 cut(s) 401
PciSI GCTCTTC 1 cut(s) 627
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 502
PdiI GCCGGC 1 cut(s) 297
PfeI GAWTC 4 cut(s) 200, 329, 361, 688
PkrI GCNGC 4 cut(s) 243, 246, 522, 619
PleI GAGTC 1 cut(s) 545
PluTI GGCGCC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 545
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 3 cut(s) 157, 300, 528
PspPI GGNCC 5 cut(s) 236, 290, 309, 412, 696
PvuII CAGCTG 1 cut(s) 523
RsaI GTAC 7 cut(s) 76, 121, 157, 356, 479, 757, 809
RsaNI GTAC 7 cut(s) 75, 120, 156, 355, 478, 756, 808
RseI CAYNNNNRTG 1 cut(s) 401
Rsr2I CGGWCCG 1 cut(s) 236
RsrII CGGWCCG 1 cut(s) 236
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 2 cut(s) 102, 714
SatI GCNGC 4 cut(s) 242, 245, 521, 618
Sau3AI GATC 3 cut(s) 181, 424, 737
Sau96I GGNCC 5 cut(s) 236, 290, 309, 412, 696
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 4 cut(s) 129, 252, 253, 303
SfaNI GCATC 3 cut(s) 214, 325, 562
SfoI GGCGCC 1 cut(s) 300
SgrAI CRCCGGYG 1 cut(s) 295
SinI GGWCC 4 cut(s) 236, 290, 309, 696
SmaI CCCGGG 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 401
Sse9I AATT 1 cut(s) 104
SsiI CCGC 6 cut(s) 239, 242, 245, 293, 338, 632
SspDI GGCGCC 1 cut(s) 298
SspI AATATT 1 cut(s) 764
SspMI CTAG 3 cut(s) 68, 438, 788
StyD4I CCNGG 4 cut(s) 127, 250, 251, 301
StyI CCWWGG 3 cut(s) 151, 399, 699
TaqI TCGA 4 cut(s) 17, 600, 606, 706
TasI AATT 1 cut(s) 104
TatI WGTACW 3 cut(s) 74, 755, 807
TauI GCSGC 2 cut(s) 244, 247
TfiI GAWTC 4 cut(s) 200, 329, 361, 688
Tru1I TTAA 2 cut(s) 102, 714
Tru9I TTAA 2 cut(s) 102, 714
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 2 cut(s) 520, 617
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 1 cut(s) 509
TspMI CCCGGG 1 cut(s) 251
TspRI CASTG 1 cut(s) 436
VpaK11BI GGWCC 4 cut(s) 236, 290, 309, 696
XapI RAATTY 1 cut(s) 104
XbaI TCTAGA 1 cut(s) 437
XmaI CCCGGG 1 cut(s) 251
XspI CTAG 3 cut(s) 68, 438, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.