MD12G1017600.v1.1

Permuted single zf-CXXC unit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002894
Physical Location & Seq
Forward (+)
1696560 .. 1707230
10671 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1017600.v1.1.491

Sequence Viewer

Length: 5409 bp
ATGGGTGAACAGGGAGGAGATCAAAGTTCTTGCTGTTGGATGCCACCAACTCCTTACAGGCCTATTCTCACCAGACCGGAGACCAAATTGCGGCCGGAAATTTCGACGTCCTCGAGACCATTGGGGGTAGAAGAAATTCAAGAGAATAGATTAGTTATAGACTTGGATCCGGATCCCGCGGAAGAGAGCGGATTTGTTTACCATGGAGCTGGAGTTGGAGCCGGTAGCGGCGTTGAAACTTCAACGGACGCTTATTTCCGCCGCGTGGCACAGTGGAGAGATGTCCCTTGCAAAGAGCTCATGATCCTCGCGGTGGCCTCTGCGAATGATGGGAGCGGTAGTGCAGTGCTGGGAGATACTCAGCATGAATTTTCATTCCCTGACAACCATCCTTATGATCTGAATATTCCACCTCCGACTACAAATGGCCAATTTGCACCTATAACACCAGATAAGTCCATGAGAGTTGACAGTGAACAAATGTATCGAATTCCGAGCTCCAATGCTGACGATGGACAAGGCCAGGAGATAGAAGAGCAATGGGATGCTAATTCTGCGACAATTAATATCATTGATCTGGAAAACAATAAAGATATAGAAAAATCTGCTGTGGATTCATCACAAGCTACTTTCTCCACGCAGCTCCAGGAACACTGCAACCCTGACAAGGAGGTGAACATCAGTATCGACTTGAACAAGACACCACAACCGAAACAAAGAAGGAGAAAGCACAGGCCAAAGGTGATTATAGAAGGCAAGCCAAAAAGAAACACACAGCCTTCTGTTTCTATGGAAAATCCAAAACCCAAAAGAAAGTATGTGCGCAAGAGTACACTCAATAAAAGCACAACTCCTCCACCGCAGGAATCTACAGAGCATATTGATTCAAATAATCTGCAGCCTACAAAGAGATCATGCAGAAAGGCTTTAAATTTTGACGCAGAAGAACCAAGAGATGGAAGTTCCTCAAGCAAGTCTTTACATGTGGGTTCACAATCACAGGAGATGAATGTTGGCACCAATGGAGTTCAAACAAATTCAACCGCACCACACAGAAATGAAGTTGAATTGGTGGCAGATAACACCCAAGCAGGCATAGCCCAAGATCTCATCCGTTCTACAAGCAGAATGCTGAAACATTACTTGTCATTGCCAGACCAACAACCTCCAAGCACACCACAACAAACGAGAGGTTCTACTACATATGTTGATTCCCAAAAGGAGGCTGCAGAAGAAGTAGGACAAATGAGCACTCATAGTGGATACACTGCACAAACCATGTTGGATCGGGACACTAGATCATCCCAACGAAGTGCAAATGATTCCACCCACAGCACAACTACGGTTCTGACAATAGAAGAACAAGCAAAAAGATCAAAGAGAAATTATTCGAGTGCTGTTGAGCAAGAAGATCCTAGGACCAGAAATTTATATGGGGCAAACTATAATAATTTGCCGGCATACTACAATGTGATGTCATGGGTGCATTTTCCATACATATACAAGAAGAAGAGGACTGATAAGGTGCAAAATTCTACCATACCAAGCACCTCGTATCATGTGAATATGGCAGAAAACATATGGAGACCATCGGCAGCTGGCTGCTTGACTCCTGGTCCTCAAGTAAATGCTGGTAATGTTTCAACTGCACTTGAAGAAGTGGGAAATAACCCTCAGGATAGACCGCAAAGTGTTCACAGTTTCTTGCCCTTGTATCAGACAGAGAGGTCAACAAAGAGAAGATCTAGTTGCCCTACAAGGGTTCGCAACTTGGCTTCACTAACCAGAACACCAGAGCACATACTGCACAGAACCTGTCTCGCCAATCAACCACCCACTGATGGCAATGGTCAAAGAGTGAATCAATTTGACAGATCTCAGACATGCATTGACGCCCTAGTCACAGACGTGGGTGCAACACTCGCAAAAAGGAAGAGGACAAAGAGAAATCCACTTTCCAGTTCACAACGAGGTCTTCTAATATATAAGAACCAGCCATATTTTGCCACAGCATCAGGTGTTCCTCCACAAGTACCATTCGAACAACTGCTTTCTGCTATTACTGAGCACTTCAAATGTTTGGACATTAATAGAGAAAATAGCAGCAGATTTGCCTATCACAGGTATAATGTCAAATCTTCTTACAAGGCACAAGATCAAGAGCACAACGCACTGGTTCTTTATAGAAGAGATGGCACTGTTGTACCATTTGATGGTTCTTTTGATCCAATTAAGAAACGTAAAGCGCGACCTAAAGTTGACCTTGATCAAGAGACTGATAGAGTATGGAAGCTTCTGCTGGAAAATATAAACAGTGAAGGTATTAATGGCACAGATGAGGAGAAAGAAAAATGGTGGGAACAAGAGCGTAAAGTGTTCCGTGGACGTGCAGACAATTTTATTTCACGAATGCATCTTGTGCAAGGAGATAGACGCTTCTCTCCGTGGAAGGGATCAGTTGTGGACTCAGTGGTTGGAGTTTTCCTTACTCAAAATGTTTCAGACCACCTGTCTAGCTCTGCGTTCATGTCAATGGCTGCACATTTTCCCCTGAAGTCAAGGAGCAACGAAATATCATGCGATGAAGAAGTTGCGAGCTTGGTCGTAGATGAACCAGAAGTATGCATTAGTGAGAATTCAAACCAGCCAGGTTGTGACTGGAGTTCCCTAACGTTCCATGACGCTGAGCATAGTGAAGAAAAAGTTGTCAACGGACACAATAACTCTGGAAGCACTACTGAGGGTGTTATCTCAACAAATGAAGCACAATGCAAGCTTTCACATCCATCTGAACCTGGTCCGGGAATGTATCCTAACTCATTAATGAACAGGTCAACCACCAAGATCACAAGAACAGAATTGTACCTGCAGGAGGATATGAGAACGTACAATGGAGTTTCATCTCAAAATTCTGTGGATTCTTCAACTTCACAAACTGTGGAAAAGACAGGATCGTGCGAGAGCAACTCAGAAACAGAGAATCCGCCTAATAGATGTGAAAACAGCAGTTTAGATCACTCCACTTCGTTTGTGGAGCTTTTACAGAGGGCCGAATCAAGTATGCTGCATTACAGTCTCGGGAGCACCCATATGTCCTCTCATGACATATCAAACTGTGGAGGTTACCAACCTGCATGTGTGCAGCATAATGACCAAAGATGTGAGATAAACAGAGAAGCATCCCTTGAGCCTTCCAGTAACTGTTGTTTGAATCTAACCCCCAACCCAGGAGTACAGCAAGTTGAGTACTTTGATTTGTACGGAGAAGTAACCCAGTCTTCTTATGCATCCAAAAATAAATGTGAAGATAGTCCGAGTGAAAGAAGTGCACTAACATCAGAATCTCCCAGTCAAGATACAACACATAACAAGCTGACAGTGAATGTTCAAGAAGCACCAAGATGTTCCGGAAATTCATGCAATAACATTCAGGTAGGTAATAATATGGCCCAGTCTCAACTCGGGTTGGCTGGGAGCTCAAATAATGTTGACATACATTCTCAGGAGCAAAACAGTAAGATACAGCAAAGTTGCTTGAACATTTCTGGAGGGACCACAGATGTTATGCAGAAGGCAACAGAGTTGGGTTCAAATGAGCAGAGTAATTCAGTTAACAAGGAGTTCAGTAGTACGAATGCTGCTACCTCCAACACAAAGAACAGAAAAGCTGGAAAAGAGAAAAAAGATCAGCAAGATTGGGACAAATTAAGGAAACAAGCAGAATTAAATGGAAAGAAAAGGGAAAAGACAGAAAATACAATGGATTCCTTGGACTGGGAAGCCGTAAGATGTGCAGATGTTAATGAGATCGCCCAAACCATCAAAGAGCGGGGGATGAATAATATGCTTGCAGAACGAATCAAGGATTTCCTTAACAGACTGGTCAGAGAGCACGGTAGTGTTGATCTTGAATGGTTGAGGGACGTTCCACCTGACCAAGCAAAAGAGTATCTGCTGAGCTTTCGCGGATTGGGGTTGAAAAGCGTGGAATGTGTGCGGCTTTTGACACTGCACCATCTTGCTTTTCCAGTGGACACAAATGTTGGACGTATAGCTGTACGGCTTGGATGGGTACCCCTTCAGCCCCTGCCTGAGTCATTGCAGTTGCATCTTCTTGAACTGTACCCAGTGCTGGAATCCATACAAAAGTATTTGTGGCCACGACTGTGCAAGCTTGACCAAAGAACATTGTACGAGCTGCATTACCAAATGATAACATTTGGAAAGGTCTTCTGCACGAAAAGCAAGCCGAATTGTAATGCATGCCCGCTGAGAACAGATTGTAGGCATTTTGCAAGCGCATTTGCGAGTGCAAGGCTTGCGCTGCCGGGACCAGAGGAGCAAAGCATAGTGAGTGCAACTGAAGACAGAACTACCCATCCAAACCCTGCTGGTGTCAACAATAGAATGCCCTTGCCCTTCCCTCAGGCAACATACCAGCAATTGGAAGCAAGCCAGAAATCAGAAGTAAAATCTACATTTGGTCATTGTGAACCTACAACGTTCCAGCAATTGGAAGCAAGCCAGATATCTGACGCAAAATCTGCAGTTGGTAAATGTGAACCTATTATTGAAGAACCAGCATCACCAGAGCCAGTGTGTACACAGATATCAGAGGACATTGAGGACTTCTGTGAAGGTCCTGACGAAATTCCAACCATTAAACTTAACATTGAAGAGTTCACTCAGACTTTGCAAAATTATATGGAAAAAAACATGGAGCTTCAAGAAGGTGAAATGTCAAAAGCTTTAGTCTCTCTAACATCAGAAGCTGCATCATTACCAACTCCCAAGCTTAAGAACGTGAGCCGACTGCGAACTGAGCACCAAGTCTATGAACTTCCTGATACGCACCCTCTTCTAGAAATGTTGCATATGGATAAGCGAGAACCTGACGATCCGTGCAATTACCTTCTGGCTATTTGGACTCCAGGTGAAACTCCAAATTCCATTCAACCACCAGAGAAAAGGTGTAGTTCTCAAGAACTTGGCAAATTGTGTGACGATATGGAATGTTTCTCATGCAACAGTGCACGGGAAGCAAATTCACAAACAGTCCGAGGGACTCTCTTGATACCATGCCGAACAGCAATGAGAGGGAGCTTTCCACTTAACGGAACCTACTTTCAAGTTAATGAGGTATTTGCTGACCATGATTCGAGCATTAACCCAATTGATGTTCCAAGGGCTTGGTTGTGGAAGCTATACAGGAGAACAGTGTACTTCGGAACCTCGATACCAACAATATTTAAAGGGTTATCAACACCAGAAATTCAGCACTGCTTCTGGAGAGGATTTGTCTGCGTGAGGGGATTTGACCAAAAGACGCGGGCACCGCGTCCATTAATGGCACGACTGCACTTCCCAGCCAGCAAGTTGGTCAAAACAAAAGATAAGAGAGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003906 