Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0006941

Physical Location & Seq

Genomic Coordinates Forward (+)

21053547 .. 21061641

Gene Structure 8095 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G018760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2838 bp

ATGACCCAACTCTCGGAAAAGCTCCGTCTGTGTCTTCTCCTTCTTCTGTACAATCCAACCCGGTTTCTCTCTCCTCCTAAGCAGCTCTTGGCCACTCTCTCCGTCTCCACGCTCCACCTCTGCAAGTCCCTCGCACGCGCCCTCGCCTCCAAAACCCTAGCCCTCTTCTTCTTCTTCTTGTTCTTCTTCGCCATGGACGACCCCTTCCCCTTCGCCTCTGCCGACGGCGACAACGACGGCTGGGACTTCGACCTCCAGTCCCGCAACCGCGCCCAGCGCCCCCGCCTGCTCCACGACTACGACGACGACGACGACGTTTCCGACAATGCCGTCGATAAGGTCTACCTCGTTCCCTACAGGTGGTGGAAGGAGGTTGGGACCGAAGACGATGATCGTGGCGAAGGGGTATTGTACAGCACTTCGCCGGAGGAAGACGACTCCGGCTCGGAAATTGTGTTGAGTTTGAGGAAGAGGGACTCTGCTGAAGAAGGGTTTTCAGGTCGCGAGTATGCCTTGGTCCCTGAAGCGACATGGTGCCGCGCACTCATACGGCATTATGATTTTAATACAGCAGCAATGGATAATGTGAGCTTCTTTGGTGACGAAGAGTTTTTAAAGGATGTCTTTTCTTTACAAATTAGGCTCTTTGTTTCCTGGGAAACAAATTCATTGGTTGTAAAGATCAGCCAAAAGGATAATGCGGTTGATTCTTTCAAGAGAGCCTGCATTATTTTCAGTTCAGAGCCCGAGCCATTGCATATTTGGGACTTCTCAGGGCAGACAACCCAATTTTTCATGAATGACAGAATTAGTTTGCCAAATGACATTCCTGGACAACTGAACAAAGAGATTGTCCTGGAATTGCATCTTCATGGATTTTCAGAGGTCAGAGGTTGGAGGAGTGATGACATGGCTGAAAACCACTTCAGTATTGATGGTTGTATTAGTGGTGGCTCTTCTAAAATGAATGGGAACATTTACTACGTCAATCCCTACTTAAACTCAAGCAACTCCTGGCGAAGTGCTGTTATGTATAGAGGAGTTGGTTCTTTAGGCTTAACTGGTATAAAGAATCTTGGGAATACTTGTTTTATGAACAGTGCAATCCAATGTTTGGTGCACACTCCAAAGCTTGTTGACTATTTTCTAGGAGATTACCGGAAGGACATGAACTATCAGAACCCATTGGGAATGAAAGGAGAGCTTGCTTTTGCATTTGGAGAATTATTGAGGAGGTTATGGGCTCCAGGCGCAAGGCCAGTGGCTCCAAGAATGTTCAAGCAAAAACTTGCCAGTTTTGCTCCTCAATTCAGTGGCTATAATCAACATGATTCCCAGGAACTTCTTGCTTTTTTGCTGGATGGTCTCCATGAAGATCTAAATCGTGTGAAATGCAAGCCATATTTTGAAGCTAGAGATGCAGAGGGCCGTCCAGATGAAGAAGTGGCGGAAGAATATTGGCGGAATCACCTTGCTCGCAATGATTCCATTATTGTTAATGCATACCAGGGTCAATACCGGTCAAAATTGGTTTGCCCCGATTGCAACAAGGTGTCCATTACATTTGACCCATTTATGTATCTATCACTGCCATTACCTTCTACAACAATGCGGAGTATGACTTTGACGGTTTTAAGCACAGATGGTGCTGCGTTGCCTACTACATTTACTGTAACAGTGCCAAAGAGTGGGAAGCTCGTGGATCTTACTGAAGCCTTAAGAATTGCCTGTTCTCTAAGAGATGATGAAACCCTGTTGGTGGCTGAGATATACCAGTGTCGCATATTTCGTCTATTGGAGGATCCATCTGATTCATTAGCCTTTGTTAGAGACGCTGACAAACTGGTTGCTTATCGCTTACCAAAAGATTTTGAGCGATCGCACTTGGTTGTGTTTCTTCATCAGGAGAAGGGAAAGTATTACTATAATCAAGACACAATTGGTATCCCACTTTTAGCAAGGGTGTCTGATCCTTGTTATGGATCTGATATTCGCAACGAATTCCTAAATAATTTAAATCCATTCTTGAAGCCTGATGGAGATATATCAGATGTTTTTGATGATGATTCTGGAAACTGTGCTAATGAAGACACTAACAAGGAGGATGACAGTACTACAGTTTGGGATAATGATTCTGACACAGACAGGGGAACAATGGGTGAAGTGCTGTTGGATACTGACTTCAAATTTTACTTAGCAGATACAAGAAAGGAAATAGAGTTGAATCAGCTGATAGAGGTCTCACGGTTGCCTGTGATACCAAGGCCTAAGATACTGGAGGTGATTGTTTTATGGACAGATGAGATGATTAACAAGTATGATACACGCCTTCTAAACTCTTTGCCTGAGGTCTTCAACATTTTCACGAGTAGTGAAGAATCTGTTTCCCTGTACAAATGCCTTGAGGCCTTTCTGAAGGAAGAACCTCTAGGACCTGAAGACATGTGGTTTTGCCCTAGCTGCAAGACTCCTCGGCAAGCCAGCAAAAAGTTAGATCTTTGGAGATTGCCTGAGATATTGGTTATTCATCTGAAAAGATTCTCGTATGACCATTATTTTAAGAACAAGCTAGAAACATTAGTTGATTTTCCCATTTATGATATGGACATCTCAACATACATTACCCACCGCAGTAACCAGTTATCCAGTCGATACATGTTGTACGCAATCAGTAATCACTATGGTGGCTTGGGTGGTGGTCACTATACGGCATTTGTTCATCTCGGTAAGGGTTCATGGTATGAGTTTGATGATGACAGAGTTTCTGCTGTTAATGTGGAGAGGATTAAGACTTCGGCAGCATATGTTCTTTTCTACCGAAGAGTACAAGATGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

