MD12G1014300.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003806
Physical Location & Seq
Forward (+)
1482472 .. 1483686
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1014300.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGGAGAAGAGATTAAAAAGAGTCTCTTGGCAAAAGAGACTAGTGTTGGAGAAGAAAAGACTAGAGTTATTGATGGCAATGAGGAATTGTCATTGAAAAGAAAGGTATGGATTGAAACCAAGAAGATATGGGTGGTGGCTGGCCCTGCAACATTCACCAGAGTTGCGACTTTTGGGACAAATGTCCTCAGTCAAGCGTTTATCGGCCACATTGGCTCTACGCAGTTGGTGGCTTTTTCACTCTTGTTCACCGTACTTGTCAGGTTCGCAAACGGCATCTTGCTTGGCATGGCAAGTGCGCTGGGAACTCTCTGCGGTCAATCGTATGGTGCAAAACAGTACCACATGCTCGGGATACATCTTCAAAGGTCATGGATAGTAGTGTTTGTAGGCACATTATTCCTCATTCCTCTTTACGTTTTTACAACCCCCATTTTAGAGGCTTTAGGCCAAGAAGAAAATATTTCAAAATTTGCAGGATATATTTCTCTATGGGTAATCCCTGTGCTTTTTGCATTTGTGGTCTCATTTACCTGCCAAATGTACTTACAAACGCAAAGCAAAACTATAATCGTTGCATATGTGTCAGCGATTTCAATTGGAATCCACATCTTTCTTTGCTGGGTTTTGAGTGTGAAATTTAAGTTTGGGGTTCCCGGTGTAATGGTATCAACGCTTATATCATATTGGTTACCCAACCTGGGTCAGCTTTTGTTTATTCTACGTGGAGGATGTGTTGTGCGGAAGCGTCAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

253

Amino Acids

27.93

Weight (kDa)

