pycom12g01430

Permuted single zf-CXXC unit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002894
Physical Location & Seq
Forward (+)
1196790 .. 1197715
926 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g01430.3

Sequence Viewer

Length: 582 bp
ATGCATCTTGTGCAAGGAGATAGACGCTTCTCTCCGTGGAAGGGATCAGTTGTGGACTCAGTGGTTGGAGTTTTCCTTACTCAAAATGTCTCAGACCACCTGTCTAGCTCTGCGTTCATGTCAATGGCTGCACATTTTCCCCTGAAGTCAAGGAGCAACGAAATATCATGCGATGAAGAAGTTGCGAGTTTGGTAGTAGATGAACCAGAAGTATGCATTAGTGAGAATTCAAACCAGCCAGGTTGTGACTGGAGTTCCCTAACGTTCCATGACGCTGAGCATAGTGAAGAAAAAGTTGTCAACGGACACGATAACTCTGGAAGCACTACTGAGGGTGTTATCTCAACAAATGAAGCACAATGCAAGCTTTCACATCCATCTGAACCTGGTCCGGGAATGTATCCTAACTCATTAATGAACAGGTCAACCACCAAGATCACAAGAACAGAATTGTACCTGCGGGAGGATATGAGAACGTACAATGGAGTTTCATCTCAAAATTCTGAGGATTCTTCAACTTCACAAACTGTGGAAAAGACAGGATCGATCCGAGAGCAACTCGGAAACAGAGAATCCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003906 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016458 GO:0016787 GO:0016798 GO:0016799 GO:0019104 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031935 GO:0031936 GO:0032259 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035510 GO:0040029 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045815 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0080111 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

194

Amino Acids

21.18

Weight (kDa)

4.85

Isoelectric Point (pI)

