Rw0G004350

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00166
OG0003524
Physical Location & Seq
Forward (+)
32373 .. 36945
4573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G004350.1

Sequence Viewer

Length: 1680 bp
ATGCAAGGGGGAGTCCTCAGAGACCATTTTATCACAACCTCTCTTGAGCAAAGGTGTTACAAAGAATTGAGAAATGAGAATTTTCGATCCACAAAAATTGTCATGTGCATTTACCAGAAATTGCTGATAACTTGCAAGGAGCAAATGCCTCTCTTTGCGAGTAGCTTGCTCAGCATTATGCATACCCTCCTGGATCAATCACGCCAAGATGAAATGCAAATTATAGGATGCCAGACTCTTTTTGACTTTGTAAATAATCAGGTGGATGGAACATACATGTTTAACTTAGAAGGATTTATTCCGAAACTCTGTCAGATAGCTCAAGAGCCAGGGGAAGATGAAAGGGCAAATAATTTACGTTGTGCTGCACTGCAAGCCCTTTCTTCAATGGTTTGGTTTATGGGCGAGAATTCTCACATATCAGTCGAGTTCGATAATATTGTTGCAGTGGTCTTGGAAAACTATGCAGGTTTTAAGGAAGCTGAAGGTTCGCAGATTCAGTGGGTGCAGGAAGTTCAAAAAGATGAGGGTCATGTTTCTCCACAAGATGTCAGAATCAGGATTCCTCCTTGGAGTGCCATTGTGGATGCCAAAGGCGAACTAAAAGTATCAGAGGAAGATGCCAAGAACCTCTGTTTTTGGTCCAGGGTTTGCCTTCATAACATGGCAAAGTTAGCCAAAGAGGCTACAACAATAAGACGTGTCCTGGAATCTTTGTTCCGCTACTTTGATAACGAAAACTTGTGGTCTCTTGAACATGGGCTTGCCATCTCAGTCTTAAAGGATATACAATTCTTAATGGATAAATCTGGGCAAAATACCCATGTTTTGCTATCCATCTTAATCAAGCATCTCGACCACAAAAATGTCCTTAAACAACCTAACATGCAGCTTGACATAGTGGAGGTAACTACCTCCCTCGCTCTACATGCCAAGGTTGAACCTTCTGTAGCAATAATTGGGGCTTTAAGTGATGCCTTGAGGCATTTGAGAAAGAGCATCCACTGCTCTCTGGATGATGCCAATCTCGGGACAGATGTCATCAAATGGAACAAATGCTTTAGAGAAGAGGTGGATAAGTGCCTTGTACAGCTATCATATAAGATCGGAGAACCAGGACCAATTCTTGATGCTATGGCTGTTATGCTGGAGAATATTTCAACTATTACAGTGATAGCCAGAACTACAATTGCTGCTATTTATCGCACTGCTCAAATTGTAGCTTCCTTACCAAATTTGTCCTATCAAAACAAGGCTTTCCCGGAGGCATTGTTTCACCAATTACTCCCAGCTATGGTTCATCCAGACCATGAAACACGTGTTGGAGCTCATCGCATCTTCTCTGTTGTTCTTGTGCCATCTTCAGTTTCGCCTAATCTATTCTCATCTAATACCGAGACAAAAAAGGCCTTTGATCTTCACAGGACACTCTCAAGAACTGTCTCTGTGTTTTCTTCTTCAGCTGCCCTTTTTGAGAAGCTGAGGAGAGAAAAAATTTCCTCACGGGAAAGTATTTGTGAAGAAGATGAAAACATTGTTCCGGAGGGGGAAGAAAGAGACACCAATAATGGGTTTCTGAGCAGATTGAAGTCATCTTATAGTCGGACATATAGTATGAAAAGCTTGCCTGCACCTTCAACACCAAATGAAAATTCCATGAGCAATAATACTAAGGAACTT
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

560

Amino Acids

63.26

Weight (kDa)