UCH

Protein Analysis

945

Amino Acids

108.24

Weight (kDa)

4.98

Isoelectric Point (pI)

40.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubiquitin_UBP8	PF25242	213 - 267	1.5e-30	UBP8-like, ubiquitin-like domain
UCH	PF00443	354 - 939	1.7e-73	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006941)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g20680 FvH4_6g20680 FvH4_6g20680
malus_domestica	MD00G1072700.v1.1 MD12G1043100.v1.1 MD14G1042200.v1.1
prunus_persica	Prupe.7G091700_v2.0.a1 Prupe.7G091700_v2.0.a1 Prupe.7G091700_v2.0.a1 Prupe.7G091700_v2.0.a1
pyrus_communis	pycom12g03720 pycom14g03580
rosa_chinensis	RchiOBHm_Chr3g0476581
rosa_laevigata	RLG00000001984 RLG00000023761
rosa_multiflora	Rmu_sc0008241.1_g000007 Rmu_sc0020945.1_g000001
rosa_roxburghii	Rroxscaffold_6G00405190
rosa_rugosa	Rorug03G0155400
rosa_samantha	Rh3AG206000 Rh3BG238600 Rh3CG232300 Rh3DG232200 Rh7DG310400
rosa_wichuraiana	Rw3G018760 Rw7G026340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	329
AccB1I	GGYRCC	1 cut(s)	534
AccB7I	CCANNNNNTGG	2 cut(s)	1114, 1688
AccI	GTMKAC	1 cut(s)	342
AccII	CGCG	4 cut(s)	138, 270, 504, 540
AciI	CCGC	9 cut(s)	262, 268, 283, 538, 701, 1448, 1462, 1612, 2629
AclWI	GGATC	5 cut(s)	1710, 1796, 1809, 1964, 1990
AcoI	YGGCCR	1 cut(s)	90
AcsI	RAATTY	3 cut(s)	664, 2000, 2186
AcuI	CTGAAG	6 cut(s)	504, 543, 910, 1731, 2435, 2457
AfaI	GTAC	6 cut(s)	50, 413, 2113, 2393, 2663, 2826
AfiI	CCNNNNNNNGG	6 cut(s)	13, 360, 1114, 1688, 1759, 1979
AflII	CTTAAG	1 cut(s)	1717
AflIII	ACRYGT	2 cut(s)	2442, 2655
AgeI	ACCGGT	1 cut(s)	1518
AgsI	TTSAA	7 cut(s)	715, 1279, 1409, 2029, 2185, 2224, 2356
AhdI	GACNNNNNGTC	1 cut(s)	256
AjnI	CCWGG	7 cut(s)	653, 829, 855, 1013, 1246, 1335, 1506
AleI	CACNNNNGTG	1 cut(s)	2682
Alw21I	GWGCWC	1 cut(s)	1122
Alw26I	GTCTC	4 cut(s)	109, 1370, 1824, 2245
Alw44I	GTGCAC	1 cut(s)	1118
AlwI	GGATC	5 cut(s)	1710, 1796, 1809, 1964, 1990
Ama87I	CYCGRG	1 cut(s)	746
AoxI	GGCC	5 cut(s)	90, 1256, 1426, 2264, 2406
ApaLI	GTGCAC	1 cut(s)	1118
ApeKI	GCWGC	5 cut(s)	82, 572, 1649, 2460, 2798
ApoI	RAATTY	3 cut(s)	664, 2000, 2186
ArsI	GACNNNNNNTTYG	2 cut(s)	837, 869
AsiGI	ACCGGT	1 cut(s)	1518
AsiSI	GCGATCGC	1 cut(s)	1880
Asp700I	GAANNNNTTC	1 cut(s)	2537
AspLEI	GCGC	5 cut(s)	140, 272, 279, 542, 1253
AspS9I	GGNCC	4 cut(s)	378, 517, 1426, 2432
