Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003241

Physical Location & Seq

Genomic Coordinates Reverse (-)

41455332 .. 41466218

Gene Structure 10887 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1338400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 861 bp

ATGGGGAATGTGAATGGGAGAGAAGATGGGGATGGAAGCCCCTCAGCGGCCGAGGAAGATAGCGGTGCCGCCGGCAGCTTGCAGGAGGGCCCTGCCCACGGCGCCCGTCTTGTTGATGGGTCCGCTGAGTTGATGGGTCAATCGCCTCCTCATAGCCCTAGGGCCACTCACTCCCCTTTGATGTTCACTCCCCAAGTCCCTGTCGTTCCCTTACAAAGACCAGATGAGATACAAATTCCAAACAGCTCGTGGATGCAAACGTCGGGGTTTGAAGACATGTGCTGTGAGCAAGGAATTCCAACTATGATTACATGGAGCTATGGTGGGAAGGATGTAGCTGTGGAGGGATCATGGGATAATTGGAAGACAAGATTGGCTTTGCAGAGATCAGGGAAGGACTTCACTATTATGAAGGTACTGCCATCTGGTGTTTACCAATATAGGTTTATTGTTGATGGGCAGTGGAGATGTTCTCCTGACTTGCCCTTGACCCAAGATGATGCTGGCAATTCTTACAACCTTTTGGACTTGCAGGATTACGTCCCAGAAGACATCGGCAGCATTTCTGGTTTTGAACCTCCCCAGTCGCCGGAATCAAGTTATAACAACCTGCAGCTTGGATCTGAAGATTTCGCGAAGGAGCCCCCATTGGTTCCTCCACACCTACAAATGACACTGCTGAATGCACCTTCATCTTACATGGAGATGCCGCCTCCTTTGTCAAGACCTCAACATGTGGTACTCAATCATCTTTACATGCAGAGAGGGAAGAGTGGACCATCTGTGGTGGCACTCGGTACAACAGAGAGGTTTATAGCGAAGTATGTTACAGTGGTTCTCTACAAGTCCTTGCAGAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04068 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 map04068 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410

Pfam Domains

Protein Families

AMPK1_CBM AMPKBI

Protein Analysis

287

Amino Acids

31.33

Weight (kDa)

4.81

Isoelectric Point (pI)