52.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 465
AciI CCGC 1 cut(s) 460
AclI AACGTT 1 cut(s) 263
AclWI GGATC 3 cut(s) 52, 541, 550
AcsI RAATTY 2 cut(s) 226, 499
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 2 cut(s) 455, 479
AfiI CCNNNNNNNGG 3 cut(s) 41, 392, 463
AgsI TTSAA 2 cut(s) 231, 516
AhdI GACNNNNNGTC 1 cut(s) 100
AjnI CCWGG 2 cut(s) 238, 385
AluBI AGCT 2 cut(s) 108, 367
AluI AGCT 2 cut(s) 108, 367
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 3 cut(s) 52, 541, 550
ApeKI GCWGC 1 cut(s) 128
ApoI RAATTY 2 cut(s) 226, 499
AseI ATTAAT 1 cut(s) 413
AspS9I GGNCC 1 cut(s) 389
AsuC2I CCSGG 1 cut(s) 393
AvaII GGWCC 1 cut(s) 389
BbvI GCAGC 1 cut(s) 115
BccI CCATC 1 cut(s) 385
BciT130I CCWGG 2 cut(s) 240, 387
BciVI GTATCC 1 cut(s) 411
BcnI CCSGG 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 465
BfuI GTATCC 1 cut(s) 411
BisI GCNGC 1 cut(s) 129
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 130
Bme1390I CCNGG 3 cut(s) 240, 387, 393
Bme18I GGWCC 1 cut(s) 389
BmeRI GACNNNNNGTC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 389
BmrFI CCNGG 3 cut(s) 240, 387, 393
BmsI GCATC 1 cut(s) 13
BplI GAGNNNNNCTC 2 cut(s) 543, 575
BpmI CTGGAG 1 cut(s) 271
Bpu1102I GCTNAGC 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 393
Bsa29I ATCGAT 1 cut(s) 545
BsaJI CCNNGG 1 cut(s) 35
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 392, 463
Bse1I ACTGG 1 cut(s) 254
BseBI CCWGG 2 cut(s) 240, 387
BseCI ATCGAT 1 cut(s) 545
BseDI CCNNGG 1 cut(s) 35
BseGI GGATG 1 cut(s) 373
BseLI CCNNNNNNNGG 3 cut(s) 41, 392, 463
BseMII CTCAG 5 cut(s) 72, 105, 267, 321, 495
BseNI ACTGG 1 cut(s) 254
BseXI GCAGC 1 cut(s) 115
BsgI GTGCAG 1 cut(s) 114
BshVI ATCGAT 1 cut(s) 545
BsiSI CCGG 1 cut(s) 392
BslI CCNNNNNNNGG 3 cut(s) 41, 392, 463
BsmAI GTCTC 1 cut(s) 94
Bsp143I GATC 4 cut(s) 44, 435, 542, 546
Bsp1720I GCTNAGC 1 cut(s) 276
BspACI CCGC 1 cut(s) 460
BspCNI CTCAG 5 cut(s) 71, 104, 268, 322, 496
BspDI ATCGAT 1 cut(s) 545
BspMI ACCTGC 1 cut(s) 465
BspPI GGATC 3 cut(s) 52, 541, 550
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 4 cut(s) 44, 435, 542, 546
Bst2UI CCWGG 2 cut(s) 240, 387
Bst4CI ACNGT 1 cut(s) 529
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 365
BstDEI CTNAG 5 cut(s) 58, 91, 276, 330, 504
BstDSI CCRYGG 1 cut(s) 35
BstENI CCTNNNNNAGG 1 cut(s) 461
BstF5I GGATG 1 cut(s) 373
BstKTI GATC 4 cut(s) 47, 438, 545, 549
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 4 cut(s) 44, 435, 542, 546
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 2 cut(s) 240, 387
BstSCI CCNGG 3 cut(s) 238, 385, 391
BstV1I GCAGC 1 cut(s) 115
Bsu15I ATCGAT 1 cut(s) 545
BsuI GTATCC 1 cut(s) 411
BsuTUI ATCGAT 1 cut(s) 545
BtgI CCRYGG 1 cut(s) 35
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 373
BtsIMutI CAGTG 1 cut(s) 66
BveI ACCTGC 1 cut(s) 465
Cac8I GCNNGC 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 389
ClaI ATCGAT 1 cut(s) 545
CseI GACGC 2 cut(s) 33, 281
CsiI ACCWGGT 1 cut(s) 385
Csp6I GTAC 2 cut(s) 454, 478
CviAII CATG 3 cut(s) 118, 168, 269
CviJI RGCY 4 cut(s) 108, 128, 238, 367
CviKI_1 RGCY 4 cut(s) 108, 128, 238, 367
CviQI GTAC 2 cut(s) 454, 478
DdeI CTNAG 5 cut(s) 58, 91, 276, 330, 504
DpnI GATC 4 cut(s) 46, 437, 544, 548
DpnII GATC 4 cut(s) 44, 435, 542, 546
DriI GACNNNNNGTC 1 cut(s) 100
Eam1105I GACNNNNNGTC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 389
