Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004460

Physical Location & Seq

Genomic Coordinates Reverse (-)

40991064 .. 40994028

Gene Structure 2965 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1332600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1230 bp

ATGAAGACTCCAAAATCCACCCTAGATACGACGCAGCTTCAAATCCAAGCAACCGTGGCCATGGCCGAACTCCCGCCGGAGGTAATCGTCGACATACTCTCTCGTCTGCCCGTCGCCCGCCTCCTCCGCTTCCGGTCGATTGCAAAACCATGGCGGTCGCTCATCGACAGCTCATACTTCGTCCATCTCCACCTCACGCAATCGGCAGAGTCCAAATCGCATCTCAGTTTGATTCTCAAAAGGGACTCCGACCTCTACTGCATCAGTTTCGACTCCTTAAACGACGCCGTCGAGCTCAATCATCCACTCATGTGCTACAGCAATCGAATTCGCGTTTTGGGATCCTGCAACGGCCTTCTGTGCCTCTGTAACGTGGCCGAAGACATCGCTCTCTGGAACCCGTCGACGAAGAAGTACAGAATCTTGCCGTCTCTGCCATCGGATCGAAAGCGCGATTCGAACATGTGCTTATGTGGCGCGAGGGTTTATGGTCTTGGATACCACGCTATCCAGGATGACTATAAATTGGTGAGGATTTCTCAGTTTATCGGATTGGATCACCTGTCGTTTCATTCCGAGGTGAGGGTTTATAGCTTGAGAAACAACGAGTGGAAGCGAGTCGAAGACATGCCTTATGTACTCTGTAACACACGAAAAATGGGGATTCTTGTGAGCGGTTGTGTGCATTGGGTGGTGACTCGGAACCTCGAATTGGATCAAGCTGCAACCGAATCGGTCATTGCATTTGACGTTGCGAATGAGACTTTTCGTGAGGTGCCTATGCCGCAGAACATGGACAGGAAGTGTCAGATTGATGTGGGATGCTTGGGAGGGTGTCTTTGCATTGTAGCTAAATATGAAGATAAGGATGTTGATGTGTGGACATTGAAGGAGTATGGGGTTCAAGAGTCTTGGAGTAAATTGTTTACCGTTGCGCAAACTCGAAGAATCAAGAGCGTTAGGCCTTTGGTGTATTCAAAGAATGACCGCGAAGTGTTGTTCGAACAAGACCACGAAAGTCTTGTTTGGTTTGACCTGAAAAGCCAGAAGGTCAAGCGTGTTAAGGTTCGCGGCTTGCCAGCTATGTTTGAGGCTGTAGTTTGTATGGAAAGCCTTGTTCCGGTTCATCCTCGTAGACGAGAGGATGAGAAGCAGCAGGAAGCAGCAGGGGACAAGCAGAATAAGAAGAGGGATGATTTCCTGGCTCAAGGCTTCAAATTGGTGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box FBA_1

Protein Analysis

410

Amino Acids

46.94

Weight (kDa)

8.55

Isoelectric Point (pI)

