MD14G1015300.v1.1

Permuted single zf-CXXC unit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002894
Physical Location & Seq
Forward (+)
1366933 .. 1377317
10385 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1015300.v1.1.491

Sequence Viewer

Length: 5427 bp
ATGGGTGAAGAGGGAGGAGATCGGAGTTCTTGCTGTTGGATGCCACCAACACCTTACAGGCCTATTGTCCCAAGACCGGAGCCCAAATTGCAGCCGGAGATTGCGACGGCCTCGAGACCCTTGGCCTTCGGGGTAGAAGAAACTCAAGAGAATAGAGTAGTTATAGACTTGGATCCGGATCCGGAGGAAGAGAGCGGGTTTGTTTACCATGGGGCTGGGGCCGGAGCCGGAGCCGGCGTTGAAAGTTCAACGGACGCTTATTTTTGCCGCGTGGCGCAGTGGAGAGACGTTCCTTGTAAAGAGCTCATGATCCTTGCGGTGGCCTCTGCAAATGACGGGAGTGGTGGTGCAGTGCTTGGAGATACTCAGCATGAATATTCGTTCCCTGACAACCATCCTTATGATCTGAATGTTCCACCTCCGACTACATATGGCCAATTTGCACCTATAACACCAGATAAGTCCATGAGAGTAGACAGTGTTCAAATGTCTCAAATTCCGAGCTCCAACGCAGACGATGGACGAGGCCAGGAGATAGAAGAGCAATGGGATGCTAATTCTGCGACAATTAACATAATTGATCTGGAAAACAATAAGGATATAGAAAAATCTGCCGTGGATTCATCACAAGCTACTTTCTCCACGCAGCTCCAGGAACATTGCAACCCTGACAAGGAGGTGAACATCATTATAGACTTGAACAATACACCACAACCAAAACAAAGAAGGAGAAAGCACAGGCCGAAGGTGATTATAGAAGGCAAGCCAAAAAGAAACCCACAGCCTTCTGGTTCTATGGAAAATCCAAAACCCAAACGTAAGTATGTGCGGAAGAGTACACTCGAGAAAAACAAAACTCCTCCACCACAGGAATCTACAGAGCATATTGATTCAAATAATCTGAAGCCTCCGAAGAGATCATGCAGAAGGACTTTAAACTATGCCGACACAGAAGAAACAATAGATGGAATTTCCTCAAGCAAGTCTTTCAATGTGGGTTCACAATCACAGGAGATGAATGTTGGCACCGATGGAGTTCAATCAAATTCAACTGGACCGTGCAGGAATGAAGTTGAATTGGTGGCAGATAACACCCAAGCAGGCATAGCCCAAGATCTCGTCCGTTCTACAAGCCGAATGCTGAAACATTACTTGTCATTGCCAGACCAGCAACCTCCAGTCACACCACAACAAATTAGAGGTTCTACTACATATGTTAATTCCCAAAAGGAGGCTGCAGAAGAAAAAGGACAAATGAGCACTTGTAGTGGACAGGCAAACACTGCACAAACCATGTTGGATTGTGACACTCGATCATCCCAACGAAGTGCAAATGATTCCACCTGCAGCACAACTACAGTTCTGGCAACAGAAGAAGAGCAAGCAAAAAGATCAGAGAGAAACTATTTGAATGCTGTTGAGCAAGCATATCCTAGAACTGGAAATTTGTTCGGGGCTAACTATAATAATTTGCCGGCATACTACAATGTAATGTCCTGGGTGCATTTTCCATACATATACAAGAAGAAGAGGACCGACAAGGCGCAAAATTCTACCATACCAAGCACCTCATATCGTGTGACTATGGCAGAAAATGTATGGAGACCATCGACAGCTGGCTACTTGACTTCTGGTCCTCAAGTGAATGCTGATAATTTTTCAACTGCACTTGGAGAAGCAGGAAAAACCCCTCAGGAGAGACCGCAAGGTGTTCACCGTTTCCTGCCTTTGTATCAGACAGAGAGGTCAACAAAGAGAAGATCTAGTTGCCCTACAAGGGTCCGTGACTTAGCTTCCCTAACCAGAACACCAGAGCACATTCTGCACAGAACCTGTCTCGTCAAACAACCACCCACTGATGGCAATGGTCAAAGAGTGAACCACTTTGACGGATCTCAGACATGCATTGACGCGCTAGTCACAGACGTCGGTGCAACACTCGCAAAAAAGAAGAGGACAAAGAGAAATCCACTTTCCAGTTCACAACGAGGTCTTGTAATATATCAGAACCAGCAATCTTTCGCCACTGCATCAGGTGTTCCTCCAGAAGTACCATTTGAACAACTGCTTTCTGCTATTACTGAGCACTTAAAATGTTTGAACATAAATAGAGAAAATAGCAGCAGATTTGCCTATCAAGGGTATAATGTGATCTCTTCTTATAAGGCACAATATCAAGAGCACAATGCACTTGTTCTTTATAGAAGAGATGGCACTGTTGTACCATTTGATGGTTCTTTTGATCCAATTAAGAAACGTAAAGCACGACCTAAAGTTGACCTTGATCAAGAGACTGATAGAGTATGGAAGCTTCTGCTGGAAAATATAAATAGTGAAGGTATTAATGGAACAGACCAGGAGAAAGAAAAATGGTGGGAAGAAGAGCGTAGAGTGTTCCATGGACGTGCAGACAATTTTATTGCACGAATGCATCTTGTGCAAGGTGATAGACGCTTCTCTCCGTGGAAGGGATCAGTTCTGGACTCAGTGGTTGGAGTTTTCCTTACACAAAATGTCTCAGACCACCTCTCTAGCTCTGCATTCATGTCAATGGCTGCACATTTTCCCCTGAAGTCAAGGAGCAACGAAAAATCATGTGATGAAGACGTTGCGAGCTTCGTAGTAGATGAACCAGAAGTGTGCCTTAGTGAGAATTCAAACCAGCCAGGTTGTGACTGGAGTTCCCTCACGTTCCATGATGCTGAGCATAGTGAAGAAAAAGTTGTCAATGGAAATGAGAACTCTGGAAGCACTACTGAAGGTATCTCAACAAATGAAGCAGAATGCAAGCTATCACATCCATCTGAATCTGGTCCGGGGTTGTACCCTAACTCATTAATGAACAAATCAACCACCAAGATCACAAGAACAGGATCGTACCTGCAGGAGGATATGAGAACTTATGATGGAGTTTCATCTCAAAATTCTGTGGATTCTTCAACTTCACAAACTGTGGAAAAGACAGGATCATGCGAGAGCAACTCAGAAACAGAGTATCCACCTAATAGATGTGAAAACAGCAGTTTAGATCACTCCACTTCGTTTGTGGAGCTTTTACAGAGGGCCGAATCAAGTATGCTGCATTACAGTCTCAAGGGGACCTATATGTCCTCTCATGACACATCAAACTGTGGAGGTTACCAACCTGAATGTATGCAGCATAATGATCAAAGATGTGAGATAAACAGAGAAGAAGCATCCCTAGACCCTTCCAGTAACTGTTGTTTGAATCTAACCCCCCACCCAGGAGTACGGCAAGTTGAGTGCTTTGATTTGTTCGCAGAAGTAACCGAGTCTTCTTATACATCCAAAAATAAATGGGAAGACAGTCTGAGTGAAAGAAGTGTACTAACAGCAGAATCTCCCAGTCAAGATACAATAAATAATAAGCTGACAGCAAATGTTCAAGAAACACCAAGATGTTCCAGAAATTCGTCCAATAACATTCAGGCAGGCAATAATATGGCCCAGTCTCAACTCGGGTGGGTTGGGAACTTGAATAATGTTGACATACATTCTCAGGAGCAAAACAATAAGATACAGCAAAGTCGCTTGAATGTTTCTGGAGGGACCACAGATGTTAGGCAGAAGGCAGCAGAGTTGGGTTTAAATGAGCAGAGTAATTCAGTAAGCAAGGAGTTCACTAGTACAAATGCTGCTACCTCCAAAACAAAGAACAGAAGAGCTGGAAAAGAGAACAAAGATCAACAAGATTGGGACAAATTAAGGAAACAAGCAGAATCAAATGGAAAGAAAAGGGAAAAGACAGAAAATACAATGGATTCCTTGGACTGGGAAGCCGTAAGATGTGCAGATGTTAATGAGATTGCCCAAACCATCAAAGAGCGGGGAATGAATAATATGCTTGCAGAACGAATAAAGGATTTCCTTAACCGACTGCACAGAGAACATGGTAGTGTTAATCTTGAATGGTTGAGGGACGTTCCACCTGACCAAGCAAAAGAGTATCTGCTGAGCTTTCGTGGATTGGGGCTGAAAAGTGTGGAGTGTGTGAGGCTTTTGACACTGCACCATCTTGCTTTTCCGGTGGACACAAATGTTGGACGTATAGCTGTACGGCTTGGATGGGTACCCCTTCAGCCACTGCCTGAGTCACTGCAGTTGCATCTTCTTGAATTGTACCCAGTGCTGGAGTCCATACAAAAGTATTTGTGGCCACGACTGTGCAAGCTTGACCAAAGAACATTGTACGAGCTGCATTACCAAATGATAACATTTGGAAAGGTCTTCTGCACTAAAAGCAAGCCGAATTGTAATGCATGCCCTCTGAGAGCAGAATGTAGGCATTTTGCGAGTGCATTTGCGAGTGCGAGGCTTGCCCTGCCGGGACCAGAGGAGCAAAGCATAGTGAGTGCAACTGAAGACAGAACTACCCATCCAAACCCTGCTGGGATCAACAATAGAATGCCCTTGCCCTTCCCTCAGGCAACATACCAGCAATTGGAAGCAAGTCAGAAATCAGAAGTAAAATCTACAGCTGGTCATTGTGAACCTACAACATACCATCAATTGGAAGTAAGCGAGATATCAGAAGTAAAAGCTGCAGTTGGTAACTGTGAACCTATTGTTGAAGAACCAGCATCACCAGAGCCAGTGTGTACACAGATATCAGAGGACATTGAGGACTTCTGTGATGATCCTGATGAAATTCCTACTATTAAACTTAACATTGAAGAGTTCACTCAGACTTTGCAAAATTATATGGAAAAAAACATGGAGCTTCAAGAAGGTGACATGTCAAAAGCTTTAGTCTCTCTGACACCAGAAGCTGCATCTTTACCAACTCCCAAGCTTAAGAACGTGAGCCGATTGCGAACTGAGCACCAAGTCTATGAACTTCCTGATACGCACCCTCTTCTAGAAATGTTGCATATGGATAAGCGAGAACCTGATGACCCGTGTAATTACCTTCTGGCTATTTGGACTCCAGGTGAAACTCCAAATTCCATTCAACCACCAGAGAAAAGGTGTGGTTCTCAAGAACTTGGCAAGTTGTGTGACGATAAGGAATGTTTCTCATGCAACAGTTCACGGGAAGCAAATTCACAAACAGTCCGAGGGACTCTCTTGATACCATGCCGAACAGCAATGAGAGGGAGCTTTCCACTTAACGGAACCTACTTTCAAGTTAACGAGGTATTTGCCGACCATGATTCGAGCATTAACCCAATTGATGTTCCAAGAGCTTGGTTGTGGAAGCTAAATAGGAGAACCGTGTACTTCGGAACCTCGATACCAACAATATTTAAAGGGTTATCGACACCAGAAATTCAGCAATGCTTCTGGAGAGGATTTGTCTGCGTGAGAGGATTTCACCAAAAGACTCGAGCACCGCGCCCATTACTGGCCCGACTGCACTTCCCAGCCAGCAAGTTGGCCAGAACAAAAGATAAGAGAGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003906 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016458 GO:0016787 GO:0016798 GO:0016799 GO:0019104 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031935 GO:0031936 GO:0032259 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035510 GO:0040029 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045815 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0080111 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1809

Amino Acids

202.43

Weight (kDa)

5.82

Isoelectric Point (pI)

54.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Perm-CXXC PF15629 1660 - 1691 3.1e-14 Permuted single zf-CXXC unit
RRM_DME PF15628 1694 - 1794 1.7e-52 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2163
AarI CACCTGC 1 cut(s) 1352
AasI GACNNNNNNGTC 3 cut(s) 1744, 1916, 3106
AatII GACGTC 1 cut(s) 1929
Acc36I ACCTGC 2 cut(s) 1352, 2889
Acc65I GGTACC 1 cut(s) 4090
AccB1I GGYRCC 2 cut(s) 1025, 4090
AccB7I CCANNNNNTGG 3 cut(s) 2231, 4462, 4531
AccBSI CCGCTC 2 cut(s) 195, 3847
AccI GTMKAC 1 cut(s) 474
AccII CGCG 3 cut(s) 270, 1913, 5356
AccIII TCCGGA 2 cut(s) 175, 181
AciI CCGC 7 cut(s) 195, 268, 317, 829, 1703, 3847, 5354
AcoI YGGCCR 3 cut(s) 433, 4175, 5397
AcuI CTGAAG 5 cut(s) 923, 2591, 2778, 4082, 4401
AcyI GRCGYC 1 cut(s) 1926
AflII CTTAAG 1 cut(s) 4814
AflIII ACRYGT 1 cut(s) 4755
AhdI GACNNNNNGTC 1 cut(s) 1632
AhlI ACTAGT 1 cut(s) 3644
AjiI CACGTC 1 cut(s) 2405
AjnI CCWGG 7 cut(s) 528, 651, 1496, 2355, 2665, 3244, 4948
AleI CACNNNNGTG 1 cut(s) 4183
AloI GAACNNNNNNTCC 2 cut(s) 4381, 4413
Alw21I GWGCWC 8 cut(s) 306, 506, 1262, 1818, 2088, 2184, 4845, 5353
AlwNI CAGNNNCTG 4 cut(s) 1833, 3219, 4105, 4790
Ama87I CYCGRG 4 cut(s) 112, 842, 3479, 5346
Aor13HI TCCGGA 2 cut(s) 175, 181
AseI ATTAAT 2 cut(s) 2343, 2837
Asp718I GGTACC 1 cut(s) 4090
AspLEI GCGC 4 cut(s) 277, 1546, 1915, 5358
