MD11G1144600.v1.1

Histone H2A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003260
Physical Location & Seq
Forward (+)
13415429 .. 13416739
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1144600.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGAGACTGGTGGTAAGTTGAAGAAAGGTGCCGGGGGAAGGAAGGGCGGTGGTCCGAAGAAGAAGGCGGTGACCCGGTCCGTCAGAGCCGGCCTCCAGTTCCCAGTCGGAAGAATCGGTCGCTACTTGAAGAAGGGGAGGTATGCTCAGAGAGTCGGGTCTGGAGCTCCGGTTTACTTAGCCGCTGTGCTCGAGTACCTCGCAGCTGAAGTGCTGGAATTGGCTGGAAATGCAGCTCGGGACAACAAGAAGAACAGGATTATCCCAAGGCATCTGCTACTTGCTGTGAGGAACGATGAGGAGCTTGGAAAATTGCTTGCTGGTGTGACCATTGCTCACGGCGGCGTGCTTCCAAACATTAACCCAGTCCTCCTGCCCAAGAAATCAGAAAAGGCCGCCGGAAAAGAGCCAAAATCGCCATCGAAGGCTACCAAGTCTCCCAAGAAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04217 ko05034 ko05322 map04217 map05034 map05322
Pfam Domains
Protein Families
Histone Histone_H2A_C

Protein Analysis

151

Amino Acids

15.81

Weight (kDa)

