Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005859

Physical Location & Seq

Genomic Coordinates Forward (+)

40543541 .. 40549157

Gene Structure 5617 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1286100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2757 bp

ATGGTACCCCTCGTTTCCCTCCACACATCCACAACCCAACCCCTCCTATATACCCCAACCGCCACCCCATCTCTCCAACCCCACCTCTCCCGCACCAAAGCCCATCCTCCCCGACGAACCCAATTTTTCATTTCATCGATCTCCCAGAACTCTCATCGATTCACCAGCCAAAACCCCCAACCCCCGGAAGCTCAGCCGCCGAGAACCCAGTTCCCAGGTGGGTTCAAGCGCCCGGAGGTCAGGGTCCCCAATGTCGTGCTCCAATTGGACCCCGACGAAGTCCTTGCCGGCGAGGATGCTTTGGATTTGGTAGACAAGGCGGTGTCGAAGTGGGTCGGGGTTCTGGTGCTTAATGGTCGTGATTCAAGCGGCGGCAGGCTCTATGAGGCCGCCTGTAAGCTGAAGTCTGTGATTAGAGACCGCGCCTACTTGTTGATCTCCGAGAGGGTCGATATCGCCGCCGCAGCTAATGCCAGTGGAGTTCTGCTCTCCGATCAAGGTCTTCCTACCATTGTAGCAAGAAGCACAATGATGGCTTCTAAATCTGATTTGGTTGTCCTTCCTTTGGTGGCAAGATACGTGCAAGATATAGATGGTGCCATAAATGCATCCAACTCTGAAGGAGCTGACTTTCTTATATACGATATTGCCGGACAGGAAAATATTCTTCTGGCACTCAACAGTCTGTTTAAAACTGTAAAGATACCAATATTTGTCACGTTCACTTCTTATAACGCCTTATATAAGGAGGGGCCAGCATTATTGAAATCTGGCGCTAGTGGATTAGTTACGTCTTTGAAAGATTTCCGGCTGCTTGATGATAAGGCTTTGAGCAAATTGTTTGACATCGTGTACATGCCGAATAGTGAAACACAGGATGAAGTTGAAAGCTTAAGTGAGCTCACGTATTTGGATGCCCATGATGGTCCCATCGACAGTACAAGTGTTGCTGGTTTTCTTAAATTGGAGGATAGAGAAAAAAAGTTCATAGAAGCAGAAAGATCAGTGTTGCTTAAAGCAATAAATGTCATCCAGAAAGCTGCTCCACTGATGGAGGAGGTTTCACTTCTCGTTGATGCAGTTTCTCAAATTGATGAGCCATTTTTGCTGGTTATAGTGGGTGAATTTAACTCTGGCAAGTCAACTGTTATTAATGCCCTTCTTGGGAATAAATATCTTAAAGACGGGGTTGTTCCTACAACTAATGAGATTACTTTCTTACGGTATACTGAGATGGATGCTGGTGAGGAACAGCGTTGTGAAAGGCATCCAGATGGTCAATATATGTGCTACCTTCCTGCTCCGATTCTTAAAGAAATGAACGTTGTTGATACACCTGGAACTAATGTGATTCTCCAAAGGCAACAACGCCTTACTGAGGAATTTGTGCCCCGTGCCGATTTGCTTCTTTTTGTCATCTCTGCTGATCGCCCGTTAACTGAAAGTGAGGTTGTGTTTCTTCGTTATACTCAGCAATGGAAGAAGAAAGTTGTGTTTGTCTTGAATAAATCTGATATCTACCGCAATGCCCATGAGCTTGAGGAAGCTATGTCATTCATCGAGAAGAATACACAAAAACTTTTGAATACTGAACATGTTACTTTATTTCCTGTGTCTGCACGCTCTGCTCTTGAAGCAAAACTTTCAGCTTCTATGTTTGGGAAAGATTGTGCAGAACTATCGACATCCGATGCTCAGTGGAAAAGTAGTAATTTCTCTGAACTTGAGAATTTCTTGTATAGTTTCTTAGACGGGTCAACAAGCACAGGAATGGAAAGAATGAAAATTAAACTGGAAACACCGGTAGCGATTGCAGAAAAGCTACTTTCTGCTTGTGAAACTCTTGTGACACAGGACTGCCGATATGCCAAGCAGGACTTGGCCTCAATAAATGATATAGTTGGCAGTGTGAAGAACTATGCAGTTAAGATGGAAAATGAAAGCATAGCTTGGAGAAGAAGAATTTTATCTGTGATTGATATTACAAAATCACGTGTTGTTGAACTCATAGAAGCTACTCTGCAATTATCAAATCTTGATCTTGTTGCTAACTATGTTTTCAAAGGGGAAAAATCCTCCACGATTGCTACCACCTTGAGGGTTCAAAATGACATAATGGGTCCTGCATTTTCAGATGCGCAAAAACAACTTGGAGAATATGTGACATGGTTGCAATCAGACAGTGCTCGCGAAGGAAGGATGTATGCAGAAATGTTTGAAAAACGTTGGCCTTCATTTGTCTATCCATACAGTGGGGTGCATTCGGTGAAGAAAGTAAACGAACTCAGCTTGAAAGTGATAGAGGGCTTCAGTACCAGTGCTGCTTCCAAATTGTTTGAGCAAGAAATACGTGAAGTGTCTCTGGCAACTTTTGGTGGACTTGGAGCGGCTGGTTTATCTGCTTCACTTCTGACGTCTGTGCTGCCAAACACATTAGAAGATCTTCTTGCTCTTGGTCTTGCCTCAGCTGGCGGGTTATTAGCAATCTCAAAATTTCCAGCTCGTCGGCAAGAGATGATAGAGAAGGTGAAAAGGACTGCAGACGCCTTGGCACGTGAAGTTGAAGAGGCCATGCAGAATGATCTTTCCGAAGCTATTGGAAATATGGAAATCTTCGTGAAGAACGTTAGCCAGCCTTACCAAGATACGGCGCAGCAGAGACTAGACAAACTCCTAGAGCTTCAAGATGAAATATCCAATGTTGATAAACAACTTCAAACACTTCGAATTGAAATCCAAAATCTTCACGTATCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002682 GO:0002683 GO:0002831 GO:0002832 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007275 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009657 GO:0009668 GO:0009707 GO:0009791 GO:0009941 GO:0009987 GO:0010027 GO:0010228 GO:0010363 GO:0010941 GO:0016020 GO:0016043 GO:0019867 GO:0022414 GO:0031090 GO:0031347 GO:0031348 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0034051 GO:0042170 GO:0043067 GO:0043069 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043901 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045088 GO:0045824 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0060548 GO:0061024 GO:0061458 GO:0065007 GO:0071840 GO:0080134 GO:0080135 GO:0098588 GO:0098805 GO:1900424 GO:1900425 GO:1902477 GO:1902478

