Rmu_sc0002532.1_g000017

Permuted single zf-CXXC unit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002532.1
OG0002894
Physical Location & Seq
Forward (+)
92903 .. 101761
8859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002532.1_g000017.1.cds

Sequence Viewer

Length: 5166 bp
atggatatgaatgatcagagaaaagatgccttccaagactggccttcttgcattgctccaacaactccttacaggcctattctcccaaaaccacctcaagaacataaacaccatgaattttttatgcccccaaataaattcccgtttgctttaccagccaatgaagggaataatggagctggacctgttggtgccggcaacacactcgagatgtcgagagaggttccttgtatagatctcctggcccttgcccatgccgcctctagcgcagctgataataatgctgtcatgcatggagaacctcagtatgatgttccctttcctcaccaccttccttatgatctaaattcactacccgagaccacctatggccaatttgcacccataacaccagagaaggcaagctcaaatgcggatcataaaaaagaccagcaaattgaagagcagatgaatgctggtgctaccacatttgaaatctttgagctgggaaataagaaagatgtagcagaccctgctacagattcttcacatgctactttttccacacaagtccaggagaatcagacacagctccaggataatcacatcatcaaggaagggaatgatagcattgacttgaataagacaccgcagctgaaacaaaggaggagaaagcacaggcccaaggttattagagaaggcaagccaaaaccactgccgaaacctcctactactaaggaaactccagccagaagaaagtatgtaaggaagaatgcactagacaaaaatgcaactcctcccccaccaaaggaacttggggagtgtaccgattcaaataatctgaagtccaccaagagatcatgcagaagggtgttaaattatgacatggaagaaccaggagatgatatatcctcctgcagatctttcaattcaggtttggactcacagatgcgtaatttttgcaccaatggagttgcatcaaaatcaactgtgcagctcagaaatggaattaattcaatggtagataacactcggatggagttagcccatgaacttgttctttctacaaatcaatggctgaaagaacacttgtcactgcctgaacaacaatctccagccacaccgtgtcctgccagaaatagttccgtgcagccaagagagtatgttgattgccaacaagacactgcagaaggaaaagcaagagtgagtgctcaactctgtcacaaaaatgctgtacaaaccatgttggatggtgacactagatcctcttcaccaaggccaaatgactccaactgcagctcatccatgattctgacacaagaaaatgaactgaagggatccaagcgaaaatattataatgctgttcagcagacagatccaagtacccgaaatttacttggggttcactacaataatttgccagcatatgacaacatgatgtcttatatgcattttccatacatatacaagaagaagaggactgataaggcatatacttctatcatatcaagcacatcatgtcgtgtgactatggcagaaaatgtttggagacaatcaaaactgcaagctgttgagtctatcctgccttcttaccagacgcagagttcaaaaaagagaagatcaaaagctcctacacgggttcgtgacttggcttctctaattagaacaccagagcacattctgcgccagagtacctgtctcaccaaaccaccagctgatggtaatgggcaaagacgtatgaactgtaactcatctcagacatgcatggatgccctagtcactgacgtgggtgccacactcgcaaagaaaaagagaacaaagagaagttctgttaccagttcacaccgaagtattgtgctatacaagaaccaaccatttgttcccggatcatcaggtgttccaccagaagtagcatgcacacaaattctttccgttgatgctattactgatcaattaaaatgtttgaacttaaatagagagaacagcaaatttgcatatcaggggtataatgttgtctataatacacaaaagcaagagaacaatgctttggttctttatagaagagatggcactgttgtaccatttgagggggcttttgatcctatcaagaaacgtcggccacgacctaaagttgaccttgatgaagagacggataaagtatggaagctgctgatggataacataaacagtgaaggcgttgatggaacagatgaacagaaagcaaaatggtgggaagaagagcggagagtgttccaaggtcgcgcagactcttttatcgcacgaatgcatcttgtgcaaggagatagacgcttctctccatggaaaggatcagttgtggactcagtggtgggagttttcctgactcaaaatgtgtcagatcatctgtctagctctgcattcatgtcgctcgcagcacgttttcccctgaagtcagtgagcaaccaaaatgcatctgatgaaaaagttgcaggcttagcagtagatgaaccagaagtgtgcattagtgagatttcaaaccagccactctgtgattctagttccgtaacattccatgacactgagcatagtgaagaaaaagttgtcaacagcaatgagaacacagaaaccactagtgagggagttatctctacaaatgaacctgactgcaagctttcacattcattggccaacaggtccaccaccataaacccaagaacagcatcagaatgttgcatagaggaagacttgagaacaggatatgatatagtttcatctcaaaattctgtggattcttcaacttctcacactgtggaaaaagcaggatcatgtgagagcaactcagagacagaggacacgcctaatacatgtcaaaacggcagtttggaccattccacttcgtttttacagaaggccgcagtctacagtctcaaaaccagccatatgtcttctcatgacaatttgaattgtgaactttatgaacccatatgcatggagcatgatgaccaaagaagatatatagacaggggaggagcatcccaggacccttccaataatttttgtttgcatattacctccaacccagaagtagtgcaagttgagtgctctgatttgttcgaagaggtaatccaatcttctaatatttccaagaacaaatatgaagacagtctgggggaacagagtgtgctaacagcagaatctgcgagtcaagatacaataagtaataagcttactgtgaatgatcaagatgcacagagatgtttcaatgaatcatgcaattgcattgagggaaaaaataacttggttcagtctcaattcagggtgggtgggaacccgaataaggttgatgtacctgctgaggagcataaaaataagattcagcaaaattgcaacatttctcgtgagaccacagatattatgcataagggaccagagtcagatttaagcttcaacgaggtcagtaaggtggatgctgctacctcaaagacaaagaacagaagacctggaaaagagaaaaagcagcaggactgggacaaattaagagaacgagcagaaccaaatggaagaaaaagggagaaaacagcaaatacaatggattcagtggactgggaagctgtaagaactgcaaatgtaaatgacattgcccaaactatcaaagagagggggatgaataatatgctcgcggaaagaatcaaggaattccttaaccgactggttagagaacacgggagcgttgatcttgaatggttaagagatgttccacctgaccaagcaaaagagtatctgctgagcttccgaggactgggtctaaaaagcgtggagtgtgtgaggcttttgacacttcatcatcttgcttttccggtcgatacaaatgttggacgtattgctgtacggctgggatgggtaccccttcagcccttgcccgagtcactgcagttgcatcttctagaactgtacccagtgctggaatccatacaaaagtacttgtggccaagactttgcaagcttgaccaaagaaccttgtatgagctgcactaccagatgataacatttggaaaggtcttctgcacgaaaagcaaaccgaattgcaatgcatgtccaatgagaggagaatgtaggcattttgcaagtgcatttgcaagtgcaagacttgccttgccggggcccgaggagaaaagtatagtgagtgcaactgaagacagaaatacctatcaaaaccctggtgagatcaacaatggaatgcctttgcctctccctctccctcatcctcatcctcatcctcaccgaacagaacagttggaaggaaaccagcaacttgcagcaagccaactatcagaaccaaagtctacactaggttattgtgaaccaattattgaagaaccagctacaccagagccagagtgcacacagatagtagaggacattgaggacttctatgaggacccagatgaaattcctacaattaaacttaacatggaacagttcactcagaacttgcaaaattatatgcaacaaaacatggaacttcaagaaggagaaatgtcaaaggctttagttgctctaacaccagatgctgcatctcttcctacaccgaagcttaagaatgtgagccgactacgaactgagcaccaagtctatgaacttcctgattcacaccctcttctagatcggttggggttggataagcgagaacctgatgatccatgcaattaccttttggccatttggacaccaggtgaaacagcaaactccattcaaccaccagagaataggtgtagttctcaagaatttggcaaattgtgtgatgataaggaatgtttccaatgcaatagtgcacgggaagcatattcacaaacagttcgagggactctcttgataccatgccgaacagcaatgagagggagcttcccacttaacggcacctactttcaagttaatgaggtgtttgctgatcatgattcaagccttgagcctcttgatgttccaagaggctggttgtggaatctgaataggagaactgtgtactttggaacctcaataccaacaatattcaaagggttaacaacaccagaaattcaacattgcttttggagaggatttgtgtgtgtgagggggtttgatcagaaatcaaggggtccgcgtcctttaatggctcgattgcacttcccagccagcaggttggctaaaccaaaagacaagaaagaggagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003906 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016458 GO:0016787 GO:0016798 GO:0016799 GO:0019104 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031935 GO:0031936 GO:0032259 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035510 GO:0040029 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045815 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0080111 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1721

Amino Acids

193.66

Weight (kDa)

6.14

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1335
Acc36I ACCTGC 2 cut(s) 3316, 5121
Acc65I GGTACC 1 cut(s) 3859
AccB1I GGYRCC 4 cut(s) 191, 1769, 3859, 4874
AccB7I CCANNNNNTGG 1 cut(s) 1697
AccBSI CCGCTC 1 cut(s) 2221
AccI GTMKAC 2 cut(s) 2877, 4301
AccII CGCG 3 cut(s) 2241, 3638, 5095
AciI CCGC 7 cut(s) 258, 413, 629, 2221, 2871, 3638, 5093
AcoI YGGCCR 5 cut(s) 370, 2096, 2643, 3944, 4674
AcuI CTGAAG 5 cut(s) 842, 1331, 2426, 3851, 4170
AdeI CACNNNGTG 4 cut(s) 1104, 2507, 2767, 4691
AflII CTTAAG 1 cut(s) 4553
AflIII ACRYGT 1 cut(s) 2822
AhlI ACTAGT 1 cut(s) 2588
AjiI CACGTC 1 cut(s) 1765
AjnI CCWGG 8 cut(s) 240, 552, 573, 874, 2994, 3457, 4174, 4687
AjuI GAANNNNNNNTTGG 4 cut(s) 1842, 1874, 2009, 2041
AleI CACNNNNGTG 1 cut(s) 1763
Alw21I GWGCWC 6 cut(s) 1192, 1656, 3062, 4361, 4584, 4792
Alw26I GTCTC 8 cut(s) 353, 1522, 1682, 2120, 2795, 2888, 3270, 3353
Alw44I GTGCAC 2 cut(s) 4357, 4788
AlwNI CAGNNNCTG 3 cut(s) 512, 3155, 4529
Ama87I CYCGRG 4 cut(s) 206, 356, 3878, 4121
ApaI GGGCCC 1 cut(s) 4122
ApaLI GTGCAC 2 cut(s) 4357, 4788
