Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006941

Physical Location & Seq

Genomic Coordinates Forward (+)

4854167 .. 4862753

Gene Structure 8587 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1043100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2898 bp

ATGACCCGACGAATCTCCGAGAAGCTCCGACCCTCTCTCTTCCCCTACAAGCCGACCCTCCGCTTTCTCTGTCCTCCTCCCCACCTCTCTCTCTCCTCCACGCTCCACCTCTGCAAGTCCCTCGCACGCGCCCTTGCCGCCAAAACCCTAGCCCTCTTCTCCCTCCCCTTTTCCGACCACGACCGCGACGCTATGGCCGATTCCTTCGGCGACGACTCCTTCTGGGACCTCGACTTCCGCCCCCATTTCCGCTCCCAGCGCCCCCGCCTCCACGACGACTACGATGACAACGGCGACAACGAAGACGTTTTGGGCAATGCCGTTGATAAGGTCTACCTCGTTCCTTACAGGTGGTGGAAGGAGGCTCGGACGGAGGAGGATCGCAATGGAGGGGTCCTTTACACTGCTACGACGGACGACGACGACGCTGGCGCTGAAATTGTATTGGATTTAAAGAAGAACGACGTCGTTGAAGAAGGGTTTTCGGGGCGCGAATACGCCTTGGTCCCTGAATCAACGTGGTGTCGCGCTCTCATACGGCATAATGATTTCAACGCAGCAGCGAAGGATAACGTGAGCTTTTTTGGTGCAGAAAACTTAAACGATATTTATTCTCTACAAATTAGGCTATTTGTTTCCTGGGAAACAAATTCATTGTTTGTAAAGATCACCCAAAAGGATAATGCAATAGTTTCCTACAAGAGGGCCTGTAATGTTTTCAGTTCAGAGTATGTTCCGTTGCATATTTGGGACTTCTCTGGGCAGACAACCCAATTTTTCATGAATGACAGAGTTAATTTGCCAAGCGATATTCCTGGACAACTGAACAAAGAGATTCTCCTTGAATTGCGACTCCATGGATTTTCAGACTGTATGAAAGGTTCAGATTGGAGGAGTGATGAAGTGGCTGATAAAGAGTCAAGTTTGGATGGTTCTACTTATGGTGGCTCTATTAAGATGAATGGAAGCACTGACTATGTGAATCCTTATTTAACTTCAACCAACTCCTTGCAAAGTGGTATAATCTATAGAGGAGTTGGTTCCTTAGGCCTTACTGGACTAGAGAATCTTGGGAATACTTGTTTTATGAACAGTGCCATCCAATGCTTGGTGCATACTCCAAAGCTGGTTGATTACTTTCTAGGAGATTACCGGAAGGACATAAATTACGCAAATCCACTAGGATTGAAAGGAGAGCTTGCTTTATCATTTGGAGAGTTGTTGAGGAGGTTATGGGCTCCAGGTGCAAGGTCAGTGACTCCAACGATGTTCAAGTCAAAACTTGCTAATTTTGCTCCCCAATTCAGTGGCTATAATCAGCATGATTCCCAGGAACTTCTTGCTTTTTTGTTGGATGGTCTCCACGAAGATCTAAATCGTGTAAAATGCAAACCTTACATTGAAGCTAAGGATGTGGAGGGTCGTCCAGATGATGAAGTAGCAGAAGAATATTGGCAAAATCACCTTGCTCGGAATGACTCAATCATTGTCGATGTGTGCCAAGGTCAGTACCGGTCAAAATTGGTATGCCCTGTTTGCAACAAGGTGTCTGTTATGTTTGACCCATTTATGTACCTTTCGCTACCGTTACCTTCAACATCAATGCGGACTATGACTTTGACGGTCCTAAGTACAGATGGGACTGCAATGCCATCTACATGTACAGTGACAGTGCCCCAGGGTGGGAGGTTGATGGATCTTACTAATGCCTTAAGCACTGCATGTTGCTTAAGAGATGATGAAACTCTGATGGTAGCTGAGGGGGCCTCTTTATGCCTTTTGCATTCTCTCCAATATACAAGAGTTGTATTTTTTCGTCTACTGGAAGATCCATTTGATTCATTAGCCTTGATCAGAGATGGTGACCTGTTGGTTGCTTATCGGTTACCAAAGGATTGTGAGTCATCCCTTTTGGTTACGTTTATTCATCAGCATATGGACGTGGGCTATAATAATGGAGAGATGGGATTAGTCCCACCGGCGTTTGGCGTACCCCTCATAGCAAGGTTGCCTGATGTTTGCAATGGATCTGATATTCGTAACAAGTTCCTGAAAGTACTAAACCCATACTTGATGCCTACTGGAGATGCATTAGATATTTTTGATGATGATGATATTGGAAATTATGCTACTGAAGATTCTAACAAGGAGGACGCTACTCCTTCTACGATTATGGATAATGATGCTTACGCGGACAGGGGATCTGGGGATGAAGCACATTTGGATAATGACTTCCAGTTTTACTTTACAGATACAAAGGGAGCTATAAGAGACACCTTGATAGACATGAATGAGCCAATAGTGGTCTCAAAGTTGCCTAAGAGGTTGAGTGTACTTGTTTTATGGTCTGATAAAATGACTAAAAAGTACGATACATGCCTTCTAAGCAATTTGCCGGAAATTTTCAAGCCCCAATTTTCTGTGAGGAGGCCTCAAGAATCTGTTTCTCTGTACAAATGCCTTGAAGCCTTTATGAAGGAAGAACCTCTAGGGCCAGAAGATATGTGGTACTGCCCTACCTGCAAGAAGCCTCAACAAGCTAGCAAAAAGTTAGATCTTTGGAGATTGCCTGAGATTTTGGTTATTCATCTGAAAAGATTCTCGTACAGCCATTATTTTAAGAACAAGCTAGAAACATTTGTTGATTTTCCAATTCATGATCTGGATTTTTCACGCTACATTGCCCACCAAATTAGCCAGTTATCCAGCCGATACATGTTGTATGCGATTAGTAATCACTATGGTGGCATGGGCGGTGGTCACTATACTGCATTTGCTCAACTCGGTAATGGTTCATGGTATGAGTTTGATGATGACAGGGTTTGTCATGTTGGGGAAGAGACGATCAAGACATCAGCTGCATATGTTCTTTTCTACCGAAGAGTGCCAGATGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

UCH

Protein Analysis

966

Amino Acids

109.24

Weight (kDa)

5.1

Isoelectric Point (pI)

39.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubiquitin_UBP8	PF25242	208 - 262	3.4e-28	UBP8-like, ubiquitin-like domain
UCH	PF00443	352 - 959	3.4e-73	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006941)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g20680 FvH4_6g20680 FvH4_6g20680
malus_domestica	MD00G1072700.v1.1 MD12G1043100.v1.1 MD14G1042200.v1.1
prunus_persica	Prupe.7G091700_v2.0.a1 Prupe.7G091700_v2.0.a1 Prupe.7G091700_v2.0.a1 Prupe.7G091700_v2.0.a1
pyrus_communis	pycom12g03720 pycom14g03580
rosa_chinensis	RchiOBHm_Chr3g0476581
rosa_laevigata	RLG00000001984 RLG00000023761
rosa_multiflora	Rmu_sc0008241.1_g000007 Rmu_sc0020945.1_g000001
rosa_roxburghii	Rroxscaffold_6G00405190
rosa_rugosa	Rorug03G0155400
rosa_samantha	Rh3AG206000 Rh3BG238600 Rh3CG232300 Rh3DG232200 Rh7DG310400
rosa_wichuraiana	Rw3G018760 Rw7G026340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	468
Acc36I	ACCTGC	1 cut(s)	2528
AccB7I	CCANNNNNTGG	1 cut(s)	1108
AccBSI	CCGCTC	1 cut(s)	252
AccI	GTMKAC	2 cut(s)	333, 1819
AccII	CGCG	5 cut(s)	129, 186, 492, 528, 2192