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dynamin_N TMP-TENI

Protein Analysis

919

Amino Acids

101.8

Weight (kDa)

5.21

Isoelectric Point (pI)

39.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TMP-TENI	PF02581	97 - 177	6.8e-06	Thiamine monophosphate synthase
Dynamin_N	PF00350	370 - 417	6.1e-10	Dynamin family
GTP_EFTU	PF00009	370 - 547	2.7e-07	Elongation factor Tu GTP binding domain
MMR_HSR1	PF01926	371 - 503	3.6e-13	50S ribosome-binding GTPase
Dynamin_N	PF00350	430 - 503	1e-08	Dynamin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005859)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03160 AT1G03160 AT1G03160 AT1G03160
fragaria_vesca	FvH4_2g23600 FvH4_2g23600 FvH4_2g23600 FvH4_2g23600 FvH4_2g23600
malus_domestica	MD00G1063200.v1.1 MD11G1286100.v1.1 MD11G1311300.v1.1 MD11G1311400.v1.1 MD11G1311600.v1.1 MD11G1311700.v1.1 MD11G1312200.v1.1
prunus_persica	Prupe.8G241300_v2.0.a1
pyrus_communis	pycom11g25360
rosa_chinensis	RchiOBHm_Chr6g0291071
rosa_laevigata	RLG00000012149
rosa_multiflora	Rmu_co8364727.1_g000001
rosa_roxburghii	Rroxscaffold_7G00176400
rosa_rugosa	Rorug06G0217300
rosa_samantha	Rh6AG327500 Rh6BG335000 Rh6CG341200 Rh6DG328500
rosa_wichuraiana	Rw6G028490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	732
AatII	GACGTC	1 cut(s)	2417
Acc16I	TGCGCA	1 cut(s)	2139
Acc65I	GGTACC	1 cut(s)	4
AccB1I	GGYRCC	2 cut(s)	4, 596
AccB7I	CCANNNNNTGG	1 cut(s)	2252
AccBSI	CCGCTC	1 cut(s)	2387
AccI	GTMKAC	2 cut(s)	312, 1226
AccII	CGCG	2 cut(s)	423, 2190
AclI	AACGTT	3 cut(s)	1323, 2224, 2625
AcsI	RAATTY	5 cut(s)	1124, 1382, 1729, 1962, 2492
AcuI	CTGAAG	3 cut(s)	422, 639, 2293
AcvI	CACGTG	2 cut(s)	1994, 2555
AcyI	GRCGYC	2 cut(s)	2414, 2544
AfaI	GTAC	4 cut(s)	6, 854, 940, 2314
AfiI	CCNNNNNNNGG	7 cut(s)	184, 565, 1164, 1378, 2097, 2252, 2647
AflII	CTTAAG	1 cut(s)	892
AflIII	ACRYGT	2 cut(s)	1594, 1993
AgeI	ACCGGT	1 cut(s)	1801
AjnI	CCWGG	2 cut(s)	214, 1336
Alw21I	GWGCWC	3 cut(s)	261, 903, 2188
Alw26I	GTCTC	3 cut(s)	411, 2364, 2653
AoxI	GGCC	5 cut(s)	387, 752, 1881, 2228, 2568
ApeKI	GCWGC	6 cut(s)	464, 811, 1040, 2321, 2422, 2653
ApoI	RAATTY	5 cut(s)	1124, 1382, 1729, 1962, 2492
AseI	ATTAAT	1 cut(s)	1152
AsiGI	ACCGGT	1 cut(s)	1801
Asp700I	GAANNNNTTC	3 cut(s)	663, 803, 2442
Asp718I	GGTACC	1 cut(s)	4
AspLEI	GCGC	5 cut(s)	231, 425, 776, 2140, 2653
AspS9I	GGNCC	5 cut(s)	244, 268, 752, 926, 2120
AsuC2I	CCSGG	2 cut(s)	185, 233
AsuHPI	GGTGA	5 cut(s)	154, 1133, 1256, 2278, 2539
AsuII	TTCGAA	1 cut(s)	2725
