Rorug03G0194100

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003524
Physical Location & Seq
Forward (+)
17057411 .. 17060840
3430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0194100.1

Sequence Viewer

Length: 3330 bp
ATGTCGGAGGATACTCATGAATCGGTTGATGTTGATGCCTCTGCATCTCTTCCTTCATCATCAGCTCCTCTGTGGAAGCATGATGTGTTTTTGAGTTTCAGGGGTGCAGACACTCGAAAAGGTATTGCATCGGAAATATACTATCGACTGCAAAACAGGAGAGGAATCAAAACATTCATGGATGACCAAGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCTCAAAACTACGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGACACATTTGTGAATGCATGGAAGAAGTGGAAGAAGACAAAAACAGAATTCTGCCACTTTTTTATTATGTGGATCCTACTAATGTACGACATCAGAAGAGGAGTTTTGGAGATGCTTTCGCTGAGCATGATAAATCTGGGCGACACGGATTAGAGAAGGTGCAGCAGTGGAGAGCTGCTTTAAAAAAAGTGGCAAATTTCTCTGGGTGGAACACACAGGATTATAAAACTGAAAGAGAACTTTTGGATGCCATTGAGGAATTTGTGTGCAATAAATTACGAGCTACTGAACTTGAGTTTACAATGTCCATCGGAGGTTTTGAAGCATTTGAAGCAACAAAACAAGCCATTGATCAGGTCATGAATGCGCTGAAAGATAGTAAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTCGGGAAGACGACAATGGTGAATTATGTCGGTGTACAAGCCCAAGAAAATAGGCTTTTTGATAAAGTGATTTTGGCTGTCGTATCCCAAATCCCCGACTTGATGAAAATTCAACAGACATTTGCAGAAATGCTGCGCTTGGAACTCAAGGAGAATACAGAAATTGTAAGAGCCCGTGAATTGAAGAAGAAAATAATGAGAGAAACCAAGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGAGTTTTCAGACATTGGAATTCCGAGCCACGGTGAACTTCAAAGATGCAATTCCAAAGTTCTACTGACCACCAGAAAATTGAGTGTTTGCTACTCCATGGATTGCCATGCAAACATTCATCTCAATATCTTATCAGAAGAAGATTCTTGGAGCTTATTCGTGAAGGAAGCAAGGAAGTCTTTTGACAAATCATCCAATTTCAATGCTGTAGCACATCAGGTGGCTAGAGAATGCGCGGGTCTGCCAGTTGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGATGAAGGTTTAGATGGATGGAAAGAAGCTGCTCGACGACTACAAGAGTCTCAACCTGCAAACCCTGAAGATGAGGAAGATGTGTACAGATGCATCAAGTTAAGCTATGATTACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGTTCAAGTATGGAATTGGGAAAGGAATGTTTCAAGATTCAAACTTACTAGAAGCCCGAGCCACAACTTATTTAGTGGTGAAGGCCCTTAAAGATTCTAGCCTGCTTTTGGAAGCTAGAAATGACAGATATGTAAAGATGCATGATGTCGTTCGGGATATGGCAATCTCAATTTCGTTATCTGAAGGCCAGCGGGTCTTGGTGAAAGTTGGCTGTGAATTGAAGAATTGGCCACAGATTGATGCACATAAAGGCTACACTGGAATCTCACTAATGGAGAACGAAATTTGGAAGCTACCCCAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTGCAAGACAATGATCGTCTATATGAGATCCCAGACTCTTTTTTCCAAAGTCAGAATGAATTAAGAGTCTTGGATCTTAGCGGCACTGGTATTTCATTACTACCCCAATCAATCAGTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCGAGAACATAATTGACATTTCTGTAATCGGAAAACTTAACAAGCTTGAGATTCTTAGTATGAGAGGAATTGCTCTGAGAGAATTGCCAAGAGAAATAGGACAGTTGACCAATCTAAGGATGCTGGATGTCAGTACTTTTCCAGACGAGGACGATGTACCCGACGAAGTTATCGCCACCGCAACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGCACTGTACCTAGAACGTGGATGTAGGGATTGGAGGAGTAAAATTGATGAAGAAGGAGAAGAAACCAATATTGTCTTTGATGAAGTAGTTGGTTTATCATATTTAAGCATTTTGCAAGTTTGCATATCAGATGCAAATTGCATCCCTAGAAGTGTTGAGGTCGAACCGAATTGGGTTTACTTCGATATTTGCATTTGCAGCAACAGTGGTGGACAGAAGGAAATGGTGAGCTCTCTATCTGGTGAAAGTTATAATCGTAGGGACTTGGGTCTTGACACAACCATAGGAACCCTTCCGGACTGGTTTGTCAACTGTGTGGTAAAGAATACTGAGACGCTAGATTATGCTTATTGCAGAGGGTTGAGTAACATTCTTGTGGAATATGACCGTGGGAGGTTAAATGGACAGAAAGTTCTCCATGTACGTGGTTGCTGTGAGAACTTGAAAGAGTTGATGAATGCAATAACATGTGTTCCTGATAAGCCTGTGTTTGAGAACTTGGAAGAGTTGCATCTGTATTGGCTGGATGAACTGAAGCAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCCGCGGTCTGAAATTACTGAATGTCAGATCGTGTCGTAACTTGGGGAATGTGCTGTTGCCATCAAAATTGTTGCAGAAACTACCAAATCTGGAAGAACTAATCTGTGAGGCAAGTGGAGTGGAATATGTGTTTGGATGTGAAGGATTTGAGCCAGTTCAAACAAATCTGAGAGAGATGGAGTTGCGTTATCTAGACGTAATAAGAAGCTTATGTAATGGTCATGCTCCACGTGGAATGTTCCAGGCTCTAAAGAAATTGGCCATTGAGAGGTGCAACATCCAGGGAAGTCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAACTGGAAACCCTTGTTGTATGCAGTTGCCCTGTGTTGGAAAGAGTAATCGAAGCAAGGAGGGAAACATTGAACAACAAGAAGACCGTTCTTCCAATATTGAAGAACTTAACTTTGACATTTCTTCCAATGTTGTACGAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAACCTTATATCTGTGGGAGTGCCCCCACTTGTCATTTCCACGCTCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTTGAAGTTGATTTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1109

Amino Acids

126.11

Weight (kDa)

5.1

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 26 - 199 1.1e-47 TIR domain
TIR_2 PF13676 29 - 129 4.6e-16 TIR domain
NB-ARC PF00931 217 - 382 1.8e-30 NB-ARC domain
LRR_8 PF13855 587 - 645 8.5e-09 Leucine rich repeat
LRR_14 PF23598 612 - 728 1e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 886 - 1027 8e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 968 - 1096 1.4e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 534, 2409
AasI GACNNNNNNGTC 1 cut(s) 2462
Acc36I ACCTGC 1 cut(s) 1315
AccB7I CCANNNNNTGG 1 cut(s) 1750
AccI GTMKAC 1 cut(s) 711
AccII CGCG 2 cut(s) 1208, 2733
AccIII TCCGGA 1 cut(s) 2452
AciI CCGC 6 cut(s) 1208, 1636, 1860, 2112, 2731, 2733
AclWI GGATC 6 cut(s) 377, 390, 931, 1801, 1860, 2731
AcoI YGGCCR 2 cut(s) 1673, 2988
AcsI RAATTY 6 cut(s) 357, 505, 569, 834, 990, 1728
AcuI CTGAAG 3 cut(s) 1338, 1647, 2711
AcvI CACGTG 1 cut(s) 2960