AsuC2I CCSGG 2 cut(s) 2817, 4347
AsuHPI GGTGA 9 cut(s) 17, 691, 760, 1706, 2456, 4596, 4763, 4964, 5327
AvaI CYCGRG 4 cut(s) 112, 842, 3479, 5346
AvaII GGWCC 8 cut(s) 1055, 1533, 1634, 1780, 2813, 3099, 3570, 4349
AxyI CCTNAGG 2 cut(s) 1692, 4443
BaeI ACNNNNGTAYC 2 cut(s) 2205, 2238
BalI TGGCCA 3 cut(s) 435, 4177, 5399
BamHI GGATCC 2 cut(s) 172, 178
BanI GGYRCC 2 cut(s) 1025, 4090
BanII GRGCYC 3 cut(s) 84, 306, 506
BarI GAAGNNNNNNTAC 2 cut(s) 2295, 2327
BbsI GAAGAC 5 cut(s) 2610, 3288, 3330, 4240, 4389
Bbv12I GWGCWC 8 cut(s) 306, 506, 1262, 1818, 2088, 2184, 4845, 5353
BceAI ACGGC 5 cut(s) 123, 599, 3269, 3785, 4094
BcgI CGANNNNNNTGC 2 cut(s) 5144, 5178
BciT130I CCWGG 7 cut(s) 530, 653, 1498, 2357, 2667, 3246, 4950
BciVI GTATCC 1 cut(s) 3006
BclI TGATCA 2 cut(s) 2284, 3166
BcnI CCSGG 2 cut(s) 2817, 4347
BcuI ACTAGT 1 cut(s) 3644
BfaI CTAG 7 cut(s) 1434, 1764, 1916, 2532, 3203, 3645, 4880
BfmI CTRYAG 8 cut(s) 876, 1236, 1345, 1356, 2882, 4118, 4494, 4563
BfrI CTTAAG 1 cut(s) 4814
BfuAI ACCTGC 2 cut(s) 1352, 2889
BfuI GTATCC 1 cut(s) 3006
BglII AGATCT 2 cut(s) 1114, 1760
BlpI GCTNAGC 2 cut(s) 2703, 3974
Bme1390I CCNGG 9 cut(s) 530, 653, 1498, 2357, 2667, 2817, 3246, 4347, 4950
Bme18I GGWCC 8 cut(s) 1055, 1533, 1634, 1780, 2813, 3099, 3570, 4349
BmeRI GACNNNNNGTC 1 cut(s) 1632
BmeT110I CYCGRG 4 cut(s) 112, 842, 3479, 5346
BmgBI CACGTC 1 cut(s) 2405
BmrFI CCNGG 9 cut(s) 530, 653, 1498, 2357, 2667, 2817, 3246, 4347, 4950
BmrI ACTGGG 4 cut(s) 3360, 3463, 3802, 4139
BmsI GCATC 9 cut(s) 30, 541, 2039, 2440, 2689, 3206, 4135, 4610, 4802
BmuI ACTGGG 4 cut(s) 3360, 3463, 3802, 4139
BpiI GAAGAC 5 cut(s) 2610, 3288, 3330, 4240, 4389
BplI GAGNNNNNCTC 4 cut(s) 2966, 2998, 5070, 5102
BpmI CTGGAG 8 cut(s) 635, 1161, 2028, 2698, 3585, 4172, 4932, 5326
Bpu1102I GCTNAGC 2 cut(s) 2703, 3974
BpuEI CTTGAG 5 cut(s) 129, 961, 1623, 3077, 4983
BpuMI CCSGG 2 cut(s) 2817, 4347
BsaBI GATNNNNATC 1 cut(s) 177
BsaHI GRCGYC 1 cut(s) 1926
BsaI GGTCTC 3 cut(s) 109, 1597, 1693
BsaWI WCCGGW 4 cut(s) 76, 175, 181, 4045
BsaXI ACNNNNNCTCC 4 cut(s) 222, 252, 331, 361
Bse118I RCCGGY 2 cut(s) 233, 1474
Bse21I CCTNAGG 2 cut(s) 1692, 4443
Bse3DI GCAATG 6 cut(s) 551, 658, 1157, 1870, 5115, 5303
Bse8I GATNNNNATC 1 cut(s) 177
BseAI TCCGGA 2 cut(s) 175, 181
BseBI CCWGG 7 cut(s) 530, 653, 1498, 2357, 2667, 3246, 4950
BseGI GGATG 9 cut(s) 45, 394, 556, 1316, 2797, 3197, 3305, 4091, 4396
BseJI GATNNNNATC 1 cut(s) 177
BseMI GCAATG 6 cut(s) 551, 658, 1157, 1870, 5115, 5303
BseRI GAGGAG 3 cut(s) 30, 849, 4370
BseYI CCCAGC 3 cut(s) 215, 4409, 5383