AsuC2I	CCSGG	1 cut(s)	61
AsuHPI	GGTGA	4 cut(s)	611, 1460, 2171, 2293
AvaI	CYCGRG	1 cut(s)	746
AvaII	GGWCC	3 cut(s)	378, 517, 2432
AxyI	CCTNAGG	1 cut(s)	2346
BaeGI	GKGCMC	1 cut(s)	1122
BalI	TGGCCA	1 cut(s)	92
BamHI	GGATCC	1 cut(s)	1801
BanI	GGYRCC	1 cut(s)	534
BanII	GRGCYC	2 cut(s)	747, 1246
BauI	CACGAG	2 cut(s)	1697, 2365
BbsI	GAAGAC	6 cut(s)	26, 390, 438, 2094, 2344, 2445
Bbv12I	GWGCWC	1 cut(s)	1122
BbvI	GCAGC	5 cut(s)	94, 584, 1636, 2447, 2810
BccI	CCATC	5 cut(s)	929, 1355, 1637, 1813, 2030
BceAI	ACGGC	6 cut(s)	241, 253, 314, 566, 1413, 2724
BcgI	CGANNNNNNTGC	2 cut(s)	112, 146
BciT130I	CCWGG	7 cut(s)	655, 831, 857, 1015, 1248, 1337, 1508
BciVI	GTATCC	2 cut(s)	1955, 2167
BcnI	CCSGG	1 cut(s)	61
BcoDI	GTCTC	4 cut(s)	109, 1370, 1824, 2245
BfaI	CTAG	6 cut(s)	158, 1148, 1413, 2429, 2457, 2570
BfmI	CTRYAG	2 cut(s)	355, 2115
BfoI	RGCGCY	1 cut(s)	280
BfrI	CTTAAG	1 cut(s)	1717
BfuI	GTATCC	2 cut(s)	1955, 2167
BglII	AGATCT	2 cut(s)	1375, 2494
BisI	GCNGC	6 cut(s)	83, 538, 573, 1650, 2461, 2799
BlsI	GCNGC	6 cut(s)	84, 539, 574, 1651, 2462, 2800
BmcAI	AGTACT	1 cut(s)	2113
Bme1390I	CCNGG	8 cut(s)	61, 655, 831, 857, 1015, 1248, 1337, 1508
Bme18I	GGWCC	3 cut(s)	378, 517, 2432
BmeRI	GACNNNNNGTC	1 cut(s)	256
BmeT110I	CYCGRG	1 cut(s)	746
BmgT120I	GGNCC	4 cut(s)	378, 517, 1426, 2432
BmiI	GGNNCC	6 cut(s)	379, 519, 536, 1245, 1266, 1803
BmrFI	CCNGG	8 cut(s)	61, 655, 831, 857, 1015, 1248, 1337, 1508
BmsI	GCATC	2 cut(s)	874, 1408
BpiI	GAAGAC	6 cut(s)	26, 390, 438, 2094, 2344, 2445
BpmI	CTGGAG	3 cut(s)	239, 1230, 2297
Bpu10I	CCTNAGC	1 cut(s)	78
BpuEI	CTTGAG	2 cut(s)	988, 2423
BpuMI	CCSGG	1 cut(s)	61
BsaBI	GATNNNNATC	1 cut(s)	1380
BsaI	GGTCTC	2 cut(s)	1370, 2245
BsaJI	CCNNGG	7 cut(s)	192, 513, 654, 1335, 1507, 2261, 2471
BsaWI	WCCGGW	2 cut(s)	1158, 1518
Bsc4I	CCNNNNNNNGG	6 cut(s)	13, 360, 1114, 1688, 1759, 1979
Bse118I	RCCGGY	1 cut(s)	1518
Bse1I	ACTGG	9 cut(s)	256, 1066, 1259, 1293, 1774, 1848, 2280, 2638, 2646
Bse21I	CCTNAGG	1 cut(s)	2346
Bse3DI	GCAATG	3 cut(s)	582, 752, 1486
Bse8I	GATNNNNATC	1 cut(s)	1380
BseBI	CCWGG	7 cut(s)	655, 831, 857, 1015, 1248, 1337, 1508
BseDI	CCNNGG	7 cut(s)	192, 513, 654, 1335, 1507, 2261, 2471
BseGI	GGATG	3 cut(s)	625, 1366, 2110
BseJI	GATNNNNATC	1 cut(s)	1380
BseLI	CCNNNNNNNGG	6 cut(s)	13, 360, 1114, 1688, 1759, 1979
