Rorug03G0274600

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003806
Physical Location & Seq
Forward (+)
27143394 .. 27143762
369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0274600.1

Sequence Viewer

Length: 369 bp
ATGCCGTTATTGGTGGAATTAGGTAGGACACCCGGAGTGTCGAAACAGCGCTGGAGGAAAGGTTTCAAATTTGAGGAATTTTGCGTTAGACATGCAAAGTGTGATGAGGTGGTAACAAAGGGGTGGTCTTTACCTGCTACGGATGACCCTATGCGTCACGTCTATTTGAAAATCAAAGCTACAAGCCATTTCTTACAGACTTGGCAGCAGCGTATTTTTCAACATCGGAAAGAGGAGTTGCAAGTGGTGCAGCAACGATTACAAACATTACTCAGTGCACCTTTCTTTGAGGCCCATGTTGAAGAGAAAAAAACTCTCGCTGCTTGGTATCATGAATTACAAACAATGGAGGATATGTATTGGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

122

Amino Acids

14.72

Weight (kDa)

9.2

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 142
AcsI RAATTY 2 cut(s) 68, 77
AfeI AGCGCT 1 cut(s) 50
AgsI TTSAA 4 cut(s) 67, 169, 221, 302
AjiI CACGTC 1 cut(s) 160
AluBI AGCT 1 cut(s) 179
AluI AGCT 1 cut(s) 179
Alw21I GWGCWC 1 cut(s) 280
Alw44I GTGCAC 1 cut(s) 276
Aor51HI AGCGCT 1 cut(s) 50
AoxI GGCC 1 cut(s) 291
ApaLI GTGCAC 1 cut(s) 276
ApeKI GCWGC 4 cut(s) 205, 208, 250, 320
ApoI RAATTY 2 cut(s) 68, 77
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
Asp700I GAANNNNTTC 1 cut(s) 62
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 1 cut(s) 292
AsuC2I CCSGG 1 cut(s) 33
BaeGI GKGCMC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 4 cut(s) 217, 220, 262, 307
BcnI CCSGG 1 cut(s) 33
BfoI RGCGCY 1 cut(s) 52
BfuAI ACCTGC 1 cut(s) 142
BisI GCNGC 4 cut(s) 206, 209, 251, 321
BlsI GCNGC 4 cut(s) 207, 210, 252, 322
Bme1390I CCNGG 1 cut(s) 33
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 292
BmrFI CCNGG 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 33
BsaXI ACNNNNNCTCC 3 cut(s) 46, 76, 341
BseGI GGATG 1 cut(s) 148
BseMII CTCAG 1 cut(s) 286
BseRI GAGGAG 1 cut(s) 248
BseSI GKGCMC 1 cut(s) 280
BseXI GCAGC 4 cut(s) 217, 220, 262, 307
BsgI GTGCAG 1 cut(s) 269
BshFI GGCC 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 33
BsnI GGCC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 280
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 1 cut(s) 285
BspHI TCATGA 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 247
BstDEI CTNAG 1 cut(s) 272
BstF5I GGATG 1 cut(s) 148
BstH2I RGCGCY 1 cut(s) 52
BstHHI GCGC 1 cut(s) 51
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNSI RCATGY 1 cut(s) 95
BstSCI CCNGG 1 cut(s) 31
BstSLI GKGCMC 1 cut(s) 280
BstV1I GCAGC 4 cut(s) 217, 220, 262, 307
BsuRI GGCC 1 cut(s) 293
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 280
BveI ACCTGC 1 cut(s) 142
CciI TCATGA 1 cut(s) 331
CfoI GCGC 1 cut(s) 51
Cfr13I GGNCC 1 cut(s) 292
CseI GACGC 1 cut(s) 143
CviAII CATG 3 cut(s) 92, 296, 332
CviJI RGCY 3 cut(s) 179, 186, 293
CviKI_1 RGCY 3 cut(s) 179, 186, 293
DdeI CTNAG 1 cut(s) 272
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco47III AGCGCT 1 cut(s) 50
FaeI CATG 3 cut(s) 95, 299, 335
FaiI YATR 5 cut(s) 93, 152, 297, 333, 356
FatI CATG 3 cut(s) 91, 295, 331
Fnu4HI GCNGC 4 cut(s) 206, 209, 251, 321
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 4 cut(s) 206, 209, 251, 321
GlaI GCGC 1 cut(s) 50
GluI GCNGC 4 cut(s) 206, 209, 251, 321
GsuI CTGGAG 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 52
HaeIII GGCC 1 cut(s) 293
HapII CCGG 1 cut(s) 33
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 51
Hin1II CATG 3 cut(s) 95, 299, 335
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HpaII CCGG 1 cut(s) 33
Hpy166II GTNNAC 1 cut(s) 278
Hpy188I TCNGA 1 cut(s) 228
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 1 cut(s) 278
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 4 cut(s) 95, 241, 250, 278
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 1 cut(s) 272
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 3 cut(s) 95, 299, 335
HspAI GCGC 1 cut(s) 49
LpnPI CCDG 3 cut(s) 37, 46, 147
Lsp1109I GCAGC 4 cut(s) 217, 220, 262, 307
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 2 cut(s) 112, 155
MboII GAAGA 1 cut(s) 314
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 4 cut(s) 17, 68, 77, 335
MnlI CCTC 7 cut(s) 48, 67, 100, 226, 283, 343, 357
MroXI GAANNNNTTC 1 cut(s) 62
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 247
NciI CCSGG 1 cut(s) 33
NlaIII CATG 3 cut(s) 95, 299, 335
NmuCI GTSAC 1 cut(s) 155
NspI RCATGY 1 cut(s) 95
PagI TCATGA 1 cut(s) 331
PdmI GAANNNNTTC 1 cut(s) 62
PkrI GCNGC 4 cut(s) 207, 210, 252, 322
PspPI GGNCC 1 cut(s) 292
SatI GCNGC 4 cut(s) 206, 209, 251, 321
Sau96I GGNCC 1 cut(s) 292
ScrFI CCNGG 1 cut(s) 33
SduI GDGCHC 1 cut(s) 280
SetI ASST 8 cut(s) 25, 64, 111, 136, 162, 181, 283, 368
Sse9I AATT 4 cut(s) 17, 68, 77, 335
StyD4I CCNGG 1 cut(s) 31
TaiI ACGT 1 cut(s) 162
TaqI TCGA 1 cut(s) 41
TasI AATT 4 cut(s) 17, 68, 77, 335
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 4 cut(s) 205, 208, 250, 320
Tsp45I GTSAC 1 cut(s) 155
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 1 cut(s) 280
VneI GTGCAC 1 cut(s) 276
XapI RAATTY 2 cut(s) 68, 77
XceI RCATGY 1 cut(s) 95
XmnI GAANNNNTTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.