45.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box-like	PF12937	22 - 58	5.1e-06	F-box-like
F-box	PF00646	23 - 61	4.1e-07	F-box domain
FBA_1	PF07734	78 - 353	1.1e-27	F-box associated beta propeller domain
FBA_3	PF08268	78 - 361	4.7e-24	F-box associated beta propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G12560 AT4G12560 AT4G12560 AT4G22390 AT4G22390
fragaria_vesca	FvH4_3g00900 FvH4_3g00900
malus_domestica	MD05G1358500.v1.1 MD10G1332600.v1.1
prunus_persica	Prupe.4G009600_v2.0.a1 Prupe.4G009600_v2.0.a1 Prupe.4G009600_v2.0.a1 Prupe.4G009600_v2.0.a1 Prupe.4G009600_v2.0.a1 Prupe.4G009600_v2.0.a1 Prupe.4G009600_v2.0.a1 Prupe.4G009600_v2.0.a1 Prupe.4G009600_v2.0.a1
pyrus_communis	pycom05g32420 pycom10g28200
rosa_chinensis	RchiOBHm_Chr5g0001381
rosa_laevigata	RLG00000030926
rosa_multiflora	Rmu_sc0001706.1_g000001 Rmu_sc0003749.1_g000016 Rmu_sc0016478.1_g000001
rosa_roxburghii	Rroxscaffold_1G00074980
rosa_rugosa	Rorug04G0390300
rosa_samantha	Rh5AG010700 Rh5BG013400 Rh5CG011700 Rh5DG011100
rosa_wichuraiana	Rw5G001020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	936
AccB1I	GGYRCC	1 cut(s)	775
AccBSI	CCGCTC	1 cut(s)	675
AccI	GTMKAC	3 cut(s)	90, 404, 1135
AccII	CGCG	5 cut(s)	333, 453, 479, 990, 1071
AciI	CCGC	8 cut(s)	74, 118, 127, 154, 675, 785, 988, 1071
AclWI	GGATC	5 cut(s)	336, 349, 450, 564, 723
AcoI	YGGCCR	3 cut(s)	57, 63, 375
AcsI	RAATTY	1 cut(s)	327
AcyI	GRCGYC	1 cut(s)	285
AfaI	GTAC	2 cut(s)	416, 639
AfiI	CCNNNNNNNGG	4 cut(s)	79, 582, 712, 1120
AflIII	ACRYGT	1 cut(s)	462
AgsI	TTSAA	5 cut(s)	41, 889, 905, 978, 1216
AjnI	CCWGG	2 cut(s)	510, 1200
AjuI	GAANNNNNNNTTGG	2 cut(s)	695, 727
AleI	CACNNNNGTG	1 cut(s)	310
AluBI	AGCT	7 cut(s)	37, 171, 295, 594, 722, 851, 1082
AluI	AGCT	7 cut(s)	37, 171, 295, 594, 722, 851, 1082
Alw21I	GWGCWC	1 cut(s)	297
Alw26I	GTCTC	2 cut(s)	435, 755
AlwI	GGATC	5 cut(s)	336, 349, 450, 564, 723
AoxI	GGCC	5 cut(s)	57, 63, 352, 375, 962
ApeKI	GCWGC	4 cut(s)	34, 722, 1153, 1163
ApoI	RAATTY	1 cut(s)	327
AspLEI	GCGC	3 cut(s)	453, 479, 937
AsuHPI	GGTGA	4 cut(s)	541, 551, 592, 706
AsuII	TTCGAA	2 cut(s)	458, 1002
BalI	TGGCCA	1 cut(s)	59
BamHI	GGATCC	1 cut(s)	341
BanI	GGYRCC	1 cut(s)	775
BanII	GRGCYC	1 cut(s)	297
BbsI	GAAGAC	3 cut(s)	11, 387, 630