AcyI GRCGYC 1 cut(s) 726
AdeI CACNNNGTG 1 cut(s) 294
AfaI GTAC 8 cut(s) 396, 762, 1337, 2068, 2091, 2165, 2580, 3188
AfiI CCNNNNNNNGG 6 cut(s) 1001, 1552, 1750, 2049, 2997, 3088
AflIII ACRYGT 1 cut(s) 2624
AjnI CCWGG 3 cut(s) 2719, 2970, 3009
AjuI GAANNNNNNNTTGG 4 cut(s) 1376, 1408, 2844, 2876
AleI CACNNNNGTG 1 cut(s) 318
AloI GAACNNNNNNTCC 2 cut(s) 2553, 2585
Alw21I GWGCWC 1 cut(s) 2390
Alw26I GTCTC 3 cut(s) 1305, 2483, 3023
AlwI GGATC 6 cut(s) 377, 390, 931, 1801, 1860, 2731
Ama87I CYCGRG 1 cut(s) 1500
Aor13HI TCCGGA 1 cut(s) 2452
AoxI GGCC 6 cut(s) 693, 1242, 1527, 1630, 1673, 2988
ApaI GGGCCC 1 cut(s) 1246
ApeKI GCWGC 6 cut(s) 472, 485, 859, 1280, 1404, 2355
ApoI RAATTY 6 cut(s) 357, 505, 569, 834, 990, 1728
AspLEI GCGC 3 cut(s) 679, 864, 1208
AspS9I GGNCC 3 cut(s) 1242, 1243, 1528
AsuHPI GGTGA 7 cut(s) 757, 1016, 1534, 1657, 2395, 2411, 2720
AvaI CYCGRG 1 cut(s) 1500
BaeGI GKGCMC 2 cut(s) 1246, 3254
BalI TGGCCA 2 cut(s) 1675, 2990
BamHI GGATCC 1 cut(s) 382
BanII GRGCYC 3 cut(s) 901, 1246, 2390
BbrPI CACGTG 1 cut(s) 2960
BbsI GAAGAC 3 cut(s) 351, 740, 3140
Bbv12I GWGCWC 1 cut(s) 2390
BbvI GCAGC 6 cut(s) 472, 484, 846, 1267, 1391, 2367
BccI CCATC 6 cut(s) 626, 1259, 1263, 2131, 2797, 2899
BceAI ACGGC 1 cut(s) 246
BcgI CGANNNNNNTGC 2 cut(s) 3179, 3213
BciT130I CCWGG 3 cut(s) 2721, 2972, 3011
BciVI GTATCC 2 cut(s) 4, 820
BclI TGATCA 1 cut(s) 661
BcoDI GTCTC 3 cut(s) 1305, 2483, 3023
BfaI CTAG 8 cut(s) 1197, 1493, 1542, 1560, 2168, 2304, 2495, 2921
BfmI CTRYAG 1 cut(s) 1179
BfuAI ACCTGC 1 cut(s) 1315
BfuI GTATCC 2 cut(s) 4, 820
BisI GCNGC 7 cut(s) 473, 486, 860, 1281, 1405, 1861, 2356
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 7 cut(s) 474, 487, 861, 1282, 1406, 1862, 2357
BmcAI AGTACT 1 cut(s) 2068
Bme1390I CCNGG 3 cut(s) 2721, 2972, 3011
BmeT110I CYCGRG 1 cut(s) 1500
BmgT120I GGNCC 3 cut(s) 1242, 1243, 1528
BmiI GGNNCC 4 cut(s) 384, 1244, 2446, 3305
BmrFI CCNGG 3 cut(s) 2721, 2972, 3011
BoxI GACNNNNGTC 1 cut(s) 2424
BpiI GAAGAC 3 cut(s) 351, 740, 3140
BpmI CTGGAG 1 cut(s) 1751
Bpu1102I GCTNAGC 1 cut(s) 432
BpuEI CTTGAG 3 cut(s) 623, 857, 2000
BsaAI YACGTR 2 cut(s) 2582, 2960
BsaHI GRCGYC 1 cut(s) 726
BsaJI CCNNGG 5 cut(s) 1000, 1068, 2545, 2731, 3010
BsaWI WCCGGW 1 cut(s) 2452
Bsc4I CCNNNNNNNGG 6 cut(s) 1001, 1552, 1750, 2049, 2997, 3088
Bse1I ACTGG 6 cut(s) 1217, 1708, 1870, 2462, 2882, 3060