5.98

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 11 - 94 1.7e-15 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 458
AccIII TCCGGA 1 cut(s) 1540
AciI CCGC 1 cut(s) 721
AclWI GGATC 2 cut(s) 81, 201
AcsI RAATTY 5 cut(s) 79, 409, 1234, 1494, 1651
AcuI CTGAAG 3 cut(s) 504, 1347, 1443
AcvI CACGTG 1 cut(s) 1319
AfaI GTAC 1 cut(s) 1089
AfiI CCNNNNNNNGG 3 cut(s) 1029, 1294, 1569
AflIII ACRYGT 4 cut(s) 276, 700, 1316, 1318
AgsI TTSAA 7 cut(s) 387, 518, 755, 941, 1161, 1588, 1638
AjiI CACGTC 1 cut(s) 701
AjnI CCWGG 5 cut(s) 189, 328, 644, 705, 1114
AjuI GAANNNNNNNTTGG 4 cut(s) 1305, 1337, 1636, 1668
AloI GAACNNNNNNTCC 2 cut(s) 701, 733
Alw21I GWGCWC 1 cut(s) 1330
Alw26I GTCTC 5 cut(s) 15, 753, 1391, 1447, 1551
AlwI GGATC 2 cut(s) 81, 201
Ama87I CYCGRG 1 cut(s) 1028
Aor13HI TCCGGA 1 cut(s) 1540
AoxI GGCC 1 cut(s) 1407
ApeKI GCWGC 4 cut(s) 365, 889, 1193, 1463
ApoI RAATTY 5 cut(s) 79, 409, 1234, 1494, 1651
AspS9I GGNCC 2 cut(s) 642, 1118
AsuC2I CCSGG 1 cut(s) 1262
AsuHPI GGTGA 1 cut(s) 1268
AvaI CYCGRG 1 cut(s) 1028
AvaII GGWCC 2 cut(s) 642, 1118
BanII GRGCYC 1 cut(s) 1330
BbrPI CACGTG 1 cut(s) 1319
Bbv12I GWGCWC 1 cut(s) 1330
BbvCI CCTCAGC 1 cut(s) 1481
BbvI GCAGC 4 cut(s) 352, 901, 1180, 1450
BccI CCATC 4 cut(s) 260, 776, 845, 1366
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BciT130I CCWGG 5 cut(s) 191, 330, 646, 707, 1116
BcnI CCSGG 1 cut(s) 1262
BcoDI GTCTC 5 cut(s) 15, 753, 1391, 1447, 1551
BfmI CTRYAG 1 cut(s) 948
BfuAI ACCTGC 1 cut(s) 458
BglI GCCNNNNNGGC 1 cut(s) 683
BisI GCNGC 4 cut(s) 366, 890, 1194, 1464
BlpI GCTNAGC 1 cut(s) 170
BlsI GCNGC 4 cut(s) 367, 891, 1195, 1465
Bme1390I CCNGG 6 cut(s) 191, 330, 646, 707, 1116, 1262
Bme18I GGWCC 2 cut(s) 642, 1118
BmeT110I CYCGRG 1 cut(s) 1028
BmgBI CACGTC 1 cut(s) 701
BmgT120I GGNCC 2 cut(s) 642, 1118
BmrFI CCNGG 6 cut(s) 191, 330, 646, 707, 1116, 1262
BmsI GCATC 9 cut(s) 218, 577, 610, 859, 964, 1008, 1009, 1120, 1344
BoxI GACNNNNGTC 1 cut(s) 1037
BpmI CTGGAG 1 cut(s) 1169
Bpu10I CCTNAGC 1 cut(s) 1481
Bpu1102I GCTNAGC 1 cut(s) 170
BpuEI CTTGAG 4 cut(s) 65, 306, 1000, 1417
BpuMI CCSGG 1 cut(s) 1262
BsaAI YACGTR 1 cut(s) 1319
BsaBI GATNNNNATC 1 cut(s) 1023
BsaI GGTCTC 2 cut(s) 15, 753
BsaJI CCNNGG 4 cut(s) 329, 569, 645, 933
BsaWI WCCGGW 1 cut(s) 1540
BsaXI ACNNNNNCTCC 2 cut(s) 1256, 1286
Bsc4I CCNNNNNNNGG 3 cut(s) 1029, 1294, 1569
Bse8I GATNNNNATC 1 cut(s) 1023
