Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003227

Physical Location & Seq

Genomic Coordinates Forward (+)

3968338 .. 3972037

Gene Structure 3700 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G002180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3012 bp

ATGGATAATTTAATGGAAAAGTTTAGTGTAGACGACTCGACGTATGCTCAGGTGCAAGTCCGAGCTACTTTCTATCCCAAGTTTGAGAATGAGAAGTCCGATCAGGAGGTTAGGACAAGGATGATAGAGTTGGTGTCGAAAGGGTTGGCTACATTGGAGGTTTCCCTCAAGCACTCGGGTTCACTTTTTATGTATGCTGGTAGTGAGGGTGGTGCGTATGCGAAAAATAGCTTTGGGAATATATATACTGCTGTGGGTGTGTTTGTTCTGGGGAGGATGTTTCGTGAGGCGTGGGGATCGGAAGCAGCAAGGATGCAAGCAGAGTTTAATGATTTTCTTGAGAGGAACCGTATGTGTATATCAATGGAAATTGTGACTGCTGTTCTAGGAGATCATGGACAGCGTCCGAAAGAAGATTTTGTTGTGGTCACGGCCGTCACGGAGTTGGGCAATGGGAAGCCAAAGTTCTACTCAACTCCCGAGATTATTGCATTCTGTCGGAATTGGCGCCTGCCCACAAATCATGTATGGCTGTTCTCAACAAGGAAAGCAGTGACATCTTTTTTTGCTGCTTTTGATGCCCTGTCTGAAGAAGGAACTGCAACAAGTGTATGCAGAGCTCTTGATGAAGTTGCAGATATATCTATACCAGAGGGTATTGTTGCTCGTATTGTAAGCCATGAGAGCTCAAAGCATATGGAGAAAGTCTTAAAAGATTTCCCTCCTCCACCAATGGAAGGAGCTGGTCTTGATTTGGGACCAAGCCTAAGGGAGATTTGCGCTGCAAATAGGTCTGATGAAACACAGCAAATAAAAGCACTTCTTAAGGGTGTCGGCTCCTCTTTTTGTCCTGACCATTCAGACTGGCTTGGGCCTGGAGCTGGTGATGCACATTCTAAAAATGTAGATCAATCAGTGCTATCAAAATTTTTAAAATCTCATCCTGCTGATTTTTCTACCACCAAATTGCAGGAAGTGGTTTGTTTGATGAGAGAAAAGCGTTTACCTGCTGCCTTTAAATGCTACCAGAACTACCACAAGCATGATTCCATGTCCAGTGACAACGTTTTCTATAAAATGGTCATTCATGTCCGTAGTGACTCTGCATTTCAGCGGTATCAGAAAGAGATGAAGTCAAAGCCAGGACTGTGGCCTTTATATCGAGGTTTTTTCGTCGACATTAATTTGTTCAAGGCAAACAAGGAGAGAGCTGCTGAGATTGCAAAAAACAAAAGCACTGTTGTGGACATAAATGGGGGCTGTGGTATGTCAGGAAAACATGGATTGGCGGAAGAAGATGCAAACTTAATGATCAAATTGAAATTTCTTACATATAAGCTGCGAACTTTTTTGATCCGCAATGGTTTATCAACTCTATTTAAACAAGGTCCGGCTGCTTATAAAGCCTATTACTTGAGGCAAATGAAAATATGGGGCACTTCAGCAGGAAAGCAGAGGGAACTCAGCAAGATGCTTGATGAATGGGCTGTGTACATACGAAGGAAATGTGGAAACAAACACATGTCGTCATCAGTGTACCTCAGCGAGGCTGAGCCTTTCCTTGAACAGTATGCAAAACGTAGCCCACAGAATCAGGCTCTTATAGGATCTGCTGGAAACTTGGTAAATGCTGAAAACTTCTTGGCCATAGTTGAGGGAGGAAGAGATGAAGAGGGTGATCTGGAGAAGGAGTCTGTACCATCAAGCCCCAGTGCCTCAGTTGCAGACACCGTTCCAAAGGCTGAGGGTCTGATTGTATTCTTTCCTGGACTACCTGGTTCTGCCAAGTCAGCACTATGCAAGGAACTACTCAAAGCCCCGGGAGGATTTGGGGATGATCGGCCAGTTCAAAGTCTAATGGGTGATCTTGTTAAAGGAAAATATTGGCAGAAGGTCGCTGATCAGTGCAGGAAAAAACCATATTCTATAATGCTCGCAGACAAGAATGCACCAAACGTAGAAGTGTGGAGACAGGTCGAAGACATGTGCCACAGAACTAGGGCCTCTGCTGTACCAGTTGTTCCTGATTCTGAAGGAACTGAATCAAATCCGTTTTCTCTTGACGCATTAGCGGTTTTCATGTTTCGTGTGCTTCAACGTGCTAATCATCCGGGAAACCTTGACAAAAATTCACCGAATGCTGGCTATGTTCTGCTAATGTTCTATCACCTTTATGAGGGCAAGACTCGGAGAGAATTTGAGTCTGAACTAGTTGAGCGTTTTGGTTCACTTGTCAAAATACCACTGCTGACATCGGAGAGGAATCCATTACCGGATCCTGTGAAATCAACTCTTGAGGATGGAATAAATTTATACAACCTTCACACTGATAGACATGGAAGATGGGATTCAAGCAGGGGAGCCTACACAAAGGAGTGGGCTAAATGGGAGAAACAGTTGCGAGAGGTTTTGTTTGGCAATGCACGGTATTTTAATTCTGTTCAAGTTCCCTTTGAGTCTTCTGTTAAGGAAGTATCAGAACAGCTAAGAAAGATTGCAAAAGGTGAGTATAAGACTCCAGAGTACAGGACTTCAGATTCTGGGAAGAGGAAGATCTCGGCCATTACATTTGCTGCCATCAACCTGCCTATTACCGATATCAAAGGTTTGCTTGATAATTTGGCTGGGAAAGATAGTAAAGTCGAAGCTTTTCTAAAAGACAAGAATTTGGAGAACAGCATTAATAAGGCTCATGTTACTCTTGCGCACAAGAGAAGTCATGGTGTTACTGCTGTGGCTAATTATGGCACCTTCCTACACAAACAGGTCCCTGTGGATATAACTGCACTTCTCTTCACGGATAAAATGGCTGCATTCGAAGCTTGCCCTGGCTCTGTTGAAGGTGAAAGAGTCACCCCCAAAAATGAATGGCCTCATGTTACCTTATGGACAGCAGAGGGTGTTTTAGCCAAGGAGGCCAACTTGTTAAGCAATTTGCATTTAGAGGGCAAGGCTACCCGTATTGCGATTGACCCACCTGCCACCATTCATGGGACGCTGGAGTTTTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000394 GO:0000910 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003972 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006388 GO:0006396 GO:0006399 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008033 GO:0008150 GO:0008152 GO:0008356 GO:0008380 GO:0008452 GO:0009790 GO:0009791 GO:0009793 GO:0009966 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010467 GO:0010646 GO:0010928 GO:0016070 GO:0016874 GO:0016886 GO:0022402 GO:0022414 GO:0023051 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034660 GO:0042245 GO:0043170 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048316 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051301 GO:0061458 GO:0061640 GO:0065007 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