58.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMPK1_CBM	PF16561	99 - 178	6.7e-33	Glycogen recognition site of AMP-activated protein kinase
AMPKBI	PF04739	211 - 283	1.3e-25	5'-AMP-activated protein kinase beta subunit, interaction domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003241)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21170 AT5G21170 AT5G21170
fragaria_vesca	FvH4_1g28790 FvH4_1g28790 FvH4_3g00390
malus_domestica	MD05G1360800.v1.1 MD10G1338400.v1.1 MD15G1312500.v1.1
prunus_persica	Prupe.4G004500_v2.0.a1
pyrus_communis	pycom05g32760 pycom10g28820
rosa_chinensis	RchiOBHm_Chr5g0000591 RchiOBHm_Chr5g0050101
rosa_laevigata	RLG00000030878 RLG00000034686
rosa_multiflora	Rmu_sc0002289.1_g000010 Rmu_sc0003699.1_g000005
rosa_roxburghii	Rroxscaffold_1G00031050 Rroxscaffold_1G00075620
rosa_rugosa	Rorug04G0385100 Rorug04G0385100 Rorug04G0385200 Rorug04G0385300.1 Rorug05G0258500 Rorug05G0258600 Rorug05G0258700 Rorug05G0258800
rosa_samantha	Rh5AG004800 Rh5AG330500 Rh5BG006400 Rh5BG341200 Rh5CG005200 Rh5DG005200 Rh5DG354000
rosa_wichuraiana	Rw5G000480 Rw5G031220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	603
Acc36I	ACCTGC	1 cut(s)	618
AccB1I	GGYRCC	2 cut(s)	65, 101
AccII	CGCG	1 cut(s)	635
AciI	CCGC	5 cut(s)	47, 63, 69, 123, 710
AclWI	GGATC	2 cut(s)	355, 628
AcoI	YGGCCR	1 cut(s)	48
AcsI	RAATTY	2 cut(s)	234, 294
AcuI	CTGAAG	1 cut(s)	645
AcyI	GRCGYC	1 cut(s)	102
AfaI	GTAC	3 cut(s)	417, 741, 799
AfiI	CCNNNNNNNGG	3 cut(s)	46, 98, 589
AflIII	ACRYGT	2 cut(s)	276, 733
AgsI	TTSAA	2 cut(s)	272, 575
AjuI	GAANNNNNNNTTGG	2 cut(s)	356, 388
AluBI	AGCT	5 cut(s)	78, 246, 318, 338, 616
AluI	AGCT	5 cut(s)	78, 246, 318, 338, 616
AlwI	GGATC	2 cut(s)	355, 628
AoxI	GGCC	3 cut(s)	48, 88, 162
ApaI	GGGCCC	1 cut(s)	92
ApeKI	GCWGC	3 cut(s)	75, 558, 613
ApoI	RAATTY	2 cut(s)	234, 294
Asp700I	GAANNNNTTC	1 cut(s)	398
AspA2I	CCTAGG	1 cut(s)	158
AspLEI	GCGC	1 cut(s)	104
AspS9I	GGNCC	5 cut(s)	88, 89, 120, 162, 776
AvaII	GGWCC	2 cut(s)	120, 776
AvrII	CCTAGG	1 cut(s)	158
BaeGI	GKGCMC	1 cut(s)	92
BanI	GGYRCC	2 cut(s)	65, 101
BanII	GRGCYC	2 cut(s)	92, 645
BauI	CACGAG	1 cut(s)	247
BbsI	GAAGAC	3 cut(s)	279, 371, 555
BbvCI	CCTCAGC	1 cut(s)	43
BbvI	GCAGC	3 cut(s)	87, 570, 625
BccI	CCATC	7 cut(s)	20, 26, 110, 127, 430, 449, 787
BceAI	ACGGC	1 cut(s)	115
BfaI	CTAG	1 cut(s)	159
BfmI	CTRYAG	1 cut(s)	611
BfoI	RGCGCY	1 cut(s)	105
BfuAI	ACCTGC	1 cut(s)	618
BisI	GCNGC	6 cut(s)	48, 69, 76, 559, 614, 710
BlnI	CCTAGG	1 cut(s)	158