Bsh1236I CGCG 3 cut(s) 270, 1913, 5356
BshNI GGYRCC 2 cut(s) 1025, 4090
BsiHKAI GWGCWC 8 cut(s) 306, 506, 1262, 1818, 2088, 2184, 4845, 5353
BsiHKCI CYCGRG 4 cut(s) 112, 842, 3479, 5346
BslFI GGGAC 7 cut(s) 53, 3112, 3583, 3731, 3953, 4362, 5095
BsmBI CGTCTC 1 cut(s) 279
BsmFI GGGAC 7 cut(s) 53, 3112, 3583, 3731, 3953, 4362, 5095
BsmI GAATGC 7 cut(s) 1143, 1417, 1651, 2433, 2540, 2789, 4431
Bso31I GGTCTC 3 cut(s) 109, 1597, 1693
BsoBI CYCGRG 4 cut(s) 112, 842, 3479, 5346
Bsp1286I GDGCHC 9 cut(s) 84, 306, 506, 1262, 1818, 2088, 2184, 4845, 5353
Bsp13I TCCGGA 2 cut(s) 175, 181
Bsp1407I TGTACA 1 cut(s) 4619
Bsp1720I GCTNAGC 2 cut(s) 2703, 3974
Bsp19I CCATGG 2 cut(s) 208, 2398
BspACI CCGC 7 cut(s) 195, 268, 317, 829, 1703, 3847, 5354
BspEI TCCGGA 2 cut(s) 175, 181
BspFNI CGCG 3 cut(s) 270, 1913, 5356
BspHI TCATGA 2 cut(s) 306, 3115
BspMAI CTGCAG 5 cut(s) 1240, 1349, 2886, 4122, 4567
BspMI ACCTGC 2 cut(s) 1352, 2889
BspQI GCTCTTC 4 cut(s) 534, 1371, 2376, 3676
BspT107I GGYRCC 2 cut(s) 1025, 4090
BspTI CTTAAG 1 cut(s) 4814
BspTNI GGTCTC 3 cut(s) 109, 1597, 1693
BsrBI CCGCTC 2 cut(s) 195, 3847
BsrDI GCAATG 6 cut(s) 551, 658, 1157, 1870, 5115, 5303
BsrFI RCCGGY 2 cut(s) 233, 1474
BsrGI TGTACA 1 cut(s) 4619
BssAI RCCGGY 2 cut(s) 233, 1474
BssNI GRCGYC 1 cut(s) 1926
BssT1I CCWWGG 4 cut(s) 120, 208, 2398, 3786
Bst2UI CCWGG 7 cut(s) 530, 653, 1498, 2357, 2667, 3246, 4950
BstACI GRCGYC 1 cut(s) 1926
BstAFI CTTAAG 1 cut(s) 4814
BstAPI GCANNNNNTGC 3 cut(s) 1283, 1822, 2437
BstAUI TGTACA 1 cut(s) 4619
BstDSI CCRYGG 4 cut(s) 208, 615, 2398, 2462
BstEII GGTNACC 1 cut(s) 3137
BstENI CCTNNNNNAGG 1 cut(s) 2885
BstF5I GGATG 9 cut(s) 45, 394, 556, 1316, 2797, 3197, 3305, 4091, 4396
BstFNI CGCG 3 cut(s) 270, 1913, 5356
BstHHI GCGC 4 cut(s) 277, 1546, 1915, 5358
BstNI CCWGG 7 cut(s) 530, 653, 1498, 2357, 2667, 3246, 4950
BstNSI RCATGY 3 cut(s) 1905, 4284, 4759
BstPI GGTNACC 1 cut(s) 3137
BstSCI CCNGG 9 cut(s) 528, 651, 1496, 2355, 2665, 2815, 3244, 4345, 4948
BstSFI CTRYAG 8 cut(s) 876, 1236, 1345, 1356, 2882, 4118, 4494, 4563
BstUI CGCG 3 cut(s) 270, 1913, 5356
BstV2I GAAGAC 5 cut(s) 2610, 3288, 3330, 4240, 4389
BstX2I RGATCY 5 cut(s) 172, 178, 1114, 1760, 1892
BstXI CCANNNNNNTGG 3 cut(s) 215, 5208, 5395
BstYI RGATCY 5 cut(s) 172, 178, 1114, 1760, 1892
Bsu36I CCTNAGG 2 cut(s) 1692, 4443
BsuI GTATCC 1 cut(s) 3006
BtgI CCRYGG 4 cut(s) 208, 615, 2398, 2462
BtrI CACGTC 1 cut(s) 2405
BtsCI GGATG 9 cut(s) 45, 394, 556, 1316, 2797, 3197, 3305, 4091, 4396
BtsI GCAGTG 7 cut(s) 284, 357, 1281, 2025, 4025, 4103, 4115
