pycom14g01290

Permuted single zf-CXXC unit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002894
Physical Location & Seq
Forward (+)
929478 .. 933855
4378 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g01290.5

Sequence Viewer

Length: 2895 bp
ATGTTCCTTGTAAAGAGCTCATCGATCCTTGCGGTGGCCTCTGCAAATGACGGGAGCGGTGGTGCAGTGCTTGGAGATACTCAGCATGAATTTTTGTTCCCTGACAACCATCCTTATGATCTGAATGCTCCACCTCCGACTACATATGGCCAATTTGCACCTATAACACCGGATAAGTCCATGAGACTAGACAGTGTTCAAATGTCTCAAATTCCGAGCTCCAACGCAGATGATGGACGAGGCCAGGAGATAGAAGAGCAATGGGATGCTAATTCTGCGACAATTAACATAATTGATCTGGAAAACAATAAAGATATAGAAAAATCTGCCGTGGATTCATCACAAGCTACTTTCTCCACGCAGCTCCAGGAACATTGCAACCCTGACAAGGAGGTGAACATCATTATAGACTTGAACAATACACCACAACCGAAACAAAGAAGGAGAAAGCACAGGCCGAAGGTGATTATAGAAGGCAAGCTGAAAAGAAACCCGCAGCCTTCTGGTTCTATGGAAAATCCAAAACCCAAACGTAAGTATGTGCGGAAGAGTACACTCGAGAAAAACAAAACTCCTCCACCAAAGGAATCTACAGAGCATATTGATTCAAATAATGTGAAGCCTCCGAAGAGATCATGCAGAAGGACTTTAAACTATGCCGACACGGAAGAACCAATAGATGGAATTTCCTCGAGCAAGTCTTTCAATGTGGGTTCACAATCACAGGAGATGAATGTTGGCACCGATGGAGTTCAATCAAATTCAACTGGTCCGTGCAGGAATGAAGTTGAATTGGTGGCAGATAACACCCAAGCAGGCATAGCCCAAGATCTCGTCCGTTCTACAAGCCGAATGCTGAAACATTACTTGTCATTGCCGGACCAGCAACCTCCAATCACACCACAACAAATTAGAGGTTCTACTACATATGTTAATTCCCAAAAGGAGGCTGCAGAAGAAATAGGACAAGTGAGCACTTGTAGTGGACAGGCAAACCCTGCACAAACCATGTTGGATTCTATTCGATCATCCCAACGAAGTGCAAATGATTCCACCTGCAGCACAAATACAGTTCTGGCAACAGAAGAAGAGCAAGCAAAAAGATCAAAGAGAAACTATTCGAATGCTGTTGAGCAAGCAGATCCTAGAACCGGAAATTTGTTGGGGGCTAACTATAATAATTTGCCGGCATACTACAATGTAATGTCCTGGGTGCATTTTCCATACATATACAAGAAGAAGAGGACTGACAAGGCGCAAAATTCTACCATACCAAGCACCTCATATCGTGTGACTATGGCAGAAAATGTATGGAGACCATCGACAGCTGGTTACTTGACTTCTGGTCCTCAAGTGAATGCTGATAATTTTTCAACTGCACTTGGAGAAGCAGGAAAAACCCCTCAGGAGAGACCGCAAGGTGTTCACAGTTTCCTGCCTTTGTATCAGACAGAGAGGTCAACAAAGAGAAGATCTAGTTGCCCTACAAGGGTCCGTGACTTAGCTTCGCTAACCAGAACACCAGAGCACATTCTGCACAGAACCTGTCTCGTCAAACAACCACCCACTGACGGCAATGGTCAAAGAGTGAACCACTTTGACAAATCTCAGACATGCATTGACGCACTAGTGACAGACGTCGGTGCAACACTCGCAAAAAAGAAGAGGACAAAGAGAAATCCACTTTCCAGTTCACAACGAGGTCTTGTAATCTATCAGAACCAGCAATCTTTCGCCACTGCATCAGGTGTTCCTCCAGAAGTACCATTTGAACAACTGCTTTCTGCTATTACTGAGCACTTAAAATGTTTGAACATAAACAGAGAAAATAGCAGCAGATTTGCCTATCATGGGTATAATGTGATCTCTTCTTATAAGGCACAATATCAAGAGCACAATGCACTTGTTCTTTATAGAAGAGATGGCACTGTTGTACCATTTGATGGTTCGTTTGATCCAATTAAGAAACGTAAAGCGCGACCTAAAGTTGATATTGATCAAGAGACTGATAGAGTATGGAAGCTTCTGCTGGAAAATATAAATAGTGAAGGTATTAATGGAACAGACCAGGAGAAAGAAAAATGGTGGGAAGAAGAGCGTAGAGTGTTCCATGGACGTGCAGACAATTTTATTGCACGAATGCATCTTGTGCAAGGTGATAGACGCTTCTCTCCGTGGAAGGGATCAGTTCTGGACTCAGTGGTTGGAGTTTTCCTTACACAAAATGTCTCAGACCACCTCTCTAGCTCTGCATTCATGTCAATGGCTGCACATTTTCCCCTGAAGTTGAGGAGCAACGAAAAATCATGTGATGAAGAGGTTGCGAGTTTGGTAGTAGATGAACCAGAAGTGTGCATTAGTGAGAATTCAAACCAGCCAGGTTGCGACTGGAGTTCCCTCACATTCCATGACGCTGAGCATAGTGAAGAAAAAGTTGTCAATGGAAACGAGAACTCTGGAAGCACTACTGAGGGGGTTATCTCAACAAATGAAGCAGAATGCAAGCTATCACATCCATCTGAATCTGGTCCGGGGTTGTACCCTAACTCATTAATGAACAGGTCAACCACCAAGATCACAAGAACAGGATCGTACCTGCAGGAGGATATGAGAACTTATGATGTAGTTTCATCTCAAAATTCTGTGGATTCTTCAACTTCACAAACTGTGGAAAAGACAGGATCATGCGAGAGCAACTCAGAAACAGAGTATCCACCTAATAGATGTGAAAACAGCAGTTTAGATCACTCCACGTCATTTGTGGAACTTTTACAGAGGGCCGAATCAAGTATGCTGCATTACAGTCTCAAGGGCACCCATATGTCCTCTCATGACACATCAAACTGTGGAGGTTACCAACCTGCATGCATGCAGCATAATCGATCAAAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003906 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016458 GO:0016787 GO:0016798 GO:0016799 GO:0019104 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031935 GO:0031936 GO:0032259 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035510 GO:0040029 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045815 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0080111 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

965

Amino Acids

107.22

Weight (kDa)

6.54

Isoelectric Point (pI)

57.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1875
AarI CACCTGC 1 cut(s) 1064
AasI GACNNNNNNGTC 1 cut(s) 1456
AatII GACGTC 1 cut(s) 1641
Acc36I ACCTGC 3 cut(s) 1064, 2604, 2869
AccB1I GGYRCC 2 cut(s) 740, 2813
AccB7I CCANNNNNTGG 2 cut(s) 680, 1943
AccBSI CCGCTC 1 cut(s) 57
AccII CGCG 1 cut(s) 1978
AciI CCGC 5 cut(s) 32, 57, 494, 544, 1415
AclWI GGATC 6 cut(s) 19, 1136, 1949, 2191, 2596, 2689
AcoI YGGCCR 1 cut(s) 148
AcsI RAATTY 8 cut(s) 89, 210, 684, 760, 1156, 1261, 2365, 2638
AcuI CTGAAG 1 cut(s) 2303
AcyI GRCGYC 1 cut(s) 1638
AfaI GTAC 5 cut(s) 553, 1764, 1935, 2540, 2594
AhdI GACNNNNNGTC 1 cut(s) 1344
AhlI ACTAGT 1 cut(s) 1627
AjiI CACGTC 3 cut(s) 2117, 2754, 2892
AjnI CCWGG 5 cut(s) 243, 366, 1208, 2067, 2377
Alw21I GWGCWC 6 cut(s) 20, 221, 977, 1530, 1800, 1896
Alw26I GTCTC 8 cut(s) 178, 210, 1309, 1405, 1553, 1997, 2233, 2810
AlwI GGATC 6 cut(s) 19, 1136, 1949, 2191, 2596, 2689
AlwNI CAGNNNCTG 1 cut(s) 1545