BseAI TCCGGA 1 cut(s) 2452
BseBI CCWGG 3 cut(s) 2721, 2972, 3011
BseDI CCNNGG 5 cut(s) 1000, 1068, 2545, 2731, 3010
BseLI CCNNNNNNNGG 6 cut(s) 1001, 1552, 1750, 2049, 2997, 3088
BseMII CTCAG 5 cut(s) 423, 2000, 2478, 2888, 3053
BseNI ACTGG 6 cut(s) 1217, 1708, 1870, 2462, 2882, 3060
BseRI GAGGAG 4 cut(s) 57, 424, 2206, 3265
BseSI GKGCMC 2 cut(s) 1246, 3254
BseXI GCAGC 6 cut(s) 472, 484, 846, 1267, 1391, 2367
BsgI GTGCAG 2 cut(s) 126, 491
Bsh1236I CGCG 2 cut(s) 1208, 2733
BshFI GGCC 6 cut(s) 695, 1244, 1529, 1632, 1675, 2990
BsiHKAI GWGCWC 1 cut(s) 2390
BsiHKCI CYCGRG 1 cut(s) 1500
BsiSI CCGG 1 cut(s) 2453
BslFI GGGAC 1 cut(s) 2432
BslI CCNNNNNNNGG 6 cut(s) 1001, 1552, 1750, 2049, 2997, 3088
BsmAI GTCTC 3 cut(s) 1305, 2483, 3023
BsmBI CGTCTC 1 cut(s) 2483
BsmFI GGGAC 1 cut(s) 2432
BsmI GAATGC 4 cut(s) 329, 679, 1208, 2620
BsnI GGCC 6 cut(s) 695, 1244, 1529, 1632, 1675, 2990
BsoBI CYCGRG 1 cut(s) 1500
Bsp120I GGGCCC 1 cut(s) 1242
Bsp1286I GDGCHC 4 cut(s) 901, 1246, 2390, 3254
Bsp13I TCCGGA 1 cut(s) 2452
Bsp1407I TGTACA 2 cut(s) 760, 1335
Bsp143I GATC 8 cut(s) 382, 661, 936, 1792, 1806, 1852, 2723, 2756
Bsp1720I GCTNAGC 1 cut(s) 432
Bsp19I CCATGG 1 cut(s) 1068
BspACI CCGC 6 cut(s) 1208, 1636, 1860, 2112, 2731, 2733
BspANI GGCC 6 cut(s) 695, 1244, 1529, 1632, 1675, 2990
BspCNI CTCAG 5 cut(s) 424, 2001, 2479, 2889, 3052
BspEI TCCGGA 1 cut(s) 2452
BspFNI CGCG 2 cut(s) 1208, 2733
BspHI TCATGA 2 cut(s) 16, 669
BspLI GGNNCC 4 cut(s) 384, 1244, 2446, 3305
BspMI ACCTGC 1 cut(s) 1315
BspPI GGATC 6 cut(s) 377, 390, 931, 1801, 1860, 2731
BsrGI TGTACA 2 cut(s) 760, 1335
BsrI ACTGG 6 cut(s) 1217, 1708, 1870, 2462, 2882, 3060
BssECI CCNNGG 5 cut(s) 1000, 1068, 2545, 2731, 3010
BssMI GATC 8 cut(s) 382, 661, 936, 1792, 1806, 1852, 2723, 2756
BssNI GRCGYC 1 cut(s) 726
BssT1I CCWWGG 1 cut(s) 1068
Bst2UI CCWGG 3 cut(s) 2721, 2972, 3011
Bst4CI ACNGT 7 cut(s) 1004, 2037, 2163, 2363, 2471, 2546, 3139
Bst6I CTCTTC 6 cut(s) 54, 289, 401, 959, 2655, 3026
BstACI GRCGYC 1 cut(s) 726
BstAUI TGTACA 2 cut(s) 760, 1335
BstBAI YACGTR 2 cut(s) 2582, 2960
BstC8I GCNNGC 2 cut(s) 1547, 1634
BstDEI CTNAG 9 cut(s) 308, 432, 1856, 1988, 2009, 2048, 2487, 2897, 3039
BstDSI CCRYGG 4 cut(s) 1000, 1068, 2545, 2731
BstFNI CGCG 2 cut(s) 1208, 2733
BstHHI GCGC 3 cut(s) 679, 864, 1208
BstKTI GATC 8 cut(s) 385, 664, 939, 1795, 1809, 1855, 2726, 2759
