Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0007030

Physical Location & Seq

Genomic Coordinates Forward (+)

25576544 .. 25585530

Gene Structure 8987 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1186800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3327 bp

ATGTCGCGGACCGATAGAATGGGTTCGGATCTTAGCAGGACCGGCCCCGTCGAAAGAGACATCGAGCAGGCTATTACTGCTCTAAAGAAAGGGGCATACTTGCTCAAGTATGGAAGAAGGGGGAAGCCTAAGTTTTGTCCATTTCGTCTTTCCAATGATGAATCCGTTCTAATCTGGTTCTCTGGGAAAGAGGAAAAGCACCTTAAGCTAAACCACGTGTCTAGAATTATTTCTGGACAAAGGACTCCAATCTTTCAAAGGTATCCACGGCCTGAGAAGGAGTACCAGTCATTTTCTTTAATATATAATGAGAGGTCACTAGATTTGATTTGCAAAGATAAAGATGAAGCTGAGGTTTGGTTTAGTGGCTTAAAAGCATTGATATCCCGCAGCCATCATCGAAAATGGAGAACAGAGTCAAGGAGTGATGGAATTCCATCTGAAGCAAATAGTCCTAGAACATACACGCGGAGAAGCTCTCCTCTAAATTCTCCATTTGGTAGTAATGATAGCTTACAGAAGGATAGTGCAGATCACCTTCGCCTTCGTAGTCCGTATGAAAGTCCTCCTAAAAATGGTCTGGATAAGGCACTTTCAGATGTGATACTGTATGCTGTTCCTCCCAAGGGTTTCTTCCCTTCAGATTCGGCCAGTGCTTCCGTACATTCTGTGTCATCTGGGGGCTCAGACAGTGTACATGGGCAGATGAAGGCAATGGCAATGGATGCTGTTAGAGTTAGTCTATCCAGTGCTGTTAGCTCATCAAGCCAAGGTTCTGGTCATGATGATGGTGATGCGTTGGGAGATGTTTTCATGTGGGGAGAAGGCACAGGAGATGGTGTTGTGGGTGGTGGTTCTCATAGAGTTGGAAGCTGTCTTGGTGCAAAAATGGATTCTTTGTTGCCCAAAGCCTTAGAATCTAAAGTTGTACTTGACGTCCAAAACATTGCCTGTGGTGGTAGGCATGCTGCTTTAGTAACTAAGCAAGGGGAGATTTTCTCTTGGGGAGAGGAATCGGGGGGCAGGCTTGGGCATGGTGTGGATGTTGACGTTTTGCAACCAAAGCTCATTGATGCACTTAGCAATATCAACATTGAGCTTGTTGCTTGTGGTGAGTACCATACTTGTGCTGTTACGCTTTCTGGTGATCTGTACACATGGGGTGATGGAACTTACAATTTCGGTCTACTTGGACATGGAAATGAAGTGAGCCATTGGGTCCCCAAAAGAGTAAATGGACCCTTGGAGGCTATGCATGTCTCATCCATATCTTGTGGACCCTGGCACACAGCCGTAGTAACTTCTGCTGGGCAGTTGTTTACTTTTGGTGATGGTACATTTGGTGTTCTTGGCCATGGTGATCGGAAAAGTGTTTCAATTCCCAGGGAAGTGGAATCCCTGAAGGGCCTTCGCACTGTGCGAGCAGCTTGTGGTGCTTGGCATACTGCTGCAGTTGTAGAAGTCATGGTTGGGAATTCAAGTGCCAGCAACTGTTCATTAGGAAAGCTGTTCACTTGGGGAGATGGTGATAAGGGTAGGCTTGGGCATGGTGACAAGGAAGCAAAACTTGTGCCTACCTGTGTTGCCGCTCTTGTTGAATCCAATTTTTGTCGAGTTGCTTGTGGACATAGTATGACAGTTGCTCGTACAACATCTGGTCACGTCTACACAATGGGAAGTCCTGTTTATGGCCAGTTAGGAAATCCTCAAGCTGATGGAAAGCTTCCAACTCGAGTTGAGGGAAAGCTCTCCAAGAGTTTTGTGGAAGAGATTGCTTGTGGTGCCTATCATGTTGCAGTTTTAACTTCAAGAACTGAAGTGTATACTTGGGGAAAAGGAGCAAATGGTCGTTTAGGTCATGGGAATATAGATGATCGGAATTCCCCAACATTAGTTGAAGCTCTGAAAGATAAGCAAGTCAAAAGTATTGCTTGCGGTGCTAATTTTACTGCAGTTATATGCCTTCATAAATGGGTTTCAGGAGTTGATCAGTCAATGTGTTCGGGCTGCCGGGTTCCATTTAATTTTAAAAGAAAACGCCATAATTGTTATAATTGTGGGCTTGTGTTTTGTCATTCTTGCAGCAGTAAGAAATCACTCAAAGCTTCCATGGCACCAAATCCTAGCAAACCTTATCGTGTCTGTGATAATTGTTTTAATAAACTGAGAAAAGCTGCTGAAACTGATACTTCATTACCAACATCTATAAGCAGAAGAGGAAGTATCAATCAGGGTTCAAATGAGGTTATTGATAAAGATGATAAGTTGGACTCCAGATCTCGTGTGCAACTTGCCAGGTTTTCTTCAATGGAATCTCTGAAGCATGTGGAAACCCGTTCATCAAAGAAAAATAAGAAACTAGAATTTAACAGCAGTCGAGTCTCACCTGTTCCAAATGGAGGATCACAGTGGGGAGCTCTTAATATTTCTAAACCTTTTAATCCAGTATTTGGGTCATCCAAGAAGTTTTTCTCAGCCTCTGTTCCTGGATCAAGAATTATTTCTCGAGCTACATCCCCAATTTCAAGGCGCCCCAGCCCACCTCGTTCTACAACACCAACTCCAACTCTGGGAGGACTCACATCGCCAAAGATTGTTGTAGATGATGCTAAAAGGACAAATGAAGGCCTCAGACAGGAGGTTATCAAACTAAGATCACAGGTTGAAAGTCTTACTCAAAAAGCCCAACTACAAGAAGTTGAGCTGGAACGAACAACTAAACAGCTGAAGGAAGCAATAGCAATTGCAGGTGCAGAGACTGCAAAATGCAAAGCAGCAAAGGAAGTGATCCAGTCACTTACTGCCCAATTGAAGGACATGGCTGAAAGGCTGCCTGTTGGAGCAGTACGGAATATCAAATCACCCTCTCTAGCTTCTTCCTTGGGTTCTGATCCTTCCAACGAAGTTCCTATTCCTTCGACTGACCGATTGAATGGTCAATTAACATTCCAAGAACCAGACTCCAATGAACCAAACAGTCAGTTGTTTTCTAATGGGTCCAACACCACCAGTAACCGCAATTCAGGTCACAACAAACAAGGACATCTAGATGTGGCAACCAGAAATGGGAACAAAATTAAAGAAAACGAATCTCATCATGAATCTGAATGGGTTGAGCAAGATGAGCCTGGTGTATATATCACACTTACTTCCCTACCGGGAGGCGCCAAGGATCTCAAGCGAGTTCGTTTCAGTCGGAAGCGATTTAGTGAGAAACAAGCAGAACAGTGGTGGGCAGAGAACCGAGCAAGAGTATATGAACAATACAATGTGCGCACAGTAGACAAGTCTAGTGTAGGCGTTGGGAGCGAGGACTTGGCTCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0004842 GO:0005085 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005814 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006303 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006974 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0015031 GO:0015630 GO:0015833 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0031625 GO:0031974 GO:0031981 GO:0032182 GO:0032183 GO:0032446 GO:0033036 GO:0033554 GO:0034613 GO:0034641 GO:0036211 GO:0042886 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0050896 GO:0051020 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0065009 GO:0070013 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0098772 GO:0140096 GO:1901360 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRX BRX_N BRX_assoc FYVE PH_12 RCC1

Protein Analysis

1109

Amino Acids

120.35

Weight (kDa)

9.21

Isoelectric Point (pI)

45.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_12	PF16457	22 - 128	3.4e-09	Pleckstrin homology domain
WD40_RLD	PF25390	269 - 652	1.8e-80	RCC1-like domain
RCC1	PF00415	329 - 378	1.1e-11	Regulator of chromosome condensation (RCC1) repeat
RCC1_2	PF13540	367 - 392	2.2e-08	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	381 - 433	4.1e-11	Regulator of chromosome condensation (RCC1) repeat
RCC1_2	PF13540	420 - 448	8e-06	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	437 - 485	2.7e-14	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	501 - 548	4.3e-13	Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD	PF25384	502 - 603	8.9e-06	Alsin RCC1-like domain