Bbv12I	GWGCWC	1 cut(s)	297
BbvI	GCAGC	4 cut(s)	46, 709, 1165, 1175
BccI	CCATC	2 cut(s)	192, 445
BceAI	ACGGC	3 cut(s)	272, 367, 412
BcgI	CGANNNNNNTGC	4 cut(s)	250, 284, 714, 748
BciT130I	CCWGG	2 cut(s)	512, 1202
BciVI	GTATCC	1 cut(s)	491
BcoDI	GTCTC	2 cut(s)	435, 755
BfaI	CTAG	1 cut(s)	23
BfmI	CTRYAG	3 cut(s)	316, 1095, 1226
BfuI	GTATCC	1 cut(s)	491
BisI	GCNGC	6 cut(s)	35, 723, 785, 1072, 1154, 1164
BlsI	GCNGC	6 cut(s)	36, 724, 786, 1073, 1155, 1165
Bme1390I	CCNGG	2 cut(s)	512, 1202
BmiI	GGNNCC	4 cut(s)	343, 398, 704, 777
BmrFI	CCNGG	2 cut(s)	512, 1202
BmsI	GCATC	3 cut(s)	229, 270, 812
BpiI	GAAGAC	3 cut(s)	11, 387, 630
BplI	GAGNNNNNCTC	2 cut(s)	523, 555
Bpu14I	TTCGAA	2 cut(s)	458, 1002
BpuEI	CTTGAG	2 cut(s)	616, 1191
BsaHI	GRCGYC	1 cut(s)	285
BsaJI	CCNNGG	4 cut(s)	54, 60, 149, 576
BsaWI	WCCGGW	2 cut(s)	132, 1120
Bsc4I	CCNNNNNNNGG	4 cut(s)	79, 582, 712, 1120
Bse3DI	GCAATG	1 cut(s)	738
BseBI	CCWGG	2 cut(s)	512, 1202
BseDI	CCNNGG	4 cut(s)	54, 60, 149, 576
BseGI	GGATG	7 cut(s)	301, 520, 827, 874, 1126, 1150, 1198
BseLI	CCNNNNNNNGG	4 cut(s)	79, 582, 712, 1120
BseMI	GCAATG	1 cut(s)	738
BseMII	CTCAG	2 cut(s)	238, 554
BseRI	GAGGAG	1 cut(s)	113
BseXI	GCAGC	4 cut(s)	46, 709, 1165, 1175
Bsh1236I	CGCG	5 cut(s)	333, 453, 479, 990, 1071
Bsh1285I	CGRYCG	2 cut(s)	137, 158
BshFI	GGCC	5 cut(s)	59, 65, 354, 377, 964
BshNI	GGYRCC	1 cut(s)	775
BsiEI	CGRYCG	2 cut(s)	137, 158
BsiHKAI	GWGCWC	1 cut(s)	297
BsiSI	CCGG	3 cut(s)	77, 133, 1121
BslFI	GGGAC	2 cut(s)	257, 1184
BslI	CCNNNNNNNGG	4 cut(s)	79, 582, 712, 1120
BsmAI	GTCTC	2 cut(s)	435, 755
BsmBI	CGTCTC	1 cut(s)	435
BsmFI	GGGAC	2 cut(s)	257, 1184
BsnI	GGCC	5 cut(s)	59, 65, 354, 377, 964
Bsp119I	TTCGAA	2 cut(s)	458, 1002
Bsp1286I	GDGCHC	1 cut(s)	297
Bsp143I	GATC	4 cut(s)	341, 442, 556, 715
Bsp19I	CCATGG	2 cut(s)	60, 149
BspACI	CCGC	8 cut(s)	74, 118, 127, 154, 675, 785, 988, 1071
BspANI	GGCC	5 cut(s)	59, 65, 354, 377, 964
BspCNI	CTCAG	2 cut(s)	237, 553
BspFNI	CGCG	5 cut(s)	333, 453, 479, 990, 1071
BspLI	GGNNCC	4 cut(s)	343, 398, 704, 777
BspPI	GGATC	5 cut(s)	336, 349, 450, 564, 723
BspT104I	TTCGAA	2 cut(s)	458, 1002
BspT107I	GGYRCC	1 cut(s)	775
BsrBI	CCGCTC	1 cut(s)	675
BsrDI	GCAATG	1 cut(s)	738
BssECI	CCNNGG	4 cut(s)	54, 60, 149, 576
BssMI	GATC	4 cut(s)	341, 442, 556, 715