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016458 GO:0016787 GO:0016798 GO:0016799 GO:0019104 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031935 GO:0031936 GO:0032259 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035510 GO:0040029 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045815 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0080111 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1803

Amino Acids

201.87

Weight (kDa)

5.99

Isoelectric Point (pI)

55.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Perm-CXXC PF15629 1654 - 1685 4.3e-14 Permuted single zf-CXXC unit
RRM_DME PF15628 1688 - 1788 6.6e-53 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1726, 1898
AatII GACGTC 1 cut(s) 110
Acc16I TGCGCA 1 cut(s) 824
Acc36I ACCTGC 2 cut(s) 2874, 3139
Acc65I GGTACC 1 cut(s) 4072
AccB1I GGYRCC 3 cut(s) 1016, 4072, 5332
AccB7I CCANNNNNTGG 4 cut(s) 956, 2213, 4444, 4513
AccBSI CCGCTC 3 cut(s) 189, 336, 3829
AccII CGCG 7 cut(s) 179, 264, 311, 2248, 3966, 5329, 5338
AccIII TCCGGA 2 cut(s) 169, 3407
AclI AACGTT 2 cut(s) 2672, 4502
AcoI YGGCCR 3 cut(s) 92, 427, 4157
AcuI CTGAAG 3 cut(s) 2573, 4064, 4383
AcyI GRCGYC 2 cut(s) 107, 1893
AflII CTTAAG 1 cut(s) 4796
AflIII ACRYGT 1 cut(s) 982
AhdI GACNNNNNGTC 2 cut(s) 1614, 2509
AjiI CACGTC 2 cut(s) 1909, 2387
AjnI CCWGG 7 cut(s) 522, 645, 1612, 2647, 2794, 3226, 4930
AleI CACNNNNGTG 3 cut(s) 1907, 3897, 4165
AloI GAACNNNNNNTCC 2 cut(s) 4363, 4395
Alw26I GTCTC 8 cut(s) 74, 109, 1579, 1823, 2267, 3080, 3459, 4759
Alw44I GTGCAC 2 cut(s) 3327, 5031
AlwNI CAGNNNCTG 4 cut(s) 1815, 3201, 4087, 4772
Ama87I CYCGRG 3 cut(s) 112, 3077, 3461
Aor13HI TCCGGA 2 cut(s) 169, 3407
AoxI GGCC 9 cut(s) 59, 92, 315, 427, 520, 734, 3048, 3447, 4157
ApaLI GTGCAC 2 cut(s) 3327, 5031
AseI ATTAAT 5 cut(s) 564, 2088, 2325, 2822, 5345
Asp700I GAANNNNTTC 2 cut(s) 135, 1387
Asp718I GGTACC 1 cut(s) 4072
AspA2I CCTAGG 1 cut(s) 1415
AspLEI GCGC 4 cut(s) 825, 2248, 4301, 4324
AspS9I GGNCC 8 cut(s) 1419, 1616, 2798, 3048, 3448, 3552, 4331, 4640
AsuC2I CCSGG 2 cut(s) 2802, 4329
AsuHPI GGTGA 7 cut(s) 17, 61, 685, 754, 4578, 4745, 4946
AsuII TTCGAA 1 cut(s) 2040
AvaI CYCGRG 3 cut(s) 112, 3077, 3461
AvaII GGWCC 6 cut(s) 1419, 1616, 2798, 3552, 4331, 4640
AvrII CCTAGG 1 cut(s) 1415
AxyI CCTNAGG 2 cut(s) 1674, 4425
BaeGI GKGCMC 3 cut(s) 3331, 5035, 5335
BaeI ACNNNNGTAYC 6 cut(s) 467, 500, 667, 700, 2187, 2220
BalI TGGCCA 2 cut(s) 429, 4159
BamHI GGATCC 2 cut(s) 166, 172
BanI GGYRCC 3 cut(s) 1016, 4072, 5332