BseAI TCCGGA 1 cut(s) 1540
BseBI CCWGG 5 cut(s) 191, 330, 646, 707, 1116
BseDI CCNNGG 4 cut(s) 329, 569, 645, 933
BseGI GGATG 6 cut(s) 233, 271, 592, 999, 1021, 1300
BseJI GATNNNNATC 1 cut(s) 1023
BseLI CCNNNNNNNGG 3 cut(s) 1029, 1294, 1569
BseMII CTCAG 5 cut(s) 31, 184, 786, 1472, 1568
BseRI GAGGAG 1 cut(s) 1498
BseXI GCAGC 4 cut(s) 352, 901, 1180, 1450
BseYI CCCAGC 1 cut(s) 1288
BsgI GTGCAG 3 cut(s) 351, 527, 1614
BshFI GGCC 1 cut(s) 1409
BsiHKAI GWGCWC 1 cut(s) 1330
BsiHKCI CYCGRG 1 cut(s) 1028
BsiSI CCGG 2 cut(s) 1262, 1541
BslFI GGGAC 1 cut(s) 1045
BslI CCNNNNNNNGG 3 cut(s) 1029, 1294, 1569
BsmAI GTCTC 5 cut(s) 15, 753, 1391, 1447, 1551
BsmFI GGGAC 1 cut(s) 1045
BsnI GGCC 1 cut(s) 1409
Bso31I GGTCTC 2 cut(s) 15, 753
BsoBI CYCGRG 1 cut(s) 1028
Bsp1286I GDGCHC 1 cut(s) 1330
Bsp13I TCCGGA 1 cut(s) 1540
Bsp1407I TGTACA 1 cut(s) 1087
Bsp143I GATC 4 cut(s) 86, 193, 1104, 1414
Bsp1720I GCTNAGC 1 cut(s) 170
BspACI CCGC 1 cut(s) 721
BspANI GGCC 1 cut(s) 1409
BspCNI CTCAG 5 cut(s) 30, 183, 785, 1473, 1569
BspEI TCCGGA 1 cut(s) 1540
BspMI ACCTGC 1 cut(s) 458
BspPI GGATC 2 cut(s) 81, 201
BspTNI GGTCTC 2 cut(s) 15, 753
BsrGI TGTACA 1 cut(s) 1087
BssECI CCNNGG 4 cut(s) 329, 569, 645, 933
BssMI GATC 4 cut(s) 86, 193, 1104, 1414
BssT1I CCWWGG 2 cut(s) 569, 933
Bst2UI CCWGG 5 cut(s) 191, 330, 646, 707, 1116
Bst4CI ACNGT 2 cut(s) 1171, 1441
Bst6I CTCTTC 1 cut(s) 1062
BstAPI GCANNNNNTGC 1 cut(s) 1005
BstAUI TGTACA 1 cut(s) 1087
BstBAI YACGTR 1 cut(s) 1319
BstC8I GCNNGC 5 cut(s) 167, 375, 765, 1625, 1629
BstDEI CTNAG 7 cut(s) 17, 170, 286, 772, 1481, 1577, 1671
BstF5I GGATG 6 cut(s) 233, 271, 592, 999, 1021, 1300
BstKTI GATC 4 cut(s) 89, 196, 1107, 1417
BstMAI GTCTC 5 cut(s) 15, 753, 1391, 1447, 1551
BstMBI GATC 4 cut(s) 86, 193, 1104, 1414
BstMWI GCNNNNNNNGC 6 cut(s) 171, 374, 674, 683, 929, 1005
BstNI CCWGG 5 cut(s) 191, 330, 646, 707, 1116
BstNSI RCATGY 3 cut(s) 280, 889, 932
BstPAI GACNNNNGTC 1 cut(s) 1037
BstSCI CCNGG 6 cut(s) 189, 328, 644, 705, 1114, 1260
BstSFI CTRYAG 1 cut(s) 948
BstV1I GCAGC 4 cut(s) 352, 901, 1180, 1450
BsuRI GGCC 1 cut(s) 1409
BtgZI GCGATG 1 cut(s) 1316
BtrI CACGTC 1 cut(s) 701
BtsCI GGATG 6 cut(s) 233, 271, 592, 999, 1021, 1300
BtsI GCAGTG 4 cut(s) 368, 453, 1003, 1206
BtsIMutI CAGTG 6 cut(s) 368, 453, 506, 1003, 1176, 1206
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 5 cut(s) 167, 375, 765, 1625, 1629