RCC1	PF00415	552 - 601	5.3e-09	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	606 - 653	9.7e-15	Regulator of chromosome condensation (RCC1) repeat
FYVE	PF01363	657 - 723	1.1e-15	FYVE zinc finger
BRX_N	PF13713	919 - 953	1.2e-14	Transcription factor BRX N-terminal domain
BRX_assoc	PF16627	960 - 1028	2.6e-21	Unstructured region between BRX_N and BRX domain
BRX	PF08381	1034 - 1089	8e-30	Transcription factor regulating root and shoot growth via Pin3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007030)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42680
fragaria_vesca	FvH4_2g19022 FvH4_3g28000 FvH4_3g28000 FvH4_3g28000 FvH4_5g11302 FvH4_5g34171 FvH4_6g23262
malus_domestica	MD03G1167700.v1.1 MD11G1186800.v1.1
prunus_persica	Prupe.4G268000_v2.0.a1
pyrus_communis	pycom03g12290 pycom03g12300 pycom11g16130 pycom11g16140
rosa_chinensis	RchiOBHm_Chr5g0050701
rosa_laevigata	RLG00000034724
rosa_multiflora	Rmu_sc0002117.1_g000031
rosa_roxburghii	Rroxscaffold_1G00030080
rosa_rugosa	Rorug05G0263200
rosa_samantha	Rh5AG335200 Rh5BG344400 Rh5CG372500 Rh5DG357600
rosa_wichuraiana	Rw5G031620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2052
AarI	CACCTGC	1 cut(s)	2738
AatII	GACGTC	1 cut(s)	939
Acc16I	TGCGCA	1 cut(s)	3275
Acc36I	ACCTGC	1 cut(s)	2738
AccB1I	GGYRCC	4 cut(s)	1781, 2113, 2529, 3164
AccB7I	CCANNNNNTGG	1 cut(s)	2450
AccBSI	CCGCTC	1 cut(s)	1589
AccI	GTMKAC	4 cut(s)	1186, 1665, 1823, 3282
AccII	CGCG	2 cut(s)	7, 469
AciI	CCGC	6 cut(s)	7, 388, 469, 1587, 1935, 3016
AclWI	GGATC	6 cut(s)	36, 2410, 2497, 2782, 2885, 3180
AcoI	YGGCCR	3 cut(s)	648, 1351, 1690
AcsI	RAATTY	5 cut(s)	432, 487, 1474, 1879, 2363
AcuI	CTGAAG	6 cut(s)	462, 624, 1421, 1836, 2339, 2747
AcvI	CACGTG	1 cut(s)	217
AcyI	GRCGYC	3 cut(s)	936, 2530, 3165
AfaI	GTAC	9 cut(s)	284, 663, 696, 930, 1118, 1154, 1336, 1648, 2847
AfiI	CCNNNNNNNGG	9 cut(s)	575, 626, 1688, 2399, 2450, 2570, 2635, 2812, 3066
AflII	CTTAAG	1 cut(s)	203
AflIII	ACRYGT	1 cut(s)	216
AjiI	CACGTC	1 cut(s)	1663
AjnI	CCWGG	5 cut(s)	1280, 1382, 2294, 2485, 3127
AjuI	GAANNNNNNNTTGG	6 cut(s)	617, 649, 1452, 1484, 2453, 2485
Alw21I	GWGCWC	1 cut(s)	2419
Alw26I	GTCTC	4 cut(s)	51, 1264, 2386, 2750
AlwI	GGATC	6 cut(s)	36, 2410, 2497, 2782, 2885, 3180
AlwNI	CAGNNNCTG	3 cut(s)	1491, 2480, 2759
Ama87I	CYCGRG	2 cut(s)	1731, 2505
AoxI	GGCC	7 cut(s)	43, 269, 648, 1351, 1405, 1690, 2626
ApeKI	GCWGC	9 cut(s)	390, 968, 1424, 1448, 2007, 2082, 2174, 2774, 2830
ApoI	RAATTY	5 cut(s)	432, 487, 1474, 1879, 2363