BveI ACCTGC 2 cut(s) 1352, 2889
CaiI CAGNNNCTG 4 cut(s) 1833, 3219, 4105, 4790
CciI TCATGA 2 cut(s) 306, 3115
CfoI GCGC 4 cut(s) 277, 1546, 1915, 5358
Cfr10I RCCGGY 2 cut(s) 233, 1474
CseI GACGC 3 cut(s) 263, 1919, 2460
CspCI CAANNNNNGTGG 4 cut(s) 3025, 3060, 3464, 3499
DraI TTTAAA 3 cut(s) 936, 3609, 5269
DrdI GACNNNNNNGTC 3 cut(s) 1744, 1916, 3106
DriI GACNNNNNGTC 1 cut(s) 1632
DseDI GACNNNNNNGTC 3 cut(s) 1744, 1916, 3106
EaeI YGGCCR 3 cut(s) 433, 4175, 5397
Eam1105I GACNNNNNGTC 1 cut(s) 1632
Ecl136II GAGCTC 2 cut(s) 304, 504
Eco130I CCWWGG 4 cut(s) 120, 208, 2398, 3786
Eco147I AGGCCT 1 cut(s) 61
Eco24I GRGCYC 3 cut(s) 84, 306, 506
Eco31I GGTCTC 3 cut(s) 109, 1597, 1693
Eco32I GATATC 2 cut(s) 4548, 4629
Eco47I GGWCC 8 cut(s) 1055, 1533, 1634, 1780, 2813, 3099, 3570, 4349
Eco53kI GAGCTC 2 cut(s) 304, 504
Eco57I CTGAAG 5 cut(s) 923, 2591, 2778, 4082, 4401
Eco81I CCTNAGG 2 cut(s) 1692, 4443
Eco88I CYCGRG 4 cut(s) 112, 842, 3479, 5346
Eco91I GGTNACC 1 cut(s) 3137
EcoICRI GAGCTC 2 cut(s) 304, 504
EcoNI CCTNNNNNAGG 1 cut(s) 2885
EcoO109I RGGNCCY 1 cut(s) 3099
EcoO65I GGTNACC 1 cut(s) 3137
EcoRI GAATTC 1 cut(s) 2653
EcoRII CCWGG 7 cut(s) 528, 651, 1496, 2355, 2665, 3244, 4948
EcoRV GATATC 2 cut(s) 4548, 4629
EcoT14I CCWWGG 4 cut(s) 120, 208, 2398, 3786
EcoT22I ATGCAT 3 cut(s) 1907, 2433, 4282
EcoT38I GRGCYC 3 cut(s) 84, 306, 506
ErhI CCWWGG 4 cut(s) 120, 208, 2398, 3786
Esp3I CGTCTC 1 cut(s) 279
FalI AAGNNNNNCTT 6 cut(s) 970, 1002, 2265, 2297, 2628, 2660
FaqI GGGAC 7 cut(s) 53, 3112, 3583, 3731, 3953, 4362, 5095
FauI CCCGC 2 cut(s) 188, 3840
FauNDI CATATG 3 cut(s) 430, 1213, 4893
FbaI TGATCA 2 cut(s) 2284, 3166
FblI GTMKAC 1 cut(s) 474
FokI GGATG 9 cut(s) 52, 381, 563, 1303, 2784, 3184, 3292, 4098, 4383
FriOI GRGCYC 3 cut(s) 84, 306, 506
FspBI CTAG 7 cut(s) 1434, 1764, 1916, 2532, 3203, 3645, 4880
GlaI GCGC 4 cut(s) 276, 1545, 1914, 5357
GsaI CCCAGC 3 cut(s) 219, 4413, 5387
GsuI CTGGAG 8 cut(s) 635, 1161, 2028, 2698, 3585, 4172, 4932, 5326
HgaI GACGC 3 cut(s) 263, 1919, 2460
HhaI GCGC 4 cut(s) 277, 1546, 1915, 5358
Hin1I GRCGYC 1 cut(s) 1926
Hin6I GCGC 4 cut(s) 275, 1544, 1913, 5356
HinP1I GCGC 4 cut(s) 275, 1544, 1913, 5356
HincII GTYRAC 4 cut(s) 1749, 2278, 3508, 5152
HindII GTYRAC 4 cut(s) 1749, 2278, 3508, 5152
HindIII AAGCTT 4 cut(s) 2309, 4190, 4764, 4811
HpaI GTTAAC 1 cut(s) 5152
HphI GGTGA 9 cut(s) 17, 691, 760, 1706, 2456, 4596, 4763, 4964, 5327
Hpy99I CGWCG 2 cut(s) 109, 1931
Hsp92I GRCGYC 1 cut(s) 1926
HspAI GCGC 4 cut(s) 275, 1544, 1913, 5356
Kpn2I TCCGGA 2 cut(s) 175, 181