BanII GRGCYC 3 cut(s) 300, 500, 3479
BarI GAAGNNNNNNTAC 2 cut(s) 2277, 2309
BbsI GAAGAC 4 cut(s) 1967, 3270, 4222, 4371
BceAI ACGGC 2 cut(s) 3767, 4076
BciT130I CCWGG 7 cut(s) 524, 647, 1614, 2649, 2796, 3228, 4932
BciVI GTATCC 2 cut(s) 1256, 2820
BclI TGATCA 1 cut(s) 2266
BcnI CCSGG 2 cut(s) 2802, 4329
BcoDI GTCTC 8 cut(s) 74, 109, 1579, 1823, 2267, 3080, 3459, 4759
BfaI CTAG 6 cut(s) 1296, 1416, 1746, 1898, 2514, 4862
BfmI CTRYAG 5 cut(s) 870, 896, 1227, 2867, 4545
BfrI CTTAAG 1 cut(s) 4796
BfuAI ACCTGC 2 cut(s) 2874, 3139
BfuI GTATCC 2 cut(s) 1256, 2820
BglII AGATCT 3 cut(s) 1105, 1742, 1874
BlnI CCTAGG 1 cut(s) 1415
BlpI GCTNAGC 2 cut(s) 2685, 3956
BmcAI AGTACT 1 cut(s) 3248
Bme1390I CCNGG 9 cut(s) 524, 647, 1614, 2649, 2796, 2802, 3228, 4329, 4932
Bme18I GGWCC 6 cut(s) 1419, 1616, 2798, 3552, 4331, 4640
BmeRI GACNNNNNGTC 2 cut(s) 1614, 2509
BmeT110I CYCGRG 3 cut(s) 112, 3077, 3461
BmgBI CACGTC 2 cut(s) 1909, 2387
BmgT120I GGNCC 8 cut(s) 1419, 1616, 2798, 3048, 3448, 3552, 4331, 4640
BmrFI CCNGG 9 cut(s) 524, 647, 1614, 2649, 2796, 2802, 3228, 4329, 4932
BmrI ACTGGG 5 cut(s) 3268, 3342, 3445, 3784, 4121
BmsI GCATC 9 cut(s) 30, 535, 2021, 2422, 3188, 3296, 4117, 4592, 4784
BmuI ACTGGG 5 cut(s) 3268, 3342, 3445, 3784, 4121
BpiI GAAGAC 4 cut(s) 1967, 3270, 4222, 4371
BplI GAGNNNNNCTC 4 cut(s) 2951, 2983, 5052, 5084
BpmI CTGGAG 6 cut(s) 231, 629, 2680, 3567, 4914, 5308
Bpu1102I GCTNAGC 2 cut(s) 2685, 3956
Bpu14I TTCGAA 1 cut(s) 2040
BpuEI CTTGAG 4 cut(s) 952, 1605, 3206, 4965
BpuMI CCSGG 2 cut(s) 2802, 4329
BsaBI GATNNNNATC 1 cut(s) 171
BsaHI GRCGYC 2 cut(s) 107, 1893
BsaI GGTCTC 3 cut(s) 74, 109, 1579
BsaJI CCNNGG 9 cut(s) 177, 202, 1415, 2380, 2444, 3226, 3768, 5059, 5183
BsaWI WCCGGW 3 cut(s) 76, 169, 3407
BsaXI ACNNNNNCTCC 6 cut(s) 198, 228, 325, 355, 838, 868
Bse118I RCCGGY 2 cut(s) 221, 1456
Bse21I CCTNAGG 2 cut(s) 1674, 4425
Bse3DI GCAATG 5 cut(s) 545, 1148, 1852, 4097, 5097
Bse8I GATNNNNATC 1 cut(s) 171
BseAI TCCGGA 2 cut(s) 169, 3407
BseBI CCWGG 7 cut(s) 524, 647, 1614, 2649, 2796, 3228, 4932
BseDI CCNNGG 9 cut(s) 177, 202, 1415, 2380, 2444, 3226, 3768, 5059, 5183
BseJI GATNNNNATC 1 cut(s) 171
BseMI GCAATG 5 cut(s) 545, 1148, 1852, 4097, 5097
BseRI GAGGAG 4 cut(s) 30, 843, 2354, 4352
BseSI GKGCMC 3 cut(s) 3331, 5035, 5335
BseX3I CGGCCG 1 cut(s) 92
BseYI CCCAGC 3 cut(s) 349, 3470, 5365
Bsh1236I CGCG 7 cut(s) 179, 264, 311, 2248, 3966, 5329, 5338
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 9 cut(s) 61, 94, 317, 429, 522, 736, 3050, 3449, 4159
BshNI GGYRCC 3 cut(s) 1016, 4072, 5332
BsiEI CGRYCG 1 cut(s) 95
BsiHKCI CYCGRG 3 cut(s) 112, 3077, 3461
BsiSI CCGG 8 cut(s) 77, 95, 170, 222, 1457, 2801, 3408, 4328
BslFI GGGAC 7 cut(s) 269, 1304, 3565, 3713, 3935, 4344, 5077