Asp700I	GAANNNNTTC	5 cut(s)	22, 165, 229, 2468, 2500
AspLEI	GCGC	3 cut(s)	2532, 3167, 3276
AspS9I	GGNCC	8 cut(s)	9, 39, 44, 1219, 1238, 1277, 1405, 2997
AsuC2I	CCSGG	2 cut(s)	2012, 3159
AvaI	CYCGRG	2 cut(s)	1731, 2505
AvaII	GGWCC	6 cut(s)	9, 39, 1219, 1238, 1277, 2997
BalI	TGGCCA	2 cut(s)	1353, 1692
BanI	GGYRCC	4 cut(s)	1781, 2113, 2529, 3164
BanII	GRGCYC	2 cut(s)	686, 2419
BauI	CACGAG	1 cut(s)	2280
BbrPI	CACGTG	1 cut(s)	217
Bbv12I	GWGCWC	1 cut(s)	2419
BbvCI	CCTCAGC	1 cut(s)	351
BbvI	GCAGC	9 cut(s)	402, 955, 1435, 1436, 1994, 2094, 2161, 2786, 2817
BccI	CCATC	9 cut(s)	402, 422, 445, 782, 830, 1160, 1325, 1517, 1709
BceAI	ACGGC	2 cut(s)	284, 1277
BciT130I	CCWGG	5 cut(s)	1282, 1384, 2296, 2487, 3129
BciVI	GTATCC	1 cut(s)	273
BclI	TGATCA	1 cut(s)	1987
BcnI	CCSGG	2 cut(s)	2012, 3159
BcoDI	GTCTC	4 cut(s)	51, 1264, 2386, 2750
BfaI	CTAG	8 cut(s)	222, 320, 456, 2124, 2360, 2870, 3047, 3291
BfmI	CTRYAG	2 cut(s)	1449, 1950
BfoI	RGCGCY	2 cut(s)	2533, 3168
BfrI	CTTAAG	1 cut(s)	203
BfuAI	ACCTGC	1 cut(s)	2738
BfuI	GTATCC	1 cut(s)	273
BglII	AGATCT	1 cut(s)	2276
Bme1390I	CCNGG	7 cut(s)	1282, 1384, 2012, 2296, 2487, 3129, 3159
Bme18I	GGWCC	6 cut(s)	9, 39, 1219, 1238, 1277, 2997
BmeT110I	CYCGRG	2 cut(s)	1731, 2505
BmgBI	CACGTC	1 cut(s)	1663
BmgT120I	GGNCC	8 cut(s)	9, 39, 44, 1219, 1238, 1277, 1405, 2997
BmrFI	CCNGG	7 cut(s)	1282, 1384, 2012, 2296, 2487, 3129, 3159
BmsI	GCATC	4 cut(s)	715, 784, 1063, 2596
BplI	GAGNNNNNCTC	4 cut(s)	463, 495, 983, 1015
BpmI	CTGGAG	1 cut(s)	2257
Bpu10I	CCTNAGC	1 cut(s)	351
BpuEI	CTTGAG	3 cut(s)	89, 1692, 3161
BpuMI	CCSGG	2 cut(s)	2012, 3159
BsaAI	YACGTR	1 cut(s)	217
BsaHI	GRCGYC	3 cut(s)	936, 2530, 3165
BsaXI	ACNNNNNCTCC	6 cut(s)	272, 302, 825, 855, 2545, 2575
Bsc4I	CCNNNNNNNGG	9 cut(s)	575, 626, 1688, 2399, 2450, 2570, 2635, 2812, 3066
Bse118I	RCCGGY	1 cut(s)	41
Bse1I	ACTGG	7 cut(s)	286, 651, 747, 1693, 2444, 2791, 3009
Bse3DI	GCAATG	3 cut(s)	720, 726, 945
BseBI	CCWGG	5 cut(s)	1282, 1384, 2296, 2487, 3129
BseGI	GGATG	5 cut(s)	730, 1048, 1262, 2456, 2513
BseLI	CCNNNNNNNGG	9 cut(s)	575, 626, 1688, 2399, 2450, 2570, 2635, 2812, 3066
BseMI	GCAATG	3 cut(s)	720, 726, 945
BseMII	CTCAG	6 cut(s)	264, 342, 699, 2156, 2487, 2644
BseNI	ACTGG	7 cut(s)	286, 651, 747, 1693, 2444, 2791, 3009
BseRI	GAGGAG	1 cut(s)	471
BseXI	GCAGC	9 cut(s)	402, 955, 1435, 1436, 1994, 2094, 2161, 2786, 2817
BseYI	CCCAGC	2 cut(s)	1307, 2534
BsgI	GTGCAG	2 cut(s)	549, 2772
Bsh1236I	CGCG	2 cut(s)	7, 469
BshFI	GGCC	7 cut(s)	45, 271, 650, 1353, 1407, 1692, 2628
BshNI	GGYRCC	4 cut(s)	1781, 2113, 2529, 3164
BsiHKAI	GWGCWC	1 cut(s)	2419
BsiHKCI	CYCGRG	2 cut(s)	1731, 2505