BlsI	GCNGC	6 cut(s)	49, 70, 77, 560, 615, 711
Bme18I	GGWCC	2 cut(s)	120, 776
BmgT120I	GGNCC	5 cut(s)	88, 89, 120, 162, 776
BmiI	GGNNCC	6 cut(s)	67, 90, 103, 121, 642, 654
BmrI	ACTGGG	1 cut(s)	577
BmsI	GCATC	3 cut(s)	243, 490, 696
BmuI	ACTGGG	1 cut(s)	577
BpiI	GAAGAC	3 cut(s)	279, 371, 555
BplI	GAGNNNNNCTC	2 cut(s)	457, 489
Bpu10I	CCTNAGC	1 cut(s)	43
BsaHI	GRCGYC	1 cut(s)	102
BsaJI	CCNNGG	3 cut(s)	51, 97, 158
BsaXI	ACNNNNNCTCC	2 cut(s)	307, 337
Bsc4I	CCNNNNNNNGG	3 cut(s)	46, 98, 589
Bse118I	RCCGGY	1 cut(s)	71
Bse1I	ACTGG	1 cut(s)	583
BseDI	CCNNGG	3 cut(s)	51, 97, 158
BseGI	GGATG	3 cut(s)	37, 258, 337
BseLI	CCNNNNNNNGG	3 cut(s)	46, 98, 589
BseMII	CTCAG	2 cut(s)	57, 117
BseNI	ACTGG	1 cut(s)	583
BseRI	GAGGAG	1 cut(s)	138
BseSI	GKGCMC	1 cut(s)	92
BseX3I	CGGCCG	1 cut(s)	48
BseXI	GCAGC	3 cut(s)	87, 570, 625
Bsh1236I	CGCG	1 cut(s)	635
Bsh1285I	CGRYCG	1 cut(s)	51
BshFI	GGCC	3 cut(s)	50, 90, 164
BshNI	GGYRCC	2 cut(s)	65, 101
BsiEI	CGRYCG	1 cut(s)	51
BsiSI	CCGG	2 cut(s)	72, 590
BslFI	GGGAC	2 cut(s)	182, 527
BslI	CCNNNNNNNGG	3 cut(s)	46, 98, 589
BsmFI	GGGAC	2 cut(s)	182, 527
BsmI	GAATGC	1 cut(s)	688
BsnI	GGCC	3 cut(s)	50, 90, 164
Bsp120I	GGGCCC	1 cut(s)	88
Bsp1286I	GDGCHC	2 cut(s)	92, 645
Bsp143I	GATC	3 cut(s)	347, 386, 620
Bsp68I	TCGCGA	1 cut(s)	635
BspACI	CCGC	5 cut(s)	47, 63, 69, 123, 710
BspANI	GGCC	3 cut(s)	50, 90, 164
BspCNI	CTCAG	2 cut(s)	56, 118
BspFNI	CGCG	1 cut(s)	635
BspLI	GGNNCC	6 cut(s)	67, 90, 103, 121, 642, 654
BspMAI	CTGCAG	1 cut(s)	615
BspMI	ACCTGC	1 cut(s)	618
BspPI	GGATC	2 cut(s)	355, 628
BspT107I	GGYRCC	2 cut(s)	65, 101
BsrFI	RCCGGY	1 cut(s)	71
BsrI	ACTGG	1 cut(s)	583
BssAI	RCCGGY	1 cut(s)	71
BssECI	CCNNGG	3 cut(s)	51, 97, 158
BssMI	GATC	3 cut(s)	347, 386, 620
BssNI	GRCGYC	1 cut(s)	102
BssSI	CACGAG	1 cut(s)	247
BssT1I	CCWWGG	1 cut(s)	158
Bst2BI	CACGAG	1 cut(s)	247
Bst4CI	ACNGT	1 cut(s)	832
Bst6I	CTCTTC	1 cut(s)	764
BstACI	GRCGYC	1 cut(s)	102
BstC8I	GCNNGC	3 cut(s)	73, 80, 505
BstDEI	CTNAG	2 cut(s)	43, 126
BstDSI	CCRYGG	1 cut(s)	97
BstF5I	GGATG	3 cut(s)	37, 258, 337
BstFNI	CGCG	1 cut(s)	635
BstH2I	RGCGCY	1 cut(s)	105
BstHHI	GCGC	1 cut(s)	104
BstKTI	GATC	3 cut(s)	350, 389, 623
BstMBI	GATC	3 cut(s)	347, 386, 620
BstMCI	CGRYCG	1 cut(s)	51
BstMWI	GCNNNNNNNGC	1 cut(s)	101
BstNSI	RCATGY	3 cut(s)	280, 737, 760
BstSFI	CTRYAG	1 cut(s)	611
BstSLI	GKGCMC	1 cut(s)	92
BstUI	CGCG	1 cut(s)	635
BstV1I	GCAGC	3 cut(s)	87, 570, 625
BstV2I	GAAGAC	3 cut(s)	279, 371, 555
BstX2I	RGATCY	1 cut(s)	620
BstYI	RGATCY	1 cut(s)	620
BstZI	CGGCCG	1 cut(s)	48
