Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003227

Physical Location & Seq

Genomic Coordinates Forward (+)

11772293 .. 11793872

Gene Structure 21580 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1129800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3594 bp

ATGTGGGCGTCGCAGAGAATCATCTATGCTCTCGCTCTCGCTCATCCCCTCTCGTCTTCGTCAGCCTTCGTTTCCGGAATTTTCCTCTTCCTCCGGTCCTCCTCTCCTTCCTCCGCCCATCCTCGCTTTCTCCCTCCCTCGCTTTCTCTCTCCTCTTCCATCTCGCTCCCCCATTCCAGCATGCCTTACAACCAGCAAAGGGGTGGGCGTACAGAACAAAAGTGGACACAAAAGCAAAAGACTGACAGCCCGTCAATGTCCACTGGGGATTCTTCAGCAGCTGTCACTAACAGAGTTGGTGAGTTGAGTCTTACTGGAAGCAATGGGCAGGCCAATGCTTCAGTTGAACCCACACAGTTTGGGAAGGTCCAATTGGCAAATCAGGGTCCAGTTCAAGGGCAGAAAGGAATTTGGATGCCTAAATCATATGTGACAGTGAGCGGAGCTAAGGCGGTAGAAGTTGAAGCAAATGTTGATGAAAGTACAGTTGGAACCCAAGGAAATGGAGCTGGACAAGCAGCAGCTCAAAAAACCAGAGTTGGTTTAAGTAAGCTGTTCAATGGTAATCTGTTGGAAAATTTTACTGTGGACAACTCCACTTATGCTCAGGTGCAAATACGAGCTACTTTCTATCCCAAATTTGAAAATGAGAAGACAGATGGGGAGATTAGGACAAGGATGATAGAGATGGTATCTAACGGCTTGGCTACCTTGGAGGTTTCACTTAAACACTCAGGGTCTCTTTTTATGTACGCCGGTAATAAGGGTGGGGCATATGCCAAAAACAGCTTTGGGAATATATATACGGCTGTAGGTGTATTTGTTCTTGGACGGATGTTTCGGGAGGCATGGGGACGTGAAGCACCAAAAATGCAAGAAGAGTTTAATGATTTCCTTGAGAGGAATCGTATGTGCATATCAATGGAACTTGTAACTGCTGTTCTAGGAGACCATGGACAACGCCCGAAAGAAGATTTTGCTGTAGTGACGGCAGTCACAGATTTGGGCAATGGAAAGCCAAAGTTCTACTCAACCCCTGAGATAATTGCTTTCTGTAGAAAATGGCGCCTACCAACAAATCATGTATGGCTGTTCTCAACAAGGAAATCAGTGACATCATTTTTTGCTGCCTTTGATGCACTCTGCGAAGAAGGAACTGCAACACCTGTATGCATGGCTCTTAATGAAGTTGCAGATATATCTATACCAGGTTCAAAAGATCATGTAAAGGAGCAGGGTGAAATCTTAGAGGGCATTGTTGCTCGTATTGTAAGCCAGGAAAGCTCAGAACATGTGGAAAAAGTCTTGAAAGATTTCCCTCCTCCACCAATCGATGGAGCTGGTCTTGATTTGGGACCGAGCGCACGAGATATTTGTGCTGCAAATAGGTCGAGTGAAAAGCAGCAAATAGAAGCGCTTCTTGAGGGTGTTGGCTCCTCTTTTTGCCCTGACCATTCGGACTGGTTTGGAGTTGGAGCTGGTGATGACCATTCCAGAAACGCAGATAAATCTGTGCTATCAAAACTTTTGCAATCTCATCCTGCAGATTTTTCTACCACCAAATTGCAGGAAATGATTCGGTTAATGAAAGAAAAGCGTTTTCCTGCTGCCTTTAAATGTTACCATGACTACCACAAAATTGATTCCATATCAAATGACAACCTTTTCTATAAAATGGTCATCCATGTGCATAGTGACTCCGCATTTAGGCGTTACCAGAAAGAGATGAGGTCTAAGCCAGGACTGTGGCCTTTATATCGAGGTTTTTTTGTTGACATTAATTTATTCAAGGCAAGCAAGGAGAGAGCTGCTGAGATAGCAAAAGGCAAAAGCAGCATTGTGGAAGATGGGAACAATGGTATGCCTGGAAAATATGGATTGGCTGATGAAGATGCAAACTTAATGATAAAACTGAAATTTCTTACATACAAGCTGCGAACCTTTTTGATTCGCAATGGTTTGTCAATTCTGTTTAAAGAAGGTACAGCTGCTTACAAGACCTATTACGAGCGGCAAATGAAAATATGGAATACTTCAGAACCAAAGAAGAGGGAACTCAGAAAGATGCTTGATGAATGGGCTGTGTACATACGAAGGAAATGTGGAAACAAACCGCTTTCATCATCAGTTTACCTCAGTGAGGCTGAGCCTTTTCTTGAACAGTATGCAAAACGCAGTCCACAGAATCAGTCTCTCATTGGATCTGCTGGAAACTTTGTAAGGGCTGAAGATTTCCTGGCCATTGTTGAGGGAGGAAGAGATGAAGAGGGTGATCTTGAGAGGGAGCAGGAGGTTGCACCATCAAGCCCCAGTGCCTCAGGCAGGGACACTATCCCAAAGGCTGAGGGTCTGATTGTATTCTTTCCTGGACTACCTGGTTCTGCTAAGTCAGCATTATGCAAGGAACTACTTCAAGACCCAGGAAGAATGGGAGATGATCGACCAATTCAAAGCCTGATGGGTGACCTTATCAAAGGAAAATATTGGCAGAAGGTTGCTGAAGAGCGCAAGAAAAAACCATATTCCATAATGCTTGCAGACAAGAATGCACCAAATGAAGAAGTATGGAGACAGATTGAACACATGTGCAACAGCACTAGGGCCTCCGCGGTTCCAGTCGTGCCTGATTCTGAAGGAACCGATTCAAATCCATTTTCTCTTGATGCATTAGCAATTTTCATGTTTCGGGTGCTTCAACGTGCTAATCATCCAGGAAACCTTGACAAGGAATCTTTAAATGCTGGCTATGTGCTGCTAATGTTCTATCACCTTTACGAGGGCAAGAGTCGCCGAGAGTTTGATGGGGAATTAGTTGAGCGTTTTGGCTCACTTGTAAAGATGCCGTTGCTGAAGTCTGATAGGAATCCATTACCTGATCCCGTGAAGAATATTCTGGAGGAGGGAATAAATCTATACAAGCTTCACACCGCCAAACATGGAAGATTGGAGTCGACCAAGGGAACCTATGCAAAGGAGTGGGCTAAATGGGAGAAACAGTTGCGGGATATTTTATTTGGCAATGCTGAGTATCTGAATTCTGTTCAGGTTCCTTTTGAGTCTGCTGTAAAGGAAGTGTCAGAACAGCTGAAAAAGGTCGCAAAAGGTGAGTACAAAACTCCAGATACTGGGAAGAGGAAGTTCGGCGCCATTGTCTTTGCTGCCGTTAGCCTGCCTGTTATGGAAGTCAGTGGTCTGCTTGATAGCTTGGCTGTGAAACATAGTGAAGCCGAAGCTTTCCTCAAAGACAAGCATTTGGATAAACTTAACAAGGCTCATGTTACTCTTGCACACAAGAGAAGTCATGGTGTTACTGCCGTGGCTAGTTACGGAACCTTCCTTCACAAAACGGTCCCTGTGGATATAACTAAGCTTTTCTTCTCGGATAAAATGGCTGCATTTGGAGCCAGCCTTGGGTCTGTCGAAGGTGAAAGAGTAGTGTCTAAAAATGAGTGGCCGCATGTCACCTTATGGACTGCAGAAGGGGTTCCGGCCAAGGACGCCAACAACTTACCCCAATTGCATTCGGAGGGGAAAGCTACCTGCATTGCAATTGACCCACCTGCCACCATTGATGGCACGCTGGAATTTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000394 GO:0000910 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003972 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006388 GO:0006396 GO:0006399 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008033 GO:0008150 GO:0008152 GO:0008356 GO:0008380 GO:0008452 GO:0009790 GO:0009791 GO:0009793 GO:0009966 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010467 GO:0010646 GO:0010928 GO:0016070 GO:0016874 GO:0016886 GO:0022402 GO:0022414 GO:0023051 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034660 GO:0042245 GO:0043170 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048316 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051301 GO:0061458 GO:0061640 GO:0065007 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

tRNA_lig_CPD

Protein Analysis

1198

Amino Acids

132.55

Weight (kDa)

8.47

Isoelectric Point (pI)

37.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_lig_CPD	PF08302	1022 - 1183	8.4e-06	Fungal tRNA ligase phosphodiesterase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07910 AT1G07910
