pycom10g28200

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004460
Physical Location & Seq
Reverse (-)
28883019 .. 28884435
1417 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g28200.1

Sequence Viewer

Length: 1230 bp
ATGAAGACTCCAAAATCCACCCTAGATACGACGCAGCTTCAAATCCAAGCATCCATGGCCATGGCCGAACTCCCGCCGGAGGTAATCGTCGACATACTCTCTCGTCTGCCCGTCGCCCGCCTCCTCCTCTTCCGGTCGATTGCAAAACCATGGCGGTCGCTCATCGACAGCTCATACTTCGTCCATCTCCACCTCACGCAATCGGCAGAGTCCAAATCGCATCTCAGTTTGATTCTCAGAAAGGAATCTGACCTCTACTGCATCAGTTTCGACTCCTTAAACGACGCCGTCGAGCTCAATCATCCACTCATGTGCTACAGCAATCGAATTCGCGTTTTGGGATCCTGCAACGGCCTTCTGTGCCTCTGTAACGTGGCGGAAGACATCGCTCTCTGGAACCCGTCGACGAAGAAGTACAGAATCTTGCCGTCTCTGCCATCGGATCGAAAGCTCGATTCGAACATGTGCTTATGTGGCGCGAGGGTATATGGTCTTGGATACGACGCTATTCAGGATGACTATAAATTGGTGAGGATTTCTCAGTTTATCGGATTGGATTACCTGTCGTTTCAGTCCGAGGTGAGGGTTTATAGCCTGAGAAGCAACGAGTGGAAGCGACTCGAAGACATGCCTTATGTACTCTGTTACACACGAAAAATGGGGATTCTTGTGAGCGGTTGTGTGCATTGGGTGGTGACTCGAAACCTCGAATTGGATCAATCAGCAACCGAATTGGTCATTGCATTTGACGTTGCGAATGAGACTTTTCGTGAGGTGCCTATGCCGCAGAACATGGACAGTAAGTGTCAGATTGATGTGGGATGCTTGGGAGGGTGTCTTTGCATTGTAGCTAAATATGAAGATAAGGATGTTGATGTGTGGACATTGAAGGATTATGGGGTTAAAGAGTCTTGGAGTAAATTGTTTACCGTTGCGCAGACTCGAAGAATCAAGAGCGTTAGGCCTTTGGTGTATTCAAAGAATGACCGCGAAGTTTTGTTCGAACAGGACCACGAATGTCTTGTTTGGTTTGACCTGAAAAGCCAGAGGGTTAAGCATGTCAAGGTTCGCGGCTTGCCAGATTTGTTTGAGGCTGTAGTTTGTATGGAAAGCCTTGTTTCGGTTCATCCTCGTAGACAAGACGATCGTAAGCAGCAGGAAGCAGCAGGGGACAAGAAGAATAAGAAGAGGGATGATTTTCTGGCCCAAGGCTTCAAATTGGTGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

410

Amino Acids

46.91

Weight (kDa)