BstMAI GTCTC 3 cut(s) 1305, 2483, 3023
BstMBI GATC 8 cut(s) 382, 661, 936, 1792, 1806, 1852, 2723, 2756
BstMWI GCNNNNNNNGC 3 cut(s) 641, 1229, 2355
BstNI CCWGG 3 cut(s) 2721, 2972, 3011
BstNSI RCATGY 1 cut(s) 2628
BstPAI GACNNNNGTC 1 cut(s) 2424
BstSCI CCNGG 3 cut(s) 2719, 2970, 3009
BstSFI CTRYAG 1 cut(s) 1179
BstSLI GKGCMC 2 cut(s) 1246, 3254
BstUI CGCG 2 cut(s) 1208, 2733
BstV1I GCAGC 6 cut(s) 472, 484, 846, 1267, 1391, 2367
BstV2I GAAGAC 3 cut(s) 351, 740, 3140
BstX2I RGATCY 5 cut(s) 382, 936, 1806, 1852, 2723
BstXI CCANNNNNNTGG 1 cut(s) 2582
BstYI RGATCY 5 cut(s) 382, 936, 1806, 1852, 2723
BsuI GTATCC 2 cut(s) 4, 820
BsuRI GGCC 6 cut(s) 695, 1244, 1529, 1632, 1675, 2990
BtgI CCRYGG 4 cut(s) 1000, 1068, 2545, 2731
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 6 cut(s) 482, 1701, 1863, 2159, 2368, 3047
BveI ACCTGC 1 cut(s) 1315
Cac8I GCNNGC 2 cut(s) 1547, 1634
CciI TCATGA 2 cut(s) 16, 669
CfoI GCGC 3 cut(s) 679, 864, 1208
Cfr13I GGNCC 3 cut(s) 1242, 1243, 1528
Cfr42I CCGCGG 1 cut(s) 2734
CseI GACGC 2 cut(s) 715, 2500
Csp6I GTAC 8 cut(s) 395, 761, 1336, 2067, 2090, 2164, 2579, 3187
CspCI CAANNNNNGTGG 6 cut(s) 685, 720, 2347, 2382, 2829, 2864
CviQI GTAC 8 cut(s) 395, 761, 1336, 2067, 2090, 2164, 2579, 3187
DdeI CTNAG 9 cut(s) 308, 432, 1856, 1988, 2009, 2048, 2487, 2897, 3039
DpnI GATC 8 cut(s) 384, 663, 938, 1794, 1808, 1854, 2725, 2758
DpnII GATC 8 cut(s) 382, 661, 936, 1792, 1806, 1852, 2723, 2756
DraI TTTAAA 1 cut(s) 492
DraIII CACNNNGTG 1 cut(s) 294
DrdI GACNNNNNNGTC 1 cut(s) 2462
DseDI GACNNNNNNGTC 1 cut(s) 2462
EaeI YGGCCR 2 cut(s) 1673, 2988
Eam1104I CTCTTC 6 cut(s) 54, 289, 401, 959, 2655, 3026
EarI CTCTTC 6 cut(s) 54, 289, 401, 959, 2655, 3026
Ecl136II GAGCTC 1 cut(s) 2388
Eco130I CCWWGG 1 cut(s) 1068
Eco24I GRGCYC 3 cut(s) 901, 1246, 2390
Eco32I GATATC 2 cut(s) 1429, 2136
Eco53kI GAGCTC 1 cut(s) 2388
Eco57I CTGAAG 3 cut(s) 1338, 1647, 2711
Eco72I CACGTG 1 cut(s) 2960
Eco88I CYCGRG 1 cut(s) 1500
EcoICRI GAGCTC 1 cut(s) 2388
EcoO109I RGGNCCY 3 cut(s) 1242, 1243, 1528
EcoRI GAATTC 2 cut(s) 357, 990
EcoRII CCWGG 3 cut(s) 2719, 2970, 3009
EcoRV GATATC 2 cut(s) 1429, 2136
EcoT14I CCWWGG 1 cut(s) 1068
EcoT22I ATGCAT 3 cut(s) 329, 1346, 1587
EcoT38I GRGCYC 3 cut(s) 901, 1246, 2390
ErhI CCWWGG 1 cut(s) 1068
Esp3I CGTCTC 1 cut(s) 2483
FalI AAGNNNNNCTT 2 cut(s) 1135, 1167
