Rorug05G0258700

SNF1-related protein kinase regulatory

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003241
Physical Location & Seq
Forward (+)
29067517 .. 29072082
4566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0258700.1

Sequence Viewer

Length: 1311 bp
ATGGAGAACTTCCGACAACCTTTCTTTCCTTCCACAACAACCACCATGACCTCCATTTATGAACCTATGAGTTCCTCCCCTTCAACTCTTGCTAGTTCATCCTCTCTGTCCTATCATTATCCAGCAGTTCAGTACCTACCACCACTCAATGGCGGCAGCCAGCTGTACTTTCAAAGCTCATACCCTAATTACCAGAAGCAGGCCTCTCAGCCCCCCTTCCAATATCCCAGGTATCCAGTCCTGCTTCCTTGGCCACAGCCAAATCCAGTCTCTGGAGATCAAGAAGGGATTGAGTCCAAGGCTGCAAGAGATGAGCAAAGGATCGCTCGCCAAAGAAGACTCTTTTTAAGCAGAAGCGCTGCTGACTCTGGGTCCTCATCAAGTTCAATGCAGATGGATTTATGTGGTGCTGATTCTGATATGCAGAACACAGACCTCTGCACGCCGGATATGAAGGAATTGAGAGTGTTGTTCAAGAAGCAGTTGAAGAAGAGTGATGTTGGATCCTGGGGCAGGATTGTCGTTCCAAAGAAGGAGGCAGAGAAATACCTTCCAAGCCTTGCTGAAAAACAAGGCATCGTGATGAGGATCAGAGATGTACATTCCGGTCACGTGTGGGGCATGAAATACAAGTACTGGGCAAATAACAAAAGTCGAATGTATGTCTTTGAAAGTGCAGGAAGCTTTATTAGGAAAAATGGACTGGAGGTGGGAGATTGCATTTCTCTTTACAAGGATGAGAGCAAGAACTTTTACTTCTCCATAGAAAAGGCAGGAACAAGACCATCACATGTAACTGAGTCCTCGTATGAACAACAGCACTGTGCTACCAACTGTAAAGACGGTAACGATGATGACAACAGCAGTAGCATCAACACACAGATTACCAACTCTGACGATAACAATCACAAGAACAACACCAACACCTACATGAACTCCAGCAACAATGCTGACATCCACAGCGAAACCGATTCCAACAATACAAAATTGTACACACCATATACCTTCAATGAAGAAGATGAATGGTATCTAGCAGCACTGTTGGAAGATGAATCAAACAAGGTGGTGACACTGTCTGCGGATCCTGCTTTTTCATGTGGACGACTTAAGGAAGTCAATAGCTCCATCGACAATGTCATCAGTACTCAATTAGAAAGCTCAACTCAACCAGCTATGGCAGGAATTGGGGCCTCATCATCGTCCTCACAGAGCACAGTGAAGATGGTGGAGGATCACTTCGATGACTGTTACAAAGGGCTTGGGACGCTTCCGGCAGTTGATCGTTATGAGCATGATGATTTTTTATTATAA

Protein Analysis

436

Amino Acids

48.9

Weight (kDa)