tRNA_lig_CPD

Protein Analysis

1003

Amino Acids

111.91

Weight (kDa)

8.55

Isoelectric Point (pI)

35.28

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07910 AT1G07910
fragaria_vesca	FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_3g33360 FvH4_4g02320
malus_domestica	MD03G1112400.v1.1 MD11G1129800.v1.1
prunus_persica	Prupe.6G094700_v2.0.a1
pyrus_communis	pycom11g10750 pycom11g10760
rosa_chinensis	RchiOBHm_Chr4g0389641 RchiOBHm_Chr5g0060751
rosa_laevigata	RLG00000009964 RLG00000035390
rosa_multiflora	Rmu_co8520825.1_g000001 Rmu_sc0006568.1_g000004 Rmu_sc0007419.1_g000006
rosa_roxburghii	Rroxscaffold_1G00019960 Rroxscaffold_5G00335630
rosa_rugosa	Rorug03G0324600 Rorug05G0333400
rosa_samantha	Rh4AG029200 Rh4BG022400 Rh4CG031200 Rh4DG022900 Rh5AG396400 Rh5BG408600 Rh5CG433200 Rh5DG423800
rosa_wichuraiana	Rw4G002180 Rw5G037370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1401
AarI	CACCTGC	1 cut(s)	2986
Acc16I	TGCGCA	1 cut(s)	2706
Acc36I	ACCTGC	3 cut(s)	1015, 2592, 2986
AccB1I	GGYRCC	2 cut(s)	507, 2747
AccI	GTMKAC	2 cut(s)	30, 1176
AciI	CCGC	4 cut(s)	1114, 1289, 1357, 2072
AclI	AACGTT	1 cut(s)	1065
AclWI	GGATC	5 cut(s)	304, 1348, 1615, 2270, 2283
AcoI	YGGCCR	4 cut(s)	432, 1644, 1841, 2559
AcsI	RAATTY	6 cut(s)	926, 1322, 2128, 2195, 2308, 2665
AcuI	CTGAAG	4 cut(s)	609, 1425, 2052, 2517
AcyI	GRCGYC	1 cut(s)	508
AfaI	GTAC	5 cut(s)	1493, 1538, 1698, 2013, 2525
AfiI	CCNNNNNNNGG	6 cut(s)	737, 881, 1546, 2141, 2176, 2991
AflII	CTTAAG	1 cut(s)	824
AflIII	ACRYGT	2 cut(s)	1521, 1983
AgsI	TTSAA	8 cut(s)	1192, 1321, 1565, 1850, 2096, 2352, 2444, 2840
AhlI	ACTAGT	1 cut(s)	2209
AjnI	CCWGG	5 cut(s)	874, 1141, 1765, 1774, 2827
AleI	CACNNNNGTG	1 cut(s)	1241
Alw21I	GWGCWC	2 cut(s)	622, 689
Alw26I	GTCTC	1 cut(s)	1963
AlwI	GGATC	5 cut(s)	304, 1348, 1615, 2270, 2283
AlwNI	CAGNNNCTG	1 cut(s)	2540
Ama87I	CYCGRG	3 cut(s)	175, 479, 1819
AoxI	GGCC	9 cut(s)	432, 872, 1151, 1644, 1841, 2001, 2559, 2870, 2916
ApeKI	GCWGC	9 cut(s)	305, 569, 782, 1010, 1211, 1339, 1394, 2573, 2810
ApoI	RAATTY	6 cut(s)	926, 1322, 2128, 2195, 2308, 2665
AseI	ATTAAT	2 cut(s)	1182, 2682
Asp700I	GAANNNNTTC	1 cut(s)	2649
AspLEI	GCGC	3 cut(s)	510, 782, 2707
AspS9I	GGNCC	5 cut(s)	758, 872, 1388, 2001, 2767
AsuC2I	CCSGG	3 cut(s)	1820, 1821, 2112
AsuHPI	GGTGA	8 cut(s)	896, 1688, 1874, 2124, 2159, 2516, 2845, 2855
AsuII	TTCGAA	1 cut(s)	2817
AvaI	CYCGRG	3 cut(s)	175, 479, 1819
AvaII	GGWCC	3 cut(s)	758, 1388, 2767
AxyI	CCTNAGG	1 cut(s)	767
BaeGI	GKGCMC	1 cut(s)	1439
BalI	TGGCCA	1 cut(s)	1646
BamHI	GGATCC	1 cut(s)	2275
BanI	GGYRCC	2 cut(s)	507, 2747
BanII	GRGCYC	2 cut(s)	622, 689
