MD15G1312500.v1.1

SNF1-related protein kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003241
Physical Location & Seq
Forward (+)
31521055 .. 31521480
426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1312500.v1.1.491

Sequence Viewer

Length: 426 bp
ATGGGGAATGTGAATGGGAAAGAAGATGAGGATGGAAGCCCCTCAGCAGCCGAGGAAGATAGCGGTGCCGCTGGTAGCTTGCAGGAGGGCCCTACCTACGGCGTCTGTCTTGTTGATGGGTCAATCCCTCCTCATAGCCCTAGGGCCACTCACTCCCCTTTGATGTTCACTCCCCAAGTCCCTGTTGTCTCCTTACAAAGACCAGATGAGATACAAATTCCAAATAGCTTGTGGATGCAAACGTCGGGGTTTGAAAACATGTGCTGGGAGCAAGGAATTCCAACTATGATTACATGGAGCTATGGTGGGAAAGATGTAGTTGTGGAGGGATCATGGGATAATTGGAAGACAAGATTGGCTTTGCAGAGATCAGGAAAGGACTTCACTATTATGAAGGTAGCCTCTGTCTTTTCCCGTGAGTTGTAA

Protein Analysis

142

Amino Acids

15.39

Weight (kDa)

4.59

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 93 - 132 1.5e-12 Glycogen recognition site of AMP-activated protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AciI CCGC 2 cut(s) 63, 69
AclWI GGATC 1 cut(s) 337
AcsI RAATTY 2 cut(s) 216, 276
AcyI GRCGYC 1 cut(s) 102
AfiI CCNNNNNNNGG 1 cut(s) 98
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 1 cut(s) 254
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AluBI AGCT 3 cut(s) 78, 228, 300
AluI AGCT 3 cut(s) 78, 228, 300
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 1 cut(s) 337
AoxI GGCC 2 cut(s) 88, 144
ApaI GGGCCC 1 cut(s) 92
ApeKI GCWGC 1 cut(s) 47
ApoI RAATTY 2 cut(s) 216, 276
AspA2I CCTAGG 1 cut(s) 140
AspS9I GGNCC 3 cut(s) 88, 89, 144
AvrII CCTAGG 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 92
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 92
BbsI GAAGAC 1 cut(s) 353
BbvCI CCTCAGC 1 cut(s) 43
BbvI GCAGC 1 cut(s) 59
BccI CCATC 2 cut(s) 26, 110
BceAI ACGGC 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 1 cut(s) 141
BisI GCNGC 2 cut(s) 48, 69
BlnI CCTAGG 1 cut(s) 140
BlsI GCNGC 2 cut(s) 49, 70
BmgT120I GGNCC 3 cut(s) 88, 89, 144
BmiI GGNNCC 2 cut(s) 67, 90
BmsI GCATC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 353
Bpu10I CCTNAGC 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 51, 140
BsaXI ACNNNNNCTCC 2 cut(s) 289, 319
Bsc4I CCNNNNNNNGG 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 51, 140
BseGI GGATG 2 cut(s) 37, 240
BseLI CCNNNNNNNGG 1 cut(s) 98
BseMII CTCAG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 120
BseSI GKGCMC 1 cut(s) 92
BseXI GCAGC 1 cut(s) 59
BseYI CCCAGC 1 cut(s) 264
BshFI GGCC 2 cut(s) 90, 146
BshNI GGYRCC 1 cut(s) 65
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 1 cut(s) 98
BsmAI GTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 164
BsnI GGCC 2 cut(s) 90, 146
Bsp120I GGGCCC 1 cut(s) 88
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 2 cut(s) 329, 368
BspACI CCGC 2 cut(s) 63, 69
BspANI GGCC 2 cut(s) 90, 146
BspCNI CTCAG 1 cut(s) 56
BspLI GGNNCC 2 cut(s) 67, 90
BspPI GGATC 1 cut(s) 337
BspT107I GGYRCC 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 51, 140
BssMI GATC 2 cut(s) 329, 368
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 140
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 80
BstDEI CTNAG 1 cut(s) 43