9.58

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 49 - 209 3e-26 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 542
AciI CCGC 2 cut(s) 315, 742
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 2 cut(s) 470, 638
AfaI GTAC 3 cut(s) 255, 341, 545
AfiI CCNNNNNNNGG 1 cut(s) 701
AgsI TTSAA 5 cut(s) 97, 116, 365, 468, 597
AhlI ACTAGT 1 cut(s) 41
AjnI CCWGG 1 cut(s) 699
AluBI AGCT 1 cut(s) 709
AluI AGCT 1 cut(s) 709
Alw26I GTCTC 3 cut(s) 29, 32, 529
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 3 cut(s) 142, 205, 448
ApoI RAATTY 2 cut(s) 470, 638
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 1 cut(s) 657
AsuHPI GGTGA 2 cut(s) 148, 241
AvaI CYCGRG 1 cut(s) 350
BccI CCATC 1 cut(s) 68
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 1 cut(s) 701
BciVI GTATCC 1 cut(s) 348
BcnI CCSGG 1 cut(s) 657
BcoDI GTCTC 3 cut(s) 29, 32, 529
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 2 cut(s) 42, 63
BfuAI ACCTGC 1 cut(s) 542
BfuI GTATCC 1 cut(s) 348
BglI GCCNNNNNGGC 1 cut(s) 213
Bme1390I CCNGG 2 cut(s) 657, 701
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 1 cut(s) 654
BmrFI CCNGG 2 cut(s) 657, 701
BmsI GCATC 1 cut(s) 285
BoxI GACNNNNGTC 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 657
BsaAI YACGTR 1 cut(s) 725
BsaI GGTCTC 1 cut(s) 529
BsaJI CCNNGG 1 cut(s) 700
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 1 cut(s) 701
Bse3DI GCAATG 1 cut(s) 85
BseBI CCWGG 1 cut(s) 701
BseDI CCNNGG 1 cut(s) 700
BseGI GGATG 1 cut(s) 737
BseLI CCNNNNNNNGG 1 cut(s) 701
BseMI GCAATG 1 cut(s) 85
BseMII CTCAG 1 cut(s) 202
BseYI CCCAGC 2 cut(s) 301, 621
BshFI GGCC 3 cut(s) 144, 207, 450
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 1 cut(s) 657
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 1 cut(s) 701
BsmAI GTCTC 3 cut(s) 29, 32, 529
BsmFI GGGAC 1 cut(s) 190
BsnI GGCC 3 cut(s) 144, 207, 450
Bso31I GGTCTC 1 cut(s) 529
BsoBI CYCGRG 1 cut(s) 350
BspACI CCGC 2 cut(s) 315, 742
BspANI GGCC 3 cut(s) 144, 207, 450
BspCNI CTCAG 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 654
BspMI ACCTGC 1 cut(s) 542
BspTNI GGTCTC 1 cut(s) 529
BsrDI GCAATG 1 cut(s) 85
BssECI CCNNGG 1 cut(s) 700
Bst2UI CCWGG 1 cut(s) 701
Bst4CI ACNGT 2 cut(s) 253, 339
Bst6I CTCTTC 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 725
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 1 cut(s) 188
BstEII GGTNACC 1 cut(s) 690
BstF5I GGATG 1 cut(s) 737
BstHHI GCGC 1 cut(s) 301
BstMAI GTCTC 3 cut(s) 29, 32, 529
BstMWI GCNNNNNNNGC 2 cut(s) 146, 213
BstNI CCWGG 1 cut(s) 701
BstNSI RCATGY 1 cut(s) 349
BstPAI GACNNNNGTC 1 cut(s) 182
BstPI GGTNACC 1 cut(s) 690
BstSCI CCNGG 2 cut(s) 655, 699
BsuI GTATCC 1 cut(s) 348
BsuRI GGCC 3 cut(s) 144, 207, 450
BtsCI GGATG 1 cut(s) 737
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 1 cut(s) 142
CfoI GCGC 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 143
CseI GACGC 1 cut(s) 737
Csp6I GTAC 3 cut(s) 254, 340, 544
CviAII CATG 3 cut(s) 289, 346, 372
CviJI RGCY 8 cut(s) 140, 144, 207, 216, 233, 443, 450, 709
CviKI_1 RGCY 8 cut(s) 140, 144, 207, 216, 233, 443, 450, 709
CviQI GTAC 3 cut(s) 254, 340, 544
DdeI CTNAG 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 529
Eco88I CYCGRG 1 cut(s) 350
Eco91I GGTNACC 1 cut(s) 690
EcoO65I GGTNACC 1 cut(s) 690
EcoRII CCWGG 1 cut(s) 699
FaeI CATG 3 cut(s) 292, 349, 375
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 1 cut(s) 190
FatI CATG 3 cut(s) 288, 345, 371
FauNDI CATATG 1 cut(s) 580
FokI GGATG 1 cut(s) 744
FspBI CTAG 2 cut(s) 42, 63
GlaI GCGC 1 cut(s) 300
GsaI CCCAGC 2 cut(s) 305, 625
HaeIII GGCC 3 cut(s) 144, 207, 450
HapII CCGG 1 cut(s) 657
HgaI GACGC 1 cut(s) 737
HhaI GCGC 1 cut(s) 301
Hin1II CATG 3 cut(s) 292, 349, 375
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HinfI GANTC 2 cut(s) 22, 603
HpaII CCGG 1 cut(s) 657
HphI GGTGA 2 cut(s) 148, 241
Hpy166II GTNNAC 1 cut(s) 249
Hpy188III TCNNGA 1 cut(s) 352
Hpy8I GTNNAC 1 cut(s) 249
HpyCH4III ACNGT 2 cut(s) 253, 339
HpyCH4IV ACGT 2 cut(s) 417, 724
HpyCH4V TGCA 5 cut(s) 149, 332, 476, 515, 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 213
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 2 cut(s) 417, 724
Hsp92II CATG 3 cut(s) 292, 349, 375
HspAI GCGC 1 cut(s) 299
LweI GCATC 1 cut(s) 285
MaeI CTAG 2 cut(s) 42, 63
MaeII ACGT 2 cut(s) 417, 724
MaeIII GTNAC 1 cut(s) 690
MboII GAAGA 5 cut(s) 20, 65, 136, 353, 467
MfeI CAATTG 1 cut(s) 597
MluCI AATT 4 cut(s) 86, 470, 597, 638
MlyI GAGTC 1 cut(s) 31
MmeI TCCRAC 1 cut(s) 28
MnlI CCTC 6 cut(s) 76, 197, 413, 420, 433, 722
MseI TTAA 2 cut(s) 15, 642
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 2 cut(s) 657, 701
MunI CAATTG 1 cut(s) 597
MvaI CCWGG 1 cut(s) 701
MwoI GCNNNNNNNGC 2 cut(s) 146, 213
NciI CCSGG 1 cut(s) 657
NdeI CATATG 1 cut(s) 580
NlaIII CATG 3 cut(s) 292, 349, 375
NlaIV GGNNCC 1 cut(s) 654
NspI RCATGY 1 cut(s) 349
PfeI GAWTC 1 cut(s) 603
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
Ppu21I YACGTR 1 cut(s) 725
PshAI GACNNNNGTC 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 699
PspEI GGTNACC 1 cut(s) 690
PspFI CCCAGC 2 cut(s) 301, 621
PspGI CCWGG 1 cut(s) 699
PspN4I GGNNCC 1 cut(s) 654
PspPI GGNCC 1 cut(s) 143
RsaI GTAC 3 cut(s) 255, 341, 545
RsaNI GTAC 3 cut(s) 254, 340, 544
SaqAI TTAA 2 cut(s) 15, 642
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 2 cut(s) 657, 701
SetI ASST 8 cut(s) 108, 266, 371, 420, 536, 702, 711, 727
SfaNI GCATC 1 cut(s) 285
SpeI ACTAGT 1 cut(s) 41
Sse9I AATT 4 cut(s) 86, 470, 597, 638
SsiI CCGC 2 cut(s) 315, 742
SspI AATATT 1 cut(s) 463
SspMI CTAG 2 cut(s) 42, 63
StyD4I CCNGG 2 cut(s) 655, 699
TaaI ACNGT 2 cut(s) 253, 339
TaiI ACGT 2 cut(s) 420, 727
TasI AATT 4 cut(s) 86, 470, 597, 638
TatI WGTACW 1 cut(s) 543
TfiI GAWTC 1 cut(s) 603
Tru1I TTAA 2 cut(s) 15, 642
Tru9I TTAA 2 cut(s) 15, 642
XapI RAATTY 2 cut(s) 470, 638
XceI RCATGY 1 cut(s) 349
XspI CTAG 2 cut(s) 42, 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.