MD14G1070800.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003524
Physical Location & Seq
Forward (+)
7786472 .. 7800010
13539 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1070800.v1.1.491

Sequence Viewer

Length: 3090 bp
ATGAGCGCTGTTTCGGGGGTGATTTCGCGCCAAGTTTTGCCTGCCTGTGGCAGCCTTTGCTTCTTCTGCCCGGCATTGCGAGCCAGGTCTAGACAGCCGGTTAAGAGGTACAAGAAGCTCATTGCTGATATTTTCCCTCGGAACCAGGAAGAGGGACCTAATGATCGGAAGATTGGGAAGTTGTGCGAATATGTTGCCAAAAATCCTTTGCGAATCCCAAAGATTACAACTTATCTTGAGCAAAGGTGCTACAAAGAATTGAGGAATGAGAACTTTCGATCCGCAAAAATTGTAATGTGCATTTACAACAAATTGCTGATTTCTTGTAAGGAGCAAATGCCTCTTTTTGCGAGTAGCTTGCTCAGCATTATGCATACACTTCTGGATCAAACACGGCAAGATGAAATGCAAATTATAGGATGTCAGACTCTTTTCAACTTTGTAAATAATCAGACGGATGGAACTTACACGTTCAACTTAGAAGGCTTTATTCCAAAACTCTGTCAGATAGCTCAAGAGCCGGGGGAAGATGAAAGGGCAAGTAATCTACGTTCAGCTGCACTCCAAGCCCTTTCTTCAATGGTTTGGTTTATGGGTGAGAAGTCTCATATATCAGTCAAGTTCGATAGCATTGTTGCAGTTGTCTTGGAAAATTATGGAGGTTCAAATAAAACTACAGAGAACCTTGAAGGCTCAAAGCGGTGGGTGCAGGAAGTGCAAAGTAACGAGGGTCATGGTTCTTCTTCCCCAGATGTCACCATCAGGCTTACATCTTGGAGCACCCTTGTGGATGACAAAGGCGAACTAAATGTGACAGTGGAAGATGCCAAGAACCCCTGCTTTTGGTCTAGGGTTTGCCTGCACAACATGGCCAAGCTAGCCAAGGAGGCTACAACAATCAGACGTGTCCTGGAATCTCTGTTCCGTTACTTCGACACAGGGAACTTATGGTCTCCCAAATATGGTCTTGCTATCCCAGTCCTAAAGGATATACAAGTTTTAATGGATGATTGTGGGCAAAGTACACATATTTTGCTTTCCATTCTAATCAAGCATTTGGATCACAAAAATGTCCTCAAACAACCCAACATGCAGCTCGAAATAGCGGAGGTTACCACCTCTCTTTCTCAACTTGCCAAGGTTGAGCCCTCTGTGGCAATAATTGGTGCAGTAAGTGATGCCATGAGGCATTTGAGGAAGAGCATTCACTGCTCGCTTGATGATGCTAATTTGGGAACAGATGTAACAAAATGGAACAGAAGCTTTAGAGAAGTGGTGGATAAGTGCCTTGTACAGTTATGTTATAAGGTTGGAGAACCAGGACCAATCCTTGATGCTATGGCTGTTATGTTGGAAAATATCTCGACTATTACAGTTATAGCCAGAAATACAATCTCTGCTGTTTATCGTACAGCTCAAATTGTAGCTTCCTTACCAAATTTGTCCTATCAAAACAAGGCTTTCCCTGAGGCATTGTTTCATCAATTACTGCCAGCTATGGTCCATCCAGACCATGAAACACGTGTTGGAGCACATCGCATCTTCTCTGTTGTTCTTGTGCCATCTTCAGTTTGTCCTACTCGATCCTCACCTAATACTGAGTCAAAAAAGGCATTGAATTTTCCCAGGACACTCTCAAGAACTGTATCTGTCTTTTCTTCTTCAGCTGCCCTCTTTGAGAAGCTAAGGAGAGAGAAAATTTCTTCACGAGAAAGTATTTGTGAAGAGGACAATGAAAATACAGATAATGAGGGGGAACAAAGAGATACCAATAATGGGATTCTGAGCAGATTGAGGTCTTCTTATAGTCGGACATATAGCATAAAAAGTTCTCCTGCACCTTCATCAACAAAAGAAAATTCTGTCAACAGTTCGACTAAGGAACCTGAAGCTAATTCCCTCAGATTGAGCAGCCACCAGATAACTCTCTTGCTTTTATCAATTTGGGCGCAATCCATCTCTCCCGGGAACATGCCGGAAAACTATGAAGCAATTGCTCATACGCTTAGCCTCGTAGTGCTGTTTTCTCGGGCCAAGAATTCCAGAATTGAGGTTCTTGTTCGAAGTTTTCAACTGGCATTTTCCTTGCGGAACATATCTCTTAATGAAGGAGGGCTGCTGCCACCATCCCGTCGCAGGTCCCTTTTTACCCTTGCAACTTCAATGATTCTCTTTCTATCAAAAGCATACAATATTGTTTCTCTTGTTTGTAGAGCCAAGGCGGTACTTGTGGATAAAATAGTTGATCCGTTCCTGCATTTGGTTGAAGATCGCAAGTTACAGGCTGTCAAGACTGGACCGAACCATCCCAGACATCTTTATGGATCTGAAGAAGATGATAATATGGCATTAAAATCTCTATCAGAGATCAATATTACTGAAGAGCAAACCAAGGAATTTTTTGCTTCTGAAGTTGTGAAGAGCTTGGAAAGATTGTTAGATGCTGAAATGTCTACTATAAGGGAGGAGCTGCTCAGTGAATTCTTACCTGATGATGTGTGTCCACTGGGAGCCCAGTTGTGTATGGATGCCCCGCAAAAGTTGTATCAGGTTGATTCGAGGGACACTAAATCTATGAAAGAGGATGCTCCAATATTTTCATTGGACGATGATTATTTTCAAGGTTCATTTGATAGCCAAAAGAACAACTTGGATTTTTCTGCAGAGTCTCACAATCTTTTGAGTGTCAGTCAACTTATTGAATCAGTCCTGGAGACCGCACATCAAGTTGGAAGAGTATCGGTCTCAAATGCACCTGATGTGCCATACAAGGAGATGGCCGGCCAGTGTGAGGCACTCCTGTTAGGAAAGCAGCAGAAGATGTCAAATTTGATGAGTTCCCAACCGAAGCAGGAATATTTGATGAATCAGTCTTTACAAAATCACAGTGATGACGCCAAATGGATGACATCCGACTCACATAGGAGCGGAAATCCATTTGTTGACGACACTGCCAATTCATACAAACCATCCCCTAGCACAGGCCATGCCCCGATGATGTGTGTAACCGAATACCAGCATCATCCGCACTCTTTCAGACTACCAGCGTCGAGTCCGTATGATAACTTCCTAAAAGCTGCAGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1030

Amino Acids

115.15

Weight (kDa)

6.72

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 69 - 159 1.7e-19 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1305
Acc36I ACCTGC 2 cut(s) 2127, 3071
AccBSI CCGCTC 1 cut(s) 2928
AccI GTMKAC 1 cut(s) 2453
AccII CGCG 1 cut(s) 28
AciI CCGC 9 cut(s) 282, 700, 1106, 2089, 2222, 2534, 2718, 2928, 3026
AclWI GGATC 6 cut(s) 273, 393, 1068, 1578, 2240, 2332
AcoI YGGCCR 3 cut(s) 870, 2778, 2782
AcsI RAATTY 8 cut(s) 1438, 1618, 1698, 1858, 2038, 2396, 2480, 2827
AcuI CTGAAG 6 cut(s) 1551, 1647, 1908, 2349, 2400, 2430
AcvI CACGTG 1 cut(s) 1523
AcyI GRCGYC 1 cut(s) 2895
AfaI GTAC 5 cut(s) 110, 1024, 1293, 1411, 2226
AfeI AGCGCT 1 cut(s) 7
AfiI CCNNNNNNNGG 9 cut(s) 47, 151, 843, 962, 1776, 2136, 2260, 2791, 2981
AflIII ACRYGT 4 cut(s) 468, 904, 1520, 1522
AjiI CACGTC 1 cut(s) 905
AjnI CCWGG 6 cut(s) 83, 144, 909, 1318, 1625, 2709
AjuI GAANNNNNNNTTGG 2 cut(s) 1509, 1541
AleI CACNNNNGTG 1 cut(s) 785