10.66

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 20 - 97 2.3e-12 Core histone H2A/H2B/H3/H4 domain
Histone_H2A_C PF16211 100 - 133 1.1e-18 C-terminus of histone H2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 29
AciI CCGC 5 cut(s) 48, 68, 183, 342, 396
AcuI CTGAAG 1 cut(s) 228
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 1 cut(s) 39
AgsI TTSAA 2 cut(s) 22, 130
AluBI AGCT 4 cut(s) 167, 206, 236, 304
AluI AGCT 4 cut(s) 167, 206, 236, 304
Alw21I GWGCWC 2 cut(s) 169, 192
Alw26I GTCTC 1 cut(s) 441
Ama87I CYCGRG 2 cut(s) 191, 237
AoxI GGCC 2 cut(s) 91, 393
ApeKI GCWGC 2 cut(s) 203, 233
AspS9I GGNCC 2 cut(s) 53, 78
AsuC2I CCSGG 2 cut(s) 34, 76
AsuHPI GGTGA 1 cut(s) 82
AvaI CYCGRG 2 cut(s) 191, 237
AvaII GGWCC 2 cut(s) 53, 78
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 169
Bbv12I GWGCWC 2 cut(s) 169, 192
BbvI GCAGC 2 cut(s) 215, 245
BccI CCATC 1 cut(s) 427
BceAI ACGGC 1 cut(s) 355
BcnI CCSGG 2 cut(s) 34, 76
BcoDI GTCTC 1 cut(s) 441
BisI GCNGC 5 cut(s) 183, 204, 234, 343, 396
BlsI GCNGC 5 cut(s) 184, 205, 235, 344, 397
Bme1390I CCNGG 2 cut(s) 34, 76
Bme18I GGWCC 2 cut(s) 53, 78
BmeT110I CYCGRG 2 cut(s) 191, 237
BmgT120I GGNCC 2 cut(s) 53, 78
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 2 cut(s) 34, 76
BmrI ACTGGG 2 cut(s) 98, 359
BmsI GCATC 1 cut(s) 280
BmuI ACTGGG 2 cut(s) 98, 359
BplI GAGNNNNNCTC 4 cut(s) 78, 110, 130, 162
BpmI CTGGAG 2 cut(s) 80, 183
BpuMI CCSGG 2 cut(s) 34, 76
BsaJI CCNNGG 2 cut(s) 33, 266
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 4 cut(s) 156, 186, 421, 451
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 4 cut(s) 13, 97, 104, 365
Bse3DI GCAATG 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 33, 266
BseLI CCNNNNNNNGG 1 cut(s) 39
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 4 cut(s) 13, 97, 104, 365
BseRI GAGGAG 1 cut(s) 314
BseXI GCAGC 2 cut(s) 215, 245
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 2 cut(s) 93, 395
BshNI GGYRCC 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 2 cut(s) 169, 192
BsiHKCI CYCGRG 2 cut(s) 191, 237
BsiSI CCGG 5 cut(s) 33, 76, 90, 170, 399
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 39
BsmAI GTCTC 1 cut(s) 441
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 2 cut(s) 93, 395
BsoBI CYCGRG 2 cut(s) 191, 237
Bsp1286I GDGCHC 2 cut(s) 169, 192
BspACI CCGC 5 cut(s) 48, 68, 183, 342, 396
BspANI GGCC 2 cut(s) 93, 395
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 1 cut(s) 31
BspT107I GGYRCC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 4 cut(s) 13, 97, 104, 365
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 33, 266
BssT1I CCWWGG 1 cut(s) 266
BstC8I GCNNGC 3 cut(s) 91, 318, 347
BstDEI CTNAG 3 cut(s) 147, 178, 450
BstEII GGTNACC 1 cut(s) 70
BstMAI GTCTC 1 cut(s) 441
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 2 cut(s) 230, 415
BstPI GGTNACC 1 cut(s) 70
BstSCI CCNGG 2 cut(s) 32, 74
BstV1I GCAGC 2 cut(s) 215, 245
BsuRI GGCC 2 cut(s) 93, 395
Cac8I GCNNGC 3 cut(s) 91, 318, 347
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 2 cut(s) 53, 78
CpoI CGGWCCG 1 cut(s) 78
Csp6I GTAC 1 cut(s) 196
CspI CGGWCCG 1 cut(s) 78
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 3 cut(s) 147, 178, 450
Ecl136II GAGCTC 1 cut(s) 167
Eco130I CCWWGG 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 169
Eco47I GGWCC 2 cut(s) 53, 78
Eco53kI GAGCTC 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 228
Eco88I CYCGRG 2 cut(s) 191, 237
Eco91I GGTNACC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 167
EcoO65I GGTNACC 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 266
EcoT38I GRGCYC 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 266
FaiI YATR 1 cut(s) 144
FaqI GGGAC 1 cut(s) 254
Fnu4HI GCNGC 5 cut(s) 183, 204, 234, 343, 396
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 5 cut(s) 183, 204, 234, 343, 396
GluI GCNGC 5 cut(s) 183, 204, 234, 343, 396
GsuI CTGGAG 2 cut(s) 80, 183
HaeIII GGCC 2 cut(s) 93, 395
HapII CCGG 5 cut(s) 33, 76, 90, 170, 399
HinfI GANTC 2 cut(s) 114, 153
HpaII CCGG 5 cut(s) 33, 76, 90, 170, 399
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 5 cut(s) 57, 86, 110, 150, 388
Hpy188III TCNNGA 2 cut(s) 162, 239
Hpy8I GTNNAC 1 cut(s) 175
HpyAV CCTTC 6 cut(s) 33, 37, 58, 127, 418, 439
HpyCH4V TGCA 1 cut(s) 233
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 415
HpyF3I CTNAG 3 cut(s) 147, 178, 450
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
LmnI GCTCC 3 cut(s) 164, 172, 301
Lsp1109I GCAGC 2 cut(s) 215, 245
LweI GCATC 1 cut(s) 280
MaeIII GTNAC 2 cut(s) 70, 325
MboII GAAGA 6 cut(s) 34, 70, 73, 123, 142, 262
MhlI GDGCHC 2 cut(s) 169, 192
MluCI AATT 2 cut(s) 218, 311
MlyI GAGTC 1 cut(s) 162
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 6 cut(s) 104, 132, 209, 282, 292, 380
MroNI GCCGGC 1 cut(s) 89
MseI TTAA 1 cut(s) 360
MspA1I CMGCKG 2 cut(s) 185, 206
MspI CCGG 5 cut(s) 33, 76, 90, 170, 399
MspR9I CCNGG 2 cut(s) 34, 76
MwoI GCNNNNNNNGC 2 cut(s) 230, 415
NaeI GCCGGC 1 cut(s) 91
NciI CCSGG 2 cut(s) 34, 76
NgoMIV GCCGGC 1 cut(s) 89
NlaIV GGNNCC 1 cut(s) 31
NmuCI GTSAC 2 cut(s) 70, 325
PaeR7I CTCGAG 1 cut(s) 191
PdiI GCCGGC 1 cut(s) 91
PfeI GAWTC 1 cut(s) 114
PflFI GACNNNGTC 1 cut(s) 76
PkrI GCNGC 5 cut(s) 184, 205, 235, 344, 397
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 169
PspEI GGTNACC 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 2 cut(s) 53, 78
PspXI VCTCGAGB 1 cut(s) 191
PsyI GACNNNGTC 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
Rsr2I CGGWCCG 1 cut(s) 78
RsrII CGGWCCG 1 cut(s) 78
SacI GAGCTC 1 cut(s) 169
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 5 cut(s) 183, 204, 234, 343, 396
Sau96I GGNCC 2 cut(s) 53, 78
SchI GAGTC 1 cut(s) 162
ScrFI CCNGG 2 cut(s) 34, 76
SduI GDGCHC 2 cut(s) 169, 192
SetI ASST 7 cut(s) 31, 143, 169, 201, 208, 238, 306
SfaNI GCATC 1 cut(s) 280
Sfr274I CTCGAG 1 cut(s) 191
SinI GGWCC 2 cut(s) 53, 78
SlaI CTCGAG 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 2 cut(s) 218, 311
SsiI CCGC 5 cut(s) 48, 68, 183, 342, 396
SstI GAGCTC 1 cut(s) 169
StyD4I CCNGG 2 cut(s) 32, 74
StyI CCWWGG 1 cut(s) 266
TaqI TCGA 2 cut(s) 192, 422
TaqII GACCGA 1 cut(s) 107
TasI AATT 2 cut(s) 218, 311
TauI GCSGC 3 cut(s) 185, 345, 398
TfiI GAWTC 1 cut(s) 114
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TseFI GTSAC 2 cut(s) 70, 325
TseI GCWGC 2 cut(s) 203, 233
Tsp45I GTSAC 2 cut(s) 70, 325
TspGWI ACGGA 1 cut(s) 70
Tth111I GACNNNGTC 1 cut(s) 76
VpaK11BI GGWCC 2 cut(s) 53, 78
XhoI CTCGAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.