5.23

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 158 - 256 1.3e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1309
AccB7I CCANNNNNTGG 2 cut(s) 149, 272
AciI CCGC 2 cut(s) 153, 1079
AclWI GGATC 7 cut(s) 329, 498, 511, 596, 1076, 1089, 1239
AcoI YGGCCR 1 cut(s) 251
AcvI CACGTG 1 cut(s) 613
AfaI GTAC 6 cut(s) 134, 167, 600, 635, 992, 1144
AfeI AGCGCT 1 cut(s) 358
AfiI CCNNNNNNNGG 4 cut(s) 149, 199, 272, 513
AflII CTTAAG 1 cut(s) 1106
AflIII ACRYGT 2 cut(s) 612, 790
AgsI TTSAA 7 cut(s) 84, 173, 387, 475, 487, 671, 1009
AhdI GACNNNNNGTC 1 cut(s) 370
AjnI CCWGG 2 cut(s) 227, 506
AluBI AGCT 6 cut(s) 163, 177, 684, 1122, 1158, 1172
AluI AGCT 6 cut(s) 163, 177, 684, 1122, 1158, 1172
Alw21I GWGCWC 1 cut(s) 1214
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 7 cut(s) 329, 498, 511, 596, 1076, 1089, 1239
AlwNI CAGNNNCTG 1 cut(s) 272
Aor51HI AGCGCT 1 cut(s) 358
AoxI GGCC 3 cut(s) 201, 251, 1188
ApeKI GCWGC 4 cut(s) 156, 302, 359, 1034
AspLEI GCGC 1 cut(s) 359
AspS9I GGNCC 2 cut(s) 372, 1188
AsuHPI GGTGA 1 cut(s) 1078
AvaII GGWCC 1 cut(s) 372
BalI TGGCCA 1 cut(s) 253
BamHI GGATCC 2 cut(s) 503, 1081
BbrPI CACGTG 1 cut(s) 613
BbsI GAAGAC 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 1214
BbvI GCAGC 4 cut(s) 168, 289, 346, 1046
BccI CCATC 4 cut(s) 388, 793, 1133, 1216
BcgI CGANNNNNNTGC 2 cut(s) 502, 536
BciT130I CCWGG 2 cut(s) 229, 508
BciVI GTATCC 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 2 cut(s) 93, 1031
BfoI RGCGCY 1 cut(s) 360
BfrI CTTAAG 1 cut(s) 1106
BfuI GTATCC 1 cut(s) 243
BisI GCNGC 5 cut(s) 154, 157, 303, 360, 1035
BlsI GCNGC 5 cut(s) 155, 158, 304, 361, 1036
BmcAI AGTACT 2 cut(s) 635, 1144
Bme1390I CCNGG 2 cut(s) 229, 508
Bme18I GGWCC 1 cut(s) 372
BmeRI GACNNNNNGTC 1 cut(s) 370
BmgT120I GGNCC 2 cut(s) 372, 1188
BmiI GGNNCC 4 cut(s) 373, 505, 1083, 1189
BmrFI CCNGG 2 cut(s) 229, 508
BmrI ACTGGG 1 cut(s) 646
BmsI GCATC 2 cut(s) 585, 879
BmuI ACTGGG 1 cut(s) 646
BpiI GAAGAC 1 cut(s) 343
BpmI CTGGAG 3 cut(s) 294, 725, 922
BsaAI YACGTR 1 cut(s) 613
BsaBI GATNNNNATC 2 cut(s) 587, 903
BsaJI CCNNGG 4 cut(s) 227, 248, 297, 507
BsaWI WCCGGW 1 cut(s) 605
BsaXI ACNNNNNCTCC 2 cut(s) 920, 950
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 199, 272, 513
Bse1I ACTGG 4 cut(s) 236, 266, 641, 708
Bse8I GATNNNNATC 2 cut(s) 587, 903
BseBI CCWGG 2 cut(s) 229, 508
BseDI CCNNGG 4 cut(s) 227, 248, 297, 507
BseGI GGATG 3 cut(s) 98, 742, 954
BseJI GATNNNNATC 2 cut(s) 587, 903
BseLI CCNNNNNNNGG 4 cut(s) 149, 199, 272, 513
BseMII CTCAG 2 cut(s) 221, 789
BseNI ACTGG 4 cut(s) 236, 266, 641, 708
BseXI GCAGC 4 cut(s) 168, 289, 346, 1046