BseMI	GCAATG	3 cut(s)	582, 752, 1486
BseMII	CTCAG	4 cut(s)	786, 1755, 2337, 2502
BseNI	ACTGG	9 cut(s)	256, 1066, 1259, 1293, 1774, 1848, 2280, 2638, 2646
BseRI	GAGGAG	6 cut(s)	63, 913, 1053, 1246, 1293, 2460
BseSI	GKGCMC	1 cut(s)	1122
BseXI	GCAGC	5 cut(s)	94, 584, 1636, 2447, 2810
BseYI	CCCAGC	2 cut(s)	240, 273
Bsh1236I	CGCG	4 cut(s)	138, 270, 504, 540
Bsh1285I	CGRYCG	1 cut(s)	1880
BshFI	GGCC	5 cut(s)	92, 1258, 1428, 2266, 2408
BshNI	GGYRCC	1 cut(s)	534
BshTI	ACCGGT	1 cut(s)	1518
BsiEI	CGRYCG	1 cut(s)	1880
BsiHKAI	GWGCWC	1 cut(s)	1122
BsiHKCI	CYCGRG	1 cut(s)	746
BsiSI	CCGG	5 cut(s)	61, 425, 441, 1159, 1519
BslFI	GGGAC	7 cut(s)	112, 244, 257, 391, 488, 503, 779
BslI	CCNNNNNNNGG	6 cut(s)	13, 360, 1114, 1688, 1759, 1979
BsmAI	GTCTC	4 cut(s)	109, 1370, 1824, 2245
BsmBI	CGTCTC	2 cut(s)	109, 1824
BsmFI	GGGAC	7 cut(s)	112, 244, 257, 391, 488, 503, 779
BsnI	GGCC	5 cut(s)	92, 1258, 1428, 2266, 2408
Bso31I	GGTCTC	2 cut(s)	1370, 2245
BsoBI	CYCGRG	1 cut(s)	746
Bsp1286I	GDGCHC	3 cut(s)	747, 1122, 1246
Bsp1407I	TGTACA	3 cut(s)	48, 411, 2391
Bsp143I	GATC	9 cut(s)	391, 681, 1375, 1702, 1801, 1877, 1969, 1982, 2494
Bsp19I	CCATGG	1 cut(s)	192
Bsp68I	TCGCGA	1 cut(s)	504
BspACI	CCGC	9 cut(s)	262, 268, 283, 538, 701, 1448, 1462, 1612, 2629
BspANI	GGCC	5 cut(s)	92, 1258, 1428, 2266, 2408
BspCNI	CTCAG	4 cut(s)	785, 1756, 2338, 2503
BspFNI	CGCG	4 cut(s)	138, 270, 504, 540
BspHI	TCATGA	1 cut(s)	795
BspLI	GGNNCC	6 cut(s)	379, 519, 536, 1245, 1266, 1803
BspPI	GGATC	5 cut(s)	1710, 1796, 1809, 1964, 1990
BspQI	GCTCTTC	1 cut(s)	961
BspT107I	GGYRCC	1 cut(s)	534
BspTI	CTTAAG	1 cut(s)	1717
BspTNI	GGTCTC	2 cut(s)	1370, 2245
BsrDI	GCAATG	3 cut(s)	582, 752, 1486
BsrFI	RCCGGY	1 cut(s)	1518
BsrGI	TGTACA	3 cut(s)	48, 411, 2391
BsrI	ACTGG	9 cut(s)	256, 1066, 1259, 1293, 1774, 1848, 2280, 2638, 2646
BssAI	RCCGGY	1 cut(s)	1518
BssECI	CCNNGG	7 cut(s)	192, 513, 654, 1335, 1507, 2261, 2471
BssMI	GATC	9 cut(s)	391, 681, 1375, 1702, 1801, 1877, 1969, 1982, 2494
BssSI	CACGAG	2 cut(s)	1697, 2365
BssT1I	CCWWGG	3 cut(s)	192, 513, 2261
Bst2BI	CACGAG	2 cut(s)	1697, 2365
Bst2UI	CCWGG	7 cut(s)	655, 831, 857, 1015, 1248, 1337, 1508
Bst4CI	ACNGT	8 cut(s)	1100, 1630, 1672, 1678, 2078, 2111, 2119, 2247
Bst6I	CTCTTC	5 cut(s)	170, 464, 600, 961, 2815
BstAFI	CTTAAG	1 cut(s)	1717
BstAUI	TGTACA	3 cut(s)	48, 411, 2391