Ama87I CYCGRG 2 cut(s) 557, 691
AoxI GGCC 5 cut(s) 36, 148, 241, 455, 2778
ApeKI GCWGC 8 cut(s) 361, 496, 950, 1059, 1833, 2267, 2794, 2872
ApoI RAATTY 8 cut(s) 89, 210, 684, 760, 1156, 1261, 2365, 2638
AseI ATTAAT 2 cut(s) 2055, 2552
Asp700I GAANNNNTTC 1 cut(s) 1117
AspLEI GCGC 2 cut(s) 1258, 1978
AspS9I GGNCC 6 cut(s) 770, 880, 1346, 1492, 2528, 2778
AsuC2I CCSGG 1 cut(s) 2532
AsuHPI GGTGA 3 cut(s) 406, 475, 2168
AsuII TTCGAA 1 cut(s) 1121
AvaI CYCGRG 2 cut(s) 557, 691
AvaII GGWCC 5 cut(s) 770, 880, 1346, 1492, 2528
AxyI CCTNAGG 1 cut(s) 1404
BaeGI GKGCMC 1 cut(s) 2816
BaeI ACNNNNGTAYC 2 cut(s) 1917, 1950
BalI TGGCCA 1 cut(s) 150
BanI GGYRCC 2 cut(s) 740, 2813
BanII GRGCYC 2 cut(s) 20, 221
BarI GAAGNNNNNNTAC 2 cut(s) 2007, 2039
Bbv12I GWGCWC 6 cut(s) 20, 221, 977, 1530, 1800, 1896
BbvI GCAGC 8 cut(s) 373, 508, 937, 1071, 1845, 2254, 2781, 2884
BccI CCATC 8 cut(s) 117, 227, 674, 740, 1327, 1916, 1937, 2524
BceAI ACGGC 2 cut(s) 314, 1588
BcgI CGANNNNNNTGC 2 cut(s) 2861, 2895
BciT130I CCWGG 5 cut(s) 245, 368, 1210, 2069, 2379
BciVI GTATCC 1 cut(s) 2721
BclI TGATCA 1 cut(s) 1996
BcnI CCSGG 1 cut(s) 2532
BcoDI GTCTC 8 cut(s) 178, 210, 1309, 1405, 1553, 1997, 2233, 2810
BcuI ACTAGT 1 cut(s) 1627
BfaI CTAG 5 cut(s) 188, 1146, 1476, 1628, 2244
BfmI CTRYAG 4 cut(s) 591, 951, 1057, 2597
BfuAI ACCTGC 3 cut(s) 1064, 2604, 2869
BfuI GTATCC 1 cut(s) 2721
BglII AGATCT 2 cut(s) 829, 1472
BisI GCNGC 8 cut(s) 362, 497, 951, 1060, 1834, 2268, 2795, 2873
BlpI GCTNAGC 1 cut(s) 2415
BlsI GCNGC 8 cut(s) 363, 498, 952, 1061, 1835, 2269, 2796, 2874
Bme1390I CCNGG 6 cut(s) 245, 368, 1210, 2069, 2379, 2532
Bme18I GGWCC 5 cut(s) 770, 880, 1346, 1492, 2528
BmeRI GACNNNNNGTC 1 cut(s) 1344
BmeT110I CYCGRG 2 cut(s) 557, 691
BmgBI CACGTC 3 cut(s) 2117, 2754, 2892
BmgT120I GGNCC 6 cut(s) 770, 880, 1346, 1492, 2528, 2778
BmiI GGNNCC 3 cut(s) 742, 1493, 2815
BmrFI CCNGG 6 cut(s) 245, 368, 1210, 2069, 2379, 2532
BmsI GCATC 3 cut(s) 256, 1751, 2152
BoxI GACNNNNGTC 1 cut(s) 1637
BplI GAGNNNNNCTC 2 cut(s) 2681, 2713
BpmI CTGGAG 3 cut(s) 350, 1740, 2410
Bpu1102I GCTNAGC 1 cut(s) 2415
Bpu14I TTCGAA 1 cut(s) 1121
BpuEI CTTGAG 2 cut(s) 1335, 2792
BpuMI CCSGG 1 cut(s) 2532
Bsa29I ATCGAT 2 cut(s) 23, 2881
BsaBI GATNNNNATC 1 cut(s) 1995
BsaHI GRCGYC 1 cut(s) 1638
BsaI GGTCTC 2 cut(s) 1309, 1405
BsaJI CCNNGG 5 cut(s) 330, 1209, 2110, 2174, 2531
BsaWI WCCGGW 2 cut(s) 169, 1151
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bse118I RCCGGY 1 cut(s) 1186
Bse1I ACTGG 3 cut(s) 772, 1689, 2393
Bse21I CCTNAGG 1 cut(s) 1404
Bse3DI GCAATG 4 cut(s) 266, 373, 872, 1582
Bse8I GATNNNNATC 1 cut(s) 1995
BseBI CCWGG 5 cut(s) 245, 368, 1210, 2069, 2379
BseCI ATCGAT 2 cut(s) 23, 2881
BseDI CCNNGG 5 cut(s) 330, 1209, 2110, 2174, 2531
BseGI GGATG 4 cut(s) 109, 271, 1028, 2512
BseJI GATNNNNATC 1 cut(s) 1995
BseMI GCAATG 4 cut(s) 266, 373, 872, 1582
BseMII CTCAG 9 cut(s) 95, 1418, 1622, 1785, 2211, 2244, 2406, 2460, 2712
BseNI ACTGG 3 cut(s) 772, 1689, 2393
BseRI GAGGAG 2 cut(s) 564, 2305
BseSI GKGCMC 1 cut(s) 2816
BseXI GCAGC 8 cut(s) 373, 508, 937, 1071, 1845, 2254, 2781, 2884
BsgI GTGCAG 7 cut(s) 84, 796, 984, 1362, 1520, 2139, 2253
Bsh1236I CGCG 1 cut(s) 1978
BshFI GGCC 5 cut(s) 38, 150, 243, 457, 2780
BshNI GGYRCC 2 cut(s) 740, 2813
BshVI ATCGAT 2 cut(s) 23, 2881
BsiHKAI GWGCWC 6 cut(s) 20, 221, 977, 1530, 1800, 1896
BsiHKCI CYCGRG 2 cut(s) 557, 691
BsiSI CCGG 5 cut(s) 170, 878, 1152, 1187, 2531
BsmAI GTCTC 8 cut(s) 178, 210, 1309, 1405, 1553, 1997, 2233, 2810
BsmI GAATGC 7 cut(s) 130, 858, 1129, 1363, 2145, 2252, 2504
BsnI GGCC 5 cut(s) 38, 150, 243, 457, 2780
Bso31I GGTCTC 2 cut(s) 1309, 1405
BsoBI CYCGRG 2 cut(s) 557, 691
Bsp119I TTCGAA 1 cut(s) 1121
Bsp1286I GDGCHC 7 cut(s) 20, 221, 977, 1530, 1800, 1896, 2816
Bsp1720I GCTNAGC 1 cut(s) 2415
Bsp19I CCATGG 1 cut(s) 2110
BspACI CCGC 5 cut(s) 32, 57, 494, 544, 1415
BspANI GGCC 5 cut(s) 38, 150, 243, 457, 2780
BspCNI CTCAG 9 cut(s) 94, 1417, 1621, 1786, 2210, 2243, 2407, 2461, 2711
BspDI ATCGAT 2 cut(s) 23, 2881
BspFNI CGCG 1 cut(s) 1978
BspHI TCATGA 1 cut(s) 2830
BspLI GGNNCC 3 cut(s) 742, 1493, 2815
BspMAI CTGCAG 3 cut(s) 955, 1061, 2601
BspMI ACCTGC 3 cut(s) 1064, 2604, 2869
BspPI GGATC 6 cut(s) 19, 1136, 1949, 2191, 2596, 2689
BspQI GCTCTTC 3 cut(s) 249, 1083, 2088
BspT104I TTCGAA 1 cut(s) 1121
BspT107I GGYRCC 2 cut(s) 740, 2813
BspTNI GGTCTC 2 cut(s) 1309, 1405
BsrBI CCGCTC 1 cut(s) 57
BsrDI GCAATG 4 cut(s) 266, 373, 872, 1582
BsrFI RCCGGY 1 cut(s) 1186
BsrI ACTGG 3 cut(s) 772, 1689, 2393
BssAI RCCGGY 1 cut(s) 1186
BssECI CCNNGG 5 cut(s) 330, 1209, 2110, 2174, 2531
BssNI GRCGYC 1 cut(s) 1638
BssT1I CCWWGG 1 cut(s) 2110
Bst2UI CCWGG 5 cut(s) 245, 368, 1210, 2069, 2379