BsmAI GTCTC 8 cut(s) 74, 109, 1579, 1823, 2267, 3080, 3459, 4759
BsmFI GGGAC 7 cut(s) 269, 1304, 3565, 3713, 3935, 4344, 5077
BsmI GAATGC 4 cut(s) 1134, 2415, 3640, 4413
BsnI GGCC 9 cut(s) 61, 94, 317, 429, 522, 736, 3050, 3449, 4159
Bso31I GGTCTC 3 cut(s) 74, 109, 1579
BsoBI CYCGRG 3 cut(s) 112, 3077, 3461
Bsp119I TTCGAA 1 cut(s) 2040
Bsp13I TCCGGA 2 cut(s) 169, 3407
Bsp1407I TGTACA 1 cut(s) 4601
Bsp1720I GCTNAGC 2 cut(s) 2685, 3956
Bsp19I CCATGG 1 cut(s) 202
BspANI GGCC 9 cut(s) 61, 94, 317, 429, 522, 736, 3050, 3449, 4159
BspEI TCCGGA 2 cut(s) 169, 3407
BspFNI CGCG 7 cut(s) 179, 264, 311, 2248, 3966, 5329, 5338
BspHI TCATGA 2 cut(s) 300, 3100
BspMAI CTGCAG 4 cut(s) 900, 1231, 2871, 4549
BspMI ACCTGC 2 cut(s) 2874, 3139
BspQI GCTCTTC 1 cut(s) 528
BspT104I TTCGAA 1 cut(s) 2040
BspT107I GGYRCC 3 cut(s) 1016, 4072, 5332
BspTI CTTAAG 1 cut(s) 4796
BspTNI GGTCTC 3 cut(s) 74, 109, 1579
BsrBI CCGCTC 3 cut(s) 189, 336, 3829
BsrDI GCAATG 5 cut(s) 545, 1148, 1852, 4097, 5097
BsrFI RCCGGY 2 cut(s) 221, 1456
BsrGI TGTACA 1 cut(s) 4601
BssAI RCCGGY 2 cut(s) 221, 1456
BssECI CCNNGG 9 cut(s) 177, 202, 1415, 2380, 2444, 3226, 3768, 5059, 5183
BssNI GRCGYC 2 cut(s) 107, 1893
BssT1I CCWWGG 4 cut(s) 202, 1415, 3768, 5183
Bst2UI CCWGG 7 cut(s) 524, 647, 1614, 2649, 2796, 3228, 4932
Bst6I CTCTTC 7 cut(s) 177, 528, 1505, 1928, 2182, 4671, 4863
BstACI GRCGYC 2 cut(s) 107, 1893
BstAFI CTTAAG 1 cut(s) 4796
BstAPI GCANNNNNTGC 4 cut(s) 1804, 2419, 4319, 4544
BstAUI TGTACA 1 cut(s) 4601
BstBI TTCGAA 1 cut(s) 2040
BstDSI CCRYGG 4 cut(s) 177, 202, 2380, 2444
BstEII GGTNACC 1 cut(s) 3122
BstENI CCTNNNNNAGG 2 cut(s) 2119, 2870
BstFNI CGCG 7 cut(s) 179, 264, 311, 2248, 3966, 5329, 5338
BstHHI GCGC 4 cut(s) 825, 2248, 4301, 4324
BstMAI GTCTC 8 cut(s) 74, 109, 1579, 1823, 2267, 3080, 3459, 4759
BstMCI CGRYCG 1 cut(s) 95
BstNI CCWGG 7 cut(s) 524, 647, 1614, 2649, 2796, 3228, 4932
BstNSI RCATGY 4 cut(s) 986, 1887, 3138, 4266
BstPI GGTNACC 1 cut(s) 3122
BstSCI CCNGG 9 cut(s) 522, 645, 1612, 2647, 2794, 2800, 3226, 4327, 4930
BstSFI CTRYAG 5 cut(s) 870, 896, 1227, 2867, 4545
BstSLI GKGCMC 3 cut(s) 3331, 5035, 5335
BstUI CGCG 7 cut(s) 179, 264, 311, 2248, 3966, 5329, 5338
BstV2I GAAGAC 4 cut(s) 1967, 3270, 4222, 4371
BstX2I RGATCY 6 cut(s) 166, 172, 1105, 1411, 1742, 1874
BstXI CCANNNNNNTGG 3 cut(s) 209, 5190, 5377
BstYI RGATCY 6 cut(s) 166, 172, 1105, 1411, 1742, 1874
BstZI CGGCCG 1 cut(s) 92
Bsu36I CCTNAGG 2 cut(s) 1674, 4425
BsuI GTATCC 2 cut(s) 1256, 2820
BsuRI GGCC 9 cut(s) 61, 94, 317, 429, 522, 736, 3050, 3449, 4159
BtgI CCRYGG 4 cut(s) 177, 202, 2380, 2444
BtgZI GCGATG 1 cut(s) 2595
BtrI CACGTC 2 cut(s) 1909, 2387
BtsI GCAGTG 5 cut(s) 351, 652, 1266, 4007, 5278
BveI ACCTGC 2 cut(s) 2874, 3139
CaiI CAGNNNCTG 4 cut(s) 1815, 3201, 4087, 4772