BstC8I	GCNNGC	8 cut(s)	136, 287, 724, 1206, 1397, 1477, 2478, 2482
BstDEI	CTNAG	8 cut(s)	78, 772, 1736, 1764, 2194, 2268, 2346, 2511
BstDSI	CCRYGG	1 cut(s)	192
BstF5I	GGATG	3 cut(s)	625, 1366, 2110
BstFNI	CGCG	4 cut(s)	138, 270, 504, 540
BstH2I	RGCGCY	1 cut(s)	280
BstHHI	GCGC	5 cut(s)	140, 272, 279, 542, 1253
BstKTI	GATC	9 cut(s)	394, 684, 1378, 1705, 1804, 1880, 1972, 1985, 2497
BstMAI	GTCTC	4 cut(s)	109, 1370, 1824, 2245
BstMBI	GATC	9 cut(s)	391, 681, 1375, 1702, 1801, 1877, 1969, 1982, 2494
BstMCI	CGRYCG	1 cut(s)	1880
BstMWI	GCNNNNNNNGC	6 cut(s)	276, 1250, 1298, 1418, 1542, 2460
BstNI	CCWGG	7 cut(s)	655, 831, 857, 1015, 1248, 1337, 1508
BstNSI	RCATGY	2 cut(s)	2446, 2659
BstSCI	CCNGG	8 cut(s)	59, 653, 829, 855, 1013, 1246, 1335, 1506
BstSFI	CTRYAG	2 cut(s)	355, 2115
BstSLI	GKGCMC	1 cut(s)	1122
BstUI	CGCG	4 cut(s)	138, 270, 504, 540
BstV1I	GCAGC	5 cut(s)	94, 584, 1636, 2447, 2810
BstV2I	GAAGAC	6 cut(s)	26, 390, 438, 2094, 2344, 2445
BstX2I	RGATCY	5 cut(s)	1375, 1702, 1801, 1982, 2494
BstYI	RGATCY	5 cut(s)	1375, 1702, 1801, 1982, 2494
Bsu36I	CCTNAGG	1 cut(s)	2346
BsuI	GTATCC	2 cut(s)	1955, 2167
BsuRI	GGCC	5 cut(s)	92, 1258, 1428, 2266, 2408
BtgI	CCRYGG	1 cut(s)	192
BtsCI	GGATG	3 cut(s)	625, 1366, 2110
BtsI	GCAGTG	1 cut(s)	1586
BtsIMutI	CAGTG	6 cut(s)	1105, 1266, 1318, 1586, 1683, 1781
BtuMI	TCGCGA	1 cut(s)	504
Cac8I	GCNNGC	8 cut(s)	136, 287, 724, 1206, 1397, 1477, 2478, 2482
CciI	TCATGA	1 cut(s)	795
CfoI	GCGC	5 cut(s)	140, 272, 279, 542, 1253
Cfr10I	RCCGGY	1 cut(s)	1518
Cfr13I	GGNCC	4 cut(s)	378, 517, 1426, 2432
CseI	GACGC	1 cut(s)	1841
Csp6I	GTAC	6 cut(s)	49, 412, 2112, 2392, 2662, 2825
CspAI	ACCGGT	1 cut(s)	1518
CspCI	CAANNNNNGTGG	2 cut(s)	1242, 1277
CviQI	GTAC	6 cut(s)	49, 412, 2112, 2392, 2662, 2825
DdeI	CTNAG	8 cut(s)	78, 772, 1736, 1764, 2194, 2268, 2346, 2511
DpnI	GATC	9 cut(s)	393, 683, 1377, 1704, 1803, 1879, 1971, 1984, 2496
DpnII	GATC	9 cut(s)	391, 681, 1375, 1702, 1801, 1877, 1969, 1982, 2494
DraI	TTTAAA	2 cut(s)	615, 2016
DrdI	GACNNNNNNGTC	1 cut(s)	329
DriI	GACNNNNNGTC	1 cut(s)	256
DseDI	GACNNNNNNGTC	1 cut(s)	329
EaeI	YGGCCR	1 cut(s)	90
Eam1104I	CTCTTC	5 cut(s)	170, 464, 600, 961, 2815
Eam1105I	GACNNNNNGTC	1 cut(s)	256
EarI	CTCTTC	5 cut(s)	170, 464, 600, 961, 2815
EciI	GGCGGA	2 cut(s)	1463, 1477
