Rorug04G0385200

SNF1-related protein kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003241
Physical Location & Seq
Reverse (-)
55529461 .. 55533353
3893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0385200.1

Sequence Viewer

Length: 1662 bp
ATGGAAACCCACAGCTCCTGTAGCATCGCCGCCACAAACGACGACGGCGAATGGAGAGCGGAAAAAGCTATAGGCGGCAACGCCCAAGCTCTCCAAGCTCTCAGAGAACTCATCGTCTACCCTCTCCTCTTTTCTCGCCAAGCCAAAAAGCTCGGTCTCAAATGGCCCCGAGGTTTGCTTCTCTACGGTCCTCCCGGCACCGGAAAGACAAGCTTGGTCCGCGCAGTGGTTCAGGAATGTGGTGCGCATTTAGTTCTCATCAGTCCGCATACTGTTCACAGAGCTTATACCGGAGAGAGTGAGTTAGCACTGCGAGAGGCTTTTGCGGAGGCCGCGTCACATACATTGTCAGGGAAGCCGTCTGTTGTGTTTATAGATGAAATTGATGCCCTCTGTCCTCGCCGTGATTCGAGAAGAGAGCAAGATGTTCGTATAGCTGCTCAACTGCGTTTGCTGATGGAGTCAAAGGCTTCGTCGACGGATGTACCACAAGTTGTTGTTGTTGCCTCCACTAATAGAGTGGAAGCAGTAGACCCAGCACTGAGAAGGTCTGGCCGTTTTGATGCTGAAATTGAAGTTACTACACCTACTGAAGATGAACGTTTTCAAATCCTCAAGCTCTACACAAGAAAGATTTCTTTGGATCCTGATGTTGACCTACGAGCCTTGGCTGCAAAATGCAATGGATTTGCTGGAGCTGACCTGGAAGCTTTATGTCGTGAGGCTGCCATGTCTGCAATGAGGAGGTATCAAGATGCAGACAAAGATGTTGGTTTGCTAAGCTTAGCAAAAGAAGACTGGGAGAATGCAAAGTCTGTAGTTAATGCAAGTATAACAAGAGGTGTCACGGTGGAAATCCCCAAGGTGACATGGGAAGATATTGGAGGCTTGAAAGATTTAAAGATAAAGCTCCAGCAAGCTGTTGAGTGGCCTATTAAATATTCTACAGAGTTTTCAAGGCTGGGAATATCTCCTATACGCGGAATTCTTCTTTATGGTCCACCAGGATGTTCAAAAACCACCCTTGCCAAAGCCGCTGCAAATGCTGCCCAAGCTTCTTTTTTTTCTTTGAGTGGTGCGGAATTGTTTTCAATGTATGTTGGAGAGGGTGAAGCTCTGCTCCGCAATACCTTTCGGAGAGCTCGCCTTGCAGCACCCAGCATTATATTCTTTGATGAGGCAGATGTTGTTGCAGCCAAACGGGGTGGGAGCTCAAGCAACAGCAGTACTGTTGGAGAGAGGCTTCTATCTACTTTGCTTACTGAAATGGATGGTTTGGAAGAGGCTAAGGGAGTTTTTGTCTTGGCTGCTACAAATCGTCCTCACGCAATTGACGCTGCATTAGTTCGACCAGGACGCTTTGATTTGGTGCTCTTTGTACAACCACCGGATTTAGAAGGTCGAAAGCAGATCCTTAGTGTACTCACTCGTGAGATGAAAATAGGTGATGATGTCGATCTCAAACGAATAGCAGAAGAAACAGAGCACTTCACTGGAGCTGAGCTGAAGGGTCTATGCAAGGAAGCCGGAATAGTAGCTCTGAGAGAAGACATATCTGCCAGTGTTGTGTGTCATCGCCATTTCCAGACTGCAAAGGACTCTTTAAAGCCAGCATTGAGCAAAGCAGATGTTGACTCATACAAGGCTTTTGCCGCTGCGTAG

Protein Analysis

553

Amino Acids

60.1

Weight (kDa)

6.38

Isoelectric Point (pI)