AseI ATTAAT 1 cut(s) 990
Asp700I GAANNNNTTC 3 cut(s) 991, 1035, 1120
Asp718I GGTACC 1 cut(s) 3859
AspLEI GCGC 3 cut(s) 269, 1665, 2243
AsuC2I CCSGG 2 cut(s) 1863, 4116
AsuHPI GGTGA 7 cut(s) 317, 1242, 1244, 1672, 4190, 4229, 4703
AsuII TTCGAA 1 cut(s) 3072
AvaI CYCGRG 4 cut(s) 206, 356, 3878, 4121
AvaII GGWCC 7 cut(s) 182, 2652, 2842, 2998, 3383, 4396, 5090
BaeGI GKGCMC 3 cut(s) 4122, 4361, 4792
BaeI ACNNNNGTAYC 4 cut(s) 2040, 2073, 3813, 3846
BalI TGGCCA 4 cut(s) 372, 2645, 3946, 4676
BamHI GGATCC 1 cut(s) 1316
BanI GGYRCC 4 cut(s) 191, 1769, 3859, 4874
BanII GRGCYC 1 cut(s) 4122
BarI GAAGNNNNNNTAC 2 cut(s) 1795, 1827
BauI CACGAG 1 cut(s) 3354
BbsI GAAGAC 6 cut(s) 2706, 2895, 3123, 3460, 4009, 4158
Bbv12I GWGCWC 6 cut(s) 1192, 1656, 3062, 4361, 4584, 4792
BbvCI CCTCAGC 1 cut(s) 3312
BccI CCATC 7 cut(s) 1009, 1223, 1691, 2039, 2146, 2174, 3849
BceAI ACGGC 3 cut(s) 2848, 3863, 4888
BcgI CGANNNNNNTGC 4 cut(s) 187, 221, 2364, 2398
BciT130I CCWGG 8 cut(s) 242, 554, 575, 876, 2996, 3459, 4176, 4689
BclI TGATCA 5 cut(s) 13, 1927, 3196, 4906, 5074
BcnI CCSGG 2 cut(s) 1863, 4116
BcoDI GTCTC 8 cut(s) 353, 1522, 1682, 2120, 2795, 2888, 3270, 3353
BcuI ACTAGT 1 cut(s) 2588
BfmI CTRYAG 6 cut(s) 516, 895, 1164, 1273, 2878, 3887
BfrI CTTAAG 1 cut(s) 4553
BfuAI ACCTGC 2 cut(s) 3316, 5121
BglII AGATCT 2 cut(s) 235, 899
BlpI GCTNAGC 2 cut(s) 2452, 3743
BmcAI AGTACT 1 cut(s) 3938
Bme18I GGWCC 7 cut(s) 182, 2652, 2842, 2998, 3383, 4396, 5090
BmeT110I CYCGRG 4 cut(s) 206, 356, 3878, 4121
BmgBI CACGTC 1 cut(s) 1765
BmrI ACTGGG 4 cut(s) 3493, 3571, 3767, 3908
BmuI ACTGGG 4 cut(s) 3493, 3571, 3767, 3908
BoxI GACNNNNGTC 1 cut(s) 3388
BpiI GAAGAC 6 cut(s) 2706, 2895, 3123, 3460, 4009, 4158
BplI GAGNNNNNCTC 4 cut(s) 2780, 2812, 4809, 4841
BpmI CTGGAG 3 cut(s) 557, 708, 1077
Bpu10I CCTNAGC 1 cut(s) 3312
Bpu1102I GCTNAGC 2 cut(s) 2452, 3743
Bpu14I TTCGAA 1 cut(s) 3072
BpuEI CTTGAG 4 cut(s) 81, 2725, 4722, 4943
BpuMI CCSGG 2 cut(s) 1863, 4116
BsaI GGTCTC 2 cut(s) 353, 3353
BsaJI CCNNGG 9 cut(s) 663, 1253, 2233, 2297, 2994, 3751, 4115, 4122, 4174
BsaWI WCCGGW 1 cut(s) 3814
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 7 cut(s) 44, 1815, 3488, 3566, 3672, 3762, 3914
Bse3DI GCAATG 6 cut(s) 51, 2575, 3594, 4051, 4854, 5035
BseBI CCWGG 8 cut(s) 242, 554, 575, 876, 2996, 3459, 4176, 4689
BseDI CCNNGG 9 cut(s) 663, 1253, 2233, 2297, 2994, 3751, 4115, 4122, 4174