AloI GAACNNNNNNTCC 4 cut(s) 263, 295, 905, 937
Alw21I GWGCWC 2 cut(s) 782, 1534
Alw26I GTCTC 5 cut(s) 609, 957, 2673, 2708, 2749
AlwI GGATC 6 cut(s) 273, 393, 1068, 1578, 2240, 2332
Ama87I CYCGRG 2 cut(s) 1964, 2028
Aor51HI AGCGCT 1 cut(s) 7
AoxI GGCC 5 cut(s) 870, 2031, 2778, 2782, 2983
ApoI RAATTY 8 cut(s) 1438, 1618, 1698, 1858, 2038, 2396, 2480, 2827
AspLEI GCGC 3 cut(s) 8, 30, 1951
AspS9I GGNCC 6 cut(s) 155, 1322, 1501, 2031, 2139, 2297
AsuC2I CCSGG 4 cut(s) 71, 522, 1965, 1966
AsuHPI GGTGA 4 cut(s) 31, 608, 748, 1581
AsuII TTCGAA 1 cut(s) 2062
AsuNHI GCTAGC 1 cut(s) 877
AvaI CYCGRG 2 cut(s) 1964, 2028
AvaII GGWCC 5 cut(s) 155, 1322, 1501, 2139, 2297
AxyI CCTNAGG 1 cut(s) 1467
BalI TGGCCA 1 cut(s) 872
BanII GRGCYC 2 cut(s) 1149, 2515
BarI GAAGNNNNNNTAC 2 cut(s) 3050, 3082
BauI CACGAG 1 cut(s) 1707
BbrPI CACGTG 1 cut(s) 1523
BbsI GAAGAC 1 cut(s) 1791
Bbv12I GWGCWC 2 cut(s) 782, 1534
BccI CCATC 9 cut(s) 452, 767, 1512, 1570, 1964, 2134, 2313, 2770, 2977
BceAI ACGGC 1 cut(s) 410
BcgI CGANNNNNNTGC 4 cut(s) 176, 210, 340, 374
BciT130I CCWGG 6 cut(s) 85, 146, 911, 1320, 1627, 2711
BcnI CCSGG 4 cut(s) 71, 522, 1965, 1966
BcoDI GTCTC 5 cut(s) 609, 957, 2673, 2708, 2749
BfaI CTAG 4 cut(s) 90, 849, 878, 2976
BfmI CTRYAG 3 cut(s) 675, 2661, 3078
BfoI RGCGCY 1 cut(s) 9
BfuAI ACCTGC 2 cut(s) 2127, 3071
BglI GCCNNNNNGGC 1 cut(s) 887
BlpI GCTNAGC 2 cut(s) 362, 2006
Bme18I GGWCC 5 cut(s) 155, 1322, 1501, 2139, 2297
BmeT110I CYCGRG 2 cut(s) 1964, 2028
BmgBI CACGTC 1 cut(s) 905
BmgT120I GGNCC 6 cut(s) 155, 1322, 1501, 2031, 2139, 2297
BmiI GGNNCC 5 cut(s) 143, 156, 1884, 2141, 2512
BmrI ACTGGG 3 cut(s) 971, 2509, 2516
BmsI GCATC 9 cut(s) 814, 1168, 1213, 1324, 1548, 2431, 2518, 2575, 3028
BmtI GCTAGC 1 cut(s) 881
BmuI ACTGGG 3 cut(s) 971, 2509, 2516
BpiI GAAGAC 1 cut(s) 1791
BpmI CTGGAG 1 cut(s) 2732
Bpu10I CCTNAGC 1 cut(s) 1685
Bpu1102I GCTNAGC 2 cut(s) 362, 2006
Bpu14I TTCGAA 1 cut(s) 2062
BpuEI CTTGAG 3 cut(s) 257, 498, 1621
BpuMI CCSGG 4 cut(s) 71, 522, 1965, 1966
BsaAI YACGTR 1 cut(s) 1523
BsaHI GRCGYC 1 cut(s) 2895
BsaI GGTCTC 3 cut(s) 957, 2708, 2749
BsaJI CCNNGG 8 cut(s) 137, 521, 882, 1137, 1625, 1964, 2217, 2391
Bsc4I CCNNNNNNNGG 9 cut(s) 47, 151, 843, 962, 1776, 2136, 2260, 2791, 2981
