MD12G1077200.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003524
Physical Location & Seq
Reverse (-)
9386247 .. 9409644
23398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1077200.v1.1.491

Sequence Viewer

Length: 3114 bp
ATGAGCGCTGTTTCGGGGGTGATTTCGCGCCAAGTTTTGCCTGCCTGTGGCAGCCTTTGCTTCTTCTGCCCAGCATTGCGAGCCAGGTCTAGACAGCCGGTTAAGAGATACAAGAAGCTCATTGCTGACATTTTCCCTCGTAACCAGGAAGAAGGACCAAATGATCGGAAGATTGGAAAGTTGTGCGAATATGTTGCCAAGAATCCTTTGCGAATCCCAAAGATCACAACTTATCTAGAGCAAAGGTGCTACAAGGAATTGAGGAACGAGAACTTTCGATCTGCAAAAATTGTCATGTGCATTTATAACAAATTGCTGATTTCTTGTAAGGAGCAAATGCCTCTTTTTGCGAGTAGCTTACTCAGCATTATGCATACACTGTTGGATCAAACACGGCAAGATGAAATGCAAATTATAGGATGTCAGACTCTTTTTAACTTTGTAAATAATCAGACGGATGGAACTTATATGTTCAACTTAGAAGGCTTTATTCCAAAATTGTGTCAGATAGCTCAAGAGCCGGGGGAAGATGAAAGGGCAAGTAATCTACGTTCAGCTGCACTCCAAGCCCTTGCTTCAATGGTTTGGTTTATGGGTGAGAACTCTCATATATCAGTCGAGTTCGATAACATTGTTGTAGTTGTCTTGGAAAATTATGGTGGCTTAAATAAAACTTCAGAGAACCTTGAAGGTTCTAAGAATCGGTGGGTGCAGGAACTGCAAAGTAATGAGGGTCATGTTTCTTCTTCCCCAGATGTCGTCATCAGGCTTACATCTTGGAGCACCCTAGTGGATGACAAAGGCAATCTAAATGTGACAGTGGAAGATGCCAAGAACCCCTGCTTTTGGTCCAGGGTTTGCCTGCACAACATGGCCAAGCTAGCCAAGGAGGCCACAACAATAAGGCGTGTCCTGGAATCTTTGTTCCGTTACTTCGACACTGGGAACTTATGGTCTCCCAAATATGGTCTTGCTATCCCAGTCCTCAAGGATATACAAGTTTTAATGGATGATTGTGGGCAAAGTACACATGTTTTGCTTTCCATCTTAATCAAGCATCTGGATCACAAAAATGTCCTCAAACAACCCAACATGCAGGTCGACATAGTGGAGGTCACCGCCTCTCTTTCTCAACTTGCCAAGGTTGAGCCCTCTGTGGCAATAATTGGTGCAGTAAGTGATGCCATGAGGCATTTGAGGAAGAGCATTCACTGCTCGCTTGATGATGCTAATCTAGGAACAGATGTAATAAAATGGAACAGAAGCTTTAGAGAAGCGGTGGATAAGTGCCTTGTACAGTTATCATATAAGGTTGGAGAACCAGGACCAATCCTTGATGCAATGGCTGTTATGTTGGAGAATATCTCAACCATAACAGTTATAGCCAGAACTACAATTTCTGCTGTTTATCGTACTGCTCAAATTGTAGCTTCTTTGCCAAATTTGTCCTATCAAAACAAGGCTTTTCCTGAGGCATTGATTCATCAACTACTCCCAGCTATGGTCCATCCTGACCATGAAACACGTGTTGGAGCCCATCGCATCTTTTCCGTTGTTCTTGTTCCATCTTCAGTTTGCCCTAGTCGCTCCTTACCTAATGCTGAGTCAAAAAAGGCATTGAATTTTCCCAGGACACTCTCAAGAACTGTATCTGTCTTTTCTTCTTCAGCTGCCCTATTTGAGAAGCTAAAGAGAGAGAAAATTTCTTCACGAGATAGTATCTGTGAAGAGGACAATGAAAATTCTGTTGCTGATGGGGAACAAGGAGACACCAATAGTGGGATTCTGAGCAGATTGAGGTCTTCTTATAGTCGGACGTATAGCATGAAAAGTTCTCCTGCACCTTCAGCAATAAATGAAAATTCCGTCAACAATTCGATTAAGGAACCTCAGGAAGCTGATTCCCTCAGATTGAGCAACCACCAGATAACTCTCTTGCTTTTATCAATTTGGGTGCAATCCATCTCTCCTGGGAACATGCCTGAAAACTATGAAGCAATTGCTCACACACATAGCCTCGTGGTACTTTTTTCTTGGGCCAAGAATTCCAGAATTGAGGTTCTTGTTCGAAGTTTTCAGCTAGCATTTTCCTTGCGGAACATATCTCTTAATGAAGGAGGTGTGCTGCCACCATCGCGTCGCAGGTCCCTCTTTACCCTGGCAACTTCAATGATTCTCTTTCTATCAAAAGCATACAATATTGTTTCTCTTGTTCGTAGAGCCAATGCAGTACTTGTGGATAAAATAGTTGATCCATTCCTGCGTTTGGTTGAAGATCGCAAGTTACAGGCTGTTAAGACTGGACCGGACCATCCCAGACATCTTTATGGATCTGAAGAAGATGATCGTCTGGCATTAAAATCTCTGTCAGAGATAAATATTACTGAAGAGCAAACTAAGGAATTCTTTGCTTCTGAAGTTGTGAAGAGCTTGGAAAGATTGTTAGATGCCGAATTGTCTACTATAAGGGAGGAGCTACTCAGTGAATTCTTACCTGATGATGTATGTCCACTGGGAGCCCAGTTGTGTATGGATGCGCCGCAAAAGTTATATCAGGTTGATTCCCGAGACAGTAAATCTATGAAGGAGGATGCTCCAATATTTTCATTGGATGATGATTCCTTTCCAGGTTCATTTGATAGCCAAAAGAACAACTTAGATTTTTCTGCAGATTCTCCCAATCTTCTGAGCGTCAATCAATTTATTGAATCTGTTCTGGAGACAGCACATCAAGTTGGAAGACTGTCGGTCTCTAATGCACCTGATGTGCCATACAAGGAAATGGCCGGGCACTGTGAGGCACTCCTGATAGGAAAGCAGCAGAAGATGTCGAATTTGATGAGCTCCCAACAGAAGCAGGAGTATTTGATGAACCAGTCTTTACAAAATCATAACGATGATGCGAAATGGATAACATCTGGTTTCCATGCTGACTTCGGCTCATATAGGAGCGGAAATCCATTTGCTGATGACACTGCCACTTCATACAATCCTCCCCCAACCGCAGGCCATGCCTCAGTGATGTGTGTAAACGAATACCAGCATCATCCCCACTCTTTCAGACTACCGGCGTCGAGTCCATATGATAACTTCCTAAAAGCTGCAGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1038

Amino Acids

115.8

Weight (kDa)

6.32

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 69 - 158 2e-19 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 306
Acc36I ACCTGC 3 cut(s) 1087, 2133, 3095
AccBSI CCGCTC 1 cut(s) 2952
AccI GTMKAC 2 cut(s) 1101, 2459
AccII CGCG 2 cut(s) 28, 2137
AciI CCGC 6 cut(s) 1119, 1277, 2095, 2540, 2952, 3003
AclWI GGATC 4 cut(s) 393, 1071, 2246, 2338
AcoI YGGCCR 2 cut(s) 873, 2784
AcsI RAATTY 9 cut(s) 1441, 1621, 1701, 1741, 1861, 2044, 2402, 2486, 2833
AcuI CTGAAG 7 cut(s) 660, 1554, 1650, 1830, 2355, 2406, 2436
AcvI CACGTG 1 cut(s) 1526
AcyI GRCGYC 1 cut(s) 3071
AfaI GTAC 5 cut(s) 1027, 1296, 1414, 2025, 2232
AfeI AGCGCT 1 cut(s) 7
AfiI CCNNNNNNNGG 7 cut(s) 47, 846, 965, 1501, 1779, 2266, 3005
AflIII ACRYGT 3 cut(s) 1030, 1523, 1525
AgsI TTSAA 7 cut(s) 475, 579, 689, 1621, 2169, 2273, 2708
AhdI GACNNNNNGTC 1 cut(s) 2747
AjnI CCWGG 9 cut(s) 83, 144, 851, 912, 1321, 1628, 1969, 2157, 2626
AjuI GAANNNNNNNTTGG 2 cut(s) 1512, 1544
AleI CACNNNNGTG 1 cut(s) 788
AloI GAACNNNNNNTCC 2 cut(s) 908, 940
Alw21I GWGCWC 2 cut(s) 785, 2846
Alw26I GTCTC 5 cut(s) 960, 1761, 2562, 2714, 2755
AlwI GGATC 4 cut(s) 393, 1071, 2246, 2338