fragaria_vesca	FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_4g02320
malus_domestica	MD03G1112400.v1.1 MD11G1129800.v1.1
prunus_persica	Prupe.6G094700_v2.0.a1
pyrus_communis	pycom11g10750 pycom11g10760
rosa_chinensis	RchiOBHm_Chr4g0389641 RchiOBHm_Chr5g0060751
rosa_laevigata	RLG00000009964 RLG00000035390
rosa_multiflora	Rmu_co8520825.1_g000001 Rmu_sc0006568.1_g000004 Rmu_sc0007419.1_g000006
rosa_roxburghii	Rroxscaffold_1G00019960 Rroxscaffold_5G00335630
rosa_rugosa	Rorug03G0324600 Rorug05G0333400
rosa_samantha	Rh4AG029200 Rh4BG022400 Rh4CG031200 Rh4DG022900 Rh5AG396400 Rh5BG408600 Rh5CG433200 Rh5DG423800
rosa_wichuraiana	Rw4G002180 Rw5G037370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3568
Acc36I	ACCTGC	2 cut(s)	3548, 3568
AccB1I	GGYRCC	2 cut(s)	1065, 3143
AccB7I	CCANNNNNTGG	2 cut(s)	1334, 3125
AccBSI	CCGCTC	2 cut(s)	441, 2011
AccI	GTMKAC	1 cut(s)	2951
AccII	CGCG	1 cut(s)	2609
AccIII	TCCGGA	1 cut(s)	74
AclWI	GGATC	2 cut(s)	2209, 2870
AcoI	YGGCCR	3 cut(s)	2238, 3452, 3489
AcsI	RAATTY	7 cut(s)	78, 408, 577, 638, 1916, 3034, 3584
AcuI	CTGAAG	7 cut(s)	258, 324, 2019, 2247, 2520, 2652, 2870
AcyI	GRCGYC	4 cut(s)	8, 1066, 3144, 3498
AfaI	GTAC	6 cut(s)	211, 484, 752, 1984, 2087, 3110
AfeI	AGCGCT	1 cut(s)	1416
AflIII	ACRYGT	2 cut(s)	1291, 2583
AhdI	GACNNNNNGTC	1 cut(s)	250
AjiI	CACGTC	1 cut(s)	857
AjnI	CCWGG	9 cut(s)	1207, 1275, 1738, 1864, 2235, 2365, 2374, 2419, 2710
AjuI	GAANNNNNNNTTGG	4 cut(s)	356, 388, 471, 503
Alw26I	GTCTC	4 cut(s)	744, 942, 2196, 2563
AlwI	GGATC	2 cut(s)	2209, 2870
AlwNI	CAGNNNCTG	2 cut(s)	281, 3125
Aor13HI	TCCGGA	1 cut(s)	74
Aor51HI	AGCGCT	1 cut(s)	1416
AoxI	GGCC	6 cut(s)	330, 1748, 2238, 2601, 3452, 3489
ApoI	RAATTY	7 cut(s)	78, 408, 577, 638, 1916, 3034, 3584
ArsI	GACNNNNNNTTYG	2 cut(s)	822, 854
AseI	ATTAAT	1 cut(s)	1779
Asp700I	GAANNNNTTC	6 cut(s)	1313, 1416, 1575, 2409, 2641, 3483
AspLEI	GCGC	5 cut(s)	1068, 1364, 1417, 2508, 3146
AspS9I	GGNCC	6 cut(s)	96, 367, 386, 1355, 2601, 3349
AsuHPI	GGTGA	9 cut(s)	311, 1250, 1493, 2282, 2474, 2759, 3116, 3437, 3454
AvaII	GGWCC	5 cut(s)	96, 367, 386, 1355, 3349
AxyI	CCTNAGG	1 cut(s)	2317
BalI	TGGCCA	1 cut(s)	2240
BanI	GGYRCC	2 cut(s)	1065, 3143
BauI	CACGAG	1 cut(s)	1365
BbsI	GAAGAC	2 cut(s)	48, 659
BbvCI	CCTCAGC	1 cut(s)	2343
BceAI	ACGGC	6 cut(s)	715, 822, 1005, 2827, 3146, 3299
BcgI	CGANNNNNNTGC	2 cut(s)	1371, 1405
BciT130I	CCWGG	9 cut(s)	1209, 1277, 1740, 1866, 2237, 2367, 2376, 2421, 2712
BcoDI	GTCTC	4 cut(s)	744, 942, 2196, 2563
BfaI	CTAG	3 cut(s)	944, 2598, 3321