KpnI GGTACC 1 cut(s) 4094
KroI GCCGGC 2 cut(s) 233, 1474
KroNI GCCGGC 2 cut(s) 235, 1476
Ksp22I TGATCA 2 cut(s) 2284, 3166
KspAI GTTAAC 1 cut(s) 5152
LguI GCTCTTC 4 cut(s) 534, 1371, 2376, 3676
LweI GCATC 9 cut(s) 30, 541, 2039, 2440, 2689, 3206, 4135, 4610, 4802
MaeI CTAG 7 cut(s) 1434, 1764, 1916, 2532, 3203, 3645, 4880
MbiI CCGCTC 2 cut(s) 195, 3847
MfeI CAATTG 3 cut(s) 4460, 4529, 5190
MflI RGATCY 5 cut(s) 172, 178, 1114, 1760, 1892
MhlI GDGCHC 9 cut(s) 84, 306, 506, 1262, 1818, 2088, 2184, 4845, 5353
MlsI TGGCCA 3 cut(s) 435, 4177, 5399
MluNI TGGCCA 3 cut(s) 435, 4177, 5399
MlyI GAGTC 7 cut(s) 2477, 3302, 4121, 4163, 4939, 5077, 5338
MmeI TCCRAC 6 cut(s) 17, 446, 531, 1278, 2473, 4042
Mox20I TGGCCA 3 cut(s) 435, 4177, 5399
Mph1103I ATGCAT 3 cut(s) 1907, 2433, 4282
MroI TCCGGA 2 cut(s) 175, 181
MroNI GCCGGC 2 cut(s) 233, 1474
MscI TGGCCA 3 cut(s) 435, 4177, 5399
MslI CAYNNNNRTG 6 cut(s) 399, 2403, 2549, 3418, 3915, 4183
Msp20I TGGCCA 3 cut(s) 435, 4177, 5399
MspA1I CMGCKG 2 cut(s) 1616, 4499
MspCI CTTAAG 1 cut(s) 4814
MspR9I CCNGG 9 cut(s) 530, 653, 1498, 2357, 2667, 2817, 3246, 4347, 4950
MunI CAATTG 3 cut(s) 4460, 4529, 5190
Mva1269I GAATGC 7 cut(s) 1143, 1417, 1651, 2433, 2540, 2789, 4431
MvaI CCWGG 7 cut(s) 530, 653, 1498, 2357, 2667, 3246, 4950
MvnI CGCG 3 cut(s) 270, 1913, 5356
NaeI GCCGGC 2 cut(s) 235, 1476
NciI CCSGG 2 cut(s) 2817, 4347
NcoI CCATGG 2 cut(s) 208, 2398
NdeI CATATG 3 cut(s) 430, 1213, 4893
NgoMIV GCCGGC 2 cut(s) 233, 1474
NmuCI GTSAC 9 cut(s) 1180, 1304, 1579, 1784, 1918, 2672, 4113, 4751, 5018
NsiI ATGCAT 3 cut(s) 1907, 2433, 4282
NspI RCATGY 3 cut(s) 1905, 4284, 4759
OliI CACNNNNGTG 1 cut(s) 4183
PaeI GCATGC 1 cut(s) 4284
PaeR7I CTCGAG 3 cut(s) 112, 842, 5346
PagI TCATGA 2 cut(s) 306, 3115
PaqCI CACCTGC 1 cut(s) 1352
PceI AGGCCT 1 cut(s) 61
PciI ACATGT 1 cut(s) 4755
PciSI GCTCTTC 4 cut(s) 534, 1371, 2376, 3676
PcsI WCGNNNNNNNCGW 1 cut(s) 2263
PctI GAATGC 7 cut(s) 1143, 1417, 1651, 2433, 2540, 2789, 4431
PdiI GCCGGC 2 cut(s) 235, 1476
PflMI CCANNNNNTGG 3 cut(s) 2231, 4462, 4531
PfoI TCCNGGA 1 cut(s) 651
PleI GAGTC 7 cut(s) 2477, 3301, 4120, 4162, 4939, 5077, 5338
PpsI GAGTC 7 cut(s) 2477, 3301, 4120, 4162, 4939, 5077, 5338
PpuMI RGGWCCY 1 cut(s) 3099
PscI ACATGT 1 cut(s) 4755
PshBI ATTAAT 2 cut(s) 2343, 2837
PsiI TTATAA 1 cut(s) 2163
Psp124BI GAGCTC 2 cut(s) 306, 506
Psp5II RGGWCCY 1 cut(s) 3099
Psp6I CCWGG 7 cut(s) 528, 651, 1496, 2355, 2665, 3244, 4948
PspEI GGTNACC 1 cut(s) 3137
PspFI CCCAGC 3 cut(s) 215, 4409, 5383