AlwNI CAGNNNCTG 1 cut(s) 718
Ama87I CYCGRG 1 cut(s) 2565
Aor51HI AGCGCT 1 cut(s) 7
AoxI GGCC 5 cut(s) 873, 891, 2037, 2784, 3007
ApeKI GCWGC 6 cut(s) 51, 557, 1670, 2125, 2818, 3101
ApoI RAATTY 9 cut(s) 1441, 1621, 1701, 1741, 1861, 2044, 2402, 2486, 2833
Asp700I GAANNNNTTC 1 cut(s) 2712
AspLEI GCGC 3 cut(s) 8, 30, 2539
AspS9I GGNCC 8 cut(s) 155, 849, 1325, 1504, 2037, 2145, 2303, 2308
AsuC2I CCSGG 2 cut(s) 522, 2788
AsuHPI GGTGA 3 cut(s) 31, 608, 1108
AsuII TTCGAA 1 cut(s) 2068
AsuNHI GCTAGC 2 cut(s) 880, 2080
AvaI CYCGRG 1 cut(s) 2565
AvaII GGWCC 7 cut(s) 155, 849, 1325, 1504, 2145, 2303, 2308
AxyI CCTNAGG 2 cut(s) 1470, 1890
BaeGI GKGCMC 1 cut(s) 2793
BalI TGGCCA 1 cut(s) 875
BanII GRGCYC 4 cut(s) 1152, 1537, 2521, 2846
BauI CACGAG 2 cut(s) 1710, 2018
BbrPI CACGTG 1 cut(s) 1526
BbsI GAAGAC 2 cut(s) 1794, 2746
Bbv12I GWGCWC 2 cut(s) 785, 2846
BbvI GCAGC 6 cut(s) 63, 544, 1657, 2112, 2830, 3088
BccI CCATC 9 cut(s) 452, 1052, 1515, 1545, 1573, 1748, 1970, 2140, 2319
BceAI ACGGC 1 cut(s) 410
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BciT130I CCWGG 9 cut(s) 85, 146, 853, 914, 1323, 1630, 1971, 2159, 2628
BcnI CCSGG 2 cut(s) 522, 2788
BcoDI GTCTC 5 cut(s) 960, 1761, 2562, 2714, 2755
BfaI CTAG 7 cut(s) 90, 236, 788, 881, 1235, 1581, 2081
BfmI CTRYAG 2 cut(s) 2667, 3102
BfoI RGCGCY 1 cut(s) 9
BfuAI ACCTGC 3 cut(s) 1087, 2133, 3095
BglI GCCNNNNNGGC 1 cut(s) 890
BisI GCNGC 7 cut(s) 52, 558, 1671, 2126, 2540, 2819, 3102
BlsI GCNGC 7 cut(s) 53, 559, 1672, 2127, 2541, 2820, 3103
BmcAI AGTACT 1 cut(s) 2232
Bme18I GGWCC 7 cut(s) 155, 849, 1325, 1504, 2145, 2303, 2308
BmeRI GACNNNNNGTC 1 cut(s) 2747
BmeT110I CYCGRG 1 cut(s) 2565
BmgT120I GGNCC 8 cut(s) 155, 849, 1325, 1504, 2037, 2145, 2303, 2308
BmiI GGNNCC 4 cut(s) 1534, 1887, 2147, 2518
BmrI ACTGGG 4 cut(s) 951, 974, 2515, 2522
BmtI GCTAGC 2 cut(s) 884, 2084
BmuI ACTGGG 4 cut(s) 951, 974, 2515, 2522
BpiI GAAGAC 2 cut(s) 1794, 2746
BplI GAGNNNNNCTC 2 cut(s) 1349, 1381
BpmI CTGGAG 1 cut(s) 2738
Bpu14I TTCGAA 1 cut(s) 2068
BpuEI CTTGAG 3 cut(s) 498, 971, 1624
BpuMI CCSGG 2 cut(s) 522, 2788
BsaAI YACGTR 1 cut(s) 1526
BsaBI GATNNNNATC 1 cut(s) 1230
BsaHI GRCGYC 1 cut(s) 3071
BsaI GGTCTC 2 cut(s) 960, 2755
BsaJI CCNNGG 7 cut(s) 521, 852, 885, 1140, 1628, 1970, 2157
BsaWI WCCGGW 1 cut(s) 2305