BsiSI	CCGG	3 cut(s)	42, 2011, 3158
BslFI	GGGAC	1 cut(s)	1205
BslI	CCNNNNNNNGG	9 cut(s)	575, 626, 1688, 2399, 2450, 2570, 2635, 2812, 3066
BsmAI	GTCTC	4 cut(s)	51, 1264, 2386, 2750
BsmFI	GGGAC	1 cut(s)	1205
BsnI	GGCC	7 cut(s)	45, 271, 650, 1353, 1407, 1692, 2628
BsoBI	CYCGRG	2 cut(s)	1731, 2505
Bsp1286I	GDGCHC	2 cut(s)	686, 2419
Bsp1407I	TGTACA	2 cut(s)	694, 1152
Bsp19I	CCATGG	2 cut(s)	1354, 2109
BspACI	CCGC	6 cut(s)	7, 388, 469, 1587, 1935, 3016
BspANI	GGCC	7 cut(s)	45, 271, 650, 1353, 1407, 1692, 2628
BspCNI	CTCAG	6 cut(s)	265, 343, 698, 2157, 2486, 2643
BspFNI	CGCG	2 cut(s)	7, 469
BspHI	TCATGA	2 cut(s)	781, 3097
BspMAI	CTGCAG	2 cut(s)	1453, 1954
BspMI	ACCTGC	1 cut(s)	2738
BspPI	GGATC	6 cut(s)	36, 2410, 2497, 2782, 2885, 3180
BspT107I	GGYRCC	4 cut(s)	1781, 2113, 2529, 3164
BspTI	CTTAAG	1 cut(s)	203
BsrBI	CCGCTC	1 cut(s)	1589
BsrDI	GCAATG	3 cut(s)	720, 726, 945
BsrFI	RCCGGY	1 cut(s)	41
BsrGI	TGTACA	2 cut(s)	694, 1152
BsrI	ACTGG	7 cut(s)	286, 651, 747, 1693, 2444, 2791, 3009
BssAI	RCCGGY	1 cut(s)	41
BssNAI	GTATAC	1 cut(s)	1824
BssNI	GRCGYC	3 cut(s)	936, 2530, 3165
BssSI	CACGAG	1 cut(s)	2280
BssT1I	CCWWGG	7 cut(s)	624, 769, 1242, 1354, 2109, 2880, 3168
Bst1107I	GTATAC	1 cut(s)	1824
Bst2BI	CACGAG	1 cut(s)	2280
Bst2UI	CCWGG	5 cut(s)	1282, 1384, 2296, 2487, 3129
Bst4CI	ACNGT	9 cut(s)	609, 692, 1417, 1493, 1639, 2409, 2978, 3228, 3280
Bst6I	CTCTTC	2 cut(s)	1761, 2209
BstACI	GRCGYC	3 cut(s)	936, 2530, 3165
BstAFI	CTTAAG	1 cut(s)	203
BstAPI	GCANNNNNTGC	2 cut(s)	725, 2759
BstAUI	TGTACA	2 cut(s)	694, 1152
BstBAI	YACGTR	1 cut(s)	217
BstC8I	GCNNGC	6 cut(s)	69, 966, 1025, 1422, 1486, 1933
BstDSI	CCRYGG	3 cut(s)	266, 1354, 2109
BstENI	CCTNNNNNAGG	1 cut(s)	2633
BstF5I	GGATG	5 cut(s)	730, 1048, 1262, 2456, 2513
BstFNI	CGCG	2 cut(s)	7, 469
BstH2I	RGCGCY	2 cut(s)	2533, 3168
BstHHI	GCGC	3 cut(s)	2532, 3167, 3276
BstMAI	GTCTC	4 cut(s)	51, 1264, 2386, 2750
BstNI	CCWGG	5 cut(s)	1282, 1384, 2296, 2487, 3129
BstNSI	RCATGY	3 cut(s)	968, 1259, 2327
BstSCI	CCNGG	7 cut(s)	1280, 1382, 2010, 2294, 2485, 3127, 3157
BstSFI	CTRYAG	2 cut(s)	1449, 1950
BstUI	CGCG	2 cut(s)	7, 469
BstV1I	GCAGC	9 cut(s)	402, 955, 1435, 1436, 1994, 2094, 2161, 2786, 2817
BstX2I	RGATCY	3 cut(s)	28, 2276, 3172
BstXI	CCANNNNNNTGG	2 cut(s)	776, 1390
BstYI	RGATCY	3 cut(s)	28, 2276, 3172
BstZ17I	GTATAC	1 cut(s)	1824
BsuI	GTATCC	1 cut(s)	273
BsuRI	GGCC	7 cut(s)	45, 271, 650, 1353, 1407, 1692, 2628
BtgI	CCRYGG	3 cut(s)	266, 1354, 2109
BtgZI	GCGATG	1 cut(s)	2568
BtrI	CACGTC	1 cut(s)	1663
BtsCI	GGATG	5 cut(s)	730, 1048, 1262, 2456, 2513
BtsIMutI	CAGTG	7 cut(s)	658, 697, 754, 1413, 2414, 3233, 3322