BseMI GCAATG 6 cut(s) 51, 2575, 3594, 4051, 4854, 5035
BseNI ACTGG 7 cut(s) 44, 1815, 3488, 3566, 3672, 3762, 3914
BseRI GAGGAG 6 cut(s) 661, 765, 3000, 3329, 4077, 4139
BseSI GKGCMC 3 cut(s) 4122, 4361, 4792
BseYI CCCAGC 3 cut(s) 484, 3850, 5122
BsgI GTGCAG 4 cut(s) 992, 1148, 3971, 4006
Bsh1236I CGCG 3 cut(s) 2241, 3638, 5095
Bsh1285I CGRYCG 1 cut(s) 3819
BshNI GGYRCC 4 cut(s) 191, 1769, 3859, 4874
BsiEI CGRYCG 1 cut(s) 3819
BsiHKAI GWGCWC 6 cut(s) 1192, 1656, 3062, 4361, 4584, 4792
BsiHKCI CYCGRG 4 cut(s) 206, 356, 3878, 4121
BsiSI CCGG 4 cut(s) 195, 1863, 3815, 4115
BslFI GGGAC 3 cut(s) 3396, 3500, 4834
BsmAI GTCTC 8 cut(s) 353, 1522, 1682, 2120, 2795, 2888, 3270, 3353
BsmBI CGTCTC 1 cut(s) 2120
BsmFI GGGAC 3 cut(s) 3396, 3500, 4834
BsmI GAATGC 5 cut(s) 457, 757, 2268, 2375, 4200
Bso31I GGTCTC 2 cut(s) 353, 3353
BsoBI CYCGRG 4 cut(s) 206, 356, 3878, 4121
Bsp119I TTCGAA 1 cut(s) 3072
Bsp120I GGGCCC 1 cut(s) 4118
Bsp1286I GDGCHC 7 cut(s) 1192, 1656, 3062, 4122, 4361, 4584, 4792
Bsp1407I TGTACA 1 cut(s) 1213
Bsp1720I GCTNAGC 2 cut(s) 2452, 3743
Bsp19I CCATGG 1 cut(s) 2297
BspACI CCGC 7 cut(s) 258, 413, 629, 2221, 2871, 3638, 5093
BspFNI CGCG 3 cut(s) 2241, 3638, 5095
BspHI TCATGA 2 cut(s) 2908, 4909
BspMAI CTGCAG 4 cut(s) 899, 1168, 1277, 3891
BspMI ACCTGC 2 cut(s) 3316, 5121
BspQI GCTCTTC 2 cut(s) 435, 2211
BspT104I TTCGAA 1 cut(s) 3072
BspT107I GGYRCC 4 cut(s) 191, 1769, 3859, 4874
BspTI CTTAAG 1 cut(s) 4553
BspTNI GGTCTC 2 cut(s) 353, 3353
BsrBI CCGCTC 1 cut(s) 2221
BsrDI GCAATG 6 cut(s) 51, 2575, 3594, 4051, 4854, 5035
BsrFI RCCGGY 1 cut(s) 194
BsrGI TGTACA 1 cut(s) 1213
BsrI ACTGG 7 cut(s) 44, 1815, 3488, 3566, 3672, 3762, 3914
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 9 cut(s) 663, 1253, 2233, 2297, 2994, 3751, 4115, 4122, 4174
BssSI CACGAG 1 cut(s) 3354
BssT1I CCWWGG 4 cut(s) 663, 1253, 2233, 2297
Bst2BI CACGAG 1 cut(s) 3354
Bst2UI CCWGG 8 cut(s) 242, 554, 575, 876, 2996, 3459, 4176, 4689
Bst6I CTCTTC 9 cut(s) 435, 1252, 1448, 2035, 2118, 2211, 3069, 4542, 4620
BstAFI CTTAAG 1 cut(s) 4553
BstAPI GCANNNNNTGC 5 cut(s) 512, 1660, 2272, 3155, 4106
BstAUI TGTACA 1 cut(s) 1213
BstBI TTCGAA 1 cut(s) 3072
BstDSI CCRYGG 1 cut(s) 2297
BstFNI CGCG 3 cut(s) 2241, 3638, 5095
BstHHI GCGC 3 cut(s) 269, 1665, 2243
BstMAI GTCTC 8 cut(s) 353, 1522, 1682, 2120, 2795, 2888, 3270, 3353