CciI TCATGA 2 cut(s) 300, 3100
CfoI GCGC 4 cut(s) 825, 2248, 4301, 4324
Cfr10I RCCGGY 2 cut(s) 221, 1456
Cfr13I GGNCC 8 cut(s) 1419, 1616, 2798, 3048, 3448, 3552, 4331, 4640
Cfr42I CCGCGG 1 cut(s) 180
CseI GACGC 8 cut(s) 257, 947, 1901, 2442, 2690, 4544, 5327, 5335
CsiI ACCWGGT 1 cut(s) 2794
CspCI CAANNNNNGTGG 2 cut(s) 3010, 3045
DraI TTTAAA 2 cut(s) 930, 5251
DrdI GACNNNNNNGTC 2 cut(s) 1726, 1898
DriI GACNNNNNGTC 2 cut(s) 1614, 2509
DseDI GACNNNNNNGTC 2 cut(s) 1726, 1898
EaeI YGGCCR 3 cut(s) 92, 427, 4157
EagI CGGCCG 1 cut(s) 92
Eam1104I CTCTTC 7 cut(s) 177, 528, 1505, 1928, 2182, 4671, 4863
Eam1105I GACNNNNNGTC 2 cut(s) 1614, 2509
EarI CTCTTC 7 cut(s) 177, 528, 1505, 1928, 2182, 4671, 4863
EciI GGCGGA 2 cut(s) 248, 2973
Ecl136II GAGCTC 3 cut(s) 298, 498, 3477
EclXI CGGCCG 1 cut(s) 92
Eco130I CCWWGG 4 cut(s) 202, 1415, 3768, 5183
Eco147I AGGCCT 1 cut(s) 61
Eco24I GRGCYC 3 cut(s) 300, 500, 3479
Eco31I GGTCTC 3 cut(s) 74, 109, 1579
Eco32I GATATC 2 cut(s) 4530, 4611
Eco47I GGWCC 6 cut(s) 1419, 1616, 2798, 3552, 4331, 4640
Eco52I CGGCCG 1 cut(s) 92
Eco53kI GAGCTC 3 cut(s) 298, 498, 3477
Eco57I CTGAAG 3 cut(s) 2573, 4064, 4383
Eco81I CCTNAGG 2 cut(s) 1674, 4425
Eco88I CYCGRG 3 cut(s) 112, 3077, 3461
Eco91I GGTNACC 1 cut(s) 3122
EcoICRI GAGCTC 3 cut(s) 298, 498, 3477
EcoNI CCTNNNNNAGG 2 cut(s) 2119, 2870
EcoO109I RGGNCCY 1 cut(s) 4640
EcoO65I GGTNACC 1 cut(s) 3122
EcoRI GAATTC 2 cut(s) 489, 2635
EcoRII CCWGG 7 cut(s) 522, 645, 1612, 2647, 2794, 3226, 4930
EcoRV GATATC 2 cut(s) 4530, 4611
EcoT14I CCWWGG 4 cut(s) 202, 1415, 3768, 5183
EcoT22I ATGCAT 5 cut(s) 1889, 2415, 2627, 3289, 4264
EcoT38I GRGCYC 3 cut(s) 300, 500, 3479
ErhI CCWWGG 4 cut(s) 202, 1415, 3768, 5183
FalI AAGNNNNNCTT 6 cut(s) 961, 993, 2247, 2279, 3168, 3200
FaqI GGGAC 7 cut(s) 269, 1304, 3565, 3713, 3935, 4344, 5077
FauI CCCGC 4 cut(s) 184, 3822, 4275, 5322
FauNDI CATATG 4 cut(s) 1204, 1580, 3090, 4875
FbaI TGATCA 1 cut(s) 2266
FriOI GRGCYC 3 cut(s) 300, 500, 3479
FspBI CTAG 6 cut(s) 1296, 1416, 1746, 1898, 2514, 4862
FspI TGCGCA 1 cut(s) 824
GlaI GCGC 4 cut(s) 824, 2247, 4300, 4323
GsaI CCCAGC 3 cut(s) 353, 3474, 5369
GsuI CTGGAG 6 cut(s) 231, 629, 2680, 3567, 4914, 5308
HaeIII GGCC 9 cut(s) 61, 94, 317, 429, 522, 736, 3050, 3449, 4159
HapII CCGG 8 cut(s) 77, 95, 170, 222, 1457, 2801, 3408, 4328
HgaI GACGC 8 cut(s) 257, 947, 1901, 2442, 2690, 4544, 5327, 5335
HhaI GCGC 4 cut(s) 825, 2248, 4301, 4324
Hin1I GRCGYC 2 cut(s) 107, 1893
Hin6I GCGC 4 cut(s) 823, 2246, 4299, 4322
HinP1I GCGC 4 cut(s) 823, 2246, 4299, 4322
HincII GTYRAC 8 cut(s) 469, 1731, 2260, 2710, 2835, 3490, 3613, 4399
HindII GTYRAC 8 cut(s) 469, 1731, 2260, 2710, 2835, 3490, 3613, 4399
HindIII AAGCTT 5 cut(s) 2291, 2774, 4172, 4746, 4793
HpaI GTTAAC 1 cut(s) 3613