Bsc4I CCNNNNNNNGG 7 cut(s) 47, 846, 965, 1501, 1779, 2266, 3005
Bse118I RCCGGY 2 cut(s) 97, 3067
Bse1I ACTGG 6 cut(s) 946, 980, 2305, 2517, 2521, 2875
Bse21I CCTNAGG 2 cut(s) 1470, 1890
Bse3DI GCAATG 3 cut(s) 74, 120, 1347
Bse8I GATNNNNATC 1 cut(s) 1230
BseBI CCWGG 9 cut(s) 85, 146, 853, 914, 1323, 1630, 1971, 2159, 2628
BseDI CCNNGG 7 cut(s) 521, 852, 885, 1140, 1628, 1970, 2157
BseJI GATNNNNATC 1 cut(s) 1230
BseLI CCNNNNNNNGG 7 cut(s) 47, 846, 965, 1501, 1779, 2266, 3005
BseMI GCAATG 3 cut(s) 74, 120, 1347
BseMII CTCAG 9 cut(s) 376, 1461, 1593, 1778, 1904, 1921, 2494, 2678, 3030
BseNI ACTGG 6 cut(s) 946, 980, 2305, 2517, 2521, 2875
BseRI GAGGAG 1 cut(s) 2486
BseSI GKGCMC 1 cut(s) 2793
BseXI GCAGC 6 cut(s) 63, 544, 1657, 2112, 2830, 3088
BseYI CCCAGC 2 cut(s) 70, 1495
BsgI GTGCAG 5 cut(s) 543, 731, 848, 1191, 1824
Bsh1236I CGCG 2 cut(s) 28, 2137
BshFI GGCC 5 cut(s) 875, 893, 2039, 2786, 3009
BsiHKAI GWGCWC 2 cut(s) 785, 2846
BsiHKCI CYCGRG 1 cut(s) 2565
BsiSI CCGG 5 cut(s) 98, 521, 2306, 2787, 3068
BslFI GGGAC 1 cut(s) 2131
BslI CCNNNNNNNGG 7 cut(s) 47, 846, 965, 1501, 1779, 2266, 3005
BsmAI GTCTC 5 cut(s) 960, 1761, 2562, 2714, 2755
BsmFI GGGAC 1 cut(s) 2131
BsmI GAATGC 1 cut(s) 1206
BsnI GGCC 5 cut(s) 875, 893, 2039, 2786, 3009
Bso31I GGTCTC 2 cut(s) 960, 2755
BsoBI CYCGRG 1 cut(s) 2565
Bsp119I TTCGAA 1 cut(s) 2068
Bsp1286I GDGCHC 6 cut(s) 785, 1152, 1537, 2521, 2793, 2846
Bsp1407I TGTACA 1 cut(s) 1294
Bsp143I GATC 9 cut(s) 163, 222, 278, 385, 1063, 2251, 2275, 2330, 2344
BspACI CCGC 6 cut(s) 1119, 1277, 2095, 2540, 2952, 3003
BspANI GGCC 5 cut(s) 875, 893, 2039, 2786, 3009
BspCNI CTCAG 9 cut(s) 375, 1462, 1594, 1779, 1903, 1920, 2493, 2679, 3029
BspFNI CGCG 2 cut(s) 28, 2137
BspLI GGNNCC 4 cut(s) 1534, 1887, 2147, 2518
BspMAI CTGCAG 2 cut(s) 2671, 3106
BspMI ACCTGC 3 cut(s) 1087, 2133, 3095
BspOI GCTAGC 2 cut(s) 884, 2084
BspPI GGATC 4 cut(s) 393, 1071, 2246, 2338
BspQI GCTCTTC 3 cut(s) 1196, 2382, 2420
BspT104I TTCGAA 1 cut(s) 2068
BspTNI GGTCTC 2 cut(s) 960, 2755
BsrBI CCGCTC 1 cut(s) 2952
BsrDI GCAATG 3 cut(s) 74, 120, 1347
BsrFI RCCGGY 2 cut(s) 97, 3067
BsrGI TGTACA 1 cut(s) 1294
BsrI ACTGG 6 cut(s) 946, 980, 2305, 2517, 2521, 2875
BssAI RCCGGY 2 cut(s) 97, 3067
BssECI CCNNGG 7 cut(s) 521, 852, 885, 1140, 1628, 1970, 2157
BssMI GATC 9 cut(s) 163, 222, 278, 385, 1063, 2251, 2275, 2330, 2344