BfmI	CTRYAG	5 cut(s)	810, 981, 1054, 1542, 3474
BfoI	RGCGCY	3 cut(s)	1069, 1418, 3147
BfuAI	ACCTGC	2 cut(s)	3548, 3568
BlpI	GCTNAGC	1 cut(s)	2145
Bme1390I	CCNGG	9 cut(s)	1209, 1277, 1740, 1866, 2237, 2367, 2376, 2421, 2712
Bme18I	GGWCC	5 cut(s)	96, 367, 386, 1355, 3349
BmeRI	GACNNNNNGTC	1 cut(s)	250
BmgBI	CACGTC	1 cut(s)	857
BmgT120I	GGNCC	6 cut(s)	96, 367, 386, 1355, 2601, 3349
BmrFI	CCNGG	9 cut(s)	1209, 1277, 1740, 1866, 2237, 2367, 2376, 2421, 2712
BmrI	ACTGGG	3 cut(s)	273, 2304, 3135
BmsI	GCATC	6 cut(s)	405, 1126, 1882, 2055, 2653, 2829
BmuI	ACTGGG	3 cut(s)	273, 2304, 3135
BoxI	GACNNNNGTC	1 cut(s)	992
BpiI	GAAGAC	2 cut(s)	48, 659
BpmI	CTGGAG	2 cut(s)	2915, 3102
Bpu10I	CCTNAGC	3 cut(s)	447, 606, 2343
Bpu1102I	GCTNAGC	1 cut(s)	2145
BpuEI	CTTGAG	3 cut(s)	917, 1442, 2297
Bsa29I	ATCGAT	1 cut(s)	1332
BsaBI	GATNNNNATC	2 cut(s)	2646, 2862
BsaHI	GRCGYC	4 cut(s)	8, 1066, 3144, 3498
BsaI	GGTCTC	2 cut(s)	744, 942
BsaJI	CCNNGG	9 cut(s)	496, 711, 952, 2419, 2607, 2955, 3315, 3409, 3492
BsaWI	WCCGGW	2 cut(s)	74, 93
BsaXI	ACNNNNNCTCC	2 cut(s)	2981, 3011
Bse118I	RCCGGY	1 cut(s)	755
Bse1I	ACTGG	7 cut(s)	268, 319, 389, 1466, 2310, 2615, 3130
Bse21I	CCTNAGG	1 cut(s)	2317
Bse3DI	GCAATG	5 cut(s)	328, 1015, 1960, 3025, 3543
Bse8I	GATNNNNATC	2 cut(s)	2646, 2862
BseAI	TCCGGA	1 cut(s)	74
BseBI	CCWGG	9 cut(s)	1209, 1277, 1740, 1866, 2237, 2367, 2376, 2421, 2712
BseCI	ATCGAT	1 cut(s)	1332
BseDI	CCNNGG	9 cut(s)	496, 711, 952, 2419, 2607, 2955, 3315, 3409, 3492
BseGI	GGATG	8 cut(s)	43, 118, 420, 684, 840, 1537, 1680, 2707
BseJI	GATNNNNATC	2 cut(s)	2646, 2862
BseMI	GCAATG	5 cut(s)	328, 1015, 1960, 3025, 3543
BseNI	ACTGG	7 cut(s)	268, 319, 389, 1466, 2310, 2615, 3130
BseRI	GAGGAG	5 cut(s)	91, 142, 1311, 1426, 2912
Bsh1236I	CGCG	1 cut(s)	2609
BshFI	GGCC	6 cut(s)	332, 1750, 2240, 2603, 3454, 3491
BshNI	GGYRCC	2 cut(s)	1065, 3143
BshVI	ATCGAT	1 cut(s)	1332
BsiSI	CCGG	4 cut(s)	75, 94, 756, 3488
BslFI	GGGAC	4 cut(s)	867, 1368, 2339, 3335
BsmAI	GTCTC	4 cut(s)	744, 942, 2196, 2563
BsmFI	GGGAC	4 cut(s)	867, 1368, 2339, 3335
BsmI	GAATGC	2 cut(s)	2551, 3520
BsnI	GGCC	6 cut(s)	332, 1750, 2240, 2603, 3454, 3491
Bso31I	GGTCTC	2 cut(s)	744, 942
Bsp13I	TCCGGA	1 cut(s)	74
Bsp1407I	TGTACA	1 cut(s)	2085
Bsp143I	GATC	5 cut(s)	1219, 2201, 2272, 2437, 2875
Bsp1720I	GCTNAGC	1 cut(s)	2145
Bsp19I	CCATGG	1 cut(s)	952
BspANI	GGCC	6 cut(s)	332, 1750, 2240, 2603, 3454, 3491
BspDI	ATCGAT	1 cut(s)	1332
BspEI	TCCGGA	1 cut(s)	74
BspFNI	CGCG	1 cut(s)	2609
BspMAI	CTGCAG	2 cut(s)	1546, 3478
BspMI	ACCTGC	2 cut(s)	3548, 3568