BsuRI	GGCC	3 cut(s)	50, 90, 164
BtgI	CCRYGG	1 cut(s)	97
BtsCI	GGATG	3 cut(s)	37, 258, 337
BtsI	GCAGTG	2 cut(s)	467, 674
BtsIMutI	CAGTG	3 cut(s)	467, 674, 837
BtuMI	TCGCGA	1 cut(s)	635
BveI	ACCTGC	1 cut(s)	618
Cac8I	GCNNGC	3 cut(s)	73, 80, 505
CfoI	GCGC	1 cut(s)	104
Cfr10I	RCCGGY	1 cut(s)	71
Cfr13I	GGNCC	5 cut(s)	88, 89, 120, 162, 776
Csp6I	GTAC	3 cut(s)	416, 740, 798
CviAII	CATG	6 cut(s)	277, 312, 351, 700, 734, 757
CviQI	GTAC	3 cut(s)	416, 740, 798
DdeI	CTNAG	2 cut(s)	43, 126
DinI	GGCGCC	1 cut(s)	103
DpnI	GATC	3 cut(s)	349, 388, 622
DpnII	GATC	3 cut(s)	347, 386, 620
EaeI	YGGCCR	1 cut(s)	48
EagI	CGGCCG	1 cut(s)	48
Eam1104I	CTCTTC	1 cut(s)	764
EarI	CTCTTC	1 cut(s)	764
EclXI	CGGCCG	1 cut(s)	48
Eco130I	CCWWGG	1 cut(s)	158
Eco24I	GRGCYC	2 cut(s)	92, 645
Eco47I	GGWCC	2 cut(s)	120, 776
Eco52I	CGGCCG	1 cut(s)	48
Eco57I	CTGAAG	1 cut(s)	645
EcoO109I	RGGNCCY	2 cut(s)	88, 89
EcoRI	GAATTC	1 cut(s)	294
EcoT14I	CCWWGG	1 cut(s)	158
EcoT38I	GRGCYC	2 cut(s)	92, 645
EgeI	GGCGCC	1 cut(s)	103
EheI	GGCGCC	1 cut(s)	103
ErhI	CCWWGG	1 cut(s)	158
FaeI	CATG	6 cut(s)	280, 315, 354, 703, 737, 760
FalI	AAGNNNNNCTT	2 cut(s)	361, 393
FaqI	GGGAC	2 cut(s)	182, 527
FatI	CATG	6 cut(s)	276, 311, 350, 699, 733, 756
Fnu4HI	GCNGC	6 cut(s)	48, 69, 76, 559, 614, 710
FokI	GGATG	3 cut(s)	44, 265, 344
FriOI	GRGCYC	2 cut(s)	92, 645
Fsp4HI	GCNGC	6 cut(s)	48, 69, 76, 559, 614, 710
FspBI	CTAG	1 cut(s)	159
GlaI	GCGC	1 cut(s)	103
GluI	GCNGC	6 cut(s)	48, 69, 76, 559, 614, 710
HaeII	RGCGCY	1 cut(s)	105
HaeIII	GGCC	3 cut(s)	50, 90, 164
HapII	CCGG	2 cut(s)	72, 590
HhaI	GCGC	1 cut(s)	104
Hin1I	GRCGYC	1 cut(s)	102
Hin1II	CATG	6 cut(s)	280, 315, 354, 703, 737, 760
Hin6I	GCGC	1 cut(s)	102
HinP1I	GCGC	1 cut(s)	102
HinfI	GANTC	1 cut(s)	593
HpaII	CCGG	2 cut(s)	72, 590
Hpy166II	GTNNAC	3 cut(s)	186, 433, 776
Hpy188I	TCNGA	1 cut(s)	625
Hpy188III	TCNNGA	3 cut(s)	476, 634, 723
Hpy8I	GTNNAC	3 cut(s)	186, 433, 776
Hpy99I	CGWCG	1 cut(s)	265
HpyAV	CCTTC	5 cut(s)	322, 388, 406, 631, 699
HpyCH4III	ACNGT	1 cut(s)	832
HpyCH4IV	ACGT	2 cut(s)	260, 540
HpyCH4V	TGCA	8 cut(s)	82, 256, 382, 532, 613, 686, 760, 853
HpyF10VI	GCNNNNNNNGC	1 cut(s)	101
HpyF3I	CTNAG	2 cut(s)	43, 126
HpySE526I	ACGT	2 cut(s)	260, 540
Hsp92I	GRCGYC	1 cut(s)	102
Hsp92II	CATG	6 cut(s)	280, 315, 354, 703, 737, 760
HspAI	GCGC	1 cut(s)	102
KasI	GGCGCC	1 cut(s)	101
KroI	GCCGGC	1 cut(s)	71
KroNI	GCCGGC	1 cut(s)	73
Kzo9I	GATC	3 cut(s)	347, 386, 620
LmnI	GCTCC	2 cut(s)	315, 640
Lsp1109I	GCAGC	3 cut(s)	87, 570, 625