BveI	ACCTGC	1 cut(s)	2738
Cac8I	GCNNGC	6 cut(s)	69, 966, 1025, 1422, 1486, 1933
CaiI	CAGNNNCTG	3 cut(s)	1491, 2480, 2759
CciI	TCATGA	2 cut(s)	781, 3097
CfoI	GCGC	3 cut(s)	2532, 3167, 3276
Cfr10I	RCCGGY	1 cut(s)	41
Cfr13I	GGNCC	8 cut(s)	9, 39, 44, 1219, 1238, 1277, 1405, 2997
CpoI	CGGWCCG	1 cut(s)	9
Csp6I	GTAC	9 cut(s)	283, 662, 695, 929, 1117, 1153, 1335, 1647, 2846
CspI	CGGWCCG	1 cut(s)	9
CviQI	GTAC	9 cut(s)	283, 662, 695, 929, 1117, 1153, 1335, 1647, 2846
DinI	GGCGCC	2 cut(s)	2531, 3166
DraI	TTTAAA	1 cut(s)	2029
EaeI	YGGCCR	3 cut(s)	648, 1351, 1690
Eam1104I	CTCTTC	2 cut(s)	1761, 2209
EarI	CTCTTC	2 cut(s)	1761, 2209
Ecl136II	GAGCTC	1 cut(s)	2417
Eco130I	CCWWGG	7 cut(s)	624, 769, 1242, 1354, 2109, 2880, 3168
Eco147I	AGGCCT	1 cut(s)	2628
Eco24I	GRGCYC	2 cut(s)	686, 2419
Eco32I	GATATC	1 cut(s)	384
Eco47I	GGWCC	6 cut(s)	9, 39, 1219, 1238, 1277, 2997
Eco53kI	GAGCTC	1 cut(s)	2417
Eco57I	CTGAAG	6 cut(s)	462, 624, 1421, 1836, 2339, 2747
Eco72I	CACGTG	1 cut(s)	217
Eco88I	CYCGRG	2 cut(s)	1731, 2505
EcoICRI	GAGCTC	1 cut(s)	2417
EcoNI	CCTNNNNNAGG	1 cut(s)	2633
EcoO109I	RGGNCCY	2 cut(s)	1219, 1405
EcoRI	GAATTC	3 cut(s)	432, 1474, 1879
EcoRII	CCWGG	5 cut(s)	1280, 1382, 2294, 2485, 3127
EcoRV	GATATC	1 cut(s)	384
EcoT14I	CCWWGG	7 cut(s)	624, 769, 1242, 1354, 2109, 2880, 3168
EcoT22I	ATGCAT	1 cut(s)	1257
EcoT38I	GRGCYC	2 cut(s)	686, 2419
EgeI	GGCGCC	2 cut(s)	2531, 3166
EheI	GGCGCC	2 cut(s)	2531, 3166
ErhI	CCWWGG	7 cut(s)	624, 769, 1242, 1354, 2109, 2880, 3168
FalI	AAGNNNNNCTT	8 cut(s)	617, 649, 915, 947, 1551, 1583, 1915, 1947
FaqI	GGGAC	1 cut(s)	1205
FauI	CCCGC	1 cut(s)	395
FbaI	TGATCA	1 cut(s)	1987
FblI	GTMKAC	4 cut(s)	1186, 1665, 1823, 3282
FokI	GGATG	5 cut(s)	737, 1055, 1249, 2443, 2500
FriOI	GRGCYC	2 cut(s)	686, 2419
FspAI	RTGCGCAY	1 cut(s)	3275
FspBI	CTAG	8 cut(s)	222, 320, 456, 2124, 2360, 2870, 3047, 3291
FspI	TGCGCA	1 cut(s)	3275
GlaI	GCGC	3 cut(s)	2531, 3166, 3275
GsaI	CCCAGC	2 cut(s)	1311, 2538
GsuI	CTGGAG	1 cut(s)	2257
HaeII	RGCGCY	2 cut(s)	2533, 3168
HaeIII	GGCC	7 cut(s)	45, 271, 650, 1353, 1407, 1692, 2628
HapII	CCGG	3 cut(s)	42, 2011, 3158
HhaI	GCGC	3 cut(s)	2532, 3167, 3276
Hin1I	GRCGYC	3 cut(s)	936, 2530, 3165
Hin6I	GCGC	3 cut(s)	2530, 3165, 3274
HinP1I	GCGC	3 cut(s)	2530, 3165, 3274
HincII	GTYRAC	1 cut(s)	1048
HindII	GTYRAC	1 cut(s)	1048
HindIII	AAGCTT	2 cut(s)	1721, 2103
HpaII	CCGG	3 cut(s)	42, 2011, 3158
Hpy99I	CGWCG	1 cut(s)	53
HpyCH4III	ACNGT	9 cut(s)	609, 692, 1417, 1493, 1639, 2409, 2978, 3228, 3280
HpyCH4IV	ACGT	4 cut(s)	216, 936, 1050, 1662
HpySE526I	ACGT	4 cut(s)	216, 936, 1050, 1662