BspPI	GGATC	2 cut(s)	2209, 2870
BspQI	GCTCTTC	1 cut(s)	2496
BspT107I	GGYRCC	2 cut(s)	1065, 3143
BspTNI	GGTCTC	2 cut(s)	744, 942
BsrBI	CCGCTC	2 cut(s)	441, 2011
BsrDI	GCAATG	5 cut(s)	328, 1015, 1960, 3025, 3543
BsrFI	RCCGGY	1 cut(s)	755
BsrGI	TGTACA	1 cut(s)	2085
BsrI	ACTGG	7 cut(s)	268, 319, 389, 1466, 2310, 2615, 3130
BssAI	RCCGGY	1 cut(s)	755
BssECI	CCNNGG	9 cut(s)	496, 711, 952, 2419, 2607, 2955, 3315, 3409, 3492
BssMI	GATC	5 cut(s)	1219, 2201, 2272, 2437, 2875
BssNI	GRCGYC	4 cut(s)	8, 1066, 3144, 3498
BssSI	CACGAG	1 cut(s)	1365
BssT1I	CCWWGG	6 cut(s)	496, 711, 952, 2955, 3409, 3492
Bst2BI	CACGAG	1 cut(s)	1365
Bst2UI	CCWGG	9 cut(s)	1209, 1277, 1740, 1866, 2237, 2367, 2376, 2421, 2712
Bst4CI	ACNGT	8 cut(s)	357, 436, 487, 586, 1746, 2163, 2997, 3349
Bst6I	CTCTTC	8 cut(s)	92, 160, 873, 2042, 2251, 2259, 2496, 3125
BstACI	GRCGYC	4 cut(s)	8, 1066, 3144, 3498
BstAUI	TGTACA	1 cut(s)	2085
BstC8I	GCNNGC	8 cut(s)	182, 330, 1795, 2535, 2743, 3170, 3406, 3578
BstDSI	CCRYGG	3 cut(s)	952, 2607, 3315
BstEII	GGTNACC	1 cut(s)	2462
BstENI	CCTNNNNNAGG	4 cut(s)	2138, 2320, 2723, 2774
BstF5I	GGATG	8 cut(s)	43, 118, 420, 684, 840, 1537, 1680, 2707
BstFNI	CGCG	1 cut(s)	2609
BstH2I	RGCGCY	3 cut(s)	1069, 1418, 3147
BstHHI	GCGC	5 cut(s)	1068, 1364, 1417, 2508, 3146
BstKTI	GATC	5 cut(s)	1222, 2204, 2275, 2440, 2878
BstMAI	GTCTC	4 cut(s)	744, 942, 2196, 2563
BstMBI	GATC	5 cut(s)	1219, 2201, 2272, 2437, 2875
BstMWI	GCNNNNNNNGC	9 cut(s)	515, 1136, 1281, 1817, 1833, 2390, 2787, 3401, 3497
BstNI	CCWGG	9 cut(s)	1209, 1277, 1740, 1866, 2237, 2367, 2376, 2421, 2712
BstNSI	RCATGY	4 cut(s)	184, 1295, 2587, 3461
BstPAI	GACNNNNGTC	1 cut(s)	992
BstPI	GGTNACC	1 cut(s)	2462
BstSCI	CCNGG	9 cut(s)	1207, 1275, 1738, 1864, 2235, 2365, 2374, 2419, 2710
BstSFI	CTRYAG	5 cut(s)	810, 981, 1054, 1542, 3474
BstUI	CGCG	1 cut(s)	2609
BstV2I	GAAGAC	2 cut(s)	48, 659
BstX2I	RGATCY	1 cut(s)	2201
BstXI	CCANNNNNNTGG	2 cut(s)	503, 1746
BstYI	RGATCY	1 cut(s)	2201
Bsu15I	ATCGAT	1 cut(s)	1332
Bsu36I	CCTNAGG	1 cut(s)	2317
BsuRI	GGCC	6 cut(s)	332, 1750, 2240, 2603, 3454, 3491
BsuTUI	ATCGAT	1 cut(s)	1332
BtgI	CCRYGG	3 cut(s)	952, 2607, 3315
BtrI	CACGTC	1 cut(s)	857
BtsCI	GGATG	8 cut(s)	43, 118, 420, 684, 840, 1537, 1680, 2707
BtsIMutI	CAGTG	6 cut(s)	261, 441, 1116, 2143, 2317, 3193
BveI	ACCTGC	2 cut(s)	3548, 3568
Cac8I	GCNNGC	8 cut(s)	182, 330, 1795, 2535, 2743, 3170, 3406, 3578
CaiI	CAGNNNCTG	2 cut(s)	281, 3125
CfoI	GCGC	5 cut(s)	1068, 1364, 1417, 2508, 3146
Cfr10I	RCCGGY	1 cut(s)	755
Cfr13I	GGNCC	6 cut(s)	96, 367, 386, 1355, 2601, 3349