Cfr13I GGNCC 2 cut(s) 642, 1118
Csp6I GTAC 1 cut(s) 1088
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviQI GTAC 1 cut(s) 1088
DdeI CTNAG 7 cut(s) 17, 170, 286, 772, 1481, 1577, 1671
DpnI GATC 4 cut(s) 88, 195, 1106, 1416
DpnII GATC 4 cut(s) 86, 193, 1104, 1414
Eam1104I CTCTTC 1 cut(s) 1062
EarI CTCTTC 1 cut(s) 1062
Ecl136II GAGCTC 1 cut(s) 1328
Eco130I CCWWGG 2 cut(s) 569, 933
Eco147I AGGCCT 1 cut(s) 1409
Eco24I GRGCYC 1 cut(s) 1330
Eco31I GGTCTC 2 cut(s) 15, 753
Eco47I GGWCC 2 cut(s) 642, 1118
Eco53kI GAGCTC 1 cut(s) 1328
Eco57I CTGAAG 3 cut(s) 504, 1347, 1443
Eco72I CACGTG 1 cut(s) 1319
Eco88I CYCGRG 1 cut(s) 1028
EcoICRI GAGCTC 1 cut(s) 1328
EcoRI GAATTC 1 cut(s) 409
EcoRII CCWGG 5 cut(s) 189, 328, 644, 705, 1114
EcoT14I CCWWGG 2 cut(s) 569, 933
EcoT22I ATGCAT 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 1330
ErhI CCWWGG 2 cut(s) 569, 933
FaqI GGGAC 1 cut(s) 1045
Fnu4HI GCNGC 4 cut(s) 366, 890, 1194, 1464
FokI GGATG 6 cut(s) 240, 278, 599, 986, 1028, 1287
FriOI GRGCYC 1 cut(s) 1330
Fsp4HI GCNGC 4 cut(s) 366, 890, 1194, 1464
GluI GCNGC 4 cut(s) 366, 890, 1194, 1464
GsaI CCCAGC 1 cut(s) 1292
GsuI CTGGAG 1 cut(s) 1169
HaeIII GGCC 1 cut(s) 1409
HapII CCGG 2 cut(s) 1262, 1541
HindIII AAGCTT 1 cut(s) 1621
HinfI GANTC 6 cut(s) 12, 235, 496, 555, 562, 710
HpaII CCGG 2 cut(s) 1262, 1541
HphI GGTGA 1 cut(s) 1268
Hpy188I TCNGA 8 cut(s) 20, 303, 315, 554, 613, 1109, 1578, 1605
HpyAV CCTTC 5 cut(s) 284, 479, 665, 954, 1644
HpyCH4III ACNGT 2 cut(s) 1171, 1441
HpyCH4IV ACGT 3 cut(s) 358, 700, 1318
HpyF10VI GCNNNNNNNGC 6 cut(s) 171, 374, 674, 683, 929, 1005
HpyF3I CTNAG 7 cut(s) 17, 170, 286, 772, 1481, 1577, 1671
HpySE526I ACGT 3 cut(s) 358, 700, 1318
Kpn2I TCCGGA 1 cut(s) 1540
Kzo9I GATC 4 cut(s) 86, 193, 1104, 1414
LmnI GCTCC 2 cut(s) 139, 1325
Lsp1109I GCAGC 4 cut(s) 352, 901, 1180, 1450
LweI GCATC 9 cut(s) 218, 577, 610, 859, 964, 1008, 1009, 1120, 1344
MaeII ACGT 3 cut(s) 358, 700, 1318
MaeIII GTNAC 2 cut(s) 56, 907
MalI GATC 4 cut(s) 88, 195, 1106, 1416
MboI GATC 4 cut(s) 86, 193, 1104, 1414
MfeI CAATTG 1 cut(s) 1188
MhlI GDGCHC 1 cut(s) 1330
MlyI GAGTC 2 cut(s) 21, 229
MmeI TCCRAC 2 cut(s) 1303, 1583
Mph1103I ATGCAT 1 cut(s) 183
MroI TCCGGA 1 cut(s) 1540
MseI TTAA 7 cut(s) 282, 474, 779, 797, 842, 873, 968
MslI CAYNNNNRTG 1 cut(s) 864
MspA1I CMGCKG 1 cut(s) 1463
MspI CCGG 2 cut(s) 1262, 1541
MspR9I CCNGG 6 cut(s) 191, 330, 646, 707, 1116, 1262