BbsI	GAAGAC	2 cut(s)	1986, 2451
Bbv12I	GWGCWC	2 cut(s)	622, 689
BbvCI	CCTCAGC	2 cut(s)	1541, 1743
BbvI	GCAGC	9 cut(s)	317, 556, 769, 997, 1198, 1326, 1381, 2560, 2797
BccI	CCATC	4 cut(s)	1708, 2294, 2337, 2585
BceAI	ACGGC	2 cut(s)	419, 447
BcgI	CGANNNNNNTGC	2 cut(s)	470, 504
BciT130I	CCWGG	5 cut(s)	876, 1143, 1767, 1776, 2829
BclI	TGATCA	2 cut(s)	1311, 1900
BcnI	CCSGG	3 cut(s)	1820, 1821, 2112
BcoDI	GTCTC	1 cut(s)	1963
BcuI	ACTAGT	1 cut(s)	2209
BfaI	CTAG	3 cut(s)	386, 1998, 2210
BfoI	RGCGCY	1 cut(s)	511
BfrI	CTTAAG	1 cut(s)	824
BfuAI	ACCTGC	3 cut(s)	1015, 2592, 2986
BglI	GCCNNNNNGGC	1 cut(s)	2915
BglII	AGATCT	1 cut(s)	2553
BisI	GCNGC	9 cut(s)	306, 570, 783, 1011, 1212, 1340, 1395, 2574, 2811
BlpI	GCTNAGC	1 cut(s)	1551
BlsI	GCNGC	9 cut(s)	307, 571, 784, 1012, 1213, 1341, 1396, 2575, 2812
Bme1390I	CCNGG	8 cut(s)	876, 1143, 1767, 1776, 1820, 1821, 2112, 2829
Bme18I	GGWCC	3 cut(s)	758, 1388, 2767
BmeT110I	CYCGRG	3 cut(s)	175, 479, 1819
BmgT120I	GGNCC	5 cut(s)	758, 872, 1388, 2001, 2767
BmiI	GGNNCC	8 cut(s)	347, 509, 759, 838, 2277, 2362, 2749, 2769
BmrFI	CCNGG	8 cut(s)	876, 1143, 1767, 1776, 1820, 1821, 2112, 2829
BmrI	ACTGGG	1 cut(s)	1704
BmsI	GCATC	5 cut(s)	303, 568, 877, 1288, 1461
BmuI	ACTGGG	1 cut(s)	1704
BpiI	GAAGAC	2 cut(s)	1986, 2451
BpmI	CTGGAG	3 cut(s)	897, 1703, 2502
Bpu10I	CCTNAGC	3 cut(s)	48, 1541, 1743
Bpu1102I	GCTNAGC	1 cut(s)	1551
Bpu14I	TTCGAA	1 cut(s)	2817
BpuEI	CTTGAG	4 cut(s)	152, 359, 1435, 2315
BpuMI	CCSGG	3 cut(s)	1820, 1821, 2112
BsaHI	GRCGYC	1 cut(s)	508
BsaJI	CCNNGG	4 cut(s)	1818, 1819, 2827, 2910
BsaWI	WCCGGW	1 cut(s)	2272
BsaXI	ACNNNNNCTCC	4 cut(s)	1676, 1706, 2381, 2411
Bsc4I	CCNNNNNNNGG	6 cut(s)	737, 881, 1546, 2141, 2176, 2991
Bse1I	ACTGG	5 cut(s)	869, 1056, 1710, 1844, 2015
Bse21I	CCTNAGG	1 cut(s)	767
Bse3DI	GCAATG	3 cut(s)	457, 1366, 2425
BseBI	CCWGG	5 cut(s)	876, 1143, 1767, 1776, 2829
BseDI	CCNNGG	4 cut(s)	1818, 1819, 2827, 2910
BseGI	GGATG	7 cut(s)	126, 282, 318, 940, 1840, 2107, 2305
BseLI	CCNNNNNNNGG	6 cut(s)	737, 881, 1546, 2141, 2176, 2991
BseMI	GCAATG	3 cut(s)	457, 1366, 2425
BseMII	CTCAG	7 cut(s)	62, 1206, 1477, 1542, 1555, 1731, 1734
BseNI	ACTGG	5 cut(s)	869, 1056, 1710, 1844, 2015
BseRI	GAGGAG	2 cut(s)	714, 829
BseSI	GKGCMC	1 cut(s)	1439
BseX3I	CGGCCG	1 cut(s)	432
BseXI	GCAGC	9 cut(s)	317, 556, 769, 997, 1198, 1326, 1381, 2560, 2797
BseYI	CCCAGC	1 cut(s)	2624
BsgI	GTGCAG	2 cut(s)	1927, 2769
Bsh1285I	CGRYCG	1 cut(s)	435
BshFI	GGCC	9 cut(s)	434, 874, 1153, 1646, 1843, 2003, 2561, 2872, 2918
BshNI	GGYRCC	2 cut(s)	507, 2747
BsiEI	CGRYCG	1 cut(s)	435
BsiHKAI	GWGCWC	2 cut(s)	622, 689
