Rroxscaffold_6G00390260

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003806
Physical Location & Seq
Forward (+)
4998855 .. 5001004
2150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00390260.1

Sequence Viewer

Length: 777 bp
ATGATCATTGTTTACTTGTCAGCATTTGCTCTTGCAGTCCATATCCTCCTTTCATGGCTTTTAACTGTGAGATTTAAATTTGGGATACCTGGTGCAATGCTGTCAACAATTGTAGCATTTTGGATACCAAATTTTGGTCAGCTTTTATTTATTACATGTGGAGGATGTCCAGAAACGTGGAAAGGTTTCTCCACCTTAGCTTTCAAAGATCTCTGGGATATTATCAAACTTTCTCTGTCTTCCGGTGTTATGCTTTGGTCTTATAATATACTTATTTTGGTCGTTGAGAAAATCTGGATACAGCCTCAACATCAATGGGTGGGAAATGATGATATCCCTGGGCTTCTTAGCGGCTGCAAGTTTGGGTGTCGAATTTATAGTGTACTAGTGTCAAATGAACTTGGAAGAGGTAGCTCAGGAGGGGCAAAGTTCTCAATCGTGGTTATAGTGATCACATCATTTGCCATTGGTTTTGTGCTTTTCTTGTTCTTCCTGTTCTTAAGGGAGCGTCTTGCCTACATTTTCACTGACAATGAAGAGGTAGCTGAAGCCTTCACCGATTTGTCACCTCTATTGGCTTTCTCCATATTGCTGAACAGTGTTCAACCTGTGCTCTCTGGGTGTTTGGATTGGAATGTTGTTTGGACATTTGTGCAGACTATTGTACTAGTCATATTTACCTACAAAACTGATTGGGATAAACAGGTATCCATAGCTCGTAATCGTGTTAACAACTGGGTGGTAACTGACAATCCGGAATCGAGCCCCGACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

258

Amino Acids

28.98

Weight (kDa)

5.36

Isoelectric Point (pI)