BsgI GTGCAG 2 cut(s) 424, 696
BshFI GGCC 3 cut(s) 203, 253, 1190
BsiHKAI GWGCWC 1 cut(s) 1214
BsiSI CCGG 3 cut(s) 446, 606, 1271
BslFI GGGAC 1 cut(s) 1276
BslI CCNNNNNNNGG 4 cut(s) 149, 199, 272, 513
BsmAI GTCTC 1 cut(s) 274
BsmFI GGGAC 1 cut(s) 1276
BsnI GGCC 3 cut(s) 203, 253, 1190
Bsp1286I GDGCHC 1 cut(s) 1214
Bsp1407I TGTACA 2 cut(s) 598, 990
Bsp143I GATC 7 cut(s) 277, 321, 503, 588, 1081, 1231, 1279
BspACI CCGC 2 cut(s) 153, 1079
BspANI GGCC 3 cut(s) 203, 253, 1190
BspCNI CTCAG 2 cut(s) 220, 790
BspLI GGNNCC 4 cut(s) 373, 505, 1083, 1189
BspPI GGATC 7 cut(s) 329, 498, 511, 596, 1076, 1089, 1239
BspTI CTTAAG 1 cut(s) 1106
BsrGI TGTACA 2 cut(s) 598, 990
BsrI ACTGG 4 cut(s) 236, 266, 641, 708
BssECI CCNNGG 4 cut(s) 227, 248, 297, 507
BssMI GATC 7 cut(s) 277, 321, 503, 588, 1081, 1231, 1279
BssT1I CCWWGG 2 cut(s) 248, 297
Bst2UI CCWGG 2 cut(s) 229, 508
Bst4CI ACNGT 7 cut(s) 824, 836, 845, 1041, 1074, 1216, 1247
Bst6I CTCTTC 1 cut(s) 485
BstAFI CTTAAG 1 cut(s) 1106
BstAUI TGTACA 2 cut(s) 598, 990
BstBAI YACGTR 1 cut(s) 613
BstC8I GCNNGC 4 cut(s) 161, 201, 328, 443
BstDEI CTNAG 2 cut(s) 207, 798
BstENI CCTNNNNNAGG 1 cut(s) 511
BstF5I GGATG 3 cut(s) 98, 742, 954
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 7 cut(s) 280, 324, 506, 591, 1084, 1234, 1282
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 7 cut(s) 277, 321, 503, 588, 1081, 1231, 1279
BstMWI GCNNNNNNNGC 3 cut(s) 250, 1085, 1264
BstNI CCWGG 2 cut(s) 229, 508
BstNSI RCATGY 1 cut(s) 794
BstSCI CCNGG 2 cut(s) 227, 506
BstV1I GCAGC 4 cut(s) 168, 289, 346, 1046
BstV2I GAAGAC 1 cut(s) 343
BstX2I RGATCY 2 cut(s) 503, 1081
BstYI RGATCY 2 cut(s) 503, 1081
BsuI GTATCC 1 cut(s) 243
BsuRI GGCC 3 cut(s) 203, 253, 1190
BtsCI GGATG 3 cut(s) 98, 742, 954
BtsIMutI CAGTG 4 cut(s) 820, 1037, 1070, 1221
Cac8I GCNNGC 4 cut(s) 161, 201, 328, 443
CaiI CAGNNNCTG 1 cut(s) 272
CfoI GCGC 1 cut(s) 359
Cfr13I GGNCC 2 cut(s) 372, 1188
CseI GACGC 1 cut(s) 1273
Csp6I GTAC 6 cut(s) 133, 166, 599, 634, 991, 1143
CspCI CAANNNNNGTGG 2 cut(s) 1044, 1079
CviAII CATG 6 cut(s) 46, 622, 791, 931, 1095, 1292
CviQI GTAC 6 cut(s) 133, 166, 599, 634, 991, 1143
DdeI CTNAG 2 cut(s) 207, 798
DpnI GATC 7 cut(s) 279, 323, 505, 590, 1083, 1233, 1281
DpnII GATC 7 cut(s) 277, 321, 503, 588, 1081, 1231, 1279
DriI GACNNNNNGTC 1 cut(s) 370
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 485
Eam1105I GACNNNNNGTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 485
Eco130I CCWWGG 2 cut(s) 248, 297
Eco147I AGGCCT 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 372
Eco47III AGCGCT 1 cut(s) 358