BssNI GRCGYC 1 cut(s) 3071
BssSI CACGAG 2 cut(s) 1710, 2018
BssT1I CCWWGG 2 cut(s) 885, 1140
Bst2BI CACGAG 2 cut(s) 1710, 2018
Bst2UI CCWGG 9 cut(s) 85, 146, 853, 914, 1323, 1630, 1971, 2159, 2628
Bst4CI ACNGT 8 cut(s) 381, 820, 1299, 1378, 1648, 2573, 2745, 2795
Bst6I CTCTTC 4 cut(s) 1196, 1722, 2382, 2420
BstACI GRCGYC 1 cut(s) 3071
BstAPI GCANNNNNTGC 4 cut(s) 57, 718, 1212, 3011
BstAUI TGTACA 1 cut(s) 1294
BstBAI YACGTR 1 cut(s) 1526
BstBI TTCGAA 1 cut(s) 2068
BstC8I GCNNGC 7 cut(s) 42, 81, 863, 882, 1217, 2082, 3007
BstEII GGTNACC 1 cut(s) 1114
BstFNI CGCG 2 cut(s) 28, 2137
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 3 cut(s) 8, 30, 2539
BstKTI GATC 9 cut(s) 166, 225, 281, 388, 1066, 2254, 2278, 2333, 2347
BstMAI GTCTC 5 cut(s) 960, 1761, 2562, 2714, 2755
BstMBI GATC 9 cut(s) 163, 222, 278, 385, 1063, 2251, 2275, 2330, 2344
BstNI CCWGG 9 cut(s) 85, 146, 853, 914, 1323, 1630, 1971, 2159, 2628
BstNSI RCATGY 3 cut(s) 1034, 1096, 1981
BstPI GGTNACC 1 cut(s) 1114
BstSFI CTRYAG 2 cut(s) 2667, 3102
BstSLI GKGCMC 1 cut(s) 2793
BstUI CGCG 2 cut(s) 28, 2137
BstV1I GCAGC 6 cut(s) 63, 544, 1657, 2112, 2830, 3088
BstV2I GAAGAC 2 cut(s) 1794, 2746
BstX2I RGATCY 1 cut(s) 2330
BstYI RGATCY 1 cut(s) 2330
Bsu36I CCTNAGG 2 cut(s) 1470, 1890
BsuRI GGCC 5 cut(s) 875, 893, 2039, 2786, 3009
BtgZI GCGATG 2 cut(s) 1523, 2118
BtsI GCAGTG 2 cut(s) 1210, 2973
BtsIMutI CAGTG 9 cut(s) 377, 825, 939, 1210, 2488, 2510, 2791, 2973, 3024
BveI ACCTGC 3 cut(s) 1087, 2133, 3095
Cac8I GCNNGC 7 cut(s) 42, 81, 863, 882, 1217, 2082, 3007
CaiI CAGNNNCTG 1 cut(s) 718
CfoI GCGC 3 cut(s) 8, 30, 2539
Cfr10I RCCGGY 2 cut(s) 97, 3067
Cfr13I GGNCC 8 cut(s) 155, 849, 1325, 1504, 2037, 2145, 2303, 2308
CseI GACGC 3 cut(s) 2126, 2680, 3060
Csp6I GTAC 5 cut(s) 1026, 1295, 1413, 2024, 2231
CviQI GTAC 5 cut(s) 1026, 1295, 1413, 2024, 2231
DpnI GATC 9 cut(s) 165, 224, 280, 387, 1065, 2253, 2277, 2332, 2346
DpnII GATC 9 cut(s) 163, 222, 278, 385, 1063, 2251, 2275, 2330, 2344
DriI GACNNNNNGTC 1 cut(s) 2747
EaeI YGGCCR 2 cut(s) 873, 2784
Eam1104I CTCTTC 4 cut(s) 1196, 1722, 2382, 2420
Eam1105I GACNNNNNGTC 1 cut(s) 2747
EarI CTCTTC 4 cut(s) 1196, 1722, 2382, 2420
Ecl136II GAGCTC 1 cut(s) 2844
Eco130I CCWWGG 2 cut(s) 885, 1140
Eco24I GRGCYC 4 cut(s) 1152, 1537, 2521, 2846