LweI	GCATC	3 cut(s)	243, 490, 696
MaeI	CTAG	1 cut(s)	159
MaeII	ACGT	2 cut(s)	260, 540
MaeIII	GTNAC	1 cut(s)	826
MalI	GATC	3 cut(s)	349, 388, 622
MboI	GATC	3 cut(s)	347, 386, 620
MboII	GAAGA	7 cut(s)	35, 68, 284, 376, 560, 638, 781
MflI	RGATCY	1 cut(s)	620
MhlI	GDGCHC	2 cut(s)	92, 645
MluCI	AATT	4 cut(s)	234, 294, 358, 508
Mly113I	GGCGCC	1 cut(s)	102
MmeI	TCCRAC	1 cut(s)	323
MroNI	GCCGGC	1 cut(s)	71
MroXI	GAANNNNTTC	1 cut(s)	398
MslI	CAYNNNNRTG	2 cut(s)	407, 704
MspA1I	CMGCKG	2 cut(s)	47, 125
MspI	CCGG	2 cut(s)	72, 590
Mva1269I	GAATGC	1 cut(s)	688
MvnI	CGCG	1 cut(s)	635
MwoI	GCNNNNNNNGC	1 cut(s)	101
NaeI	GCCGGC	1 cut(s)	73
NarI	GGCGCC	1 cut(s)	102
NdeII	GATC	3 cut(s)	347, 386, 620
NgoMIV	GCCGGC	1 cut(s)	71
NlaIII	CATG	6 cut(s)	280, 315, 354, 703, 737, 760
NlaIV	GGNNCC	6 cut(s)	67, 90, 103, 121, 642, 654
NmeAIII	GCCGAG	1 cut(s)	76
NruI	TCGCGA	1 cut(s)	635
NspI	RCATGY	3 cut(s)	280, 737, 760
PciI	ACATGT	2 cut(s)	276, 733
PctI	GAATGC	1 cut(s)	688
PdiI	GCCGGC	1 cut(s)	73
PdmI	GAANNNNTTC	1 cut(s)	398
PfeI	GAWTC	1 cut(s)	593
PkrI	GCNGC	6 cut(s)	49, 70, 77, 560, 615, 711
PluTI	GGCGCC	1 cut(s)	105
PscI	ACATGT	2 cut(s)	276, 733
PsiI	TTATAA	1 cut(s)	603
PspN4I	GGNNCC	6 cut(s)	67, 90, 103, 121, 642, 654
PspOMI	GGGCCC	1 cut(s)	88
PspPI	GGNCC	5 cut(s)	88, 89, 120, 162, 776
PstI	CTGCAG	1 cut(s)	615
PsuI	RGATCY	1 cut(s)	620
RruI	TCGCGA	1 cut(s)	635
RsaI	GTAC	3 cut(s)	417, 741, 799
RsaNI	GTAC	3 cut(s)	416, 740, 798
RseI	CAYNNNNRTG	2 cut(s)	407, 704
SatI	GCNGC	6 cut(s)	48, 69, 76, 559, 614, 710
Sau3AI	GATC	3 cut(s)	347, 386, 620
Sau96I	GGNCC	5 cut(s)	88, 89, 120, 162, 776
SduI	GDGCHC	2 cut(s)	92, 645
SfaNI	GCATC	3 cut(s)	243, 490, 696
SfcI	CTRYAG	1 cut(s)	611
SfoI	GGCGCC	1 cut(s)	103
SinI	GGWCC	2 cut(s)	120, 776
SmiMI	CAYNNNNRTG	2 cut(s)	407, 704
Sse9I	AATT	4 cut(s)	234, 294, 358, 508
SsiI	CCGC	5 cut(s)	47, 63, 69, 123, 710
SspDI	GGCGCC	1 cut(s)	101
SspMI	CTAG	1 cut(s)	159
StyI	CCWWGG	1 cut(s)	158
TaaI	ACNGT	1 cut(s)	832
TaiI	ACGT	2 cut(s)	263, 543
TasI	AATT	4 cut(s)	234, 294, 358, 508
TauI	GCSGC	3 cut(s)	50, 71, 712
TfiI	GAWTC	1 cut(s)	593
TscAI	CASTG	3 cut(s)	467, 681, 837
TseI	GCWGC	3 cut(s)	75, 558, 613
TspDTI	ATGAA	2 cut(s)	425, 681
TspRI	CASTG	3 cut(s)	467, 681, 837
VpaK11BI	GGWCC	2 cut(s)	120, 776
XapI	RAATTY	2 cut(s)	234, 294
XceI	RCATGY	3 cut(s)	280, 737, 760
XcmI	CCANNNNNNNNNTGG	2 cut(s)	246, 500
XmaJI	CCTAGG	1 cut(s)	158
XmnI	GAANNNNTTC	1 cut(s)	398
XspI	CTAG	1 cut(s)	159

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.