FaqI GGGAC 1 cut(s) 2432
FauI CCCGC 2 cut(s) 1201, 1629
FbaI TGATCA 1 cut(s) 661
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 7 cut(s) 473, 486, 860, 1281, 1405, 1861, 2356
FriOI GRGCYC 3 cut(s) 901, 1246, 2390
Fsp4HI GCNGC 7 cut(s) 473, 486, 860, 1281, 1405, 1861, 2356
FspBI CTAG 8 cut(s) 1197, 1493, 1542, 1560, 2168, 2304, 2495, 2921
GlaI GCGC 3 cut(s) 678, 863, 1207
GluI GCNGC 7 cut(s) 473, 486, 860, 1281, 1405, 1861, 2356
GsuI CTGGAG 1 cut(s) 1751
HaeIII GGCC 6 cut(s) 695, 1244, 1529, 1632, 1675, 2990
HapII CCGG 1 cut(s) 2453
HgaI GACGC 2 cut(s) 715, 2500
HhaI GCGC 3 cut(s) 679, 864, 1208
Hin1I GRCGYC 1 cut(s) 726
Hin6I GCGC 3 cut(s) 677, 862, 1206
HinP1I GCGC 3 cut(s) 677, 862, 1206
HincII GTYRAC 2 cut(s) 2040, 2467
HindII GTYRAC 2 cut(s) 2040, 2467
HindIII AAGCTT 3 cut(s) 1914, 1976, 2935
HpaII CCGG 1 cut(s) 2453
HphI GGTGA 7 cut(s) 757, 1016, 1534, 1657, 2395, 2411, 2720
Hpy166II GTNNAC 9 cut(s) 609, 712, 761, 1007, 1336, 2040, 2335, 2369, 2467
Hpy8I GTNNAC 9 cut(s) 609, 712, 761, 1007, 1336, 2040, 2335, 2369, 2467
Hpy99I CGWCG 3 cut(s) 731, 1290, 2099
HpyCH4III ACNGT 7 cut(s) 1004, 2037, 2163, 2363, 2471, 2546, 3139
HpyCH4IV ACGT 4 cut(s) 2173, 2581, 2925, 2959
HpyF10VI GCNNNNNNNGC 3 cut(s) 641, 1229, 2355
HpyF3I CTNAG 9 cut(s) 308, 432, 1856, 1988, 2009, 2048, 2487, 2897, 3039
HpySE526I ACGT 4 cut(s) 2173, 2581, 2925, 2959
Hsp92I GRCGYC 1 cut(s) 726
HspAI GCGC 3 cut(s) 677, 862, 1206
Kpn2I TCCGGA 1 cut(s) 2452
Ksp22I TGATCA 1 cut(s) 661
KspI CCGCGG 1 cut(s) 2734
Kzo9I GATC 8 cut(s) 382, 661, 936, 1792, 1806, 1852, 2723, 2756
LmnI GCTCC 4 cut(s) 70, 1122, 2959, 3278
Lsp1109I GCAGC 6 cut(s) 472, 484, 846, 1267, 1391, 2367
MaeI CTAG 8 cut(s) 1197, 1493, 1542, 1560, 2168, 2304, 2495, 2921
MaeII ACGT 4 cut(s) 2173, 2581, 2925, 2959
MaeIII GTNAC 3 cut(s) 2522, 2712, 2765
MalI GATC 8 cut(s) 384, 663, 938, 1794, 1808, 1854, 2725, 2758
MboI GATC 8 cut(s) 382, 661, 936, 1792, 1806, 1852, 2723, 2756
MfeI CAATTG 1 cut(s) 1434
MflI RGATCY 5 cut(s) 382, 936, 1806, 1852, 2723
MhlI GDGCHC 4 cut(s) 901, 1246, 2390, 3254
MlsI TGGCCA 2 cut(s) 1675, 2990
MluNI TGGCCA 2 cut(s) 1675, 2990
MlyI GAGTC 4 cut(s) 717, 1307, 1808, 1854
MmeI TCCRAC 1 cut(s) 3069
Mox20I TGGCCA 2 cut(s) 1675, 2990
Mph1103I ATGCAT 3 cut(s) 329, 1346, 1587
MroI TCCGGA 1 cut(s) 2452
MscI TGGCCA 2 cut(s) 1675, 2990