Eco31I GGTCTC 2 cut(s) 960, 2755
Eco47I GGWCC 7 cut(s) 155, 849, 1325, 1504, 2145, 2303, 2308
Eco47III AGCGCT 1 cut(s) 7
Eco53kI GAGCTC 1 cut(s) 2844
Eco57I CTGAAG 7 cut(s) 660, 1554, 1650, 1830, 2355, 2406, 2436
Eco72I CACGTG 1 cut(s) 1526
Eco81I CCTNAGG 2 cut(s) 1470, 1890
Eco88I CYCGRG 1 cut(s) 2565
Eco91I GGTNACC 1 cut(s) 1114
EcoICRI GAGCTC 1 cut(s) 2844
EcoO109I RGGNCCY 1 cut(s) 2145
EcoO65I GGTNACC 1 cut(s) 1114
EcoRI GAATTC 3 cut(s) 2044, 2402, 2486
EcoRII CCWGG 9 cut(s) 83, 144, 851, 912, 1321, 1628, 1969, 2157, 2626
EcoT14I CCWWGG 2 cut(s) 885, 1140
EcoT22I ATGCAT 1 cut(s) 375
EcoT38I GRGCYC 4 cut(s) 1152, 1537, 2521, 2846
ErhI CCWWGG 2 cut(s) 885, 1140
FalI AAGNNNNNCTT 4 cut(s) 2390, 2422, 2639, 2671
FaqI GGGAC 1 cut(s) 2131
FauNDI CATATG 1 cut(s) 3082
FblI GTMKAC 2 cut(s) 1101, 2459
Fnu4HI GCNGC 7 cut(s) 52, 558, 1671, 2126, 2540, 2819, 3102
FriOI GRGCYC 4 cut(s) 1152, 1537, 2521, 2846
Fsp4HI GCNGC 7 cut(s) 52, 558, 1671, 2126, 2540, 2819, 3102
FspBI CTAG 7 cut(s) 90, 236, 788, 881, 1235, 1581, 2081
GlaI GCGC 3 cut(s) 7, 29, 2538
GluI GCNGC 7 cut(s) 52, 558, 1671, 2126, 2540, 2819, 3102
GsaI CCCAGC 2 cut(s) 74, 1499
GsuI CTGGAG 1 cut(s) 2738
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 5 cut(s) 875, 893, 2039, 2786, 3009
HapII CCGG 5 cut(s) 98, 521, 2306, 2787, 3068
HgaI GACGC 3 cut(s) 2126, 2680, 3060
HhaI GCGC 3 cut(s) 8, 30, 2539
Hin1I GRCGYC 1 cut(s) 3071
Hin6I GCGC 3 cut(s) 6, 28, 2537
HinP1I GCGC 3 cut(s) 6, 28, 2537
HincII GTYRAC 2 cut(s) 1102, 1870
HindII GTYRAC 2 cut(s) 1102, 1870
HindIII AAGCTT 1 cut(s) 1264
HpaII CCGG 5 cut(s) 98, 521, 2306, 2787, 3068
HphI GGTGA 3 cut(s) 31, 608, 1108
Hpy166II GTNNAC 6 cut(s) 1028, 1102, 1870, 2460, 2510, 3031
Hpy8I GTNNAC 6 cut(s) 1028, 1102, 1870, 2460, 2510, 3031
Hpy99I CGWCG 2 cut(s) 2142, 3076
HpyAV CCTTC 6 cut(s) 146, 476, 683, 1854, 2108, 2578
HpyCH4III ACNGT 8 cut(s) 381, 820, 1299, 1378, 1648, 2573, 2745, 2795
HpyCH4IV ACGT 3 cut(s) 550, 1525, 1817
HpySE526I ACGT 3 cut(s) 550, 1525, 1817
Hsp92I GRCGYC 1 cut(s) 3071
HspAI GCGC 3 cut(s) 6, 28, 2537
Kzo9I GATC 9 cut(s) 163, 222, 278, 385, 1063, 2251, 2275, 2330, 2344
LguI GCTCTTC 3 cut(s) 1196, 2382, 2420
LmnI GCTCC 9 cut(s) 331, 780, 1532, 1592, 2473, 2516, 2599, 2849, 2949
Lsp1109I GCAGC 6 cut(s) 63, 544, 1657, 2112, 2830, 3088