BssNI	GRCGYC	1 cut(s)	285
BssT1I	CCWWGG	2 cut(s)	60, 149
Bst2UI	CCWGG	2 cut(s)	512, 1202
Bst4CI	ACNGT	2 cut(s)	55, 931
Bst6I	CTCTTC	1 cut(s)	1181
BstACI	GRCGYC	1 cut(s)	285
BstBI	TTCGAA	2 cut(s)	458, 1002
BstC8I	GCNNGC	3 cut(s)	118, 1076, 1080
BstDEI	CTNAG	2 cut(s)	224, 540
BstDSI	CCRYGG	3 cut(s)	54, 60, 149
BstF5I	GGATG	7 cut(s)	301, 520, 827, 874, 1126, 1150, 1198
BstFNI	CGCG	5 cut(s)	333, 453, 479, 990, 1071
BstHHI	GCGC	3 cut(s)	453, 479, 937
BstKTI	GATC	4 cut(s)	344, 445, 559, 718
BstMAI	GTCTC	2 cut(s)	435, 755
BstMBI	GATC	4 cut(s)	341, 442, 556, 715
BstMCI	CGRYCG	2 cut(s)	137, 158
BstMWI	GCNNNNNNNGC	6 cut(s)	56, 126, 360, 433, 474, 784
BstNI	CCWGG	2 cut(s)	512, 1202
BstNSI	RCATGY	2 cut(s)	466, 631
BstSCI	CCNGG	2 cut(s)	510, 1200
BstSFI	CTRYAG	3 cut(s)	316, 1095, 1226
BstUI	CGCG	5 cut(s)	333, 453, 479, 990, 1071
BstV1I	GCAGC	4 cut(s)	46, 709, 1165, 1175
BstV2I	GAAGAC	3 cut(s)	11, 387, 630
BstX2I	RGATCY	1 cut(s)	341
BstYI	RGATCY	1 cut(s)	341
BsuI	GTATCC	1 cut(s)	491
BsuRI	GGCC	5 cut(s)	59, 65, 354, 377, 964
BtgI	CCRYGG	3 cut(s)	54, 60, 149
BtgZI	GCGATG	1 cut(s)	370
BtsCI	GGATG	7 cut(s)	301, 520, 827, 874, 1126, 1150, 1198
Cac8I	GCNNGC	3 cut(s)	118, 1076, 1080
CfoI	GCGC	3 cut(s)	453, 479, 937
CseI	GACGC	2 cut(s)	40, 293
Csp6I	GTAC	2 cut(s)	415, 638
CviAII	CATG	6 cut(s)	61, 150, 310, 463, 628, 793
CviQI	GTAC	2 cut(s)	415, 638
DdeI	CTNAG	2 cut(s)	224, 540
DpnI	GATC	4 cut(s)	343, 444, 558, 717
DpnII	GATC	4 cut(s)	341, 442, 556, 715
EaeI	YGGCCR	3 cut(s)	57, 63, 375
Eam1104I	CTCTTC	1 cut(s)	1181
EarI	CTCTTC	1 cut(s)	1181
Ecl136II	GAGCTC	1 cut(s)	295
Eco130I	CCWWGG	2 cut(s)	60, 149
Eco147I	AGGCCT	1 cut(s)	964
Eco24I	GRGCYC	1 cut(s)	297
Eco53kI	GAGCTC	1 cut(s)	295
EcoICRI	GAGCTC	1 cut(s)	295
EcoRI	GAATTC	1 cut(s)	327
EcoRII	CCWGG	2 cut(s)	510, 1200
EcoT14I	CCWWGG	2 cut(s)	60, 149
EcoT38I	GRGCYC	1 cut(s)	297
ErhI	CCWWGG	2 cut(s)	60, 149
Esp3I	CGTCTC	1 cut(s)	435
FaeI	CATG	6 cut(s)	64, 153, 313, 466, 631, 796
FaqI	GGGAC	2 cut(s)	257, 1184
FatI	CATG	6 cut(s)	60, 149, 309, 462, 627, 792
FauI	CCCGC	2 cut(s)	81, 125
FblI	GTMKAC	3 cut(s)	90, 404, 1135
Fnu4HI	GCNGC	6 cut(s)	35, 723, 785, 1072, 1154, 1164
FokI	GGATG	7 cut(s)	288, 527, 834, 881, 1113, 1157, 1205
FriOI	GRGCYC	1 cut(s)	297