Eco130I	CCWWGG	3 cut(s)	192, 513, 2261
Eco147I	AGGCCT	2 cut(s)	2266, 2408
Eco24I	GRGCYC	2 cut(s)	747, 1246
Eco31I	GGTCTC	2 cut(s)	1370, 2245
Eco47I	GGWCC	3 cut(s)	378, 517, 2432
Eco57I	CTGAAG	6 cut(s)	504, 543, 910, 1731, 2435, 2457
Eco81I	CCTNAGG	1 cut(s)	2346
Eco88I	CYCGRG	1 cut(s)	746
EcoO109I	RGGNCCY	1 cut(s)	2432
EcoRI	GAATTC	1 cut(s)	2000
EcoRII	CCWGG	7 cut(s)	653, 829, 855, 1013, 1246, 1335, 1506
EcoT14I	CCWWGG	3 cut(s)	192, 513, 2261
EcoT22I	ATGCAT	1 cut(s)	1504
EcoT38I	GRGCYC	2 cut(s)	747, 1246
ErhI	CCWWGG	3 cut(s)	192, 513, 2261
Esp3I	CGTCTC	2 cut(s)	109, 1824
FalI	AAGNNNNNCTT	6 cut(s)	71, 103, 608, 640, 1188, 1220
FaqI	GGGAC	7 cut(s)	112, 244, 257, 391, 488, 503, 779
FauI	CCCGC	2 cut(s)	269, 290
FauNDI	CATATG	1 cut(s)	2803
FblI	GTMKAC	1 cut(s)	342
Fnu4HI	GCNGC	6 cut(s)	83, 538, 573, 1650, 2461, 2799
FokI	GGATG	3 cut(s)	632, 1373, 2117
FriOI	GRGCYC	2 cut(s)	747, 1246
Fsp4HI	GCNGC	6 cut(s)	83, 538, 573, 1650, 2461, 2799
FspBI	CTAG	6 cut(s)	158, 1148, 1413, 2429, 2457, 2570
GlaI	GCGC	5 cut(s)	139, 271, 278, 541, 1252
GluI	GCNGC	6 cut(s)	83, 538, 573, 1650, 2461, 2799
GsaI	CCCAGC	2 cut(s)	244, 277
GsuI	CTGGAG	3 cut(s)	239, 1230, 2297
HaeII	RGCGCY	1 cut(s)	280
HaeIII	GGCC	5 cut(s)	92, 1258, 1428, 2266, 2408
HapII	CCGG	5 cut(s)	61, 425, 441, 1159, 1519
HgaI	GACGC	1 cut(s)	1841
HhaI	GCGC	5 cut(s)	140, 272, 279, 542, 1253
Hin6I	GCGC	5 cut(s)	138, 270, 277, 540, 1251
HinP1I	GCGC	5 cut(s)	138, 270, 277, 540, 1251
HincII	GTYRAC	1 cut(s)	1138
HindII	GTYRAC	1 cut(s)	1138
HindIII	AAGCTT	1 cut(s)	1130
HpaII	CCGG	5 cut(s)	61, 425, 441, 1159, 1519
HphI	GGTGA	4 cut(s)	611, 1460, 2171, 2293
Hpy166II	GTNNAC	3 cut(s)	343, 1120, 1138
Hpy188III	TCNNGA	9 cut(s)	503, 715, 796, 1433, 1904, 1931, 2026, 2070, 2365
Hpy8I	GTNNAC	3 cut(s)	343, 1120, 1138
Hpy99I	CGWCG	8 cut(s)	227, 239, 305, 308, 311, 314, 317, 335
HpyCH4III	ACNGT	8 cut(s)	1100, 1630, 1672, 1678, 2078, 2111, 2119, 2247
HpyCH4IV	ACGT	2 cut(s)	315, 984
HpyF10VI	GCNNNNNNNGC	6 cut(s)	276, 1250, 1298, 1418, 1542, 2460
HpyF3I	CTNAG	8 cut(s)	78, 772, 1736, 1764, 2194, 2268, 2346, 2511
HpySE526I	ACGT	2 cut(s)	315, 984
HspAI	GCGC	5 cut(s)	138, 270, 277, 540, 1251
Kzo9I	GATC	9 cut(s)	391, 681, 1375, 1702, 1801, 1877, 1969, 1982, 2494
LguI	GCTCTTC	1 cut(s)	961
LmnI	GCTCC	6 cut(s)	27, 117, 294, 1249, 1270, 1306