8.09

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 23 - 61 5e-06 F-box domain
FBA_1 PF07734 78 - 351 1.6e-28 F-box associated beta propeller domain
FBA_3 PF08268 78 - 362 3.6e-26 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 108 - 354 7.2e-10 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 936
AccB1I GGYRCC 1 cut(s) 775
AccBSI CCGCTC 1 cut(s) 675
AccI GTMKAC 3 cut(s) 90, 404, 1135
AccII CGCG 4 cut(s) 333, 479, 990, 1071
AciI CCGC 8 cut(s) 74, 118, 154, 377, 675, 785, 988, 1071
AclWI GGATC 4 cut(s) 336, 349, 450, 723
AcoI YGGCCR 2 cut(s) 57, 63
AcsI RAATTY 1 cut(s) 327
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 2 cut(s) 416, 639
AfiI CCNNNNNNNGG 4 cut(s) 79, 582, 712, 1120
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 4 cut(s) 41, 889, 978, 1216
AleI CACNNNNGTG 1 cut(s) 310
AluBI AGCT 5 cut(s) 37, 171, 295, 451, 851
AluI AGCT 5 cut(s) 37, 171, 295, 451, 851
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 435, 755
AlwI GGATC 4 cut(s) 336, 349, 450, 723
AoxI GGCC 5 cut(s) 57, 63, 352, 962, 1203
ApeKI GCWGC 3 cut(s) 34, 1153, 1163
ApoI RAATTY 1 cut(s) 327
AspLEI GCGC 2 cut(s) 479, 937
AspS9I GGNCC 2 cut(s) 1009, 1204
AsuHPI GGTGA 3 cut(s) 541, 592, 706
AsuII TTCGAA 2 cut(s) 458, 1002
AvaII GGWCC 1 cut(s) 1009
BalI TGGCCA 1 cut(s) 59
BamHI GGATCC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 775
BanII GRGCYC 1 cut(s) 297
BbsI GAAGAC 3 cut(s) 11, 387, 630
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 3 cut(s) 46, 1165, 1175
BccI CCATC 2 cut(s) 192, 445
BceAI ACGGC 3 cut(s) 272, 367, 412
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciVI GTATCC 1 cut(s) 491
BcoDI GTCTC 2 cut(s) 435, 755
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 3 cut(s) 316, 1095, 1226
BfuI GTATCC 1 cut(s) 491
BisI GCNGC 5 cut(s) 35, 785, 1072, 1154, 1164
BlsI GCNGC 5 cut(s) 36, 786, 1073, 1155, 1165
Bme18I GGWCC 1 cut(s) 1009
BmgT120I GGNCC 2 cut(s) 1009, 1204
BmiI GGNNCC 3 cut(s) 343, 398, 777
BmsI GCATC 4 cut(s) 59, 229, 270, 812
BpiI GAAGAC 3 cut(s) 11, 387, 630
BplI GAGNNNNNCTC 2 cut(s) 523, 555
Bpu14I TTCGAA 2 cut(s) 458, 1002
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 5 cut(s) 54, 60, 149, 576, 1207
BsaWI WCCGGW 1 cut(s) 132
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 582, 712, 1120
Bse3DI GCAATG 1 cut(s) 738
BseDI CCNNGG 5 cut(s) 54, 60, 149, 576, 1207
BseGI GGATG 7 cut(s) 50, 301, 520, 827, 874, 1126, 1198
BseLI CCNNNNNNNGG 4 cut(s) 79, 582, 712, 1120
BseMI GCAATG 1 cut(s) 738
BseMII CTCAG 4 cut(s) 238, 250, 554, 587
BseRI GAGGAG 2 cut(s) 113, 116
BseXI GCAGC 3 cut(s) 46, 1165, 1175