32.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 56 - 167 2.7e-06 AAA ATPase domain
AAA PF00004 59 - 194 3.7e-35 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 217 - 254 1.4e-14 AAA+ lid domain
AAA PF00004 329 - 460 5e-39 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 484 - 529 2.8e-15 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 197
AccBSI CCGCTC 1 cut(s) 59
AccI GTMKAC 3 cut(s) 117, 476, 531
AccII CGCG 3 cut(s) 222, 335, 981
AclI AACGTT 1 cut(s) 601
AclWI GGATC 3 cut(s) 638, 651, 1405
AcoI YGGCCR 1 cut(s) 553
AcsI RAATTY 1 cut(s) 984
AcuI CTGAAG 2 cut(s) 612, 1526
AfaI GTAC 4 cut(s) 486, 1228, 1380, 1422
AfiI CCNNNNNNNGG 3 cut(s) 200, 226, 980
AgsI TTSAA 6 cut(s) 575, 608, 892, 957, 1014, 1092
AjnI CCWGG 3 cut(s) 702, 1003, 1351
Alw21I GWGCWC 4 cut(s) 1144, 1214, 1374, 1488
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 3 cut(s) 638, 651, 1405
AlwNI CAGNNNCTG 1 cut(s) 18
Ama87I CYCGRG 1 cut(s) 168
AoxI GGCC 4 cut(s) 164, 330, 553, 929
ApoI RAATTY 1 cut(s) 984
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
Asp700I GAANNNNTTC 2 cut(s) 603, 634
AspLEI GCGC 2 cut(s) 224, 247
AspS9I GGNCC 4 cut(s) 165, 188, 217, 998
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 3 cut(s) 877, 1121, 1457
AvaI CYCGRG 1 cut(s) 168
AvaII GGWCC 3 cut(s) 188, 217, 998
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BamHI GGATCC 1 cut(s) 643
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 2 cut(s) 1144, 1214
BauI CACGAG 1 cut(s) 1428
BbsI GAAGAC 2 cut(s) 801, 1554
Bbv12I GWGCWC 4 cut(s) 1144, 1214, 1374, 1488
BccI CCATC 2 cut(s) 451, 1265
BceAI ACGGC 4 cut(s) 61, 343, 387, 540
BcgI CGANNNNNNTGC 2 cut(s) 410, 444
BciT130I CCWGG 3 cut(s) 704, 1005, 1353
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 161
BfmI CTRYAG 4 cut(s) 19, 69, 816, 945
BlpI GCTNAGC 3 cut(s) 779, 784, 1500
BmcAI AGTACT 1 cut(s) 1228
Bme1390I CCNGG 4 cut(s) 195, 704, 1005, 1353
Bme18I GGWCC 3 cut(s) 188, 217, 998
BmeT110I CYCGRG 1 cut(s) 168
BmgT120I GGNCC 4 cut(s) 165, 188, 217, 998
BmiI GGNNCC 3 cut(s) 167, 199, 645
BmrFI CCNGG 4 cut(s) 195, 704, 1005, 1353
BmrI ACTGGG 1 cut(s) 808
BmsI GCATC 4 cut(s) 33, 376, 553, 745
BmuI ACTGGG 1 cut(s) 808
BpiI GAAGAC 2 cut(s) 801, 1554
BpmI CTGGAG 3 cut(s) 714, 896, 1515
Bpu10I CCTNAGC 1 cut(s) 1287
Bpu1102I GCTNAGC 3 cut(s) 779, 784, 1500
BpuEI CTTGAG 2 cut(s) 599, 1198
BpuMI CCSGG 1 cut(s) 195
BsaBI GATNNNNATC 1 cut(s) 1455
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 169, 666, 861
BsaWI WCCGGW 3 cut(s) 200, 290, 1387
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 1284, 1314
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 226, 980
Bse1I ACTGG 3 cut(s) 803, 1498, 1560
Bse3DI GCAATG 2 cut(s) 688, 744
Bse8I GATNNNNATC 1 cut(s) 1455