24.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 127 - 210 1.1e-11 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 264
AccB7I CCANNNNNTGG 1 cut(s) 134
AccIII TCCGGA 1 cut(s) 754
AciI CCGC 1 cut(s) 351
AcsI RAATTY 3 cut(s) 77, 130, 372
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 2 cut(s) 384, 666
AfiI CCNNNNNNNGG 1 cut(s) 134
AflII CTTAAG 1 cut(s) 499
AflIII ACRYGT 1 cut(s) 155
AgsI TTSAA 2 cut(s) 205, 605
AhlI ACTAGT 2 cut(s) 385, 667
AjnI CCWGG 2 cut(s) 88, 337
AluBI AGCT 5 cut(s) 142, 200, 414, 545, 716
AluI AGCT 5 cut(s) 142, 200, 414, 545, 716
Alw21I GWGCWC 1 cut(s) 615
Aor13HI TCCGGA 1 cut(s) 754
ApeKI GCWGC 1 cut(s) 354
ApoI RAATTY 3 cut(s) 77, 130, 372
Asp700I GAANNNNTTC 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 547, 558
BanII GRGCYC 1 cut(s) 767
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 615
BbvI GCAGC 1 cut(s) 341
BciT130I CCWGG 2 cut(s) 90, 339
BciVI GTATCC 4 cut(s) 78, 117, 291, 718
BclI TGATCA 2 cut(s) 3, 450
BcuI ACTAGT 2 cut(s) 385, 667
BfaI CTAG 2 cut(s) 386, 668
BfrI CTTAAG 1 cut(s) 499
BfuI GTATCC 4 cut(s) 78, 117, 291, 718
BglII AGATCT 1 cut(s) 208
BisI GCNGC 2 cut(s) 352, 355
BlsI GCNGC 2 cut(s) 353, 356
Bme1390I CCNGG 2 cut(s) 90, 339
BmrFI CCNGG 2 cut(s) 90, 339
BmrI ACTGGG 1 cut(s) 745
BmuI ACTGGG 1 cut(s) 745
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 2 cut(s) 196, 415
BsaJI CCNNGG 2 cut(s) 337, 338
BsaWI WCCGGW 2 cut(s) 242, 754
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 740
Bse3DI GCAATG 1 cut(s) 102
BseAI TCCGGA 1 cut(s) 754
BseBI CCWGG 2 cut(s) 90, 339
BseDI CCNNGG 2 cut(s) 337, 338
BseGI GGATG 1 cut(s) 170
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 1 cut(s) 740
BseXI GCAGC 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 615
BsiSI CCGG 2 cut(s) 243, 755
BslI CCNNNNNNNGG 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 615, 767
Bsp13I TCCGGA 1 cut(s) 754
Bsp143I GATC 3 cut(s) 3, 208, 450
BspACI CCGC 1 cut(s) 351
BspCNI CTCAG 1 cut(s) 428
BspEI TCCGGA 1 cut(s) 754
BspTI CTTAAG 1 cut(s) 499
BsrDI GCAATG 1 cut(s) 102
BsrI ACTGG 1 cut(s) 740
BssECI CCNNGG 2 cut(s) 337, 338
BssMI GATC 3 cut(s) 3, 208, 450
Bst2UI CCWGG 2 cut(s) 90, 339
Bst4CI ACNGT 2 cut(s) 67, 599
Bst6I CTCTTC 2 cut(s) 400, 531
BstAFI CTTAAG 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 196, 347, 415
BstF5I GGATG 1 cut(s) 170
BstKTI GATC 3 cut(s) 6, 211, 453
BstMBI GATC 3 cut(s) 3, 208, 450
BstNI CCWGG 2 cut(s) 90, 339
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 2 cut(s) 88, 337
BstV1I GCAGC 1 cut(s) 341
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 208
BstXI CCANNNNNNTGG 1 cut(s) 177
BstYI RGATCY 1 cut(s) 208
BsuI GTATCC 4 cut(s) 78, 117, 291, 718
BtsCI GGATG 1 cut(s) 170
BtsIMutI CAGTG 2 cut(s) 525, 604
CseI GACGC 1 cut(s) 497
CsiI ACCWGGT 1 cut(s) 88
Csp6I GTAC 2 cut(s) 383, 665
CviAII CATG 2 cut(s) 54, 156
CviQI GTAC 2 cut(s) 383, 665
DdeI CTNAG 3 cut(s) 196, 347, 415
DpnI GATC 3 cut(s) 5, 210, 452
DpnII GATC 3 cut(s) 3, 208, 450
DraI TTTAAA 1 cut(s) 76
Eam1104I CTCTTC 2 cut(s) 400, 531
EarI CTCTTC 2 cut(s) 400, 531
Eco24I GRGCYC 1 cut(s) 767
Eco32I GATATC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 567
EcoRII CCWGG 2 cut(s) 88, 337
EcoRV GATATC 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 767