Eco57I CTGAAG 1 cut(s) 164
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoRI GAATTC 1 cut(s) 226
EcoRII CCWGG 2 cut(s) 238, 385
EcoT22I ATGCAT 2 cut(s) 6, 218
FaeI CATG 3 cut(s) 121, 171, 272
FaiI YATR 6 cut(s) 119, 169, 214, 270, 282, 470
FatI CATG 3 cut(s) 117, 167, 268
FauI CCCGC 1 cut(s) 453
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 1 cut(s) 360
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 1 cut(s) 105
GluI GCNGC 1 cut(s) 129
GsuI CTGGAG 1 cut(s) 271
HapII CCGG 1 cut(s) 392
HgaI GACGC 2 cut(s) 33, 281
Hin1II CATG 3 cut(s) 121, 171, 272
HincII GTYRAC 2 cut(s) 301, 426
HindII GTYRAC 2 cut(s) 301, 426
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 3 cut(s) 56, 509, 572
HpaII CCGG 1 cut(s) 392
Hpy166II GTNNAC 3 cut(s) 55, 301, 426
Hpy188I TCNGA 5 cut(s) 94, 382, 505, 551, 563
Hpy188III TCNNGA 1 cut(s) 318
Hpy8I GTNNAC 3 cut(s) 55, 301, 426
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4IV ACGT 2 cut(s) 263, 476
HpyCH4V TGCA 5 cut(s) 4, 13, 131, 216, 363
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 5 cut(s) 58, 91, 276, 330, 504
HpySE526I ACGT 2 cut(s) 263, 476
Hsp92II CATG 3 cut(s) 121, 171, 272
Kzo9I GATC 4 cut(s) 44, 435, 542, 546
LmnI GCTCC 1 cut(s) 153
Lsp1109I GCAGC 1 cut(s) 115
LweI GCATC 1 cut(s) 13
MabI ACCWGGT 1 cut(s) 385
MaeI CTAG 1 cut(s) 105
MaeII ACGT 2 cut(s) 263, 476
MaeIII GTNAC 1 cut(s) 245
MalI GATC 4 cut(s) 46, 437, 544, 548
MboI GATC 4 cut(s) 44, 435, 542, 546
MboII GAAGA 3 cut(s) 188, 299, 504
MluCI AATT 3 cut(s) 226, 449, 499
MlyI GAGTC 1 cut(s) 50
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 3 cut(s) 325, 457, 499
Mph1103I ATGCAT 2 cut(s) 6, 218
MseI TTAA 1 cut(s) 413
MslI CAYNNNNRTG 1 cut(s) 122
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 3 cut(s) 240, 387, 393
MvaI CCWGG 2 cut(s) 240, 387
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 1 cut(s) 393
NdeII GATC 4 cut(s) 44, 435, 542, 546
NlaIII CATG 3 cut(s) 121, 171, 272
NmuCI GTSAC 1 cut(s) 245
NsiI ATGCAT 2 cut(s) 6, 218
PfeI GAWTC 2 cut(s) 509, 572
PfoI TCCNGGA 1 cut(s) 391
PkrI GCNGC 1 cut(s) 130
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 413
Psp1406I AACGTT 1 cut(s) 263
Psp6I CCWGG 2 cut(s) 238, 385
PspGI CCWGG 2 cut(s) 238, 385
PspPI GGNCC 1 cut(s) 389
RsaI GTAC 2 cut(s) 455, 479
RsaNI GTAC 2 cut(s) 454, 478
RseI CAYNNNNRTG 1 cut(s) 122
SaqAI TTAA 1 cut(s) 413
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 4 cut(s) 44, 435, 542, 546
Sau96I GGNCC 1 cut(s) 389
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 3 cut(s) 240, 387, 393
SexAI ACCWGGT 1 cut(s) 385
SfaNI GCATC 1 cut(s) 13
SinI GGWCC 1 cut(s) 389
SmiMI CAYNNNNRTG 1 cut(s) 122
Sse9I AATT 3 cut(s) 226, 449, 499
SsiI CCGC 1 cut(s) 460
SspMI CTAG 1 cut(s) 105
StyD4I CCNGG 3 cut(s) 238, 385, 391
TaaI ACNGT 1 cut(s) 529
TaiI ACGT 2 cut(s) 266, 479
TaqI TCGA 1 cut(s) 545
TasI AATT 3 cut(s) 226, 449, 499
TfiI GAWTC 2 cut(s) 509, 572
Tru1I TTAA 1 cut(s) 413
Tru9I TTAA 1 cut(s) 413
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 6 cut(s) 106, 189, 216, 366, 431, 480
TspGWI ACGGA 2 cut(s) 24, 318
TspRI CASTG 1 cut(s) 66
VpaK11BI GGWCC 1 cut(s) 389
VspI ATTAAT 1 cut(s) 413
XagI CCTNNNNNAGG 1 cut(s) 461
XapI RAATTY 2 cut(s) 226, 499
XcmI CCANNNNNNNNNTGG 1 cut(s) 246
XspI CTAG 1 cut(s) 105
Zsp2I ATGCAT 2 cut(s) 6, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.