AciI	CCGC	9 cut(s)	61, 138, 184, 238, 250, 265, 1606, 2192, 2754
AclWI	GGATC	5 cut(s)	387, 1704, 1823, 2035, 2209
AcoI	YGGCCR	1 cut(s)	195
AcsI	RAATTY	2 cut(s)	649, 2400
AcuI	CTGAAG	1 cut(s)	2154
AcyI	GRCGYC	1 cut(s)	465
AfiI	CCNNNNNNNGG	5 cut(s)	702, 1108, 1682, 1683, 1985
AflII	CTTAAG	2 cut(s)	1711, 1729
AflIII	ACRYGT	2 cut(s)	1658, 2715
AgeI	ACCGGT	1 cut(s)	1512
AjiI	CACGTC	1 cut(s)	1942
AjnI	CCWGG	5 cut(s)	638, 814, 1240, 1329, 1677
AleI	CACNNNNGTG	1 cut(s)	2742
Alw26I	GTCTC	4 cut(s)	1364, 2265, 2311, 2834
AlwI	GGATC	5 cut(s)	387, 1704, 1823, 2035, 2209
AoxI	GGCC	6 cut(s)	195, 705, 1048, 1764, 2429, 2492
ApeKI	GCWGC	3 cut(s)	557, 560, 2858
ApoI	RAATTY	2 cut(s)	649, 2400
ArsI	GACNNNNNNTTYG	2 cut(s)	1600, 1632
AsiGI	ACCGGT	1 cut(s)	1512
Asp700I	GAANNNNTTC	1 cut(s)	2597
AspLEI	GCGC	5 cut(s)	131, 261, 434, 492, 530
AspS9I	GGNCC	7 cut(s)	226, 394, 505, 705, 1624, 1764, 2492
AsuHPI	GGTGA	3 cut(s)	661, 1454, 1874
AsuNHI	GCTAGC	1 cut(s)	2540
AvaII	GGWCC	4 cut(s)	226, 394, 505, 1624
AxyI	CCTNAGG	1 cut(s)	1045
BaeGI	GKGCMC	1 cut(s)	1677
BanII	GRGCYC	1 cut(s)	1240
BbsI	GAAGAC	1 cut(s)	309
BbvCI	CCTCAGC	1 cut(s)	1758
BbvI	GCAGC	3 cut(s)	569, 572, 2845
BccI	CCATC	9 cut(s)	923, 1106, 1349, 1631, 1660, 1687, 1744, 1853, 1957
BceAI	ACGGC	3 cut(s)	305, 307, 554
BcgI	CGANNNNNNTGC	2 cut(s)	103, 137
BciT130I	CCWGG	5 cut(s)	640, 816, 1242, 1331, 1679
BclI	TGATCA	1 cut(s)	1851
BcoDI	GTCTC	4 cut(s)	1364, 2265, 2311, 2834
BfaI	CTAG	7 cut(s)	149, 1061, 1142, 1181, 2489, 2541, 2630
BfmI	CTRYAG	1 cut(s)	1027
BfoI	RGCGCY	2 cut(s)	262, 435
BfrI	CTTAAG	2 cut(s)	1711, 1729
BfuAI	ACCTGC	1 cut(s)	2528
BglII	AGATCT	2 cut(s)	1369, 2554
BisI	GCNGC	4 cut(s)	138, 558, 561, 2859
BlsI	GCNGC	4 cut(s)	139, 559, 562, 2860
BmcAI	AGTACT	1 cut(s)	2058
Bme1390I	CCNGG	5 cut(s)	640, 816, 1242, 1331, 1679
Bme18I	GGWCC	4 cut(s)	226, 394, 505, 1624
BmgBI	CACGTC	1 cut(s)	1942
BmgT120I	GGNCC	7 cut(s)	226, 394, 505, 705, 1624, 1764, 2492
BmiI	GGNNCC	6 cut(s)	227, 395, 507, 1042, 1239, 1765
BmrFI	CCNGG	5 cut(s)	640, 816, 1242, 1331, 1679
BmsI	GCATC	3 cut(s)	2064, 2077, 2173
BmtI	GCTAGC	1 cut(s)	2544
BpiI	GAAGAC	1 cut(s)	309
BplI	GAGNNNNNCTC	4 cut(s)	1751, 1783, 2416, 2448
BpmI	CTGGAG	2 cut(s)	1224, 2103
Bpu10I	CCTNAGC	2 cut(s)	1407, 1758