BsiHKCI	CYCGRG	3 cut(s)	175, 479, 1819
BsiSI	CCGG	4 cut(s)	1391, 1820, 2111, 2273
BslFI	GGGAC	3 cut(s)	771, 2753, 3007
BslI	CCNNNNNNNGG	6 cut(s)	737, 881, 1546, 2141, 2176, 2991
BsmAI	GTCTC	1 cut(s)	1963
BsmFI	GGGAC	3 cut(s)	771, 2753, 3007
BsmI	GAATGC	4 cut(s)	491, 1951, 2143, 2813
BsnI	GGCC	9 cut(s)	434, 874, 1153, 1646, 1843, 2003, 2561, 2872, 2918
BsoBI	CYCGRG	3 cut(s)	175, 479, 1819
Bsp119I	TTCGAA	1 cut(s)	2817
Bsp1286I	GDGCHC	3 cut(s)	622, 689, 1439
Bsp1407I	TGTACA	1 cut(s)	1491
Bsp1720I	GCTNAGC	1 cut(s)	1551
BspACI	CCGC	4 cut(s)	1114, 1289, 1357, 2072
BspANI	GGCC	9 cut(s)	434, 874, 1153, 1646, 1843, 2003, 2561, 2872, 2918
BspCNI	CTCAG	7 cut(s)	61, 1207, 1476, 1543, 1554, 1730, 1735
BspLI	GGNNCC	8 cut(s)	347, 509, 759, 838, 2277, 2362, 2749, 2769
BspMI	ACCTGC	3 cut(s)	1015, 2592, 2986
BspPI	GGATC	5 cut(s)	304, 1348, 1615, 2270, 2283
BspT104I	TTCGAA	1 cut(s)	2817
BspT107I	GGYRCC	2 cut(s)	507, 2747
BspTI	CTTAAG	1 cut(s)	824
BsrDI	GCAATG	3 cut(s)	457, 1366, 2425
BsrGI	TGTACA	1 cut(s)	1491
BsrI	ACTGG	5 cut(s)	869, 1056, 1710, 1844, 2015
BssECI	CCNNGG	4 cut(s)	1818, 1819, 2827, 2910
BssNI	GRCGYC	1 cut(s)	508
BssT1I	CCWWGG	1 cut(s)	2910
Bst2UI	CCWGG	5 cut(s)	876, 1143, 1767, 1776, 2829
Bst4CI	ACNGT	7 cut(s)	350, 1149, 1240, 1569, 1732, 2397, 2427
Bst6I	CTCTTC	4 cut(s)	1657, 1665, 2540, 2798
BstACI	GRCGYC	1 cut(s)	508
BstAFI	CTTAAG	1 cut(s)	824
BstAUI	TGTACA	1 cut(s)	1491
BstBI	TTCGAA	1 cut(s)	2817
BstC8I	GCNNGC	5 cut(s)	318, 512, 1935, 2143, 2824
BstDEI	CTNAG	9 cut(s)	48, 767, 1215, 1463, 1541, 1551, 1717, 1743, 2486
BstENI	CCTNNNNNAGG	2 cut(s)	1544, 2174
BstF5I	GGATG	7 cut(s)	126, 282, 318, 940, 1840, 2107, 2305
BstH2I	RGCGCY	1 cut(s)	511
BstHHI	GCGC	3 cut(s)	510, 782, 2707
BstMAI	GTCTC	1 cut(s)	1963
BstMCI	CGRYCG	1 cut(s)	435
BstMWI	GCNNNNNNNGC	9 cut(s)	578, 684, 887, 1220, 1403, 1721, 1790, 2819, 2915
BstNI	CCWGG	5 cut(s)	876, 1143, 1767, 1776, 2829
BstNSI	RCATGY	2 cut(s)	1525, 1987
BstSCI	CCNGG	8 cut(s)	874, 1141, 1765, 1774, 1818, 1819, 2110, 2827
BstSLI	GKGCMC	1 cut(s)	1439
BstV1I	GCAGC	9 cut(s)	317, 556, 769, 997, 1198, 1326, 1381, 2560, 2797
BstV2I	GAAGAC	2 cut(s)	1986, 2451
BstX2I	RGATCY	3 cut(s)	1607, 2275, 2553
BstXI	CCANNNNNNTGG	1 cut(s)	1149
BstYI	RGATCY	3 cut(s)	1607, 2275, 2553
BstZI	CGGCCG	1 cut(s)	432
Bsu36I	CCTNAGG	1 cut(s)	767
BsuRI	GGCC	9 cut(s)	434, 874, 1153, 1646, 1843, 2003, 2561, 2872, 2918
BtsCI	GGATG	7 cut(s)	126, 282, 318, 940, 1840, 2107, 2305
BtsI	GCAGTG	2 cut(s)	558, 2243
BtsIMutI	CAGTG	9 cut(s)	558, 921, 1063, 1236, 1539, 1717, 1910, 2243, 2325
BveI	ACCTGC	3 cut(s)	1015, 2592, 2986
Cac8I	GCNNGC	5 cut(s)	318, 512, 1935, 2143, 2824