Cfr42I	CCGCGG	1 cut(s)	2610
ClaI	ATCGAT	1 cut(s)	1332
CseI	GACGC	1 cut(s)	3506
CsiI	ACCWGGT	2 cut(s)	1207, 2374
Csp6I	GTAC	6 cut(s)	210, 483, 751, 1983, 2086, 3109
CspCI	CAANNNNNGTGG	2 cut(s)	340, 375
CviQI	GTAC	6 cut(s)	210, 483, 751, 1983, 2086, 3109
DinI	GGCGCC	2 cut(s)	1067, 3145
DpnI	GATC	5 cut(s)	1221, 2203, 2274, 2439, 2877
DpnII	GATC	5 cut(s)	1219, 2201, 2272, 2437, 2875
DraI	TTTAAA	3 cut(s)	1615, 1975, 2736
DriI	GACNNNNNGTC	1 cut(s)	250
EaeI	YGGCCR	3 cut(s)	2238, 3452, 3489
Eam1104I	CTCTTC	8 cut(s)	92, 160, 873, 2042, 2251, 2259, 2496, 3125
Eam1105I	GACNNNNNGTC	1 cut(s)	250
EarI	CTCTTC	8 cut(s)	92, 160, 873, 2042, 2251, 2259, 2496, 3125
EciI	GGCGGA	1 cut(s)	103
Eco130I	CCWWGG	6 cut(s)	496, 711, 952, 2955, 3409, 3492
Eco31I	GGTCTC	2 cut(s)	744, 942
Eco47I	GGWCC	5 cut(s)	96, 367, 386, 1355, 3349
Eco47III	AGCGCT	1 cut(s)	1416
Eco57I	CTGAAG	7 cut(s)	258, 324, 2019, 2247, 2520, 2652, 2870
Eco81I	CCTNAGG	1 cut(s)	2317
Eco91I	GGTNACC	1 cut(s)	2462
EcoNI	CCTNNNNNAGG	4 cut(s)	2138, 2320, 2723, 2774
EcoO109I	RGGNCCY	1 cut(s)	2601
EcoO65I	GGTNACC	1 cut(s)	2462
EcoRI	GAATTC	1 cut(s)	3034
EcoRII	CCWGG	9 cut(s)	1207, 1275, 1738, 1864, 2235, 2365, 2374, 2419, 2710
EcoT14I	CCWWGG	6 cut(s)	496, 711, 952, 2955, 3409, 3492
EcoT22I	ATGCAT	2 cut(s)	1175, 2668
EgeI	GGCGCC	2 cut(s)	1067, 3145
EheI	GGCGCC	2 cut(s)	1067, 3145
ErhI	CCWWGG	6 cut(s)	496, 711, 952, 2955, 3409, 3492
FalI	AAGNNNNNCTT	4 cut(s)	1404, 1436, 3359, 3391
FaqI	GGGAC	4 cut(s)	867, 1368, 2339, 3335
FauI	CCCGC	1 cut(s)	2994
FauNDI	CATATG	2 cut(s)	427, 775
FblI	GTMKAC	1 cut(s)	2951
FokI	GGATG	8 cut(s)	30, 105, 427, 691, 847, 1524, 1667, 2694
FspBI	CTAG	3 cut(s)	944, 2598, 3321
GlaI	GCGC	5 cut(s)	1067, 1363, 1416, 2507, 3145
GsuI	CTGGAG	2 cut(s)	2915, 3102
HaeII	RGCGCY	3 cut(s)	1069, 1418, 3147
HaeIII	GGCC	6 cut(s)	332, 1750, 2240, 2603, 3454, 3491
HapII	CCGG	4 cut(s)	75, 94, 756, 3488
HgaI	GACGC	1 cut(s)	3506
HhaI	GCGC	5 cut(s)	1068, 1364, 1417, 2508, 3146
Hin1I	GRCGYC	4 cut(s)	8, 1066, 3144, 3498
Hin6I	GCGC	5 cut(s)	1066, 1362, 1415, 2506, 3144
HinP1I	GCGC	5 cut(s)	1066, 1362, 1415, 2506, 3144
HincII	GTYRAC	2 cut(s)	1774, 2952
HindII	GTYRAC	2 cut(s)	1774, 2952
HindIII	AAGCTT	3 cut(s)	2918, 3231, 3368
HpaII	CCGG	4 cut(s)	75, 94, 756, 3488
HphI	GGTGA	9 cut(s)	311, 1250, 1493, 2282, 2474, 2759, 3116, 3437, 3454
Hpy166II	GTNNAC	8 cut(s)	225, 261, 589, 1774, 2086, 2131, 2180, 2952
Hpy8I	GTNNAC	8 cut(s)	225, 261, 589, 1774, 2086, 2131, 2180, 2952
Hpy99I	CGWCG	1 cut(s)	13