BseBI CCWGG 3 cut(s) 704, 1005, 1353
BseDI CCNNGG 3 cut(s) 169, 666, 861
BseGI GGATG 3 cut(s) 487, 1013, 1276
BseJI GATNNNNATC 1 cut(s) 1455
BseLI CCNNNNNNNGG 3 cut(s) 200, 226, 980
BseMI GCAATG 2 cut(s) 688, 744
BseMII CTCAG 4 cut(s) 115, 533, 1491, 1532
BseNI ACTGG 3 cut(s) 803, 1498, 1560
BseRI GAGGAG 2 cut(s) 116, 757
BseYI CCCAGC 3 cut(s) 535, 961, 1157
Bsh1236I CGCG 3 cut(s) 222, 335, 981
BshFI GGCC 4 cut(s) 166, 332, 555, 931
BshNI GGYRCC 1 cut(s) 197
BsiHKAI GWGCWC 4 cut(s) 1144, 1214, 1374, 1488
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 5 cut(s) 195, 201, 291, 1388, 1527
BslI CCNNNNNNNGG 3 cut(s) 200, 226, 980
BsmAI GTCTC 1 cut(s) 161
BsmI GAATGC 1 cut(s) 811
BsnI GGCC 4 cut(s) 166, 332, 555, 931
Bso31I GGTCTC 1 cut(s) 161
BsoBI CYCGRG 1 cut(s) 168
Bsp1286I GDGCHC 4 cut(s) 1144, 1214, 1374, 1488
Bsp1407I TGTACA 1 cut(s) 1378
Bsp143I GATC 3 cut(s) 643, 1410, 1456
Bsp1720I GCTNAGC 3 cut(s) 779, 784, 1500
BspANI GGCC 4 cut(s) 166, 332, 555, 931
BspCNI CTCAG 4 cut(s) 114, 534, 1492, 1533
BspFNI CGCG 3 cut(s) 222, 335, 981
BspLI GGNNCC 3 cut(s) 167, 199, 645
BspPI GGATC 3 cut(s) 638, 651, 1405
BspT107I GGYRCC 1 cut(s) 197
BspTNI GGTCTC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 59
BsrDI GCAATG 2 cut(s) 688, 744
BsrGI TGTACA 1 cut(s) 1378
BsrI ACTGG 3 cut(s) 803, 1498, 1560
BssECI CCNNGG 3 cut(s) 169, 666, 861
BssMI GATC 3 cut(s) 643, 1410, 1456
BssSI CACGAG 1 cut(s) 1428
BssT1I CCWWGG 2 cut(s) 666, 861
Bst2BI CACGAG 1 cut(s) 1428
Bst2UI CCWGG 3 cut(s) 704, 1005, 1353
Bst4CI ACNGT 4 cut(s) 188, 274, 850, 1231
Bst6I CTCTTC 2 cut(s) 409, 1275
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstAUI TGTACA 1 cut(s) 1378
BstC8I GCNNGC 3 cut(s) 918, 1144, 1611
BstDEI CTNAG 8 cut(s) 101, 542, 779, 784, 1287, 1415, 1500, 1541
BstF5I GGATG 3 cut(s) 487, 1013, 1276
BstFNI CGCG 3 cut(s) 222, 335, 981
BstHHI GCGC 2 cut(s) 224, 247
BstKTI GATC 3 cut(s) 646, 1413, 1459
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 3 cut(s) 643, 1410, 1456
BstNI CCWGG 3 cut(s) 704, 1005, 1353
BstSCI CCNGG 4 cut(s) 193, 702, 1003, 1351
BstSFI CTRYAG 4 cut(s) 19, 69, 816, 945
BstUI CGCG 3 cut(s) 222, 335, 981
BstV2I GAAGAC 2 cut(s) 801, 1554
BstX2I RGATCY 2 cut(s) 643, 1410
BstYI RGATCY 2 cut(s) 643, 1410
BsuRI GGCC 4 cut(s) 166, 332, 555, 931
BtgZI GCGATG 2 cut(s) 10, 1559
BtsCI GGATG 3 cut(s) 487, 1013, 1276
BtsI GCAGTG 2 cut(s) 231, 308
BtsIMutI CAGTG 5 cut(s) 231, 308, 539, 1491, 1567
Cac8I GCNNGC 3 cut(s) 918, 1144, 1611
CaiI CAGNNNCTG 1 cut(s) 18
CfoI GCGC 2 cut(s) 224, 247
Cfr13I GGNCC 4 cut(s) 165, 188, 217, 998
CseI GACGC 3 cut(s) 324, 1343, 1365
Csp6I GTAC 4 cut(s) 485, 1227, 1379, 1421
CspCI CAANNNNNGTGG 2 cut(s) 1186, 1221
CviAII CATG 2 cut(s) 730, 870
CviQI GTAC 4 cut(s) 485, 1227, 1379, 1421