BstMCI CGRYCG 1 cut(s) 3819
BstNI CCWGG 8 cut(s) 242, 554, 575, 876, 2996, 3459, 4176, 4689
BstNSI RCATGY 5 cut(s) 533, 1743, 1896, 2826, 4053
BstPAI GACNNNNGTC 1 cut(s) 3388
BstSFI CTRYAG 6 cut(s) 516, 895, 1164, 1273, 2878, 3887
BstSLI GKGCMC 3 cut(s) 4122, 4361, 4792
BstUI CGCG 3 cut(s) 2241, 3638, 5095
BstV2I GAAGAC 6 cut(s) 2706, 2895, 3123, 3460, 4009, 4158
BstX2I RGATCY 5 cut(s) 235, 899, 1241, 1316, 1354
BstXI CCANNNNNNTGG 3 cut(s) 2947, 4947, 5134
BstYI RGATCY 5 cut(s) 235, 899, 1241, 1316, 1354
BtgI CCRYGG 1 cut(s) 2297
BtrI CACGTC 1 cut(s) 1765
BtsI GCAGTG 4 cut(s) 692, 1073, 1161, 3884
BveI ACCTGC 2 cut(s) 3316, 5121
CaiI CAGNNNCTG 3 cut(s) 512, 3155, 4529
CciI TCATGA 2 cut(s) 2908, 4909
CfoI GCGC 3 cut(s) 269, 1665, 2243
Cfr10I RCCGGY 1 cut(s) 194
CseI GACGC 3 cut(s) 1585, 2295, 5084
CsiI ACCWGGT 1 cut(s) 4687
DraIII CACNNNGTG 4 cut(s) 1104, 2507, 2767, 4691
EaeI YGGCCR 5 cut(s) 370, 2096, 2643, 3944, 4674
Eam1104I CTCTTC 9 cut(s) 435, 1252, 1448, 2035, 2118, 2211, 3069, 4542, 4620
EarI CTCTTC 9 cut(s) 435, 1252, 1448, 2035, 2118, 2211, 3069, 4542, 4620
Eco130I CCWWGG 4 cut(s) 663, 1253, 2233, 2297
Eco147I AGGCCT 1 cut(s) 76
Eco24I GRGCYC 1 cut(s) 4122
Eco31I GGTCTC 2 cut(s) 353, 3353
Eco47I GGWCC 7 cut(s) 182, 2652, 2842, 2998, 3383, 4396, 5090
Eco57I CTGAAG 5 cut(s) 842, 1331, 2426, 3851, 4170
Eco88I CYCGRG 4 cut(s) 206, 356, 3878, 4121
EcoO109I RGGNCCY 3 cut(s) 2998, 4118, 4396
EcoRI GAATTC 1 cut(s) 3653
EcoRII CCWGG 8 cut(s) 240, 552, 573, 874, 2994, 3457, 4174, 4687
EcoT14I CCWWGG 4 cut(s) 663, 1253, 2233, 2297
EcoT22I ATGCAT 8 cut(s) 294, 1431, 1745, 2268, 2431, 2948, 3378, 4051
EcoT38I GRGCYC 1 cut(s) 4122
ErhI CCWWGG 4 cut(s) 663, 1253, 2233, 2297
Esp3I CGTCTC 1 cut(s) 2120
FalI AAGNNNNNCTT 2 cut(s) 2100, 2132
FaqI GGGAC 3 cut(s) 3396, 3500, 4834
FauNDI CATATG 3 cut(s) 1405, 2898, 2942
FbaI TGATCA 5 cut(s) 13, 1927, 3196, 4906, 5074
FblI GTMKAC 2 cut(s) 2877, 4301
FriOI GRGCYC 1 cut(s) 4122
GlaI GCGC 3 cut(s) 268, 1664, 2242
GsaI CCCAGC 3 cut(s) 488, 3854, 5126
GsuI CTGGAG 3 cut(s) 557, 708, 1077
HapII CCGG 4 cut(s) 195, 1863, 3815, 4115
HgaI GACGC 3 cut(s) 1585, 2295, 5084
HhaI GCGC 3 cut(s) 269, 1665, 2243
Hin6I GCGC 3 cut(s) 267, 1663, 2241
HinP1I GCGC 3 cut(s) 267, 1663, 2241
HincII GTYRAC 3 cut(s) 2113, 2563, 5016
HindII GTYRAC 3 cut(s) 2113, 2563, 5016
HindIII AAGCTT 5 cut(s) 2627, 3182, 3400, 3959, 4550