AvaII	GGWCC	4 cut(s)	244, 268, 926, 2120
BaeGI	GKGCMC	1 cut(s)	1392
BanI	GGYRCC	2 cut(s)	4, 596
BanII	GRGCYC	1 cut(s)	903
BbrPI	CACGTG	2 cut(s)	1994, 2555
BbsI	GAAGAC	1 cut(s)	494
Bbv12I	GWGCWC	3 cut(s)	261, 903, 2188
BbvCI	CCTCAGC	1 cut(s)	2464
BbvI	GCAGC	6 cut(s)	476, 798, 1027, 2308, 2409, 2665
BceAI	ACGGC	1 cut(s)	2664
BcgI	CGANNNNNNTGC	4 cut(s)	266, 300, 1662, 1696
BciT130I	CCWGG	2 cut(s)	216, 1338
BcnI	CCSGG	2 cut(s)	185, 233
BcoDI	GTCTC	3 cut(s)	411, 2364, 2653
BfaI	CTAG	3 cut(s)	777, 2663, 2675
BfmI	CTRYAG	1 cut(s)	2538
BfoI	RGCGCY	2 cut(s)	232, 777
BfrI	CTTAAG	1 cut(s)	892
BglII	AGATCT	1 cut(s)	2440
BlpI	GCTNAGC	1 cut(s)	192
Bme1390I	CCNGG	4 cut(s)	185, 216, 233, 1338
Bme18I	GGWCC	4 cut(s)	244, 268, 926, 2120
BmgT120I	GGNCC	5 cut(s)	244, 268, 752, 926, 2120
BmiI	GGNNCC	8 cut(s)	6, 245, 246, 270, 598, 753, 928, 2121
BmrFI	CCNGG	4 cut(s)	185, 216, 233, 1338
BmrI	ACTGGG	1 cut(s)	202
BmsI	GCATC	8 cut(s)	286, 617, 904, 1066, 1228, 1276, 1681, 2125
BmuI	ACTGGG	1 cut(s)	202
BpiI	GAAGAC	1 cut(s)	494
BplI	GAGNNNNNCTC	2 cut(s)	471, 503
Bpu10I	CCTNAGC	1 cut(s)	2464
Bpu1102I	GCTNAGC	1 cut(s)	192
Bpu14I	TTCGAA	1 cut(s)	2725
BpuEI	CTTGAG	3 cut(s)	1559, 1745, 2116
BpuMI	CCSGG	2 cut(s)	185, 233
Bsa29I	ATCGAT	2 cut(s)	137, 157
BsaAI	YACGTR	6 cut(s)	580, 906, 1994, 2351, 2555, 2749
BsaHI	GRCGYC	2 cut(s)	2414, 2544
BsaI	GGTCTC	1 cut(s)	411
BsaJI	CCNNGG	3 cut(s)	183, 214, 2547
BsaWI	WCCGGW	1 cut(s)	1801
BsaXI	ACNNNNNCTCC	2 cut(s)	2145, 2175
Bsc4I	CCNNNNNNNGG	7 cut(s)	184, 565, 1164, 1378, 2097, 2252, 2647
Bse118I	RCCGGY	2 cut(s)	287, 1801
Bse1I	ACTGG	4 cut(s)	208, 474, 1797, 2316
Bse3DI	GCAATG	2 cut(s)	1481, 1531
BseBI	CCWGG	2 cut(s)	216, 1338
BseCI	ATCGAT	2 cut(s)	137, 157
BseDI	CCNNGG	3 cut(s)	183, 214, 2547
BseLI	CCNNNNNNNGG	7 cut(s)	184, 565, 1164, 1378, 2097, 2252, 2647
BseMI	GCAATG	2 cut(s)	1481, 1531
BseMII	CTCAG	7 cut(s)	206, 1221, 1368, 1484, 1709, 2299, 2478
BseNI	ACTGG	4 cut(s)	208, 474, 1797, 2316
BseRI	GAGGAG	1 cut(s)	1070
BseSI	GKGCMC	1 cut(s)	1392
BseXI	GCAGC	6 cut(s)	476, 798, 1027, 2308, 2409, 2665
BsgI	GTGCAG	2 cut(s)	1602, 1692
Bsh1236I	CGCG	2 cut(s)	423, 2190
BshFI	GGCC	5 cut(s)	389, 754, 1883, 2230, 2570
BshNI	GGYRCC	2 cut(s)	4, 596
BshTI	ACCGGT	1 cut(s)	1801
BshVI	ATCGAT	2 cut(s)	137, 157
BsiHKAI	GWGCWC	3 cut(s)	261, 903, 2188
BsiSI	CCGG	6 cut(s)	185, 233, 288, 651, 808, 1802
BslFI	GGGAC	2 cut(s)	230, 912
BslI	CCNNNNNNNGG	7 cut(s)	184, 565, 1164, 1378, 2097, 2252, 2647
BsmAI	GTCTC	3 cut(s)	411, 2364, 2653