Bse118I RCCGGY 2 cut(s) 97, 2780
Bse1I ACTGG 6 cut(s) 977, 2079, 2299, 2511, 2515, 2785
Bse21I CCTNAGG 1 cut(s) 1467
Bse3DI GCAATG 2 cut(s) 74, 120
BseBI CCWGG 6 cut(s) 85, 146, 911, 1320, 1627, 2711
BseDI CCNNGG 8 cut(s) 137, 521, 882, 1137, 1625, 1964, 2217, 2391
BseLI CCNNNNNNNGG 9 cut(s) 47, 151, 843, 962, 1776, 2136, 2260, 2791, 2981
BseMI GCAATG 2 cut(s) 74, 120
BseMII CTCAG 6 cut(s) 376, 1458, 1590, 1775, 1915, 2488
BseNI ACTGG 6 cut(s) 977, 2079, 2299, 2511, 2515, 2785
BseRI GAGGAG 1 cut(s) 2480
BsgI GTGCAG 5 cut(s) 543, 728, 845, 1188, 1821
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 5 cut(s) 872, 2033, 2780, 2784, 2985
BsiHKAI GWGCWC 2 cut(s) 782, 1534
BsiHKCI CYCGRG 2 cut(s) 1964, 2028
BsiSI CCGG 6 cut(s) 71, 98, 521, 1965, 1976, 2781
BslFI GGGAC 3 cut(s) 168, 2125, 2576
BslI CCNNNNNNNGG 9 cut(s) 47, 151, 843, 962, 1776, 2136, 2260, 2791, 2981
BsmAI GTCTC 5 cut(s) 609, 957, 2673, 2708, 2749
BsmFI GGGAC 3 cut(s) 168, 2125, 2576
BsmI GAATGC 1 cut(s) 1203
BsnI GGCC 5 cut(s) 872, 2033, 2780, 2784, 2985
Bso31I GGTCTC 3 cut(s) 957, 2708, 2749
BsoBI CYCGRG 2 cut(s) 1964, 2028
Bsp119I TTCGAA 1 cut(s) 2062
Bsp1286I GDGCHC 4 cut(s) 782, 1149, 1534, 2515
Bsp1407I TGTACA 1 cut(s) 1291
Bsp143I GATC 9 cut(s) 163, 278, 385, 1060, 1583, 2245, 2269, 2324, 2367
Bsp1720I GCTNAGC 2 cut(s) 362, 2006
BspACI CCGC 9 cut(s) 282, 700, 1106, 2089, 2222, 2534, 2718, 2928, 3026
BspANI GGCC 5 cut(s) 872, 2033, 2780, 2784, 2985
BspCNI CTCAG 6 cut(s) 375, 1459, 1591, 1776, 1914, 2487
BspFNI CGCG 1 cut(s) 28
BspLI GGNNCC 5 cut(s) 143, 156, 1884, 2141, 2512
BspMAI CTGCAG 2 cut(s) 2665, 3082
BspMI ACCTGC 2 cut(s) 2127, 3071
BspOI GCTAGC 1 cut(s) 881
BspPI GGATC 6 cut(s) 273, 393, 1068, 1578, 2240, 2332
BspQI GCTCTTC 3 cut(s) 1193, 2376, 2414
BspT104I TTCGAA 1 cut(s) 2062
BspTNI GGTCTC 3 cut(s) 957, 2708, 2749
BsrBI CCGCTC 1 cut(s) 2928
BsrDI GCAATG 2 cut(s) 74, 120
BsrFI RCCGGY 2 cut(s) 97, 2780
BsrGI TGTACA 1 cut(s) 1291
BsrI ACTGG 6 cut(s) 977, 2079, 2299, 2511, 2515, 2785
BssAI RCCGGY 2 cut(s) 97, 2780
BssECI CCNNGG 8 cut(s) 137, 521, 882, 1137, 1625, 1964, 2217, 2391
BssMI GATC 9 cut(s) 163, 278, 385, 1060, 1583, 2245, 2269, 2324, 2367