MunI CAATTG 1 cut(s) 1188
MvaI CCWGG 5 cut(s) 191, 330, 646, 707, 1116
MwoI GCNNNNNNNGC 6 cut(s) 171, 374, 674, 683, 929, 1005
NciI CCSGG 1 cut(s) 1262
NdeII GATC 4 cut(s) 86, 193, 1104, 1414
NsiI ATGCAT 1 cut(s) 183
NspI RCATGY 3 cut(s) 280, 889, 932
PceI AGGCCT 1 cut(s) 1409
PciI ACATGT 1 cut(s) 276
PfeI GAWTC 4 cut(s) 496, 555, 562, 710
PfoI TCCNGGA 3 cut(s) 189, 705, 1260
PkrI GCNGC 4 cut(s) 367, 891, 1195, 1465
PleI GAGTC 2 cut(s) 20, 229
PmaCI CACGTG 1 cut(s) 1319
PmlI CACGTG 1 cut(s) 1319
PpsI GAGTC 2 cut(s) 20, 229
Ppu21I YACGTR 1 cut(s) 1319
PscI ACATGT 1 cut(s) 276
PshAI GACNNNNGTC 1 cut(s) 1037
Psp124BI GAGCTC 1 cut(s) 1330
Psp6I CCWGG 5 cut(s) 189, 328, 644, 705, 1114
PspCI CACGTG 1 cut(s) 1319
PspFI CCCAGC 1 cut(s) 1288
PspGI CCWGG 5 cut(s) 189, 328, 644, 705, 1114
PspPI GGNCC 2 cut(s) 642, 1118
PvuII CAGCTG 1 cut(s) 1463
RsaI GTAC 1 cut(s) 1089
RsaNI GTAC 1 cut(s) 1088
RseI CAYNNNNRTG 1 cut(s) 864
SacI GAGCTC 1 cut(s) 1330
SaqAI TTAA 7 cut(s) 282, 474, 779, 797, 842, 873, 968
SatI GCNGC 4 cut(s) 366, 890, 1194, 1464
Sau3AI GATC 4 cut(s) 86, 193, 1104, 1414
Sau96I GGNCC 2 cut(s) 642, 1118
SchI GAGTC 2 cut(s) 21, 229
ScrFI CCNGG 6 cut(s) 191, 330, 646, 707, 1116, 1262
SduI GDGCHC 1 cut(s) 1330
SfaNI GCATC 9 cut(s) 218, 577, 610, 859, 964, 1008, 1009, 1120, 1344
SfcI CTRYAG 1 cut(s) 948
SinI GGWCC 2 cut(s) 642, 1118
SmiMI CAYNNNNRTG 1 cut(s) 864
SmlI CTYRAG 4 cut(s) 44, 321, 979, 1432
SmoI CTYRAG 4 cut(s) 44, 321, 979, 1432
SseBI AGGCCT 1 cut(s) 1409
SsiI CCGC 1 cut(s) 721
SspI AATATT 2 cut(s) 439, 1156
SstI GAGCTC 1 cut(s) 1330
StuI AGGCCT 1 cut(s) 1409
StyD4I CCNGG 6 cut(s) 189, 328, 644, 705, 1114, 1260
StyI CCWWGG 2 cut(s) 569, 933
TaaI ACNGT 2 cut(s) 1171, 1441
TaiI ACGT 3 cut(s) 361, 703, 1321
TaqI TCGA 4 cut(s) 85, 426, 432, 855
TatI WGTACW 1 cut(s) 1087
TfiI GAWTC 4 cut(s) 496, 555, 562, 710
Tru1I TTAA 7 cut(s) 282, 474, 779, 797, 842, 873, 968
Tru9I TTAA 7 cut(s) 282, 474, 779, 797, 842, 873, 968
TscAI CASTG 6 cut(s) 375, 453, 506, 1010, 1176, 1213
TseI GCWGC 4 cut(s) 365, 889, 1193, 1463
TspDTI ATGAA 8 cut(s) 225, 354, 647, 1289, 1326, 1542, 1631, 1662
TspRI CASTG 6 cut(s) 375, 453, 506, 1010, 1176, 1213
VpaK11BI GGWCC 2 cut(s) 642, 1118
XapI RAATTY 5 cut(s) 79, 409, 1234, 1494, 1651
XceI RCATGY 3 cut(s) 280, 889, 932
Zsp2I ATGCAT 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.