PspGI CCWGG 7 cut(s) 528, 651, 1496, 2355, 2665, 3244, 4948
PspPPI RGGWCCY 1 cut(s) 3099
PspXI VCTCGAGB 1 cut(s) 5346
PsrI GAACNNNNNNTAC 2 cut(s) 465, 497
PstI CTGCAG 5 cut(s) 1240, 1349, 2886, 4122, 4567
PstNI CAGNNNCTG 4 cut(s) 1833, 3219, 4105, 4790
PsuI RGATCY 5 cut(s) 172, 178, 1114, 1760, 1892
PvuII CAGCTG 2 cut(s) 1616, 4499
RseI CAYNNNNRTG 6 cut(s) 399, 2403, 2549, 3418, 3915, 4183
SacI GAGCTC 2 cut(s) 306, 506
SapI GCTCTTC 4 cut(s) 534, 1371, 2376, 3676
SbfI CCTGCAGG 1 cut(s) 2886
SchI GAGTC 7 cut(s) 2477, 3302, 4121, 4163, 4939, 5077, 5338
ScrFI CCNGG 9 cut(s) 530, 653, 1498, 2357, 2667, 2817, 3246, 4347, 4950
SdaI CCTGCAGG 1 cut(s) 2886
SduI GDGCHC 9 cut(s) 84, 306, 506, 1262, 1818, 2088, 2184, 4845, 5353
SfaNI GCATC 9 cut(s) 30, 541, 2039, 2440, 2689, 3206, 4135, 4610, 4802
SfcI CTRYAG 8 cut(s) 876, 1236, 1345, 1356, 2882, 4118, 4494, 4563
Sfr274I CTCGAG 3 cut(s) 112, 842, 5346
SinI GGWCC 8 cut(s) 1055, 1533, 1634, 1780, 2813, 3099, 3570, 4349
SlaI CTCGAG 3 cut(s) 112, 842, 5346
SmiMI CAYNNNNRTG 6 cut(s) 399, 2403, 2549, 3418, 3915, 4183
SmlI CTYRAG 9 cut(s) 112, 144, 842, 976, 1638, 3092, 4814, 4998, 5346
SmoI CTYRAG 9 cut(s) 112, 144, 842, 976, 1638, 3092, 4814, 4998, 5346
SpeI ACTAGT 1 cut(s) 3644
SphI GCATGC 1 cut(s) 4284
Sse8387I CCTGCAGG 1 cut(s) 2886
SseBI AGGCCT 1 cut(s) 61
SsiI CCGC 7 cut(s) 195, 268, 317, 829, 1703, 3847, 5354
SspI AATATT 2 cut(s) 377, 5265
SspMI CTAG 7 cut(s) 1434, 1764, 1916, 2532, 3203, 3645, 4880
SstI GAGCTC 2 cut(s) 306, 506
StuI AGGCCT 1 cut(s) 61
StyD4I CCNGG 9 cut(s) 528, 651, 1496, 2355, 2665, 2815, 3244, 4345, 4948
StyI CCWWGG 4 cut(s) 120, 208, 2398, 3786
TaqI TCGA 8 cut(s) 113, 843, 1312, 1610, 5177, 5252, 5279, 5347
TaqII GACCGA 1 cut(s) 1550
TatI WGTACW 5 cut(s) 836, 3346, 3647, 4619, 5238
TauI GCSGC 1 cut(s) 270
TseFI GTSAC 9 cut(s) 1180, 1304, 1579, 1784, 1918, 2672, 4113, 4751, 5018
Tsp45I GTSAC 9 cut(s) 1180, 1304, 1579, 1784, 1918, 2672, 4113, 4751, 5018
TspGWI ACGGA 6 cut(s) 266, 1112, 1772, 1905, 2451, 5148
Van91I CCANNNNNTGG 3 cut(s) 2231, 4462, 4531
Vha464I CTTAAG 1 cut(s) 4814
VpaK11BI GGWCC 8 cut(s) 1055, 1533, 1634, 1780, 2813, 3099, 3570, 4349
VspI ATTAAT 2 cut(s) 2343, 2837
XagI CCTNNNNNAGG 1 cut(s) 2885
XbaI TCTAGA 1 cut(s) 4879
XceI RCATGY 3 cut(s) 1905, 4284, 4759
XcmI CCANNNNNNNNNTGG 2 cut(s) 2673, 3043
XhoI CTCGAG 3 cut(s) 112, 842, 5346
XmiI GTMKAC 1 cut(s) 474
XspI CTAG 7 cut(s) 1434, 1764, 1916, 2532, 3203, 3645, 4880
ZraI GACGTC 1 cut(s) 1927
Zsp2I ATGCAT 3 cut(s) 1907, 2433, 4282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.