BssNI GRCGYC 1 cut(s) 2895
BssSI CACGAG 1 cut(s) 1707
BssT1I CCWWGG 4 cut(s) 882, 1137, 2217, 2391
Bst2BI CACGAG 1 cut(s) 1707
Bst2UI CCWGG 6 cut(s) 85, 146, 911, 1320, 1627, 2711
Bst4CI ACNGT 6 cut(s) 817, 1296, 1375, 1645, 1871, 2888
Bst6I CTCTTC 6 cut(s) 144, 1193, 1719, 2376, 2414, 2728
BstACI GRCGYC 1 cut(s) 2895
BstAPI GCANNNNNTGC 3 cut(s) 57, 715, 1209
BstAUI TGTACA 1 cut(s) 1291
BstBAI YACGTR 1 cut(s) 1523
BstBI TTCGAA 1 cut(s) 2062
BstC8I GCNNGC 8 cut(s) 42, 81, 359, 860, 879, 1214, 1494, 2782
BstEII GGTNACC 1 cut(s) 1111
BstENI CCTNNNNNAGG 1 cut(s) 2979
BstFNI CGCG 1 cut(s) 28
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 3 cut(s) 8, 30, 1951
BstKTI GATC 9 cut(s) 166, 281, 388, 1063, 1586, 2248, 2272, 2327, 2370
BstMAI GTCTC 5 cut(s) 609, 957, 2673, 2708, 2749
BstMBI GATC 9 cut(s) 163, 278, 385, 1060, 1583, 2245, 2269, 2324, 2367
BstNI CCWGG 6 cut(s) 85, 146, 911, 1320, 1627, 2711
BstNSI RCATGY 2 cut(s) 1093, 1975
BstPI GGTNACC 1 cut(s) 1111
BstSFI CTRYAG 3 cut(s) 675, 2661, 3078
BstUI CGCG 1 cut(s) 28
BstV2I GAAGAC 1 cut(s) 1791
BstX2I RGATCY 1 cut(s) 2324
BstYI RGATCY 1 cut(s) 2324
Bsu36I CCTNAGG 1 cut(s) 1467
BsuRI GGCC 5 cut(s) 872, 2033, 2780, 2784, 2985
BtgZI GCGATG 1 cut(s) 1520
BtrI CACGTC 1 cut(s) 905
BtsI GCAGTG 2 cut(s) 1207, 2949
BtsIMutI CAGTG 7 cut(s) 822, 1207, 2482, 2504, 2792, 2893, 2949
BveI ACCTGC 2 cut(s) 2127, 3071
Cac8I GCNNGC 8 cut(s) 42, 81, 359, 860, 879, 1214, 1494, 2782
CfoI GCGC 3 cut(s) 8, 30, 1951
Cfr10I RCCGGY 2 cut(s) 97, 2780
Cfr13I GGNCC 6 cut(s) 155, 1322, 1501, 2031, 2139, 2297
Cfr9I CCCGGG 1 cut(s) 1964
CseI GACGC 2 cut(s) 2903, 3036
Csp6I GTAC 5 cut(s) 109, 1023, 1292, 1410, 2225
CviAII CATG 7 cut(s) 734, 868, 1090, 1183, 1514, 1972, 2987
CviQI GTAC 5 cut(s) 109, 1023, 1292, 1410, 2225
DpnI GATC 9 cut(s) 165, 280, 387, 1062, 1585, 2247, 2271, 2326, 2369
DpnII GATC 9 cut(s) 163, 278, 385, 1060, 1583, 2245, 2269, 2324, 2367
EaeI YGGCCR 3 cut(s) 870, 2778, 2782
Eam1104I CTCTTC 6 cut(s) 144, 1193, 1719, 2376, 2414, 2728
EarI CTCTTC 6 cut(s) 144, 1193, 1719, 2376, 2414, 2728
Eco130I CCWWGG 4 cut(s) 882, 1137, 2217, 2391
Eco24I GRGCYC 2 cut(s) 1149, 2515
Eco31I GGTCTC 3 cut(s) 957, 2708, 2749