CaiI	CAGNNNCTG	1 cut(s)	2540
CfoI	GCGC	3 cut(s)	510, 782, 2707
Cfr13I	GGNCC	5 cut(s)	758, 872, 1388, 2001, 2767
Cfr9I	CCCGGG	1 cut(s)	1819
CseI	GACGC	3 cut(s)	392, 2072, 3004
CsiI	ACCWGGT	1 cut(s)	1774
Csp6I	GTAC	5 cut(s)	1492, 1537, 1697, 2012, 2524
CviQI	GTAC	5 cut(s)	1492, 1537, 1697, 2012, 2524
DdeI	CTNAG	9 cut(s)	48, 767, 1215, 1463, 1541, 1551, 1717, 1743, 2486
DinI	GGCGCC	1 cut(s)	509
DraI	TTTAAA	3 cut(s)	933, 1018, 1381
EaeI	YGGCCR	4 cut(s)	432, 1644, 1841, 2559
EagI	CGGCCG	1 cut(s)	432
Eam1104I	CTCTTC	4 cut(s)	1657, 1665, 2540, 2798
EarI	CTCTTC	4 cut(s)	1657, 1665, 2540, 2798
EciI	GGCGGA	1 cut(s)	1304
Ecl136II	GAGCTC	2 cut(s)	620, 687
EclXI	CGGCCG	1 cut(s)	432
Eco130I	CCWWGG	1 cut(s)	2910
Eco24I	GRGCYC	2 cut(s)	622, 689
Eco32I	GATATC	1 cut(s)	2599
Eco47I	GGWCC	3 cut(s)	758, 1388, 2767
Eco52I	CGGCCG	1 cut(s)	432
Eco53kI	GAGCTC	2 cut(s)	620, 687
Eco57I	CTGAAG	4 cut(s)	609, 1425, 2052, 2517
Eco81I	CCTNAGG	1 cut(s)	767
Eco88I	CYCGRG	3 cut(s)	175, 479, 1819
EcoICRI	GAGCTC	2 cut(s)	620, 687
EcoNI	CCTNNNNNAGG	2 cut(s)	1544, 2174
EcoO109I	RGGNCCY	2 cut(s)	2001, 2767
EcoRII	CCWGG	5 cut(s)	874, 1141, 1765, 1774, 2827
EcoRV	GATATC	1 cut(s)	2599
EcoT14I	CCWWGG	1 cut(s)	2910
EcoT38I	GRGCYC	2 cut(s)	622, 689
EgeI	GGCGCC	1 cut(s)	509
EheI	GGCGCC	1 cut(s)	509
ErhI	CCWWGG	1 cut(s)	2910
FalI	AAGNNNNNCTT	4 cut(s)	807, 839, 2595, 2627
FaqI	GGGAC	3 cut(s)	771, 2753, 3007
FauNDI	CATATG	1 cut(s)	696
FbaI	TGATCA	2 cut(s)	1311, 1900
FblI	GTMKAC	2 cut(s)	30, 1176
Fnu4HI	GCNGC	9 cut(s)	306, 570, 783, 1011, 1212, 1340, 1395, 2574, 2811
FokI	GGATG	7 cut(s)	133, 289, 325, 927, 1847, 2094, 2312
FriOI	GRGCYC	2 cut(s)	622, 689
Fsp4HI	GCNGC	9 cut(s)	306, 570, 783, 1011, 1212, 1340, 1395, 2574, 2811
FspBI	CTAG	3 cut(s)	386, 1998, 2210
FspI	TGCGCA	1 cut(s)	2706
GlaI	GCGC	3 cut(s)	509, 781, 2706
GluI	GCNGC	9 cut(s)	306, 570, 783, 1011, 1212, 1340, 1395, 2574, 2811
GsaI	CCCAGC	1 cut(s)	2628
GsuI	CTGGAG	3 cut(s)	897, 1703, 2502
HaeII	RGCGCY	1 cut(s)	511
HaeIII	GGCC	9 cut(s)	434, 874, 1153, 1646, 1843, 2003, 2561, 2872, 2918
HapII	CCGG	4 cut(s)	1391, 1820, 2111, 2273
HgaI	GACGC	3 cut(s)	392, 2072, 3004
HhaI	GCGC	3 cut(s)	510, 782, 2707
Hin1I	GRCGYC	1 cut(s)	508
Hin6I	GCGC	3 cut(s)	508, 780, 2705
HinP1I	GCGC	3 cut(s)	508, 780, 2705
HincII	GTYRAC	1 cut(s)	1177
HindII	GTYRAC	1 cut(s)	1177
HindIII	AAGCTT	2 cut(s)	2646, 2820
HpaII	CCGG	4 cut(s)	1391, 1820, 2111, 2273
HphI	GGTGA	8 cut(s)	896, 1688, 1874, 2124, 2159, 2516, 2845, 2855
Hpy166II	GTNNAC	8 cut(s)	31, 182, 1004, 1177, 1246, 1492, 1537, 2228
Hpy8I	GTNNAC	8 cut(s)	31, 182, 1004, 1177, 1246, 1492, 1537, 2228