BpuEI	CTTGAG	1 cut(s)	2418
BsaBI	GATNNNNATC	1 cut(s)	1374
BsaHI	GRCGYC	1 cut(s)	465
BsaI	GGTCTC	2 cut(s)	1364, 2311
BsaJI	CCNNGG	7 cut(s)	501, 639, 856, 1329, 1501, 1677, 1678
BsaWI	WCCGGW	2 cut(s)	1152, 1512
Bsc4I	CCNNNNNNNGG	5 cut(s)	702, 1108, 1682, 1683, 1985
Bse118I	RCCGGY	2 cut(s)	1512, 1978
Bse1I	ACTGG	5 cut(s)	1060, 1827, 2086, 2236, 2698
Bse21I	CCTNAGG	1 cut(s)	1045
Bse3DI	GCAATG	5 cut(s)	322, 391, 1653, 2029, 2679
Bse8I	GATNNNNATC	1 cut(s)	1374
BseBI	CCWGG	5 cut(s)	640, 816, 1242, 1331, 1679
BseDI	CCNNGG	7 cut(s)	501, 639, 856, 1329, 1501, 1677, 1678
BseGI	GGATG	6 cut(s)	934, 1098, 1360, 1417, 1904, 2215
BseJI	GATNNNNATC	1 cut(s)	1374
BseLI	CCNNNNNNNGG	5 cut(s)	702, 1108, 1682, 1683, 1985
BseMI	GCAATG	5 cut(s)	322, 391, 1653, 2029, 2679
BseMII	CTCAG	2 cut(s)	1749, 2562
BseNI	ACTGG	5 cut(s)	1060, 1827, 2086, 2236, 2698
BseRI	GAGGAG	7 cut(s)	66, 85, 389, 907, 1047, 1240, 2440
BseSI	GKGCMC	1 cut(s)	1677
BseXI	GCAGC	3 cut(s)	569, 572, 2845
BseYI	CCCAGC	1 cut(s)	255
BsgI	GTGCAG	1 cut(s)	609
Bsh1236I	CGCG	5 cut(s)	129, 186, 492, 528, 2192
Bsh1285I	CGRYCG	1 cut(s)	184
BshFI	GGCC	6 cut(s)	197, 707, 1050, 1766, 2431, 2494
BshTI	ACCGGT	1 cut(s)	1512
BsiEI	CGRYCG	1 cut(s)	184
BsiSI	CCGG	4 cut(s)	1153, 1513, 1979, 2396
BslFI	GGGAC	6 cut(s)	103, 239, 491, 764, 1654, 1958
BslI	CCNNNNNNNGG	5 cut(s)	702, 1108, 1682, 1683, 1985
BsmAI	GTCTC	4 cut(s)	1364, 2265, 2311, 2834
BsmBI	CGTCTC	1 cut(s)	2834
BsmFI	GGGAC	6 cut(s)	103, 239, 491, 764, 1654, 1958
BsmI	GAATGC	1 cut(s)	1783
BsnI	GGCC	6 cut(s)	197, 707, 1050, 1766, 2431, 2494
Bso31I	GGTCTC	2 cut(s)	1364, 2311
Bsp1286I	GDGCHC	2 cut(s)	1240, 1677
Bsp1407I	TGTACA	2 cut(s)	1661, 2451
Bsp19I	CCATGG	1 cut(s)	856
BspACI	CCGC	9 cut(s)	61, 138, 184, 238, 250, 265, 1606, 2192, 2754
BspANI	GGCC	6 cut(s)	197, 707, 1050, 1766, 2431, 2494
BspCNI	CTCAG	2 cut(s)	1750, 2563
BspFNI	CGCG	5 cut(s)	129, 186, 492, 528, 2192
BspHI	TCATGA	2 cut(s)	780, 2656
BspLI	GGNNCC	6 cut(s)	227, 395, 507, 1042, 1239, 1765
BspMI	ACCTGC	1 cut(s)	2528
BspOI	GCTAGC	1 cut(s)	2544
BspPI	GGATC	5 cut(s)	387, 1704, 1823, 2035, 2209
BspTI	CTTAAG	2 cut(s)	1711, 1729
BspTNI	GGTCTC	2 cut(s)	1364, 2311
BsrBI	CCGCTC	1 cut(s)	252
BsrDI	GCAATG	5 cut(s)	322, 391, 1653, 2029, 2679