Bsh1236I CGCG 4 cut(s) 333, 479, 990, 1071
Bsh1285I CGRYCG 3 cut(s) 137, 158, 1147
BshFI GGCC 5 cut(s) 59, 65, 354, 964, 1205
BshNI GGYRCC 1 cut(s) 775
BsiEI CGRYCG 3 cut(s) 137, 158, 1147
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 2 cut(s) 77, 133
BslFI GGGAC 1 cut(s) 1184
BslI CCNNNNNNNGG 4 cut(s) 79, 582, 712, 1120
BsmAI GTCTC 2 cut(s) 435, 755
BsmBI CGTCTC 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 1184
BsnI GGCC 5 cut(s) 59, 65, 354, 964, 1205
Bsp119I TTCGAA 2 cut(s) 458, 1002
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 4 cut(s) 341, 442, 715, 1144
Bsp19I CCATGG 3 cut(s) 54, 60, 149
BspACI CCGC 8 cut(s) 74, 118, 154, 377, 675, 785, 988, 1071
BspANI GGCC 5 cut(s) 59, 65, 354, 964, 1205
BspCNI CTCAG 4 cut(s) 237, 249, 553, 588
BspFNI CGCG 4 cut(s) 333, 479, 990, 1071
BspLI GGNNCC 3 cut(s) 343, 398, 777
BspPI GGATC 4 cut(s) 336, 349, 450, 723
BspT104I TTCGAA 2 cut(s) 458, 1002
BspT107I GGYRCC 1 cut(s) 775
BsrBI CCGCTC 1 cut(s) 675
BsrDI GCAATG 1 cut(s) 738
BssECI CCNNGG 5 cut(s) 54, 60, 149, 576, 1207
BssMI GATC 4 cut(s) 341, 442, 715, 1144
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 4 cut(s) 54, 60, 149, 1207
Bst4CI ACNGT 2 cut(s) 800, 931
Bst6I CTCTTC 2 cut(s) 134, 1181
BstACI GRCGYC 1 cut(s) 285
BstBI TTCGAA 2 cut(s) 458, 1002
BstC8I GCNNGC 2 cut(s) 118, 1076
BstDEI CTNAG 4 cut(s) 224, 236, 540, 596
BstDSI CCRYGG 3 cut(s) 54, 60, 149
BstF5I GGATG 7 cut(s) 50, 301, 520, 827, 874, 1126, 1198
BstFNI CGCG 4 cut(s) 333, 479, 990, 1071
BstHHI GCGC 2 cut(s) 479, 937
BstKTI GATC 4 cut(s) 344, 445, 718, 1147
BstMAI GTCTC 2 cut(s) 435, 755
BstMBI GATC 4 cut(s) 341, 442, 715, 1144
BstMCI CGRYCG 3 cut(s) 137, 158, 1147
BstMWI GCNNNNNNNGC 6 cut(s) 56, 360, 433, 474, 600, 784
BstNSI RCATGY 3 cut(s) 466, 631, 1061
BstSFI CTRYAG 3 cut(s) 316, 1095, 1226
BstUI CGCG 4 cut(s) 333, 479, 990, 1071
BstV1I GCAGC 3 cut(s) 46, 1165, 1175
BstV2I GAAGAC 3 cut(s) 11, 387, 630
BstX2I RGATCY 1 cut(s) 341
BstXI CCANNNNNNTGG 1 cut(s) 61
BstYI RGATCY 1 cut(s) 341
BsuI GTATCC 1 cut(s) 491
BsuRI GGCC 5 cut(s) 59, 65, 354, 964, 1205
BtgI CCRYGG 3 cut(s) 54, 60, 149
BtgZI GCGATG 1 cut(s) 370
BtsCI GGATG 7 cut(s) 50, 301, 520, 827, 874, 1126, 1198
Cac8I GCNNGC 2 cut(s) 118, 1076
CfoI GCGC 2 cut(s) 479, 937
Cfr13I GGNCC 2 cut(s) 1009, 1204
CseI GACGC 3 cut(s) 40, 293, 512
Csp6I GTAC 2 cut(s) 415, 638
CviAII CATG 8 cut(s) 55, 61, 150, 310, 463, 628, 793, 1058
CviQI GTAC 2 cut(s) 415, 638
DdeI CTNAG 4 cut(s) 224, 236, 540, 596
DpnI GATC 4 cut(s) 343, 444, 717, 1146
DpnII GATC 4 cut(s) 341, 442, 715, 1144
EaeI YGGCCR 2 cut(s) 57, 63
Eam1104I CTCTTC 2 cut(s) 134, 1181
EarI CTCTTC 2 cut(s) 134, 1181