Lsp1109I	GCAGC	5 cut(s)	94, 584, 1636, 2447, 2810
LweI	GCATC	2 cut(s)	874, 1408
MaeI	CTAG	6 cut(s)	158, 1148, 1413, 2429, 2457, 2570
MaeII	ACGT	2 cut(s)	315, 984
MaeIII	GTNAC	4 cut(s)	599, 1672, 2633, 2699
MalI	GATC	9 cut(s)	393, 683, 1377, 1704, 1803, 1879, 1971, 1984, 2496
MboI	GATC	9 cut(s)	391, 681, 1375, 1702, 1801, 1877, 1969, 1982, 2494
MfeI	CAATTG	1 cut(s)	1938
MflI	RGATCY	5 cut(s)	1375, 1702, 1801, 1982, 2494
MhlI	GDGCHC	3 cut(s)	747, 1122, 1246
MlsI	TGGCCA	1 cut(s)	92
MluNI	TGGCCA	1 cut(s)	92
MlyI	GAGTC	3 cut(s)	431, 470, 2461
MmeI	TCCRAC	4 cut(s)	80, 345, 875, 2151
Mox20I	TGGCCA	1 cut(s)	92
Mph1103I	ATGCAT	1 cut(s)	1504
MroXI	GAANNNNTTC	1 cut(s)	2537
MscI	TGGCCA	1 cut(s)	92
MslI	CAYNNNNRTG	2 cut(s)	870, 2682
Msp20I	TGGCCA	1 cut(s)	92
MspA1I	CMGCKG	1 cut(s)	2230
MspCI	CTTAAG	1 cut(s)	1717
MspI	CCGG	5 cut(s)	61, 425, 441, 1159, 1519
MspR9I	CCNGG	8 cut(s)	61, 655, 831, 857, 1015, 1248, 1337, 1508
MunI	CAATTG	1 cut(s)	1938
MvaI	CCWGG	7 cut(s)	655, 831, 857, 1015, 1248, 1337, 1508
MvnI	CGCG	4 cut(s)	138, 270, 504, 540
MwoI	GCNNNNNNNGC	6 cut(s)	276, 1250, 1298, 1418, 1542, 2460
NciI	CCSGG	1 cut(s)	61
NcoI	CCATGG	1 cut(s)	192
NdeI	CATATG	1 cut(s)	2803
NdeII	GATC	9 cut(s)	391, 681, 1375, 1702, 1801, 1877, 1969, 1982, 2494
NlaIV	GGNNCC	6 cut(s)	379, 519, 536, 1245, 1266, 1803
NmeAIII	GCCGAG	1 cut(s)	2452
NmuCI	GTSAC	2 cut(s)	599, 2699
NruI	TCGCGA	1 cut(s)	504
NsiI	ATGCAT	1 cut(s)	1504
NspI	RCATGY	2 cut(s)	2446, 2659
OliI	CACNNNNGTG	1 cut(s)	2682
PagI	TCATGA	1 cut(s)	795
PceI	AGGCCT	2 cut(s)	2266, 2408
PciI	ACATGT	2 cut(s)	2442, 2655
PciSI	GCTCTTC	1 cut(s)	961
PcsI	WCGNNNNNNNCGW	9 cut(s)	195, 219, 231, 300, 306, 309, 312, 318, 1786
PdmI	GAANNNNTTC	1 cut(s)	2537
PflMI	CCANNNNNTGG	2 cut(s)	1114, 1688
PfoI	TCCNGGA	2 cut(s)	829, 855
PinAI	ACCGGT	1 cut(s)	1518
PkrI	GCNGC	6 cut(s)	84, 539, 574, 1651, 2462, 2800
Ple19I	CGATCG	1 cut(s)	1880
PleI	GAGTC	3 cut(s)	431, 470, 2461
PpsI	GAGTC	3 cut(s)	431, 470, 2461
PpuMI	RGGWCCY	1 cut(s)	2432
PscI	ACATGT	2 cut(s)	2442, 2655
Psp5II	RGGWCCY	1 cut(s)	2432
Psp6I	CCWGG	7 cut(s)	653, 829, 855, 1013, 1246, 1335, 1506
PspFI	CCCAGC	2 cut(s)	240, 273
PspGI	CCWGG	7 cut(s)	653, 829, 855, 1013, 1246, 1335, 1506
PspN4I	GGNNCC	6 cut(s)	379, 519, 536, 1245, 1266, 1803
PspPI	GGNCC	4 cut(s)	378, 517, 1426, 2432