BsrFI	RCCGGY	2 cut(s)	1512, 1978
BsrGI	TGTACA	2 cut(s)	1661, 2451
BsrI	ACTGG	5 cut(s)	1060, 1827, 2086, 2236, 2698
BssAI	RCCGGY	2 cut(s)	1512, 1978
BssECI	CCNNGG	7 cut(s)	501, 639, 856, 1329, 1501, 1677, 1678
BssNI	GRCGYC	1 cut(s)	465
BssT1I	CCWWGG	3 cut(s)	501, 856, 1501
Bst2UI	CCWGG	5 cut(s)	640, 816, 1242, 1331, 1679
Bst4CI	ACNGT	6 cut(s)	872, 1094, 1587, 1624, 1666, 1672
Bst6I	CTCTTC	4 cut(s)	44, 161, 2832, 2875
BstACI	GRCGYC	1 cut(s)	465
BstAFI	CTTAAG	2 cut(s)	1711, 1729
BstAUI	TGTACA	2 cut(s)	1661, 2451
BstC8I	GCNNGC	4 cut(s)	127, 430, 1200, 2542
BstDEI	CTNAG	7 cut(s)	1045, 1407, 1628, 1758, 2319, 2384, 2571
BstDSI	CCRYGG	1 cut(s)	856
BstEII	GGTNACC	2 cut(s)	1862, 1884
BstENI	CCTNNNNNAGG	1 cut(s)	700
BstF5I	GGATG	6 cut(s)	934, 1098, 1360, 1417, 1904, 2215
BstFNI	CGCG	5 cut(s)	129, 186, 492, 528, 2192
BstH2I	RGCGCY	2 cut(s)	262, 435
BstHHI	GCGC	5 cut(s)	131, 261, 434, 492, 530
BstMAI	GTCTC	4 cut(s)	1364, 2265, 2311, 2834
BstMCI	CGRYCG	1 cut(s)	184
BstMWI	GCNNNNNNNGC	8 cut(s)	137, 258, 1244, 1292, 1536, 1763, 2385, 2520
BstNI	CCWGG	5 cut(s)	640, 816, 1242, 1331, 1679
BstNSI	RCATGY	4 cut(s)	1662, 1725, 2379, 2719
BstPI	GGTNACC	2 cut(s)	1862, 1884
BstSCI	CCNGG	5 cut(s)	638, 814, 1240, 1329, 1677
BstSFI	CTRYAG	1 cut(s)	1027
BstSLI	GKGCMC	1 cut(s)	1677
BstUI	CGCG	5 cut(s)	129, 186, 492, 528, 2192
BstV1I	GCAGC	3 cut(s)	569, 572, 2845
BstV2I	GAAGAC	1 cut(s)	309
BstX2I	RGATCY	6 cut(s)	1369, 1696, 1828, 2027, 2201, 2554
BstXI	CCANNNNNNTGG	1 cut(s)	1307
BstYI	RGATCY	6 cut(s)	1369, 1696, 1828, 2027, 2201, 2554
Bsu36I	CCTNAGG	1 cut(s)	1045
BsuRI	GGCC	6 cut(s)	197, 707, 1050, 1766, 2431, 2494
BtgI	CCRYGG	1 cut(s)	856
BtrI	CACGTC	1 cut(s)	1942
BtsCI	GGATG	6 cut(s)	934, 1098, 1360, 1417, 1904, 2215
BtsI	GCAGTG	2 cut(s)	402, 1716
BtsIMutI	CAGTG	8 cut(s)	402, 969, 1099, 1260, 1312, 1671, 1677, 1716
BveI	ACCTGC	1 cut(s)	2528
Cac8I	GCNNGC	4 cut(s)	127, 430, 1200, 2542
CciI	TCATGA	2 cut(s)	780, 2656
CfoI	GCGC	5 cut(s)	131, 261, 434, 492, 530
Cfr10I	RCCGGY	2 cut(s)	1512, 1978
Cfr13I	GGNCC	7 cut(s)	226, 394, 505, 705, 1624, 1764, 2492
CseI	GACGC	3 cut(s)	197, 434, 2162
CspAI	ACCGGT	1 cut(s)	1512
CspCI	CAANNNNNGTGG	2 cut(s)	1167, 1202
DdeI	CTNAG	7 cut(s)	1045, 1407, 1628, 1758, 2319, 2384, 2571