Bst4CI ACNGT 7 cut(s) 194, 1072, 1430, 1930, 2668, 2804, 2846
BstACI GRCGYC 1 cut(s) 1638
BstAPI GCANNNNNTGC 3 cut(s) 998, 1534, 2149
BstBI TTCGAA 1 cut(s) 1121
BstC8I GCNNGC 8 cut(s) 479, 817, 1095, 1137, 1188, 2504, 2866, 2870
BstDSI CCRYGG 3 cut(s) 330, 2110, 2174
BstEII GGTNACC 1 cut(s) 2852
BstENI CCTNNNNNAGG 1 cut(s) 2600
BstF5I GGATG 4 cut(s) 109, 271, 1028, 2512
BstFNI CGCG 1 cut(s) 1978
BstHHI GCGC 2 cut(s) 1258, 1978
BstMAI GTCTC 8 cut(s) 178, 210, 1309, 1405, 1553, 1997, 2233, 2810
BstMWI GCNNNNNNNGC 7 cut(s) 275, 821, 883, 998, 1534, 1652, 2149
BstNI CCWGG 5 cut(s) 245, 368, 1210, 2069, 2379
BstNSI RCATGY 3 cut(s) 1617, 2868, 2872
BstPAI GACNNNNGTC 1 cut(s) 1637
BstPI GGTNACC 1 cut(s) 2852
BstSCI CCNGG 6 cut(s) 243, 366, 1208, 2067, 2377, 2530
BstSFI CTRYAG 4 cut(s) 591, 951, 1057, 2597
BstSLI GKGCMC 1 cut(s) 2816
BstUI CGCG 1 cut(s) 1978
BstV1I GCAGC 8 cut(s) 373, 508, 937, 1071, 1845, 2254, 2781, 2884
BstX2I RGATCY 3 cut(s) 829, 1141, 1472
BstYI RGATCY 3 cut(s) 829, 1141, 1472
Bsu15I ATCGAT 2 cut(s) 23, 2881
Bsu36I CCTNAGG 1 cut(s) 1404
BsuI GTATCC 1 cut(s) 2721
BsuRI GGCC 5 cut(s) 38, 150, 243, 457, 2780
BsuTUI ATCGAT 2 cut(s) 23, 2881
BtgI CCRYGG 3 cut(s) 330, 2110, 2174
BtrI CACGTC 3 cut(s) 2117, 2754, 2892
BtsCI GGATG 4 cut(s) 109, 271, 1028, 2512
BtsI GCAGTG 2 cut(s) 72, 1737
BtsIMutI CAGTG 6 cut(s) 72, 199, 1566, 1737, 1926, 2205
BveI ACCTGC 3 cut(s) 1064, 2604, 2869
Cac8I GCNNGC 8 cut(s) 479, 817, 1095, 1137, 1188, 2504, 2866, 2870
CaiI CAGNNNCTG 1 cut(s) 1545
CciI TCATGA 1 cut(s) 2830
CfoI GCGC 2 cut(s) 1258, 1978
Cfr10I RCCGGY 1 cut(s) 1186
Cfr13I GGNCC 6 cut(s) 770, 880, 1346, 1492, 2528, 2778
ClaI ATCGAT 2 cut(s) 23, 2881
CseI GACGC 3 cut(s) 1631, 2172, 2420
Csp6I GTAC 5 cut(s) 552, 1763, 1934, 2539, 2593
CspCI CAANNNNNGTGG 2 cut(s) 2740, 2775
CviQI GTAC 5 cut(s) 552, 1763, 1934, 2539, 2593
DraI TTTAAA 1 cut(s) 651
DrdI GACNNNNNNGTC 1 cut(s) 1456
DriI GACNNNNNGTC 1 cut(s) 1344
DseDI GACNNNNNNGTC 1 cut(s) 1456
EaeI YGGCCR 1 cut(s) 148
Eam1105I GACNNNNNGTC 1 cut(s) 1344
Ecl136II GAGCTC 2 cut(s) 18, 219
Eco130I CCWWGG 1 cut(s) 2110
Eco24I GRGCYC 2 cut(s) 20, 221
Eco31I GGTCTC 2 cut(s) 1309, 1405
Eco47I GGWCC 5 cut(s) 770, 880, 1346, 1492, 2528
Eco53kI GAGCTC 2 cut(s) 18, 219
Eco57I CTGAAG 1 cut(s) 2303
Eco81I CCTNAGG 1 cut(s) 1404