PspPPI	RGGWCCY	1 cut(s)	2432
PsrI	GAACNNNNNNTAC	2 cut(s)	965, 997
PsuI	RGATCY	5 cut(s)	1375, 1702, 1801, 1982, 2494
PvuI	CGATCG	1 cut(s)	1880
PvuII	CAGCTG	1 cut(s)	2230
RgaI	GCGATCGC	1 cut(s)	1880
RruI	TCGCGA	1 cut(s)	504
RsaI	GTAC	6 cut(s)	50, 413, 2113, 2393, 2663, 2826
RsaNI	GTAC	6 cut(s)	49, 412, 2112, 2392, 2662, 2825
RseI	CAYNNNNRTG	2 cut(s)	870, 2682
SapI	GCTCTTC	1 cut(s)	961
SatI	GCNGC	6 cut(s)	83, 538, 573, 1650, 2461, 2799
Sau3AI	GATC	9 cut(s)	391, 681, 1375, 1702, 1801, 1877, 1969, 1982, 2494
Sau96I	GGNCC	4 cut(s)	378, 517, 1426, 2432
ScaI	AGTACT	1 cut(s)	2113
SchI	GAGTC	3 cut(s)	431, 470, 2461
ScrFI	CCNGG	8 cut(s)	61, 655, 831, 857, 1015, 1248, 1337, 1508
SduI	GDGCHC	3 cut(s)	747, 1122, 1246
SfaAI	GCGATCGC	1 cut(s)	1880
SfaNI	GCATC	2 cut(s)	874, 1408
SfcI	CTRYAG	2 cut(s)	355, 2115
SgfI	GCGATCGC	1 cut(s)	1880
SinI	GGWCC	3 cut(s)	378, 517, 2432
SmiI	ATTTAAAT	1 cut(s)	2016
SmiMI	CAYNNNNRTG	2 cut(s)	870, 2682
SmlI	CTYRAG	3 cut(s)	1003, 1717, 2402
SmoI	CTYRAG	3 cut(s)	1003, 1717, 2402
SseBI	AGGCCT	2 cut(s)	2266, 2408
SsiI	CCGC	9 cut(s)	262, 268, 283, 538, 701, 1448, 1462, 1612, 2629
SspI	AATATT	1 cut(s)	1457
SspMI	CTAG	6 cut(s)	158, 1148, 1413, 2429, 2457, 2570
StuI	AGGCCT	2 cut(s)	2266, 2408
StyD4I	CCNGG	8 cut(s)	59, 653, 829, 855, 1013, 1246, 1335, 1506
StyI	CCWWGG	3 cut(s)	192, 513, 2261
SwaI	ATTTAAAT	1 cut(s)	2016
TaaI	ACNGT	8 cut(s)	1100, 1630, 1672, 1678, 2078, 2111, 2119, 2247
TaiI	ACGT	2 cut(s)	318, 987
TaqI	TCGA	3 cut(s)	249, 333, 2650
TaqII	GACCGA	1 cut(s)	395
TatI	WGTACW	5 cut(s)	48, 411, 2111, 2391, 2824
TauI	GCSGC	1 cut(s)	540
TscAI	CASTG	6 cut(s)	1105, 1266, 1318, 1593, 1683, 1781
TseFI	GTSAC	2 cut(s)	599, 2699
TseI	GCWGC	5 cut(s)	82, 572, 1649, 2460, 2798
Tsp45I	GTSAC	2 cut(s)	599, 2699
TspGWI	ACGGA	2 cut(s)	14, 91
TspRI	CASTG	6 cut(s)	1105, 1266, 1318, 1593, 1683, 1781
Van91I	CCANNNNNTGG	2 cut(s)	1114, 1688
Vha464I	CTTAAG	1 cut(s)	1717
VneI	GTGCAC	1 cut(s)	1118
VpaK11BI	GGWCC	3 cut(s)	378, 517, 2432
XapI	RAATTY	3 cut(s)	664, 2000, 2186
XceI	RCATGY	2 cut(s)	2446, 2659
XcmI	CCANNNNNNNNNTGG	2 cut(s)	759, 2599
XmiI	GTMKAC	1 cut(s)	342
XmnI	GAANNNNTTC	1 cut(s)	2537
XspI	CTAG	6 cut(s)	158, 1148, 1413, 2429, 2457, 2570
ZrmI	AGTACT	1 cut(s)	2113
Zsp2I	ATGCAT	1 cut(s)	1504

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.