DdeI CTNAG 8 cut(s) 101, 542, 779, 784, 1287, 1415, 1500, 1541
DpnI GATC 3 cut(s) 645, 1412, 1458
DpnII GATC 3 cut(s) 643, 1410, 1456
DraI TTTAAA 2 cut(s) 900, 1605
EaeI YGGCCR 1 cut(s) 553
Eam1104I CTCTTC 2 cut(s) 409, 1275
EarI CTCTTC 2 cut(s) 409, 1275
Ecl136II GAGCTC 2 cut(s) 1142, 1212
Eco130I CCWWGG 2 cut(s) 666, 861
Eco24I GRGCYC 2 cut(s) 1144, 1214
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 3 cut(s) 188, 217, 998
Eco53kI GAGCTC 2 cut(s) 1142, 1212
Eco57I CTGAAG 2 cut(s) 612, 1526
Eco88I CYCGRG 1 cut(s) 168
EcoICRI GAGCTC 2 cut(s) 1142, 1212
EcoRI GAATTC 1 cut(s) 984
EcoRII CCWGG 3 cut(s) 702, 1003, 1351
EcoT14I CCWWGG 2 cut(s) 666, 861
EcoT38I GRGCYC 2 cut(s) 1144, 1214
ErhI CCWWGG 2 cut(s) 666, 861
FaeI CATG 2 cut(s) 733, 873
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 2 cut(s) 729, 869
FblI GTMKAC 3 cut(s) 117, 476, 531
FokI GGATG 3 cut(s) 494, 1020, 1283
FriOI GRGCYC 2 cut(s) 1144, 1214
FspAI RTGCGCAY 1 cut(s) 246
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 2 cut(s) 223, 246
GsaI CCCAGC 3 cut(s) 539, 965, 1161
GsuI CTGGAG 3 cut(s) 714, 896, 1515
HaeIII GGCC 4 cut(s) 166, 332, 555, 931
HapII CCGG 5 cut(s) 195, 201, 291, 1388, 1527
HgaI GACGC 3 cut(s) 324, 1343, 1365
HhaI GCGC 2 cut(s) 224, 247
Hin1II CATG 2 cut(s) 733, 873
Hin6I GCGC 2 cut(s) 222, 245
HinP1I GCGC 2 cut(s) 222, 245
HincII GTYRAC 3 cut(s) 477, 655, 1633
HindII GTYRAC 3 cut(s) 477, 655, 1633
HindIII AAGCTT 4 cut(s) 211, 708, 781, 1053
HinfI GANTC 4 cut(s) 407, 461, 1598, 1634
HpaII CCGG 5 cut(s) 195, 201, 291, 1388, 1527
HphI GGTGA 3 cut(s) 877, 1121, 1457
Hpy166II GTNNAC 8 cut(s) 118, 277, 477, 532, 655, 1001, 1421, 1633
Hpy188I TCNGA 3 cut(s) 104, 1137, 1542
Hpy188III TCNNGA 7 cut(s) 233, 411, 647, 719, 752, 1430, 1585
Hpy8I GTNNAC 8 cut(s) 118, 277, 477, 532, 655, 1001, 1421, 1633
Hpy99I CGWCG 4 cut(s) 44, 47, 478, 481
HpyAV CCTTC 3 cut(s) 540, 1391, 1501
HpyCH4III ACNGT 4 cut(s) 188, 274, 850, 1231
HpyCH4IV ACGT 1 cut(s) 601
HpyF3I CTNAG 8 cut(s) 101, 542, 779, 784, 1287, 1415, 1500, 1541
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 2 cut(s) 733, 873
HspAI GCGC 2 cut(s) 222, 245
Kzo9I GATC 3 cut(s) 643, 1410, 1456
LmnI GCTCC 6 cut(s) 20, 695, 915, 1125, 1209, 1496
LweI GCATC 4 cut(s) 33, 376, 553, 745
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 4 cut(s) 336, 577, 844, 865
MalI GATC 3 cut(s) 645, 1412, 1458
MbiI CCGCTC 1 cut(s) 59
MboI GATC 3 cut(s) 643, 1410, 1456
MboII GAAGA 8 cut(s) 426, 605, 806, 887, 980, 1292, 1487, 1559
MfeI CAATTG 1 cut(s) 1329
MflI RGATCY 2 cut(s) 643, 1410
MhlI GDGCHC 4 cut(s) 1144, 1214, 1374, 1488
MluCI AATT 5 cut(s) 381, 570, 984, 1082, 1329
MlyI GAGTC 3 cut(s) 470, 1592, 1628
MmeI TCCRAC 2 cut(s) 1081, 1213
MroXI GAANNNNTTC 2 cut(s) 603, 634