Eco47I GGWCC 5 cut(s) 155, 1322, 1501, 2139, 2297
Eco47III AGCGCT 1 cut(s) 7
Eco57I CTGAAG 6 cut(s) 1551, 1647, 1908, 2349, 2400, 2430
Eco72I CACGTG 1 cut(s) 1523
Eco81I CCTNAGG 1 cut(s) 1467
Eco88I CYCGRG 2 cut(s) 1964, 2028
Eco91I GGTNACC 1 cut(s) 1111
EcoNI CCTNNNNNAGG 1 cut(s) 2979
EcoO109I RGGNCCY 2 cut(s) 155, 2139
EcoO65I GGTNACC 1 cut(s) 1111
EcoRI GAATTC 2 cut(s) 2038, 2480
EcoRII CCWGG 6 cut(s) 83, 144, 909, 1318, 1625, 2709
EcoT14I CCWWGG 4 cut(s) 882, 1137, 2217, 2391
EcoT22I ATGCAT 1 cut(s) 375
EcoT38I GRGCYC 2 cut(s) 1149, 2515
ErhI CCWWGG 4 cut(s) 882, 1137, 2217, 2391
FaeI CATG 7 cut(s) 737, 871, 1093, 1186, 1517, 1975, 2990
FalI AAGNNNNNCTT 2 cut(s) 2633, 2665
FaqI GGGAC 3 cut(s) 168, 2125, 2576
FatI CATG 7 cut(s) 733, 867, 1089, 1182, 1513, 1971, 2986
FauI CCCGC 1 cut(s) 2541
FblI GTMKAC 1 cut(s) 2453
FriOI GRGCYC 2 cut(s) 1149, 2515
FseI GGCCGGCC 1 cut(s) 2784
FspBI CTAG 4 cut(s) 90, 849, 878, 2976
GlaI GCGC 3 cut(s) 7, 29, 1950
GsuI CTGGAG 1 cut(s) 2732
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 5 cut(s) 872, 2033, 2780, 2784, 2985
HapII CCGG 6 cut(s) 71, 98, 521, 1965, 1976, 2781
HgaI GACGC 2 cut(s) 2903, 3036
HhaI GCGC 3 cut(s) 8, 30, 1951
Hin1I GRCGYC 1 cut(s) 2895
Hin1II CATG 7 cut(s) 737, 871, 1093, 1186, 1517, 1975, 2990
Hin6I GCGC 3 cut(s) 6, 28, 1949
HinP1I GCGC 3 cut(s) 6, 28, 1949
HincII GTYRAC 3 cut(s) 1867, 2693, 2944
HindII GTYRAC 3 cut(s) 1867, 2693, 2944
HindIII AAGCTT 1 cut(s) 1261
HpaII CCGG 6 cut(s) 71, 98, 521, 1965, 1976, 2781
HphI GGTGA 4 cut(s) 31, 608, 748, 1581
Hpy166II GTNNAC 6 cut(s) 1025, 1867, 2454, 2504, 2693, 2944
Hpy8I GTNNAC 6 cut(s) 1025, 1867, 2454, 2504, 2693, 2944
Hpy99I CGWCG 2 cut(s) 2136, 3052
HpyAV CCTTC 4 cut(s) 476, 683, 1851, 2102
HpyCH4III ACNGT 6 cut(s) 817, 1296, 1375, 1645, 1871, 2888
HpyCH4IV ACGT 4 cut(s) 470, 550, 904, 1522
HpySE526I ACGT 4 cut(s) 470, 550, 904, 1522
Hsp92I GRCGYC 1 cut(s) 2895
Hsp92II CATG 7 cut(s) 737, 871, 1093, 1186, 1517, 1975, 2990
HspAI GCGC 3 cut(s) 6, 28, 1949
KroI GCCGGC 1 cut(s) 2780
KroNI GCCGGC 1 cut(s) 2782
Kzo9I GATC 9 cut(s) 163, 278, 385, 1060, 1583, 2245, 2269, 2324, 2367
LguI GCTCTTC 3 cut(s) 1193, 2376, 2414