HpyCH4III	ACNGT	8 cut(s)	357, 436, 487, 586, 1746, 2163, 2997, 3349
HpyCH4IV	ACGT	2 cut(s)	856, 2698
HpyF10VI	GCNNNNNNNGC	9 cut(s)	515, 1136, 1281, 1817, 1833, 2390, 2787, 3401, 3497
HpySE526I	ACGT	2 cut(s)	856, 2698
Hsp92I	GRCGYC	4 cut(s)	8, 1066, 3144, 3498
HspAI	GCGC	5 cut(s)	1066, 1362, 1415, 2506, 3144
KasI	GGCGCC	2 cut(s)	1065, 3143
Kpn2I	TCCGGA	1 cut(s)	74
KspI	CCGCGG	1 cut(s)	2610
Kzo9I	GATC	5 cut(s)	1219, 2201, 2272, 2437, 2875
LguI	GCTCTTC	1 cut(s)	2496
LmnI	GCTCC	9 cut(s)	171, 443, 506, 1231, 1337, 1439, 1475, 2284, 3401
LweI	GCATC	6 cut(s)	405, 1126, 1882, 2055, 2653, 2829
MabI	ACCWGGT	2 cut(s)	1207, 2374
MaeI	CTAG	3 cut(s)	944, 2598, 3321
MaeII	ACGT	2 cut(s)	856, 2698
MalI	GATC	5 cut(s)	1221, 2203, 2274, 2439, 2877
MbiI	CCGCTC	2 cut(s)	441, 2011
MboI	GATC	5 cut(s)	1219, 2201, 2272, 2437, 2875
MfeI	CAATTG	3 cut(s)	371, 3515, 3549
MflI	RGATCY	1 cut(s)	2201
MlsI	TGGCCA	1 cut(s)	2240
MluNI	TGGCCA	1 cut(s)	2240
Mly113I	GGCGCC	2 cut(s)	1066, 3144
MlyI	GAGTC	5 cut(s)	316, 1691, 2794, 2957, 3065
MmeI	TCCRAC	3 cut(s)	469, 552, 1453
Mox20I	TGGCCA	1 cut(s)	2240
Mph1103I	ATGCAT	2 cut(s)	1175, 2668
MroI	TCCGGA	1 cut(s)	74
MroXI	GAANNNNTTC	6 cut(s)	1313, 1416, 1575, 2409, 2641, 3483
MscI	TGGCCA	1 cut(s)	2240
MslI	CAYNNNNRTG	3 cut(s)	920, 1168, 1685
Msp20I	TGGCCA	1 cut(s)	2240
MspA1I	CMGCKG	4 cut(s)	281, 1988, 2609, 3085
MspI	CCGG	4 cut(s)	75, 94, 756, 3488
MspR9I	CCNGG	9 cut(s)	1209, 1277, 1740, 1866, 2237, 2367, 2376, 2421, 2712
MunI	CAATTG	3 cut(s)	371, 3515, 3549
Mva1269I	GAATGC	2 cut(s)	2551, 3520
MvaI	CCWGG	9 cut(s)	1209, 1277, 1740, 1866, 2237, 2367, 2376, 2421, 2712
MvnI	CGCG	1 cut(s)	2609
MwoI	GCNNNNNNNGC	9 cut(s)	515, 1136, 1281, 1817, 1833, 2390, 2787, 3401, 3497
NarI	GGCGCC	2 cut(s)	1066, 3144
NcoI	CCATGG	1 cut(s)	952
NdeI	CATATG	2 cut(s)	427, 775
NdeII	GATC	5 cut(s)	1219, 2201, 2272, 2437, 2875
NmeAIII	GCCGAG	1 cut(s)	2816
NmuCI	GTSAC	8 cut(s)	283, 430, 985, 994, 1111, 1694, 2462, 3460
NsiI	ATGCAT	2 cut(s)	1175, 2668
NspI	RCATGY	4 cut(s)	184, 1295, 2587, 3461
PaeI	GCATGC	1 cut(s)	184
PaqCI	CACCTGC	1 cut(s)	3568
PciI	ACATGT	2 cut(s)	1291, 2583
PciSI	GCTCTTC	1 cut(s)	2496
PctI	GAATGC	2 cut(s)	2551, 3520
PdmI	GAANNNNTTC	6 cut(s)	1313, 1416, 1575, 2409, 2641, 3483
PflMI	CCANNNNNTGG	2 cut(s)	1334, 3125
PfoI	TCCNGGA	2 cut(s)	2365, 2710
PleI	GAGTC	5 cut(s)	315, 1691, 2793, 2956, 3064
PluTI	GGCGCC	2 cut(s)	1069, 3147
PpsI	GAGTC	5 cut(s)	315, 1691, 2793, 2956, 3064
PscI	ACATGT	2 cut(s)	1291, 2583
PshAI	GACNNNNGTC	1 cut(s)	992
PshBI	ATTAAT	1 cut(s)	1779