FaeI CATG 2 cut(s) 57, 159
FatI CATG 2 cut(s) 53, 155
FbaI TGATCA 2 cut(s) 3, 450
Fnu4HI GCNGC 2 cut(s) 352, 355
FokI GGATG 1 cut(s) 177
FriOI GRGCYC 1 cut(s) 767
Fsp4HI GCNGC 2 cut(s) 352, 355
FspBI CTAG 2 cut(s) 386, 668
GluI GCNGC 2 cut(s) 352, 355
HapII CCGG 2 cut(s) 243, 755
HgaI GACGC 1 cut(s) 497
Hin1II CATG 2 cut(s) 57, 159
HincII GTYRAC 2 cut(s) 105, 730
HindII GTYRAC 2 cut(s) 105, 730
HinfI GANTC 1 cut(s) 758
HpaI GTTAAC 1 cut(s) 730
HpaII CCGG 2 cut(s) 243, 755
HphI GGTGA 2 cut(s) 547, 558
Hpy166II GTNNAC 4 cut(s) 13, 105, 383, 730
Hpy188III TCNNGA 4 cut(s) 170, 295, 417, 755
Hpy8I GTNNAC 4 cut(s) 13, 105, 383, 730
HpyAV CCTTC 1 cut(s) 562
HpyCH4III ACNGT 2 cut(s) 67, 599
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 4 cut(s) 35, 95, 357, 655
HpyF3I CTNAG 3 cut(s) 196, 347, 415
HpySE526I ACGT 1 cut(s) 176
Hsp92II CATG 2 cut(s) 57, 159
Kpn2I TCCGGA 1 cut(s) 754
Ksp22I TGATCA 2 cut(s) 3, 450
KspAI GTTAAC 1 cut(s) 730
Kzo9I GATC 3 cut(s) 3, 208, 450
LmnI GCTCC 1 cut(s) 505
Lsp1109I GCAGC 1 cut(s) 341
MabI ACCWGGT 1 cut(s) 88
MaeI CTAG 2 cut(s) 386, 668
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 2 cut(s) 564, 742
MalI GATC 3 cut(s) 5, 210, 452
MboI GATC 3 cut(s) 3, 208, 450
MboII GAAGA 4 cut(s) 231, 417, 481, 548
MfeI CAATTG 1 cut(s) 108
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 2 cut(s) 615, 767
MluCI AATT 4 cut(s) 77, 108, 130, 372
MnlI CCTC 7 cut(s) 56, 155, 315, 401, 413, 532, 579
MroI TCCGGA 1 cut(s) 754
MroXI GAANNNNTTC 1 cut(s) 185
MseI TTAA 4 cut(s) 62, 75, 500, 729
MspCI CTTAAG 1 cut(s) 499
MspI CCGG 2 cut(s) 243, 755
MspR9I CCNGG 2 cut(s) 90, 339
MunI CAATTG 1 cut(s) 108
MvaI CCWGG 2 cut(s) 90, 339
NdeII GATC 3 cut(s) 3, 208, 450
NlaIII CATG 2 cut(s) 57, 159
NmuCI GTSAC 1 cut(s) 564
NspI RCATGY 1 cut(s) 159
PasI CCCWGGG 1 cut(s) 338
PciI ACATGT 1 cut(s) 155
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 1 cut(s) 758
PflMI CCANNNNNTGG 1 cut(s) 134
PkrI GCNGC 2 cut(s) 353, 356
PscI ACATGT 1 cut(s) 155
PsiI TTATAA 1 cut(s) 264
Psp6I CCWGG 2 cut(s) 88, 337
PspGI CCWGG 2 cut(s) 88, 337
PsuI RGATCY 1 cut(s) 208
RsaI GTAC 2 cut(s) 384, 666
RsaNI GTAC 2 cut(s) 383, 665
SaqAI TTAA 4 cut(s) 62, 75, 500, 729
SatI GCNGC 2 cut(s) 352, 355
Sau3AI GATC 3 cut(s) 3, 208, 450
ScrFI CCNGG 2 cut(s) 90, 339
SduI GDGCHC 2 cut(s) 615, 767
SexAI ACCWGGT 1 cut(s) 88
SmiI ATTTAAAT 1 cut(s) 76
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
SpeI ACTAGT 2 cut(s) 385, 667
Sse9I AATT 4 cut(s) 77, 108, 130, 372
SsiI CCGC 1 cut(s) 351
SspMI CTAG 2 cut(s) 386, 668
StyD4I CCNGG 2 cut(s) 88, 337
SwaI ATTTAAAT 1 cut(s) 76
TaaI ACNGT 2 cut(s) 67, 599
TaiI ACGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 370, 761
TasI AATT 4 cut(s) 77, 108, 130, 372
TatI WGTACW 2 cut(s) 382, 664
TauI GCSGC 1 cut(s) 354
TfiI GAWTC 1 cut(s) 758
Tru1I TTAA 4 cut(s) 62, 75, 500, 729
Tru9I TTAA 4 cut(s) 62, 75, 500, 729
TscAI CASTG 2 cut(s) 532, 604
TseFI GTSAC 1 cut(s) 564
TseI GCWGC 1 cut(s) 354
Tsp45I GTSAC 1 cut(s) 564
TspDTI ATGAA 3 cut(s) 42, 411, 549
TspRI CASTG 2 cut(s) 532, 604
Van91I CCANNNNNTGG 1 cut(s) 134
Vha464I CTTAAG 1 cut(s) 499
XapI RAATTY 3 cut(s) 77, 130, 372
XceI RCATGY 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 185
XspI CTAG 2 cut(s) 386, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.