LmnI GCTCC 7 cut(s) 331, 777, 1529, 2467, 2510, 2593, 2925
LweI GCATC 9 cut(s) 814, 1168, 1213, 1324, 1548, 2431, 2518, 2575, 3028
MaeI CTAG 4 cut(s) 90, 849, 878, 2976
MaeII ACGT 4 cut(s) 470, 550, 904, 1522
MaeIII GTNAC 8 cut(s) 722, 754, 811, 926, 1111, 1243, 2277, 3004
MalI GATC 9 cut(s) 165, 280, 387, 1062, 1585, 2247, 2271, 2326, 2369
MbiI CCGCTC 1 cut(s) 2928
MboI GATC 9 cut(s) 163, 278, 385, 1060, 1583, 2245, 2269, 2324, 2367
MfeI CAATTG 1 cut(s) 1992
MflI RGATCY 1 cut(s) 2324
MhlI GDGCHC 4 cut(s) 782, 1149, 1534, 2515
MlsI TGGCCA 1 cut(s) 872
MluNI TGGCCA 1 cut(s) 872
MlyI GAGTC 5 cut(s) 421, 1610, 2675, 2909, 3061
MmeI TCCRAC 6 cut(s) 1291, 1332, 1507, 1790, 2710, 2937
Mox20I TGGCCA 1 cut(s) 872
Mph1103I ATGCAT 1 cut(s) 375
MroNI GCCGGC 1 cut(s) 2780
MscI TGGCCA 1 cut(s) 872
MseI TTAA 4 cut(s) 102, 1001, 2103, 2351
MslI CAYNNNNRTG 4 cut(s) 785, 1068, 2319, 2889
Msp20I TGGCCA 1 cut(s) 872
MspA1I CMGCKG 2 cut(s) 557, 1667
MspI CCGG 6 cut(s) 71, 98, 521, 1965, 1976, 2781
MunI CAATTG 1 cut(s) 1992
Mva1269I GAATGC 1 cut(s) 1203
MvaI CCWGG 6 cut(s) 85, 146, 911, 1320, 1627, 2711
MvnI CGCG 1 cut(s) 28
NaeI GCCGGC 1 cut(s) 2782
NciI CCSGG 4 cut(s) 71, 522, 1965, 1966
NdeII GATC 9 cut(s) 163, 278, 385, 1060, 1583, 2245, 2269, 2324, 2367
NgoMIV GCCGGC 1 cut(s) 2780
NheI GCTAGC 1 cut(s) 877
NlaIII CATG 7 cut(s) 737, 871, 1093, 1186, 1517, 1975, 2990
NlaIV GGNNCC 5 cut(s) 143, 156, 1884, 2141, 2512
NmuCI GTSAC 2 cut(s) 754, 811
NsiI ATGCAT 1 cut(s) 375
NspI RCATGY 2 cut(s) 1093, 1975
NspV TTCGAA 1 cut(s) 2062
OliI CACNNNNGTG 1 cut(s) 785
PciSI GCTCTTC 3 cut(s) 1193, 2376, 2414
PctI GAATGC 1 cut(s) 1203
PdiI GCCGGC 1 cut(s) 2782
PfeI GAWTC 7 cut(s) 213, 914, 1780, 2167, 2555, 2702, 2866
PfoI TCCNGGA 2 cut(s) 909, 2709
PleI GAGTC 5 cut(s) 421, 1609, 2674, 2909, 3060
PmaCI CACGTG 1 cut(s) 1523
PmlI CACGTG 1 cut(s) 1523
PpsI GAGTC 5 cut(s) 421, 1609, 2674, 2909, 3060
Ppu21I YACGTR 1 cut(s) 1523
PpuMI RGGWCCY 2 cut(s) 155, 2139
PsiI TTATAA 1 cut(s) 1305
Psp5II RGGWCCY 2 cut(s) 155, 2139
Psp6I CCWGG 6 cut(s) 83, 144, 909, 1318, 1625, 2709
PspCI CACGTG 1 cut(s) 1523
PspEI GGTNACC 1 cut(s) 1111
PspGI CCWGG 6 cut(s) 83, 144, 909, 1318, 1625, 2709