Eco88I CYCGRG 2 cut(s) 557, 691
Eco91I GGTNACC 1 cut(s) 2852
EcoICRI GAGCTC 2 cut(s) 18, 219
EcoNI CCTNNNNNAGG 1 cut(s) 2600
EcoO65I GGTNACC 1 cut(s) 2852
EcoRI GAATTC 1 cut(s) 2365
EcoRII CCWGG 5 cut(s) 243, 366, 1208, 2067, 2377
EcoT14I CCWWGG 1 cut(s) 2110
EcoT22I ATGCAT 3 cut(s) 1619, 2145, 2870
EcoT38I GRGCYC 2 cut(s) 20, 221
ErhI CCWWGG 1 cut(s) 2110
FauI CCCGC 1 cut(s) 501
FauNDI CATATG 3 cut(s) 145, 928, 2820
FbaI TGATCA 1 cut(s) 1996
Fnu4HI GCNGC 8 cut(s) 362, 497, 951, 1060, 1834, 2268, 2795, 2873
FokI GGATG 4 cut(s) 96, 278, 1015, 2499
FriOI GRGCYC 2 cut(s) 20, 221
Fsp4HI GCNGC 8 cut(s) 362, 497, 951, 1060, 1834, 2268, 2795, 2873
FspBI CTAG 5 cut(s) 188, 1146, 1476, 1628, 2244
GlaI GCGC 2 cut(s) 1257, 1977
GluI GCNGC 8 cut(s) 362, 497, 951, 1060, 1834, 2268, 2795, 2873
GsuI CTGGAG 3 cut(s) 350, 1740, 2410
HaeIII GGCC 5 cut(s) 38, 150, 243, 457, 2780
HapII CCGG 5 cut(s) 170, 878, 1152, 1187, 2531
HgaI GACGC 3 cut(s) 1631, 2172, 2420
HhaI GCGC 2 cut(s) 1258, 1978
Hin1I GRCGYC 1 cut(s) 1638
Hin6I GCGC 2 cut(s) 1256, 1976
HinP1I GCGC 2 cut(s) 1256, 1976
HincII GTYRAC 2 cut(s) 1461, 2565
HindII GTYRAC 2 cut(s) 1461, 2565
HindIII AAGCTT 1 cut(s) 2021
HinfI GANTC 9 cut(s) 335, 587, 605, 1016, 1049, 2195, 2522, 2648, 2783
HpaII CCGG 5 cut(s) 170, 878, 1152, 1187, 2531
HphI GGTGA 3 cut(s) 406, 475, 2168
Hpy166II GTNNAC 9 cut(s) 397, 554, 716, 986, 1426, 1461, 1591, 1694, 2565
Hpy188III TCNNGA 9 cut(s) 299, 559, 1406, 1757, 1889, 2000, 2192, 2457, 2831
Hpy8I GTNNAC 9 cut(s) 397, 554, 716, 986, 1426, 1461, 1591, 1694, 2565
Hpy99I CGWCG 1 cut(s) 1643
HpyAV CCTTC 7 cut(s) 435, 454, 467, 510, 636, 2042, 2173
HpyCH4III ACNGT 7 cut(s) 194, 1072, 1430, 1930, 2668, 2804, 2846
HpyCH4IV ACGT 6 cut(s) 532, 1638, 1969, 2116, 2753, 2891
HpyF10VI GCNNNNNNNGC 7 cut(s) 275, 821, 883, 998, 1534, 1652, 2149
HpySE526I ACGT 6 cut(s) 532, 1638, 1969, 2116, 2753, 2891
Hsp92I GRCGYC 1 cut(s) 1638
HspAI GCGC 2 cut(s) 1256, 1976
KroI GCCGGC 1 cut(s) 1186
KroNI GCCGGC 1 cut(s) 1188
Ksp22I TGATCA 1 cut(s) 1996
LguI GCTCTTC 3 cut(s) 249, 1083, 2088
LmnI GCTCC 5 cut(s) 54, 133, 224, 369, 2292
Lsp1109I GCAGC 8 cut(s) 373, 508, 937, 1071, 1845, 2254, 2781, 2884
LweI GCATC 3 cut(s) 256, 1751, 2152
MaeI CTAG 5 cut(s) 188, 1146, 1476, 1628, 2244
MaeII ACGT 6 cut(s) 532, 1638, 1969, 2116, 2753, 2891