Psp6I	CCWGG	9 cut(s)	1207, 1275, 1738, 1864, 2235, 2365, 2374, 2419, 2710
PspEI	GGTNACC	1 cut(s)	2462
PspGI	CCWGG	9 cut(s)	1207, 1275, 1738, 1864, 2235, 2365, 2374, 2419, 2710
PspPI	GGNCC	6 cut(s)	96, 367, 386, 1355, 2601, 3349
PsrI	GAACNNNNNNTAC	2 cut(s)	924, 956
PstI	CTGCAG	2 cut(s)	1546, 3478
PstNI	CAGNNNCTG	2 cut(s)	281, 3125
PsuI	RGATCY	1 cut(s)	2201
PvuII	CAGCTG	3 cut(s)	281, 1988, 3085
RsaI	GTAC	6 cut(s)	211, 484, 752, 1984, 2087, 3110
RsaNI	GTAC	6 cut(s)	210, 483, 751, 1983, 2086, 3109
RseI	CAYNNNNRTG	3 cut(s)	920, 1168, 1685
SacII	CCGCGG	1 cut(s)	2610
SalI	GTCGAC	1 cut(s)	2950
SapI	GCTCTTC	1 cut(s)	2496
Sau3AI	GATC	5 cut(s)	1219, 2201, 2272, 2437, 2875
Sau96I	GGNCC	6 cut(s)	96, 367, 386, 1355, 2601, 3349
SchI	GAGTC	5 cut(s)	316, 1691, 2794, 2957, 3065
ScrFI	CCNGG	9 cut(s)	1209, 1277, 1740, 1866, 2237, 2367, 2376, 2421, 2712
SexAI	ACCWGGT	2 cut(s)	1207, 2374
SfaNI	GCATC	6 cut(s)	405, 1126, 1882, 2055, 2653, 2829
SfcI	CTRYAG	5 cut(s)	810, 981, 1054, 1542, 3474
SfoI	GGCGCC	2 cut(s)	1067, 3145
Sfr303I	CCGCGG	1 cut(s)	2610
SgrBI	CCGCGG	1 cut(s)	2610
SinI	GGWCC	5 cut(s)	96, 367, 386, 1355, 3349
SmiMI	CAYNNNNRTG	3 cut(s)	920, 1168, 1685
SmlI	CTYRAG	3 cut(s)	896, 1421, 2276
SmoI	CTYRAG	3 cut(s)	896, 1421, 2276
SphI	GCATGC	1 cut(s)	184
SspDI	GGCGCC	2 cut(s)	1065, 3143
SspI	AATATT	2 cut(s)	2483, 2890
SspMI	CTAG	3 cut(s)	944, 2598, 3321
StyD4I	CCNGG	9 cut(s)	1207, 1275, 1738, 1864, 2235, 2365, 2374, 2419, 2710
StyI	CCWWGG	6 cut(s)	496, 711, 952, 2955, 3409, 3492
TaaI	ACNGT	8 cut(s)	357, 436, 487, 586, 1746, 2163, 2997, 3349
TaiI	ACGT	2 cut(s)	859, 2701
TaqI	TCGA	6 cut(s)	1332, 1391, 1759, 2440, 2951, 3420
TaqII	GACCGA	1 cut(s)	1372
TatI	WGTACW	3 cut(s)	482, 2085, 3108
TauI	GCSGC	2 cut(s)	2014, 3457
TscAI	CASTG	6 cut(s)	268, 441, 1116, 2143, 2317, 3193
TseFI	GTSAC	8 cut(s)	283, 430, 985, 994, 1111, 1694, 2462, 3460
Tsp45I	GTSAC	8 cut(s)	283, 430, 985, 994, 1111, 1694, 2462, 3460
TspGWI	ACGGA	2 cut(s)	847, 3342
TspRI	CASTG	6 cut(s)	268, 441, 1116, 2143, 2317, 3193
Van91I	CCANNNNNTGG	2 cut(s)	1334, 3125
VpaK11BI	GGWCC	5 cut(s)	96, 367, 386, 1355, 3349
VspI	ATTAAT	1 cut(s)	1779
XagI	CCTNNNNNAGG	4 cut(s)	2138, 2320, 2723, 2774
XapI	RAATTY	7 cut(s)	78, 408, 577, 638, 1916, 3034, 3584
XceI	RCATGY	4 cut(s)	184, 1295, 2587, 3461
XcmI	CCANNNNNNNNNTGG	1 cut(s)	200
XmiI	GTMKAC	1 cut(s)	2951
XmnI	GAANNNNTTC	6 cut(s)	1313, 1416, 1575, 2409, 2641, 3483
XspI	CTAG	3 cut(s)	944, 2598, 3321
Zsp2I	ATGCAT	2 cut(s)	1175, 2668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.