MaeI CTAG 7 cut(s) 90, 236, 788, 881, 1235, 1581, 2081
MaeII ACGT 3 cut(s) 550, 1525, 1817
MaeIII GTNAC 5 cut(s) 140, 814, 929, 1114, 2283
MalI GATC 9 cut(s) 165, 224, 280, 387, 1065, 2253, 2277, 2332, 2346
MbiI CCGCTC 1 cut(s) 2952
MboI GATC 9 cut(s) 163, 222, 278, 385, 1063, 2251, 2275, 2330, 2344
MfeI CAATTG 1 cut(s) 1998
MflI RGATCY 1 cut(s) 2330
MhlI GDGCHC 6 cut(s) 785, 1152, 1537, 2521, 2793, 2846
MlsI TGGCCA 1 cut(s) 875
MluNI TGGCCA 1 cut(s) 875
MlyI GAGTC 3 cut(s) 421, 1613, 3085
MmeI TCCRAC 6 cut(s) 363, 1294, 1335, 1510, 1793, 2716
Mox20I TGGCCA 1 cut(s) 875
Mph1103I ATGCAT 1 cut(s) 375
MroXI GAANNNNTTC 1 cut(s) 2712
MscI TGGCCA 1 cut(s) 875
MseI TTAA 9 cut(s) 102, 435, 665, 1004, 1049, 1881, 2109, 2295, 2357
MslI CAYNNNNRTG 4 cut(s) 788, 1071, 2325, 2895
Msp20I TGGCCA 1 cut(s) 875
MspA1I CMGCKG 2 cut(s) 557, 1670
MspI CCGG 5 cut(s) 98, 521, 2306, 2787, 3068
MunI CAATTG 1 cut(s) 1998
Mva1269I GAATGC 1 cut(s) 1206
MvaI CCWGG 9 cut(s) 85, 146, 853, 914, 1323, 1630, 1971, 2159, 2628
MvnI CGCG 2 cut(s) 28, 2137
NciI CCSGG 2 cut(s) 522, 2788
NdeI CATATG 1 cut(s) 3082
NdeII GATC 9 cut(s) 163, 222, 278, 385, 1063, 2251, 2275, 2330, 2344
NheI GCTAGC 2 cut(s) 880, 2080
NlaIV GGNNCC 4 cut(s) 1534, 1887, 2147, 2518
NmuCI GTSAC 2 cut(s) 814, 1114
NsiI ATGCAT 1 cut(s) 375
NspI RCATGY 3 cut(s) 1034, 1096, 1981
NspV TTCGAA 1 cut(s) 2068
OliI CACNNNNGTG 1 cut(s) 788
PciI ACATGT 1 cut(s) 1030
PciSI GCTCTTC 3 cut(s) 1196, 2382, 2420
PctI GAATGC 1 cut(s) 1206
PdmI GAANNNNTTC 1 cut(s) 2712
PfoI TCCNGGA 1 cut(s) 912
PkrI GCNGC 7 cut(s) 53, 559, 1672, 2127, 2541, 2820, 3103
PleI GAGTC 3 cut(s) 421, 1612, 3084
PmaCI CACGTG 1 cut(s) 1526
PmlI CACGTG 1 cut(s) 1526
PpsI GAGTC 3 cut(s) 421, 1612, 3084
Ppu21I YACGTR 1 cut(s) 1526
PpuMI RGGWCCY 1 cut(s) 2145
PscI ACATGT 1 cut(s) 1030
PsiI TTATAA 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 2846
Psp5II RGGWCCY 1 cut(s) 2145
Psp6I CCWGG 9 cut(s) 83, 144, 851, 912, 1321, 1628, 1969, 2157, 2626
PspCI CACGTG 1 cut(s) 1526
PspEI GGTNACC 1 cut(s) 1114
PspFI CCCAGC 2 cut(s) 70, 1495
PspGI CCWGG 9 cut(s) 83, 144, 851, 912, 1321, 1628, 1969, 2157, 2626
PspN4I GGNNCC 4 cut(s) 1534, 1887, 2147, 2518
PspPI GGNCC 8 cut(s) 155, 849, 1325, 1504, 2037, 2145, 2303, 2308
PspPPI RGGWCCY 1 cut(s) 2145
PsrI GAACNNNNNNTAC 2 cut(s) 535, 567