MaeIII GTNAC 5 cut(s) 1291, 1331, 1496, 1630, 2852
MbiI CCGCTC 1 cut(s) 57
MflI RGATCY 3 cut(s) 829, 1141, 1472
MhlI GDGCHC 7 cut(s) 20, 221, 977, 1530, 1800, 1896, 2816
MlsI TGGCCA 1 cut(s) 150
MluNI TGGCCA 1 cut(s) 150
MlyI GAGTC 1 cut(s) 2189
MmeI TCCRAC 4 cut(s) 161, 246, 993, 2185
Mox20I TGGCCA 1 cut(s) 150
Mph1103I ATGCAT 3 cut(s) 1619, 2145, 2870
MroNI GCCGGC 1 cut(s) 1186
MroXI GAANNNNTTC 1 cut(s) 1117
MscI TGGCCA 1 cut(s) 150
MseI TTAA 7 cut(s) 285, 650, 933, 1802, 1962, 2055, 2552
MslI CAYNNNNRTG 4 cut(s) 114, 2115, 2261, 2819
Msp20I TGGCCA 1 cut(s) 150
MspA1I CMGCKG 1 cut(s) 1328
MspI CCGG 5 cut(s) 170, 878, 1152, 1187, 2531
MspR9I CCNGG 6 cut(s) 245, 368, 1210, 2069, 2379, 2532
Mva1269I GAATGC 7 cut(s) 130, 858, 1129, 1363, 2145, 2252, 2504
MvaI CCWGG 5 cut(s) 245, 368, 1210, 2069, 2379
MvnI CGCG 1 cut(s) 1978
MwoI GCNNNNNNNGC 7 cut(s) 275, 821, 883, 998, 1534, 1652, 2149
NaeI GCCGGC 1 cut(s) 1188
NciI CCSGG 1 cut(s) 2532
NcoI CCATGG 1 cut(s) 2110
NdeI CATATG 3 cut(s) 145, 928, 2820
NgoMIV GCCGGC 1 cut(s) 1186
NlaIV GGNNCC 3 cut(s) 742, 1493, 2815
NmuCI GTSAC 3 cut(s) 1291, 1496, 1630
NsiI ATGCAT 3 cut(s) 1619, 2145, 2870
NspI RCATGY 3 cut(s) 1617, 2868, 2872
NspV TTCGAA 1 cut(s) 1121
PaeI GCATGC 2 cut(s) 2868, 2872
PaeR7I CTCGAG 2 cut(s) 557, 691
PagI TCATGA 1 cut(s) 2830
PaqCI CACCTGC 1 cut(s) 1064
PciSI GCTCTTC 3 cut(s) 249, 1083, 2088
PcsI WCGNNNNNNNCGW 1 cut(s) 1975
PctI GAATGC 7 cut(s) 130, 858, 1129, 1363, 2145, 2252, 2504
PdiI GCCGGC 1 cut(s) 1188
PdmI GAANNNNTTC 1 cut(s) 1117
PfeI GAWTC 8 cut(s) 335, 587, 605, 1016, 1049, 2522, 2648, 2783
PflMI CCANNNNNTGG 2 cut(s) 680, 1943
PfoI TCCNGGA 1 cut(s) 366
PkrI GCNGC 8 cut(s) 363, 498, 952, 1061, 1835, 2269, 2796, 2874
PleI GAGTC 1 cut(s) 2189
PpsI GAGTC 1 cut(s) 2189
PshAI GACNNNNGTC 1 cut(s) 1637
PshBI ATTAAT 2 cut(s) 2055, 2552
PsiI TTATAA 1 cut(s) 1875
Psp124BI GAGCTC 2 cut(s) 20, 221
Psp6I CCWGG 5 cut(s) 243, 366, 1208, 2067, 2377
PspEI GGTNACC 1 cut(s) 2852
PspGI CCWGG 5 cut(s) 243, 366, 1208, 2067, 2377
PspN4I GGNNCC 3 cut(s) 742, 1493, 2815
PspPI GGNCC 6 cut(s) 770, 880, 1346, 1492, 2528, 2778
PspXI VCTCGAGB 1 cut(s) 691
PstI CTGCAG 3 cut(s) 955, 1061, 2601
PstNI CAGNNNCTG 1 cut(s) 1545
PsuI RGATCY 3 cut(s) 829, 1141, 1472
PvuII CAGCTG 1 cut(s) 1328
RsaI GTAC 5 cut(s) 553, 1764, 1935, 2540, 2594
RsaNI GTAC 5 cut(s) 552, 1763, 1934, 2539, 2593