Hpy99I	CGWCG	2 cut(s)	43, 1178
HpyAV	CCTTC	9 cut(s)	587, 731, 1494, 1681, 1885, 2027, 2330, 2761, 2834
HpyCH4III	ACNGT	7 cut(s)	350, 1149, 1240, 1569, 1732, 2397, 2427
HpyCH4IV	ACGT	5 cut(s)	41, 1065, 1579, 1956, 2098
HpyF10VI	GCNNNNNNNGC	9 cut(s)	578, 684, 887, 1220, 1403, 1721, 1790, 2819, 2915
HpyF3I	CTNAG	9 cut(s)	48, 767, 1215, 1463, 1541, 1551, 1717, 1743, 2486
HpySE526I	ACGT	5 cut(s)	41, 1065, 1579, 1956, 2098
Hsp92I	GRCGYC	1 cut(s)	508
HspAI	GCGC	3 cut(s)	508, 780, 2705
KasI	GGCGCC	1 cut(s)	507
Ksp22I	TGATCA	2 cut(s)	1311, 1900
LmnI	GCTCC	4 cut(s)	740, 842, 878, 2360
Lsp1109I	GCAGC	9 cut(s)	317, 556, 769, 997, 1198, 1326, 1381, 2560, 2797
LweI	GCATC	5 cut(s)	303, 568, 877, 1288, 1461
MabI	ACCWGGT	1 cut(s)	1774
MaeI	CTAG	3 cut(s)	386, 1998, 2210
MaeII	ACGT	5 cut(s)	41, 1065, 1579, 1956, 2098
MflI	RGATCY	3 cut(s)	1607, 2275, 2553
MhlI	GDGCHC	3 cut(s)	622, 689, 1439
MlsI	TGGCCA	1 cut(s)	1646
MluNI	TGGCCA	1 cut(s)	1646
Mly113I	GGCGCC	1 cut(s)	508
MlyI	GAGTC	8 cut(s)	29, 1094, 1700, 2179, 2210, 2465, 2509, 2859
MmeI	TCCRAC	1 cut(s)	479
Mox20I	TGGCCA	1 cut(s)	1646
MroXI	GAANNNNTTC	1 cut(s)	2649
MscI	TGGCCA	1 cut(s)	1646
MslI	CAYNNNNRTG	3 cut(s)	1041, 1241, 2172
Msp20I	TGGCCA	1 cut(s)	1646
MspA1I	CMGCKG	1 cut(s)	1114
MspCI	CTTAAG	1 cut(s)	824
MspI	CCGG	4 cut(s)	1391, 1820, 2111, 2273
MspR9I	CCNGG	8 cut(s)	876, 1143, 1767, 1776, 1820, 1821, 2112, 2829
Mva1269I	GAATGC	4 cut(s)	491, 1951, 2143, 2813
MvaI	CCWGG	5 cut(s)	876, 1143, 1767, 1776, 2829
MwoI	GCNNNNNNNGC	9 cut(s)	578, 684, 887, 1220, 1403, 1721, 1790, 2819, 2915
NarI	GGCGCC	1 cut(s)	508
NciI	CCSGG	3 cut(s)	1820, 1821, 2112
NdeI	CATATG	1 cut(s)	696
NlaIV	GGNNCC	8 cut(s)	347, 509, 759, 838, 2277, 2362, 2749, 2769
NmeAIII	GCCGAG	1 cut(s)	2537
NmuCI	GTSAC	7 cut(s)	373, 427, 436, 553, 1058, 1097, 2851
NsbI	TGCGCA	1 cut(s)	2706
NspI	RCATGY	2 cut(s)	1525, 1987
NspV	TTCGAA	1 cut(s)	2817
OliI	CACNNNNGTG	1 cut(s)	1241
PaqCI	CACCTGC	1 cut(s)	2986
PciI	ACATGT	2 cut(s)	1521, 1983
PctI	GAATGC	4 cut(s)	491, 1951, 2143, 2813
PdmI	GAANNNNTTC	1 cut(s)	2649
PfeI	GAWTC	7 cut(s)	1046, 1591, 2027, 2042, 2263, 2348, 2537
PflFI	GACNNNGTC	1 cut(s)	402
PfoI	TCCNGGA	2 cut(s)	1765, 2110
PkrI	GCNGC	9 cut(s)	307, 571, 784, 1012, 1213, 1341, 1396, 2575, 2812
PleI	GAGTC	8 cut(s)	29, 1094, 1699, 2179, 2209, 2464, 2509, 2858
PluTI	GGCGCC	1 cut(s)	511
PpsI	GAGTC	8 cut(s)	29, 1094, 1699, 2179, 2209, 2464, 2509, 2858
PpuMI	RGGWCCY	1 cut(s)	2767
PscI	ACATGT	2 cut(s)	1521, 1983
PshBI	ATTAAT	2 cut(s)	1182, 2682
PsiI	TTATAA	1 cut(s)	1401
Psp124BI	GAGCTC	2 cut(s)	622, 689
Psp1406I	AACGTT	1 cut(s)	1065
Psp5II	RGGWCCY	1 cut(s)	2767