MseI TTAA 4 cut(s) 822, 899, 936, 1604
MslI CAYNNNNRTG 1 cut(s) 1006
MspA1I CMGCKG 2 cut(s) 1037, 1655
MspI CCGG 5 cut(s) 195, 201, 291, 1388, 1527
MspR9I CCNGG 4 cut(s) 195, 704, 1005, 1353
MunI CAATTG 1 cut(s) 1329
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 3 cut(s) 704, 1005, 1353
MvnI CGCG 3 cut(s) 222, 335, 981
NciI CCSGG 1 cut(s) 195
NdeII GATC 3 cut(s) 643, 1410, 1456
NlaIII CATG 2 cut(s) 733, 873
NlaIV GGNNCC 3 cut(s) 167, 199, 645
NmuCI GTSAC 3 cut(s) 336, 844, 865
NsbI TGCGCA 1 cut(s) 246
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 1 cut(s) 811
PdmI GAANNNNTTC 2 cut(s) 603, 634
PfeI GAWTC 1 cut(s) 407
PleI GAGTC 3 cut(s) 469, 1592, 1628
PpsI GAGTC 3 cut(s) 469, 1592, 1628
Psp124BI GAGCTC 2 cut(s) 1144, 1214
Psp1406I AACGTT 1 cut(s) 601
Psp6I CCWGG 3 cut(s) 702, 1003, 1351
PspFI CCCAGC 3 cut(s) 535, 961, 1157
PspGI CCWGG 3 cut(s) 702, 1003, 1351
PspN4I GGNNCC 3 cut(s) 167, 199, 645
PspPI GGNCC 4 cut(s) 165, 188, 217, 998
PstNI CAGNNNCTG 1 cut(s) 18
PsuI RGATCY 2 cut(s) 643, 1410
RsaI GTAC 4 cut(s) 486, 1228, 1380, 1422
RsaNI GTAC 4 cut(s) 485, 1227, 1379, 1421
RseI CAYNNNNRTG 1 cut(s) 1006
SacI GAGCTC 2 cut(s) 1144, 1214
SalI GTCGAC 1 cut(s) 475
SaqAI TTAA 4 cut(s) 822, 899, 936, 1604
Sau3AI GATC 3 cut(s) 643, 1410, 1456
Sau96I GGNCC 4 cut(s) 165, 188, 217, 998
ScaI AGTACT 1 cut(s) 1228
SchI GAGTC 3 cut(s) 470, 1592, 1628
ScrFI CCNGG 4 cut(s) 195, 704, 1005, 1353
SduI GDGCHC 4 cut(s) 1144, 1214, 1374, 1488
SfaNI GCATC 4 cut(s) 33, 376, 553, 745
SfcI CTRYAG 4 cut(s) 19, 69, 816, 945
SgrDI CGTCGACG 1 cut(s) 475
SinI GGWCC 3 cut(s) 188, 217, 998
SmiMI CAYNNNNRTG 1 cut(s) 1006
SmlI CTYRAG 2 cut(s) 614, 1213
SmoI CTYRAG 2 cut(s) 614, 1213
Sse9I AATT 5 cut(s) 381, 570, 984, 1082, 1329
SspI AATATT 1 cut(s) 941
SstI GAGCTC 2 cut(s) 1144, 1214
StyD4I CCNGG 4 cut(s) 193, 702, 1003, 1351
StyI CCWWGG 2 cut(s) 666, 861
TaaI ACNGT 4 cut(s) 188, 274, 850, 1231
TaiI ACGT 1 cut(s) 604
TaqI TCGA 5 cut(s) 410, 476, 1348, 1402, 1455
TaqII GACCGA 1 cut(s) 143
TasI AATT 5 cut(s) 381, 570, 984, 1082, 1329
TatI WGTACW 3 cut(s) 1226, 1378, 1420
TauI GCSGC 5 cut(s) 32, 78, 335, 1037, 1655
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 4 cut(s) 822, 899, 936, 1604
Tru9I TTAA 4 cut(s) 822, 899, 936, 1604
TscAI CASTG 5 cut(s) 231, 315, 546, 1498, 1567
TseFI GTSAC 3 cut(s) 336, 844, 865
Tsp45I GTSAC 3 cut(s) 336, 844, 865
TspDTI ATGAA 3 cut(s) 393, 612, 1451
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 5 cut(s) 231, 315, 546, 1498, 1567
VpaK11BI GGWCC 3 cut(s) 188, 217, 998
XapI RAATTY 1 cut(s) 984
XcmI CCANNNNNNNNNTGG 1 cut(s) 517
XmiI GTMKAC 3 cut(s) 117, 476, 531
XmnI GAANNNNTTC 2 cut(s) 603, 634
ZrmI AGTACT 1 cut(s) 1228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.