BstF5I GGATG 2 cut(s) 37, 240
BstKTI GATC 2 cut(s) 332, 371
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 2 cut(s) 329, 368
BstNSI RCATGY 1 cut(s) 262
BstSLI GKGCMC 1 cut(s) 92
BstV1I GCAGC 1 cut(s) 59
BstV2I GAAGAC 1 cut(s) 353
BsuRI GGCC 2 cut(s) 90, 146
BtsCI GGATG 2 cut(s) 37, 240
Cac8I GCNNGC 1 cut(s) 80
Cfr13I GGNCC 3 cut(s) 88, 89, 144
CseI GACGC 1 cut(s) 91
CviAII CATG 3 cut(s) 259, 294, 333
DdeI CTNAG 1 cut(s) 43
DpnI GATC 2 cut(s) 331, 370
DpnII GATC 2 cut(s) 329, 368
Eco130I CCWWGG 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 92
EcoO109I RGGNCCY 2 cut(s) 88, 89
EcoRI GAATTC 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 140
EcoT38I GRGCYC 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 140
FaeI CATG 3 cut(s) 262, 297, 336
FaiI YATR 7 cut(s) 135, 260, 287, 295, 303, 334, 392
FalI AAGNNNNNCTT 2 cut(s) 343, 375
FaqI GGGAC 1 cut(s) 164
FatI CATG 3 cut(s) 258, 293, 332
Fnu4HI GCNGC 2 cut(s) 48, 69
FokI GGATG 2 cut(s) 44, 247
FriOI GRGCYC 1 cut(s) 92
Fsp4HI GCNGC 2 cut(s) 48, 69
FspBI CTAG 1 cut(s) 141
GluI GCNGC 2 cut(s) 48, 69
GsaI CCCAGC 1 cut(s) 268
HaeIII GGCC 2 cut(s) 90, 146
HgaI GACGC 1 cut(s) 91
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 3 cut(s) 262, 297, 336
Hpy166II GTNNAC 1 cut(s) 168
Hpy188III TCNNGA 1 cut(s) 372
Hpy8I GTNNAC 1 cut(s) 168
Hpy99I CGWCG 1 cut(s) 247
HpyAV CCTTC 1 cut(s) 388
HpyCH4IV ACGT 1 cut(s) 242
HpyCH4V TGCA 3 cut(s) 82, 238, 364
HpyF3I CTNAG 1 cut(s) 43
HpySE526I ACGT 1 cut(s) 242
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 3 cut(s) 262, 297, 336
Kzo9I GATC 2 cut(s) 329, 368
LmnI GCTCC 2 cut(s) 268, 297
LpnPI CCDG 6 cut(s) 57, 68, 195, 216, 250, 357
Lsp1109I GCAGC 1 cut(s) 59
LweI GCATC 1 cut(s) 225
MaeI CTAG 1 cut(s) 141
MaeII ACGT 1 cut(s) 242
MalI GATC 2 cut(s) 331, 370
MboI GATC 2 cut(s) 329, 368
MboII GAAGA 3 cut(s) 35, 68, 358
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 3 cut(s) 216, 276, 340
MmeI TCCRAC 1 cut(s) 305
MnlI CCTC 8 cut(s) 22, 46, 52, 79, 138, 141, 319, 412
MslI CAYNNNNRTG 1 cut(s) 389
MspA1I CMGCKG 1 cut(s) 71
NdeII GATC 2 cut(s) 329, 368
NlaIII CATG 3 cut(s) 262, 297, 336
NlaIV GGNNCC 2 cut(s) 67, 90
NmeAIII GCCGAG 1 cut(s) 76
NspI RCATGY 1 cut(s) 262
PciI ACATGT 1 cut(s) 258
PkrI GCNGC 2 cut(s) 49, 70
PscI ACATGT 1 cut(s) 258
PspFI CCCAGC 1 cut(s) 264
PspN4I GGNNCC 2 cut(s) 67, 90
PspOMI GGGCCC 1 cut(s) 88
PspPI GGNCC 3 cut(s) 88, 89, 144
RseI CAYNNNNRTG 1 cut(s) 389
SatI GCNGC 2 cut(s) 48, 69
Sau3AI GATC 2 cut(s) 329, 368
Sau96I GGNCC 3 cut(s) 88, 89, 144
SduI GDGCHC 1 cut(s) 92
SetI ASST 6 cut(s) 80, 98, 230, 245, 302, 399
SfaNI GCATC 1 cut(s) 225
SmiMI CAYNNNNRTG 1 cut(s) 389
Sse9I AATT 3 cut(s) 216, 276, 340
SsiI CCGC 2 cut(s) 63, 69
SspMI CTAG 1 cut(s) 141
StyI CCWWGG 1 cut(s) 140
TaiI ACGT 1 cut(s) 245
TasI AATT 3 cut(s) 216, 276, 340
TauI GCSGC 1 cut(s) 71
TseI GCWGC 1 cut(s) 47
TspDTI ATGAA 1 cut(s) 407
XapI RAATTY 2 cut(s) 216, 276
XceI RCATGY 1 cut(s) 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 228
XmaJI CCTAGG 1 cut(s) 140
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.