BsmFI	GGGAC	2 cut(s)	230, 912
BsmI	GAATGC	1 cut(s)	2260
BsnI	GGCC	5 cut(s)	389, 754, 1883, 2230, 2570
Bso31I	GGTCTC	1 cut(s)	411
Bsp119I	TTCGAA	1 cut(s)	2725
Bsp1286I	GDGCHC	4 cut(s)	261, 903, 1392, 2188
Bsp1407I	TGTACA	1 cut(s)	852
Bsp143I	GATC	8 cut(s)	138, 435, 493, 1001, 1426, 2038, 2440, 2581
Bsp1720I	GCTNAGC	1 cut(s)	192
Bsp68I	TCGCGA	1 cut(s)	2190
BspANI	GGCC	5 cut(s)	389, 754, 1883, 2230, 2570
BspCNI	CTCAG	7 cut(s)	205, 1222, 1369, 1483, 1708, 2298, 2477
BspDI	ATCGAT	2 cut(s)	137, 157
BspFNI	CGCG	2 cut(s)	423, 2190
BspLI	GGNNCC	8 cut(s)	6, 245, 246, 270, 598, 753, 928, 2121
BspMAI	CTGCAG	1 cut(s)	2542
BspT104I	TTCGAA	1 cut(s)	2725
BspT107I	GGYRCC	2 cut(s)	4, 596
BspTI	CTTAAG	1 cut(s)	892
BspTNI	GGTCTC	1 cut(s)	411
BsrBI	CCGCTC	1 cut(s)	2387
BsrDI	GCAATG	2 cut(s)	1481, 1531
BsrFI	RCCGGY	2 cut(s)	287, 1801
BsrGI	TGTACA	1 cut(s)	852
BsrI	ACTGG	4 cut(s)	208, 474, 1797, 2316
BssAI	RCCGGY	2 cut(s)	287, 1801
BssECI	CCNNGG	3 cut(s)	183, 214, 2547
BssMI	GATC	8 cut(s)	138, 435, 493, 1001, 1426, 2038, 2440, 2581
BssNAI	GTATAC	1 cut(s)	1227
BssNI	GRCGYC	2 cut(s)	2414, 2544
BssT1I	CCWWGG	1 cut(s)	2547
Bst1107I	GTATAC	1 cut(s)	1227
Bst2UI	CCWGG	2 cut(s)	216, 1338
Bst4CI	ACNGT	7 cut(s)	683, 697, 938, 1147, 1224, 2183, 2252
Bst6I	CTCTTC	1 cut(s)	2559
BstACI	GRCGYC	2 cut(s)	2414, 2544
BstAFI	CTTAAG	1 cut(s)	892
BstAPI	GCANNNNNTGC	2 cut(s)	470, 1625
BstAUI	TGTACA	1 cut(s)	852
BstBAI	YACGTR	6 cut(s)	580, 906, 1994, 2351, 2555, 2749
BstBI	TTCGAA	1 cut(s)	2725
BstC8I	GCNNGC	7 cut(s)	289, 377, 756, 1621, 2188, 2470, 2633
BstDEI	CTNAG	8 cut(s)	192, 1230, 1377, 1470, 1695, 1747, 2285, 2464
BstENI	CCTNNNNNAGG	1 cut(s)	1376
BstFNI	CGCG	2 cut(s)	423, 2190
BstH2I	RGCGCY	2 cut(s)	232, 777
BstHHI	GCGC	5 cut(s)	231, 425, 776, 2140, 2653
BstKTI	GATC	8 cut(s)	141, 438, 496, 1004, 1429, 2041, 2443, 2584
BstMAI	GTCTC	3 cut(s)	411, 2364, 2653
BstMBI	GATC	8 cut(s)	138, 435, 493, 1001, 1426, 2038, 2440, 2581
BstMWI	GCNNNNNNNGC	6 cut(s)	464, 470, 605, 1105, 1625, 1634
BstNI	CCWGG	2 cut(s)	216, 1338
BstNSI	RCATGY	2 cut(s)	859, 1598
BstSCI	CCNGG	4 cut(s)	183, 214, 231, 1336
BstSFI	CTRYAG	1 cut(s)	2538
BstSLI	GKGCMC	1 cut(s)	1392
BstUI	CGCG	2 cut(s)	423, 2190
BstV1I	GCAGC	6 cut(s)	476, 798, 1027, 2308, 2409, 2665
BstV2I	GAAGAC	1 cut(s)	494
BstX2I	RGATCY	1 cut(s)	2440
BstYI	RGATCY	1 cut(s)	2440
BstZ17I	GTATAC	1 cut(s)	1227
Bsu15I	ATCGAT	2 cut(s)	137, 157
BsuRI	GGCC	5 cut(s)	389, 754, 1883, 2230, 2570
BsuTUI	ATCGAT	2 cut(s)	137, 157
BtsI	GCAGTG	1 cut(s)	1912