Eco72I CACGTG 1 cut(s) 613
EcoNI CCTNNNNNAGG 1 cut(s) 511
EcoO109I RGGNCCY 2 cut(s) 372, 1188
EcoRII CCWGG 2 cut(s) 227, 506
EcoT14I CCWWGG 2 cut(s) 248, 297
ErhI CCWWGG 2 cut(s) 248, 297
FaeI CATG 6 cut(s) 49, 625, 794, 934, 1098, 1295
FaqI GGGAC 1 cut(s) 1276
FatI CATG 6 cut(s) 45, 621, 790, 930, 1094, 1291
Fnu4HI GCNGC 5 cut(s) 154, 157, 303, 360, 1035
FokI GGATG 3 cut(s) 85, 749, 941
Fsp4HI GCNGC 5 cut(s) 154, 157, 303, 360, 1035
FspBI CTAG 2 cut(s) 93, 1031
GlaI GCGC 1 cut(s) 358
GluI GCNGC 5 cut(s) 154, 157, 303, 360, 1035
GsuI CTGGAG 3 cut(s) 294, 725, 922
HaeII RGCGCY 1 cut(s) 360
HaeIII GGCC 3 cut(s) 203, 253, 1190
HapII CCGG 3 cut(s) 446, 606, 1271
HgaI GACGC 1 cut(s) 1273
HhaI GCGC 1 cut(s) 359
Hin1II CATG 6 cut(s) 49, 625, 794, 934, 1098, 1295
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 682
HinfI GANTC 7 cut(s) 293, 339, 365, 413, 800, 971, 1052
HpaII CCGG 3 cut(s) 446, 606, 1271
HphI GGTGA 1 cut(s) 1078
Hpy166II GTNNAC 2 cut(s) 993, 1100
Hpy188I TCNGA 4 cut(s) 14, 418, 593, 895
Hpy188III TCNNGA 4 cut(s) 273, 281, 475, 580
Hpy8I GTNNAC 2 cut(s) 993, 1100
HpyAV CCTTC 8 cut(s) 39, 90, 226, 278, 448, 526, 560, 1015
HpyCH4III ACNGT 7 cut(s) 824, 836, 845, 1041, 1074, 1216, 1247
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 6 cut(s) 305, 391, 424, 441, 677, 720
HpyF10VI GCNNNNNNNGC 3 cut(s) 250, 1085, 1264
HpyF3I CTNAG 2 cut(s) 207, 798
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 6 cut(s) 49, 625, 794, 934, 1098, 1295
HspAI GCGC 1 cut(s) 357
Kzo9I GATC 7 cut(s) 277, 321, 503, 588, 1081, 1231, 1279
LmnI GCTCC 1 cut(s) 1127
Lsp1109I GCAGC 4 cut(s) 168, 289, 346, 1046
LweI GCATC 2 cut(s) 585, 879
MaeI CTAG 2 cut(s) 93, 1031
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 5 cut(s) 608, 793, 845, 1066, 1247
MalI GATC 7 cut(s) 279, 323, 505, 590, 1083, 1233, 1281
MboI GATC 7 cut(s) 277, 321, 503, 588, 1081, 1231, 1279
MboII GAAGA 7 cut(s) 348, 499, 502, 1025, 1028, 1058, 1231
MflI RGATCY 2 cut(s) 503, 1081
MhlI GDGCHC 1 cut(s) 1214
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 5 cut(s) 187, 458, 986, 1148, 1182
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 4 cut(s) 302, 333, 359, 809
MmeI TCCRAC 4 cut(s) 37, 481, 999, 1023
Mox20I TGGCCA 1 cut(s) 253
MscI TGGCCA 1 cut(s) 253
MseI TTAA 2 cut(s) 347, 1107
MslI CAYNNNNRTG 3 cut(s) 581, 929, 1239
Msp20I TGGCCA 1 cut(s) 253
MspA1I CMGCKG 1 cut(s) 163
MspCI CTTAAG 1 cut(s) 1106
MspI CCGG 3 cut(s) 446, 606, 1271
MspR9I CCNGG 2 cut(s) 229, 508
MvaI CCWGG 2 cut(s) 229, 508
MwoI GCNNNNNNNGC 3 cut(s) 250, 1085, 1264
NdeII GATC 7 cut(s) 277, 321, 503, 588, 1081, 1231, 1279