DraI	TTTAAA	1 cut(s)	453
EaeI	YGGCCR	1 cut(s)	195
Eam1104I	CTCTTC	4 cut(s)	44, 161, 2832, 2875
EarI	CTCTTC	4 cut(s)	44, 161, 2832, 2875
EciI	GGCGGA	1 cut(s)	227
Eco130I	CCWWGG	3 cut(s)	501, 856, 1501
Eco147I	AGGCCT	2 cut(s)	1050, 2431
Eco24I	GRGCYC	1 cut(s)	1240
Eco31I	GGTCTC	2 cut(s)	1364, 2311
Eco47I	GGWCC	4 cut(s)	226, 394, 505, 1624
Eco57I	CTGAAG	1 cut(s)	2154
Eco81I	CCTNAGG	1 cut(s)	1045
Eco91I	GGTNACC	2 cut(s)	1862, 1884
EcoNI	CCTNNNNNAGG	1 cut(s)	700
EcoO109I	RGGNCCY	4 cut(s)	226, 394, 705, 1764
EcoO65I	GGTNACC	2 cut(s)	1862, 1884
EcoRII	CCWGG	5 cut(s)	638, 814, 1240, 1329, 1677
EcoT14I	CCWWGG	3 cut(s)	501, 856, 1501
EcoT22I	ATGCAT	1 cut(s)	2092
EcoT38I	GRGCYC	1 cut(s)	1240
ErhI	CCWWGG	3 cut(s)	501, 856, 1501
Esp3I	CGTCTC	1 cut(s)	2834
FalI	AAGNNNNNCTT	2 cut(s)	1182, 1214
FaqI	GGGAC	6 cut(s)	103, 239, 491, 764, 1654, 1958
FauI	CCCGC	1 cut(s)	272
FauNDI	CATATG	2 cut(s)	1935, 2863
FbaI	TGATCA	1 cut(s)	1851
FblI	GTMKAC	2 cut(s)	333, 1819
Fnu4HI	GCNGC	4 cut(s)	138, 558, 561, 2859
FokI	GGATG	6 cut(s)	941, 1085, 1367, 1424, 1891, 2222
FriOI	GRGCYC	1 cut(s)	1240
Fsp4HI	GCNGC	4 cut(s)	138, 558, 561, 2859
FspBI	CTAG	7 cut(s)	149, 1061, 1142, 1181, 2489, 2541, 2630
GlaI	GCGC	5 cut(s)	130, 260, 433, 491, 529
GluI	GCNGC	4 cut(s)	138, 558, 561, 2859
GsaI	CCCAGC	1 cut(s)	259
GsuI	CTGGAG	2 cut(s)	1224, 2103
HaeII	RGCGCY	2 cut(s)	262, 435
HaeIII	GGCC	6 cut(s)	197, 707, 1050, 1766, 2431, 2494
HapII	CCGG	4 cut(s)	1153, 1513, 1979, 2396
HgaI	GACGC	3 cut(s)	197, 434, 2162
HhaI	GCGC	5 cut(s)	131, 261, 434, 492, 530
Hin1I	GRCGYC	1 cut(s)	465
Hin6I	GCGC	5 cut(s)	129, 259, 432, 490, 528
HinP1I	GCGC	5 cut(s)	129, 259, 432, 490, 528
HpaII	CCGG	4 cut(s)	1153, 1513, 1979, 2396
HphI	GGTGA	3 cut(s)	661, 1454, 1874
Hpy166II	GTNNAC	3 cut(s)	334, 1820, 2333
Hpy188III	TCNNGA	7 cut(s)	781, 1427, 2050, 2435, 2657, 2663, 2848
Hpy8I	GTNNAC	3 cut(s)	334, 1820, 2333
HpyCH4III	ACNGT	6 cut(s)	872, 1094, 1587, 1624, 1666, 1672
HpyCH4IV	ACGT	6 cut(s)	306, 465, 518, 573, 1919, 1941
HpyF10VI	GCNNNNNNNGC	8 cut(s)	137, 258, 1244, 1292, 1536, 1763, 2385, 2520
HpyF3I	CTNAG	7 cut(s)	1045, 1407, 1628, 1758, 2319, 2384, 2571
HpySE526I	ACGT	6 cut(s)	306, 465, 518, 573, 1919, 1941