EciI GGCGGA 1 cut(s) 392
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 4 cut(s) 54, 60, 149, 1207
Eco147I AGGCCT 1 cut(s) 964
Eco24I GRGCYC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 1009
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 327
EcoT14I CCWWGG 4 cut(s) 54, 60, 149, 1207
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 4 cut(s) 54, 60, 149, 1207
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 8 cut(s) 58, 64, 153, 313, 466, 631, 796, 1061
FaqI GGGAC 1 cut(s) 1184
FatI CATG 8 cut(s) 54, 60, 149, 309, 462, 627, 792, 1057
FauI CCCGC 2 cut(s) 81, 125
FblI GTMKAC 3 cut(s) 90, 404, 1135
Fnu4HI GCNGC 5 cut(s) 35, 785, 1072, 1154, 1164
FokI GGATG 7 cut(s) 37, 288, 527, 834, 881, 1113, 1205
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 5 cut(s) 35, 785, 1072, 1154, 1164
FspBI CTAG 1 cut(s) 23
FspI TGCGCA 1 cut(s) 936
GlaI GCGC 2 cut(s) 478, 936
GluI GCNGC 5 cut(s) 35, 785, 1072, 1154, 1164
HaeIII GGCC 5 cut(s) 59, 65, 354, 964, 1205
HapII CCGG 2 cut(s) 77, 133
HgaI GACGC 3 cut(s) 40, 293, 512
HhaI GCGC 2 cut(s) 479, 937
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 8 cut(s) 58, 64, 153, 313, 466, 631, 796, 1061
Hin6I GCGC 2 cut(s) 477, 935
HinP1I GCGC 2 cut(s) 477, 935
HincII GTYRAC 2 cut(s) 91, 405
HindII GTYRAC 2 cut(s) 91, 405
HpaII CCGG 2 cut(s) 77, 133
HphI GGTGA 3 cut(s) 541, 592, 706
Hpy166II GTNNAC 5 cut(s) 91, 405, 882, 927, 1136
Hpy188I TCNGA 6 cut(s) 239, 250, 442, 551, 577, 810
Hpy188III TCNNGA 4 cut(s) 394, 512, 770, 952
Hpy8I GTNNAC 5 cut(s) 91, 405, 882, 927, 1136
Hpy99I CGWCG 8 cut(s) 34, 92, 116, 287, 293, 406, 409, 506
HpyAV CCTTC 2 cut(s) 365, 883
HpyCH4III ACNGT 2 cut(s) 800, 931
HpyCH4IV ACGT 2 cut(s) 372, 750
HpyCH4V TGCA 6 cut(s) 143, 261, 348, 685, 743, 843
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 360, 433, 474, 600, 784
HpyF3I CTNAG 4 cut(s) 224, 236, 540, 596
HpySE526I ACGT 2 cut(s) 372, 750
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 8 cut(s) 58, 64, 153, 313, 466, 631, 796, 1061
HspAI GCGC 2 cut(s) 477, 935
Kzo9I GATC 4 cut(s) 341, 442, 715, 1144
Lsp1109I GCAGC 3 cut(s) 46, 1165, 1175
LweI GCATC 4 cut(s) 59, 229, 270, 812
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 372, 750
MaeIII GTNAC 3 cut(s) 368, 644, 694
MalI GATC 4 cut(s) 343, 444, 717, 1146
MbiI CCGCTC 1 cut(s) 675
MboI GATC 4 cut(s) 341, 442, 715, 1144
MboII GAAGA 9 cut(s) 16, 121, 392, 421, 635, 872, 957, 1189, 1198
MflI RGATCY 1 cut(s) 341
MhlI GDGCHC 1 cut(s) 297
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 6 cut(s) 327, 524, 710, 731, 920, 1217