BtsIMutI	CAGTG	8 cut(s)	481, 1011, 1046, 1703, 1912, 2188, 2257, 2323
BtuMI	TCGCGA	1 cut(s)	2190
Cac8I	GCNNGC	7 cut(s)	289, 377, 756, 1621, 2188, 2470, 2633
CfoI	GCGC	5 cut(s)	231, 425, 776, 2140, 2653
Cfr10I	RCCGGY	2 cut(s)	287, 1801
Cfr13I	GGNCC	5 cut(s)	244, 268, 752, 926, 2120
ClaI	ATCGAT	2 cut(s)	137, 157
CseI	GACGC	1 cut(s)	2552
Csp6I	GTAC	4 cut(s)	5, 853, 939, 2313
CspAI	ACCGGT	1 cut(s)	1801
CviAII	CATG	6 cut(s)	856, 920, 1532, 1595, 2166, 2572
CviQI	GTAC	4 cut(s)	5, 853, 939, 2313
DdeI	CTNAG	8 cut(s)	192, 1230, 1377, 1470, 1695, 1747, 2285, 2464
DpnI	GATC	8 cut(s)	140, 437, 495, 1003, 1428, 2040, 2442, 2583
DpnII	GATC	8 cut(s)	138, 435, 493, 1001, 1426, 2038, 2440, 2581
DraI	TTTAAA	1 cut(s)	691
Eam1104I	CTCTTC	1 cut(s)	2559
EarI	CTCTTC	1 cut(s)	2559
Ecl136II	GAGCTC	1 cut(s)	901
Eco130I	CCWWGG	1 cut(s)	2547
Eco24I	GRGCYC	1 cut(s)	903
Eco31I	GGTCTC	1 cut(s)	411
Eco32I	GATATC	2 cut(s)	454, 1516
Eco47I	GGWCC	4 cut(s)	244, 268, 926, 2120
Eco53kI	GAGCTC	1 cut(s)	901
Eco57I	CTGAAG	3 cut(s)	422, 639, 2293
Eco72I	CACGTG	2 cut(s)	1994, 2555
EcoICRI	GAGCTC	1 cut(s)	901
EcoNI	CCTNNNNNAGG	1 cut(s)	1376
EcoO109I	RGGNCCY	2 cut(s)	244, 2120
EcoRII	CCWGG	2 cut(s)	214, 1336
EcoRV	GATATC	2 cut(s)	454, 1516
EcoT14I	CCWWGG	1 cut(s)	2547
EcoT22I	ATGCAT	1 cut(s)	610
EcoT38I	GRGCYC	1 cut(s)	903
ErhI	CCWWGG	1 cut(s)	2547
FaeI	CATG	6 cut(s)	859, 923, 1535, 1598, 2169, 2575
FalI	AAGNNNNNCTT	8 cut(s)	1626, 1658, 1862, 1894, 1933, 1965, 2430, 2462
FaqI	GGGAC	2 cut(s)	230, 912
FatI	CATG	6 cut(s)	855, 919, 1531, 1594, 2165, 2571
FauI	CCCGC	2 cut(s)	98, 2465
FblI	GTMKAC	2 cut(s)	312, 1226
FriOI	GRGCYC	1 cut(s)	903
FspBI	CTAG	3 cut(s)	777, 2663, 2675
FspI	TGCGCA	1 cut(s)	2139
GlaI	GCGC	5 cut(s)	230, 424, 775, 2139, 2652
HaeII	RGCGCY	2 cut(s)	232, 777
HaeIII	GGCC	5 cut(s)	389, 754, 1883, 2230, 2570
HapII	CCGG	6 cut(s)	185, 233, 288, 651, 808, 1802
HgaI	GACGC	1 cut(s)	2552
HhaI	GCGC	5 cut(s)	231, 425, 776, 2140, 2653
Hin1I	GRCGYC	2 cut(s)	2414, 2544
Hin1II	CATG	6 cut(s)	859, 923, 1535, 1598, 2169, 2575
Hin6I	GCGC	5 cut(s)	229, 423, 774, 2138, 2651
HinP1I	GCGC	5 cut(s)	229, 423, 774, 2138, 2651
HincII	GTYRAC	3 cut(s)	1143, 1437, 1758
HindII	GTYRAC	3 cut(s)	1143, 1437, 1758
HindIII	AAGCTT	1 cut(s)	889
HinfI	GANTC	4 cut(s)	159, 362, 1306, 1351
HpaI	GTTAAC	1 cut(s)	1437
HpaII	CCGG	6 cut(s)	185, 233, 288, 651, 808, 1802
HphI	GGTGA	5 cut(s)	154, 1133, 1256, 2278, 2539
Hpy166II	GTNNAC	9 cut(s)	313, 723, 853, 1143, 1227, 1437, 1758, 2278, 2378