HpaII CCGG 8 cut(s) 77, 95, 170, 222, 1457, 2801, 3408, 4328
HphI GGTGA 7 cut(s) 17, 61, 685, 754, 4578, 4745, 4946
Hpy99I CGWCG 1 cut(s) 109
Hsp92I GRCGYC 2 cut(s) 107, 1893
HspAI GCGC 4 cut(s) 823, 2246, 4299, 4322
Kpn2I TCCGGA 2 cut(s) 169, 3407
KpnI GGTACC 1 cut(s) 4076
KroI GCCGGC 1 cut(s) 1456
KroNI GCCGGC 1 cut(s) 1458
Ksp22I TGATCA 1 cut(s) 2266
KspAI GTTAAC 1 cut(s) 3613
KspI CCGCGG 1 cut(s) 180
LguI GCTCTTC 1 cut(s) 528
LweI GCATC 9 cut(s) 30, 535, 2021, 2422, 3188, 3296, 4117, 4592, 4784
MabI ACCWGGT 1 cut(s) 2794
MaeI CTAG 6 cut(s) 1296, 1416, 1746, 1898, 2514, 4862
MaeIII GTNAC 6 cut(s) 1900, 2654, 3122, 3197, 3268, 5000
MbiI CCGCTC 3 cut(s) 189, 336, 3829
MfeI CAATTG 3 cut(s) 4442, 4511, 5172
MflI RGATCY 6 cut(s) 166, 172, 1105, 1411, 1742, 1874
MlsI TGGCCA 2 cut(s) 429, 4159
MluNI TGGCCA 2 cut(s) 429, 4159
MlyI GAGTC 5 cut(s) 1603, 2459, 4103, 4921, 5059
MmeI TCCRAC 8 cut(s) 17, 196, 440, 1263, 2455, 3672, 4024, 4679
Mox20I TGGCCA 2 cut(s) 429, 4159
Mph1103I ATGCAT 5 cut(s) 1889, 2415, 2627, 3289, 4264
MroI TCCGGA 2 cut(s) 169, 3407
MroNI GCCGGC 1 cut(s) 1456
MroXI GAANNNNTTC 2 cut(s) 135, 1387
MscI TGGCCA 2 cut(s) 429, 4159
MslI CAYNNNNRTG 8 cut(s) 393, 1056, 1907, 2531, 3089, 3400, 3897, 4165
Msp20I TGGCCA 2 cut(s) 429, 4159
MspA1I CMGCKG 3 cut(s) 179, 1598, 4270
MspCI CTTAAG 1 cut(s) 4796
MspI CCGG 8 cut(s) 77, 95, 170, 222, 1457, 2801, 3408, 4328
MspR9I CCNGG 9 cut(s) 524, 647, 1614, 2649, 2796, 2802, 3228, 4329, 4932
MunI CAATTG 3 cut(s) 4442, 4511, 5172
Mva1269I GAATGC 4 cut(s) 1134, 2415, 3640, 4413
MvaI CCWGG 7 cut(s) 524, 647, 1614, 2649, 2796, 3228, 4932
MvnI CGCG 7 cut(s) 179, 264, 311, 2248, 3966, 5329, 5338
NaeI GCCGGC 1 cut(s) 1458
NciI CCSGG 2 cut(s) 2802, 4329
NcoI CCATGG 1 cut(s) 202
NdeI CATATG 4 cut(s) 1204, 1580, 3090, 4875
NgoMIV GCCGGC 1 cut(s) 1456
NmuCI GTSAC 3 cut(s) 1900, 2654, 5000
NsbI TGCGCA 1 cut(s) 824
NsiI ATGCAT 5 cut(s) 1889, 2415, 2627, 3289, 4264
NspI RCATGY 4 cut(s) 986, 1887, 3138, 4266
NspV TTCGAA 1 cut(s) 2040
OliI CACNNNNGTG 3 cut(s) 1907, 3897, 4165
PaeI GCATGC 1 cut(s) 4266
PaeR7I CTCGAG 1 cut(s) 112
PagI TCATGA 2 cut(s) 300, 3100
PceI AGGCCT 1 cut(s) 61
PciI ACATGT 1 cut(s) 982
PciSI GCTCTTC 1 cut(s) 528
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 2245
PctI GAATGC 4 cut(s) 1134, 2415, 3640, 4413
PdiI GCCGGC 1 cut(s) 1458
PdmI GAANNNNTTC 2 cut(s) 135, 1387
PflMI CCANNNNNTGG 4 cut(s) 956, 2213, 4444, 4513
PfoI TCCNGGA 2 cut(s) 645, 2800
PleI GAGTC 5 cut(s) 1603, 2459, 4102, 4921, 5059
PpsI GAGTC 5 cut(s) 1603, 2459, 4102, 4921, 5059
PpuMI RGGWCCY 1 cut(s) 4640
PscI ACATGT 1 cut(s) 982
PshBI ATTAAT 5 cut(s) 564, 2088, 2325, 2822, 5345
Psp124BI GAGCTC 3 cut(s) 300, 500, 3479
Psp1406I AACGTT 2 cut(s) 2672, 4502
Psp5II RGGWCCY 1 cut(s) 4640