HpaI GTTAAC 1 cut(s) 5016
HpaII CCGG 4 cut(s) 195, 1863, 3815, 4115
HphI GGTGA 7 cut(s) 317, 1242, 1244, 1672, 4190, 4229, 4703
Hpy99I CGWCG 1 cut(s) 2097
HpyCH4IV ACGT 5 cut(s) 1714, 1764, 2092, 2395, 3835
HpySE526I ACGT 5 cut(s) 1714, 1764, 2092, 2395, 3835
HspAI GCGC 3 cut(s) 267, 1663, 2241
KpnI GGTACC 1 cut(s) 3863
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
Ksp22I TGATCA 5 cut(s) 13, 1927, 3196, 4906, 5074
KspAI GTTAAC 1 cut(s) 5016
LguI GCTCTTC 2 cut(s) 435, 2211
LmnI GCTCC 9 cut(s) 61, 176, 576, 1612, 2950, 2987, 3316, 3684, 4857
MabI ACCWGGT 1 cut(s) 4687
MaeII ACGT 5 cut(s) 1714, 1764, 2092, 2395, 3835
MbiI CCGCTC 1 cut(s) 2221
MfeI CAATTG 1 cut(s) 3232
MflI RGATCY 5 cut(s) 235, 899, 1241, 1316, 1354
MhlI GDGCHC 7 cut(s) 1192, 1656, 3062, 4122, 4361, 4584, 4792
MlsI TGGCCA 4 cut(s) 372, 2645, 3946, 4676
MluNI TGGCCA 4 cut(s) 372, 2645, 3946, 4676
MmeI TCCRAC 7 cut(s) 83, 1206, 1293, 3057, 3811, 4233, 4614
Mox20I TGGCCA 4 cut(s) 372, 2645, 3946, 4676
Mph1103I ATGCAT 8 cut(s) 294, 1431, 1745, 2268, 2431, 2948, 3378, 4051
MroNI GCCGGC 1 cut(s) 194
MroXI GAANNNNTTC 3 cut(s) 991, 1035, 1120
MscI TGGCCA 4 cut(s) 372, 2645, 3946, 4676
MslI CAYNNNNRTG 6 cut(s) 1013, 1206, 1763, 2683, 2945, 3211
Msp20I TGGCCA 4 cut(s) 372, 2645, 3946, 4676
MspA1I CMGCKG 3 cut(s) 272, 634, 1694
MspCI CTTAAG 1 cut(s) 4553
MspI CCGG 4 cut(s) 195, 1863, 3815, 4115
MunI CAATTG 1 cut(s) 3232
Mva1269I GAATGC 5 cut(s) 457, 757, 2268, 2375, 4200
MvaI CCWGG 8 cut(s) 242, 554, 575, 876, 2996, 3459, 4176, 4689
MvnI CGCG 3 cut(s) 2241, 3638, 5095
NaeI GCCGGC 1 cut(s) 196
NciI CCSGG 2 cut(s) 1863, 4116
NcoI CCATGG 1 cut(s) 2297
NdeI CATATG 3 cut(s) 1405, 2898, 2942
NgoMIV GCCGGC 1 cut(s) 194
NmuCI GTSAC 7 cut(s) 1071, 1199, 1232, 1504, 1622, 1756, 3882
NsiI ATGCAT 8 cut(s) 294, 1431, 1745, 2268, 2431, 2948, 3378, 4051
NspI RCATGY 5 cut(s) 533, 1743, 1896, 2826, 4053
NspV TTCGAA 1 cut(s) 3072
OliI CACNNNNGTG 1 cut(s) 1763
PaeI GCATGC 1 cut(s) 1896
PaeR7I CTCGAG 1 cut(s) 206
PagI TCATGA 2 cut(s) 2908, 4909
PceI AGGCCT 1 cut(s) 76
PciI ACATGT 1 cut(s) 2822
PciSI GCTCTTC 2 cut(s) 435, 2211
PcsI WCGNNNNNNNCGW 1 cut(s) 2098
PctI GAATGC 5 cut(s) 457, 757, 2268, 2375, 4200
PdiI GCCGGC 1 cut(s) 196
PdmI GAANNNNTTC 3 cut(s) 991, 1035, 1120
PflFI GACNNNGTC 1 cut(s) 3759
PflMI CCANNNNNTGG 1 cut(s) 1697