Hpy8I	GTNNAC	9 cut(s)	313, 723, 853, 1143, 1227, 1437, 1758, 2278, 2378
Hpy99I	CGWCG	3 cut(s)	117, 278, 2508
HpyAV	CCTTC	8 cut(s)	569, 614, 1169, 1304, 2186, 2190, 2241, 2518
HpyCH4III	ACNGT	7 cut(s)	683, 697, 938, 1147, 1224, 2183, 2252
HpyF10VI	GCNNNNNNNGC	6 cut(s)	464, 470, 605, 1105, 1625, 1634
HpyF3I	CTNAG	8 cut(s)	192, 1230, 1377, 1470, 1695, 1747, 2285, 2464
Hsp92I	GRCGYC	2 cut(s)	2414, 2544
Hsp92II	CATG	6 cut(s)	859, 923, 1535, 1598, 2169, 2575
HspAI	GCGC	5 cut(s)	229, 423, 774, 2138, 2651
KflI	GGGWCCC	1 cut(s)	244
KpnI	GGTACC	1 cut(s)	8
KroI	GCCGGC	1 cut(s)	287
KroNI	GCCGGC	1 cut(s)	289
KspAI	GTTAAC	1 cut(s)	1437
Kzo9I	GATC	8 cut(s)	138, 435, 493, 1001, 1426, 2038, 2440, 2581
LmnI	GCTCC	5 cut(s)	264, 623, 1048, 1306, 2384
Lsp1109I	GCAGC	6 cut(s)	476, 798, 1027, 2308, 2409, 2665
LweI	GCATC	8 cut(s)	286, 617, 904, 1066, 1228, 1276, 1681, 2125
MaeI	CTAG	3 cut(s)	777, 2663, 2675
MaeIII	GTNAC	5 cut(s)	715, 787, 1597, 1846, 2161
MalI	GATC	8 cut(s)	140, 437, 495, 1003, 1428, 2040, 2442, 2583
MbiI	CCGCTC	1 cut(s)	2387
MboI	GATC	8 cut(s)	138, 435, 493, 1001, 1426, 2038, 2440, 2581
MfeI	CAATTG	1 cut(s)	263
MflI	RGATCY	1 cut(s)	2440
MhlI	GDGCHC	4 cut(s)	261, 903, 1392, 2188
MmeI	TCCRAC	2 cut(s)	100, 636
Mph1103I	ATGCAT	1 cut(s)	610
MroNI	GCCGGC	1 cut(s)	287
MroXI	GAANNNNTTC	3 cut(s)	663, 803, 2442
MslI	CAYNNNNRTG	3 cut(s)	530, 1272, 1769
MspA1I	CMGCKG	1 cut(s)	2468
MspCI	CTTAAG	1 cut(s)	892
MspI	CCGG	6 cut(s)	185, 233, 288, 651, 808, 1802
MspR9I	CCNGG	4 cut(s)	185, 216, 233, 1338
MunI	CAATTG	1 cut(s)	263
Mva1269I	GAATGC	1 cut(s)	2260
MvaI	CCWGG	2 cut(s)	216, 1338
MvnI	CGCG	2 cut(s)	423, 2190
MwoI	GCNNNNNNNGC	6 cut(s)	464, 470, 605, 1105, 1625, 1634
NaeI	GCCGGC	1 cut(s)	289
NciI	CCSGG	2 cut(s)	185, 233
NdeII	GATC	8 cut(s)	138, 435, 493, 1001, 1426, 2038, 2440, 2581
NgoMIV	GCCGGC	1 cut(s)	287
NlaIII	CATG	6 cut(s)	859, 923, 1535, 1598, 2169, 2575
NlaIV	GGNNCC	8 cut(s)	6, 245, 246, 270, 598, 753, 928, 2121
NmeAIII	GCCGAG	1 cut(s)	225
NmuCI	GTSAC	3 cut(s)	715, 1846, 2161
NruI	TCGCGA	1 cut(s)	2190
NsbI	TGCGCA	1 cut(s)	2139
NsiI	ATGCAT	1 cut(s)	610
NspI	RCATGY	2 cut(s)	859, 1598
NspV	TTCGAA	1 cut(s)	2725
PciI	ACATGT	1 cut(s)	1594
PcsI	WCGNNNNNNNCGW	1 cut(s)	2622
PctI	GAATGC	1 cut(s)	2260
PdiI	GCCGGC	1 cut(s)	289
PdmI	GAANNNNTTC	3 cut(s)	663, 803, 2442
PfeI	GAWTC	4 cut(s)	159, 362, 1306, 1351
PflFI	GACNNNGTC	1 cut(s)	278
PflMI	CCANNNNNTGG	1 cut(s)	2252
PinAI	ACCGGT	1 cut(s)	1801
PmaCI	CACGTG	2 cut(s)	1994, 2555
PmlI	CACGTG	2 cut(s)	1994, 2555