PspN4I GGNNCC 5 cut(s) 143, 156, 1884, 2141, 2512
PspPI GGNCC 6 cut(s) 155, 1322, 1501, 2031, 2139, 2297
PspPPI RGGWCCY 2 cut(s) 155, 2139
PsrI GAACNNNNNNTAC 2 cut(s) 535, 567
PstI CTGCAG 2 cut(s) 2665, 3082
PsuI RGATCY 1 cut(s) 2324
PvuII CAGCTG 2 cut(s) 557, 1667
RigI GGCCGGCC 1 cut(s) 2784
RsaI GTAC 5 cut(s) 110, 1024, 1293, 1411, 2226
RsaNI GTAC 5 cut(s) 109, 1023, 1292, 1410, 2225
RseI CAYNNNNRTG 4 cut(s) 785, 1068, 2319, 2889
SapI GCTCTTC 3 cut(s) 1193, 2376, 2414
SaqAI TTAA 4 cut(s) 102, 1001, 2103, 2351
Sau3AI GATC 9 cut(s) 163, 278, 385, 1060, 1583, 2245, 2269, 2324, 2367
Sau96I GGNCC 6 cut(s) 155, 1322, 1501, 2031, 2139, 2297
SchI GAGTC 5 cut(s) 421, 1610, 2675, 2909, 3061
SduI GDGCHC 4 cut(s) 782, 1149, 1534, 2515
SfaNI GCATC 9 cut(s) 814, 1168, 1213, 1324, 1548, 2431, 2518, 2575, 3028
SfcI CTRYAG 3 cut(s) 675, 2661, 3078
SfuI TTCGAA 1 cut(s) 2062
SinI GGWCC 5 cut(s) 155, 1322, 1501, 2139, 2297
SmaI CCCGGG 1 cut(s) 1966
SmiMI CAYNNNNRTG 4 cut(s) 785, 1068, 2319, 2889
SmlI CTYRAG 3 cut(s) 236, 513, 1636
SmoI CTYRAG 3 cut(s) 236, 513, 1636
SsiI CCGC 9 cut(s) 282, 700, 1106, 2089, 2222, 2534, 2718, 2928, 3026
SspI AATATT 4 cut(s) 2194, 2374, 2595, 2858
SspMI CTAG 4 cut(s) 90, 849, 878, 2976
StyI CCWWGG 4 cut(s) 882, 1137, 2217, 2391
TaaI ACNGT 6 cut(s) 817, 1296, 1375, 1645, 1871, 2888
TaiI ACGT 4 cut(s) 473, 553, 907, 1525
TaqII GACCGA 2 cut(s) 2314, 2731
TatI WGTACW 2 cut(s) 1022, 1291
TfiI GAWTC 7 cut(s) 213, 914, 1780, 2167, 2555, 2702, 2866
Tru1I TTAA 4 cut(s) 102, 1001, 2103, 2351
Tru9I TTAA 4 cut(s) 102, 1001, 2103, 2351
TscAI CASTG 7 cut(s) 822, 1214, 2482, 2511, 2792, 2893, 2956
TseFI GTSAC 2 cut(s) 754, 811
Tsp45I GTSAC 2 cut(s) 754, 811
TspGWI ACGGA 4 cut(s) 470, 914, 2238, 3045
TspMI CCCGGG 1 cut(s) 1964
TspRI CASTG 7 cut(s) 822, 1214, 2482, 2511, 2792, 2893, 2956
VpaK11BI GGWCC 5 cut(s) 155, 1322, 1501, 2139, 2297
XagI CCTNNNNNAGG 1 cut(s) 2979
XapI RAATTY 8 cut(s) 1438, 1618, 1698, 1858, 2038, 2396, 2480, 2827
XbaI TCTAGA 1 cut(s) 89
XceI RCATGY 2 cut(s) 1093, 1975
XmaI CCCGGG 1 cut(s) 1964
XmiI GTMKAC 1 cut(s) 2453
XspI CTAG 4 cut(s) 90, 849, 878, 2976
Zsp2I ATGCAT 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.