Hsp92I	GRCGYC	3 cut(s)	936, 2530, 3165
HspAI	GCGC	3 cut(s)	2530, 3165, 3274
KasI	GGCGCC	2 cut(s)	2529, 3164
KflI	GGGWCCC	1 cut(s)	1219
Ksp22I	TGATCA	1 cut(s)	1987
LmnI	GCTCC	4 cut(s)	1838, 2414, 2840, 3306
Lsp1109I	GCAGC	9 cut(s)	402, 955, 1435, 1436, 1994, 2094, 2161, 2786, 2817
LweI	GCATC	4 cut(s)	715, 784, 1063, 2596
MaeI	CTAG	8 cut(s)	222, 320, 456, 2124, 2360, 2870, 3047, 3291
MaeII	ACGT	4 cut(s)	216, 936, 1050, 1662
MaeIII	GTNAC	9 cut(s)	315, 976, 1132, 1297, 1550, 1658, 2793, 3011, 3026
MbiI	CCGCTC	1 cut(s)	1589
MboII	GAAGA	6 cut(s)	126, 625, 1778, 2226, 2295, 2868
MfeI	CAATTG	2 cut(s)	2742, 2807
MflI	RGATCY	3 cut(s)	28, 2276, 3172
MhlI	GDGCHC	2 cut(s)	686, 2419
MlsI	TGGCCA	2 cut(s)	1353, 1692
MluNI	TGGCCA	2 cut(s)	1353, 1692
Mly113I	GGCGCC	2 cut(s)	2530, 3165
MlyI	GAGTC	6 cut(s)	238, 425, 2264, 2388, 2571, 2954
MmeI	TCCRAC	8 cut(s)	847, 1751, 2247, 2588, 2818, 2922, 3024, 3176
Mox20I	TGGCCA	2 cut(s)	1353, 1692
Mph1103I	ATGCAT	1 cut(s)	1257
MroXI	GAANNNNTTC	5 cut(s)	22, 165, 229, 2468, 2500
MscI	TGGCCA	2 cut(s)	1353, 1692
MslI	CAYNNNNRTG	4 cut(s)	786, 1125, 1200, 3048
Msp20I	TGGCCA	2 cut(s)	1353, 1692
MspA1I	CMGCKG	1 cut(s)	2725
MspCI	CTTAAG	1 cut(s)	203
MspI	CCGG	3 cut(s)	42, 2011, 3158
MspR9I	CCNGG	7 cut(s)	1282, 1384, 2012, 2296, 2487, 3129, 3159
MunI	CAATTG	2 cut(s)	2742, 2807
MvaI	CCWGG	5 cut(s)	1282, 1384, 2296, 2487, 3129
MvnI	CGCG	2 cut(s)	7, 469
NarI	GGCGCC	2 cut(s)	2530, 3165
NciI	CCSGG	2 cut(s)	2012, 3159
NcoI	CCATGG	2 cut(s)	1354, 2109
NmuCI	GTSAC	5 cut(s)	315, 1550, 1658, 2793, 3026
NsbI	TGCGCA	1 cut(s)	3275
NsiI	ATGCAT	1 cut(s)	1257
NspI	RCATGY	3 cut(s)	968, 1259, 2327
PaeI	GCATGC	1 cut(s)	968
PaeR7I	CTCGAG	2 cut(s)	1731, 2505
PagI	TCATGA	2 cut(s)	781, 3097
PaqCI	CACCTGC	1 cut(s)	2738
PasI	CCCWGGG	1 cut(s)	1383
PceI	AGGCCT	1 cut(s)	2628
PcsI	WCGNNNNNNNCGW	1 cut(s)	1417
PdmI	GAANNNNTTC	5 cut(s)	22, 165, 229, 2468, 2500
PflMI	CCANNNNNTGG	1 cut(s)	2450
PfoI	TCCNGGA	1 cut(s)	2485
PleI	GAGTC	6 cut(s)	238, 424, 2264, 2387, 2571, 2954
PluTI	GGCGCC	2 cut(s)	2533, 3168
PmaCI	CACGTG	1 cut(s)	217
PmlI	CACGTG	1 cut(s)	217
PpsI	GAGTC	6 cut(s)	238, 424, 2264, 2387, 2571, 2954
Ppu21I	YACGTR	1 cut(s)	217
PpuMI	RGGWCCY	1 cut(s)	1219
PsiI	TTATAA	1 cut(s)	2052
Psp124BI	GAGCTC	1 cut(s)	2419
Psp5II	RGGWCCY	1 cut(s)	1219
Psp6I	CCWGG	5 cut(s)	1280, 1382, 2294, 2485, 3127
PspCI	CACGTG	1 cut(s)	217
PspFI	CCCAGC	2 cut(s)	1307, 2534
PspGI	CCWGG	5 cut(s)	1280, 1382, 2294, 2485, 3127
PspPI	GGNCC	8 cut(s)	9, 39, 44, 1219, 1238, 1277, 1405, 2997
PspPPI	RGGWCCY	1 cut(s)	1219
PspXI	VCTCGAGB	1 cut(s)	1731
PstI	CTGCAG	2 cut(s)	1453, 1954