Fsp4HI	GCNGC	6 cut(s)	35, 723, 785, 1072, 1154, 1164
FspBI	CTAG	1 cut(s)	23
FspI	TGCGCA	1 cut(s)	936
GlaI	GCGC	3 cut(s)	452, 478, 936
GluI	GCNGC	6 cut(s)	35, 723, 785, 1072, 1154, 1164
HaeIII	GGCC	5 cut(s)	59, 65, 354, 377, 964
HapII	CCGG	3 cut(s)	77, 133, 1121
HgaI	GACGC	2 cut(s)	40, 293
HhaI	GCGC	3 cut(s)	453, 479, 937
Hin1I	GRCGYC	1 cut(s)	285
Hin1II	CATG	6 cut(s)	64, 153, 313, 466, 631, 796
Hin6I	GCGC	3 cut(s)	451, 477, 935
HinP1I	GCGC	3 cut(s)	451, 477, 935
HincII	GTYRAC	2 cut(s)	91, 405
HindII	GTYRAC	2 cut(s)	91, 405
HpaII	CCGG	3 cut(s)	77, 133, 1121
HphI	GGTGA	4 cut(s)	541, 551, 592, 706
Hpy166II	GTNNAC	5 cut(s)	91, 405, 882, 927, 1136
Hpy188I	TCNGA	6 cut(s)	250, 442, 551, 577, 702, 810
Hpy188III	TCNNGA	4 cut(s)	394, 770, 905, 952
Hpy8I	GTNNAC	5 cut(s)	91, 405, 882, 927, 1136
Hpy99I	CGWCG	7 cut(s)	34, 92, 116, 287, 293, 406, 409
HpyAV	CCTTC	3 cut(s)	365, 883, 1042
HpyCH4III	ACNGT	2 cut(s)	55, 931
HpyCH4IV	ACGT	2 cut(s)	372, 750
HpyCH4V	TGCA	7 cut(s)	143, 261, 348, 685, 725, 743, 843
HpyF10VI	GCNNNNNNNGC	6 cut(s)	56, 126, 360, 433, 474, 784
HpyF3I	CTNAG	2 cut(s)	224, 540
HpySE526I	ACGT	2 cut(s)	372, 750
Hsp92I	GRCGYC	1 cut(s)	285
Hsp92II	CATG	6 cut(s)	64, 153, 313, 466, 631, 796
HspAI	GCGC	3 cut(s)	451, 477, 935
Kzo9I	GATC	4 cut(s)	341, 442, 556, 715
Lsp1109I	GCAGC	4 cut(s)	46, 709, 1165, 1175
LweI	GCATC	3 cut(s)	229, 270, 812
MaeI	CTAG	1 cut(s)	23
MaeII	ACGT	2 cut(s)	372, 750
MaeIII	GTNAC	3 cut(s)	368, 644, 694
MalI	GATC	4 cut(s)	343, 444, 558, 717
MbiI	CCGCTC	1 cut(s)	675
MboI	GATC	4 cut(s)	341, 442, 556, 715
MboII	GAAGA	7 cut(s)	16, 392, 421, 635, 872, 957, 1198
MflI	RGATCY	1 cut(s)	341
MhlI	GDGCHC	1 cut(s)	297
MlsI	TGGCCA	1 cut(s)	59
MluCI	AATT	5 cut(s)	327, 524, 710, 920, 1217
MluNI	TGGCCA	1 cut(s)	59
MlyI	GAGTC	6 cut(s)	218, 239, 266, 627, 691, 917
MmeI	TCCRAC	1 cut(s)	273
Mox20I	TGGCCA	1 cut(s)	59
MscI	TGGCCA	1 cut(s)	59
MseI	TTAA	2 cut(s)	278, 1062
MslI	CAYNNNNRTG	1 cut(s)	310
Msp20I	TGGCCA	1 cut(s)	59
MspI	CCGG	3 cut(s)	77, 133, 1121
MspR9I	CCNGG	2 cut(s)	512, 1202
MvaI	CCWGG	2 cut(s)	512, 1202
MvnI	CGCG	5 cut(s)	333, 453, 479, 990, 1071
MwoI	GCNNNNNNNGC	6 cut(s)	56, 126, 360, 433, 474, 784
NcoI	CCATGG	2 cut(s)	60, 149
NdeII	GATC	4 cut(s)	341, 442, 556, 715
NlaIII	CATG	6 cut(s)	64, 153, 313, 466, 631, 796