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 6 cut(s) 218, 266, 612, 691, 917, 934
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MseI TTAA 3 cut(s) 278, 903, 1053
MslI CAYNNNNRTG 2 cut(s) 59, 310
Msp20I TGGCCA 1 cut(s) 59
MspI CCGG 2 cut(s) 77, 133
MvnI CGCG 4 cut(s) 333, 479, 990, 1071
MwoI GCNNNNNNNGC 6 cut(s) 56, 360, 433, 474, 600, 784
NcoI CCATGG 3 cut(s) 54, 60, 149
NdeII GATC 4 cut(s) 341, 442, 715, 1144
NlaIII CATG 8 cut(s) 58, 64, 153, 313, 466, 631, 796, 1061
NlaIV GGNNCC 3 cut(s) 343, 398, 777
NmuCI GTSAC 1 cut(s) 694
NsbI TGCGCA 1 cut(s) 936
NspI RCATGY 3 cut(s) 466, 631, 1061
NspV TTCGAA 2 cut(s) 458, 1002
OliI CACNNNNGTG 1 cut(s) 310
PceI AGGCCT 1 cut(s) 964
PciI ACATGT 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PfeI GAWTC 6 cut(s) 232, 245, 420, 455, 664, 948
PflFI GACNNNGTC 1 cut(s) 287
PkrI GCNGC 5 cut(s) 36, 786, 1073, 1155, 1165
Ple19I CGATCG 1 cut(s) 1147
PleI GAGTC 6 cut(s) 217, 266, 612, 691, 916, 934
PpsI GAGTC 6 cut(s) 217, 266, 612, 691, 916, 934
PscI ACATGT 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 343, 398, 777
PspPI GGNCC 2 cut(s) 1009, 1204
PsuI RGATCY 1 cut(s) 341
PsyI GACNNNGTC 1 cut(s) 287
PvuI CGATCG 1 cut(s) 1147
RsaI GTAC 2 cut(s) 416, 639
RsaNI GTAC 2 cut(s) 415, 638
RseI CAYNNNNRTG 2 cut(s) 59, 310
SacI GAGCTC 1 cut(s) 297
SalI GTCGAC 2 cut(s) 89, 403
SaqAI TTAA 3 cut(s) 278, 903, 1053
SatI GCNGC 5 cut(s) 35, 785, 1072, 1154, 1164
Sau3AI GATC 4 cut(s) 341, 442, 715, 1144
Sau96I GGNCC 2 cut(s) 1009, 1204
SchI GAGTC 6 cut(s) 218, 266, 612, 691, 917, 934
SduI GDGCHC 1 cut(s) 297
SfaNI GCATC 4 cut(s) 59, 229, 270, 812
SfcI CTRYAG 3 cut(s) 316, 1095, 1226
SfuI TTCGAA 2 cut(s) 458, 1002
SgrDI CGTCGACG 1 cut(s) 403
SinI GGWCC 1 cut(s) 1009
SmiMI CAYNNNNRTG 2 cut(s) 59, 310
Sse9I AATT 6 cut(s) 327, 524, 710, 731, 920, 1217
SseBI AGGCCT 1 cut(s) 964
SsiI CCGC 8 cut(s) 74, 118, 154, 377, 675, 785, 988, 1071
SspMI CTAG 1 cut(s) 23
SstI GAGCTC 1 cut(s) 297
StuI AGGCCT 1 cut(s) 964
StyI CCWWGG 4 cut(s) 54, 60, 149, 1207
TaaI ACNGT 2 cut(s) 800, 931
TaiI ACGT 2 cut(s) 375, 753
TasI AATT 6 cut(s) 327, 524, 710, 731, 920, 1217
TatI WGTACW 2 cut(s) 414, 637
TauI GCSGC 2 cut(s) 787, 1074
TfiI GAWTC 6 cut(s) 232, 245, 420, 455, 664, 948
Tru1I TTAA 3 cut(s) 278, 903, 1053
Tru9I TTAA 3 cut(s) 278, 903, 1053
TseFI GTSAC 1 cut(s) 694
TseI GCWGC 3 cut(s) 34, 1153, 1163
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 3 cut(s) 17, 873, 1115
Tth111I GACNNNGTC 1 cut(s) 287
VpaK11BI GGWCC 1 cut(s) 1009
XapI RAATTY 1 cut(s) 327
XceI RCATGY 3 cut(s) 466, 631, 1061
XmiI GTMKAC 3 cut(s) 90, 404, 1135
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.