Ppu21I	YACGTR	6 cut(s)	580, 906, 1994, 2351, 2555, 2749
PpuMI	RGGWCCY	2 cut(s)	244, 2120
PscI	ACATGT	1 cut(s)	1594
PshBI	ATTAAT	1 cut(s)	1152
PsiI	TTATAA	1 cut(s)	732
Psp124BI	GAGCTC	1 cut(s)	903
Psp1406I	AACGTT	3 cut(s)	1323, 2224, 2625
Psp5II	RGGWCCY	2 cut(s)	244, 2120
Psp6I	CCWGG	2 cut(s)	214, 1336
PspCI	CACGTG	2 cut(s)	1994, 2555
PspGI	CCWGG	2 cut(s)	214, 1336
PspN4I	GGNNCC	8 cut(s)	6, 245, 246, 270, 598, 753, 928, 2121
PspPI	GGNCC	5 cut(s)	244, 268, 752, 926, 2120
PspPPI	RGGWCCY	2 cut(s)	244, 2120
PstI	CTGCAG	1 cut(s)	2542
PsuI	RGATCY	1 cut(s)	2440
PsyI	GACNNNGTC	1 cut(s)	278
PvuII	CAGCTG	1 cut(s)	2468
RruI	TCGCGA	1 cut(s)	2190
RsaI	GTAC	4 cut(s)	6, 854, 940, 2314
RsaNI	GTAC	4 cut(s)	5, 853, 939, 2313
RseI	CAYNNNNRTG	3 cut(s)	530, 1272, 1769
SacI	GAGCTC	1 cut(s)	903
Sau3AI	GATC	8 cut(s)	138, 435, 493, 1001, 1426, 2038, 2440, 2581
Sau96I	GGNCC	5 cut(s)	244, 268, 752, 926, 2120
ScrFI	CCNGG	4 cut(s)	185, 216, 233, 1338
SduI	GDGCHC	4 cut(s)	261, 903, 1392, 2188
SfaNI	GCATC	8 cut(s)	286, 617, 904, 1066, 1228, 1276, 1681, 2125
SfcI	CTRYAG	1 cut(s)	2538
SfuI	TTCGAA	1 cut(s)	2725
SinI	GGWCC	4 cut(s)	244, 268, 926, 2120
SmiMI	CAYNNNNRTG	3 cut(s)	530, 1272, 1769
SmlI	CTYRAG	4 cut(s)	892, 1538, 1724, 2095
SmoI	CTYRAG	4 cut(s)	892, 1538, 1724, 2095
SspI	AATATT	2 cut(s)	664, 711
SspMI	CTAG	3 cut(s)	777, 2663, 2675
SstI	GAGCTC	1 cut(s)	903
StyD4I	CCNGG	4 cut(s)	183, 214, 231, 1336
StyI	CCWWGG	1 cut(s)	2547
TaaI	ACNGT	7 cut(s)	683, 697, 938, 1147, 1224, 2183, 2252
TaqI	TCGA	8 cut(s)	137, 157, 326, 450, 933, 1560, 1682, 2725
TatI	WGTACW	2 cut(s)	852, 938
TauI	GCSGC	7 cut(s)	199, 372, 375, 392, 461, 464, 2390
TfiI	GAWTC	4 cut(s)	159, 362, 1306, 1351
TscAI	CASTG	8 cut(s)	481, 1011, 1053, 1703, 1912, 2188, 2257, 2323
TseFI	GTSAC	3 cut(s)	715, 1846, 2161
TseI	GCWGC	6 cut(s)	464, 811, 1040, 2321, 2422, 2653
Tsp45I	GTSAC	3 cut(s)	715, 1846, 2161
TspRI	CASTG	8 cut(s)	481, 1011, 1053, 1703, 1912, 2188, 2257, 2323
Tth111I	GACNNNGTC	1 cut(s)	278
Van91I	CCANNNNNTGG	1 cut(s)	2252
Vha464I	CTTAAG	1 cut(s)	892
VpaK11BI	GGWCC	4 cut(s)	244, 268, 926, 2120
VspI	ATTAAT	1 cut(s)	1152
XagI	CCTNNNNNAGG	1 cut(s)	1376
XapI	RAATTY	5 cut(s)	1124, 1382, 1729, 1962, 2492
XceI	RCATGY	2 cut(s)	859, 1598
XcmI	CCANNNNNNNNNTGG	2 cut(s)	215, 1876
XmiI	GTMKAC	2 cut(s)	312, 1226
XmnI	GAANNNNTTC	3 cut(s)	663, 803, 2442
XspI	CTAG	3 cut(s)	777, 2663, 2675
ZraI	GACGTC	1 cut(s)	2415
Zsp2I	ATGCAT	1 cut(s)	610

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.