RseI CAYNNNNRTG 4 cut(s) 114, 2115, 2261, 2819
SacI GAGCTC 2 cut(s) 20, 221
SapI GCTCTTC 3 cut(s) 249, 1083, 2088
SaqAI TTAA 7 cut(s) 285, 650, 933, 1802, 1962, 2055, 2552
SatI GCNGC 8 cut(s) 362, 497, 951, 1060, 1834, 2268, 2795, 2873
Sau96I GGNCC 6 cut(s) 770, 880, 1346, 1492, 2528, 2778
SbfI CCTGCAGG 1 cut(s) 2601
SchI GAGTC 1 cut(s) 2189
ScrFI CCNGG 6 cut(s) 245, 368, 1210, 2069, 2379, 2532
SdaI CCTGCAGG 1 cut(s) 2601
SduI GDGCHC 7 cut(s) 20, 221, 977, 1530, 1800, 1896, 2816
SfaNI GCATC 3 cut(s) 256, 1751, 2152
SfcI CTRYAG 4 cut(s) 591, 951, 1057, 2597
Sfr274I CTCGAG 2 cut(s) 557, 691
SfuI TTCGAA 1 cut(s) 1121
SinI GGWCC 5 cut(s) 770, 880, 1346, 1492, 2528
SlaI CTCGAG 2 cut(s) 557, 691
SmiMI CAYNNNNRTG 4 cut(s) 114, 2115, 2261, 2819
SmlI CTYRAG 4 cut(s) 557, 691, 1350, 2807
SmoI CTYRAG 4 cut(s) 557, 691, 1350, 2807
SpeI ACTAGT 1 cut(s) 1627
SphI GCATGC 2 cut(s) 2868, 2872
Sse8387I CCTGCAGG 1 cut(s) 2601
SsiI CCGC 5 cut(s) 32, 57, 494, 544, 1415
SspMI CTAG 5 cut(s) 188, 1146, 1476, 1628, 2244
SstI GAGCTC 2 cut(s) 20, 221
StyD4I CCNGG 6 cut(s) 243, 366, 1208, 2067, 2377, 2530
StyI CCWWGG 1 cut(s) 2110
TaaI ACNGT 7 cut(s) 194, 1072, 1430, 1930, 2668, 2804, 2846
TaiI ACGT 6 cut(s) 535, 1641, 1972, 2119, 2756, 2894
TaqI TCGA 7 cut(s) 23, 558, 692, 1024, 1121, 1322, 2881
TatI WGTACW 1 cut(s) 551
TfiI GAWTC 8 cut(s) 335, 587, 605, 1016, 1049, 2522, 2648, 2783
Tru1I TTAA 7 cut(s) 285, 650, 933, 1802, 1962, 2055, 2552
Tru9I TTAA 7 cut(s) 285, 650, 933, 1802, 1962, 2055, 2552
TscAI CASTG 6 cut(s) 72, 199, 1573, 1744, 1933, 2205
TseFI GTSAC 3 cut(s) 1291, 1496, 1630
TseI GCWGC 8 cut(s) 361, 496, 950, 1059, 1833, 2267, 2794, 2872
Tsp45I GTSAC 3 cut(s) 1291, 1496, 1630
TspGWI ACGGA 5 cut(s) 680, 762, 827, 1484, 2163
TspRI CASTG 6 cut(s) 72, 199, 1573, 1744, 1933, 2205
Van91I CCANNNNNTGG 2 cut(s) 680, 1943
VpaK11BI GGWCC 5 cut(s) 770, 880, 1346, 1492, 2528
VspI ATTAAT 2 cut(s) 2055, 2552
XagI CCTNNNNNAGG 1 cut(s) 2600
XapI RAATTY 8 cut(s) 89, 210, 684, 760, 1156, 1261, 2365, 2638
XceI RCATGY 3 cut(s) 1617, 2868, 2872
XcmI CCANNNNNNNNNTGG 2 cut(s) 2385, 2758
XhoI CTCGAG 2 cut(s) 557, 691
XmnI GAANNNNTTC 1 cut(s) 1117
XspI CTAG 5 cut(s) 188, 1146, 1476, 1628, 2244
ZraI GACGTC 1 cut(s) 1639
Zsp2I ATGCAT 3 cut(s) 1619, 2145, 2870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.