NlaIII CATG 6 cut(s) 49, 625, 794, 934, 1098, 1295
NlaIV GGNNCC 4 cut(s) 373, 505, 1083, 1189
NmuCI GTSAC 2 cut(s) 608, 1066
NspI RCATGY 1 cut(s) 794
PceI AGGCCT 1 cut(s) 203
PciI ACATGT 1 cut(s) 790
PfeI GAWTC 3 cut(s) 413, 971, 1052
PflFI GACNNNGTC 2 cut(s) 1072, 1133
PflMI CCANNNNNTGG 2 cut(s) 149, 272
PkrI GCNGC 5 cut(s) 155, 158, 304, 361, 1036
PleI GAGTC 4 cut(s) 301, 333, 359, 808
PmaCI CACGTG 1 cut(s) 613
PmlI CACGTG 1 cut(s) 613
PpsI GAGTC 4 cut(s) 301, 333, 359, 808
Ppu21I YACGTR 1 cut(s) 613
PpuMI RGGWCCY 1 cut(s) 372
PscI ACATGT 1 cut(s) 790
PsiI TTATAA 1 cut(s) 1309
Psp5II RGGWCCY 1 cut(s) 372
Psp6I CCWGG 2 cut(s) 227, 506
PspCI CACGTG 1 cut(s) 613
PspGI CCWGG 2 cut(s) 227, 506
PspN4I GGNNCC 4 cut(s) 373, 505, 1083, 1189
PspPI GGNCC 2 cut(s) 372, 1188
PspPPI RGGWCCY 1 cut(s) 372
PstNI CAGNNNCTG 1 cut(s) 272
PsuI RGATCY 2 cut(s) 503, 1081
PsyI GACNNNGTC 2 cut(s) 1072, 1133
PvuII CAGCTG 1 cut(s) 163
RsaI GTAC 6 cut(s) 134, 167, 600, 635, 992, 1144
RsaNI GTAC 6 cut(s) 133, 166, 599, 634, 991, 1143
RseI CAYNNNNRTG 3 cut(s) 581, 929, 1239
SaqAI TTAA 2 cut(s) 347, 1107
SatI GCNGC 5 cut(s) 154, 157, 303, 360, 1035
Sau3AI GATC 7 cut(s) 277, 321, 503, 588, 1081, 1231, 1279
Sau96I GGNCC 2 cut(s) 372, 1188
ScaI AGTACT 2 cut(s) 635, 1144
SchI GAGTC 4 cut(s) 302, 333, 359, 809
ScrFI CCNGG 2 cut(s) 229, 508
SduI GDGCHC 1 cut(s) 1214
SfaNI GCATC 2 cut(s) 585, 879
SinI GGWCC 1 cut(s) 372
SmiMI CAYNNNNRTG 3 cut(s) 581, 929, 1239
SmlI CTYRAG 1 cut(s) 1106
SmoI CTYRAG 1 cut(s) 1106
Sse9I AATT 5 cut(s) 187, 458, 986, 1148, 1182
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 2 cut(s) 153, 1079
SspMI CTAG 2 cut(s) 93, 1031
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 2 cut(s) 227, 506
StyI CCWWGG 2 cut(s) 248, 297
TaaI ACNGT 7 cut(s) 824, 836, 845, 1041, 1074, 1216, 1247
TaiI ACGT 1 cut(s) 615
TaqI TCGA 3 cut(s) 655, 1128, 1239
TasI AATT 5 cut(s) 187, 458, 986, 1148, 1182
TatI WGTACW 5 cut(s) 165, 598, 633, 990, 1142
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 3 cut(s) 413, 971, 1052
Tru1I TTAA 2 cut(s) 347, 1107
Tru9I TTAA 2 cut(s) 347, 1107
TscAI CASTG 4 cut(s) 827, 1044, 1077, 1221
TseFI GTSAC 2 cut(s) 608, 1066
TseI GCWGC 4 cut(s) 156, 302, 359, 1034
Tsp45I GTSAC 2 cut(s) 608, 1066
TspRI CASTG 4 cut(s) 827, 1044, 1077, 1221
Tth111I GACNNNGTC 2 cut(s) 1072, 1133
Van91I CCANNNNNTGG 2 cut(s) 149, 272
Vha464I CTTAAG 1 cut(s) 1106
VpaK11BI GGWCC 1 cut(s) 372
XagI CCTNNNNNAGG 1 cut(s) 511
XceI RCATGY 1 cut(s) 794
XspI CTAG 2 cut(s) 93, 1031
ZrmI AGTACT 2 cut(s) 635, 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.