NlaIV	GGNNCC	4 cut(s)	343, 398, 704, 777
NmuCI	GTSAC	1 cut(s)	694
NsbI	TGCGCA	1 cut(s)	936
NspI	RCATGY	2 cut(s)	466, 631
NspV	TTCGAA	2 cut(s)	458, 1002
OliI	CACNNNNGTG	1 cut(s)	310
PceI	AGGCCT	1 cut(s)	964
PciI	ACATGT	1 cut(s)	462
PcsI	WCGNNNNNNNCGW	1 cut(s)	288
PfeI	GAWTC	6 cut(s)	232, 420, 455, 664, 731, 948
PflFI	GACNNNGTC	1 cut(s)	287
PfoI	TCCNGGA	1 cut(s)	510
PkrI	GCNGC	6 cut(s)	36, 724, 786, 1073, 1155, 1165
PleI	GAGTC	6 cut(s)	217, 239, 266, 626, 691, 916
PpsI	GAGTC	6 cut(s)	217, 239, 266, 626, 691, 916
PscI	ACATGT	1 cut(s)	462
Psp124BI	GAGCTC	1 cut(s)	297
Psp6I	CCWGG	2 cut(s)	510, 1200
PspGI	CCWGG	2 cut(s)	510, 1200
PspN4I	GGNNCC	4 cut(s)	343, 398, 704, 777
PsuI	RGATCY	1 cut(s)	341
PsyI	GACNNNGTC	1 cut(s)	287
RsaI	GTAC	2 cut(s)	416, 639
RsaNI	GTAC	2 cut(s)	415, 638
RseI	CAYNNNNRTG	1 cut(s)	310
SacI	GAGCTC	1 cut(s)	297
SalI	GTCGAC	2 cut(s)	89, 403
SaqAI	TTAA	2 cut(s)	278, 1062
SatI	GCNGC	6 cut(s)	35, 723, 785, 1072, 1154, 1164
Sau3AI	GATC	4 cut(s)	341, 442, 556, 715
SchI	GAGTC	6 cut(s)	218, 239, 266, 627, 691, 917
ScrFI	CCNGG	2 cut(s)	512, 1202
SduI	GDGCHC	1 cut(s)	297
SfaNI	GCATC	3 cut(s)	229, 270, 812
SfcI	CTRYAG	3 cut(s)	316, 1095, 1226
SfuI	TTCGAA	2 cut(s)	458, 1002
SgrDI	CGTCGACG	1 cut(s)	403
SmiMI	CAYNNNNRTG	1 cut(s)	310
SmlI	CTYRAG	2 cut(s)	595, 1206
SmoI	CTYRAG	2 cut(s)	595, 1206
Sse9I	AATT	5 cut(s)	327, 524, 710, 920, 1217
SseBI	AGGCCT	1 cut(s)	964
SsiI	CCGC	8 cut(s)	74, 118, 127, 154, 675, 785, 988, 1071
SspMI	CTAG	1 cut(s)	23
SstI	GAGCTC	1 cut(s)	297
StuI	AGGCCT	1 cut(s)	964
StyD4I	CCNGG	2 cut(s)	510, 1200
StyI	CCWWGG	2 cut(s)	60, 149
TaaI	ACNGT	2 cut(s)	55, 931
TaiI	ACGT	2 cut(s)	375, 753
TaqII	GACCGA	1 cut(s)	724
TasI	AATT	5 cut(s)	327, 524, 710, 920, 1217
TatI	WGTACW	2 cut(s)	414, 637
TauI	GCSGC	2 cut(s)	787, 1074
TfiI	GAWTC	6 cut(s)	232, 420, 455, 664, 731, 948
Tru1I	TTAA	2 cut(s)	278, 1062
Tru9I	TTAA	2 cut(s)	278, 1062
TseFI	GTSAC	1 cut(s)	694
TseI	GCWGC	4 cut(s)	34, 722, 1153, 1163
Tsp45I	GTSAC	1 cut(s)	694
TspDTI	ATGAA	4 cut(s)	17, 560, 873, 1115
Tth111I	GACNNNGTC	1 cut(s)	287
XapI	RAATTY	1 cut(s)	327
XceI	RCATGY	2 cut(s)	466, 631
XmiI	GTMKAC	3 cut(s)	90, 404, 1135
XspI	CTAG	1 cut(s)	23

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.