PstNI	CAGNNNCTG	3 cut(s)	1491, 2480, 2759
PsuI	RGATCY	3 cut(s)	28, 2276, 3172
PvuII	CAGCTG	1 cut(s)	2725
RsaI	GTAC	9 cut(s)	284, 663, 696, 930, 1118, 1154, 1336, 1648, 2847
RsaNI	GTAC	9 cut(s)	283, 662, 695, 929, 1117, 1153, 1335, 1647, 2846
RseI	CAYNNNNRTG	4 cut(s)	786, 1125, 1200, 3048
Rsr2I	CGGWCCG	1 cut(s)	9
RsrII	CGGWCCG	1 cut(s)	9
SacI	GAGCTC	1 cut(s)	2419
Sau96I	GGNCC	8 cut(s)	9, 39, 44, 1219, 1238, 1277, 1405, 2997
SchI	GAGTC	6 cut(s)	238, 425, 2264, 2388, 2571, 2954
ScrFI	CCNGG	7 cut(s)	1282, 1384, 2012, 2296, 2487, 3129, 3159
SduI	GDGCHC	2 cut(s)	686, 2419
SfaNI	GCATC	4 cut(s)	715, 784, 1063, 2596
SfcI	CTRYAG	2 cut(s)	1449, 1950
SfoI	GGCGCC	2 cut(s)	2531, 3166
Sfr274I	CTCGAG	2 cut(s)	1731, 2505
SinI	GGWCC	6 cut(s)	9, 39, 1219, 1238, 1277, 2997
SlaI	CTCGAG	2 cut(s)	1731, 2505
SmiMI	CAYNNNNRTG	4 cut(s)	786, 1125, 1200, 3048
SmlI	CTYRAG	6 cut(s)	104, 203, 1707, 1731, 2505, 3176
SmoI	CTYRAG	6 cut(s)	104, 203, 1707, 1731, 2505, 3176
SphI	GCATGC	1 cut(s)	968
SseBI	AGGCCT	1 cut(s)	2628
SsiI	CCGC	6 cut(s)	7, 388, 469, 1587, 1935, 3016
SspDI	GGCGCC	2 cut(s)	2529, 3164
SspI	AATATT	1 cut(s)	2425
SspMI	CTAG	8 cut(s)	222, 320, 456, 2124, 2360, 2870, 3047, 3291
SstI	GAGCTC	1 cut(s)	2419
StuI	AGGCCT	1 cut(s)	2628
StyD4I	CCNGG	7 cut(s)	1280, 1382, 2010, 2294, 2485, 3127, 3157
StyI	CCWWGG	7 cut(s)	624, 769, 1242, 1354, 2109, 2880, 3168
TaaI	ACNGT	9 cut(s)	609, 692, 1417, 1493, 1639, 2409, 2978, 3228, 3280
TaiI	ACGT	4 cut(s)	219, 939, 1053, 1665
TaqI	TCGA	8 cut(s)	51, 63, 400, 1612, 1732, 2377, 2506, 2918
TaqII	GACCGA	3 cut(s)	26, 1172, 2940
TatI	WGTACW	3 cut(s)	694, 928, 1152
TauI	GCSGC	1 cut(s)	1589
TscAI	CASTG	6 cut(s)	658, 697, 754, 1420, 2414, 3233
TseFI	GTSAC	5 cut(s)	315, 1550, 1658, 2793, 3026
TseI	GCWGC	9 cut(s)	390, 968, 1424, 1448, 2007, 2082, 2174, 2774, 2830
Tsp45I	GTSAC	5 cut(s)	315, 1550, 1658, 2793, 3026
TspGWI	ACGGA	4 cut(s)	154, 543, 649, 2863
TspRI	CASTG	6 cut(s)	658, 697, 754, 1420, 2414, 3233
Van91I	CCANNNNNTGG	1 cut(s)	2450
Vha464I	CTTAAG	1 cut(s)	203
VpaK11BI	GGWCC	6 cut(s)	9, 39, 1219, 1238, 1277, 2997
XagI	CCTNNNNNAGG	1 cut(s)	2633
XapI	RAATTY	5 cut(s)	432, 487, 1474, 1879, 2363
XbaI	TCTAGA	2 cut(s)	221, 3046
XceI	RCATGY	3 cut(s)	968, 1259, 2327
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1759
XhoI	CTCGAG	2 cut(s)	1731, 2505
XmiI	GTMKAC	4 cut(s)	1186, 1665, 1823, 3282
XmnI	GAANNNNTTC	5 cut(s)	22, 165, 229, 2468, 2500
XspI	CTAG	8 cut(s)	222, 320, 456, 2124, 2360, 2870, 3047, 3291
ZraI	GACGTC	1 cut(s)	937
Zsp2I	ATGCAT	1 cut(s)	1257

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.