Psp6I	CCWGG	5 cut(s)	874, 1141, 1765, 1774, 2827
PspFI	CCCAGC	1 cut(s)	2624
PspGI	CCWGG	5 cut(s)	874, 1141, 1765, 1774, 2827
PspN4I	GGNNCC	8 cut(s)	347, 509, 759, 838, 2277, 2362, 2749, 2769
PspPI	GGNCC	5 cut(s)	758, 872, 1388, 2001, 2767
PspPPI	RGGWCCY	1 cut(s)	2767
PsrI	GAACNNNNNNTAC	2 cut(s)	2004, 2036
PstNI	CAGNNNCTG	1 cut(s)	2540
PsuI	RGATCY	3 cut(s)	1607, 2275, 2553
PsyI	GACNNNGTC	1 cut(s)	402
RsaI	GTAC	5 cut(s)	1493, 1538, 1698, 2013, 2525
RsaNI	GTAC	5 cut(s)	1492, 1537, 1697, 2012, 2524
RseI	CAYNNNNRTG	3 cut(s)	1041, 1241, 2172
SacI	GAGCTC	2 cut(s)	622, 689
SalI	GTCGAC	1 cut(s)	1175
SatI	GCNGC	9 cut(s)	306, 570, 783, 1011, 1212, 1340, 1395, 2574, 2811
Sau96I	GGNCC	5 cut(s)	758, 872, 1388, 2001, 2767
SchI	GAGTC	8 cut(s)	29, 1094, 1700, 2179, 2210, 2465, 2509, 2859
ScrFI	CCNGG	8 cut(s)	876, 1143, 1767, 1776, 1820, 1821, 2112, 2829
SduI	GDGCHC	3 cut(s)	622, 689, 1439
SexAI	ACCWGGT	1 cut(s)	1774
SfaNI	GCATC	5 cut(s)	303, 568, 877, 1288, 1461
SfoI	GGCGCC	1 cut(s)	509
SfuI	TTCGAA	1 cut(s)	2817
SinI	GGWCC	3 cut(s)	758, 1388, 2767
SmaI	CCCGGG	1 cut(s)	1821
SmiMI	CAYNNNNRTG	3 cut(s)	1041, 1241, 2172
SmlI	CTYRAG	5 cut(s)	167, 338, 824, 1414, 2294
SmoI	CTYRAG	5 cut(s)	167, 338, 824, 1414, 2294
SpeI	ACTAGT	1 cut(s)	2209
SsiI	CCGC	4 cut(s)	1114, 1289, 1357, 2072
SspDI	GGCGCC	1 cut(s)	507
SspI	AATATT	1 cut(s)	1883
SspMI	CTAG	3 cut(s)	386, 1998, 2210
SstI	GAGCTC	2 cut(s)	622, 689
StyD4I	CCNGG	8 cut(s)	874, 1141, 1765, 1774, 1818, 1819, 2110, 2827
StyI	CCWWGG	1 cut(s)	2910
TaaI	ACNGT	7 cut(s)	350, 1149, 1240, 1569, 1732, 2397, 2427
TaiI	ACGT	5 cut(s)	44, 1068, 1582, 1959, 2101
TaqI	TCGA	7 cut(s)	38, 137, 1162, 1176, 1977, 2643, 2817
TatI	WGTACW	2 cut(s)	1491, 2523
TfiI	GAWTC	7 cut(s)	1046, 1591, 2027, 2042, 2263, 2348, 2537
TscAI	CASTG	9 cut(s)	558, 921, 1063, 1243, 1539, 1717, 1910, 2250, 2332
TseFI	GTSAC	7 cut(s)	373, 427, 436, 553, 1058, 1097, 2851
TseI	GCWGC	9 cut(s)	305, 569, 782, 1010, 1211, 1339, 1394, 2573, 2810
Tsp45I	GTSAC	7 cut(s)	373, 427, 436, 553, 1058, 1097, 2851
TspGWI	ACGGA	4 cut(s)	455, 1082, 2040, 2813
TspMI	CCCGGG	1 cut(s)	1819
TspRI	CASTG	9 cut(s)	558, 921, 1063, 1243, 1539, 1717, 1910, 2250, 2332
Tth111I	GACNNNGTC	1 cut(s)	402
Vha464I	CTTAAG	1 cut(s)	824
VpaK11BI	GGWCC	3 cut(s)	758, 1388, 2767
VspI	ATTAAT	2 cut(s)	1182, 2682
XagI	CCTNNNNNAGG	2 cut(s)	1544, 2174
XapI	RAATTY	6 cut(s)	926, 1322, 2128, 2195, 2308, 2665
XceI	RCATGY	2 cut(s)	1525, 1987
XmaI	CCCGGG	1 cut(s)	1819
XmiI	GTMKAC	2 cut(s)	30, 1176
XmnI	GAANNNNTTC	1 cut(s)	2649
XspI	CTAG	3 cut(s)	386, 1998, 2210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.