Psp6I CCWGG 7 cut(s) 522, 645, 1612, 2647, 2794, 3226, 4930
PspEI GGTNACC 1 cut(s) 3122
PspFI CCCAGC 3 cut(s) 349, 3470, 5365
PspGI CCWGG 7 cut(s) 522, 645, 1612, 2647, 2794, 3226, 4930
PspPI GGNCC 8 cut(s) 1419, 1616, 2798, 3048, 3448, 3552, 4331, 4640
PspPPI RGGWCCY 1 cut(s) 4640
PstI CTGCAG 4 cut(s) 900, 1231, 2871, 4549
PstNI CAGNNNCTG 4 cut(s) 1815, 3201, 4087, 4772
PsuI RGATCY 6 cut(s) 166, 172, 1105, 1411, 1742, 1874
PvuII CAGCTG 1 cut(s) 1598
RseI CAYNNNNRTG 8 cut(s) 393, 1056, 1907, 2531, 3089, 3400, 3897, 4165
SacI GAGCTC 3 cut(s) 300, 500, 3479
SacII CCGCGG 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 528
Sau96I GGNCC 8 cut(s) 1419, 1616, 2798, 3048, 3448, 3552, 4331, 4640
SbfI CCTGCAGG 1 cut(s) 2871
ScaI AGTACT 1 cut(s) 3248
SchI GAGTC 5 cut(s) 1603, 2459, 4103, 4921, 5059
ScrFI CCNGG 9 cut(s) 524, 647, 1614, 2649, 2796, 2802, 3228, 4329, 4932
SdaI CCTGCAGG 1 cut(s) 2871
SexAI ACCWGGT 1 cut(s) 2794
SfaNI GCATC 9 cut(s) 30, 535, 2021, 2422, 3188, 3296, 4117, 4592, 4784
SfcI CTRYAG 5 cut(s) 870, 896, 1227, 2867, 4545
Sfr274I CTCGAG 1 cut(s) 112
Sfr303I CCGCGG 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 2040
SgrBI CCGCGG 1 cut(s) 180
SinI GGWCC 6 cut(s) 1419, 1616, 2798, 3552, 4331, 4640
SlaI CTCGAG 1 cut(s) 112
SmiMI CAYNNNNRTG 8 cut(s) 393, 1056, 1907, 2531, 3089, 3400, 3897, 4165
SmlI CTYRAG 6 cut(s) 112, 967, 1620, 3185, 4796, 4980
SmoI CTYRAG 6 cut(s) 112, 967, 1620, 3185, 4796, 4980
SphI GCATGC 1 cut(s) 4266
Sse8387I CCTGCAGG 1 cut(s) 2871
SseBI AGGCCT 1 cut(s) 61
SspI AATATT 2 cut(s) 406, 5247
SspMI CTAG 6 cut(s) 1296, 1416, 1746, 1898, 2514, 4862
SstI GAGCTC 3 cut(s) 300, 500, 3479
StuI AGGCCT 1 cut(s) 61
StyD4I CCNGG 9 cut(s) 522, 645, 1612, 2647, 2794, 2800, 3226, 4327, 4930
StyI CCWWGG 4 cut(s) 202, 1415, 3768, 5183
TaqI TCGA 8 cut(s) 104, 113, 487, 687, 1391, 2040, 5159, 5234
TatI WGTACW 5 cut(s) 830, 3232, 3246, 4601, 5220
TauI GCSGC 4 cut(s) 94, 231, 264, 4000
TseFI GTSAC 3 cut(s) 1900, 2654, 5000
Tsp45I GTSAC 3 cut(s) 1900, 2654, 5000
TspGWI ACGGA 8 cut(s) 260, 1103, 2369, 2433, 2727, 3276, 4890, 5130
Van91I CCANNNNNTGG 4 cut(s) 956, 2213, 4444, 4513
Vha464I CTTAAG 1 cut(s) 4796
VneI GTGCAC 2 cut(s) 3327, 5031
VpaK11BI GGWCC 6 cut(s) 1419, 1616, 2798, 3552, 4331, 4640
VspI ATTAAT 5 cut(s) 564, 2088, 2325, 2822, 5345
XagI CCTNNNNNAGG 2 cut(s) 2119, 2870
XbaI TCTAGA 1 cut(s) 4861
XceI RCATGY 4 cut(s) 986, 1887, 3138, 4266
XcmI CCANNNNNNNNNTGG 2 cut(s) 2655, 3028
XhoI CTCGAG 1 cut(s) 112
XmaJI CCTAGG 1 cut(s) 1415
XmnI GAANNNNTTC 2 cut(s) 135, 1387
XspI CTAG 6 cut(s) 1296, 1416, 1746, 1898, 2514, 4862
ZraI GACGTC 1 cut(s) 108
ZrmI AGTACT 1 cut(s) 3248
Zsp2I ATGCAT 5 cut(s) 1889, 2415, 2627, 3289, 4264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.