Hsp92I	GRCGYC	1 cut(s)	465
HspAI	GCGC	5 cut(s)	129, 259, 432, 490, 528
Ksp22I	TGATCA	1 cut(s)	1851
LmnI	GCTCC	6 cut(s)	30, 108, 257, 1243, 1300, 2261
Lsp1109I	GCAGC	3 cut(s)	569, 572, 2845
LweI	GCATC	3 cut(s)	2064, 2077, 2173
MaeI	CTAG	7 cut(s)	149, 1061, 1142, 1181, 2489, 2541, 2630
MaeII	ACGT	6 cut(s)	306, 465, 518, 573, 1919, 1941
MaeIII	GTNAC	8 cut(s)	1255, 1587, 1666, 1862, 1884, 1915, 2039, 2759
MbiI	CCGCTC	1 cut(s)	252
MflI	RGATCY	6 cut(s)	1369, 1696, 1828, 2027, 2201, 2554
MhlI	GDGCHC	2 cut(s)	1240, 1677
MlyI	GAGTC	6 cut(s)	209, 846, 926, 1252, 1472, 1910
MmeI	TCCRAC	4 cut(s)	52, 198, 1286, 1332
Mph1103I	ATGCAT	1 cut(s)	2092
MroXI	GAANNNNTTC	1 cut(s)	2597
MseI	TTAA	8 cut(s)	452, 599, 795, 954, 992, 1712, 1730, 2619
MslI	CAYNNNNRTG	2 cut(s)	1657, 2742
MspA1I	CMGCKG	1 cut(s)	2858
MspCI	CTTAAG	2 cut(s)	1711, 1729
MspI	CCGG	4 cut(s)	1153, 1513, 1979, 2396
MspR9I	CCNGG	5 cut(s)	640, 816, 1242, 1331, 1679
Mva1269I	GAATGC	1 cut(s)	1783
MvaI	CCWGG	5 cut(s)	640, 816, 1242, 1331, 1679
MvnI	CGCG	5 cut(s)	129, 186, 492, 528, 2192
MwoI	GCNNNNNNNGC	8 cut(s)	137, 258, 1244, 1292, 1536, 1763, 2385, 2520
NcoI	CCATGG	1 cut(s)	856
NdeI	CATATG	2 cut(s)	1935, 2863
NheI	GCTAGC	1 cut(s)	2540
NlaIV	GGNNCC	6 cut(s)	227, 395, 507, 1042, 1239, 1765
NmuCI	GTSAC	4 cut(s)	1255, 1666, 1862, 2759
NsiI	ATGCAT	1 cut(s)	2092
NspI	RCATGY	4 cut(s)	1662, 1725, 2379, 2719
OliI	CACNNNNGTG	1 cut(s)	2742
PagI	TCATGA	2 cut(s)	780, 2656
PasI	CCCWGGG	1 cut(s)	1678
PceI	AGGCCT	2 cut(s)	1050, 2431
PciI	ACATGT	2 cut(s)	1658, 2715
PcsI	WCGNNNNNNNCGW	3 cut(s)	195, 279, 297
PctI	GAATGC	1 cut(s)	1783
PdmI	GAANNNNTTC	1 cut(s)	2597
PflMI	CCANNNNNTGG	1 cut(s)	1108
PfoI	TCCNGGA	1 cut(s)	814
PinAI	ACCGGT	1 cut(s)	1512
PkrI	GCNGC	4 cut(s)	139, 559, 562, 2860
PleI	GAGTC	6 cut(s)	209, 846, 925, 1252, 1472, 1909
PpsI	GAGTC	6 cut(s)	209, 846, 925, 1252, 1472, 1909
PpuMI	RGGWCCY	2 cut(s)	226, 394
PscI	ACATGT	2 cut(s)	1658, 2715
Psp5II	RGGWCCY	2 cut(s)	226, 394
Psp6I	CCWGG	5 cut(s)	638, 814, 1240, 1329, 1677
PspEI	GGTNACC	2 cut(s)	1862, 1884
PspFI	CCCAGC	1 cut(s)	255
PspGI	CCWGG	5 cut(s)	638, 814, 1240, 1329, 1677
PspN4I	GGNNCC	6 cut(s)	227, 395, 507, 1042, 1239, 1765
PspPI	GGNCC	7 cut(s)	226, 394, 505, 705, 1624, 1764, 2492