PstI CTGCAG 2 cut(s) 2671, 3106
PstNI CAGNNNCTG 1 cut(s) 718
PsuI RGATCY 1 cut(s) 2330
PvuII CAGCTG 2 cut(s) 557, 1670
RsaI GTAC 5 cut(s) 1027, 1296, 1414, 2025, 2232
RsaNI GTAC 5 cut(s) 1026, 1295, 1413, 2024, 2231
RseI CAYNNNNRTG 4 cut(s) 788, 1071, 2325, 2895
SacI GAGCTC 1 cut(s) 2846
SalI GTCGAC 1 cut(s) 1100
SapI GCTCTTC 3 cut(s) 1196, 2382, 2420
SaqAI TTAA 9 cut(s) 102, 435, 665, 1004, 1049, 1881, 2109, 2295, 2357
SatI GCNGC 7 cut(s) 52, 558, 1671, 2126, 2540, 2819, 3102
Sau3AI GATC 9 cut(s) 163, 222, 278, 385, 1063, 2251, 2275, 2330, 2344
Sau96I GGNCC 8 cut(s) 155, 849, 1325, 1504, 2037, 2145, 2303, 2308
ScaI AGTACT 1 cut(s) 2232
SchI GAGTC 3 cut(s) 421, 1613, 3085
SduI GDGCHC 6 cut(s) 785, 1152, 1537, 2521, 2793, 2846
SfcI CTRYAG 2 cut(s) 2667, 3102
SfuI TTCGAA 1 cut(s) 2068
SinI GGWCC 7 cut(s) 155, 849, 1325, 1504, 2145, 2303, 2308
SmiMI CAYNNNNRTG 4 cut(s) 788, 1071, 2325, 2895
SmlI CTYRAG 3 cut(s) 513, 986, 1639
SmoI CTYRAG 3 cut(s) 513, 986, 1639
SsiI CCGC 6 cut(s) 1119, 1277, 2095, 2540, 2952, 3003
SspI AATATT 3 cut(s) 2200, 2380, 2601
SspMI CTAG 7 cut(s) 90, 236, 788, 881, 1235, 1581, 2081
SstI GAGCTC 1 cut(s) 2846
StyI CCWWGG 2 cut(s) 885, 1140
TaaI ACNGT 8 cut(s) 381, 820, 1299, 1378, 1648, 2573, 2745, 2795
TaiI ACGT 3 cut(s) 553, 1528, 1820
TaqI TCGA 9 cut(s) 277, 618, 624, 936, 1101, 1877, 2068, 2831, 3074
TaqII GACCGA 1 cut(s) 2737
TatI WGTACW 3 cut(s) 1025, 1294, 2230
TauI GCSGC 1 cut(s) 2542
Tru1I TTAA 9 cut(s) 102, 435, 665, 1004, 1049, 1881, 2109, 2295, 2357
Tru9I TTAA 9 cut(s) 102, 435, 665, 1004, 1049, 1881, 2109, 2295, 2357
TscAI CASTG 9 cut(s) 384, 825, 946, 1217, 2488, 2517, 2798, 2980, 3024
TseFI GTSAC 2 cut(s) 814, 1114
TseI GCWGC 6 cut(s) 51, 557, 1670, 2125, 2818, 3101
Tsp45I GTSAC 2 cut(s) 814, 1114
TspGWI ACGGA 4 cut(s) 470, 917, 1540, 1855
TspRI CASTG 9 cut(s) 384, 825, 946, 1217, 2488, 2517, 2798, 2980, 3024
VpaK11BI GGWCC 7 cut(s) 155, 849, 1325, 1504, 2145, 2303, 2308
XapI RAATTY 9 cut(s) 1441, 1621, 1701, 1741, 1861, 2044, 2402, 2486, 2833
XbaI TCTAGA 2 cut(s) 89, 235
XceI RCATGY 3 cut(s) 1034, 1096, 1981
XmiI GTMKAC 2 cut(s) 1101, 2459
XmnI GAANNNNTTC 1 cut(s) 2712
XspI CTAG 7 cut(s) 90, 236, 788, 881, 1235, 1581, 2081
ZrmI AGTACT 1 cut(s) 2232
Zsp2I ATGCAT 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.