PfoI TCCNGGA 3 cut(s) 552, 573, 1861
PpuMI RGGWCCY 2 cut(s) 2998, 4396
PscI ACATGT 1 cut(s) 2822
PshAI GACNNNNGTC 1 cut(s) 3388
PshBI ATTAAT 1 cut(s) 990
PsiI TTATAA 1 cut(s) 1335
Psp5II RGGWCCY 2 cut(s) 2998, 4396
Psp6I CCWGG 8 cut(s) 240, 552, 573, 874, 2994, 3457, 4174, 4687
PspFI CCCAGC 3 cut(s) 484, 3850, 5122
PspGI CCWGG 8 cut(s) 240, 552, 573, 874, 2994, 3457, 4174, 4687
PspOMI GGGCCC 1 cut(s) 4118
PspPPI RGGWCCY 2 cut(s) 2998, 4396
PstI CTGCAG 4 cut(s) 899, 1168, 1277, 3891
PstNI CAGNNNCTG 3 cut(s) 512, 3155, 4529
PsuI RGATCY 5 cut(s) 235, 899, 1241, 1316, 1354
PsyI GACNNNGTC 1 cut(s) 3759
PvuII CAGCTG 3 cut(s) 272, 634, 1694
RseI CAYNNNNRTG 6 cut(s) 1013, 1206, 1763, 2683, 2945, 3211
SapI GCTCTTC 2 cut(s) 435, 2211
ScaI AGTACT 1 cut(s) 3938
SduI GDGCHC 7 cut(s) 1192, 1656, 3062, 4122, 4361, 4584, 4792
SexAI ACCWGGT 1 cut(s) 4687
SfcI CTRYAG 6 cut(s) 516, 895, 1164, 1273, 2878, 3887
Sfr274I CTCGAG 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 3072
SinI GGWCC 7 cut(s) 182, 2652, 2842, 2998, 3383, 4396, 5090
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 6 cut(s) 1013, 1206, 1763, 2683, 2945, 3211
SmlI CTYRAG 6 cut(s) 96, 206, 2704, 4553, 4737, 4922
SmoI CTYRAG 6 cut(s) 96, 206, 2704, 4553, 4737, 4922
SpeI ACTAGT 1 cut(s) 2588
SphI GCATGC 1 cut(s) 1896
SseBI AGGCCT 1 cut(s) 76
SsiI CCGC 7 cut(s) 258, 413, 629, 2221, 2871, 3638, 5093
SspI AATATT 3 cut(s) 1331, 3097, 5004
StuI AGGCCT 1 cut(s) 76
StyI CCWWGG 4 cut(s) 663, 1253, 2233, 2297
TaiI ACGT 5 cut(s) 1717, 1767, 2095, 2398, 3838
TaqI TCGA 6 cut(s) 207, 215, 3072, 3819, 4816, 5110
TatI WGTACW 3 cut(s) 1213, 3936, 4977
TauI GCSGC 2 cut(s) 260, 2873
TseFI GTSAC 7 cut(s) 1071, 1199, 1232, 1504, 1622, 1756, 3882
Tsp45I GTSAC 7 cut(s) 1071, 1199, 1232, 1504, 1622, 1756, 3882
TspGWI ACGGA 4 cut(s) 1114, 1900, 2144, 2509
Tth111I GACNNNGTC 1 cut(s) 3759
Van91I CCANNNNNTGG 1 cut(s) 1697
Vha464I CTTAAG 1 cut(s) 4553
VneI GTGCAC 2 cut(s) 4357, 4788
VpaK11BI GGWCC 7 cut(s) 182, 2652, 2842, 2998, 3383, 4396, 5090
VspI ATTAAT 1 cut(s) 990
XbaI TCTAGA 2 cut(s) 3901, 4618
XceI RCATGY 5 cut(s) 533, 1743, 1896, 2826, 4053
XhoI CTCGAG 1 cut(s) 206
XmiI GTMKAC 2 cut(s) 2877, 4301
XmnI GAANNNNTTC 3 cut(s) 991, 1035, 1120
ZrmI AGTACT 1 cut(s) 3938
Zsp2I ATGCAT 8 cut(s) 294, 1431, 1745, 2268, 2431, 2948, 3378, 4051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.