PspPPI	RGGWCCY	2 cut(s)	226, 394
PsrI	GAACNNNNNNTAC	2 cut(s)	865, 897
PsuI	RGATCY	6 cut(s)	1369, 1696, 1828, 2027, 2201, 2554
PvuII	CAGCTG	1 cut(s)	2858
RseI	CAYNNNNRTG	2 cut(s)	1657, 2742
SaqAI	TTAA	8 cut(s)	452, 599, 795, 954, 992, 1712, 1730, 2619
SatI	GCNGC	4 cut(s)	138, 558, 561, 2859
Sau96I	GGNCC	7 cut(s)	226, 394, 505, 705, 1624, 1764, 2492
ScaI	AGTACT	1 cut(s)	2058
SchI	GAGTC	6 cut(s)	209, 846, 926, 1252, 1472, 1910
ScrFI	CCNGG	5 cut(s)	640, 816, 1242, 1331, 1679
SduI	GDGCHC	2 cut(s)	1240, 1677
SfaNI	GCATC	3 cut(s)	2064, 2077, 2173
SfcI	CTRYAG	1 cut(s)	1027
SgrAI	CRCCGGYG	1 cut(s)	1978
SinI	GGWCC	4 cut(s)	226, 394, 505, 1624
SmiMI	CAYNNNNRTG	2 cut(s)	1657, 2742
SmlI	CTYRAG	3 cut(s)	1711, 1729, 2433
SmoI	CTYRAG	3 cut(s)	1711, 1729, 2433
SseBI	AGGCCT	2 cut(s)	1050, 2431
SsiI	CCGC	9 cut(s)	61, 138, 184, 238, 250, 265, 1606, 2192, 2754
SspI	AATATT	1 cut(s)	1451
SspMI	CTAG	7 cut(s)	149, 1061, 1142, 1181, 2489, 2541, 2630
StuI	AGGCCT	2 cut(s)	1050, 2431
StyD4I	CCNGG	5 cut(s)	638, 814, 1240, 1329, 1677
StyI	CCWWGG	3 cut(s)	501, 856, 1501
TaaI	ACNGT	6 cut(s)	872, 1094, 1587, 1624, 1666, 1672
TaiI	ACGT	6 cut(s)	309, 468, 521, 576, 1922, 1944
TaqI	TCGA	2 cut(s)	231, 1491
TatI	WGTACW	5 cut(s)	1631, 1661, 2056, 2332, 2451
TauI	GCSGC	1 cut(s)	140
Tru1I	TTAA	8 cut(s)	452, 599, 795, 954, 992, 1712, 1730, 2619
Tru9I	TTAA	8 cut(s)	452, 599, 795, 954, 992, 1712, 1730, 2619
TscAI	CASTG	8 cut(s)	409, 976, 1099, 1260, 1312, 1671, 1677, 1723
TseFI	GTSAC	4 cut(s)	1255, 1666, 1862, 2759
TseI	GCWGC	3 cut(s)	557, 560, 2858
Tsp45I	GTSAC	4 cut(s)	1255, 1666, 1862, 2759
TspGWI	ACGGA	3 cut(s)	386, 428, 726
TspRI	CASTG	8 cut(s)	409, 976, 1099, 1260, 1312, 1671, 1677, 1723
Van91I	CCANNNNNTGG	1 cut(s)	1108
Vha464I	CTTAAG	2 cut(s)	1711, 1729
VpaK11BI	GGWCC	4 cut(s)	226, 394, 505, 1624
XagI	CCTNNNNNAGG	1 cut(s)	700
XapI	RAATTY	2 cut(s)	649, 2400
XceI	RCATGY	4 cut(s)	1662, 1725, 2379, 2719
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1105, 2502
XmiI	GTMKAC	2 cut(s)	333, 1819
XmnI	GAANNNNTTC	1 cut(s)	2597
XspI	CTAG	7 cut(s)	149, 1061, 1142, 1181, 2489, 2541, 2630
ZraI	GACGTC	1 cut(s)	466
ZrmI	AGTACT	1 cut(s)	2058
Zsp2I	ATGCAT	1 cut(s)	2092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.