MseI TTAA 9 cut(s) 237, 491, 1352, 1533, 1841, 1971, 2261, 2555, 3161
MslI CAYNNNNRTG 3 cut(s) 318, 2531, 2580
Msp20I TGGCCA 2 cut(s) 1675, 2990
MspA1I CMGCKG 2 cut(s) 1636, 2733
MspI CCGG 1 cut(s) 2453
MspR9I CCNGG 3 cut(s) 2721, 2972, 3011
MunI CAATTG 1 cut(s) 1434
Mva1269I GAATGC 4 cut(s) 329, 679, 1208, 2620
MvaI CCWGG 3 cut(s) 2721, 2972, 3011
MvnI CGCG 2 cut(s) 1208, 2733
MwoI GCNNNNNNNGC 3 cut(s) 641, 1229, 2355
NcoI CCATGG 1 cut(s) 1068
NdeII GATC 8 cut(s) 382, 661, 936, 1792, 1806, 1852, 2723, 2756
NlaIV GGNNCC 4 cut(s) 384, 1244, 2446, 3305
NsiI ATGCAT 3 cut(s) 329, 1346, 1587
NspI RCATGY 1 cut(s) 2628
OliI CACNNNNGTG 1 cut(s) 318
PagI TCATGA 2 cut(s) 16, 669
PciI ACATGT 1 cut(s) 2624
PcsI WCGNNNNNNNCGW 1 cut(s) 2091
PctI GAATGC 4 cut(s) 329, 679, 1208, 2620
PfeI GAWTC 9 cut(s) 20, 165, 242, 1115, 1379, 1481, 1538, 1707, 1984
PflMI CCANNNNNTGG 1 cut(s) 1750
PkrI GCNGC 7 cut(s) 474, 487, 861, 1282, 1406, 1862, 2357
PleI GAGTC 4 cut(s) 716, 1306, 1808, 1853
PmaCI CACGTG 1 cut(s) 2960
PmlI CACGTG 1 cut(s) 2960
PpsI GAGTC 4 cut(s) 716, 1306, 1808, 1853
Ppu21I YACGTR 2 cut(s) 2582, 2960
PscI ACATGT 1 cut(s) 2624
PshAI GACNNNNGTC 1 cut(s) 2424
PsiI TTATAA 2 cut(s) 534, 2409
Psp124BI GAGCTC 1 cut(s) 2390
Psp6I CCWGG 3 cut(s) 2719, 2970, 3009
PspCI CACGTG 1 cut(s) 2960
PspGI CCWGG 3 cut(s) 2719, 2970, 3009
PspN4I GGNNCC 4 cut(s) 384, 1244, 2446, 3305
PspOMI GGGCCC 1 cut(s) 1242
PspPI GGNCC 3 cut(s) 1242, 1243, 1528
PsuI RGATCY 5 cut(s) 382, 936, 1806, 1852, 2723
RsaI GTAC 8 cut(s) 396, 762, 1337, 2068, 2091, 2165, 2580, 3188
RsaNI GTAC 8 cut(s) 395, 761, 1336, 2067, 2090, 2164, 2579, 3187
RseI CAYNNNNRTG 3 cut(s) 318, 2531, 2580
SacI GAGCTC 1 cut(s) 2390
SacII CCGCGG 1 cut(s) 2734
SaqAI TTAA 9 cut(s) 237, 491, 1352, 1533, 1841, 1971, 2261, 2555, 3161
SatI GCNGC 7 cut(s) 473, 486, 860, 1281, 1405, 1861, 2356
Sau3AI GATC 8 cut(s) 382, 661, 936, 1792, 1806, 1852, 2723, 2756
Sau96I GGNCC 3 cut(s) 1242, 1243, 1528
ScaI AGTACT 1 cut(s) 2068
SchI GAGTC 4 cut(s) 717, 1307, 1808, 1854
ScrFI CCNGG 3 cut(s) 2721, 2972, 3011
SduI GDGCHC 4 cut(s) 901, 1246, 2390, 3254
SfcI CTRYAG 1 cut(s) 1179
Sfr303I CCGCGG 1 cut(s) 2734
SgrBI CCGCGG 1 cut(s) 2734
SmiMI CAYNNNNRTG 3 cut(s) 318, 2531, 2580
SmlI CTYRAG 3 cut(s) 602, 872, 1979
SmoI CTYRAG 3 cut(s) 602, 872, 1979
SsiI CCGC 6 cut(s) 1208, 1636, 1860, 2112, 2731, 2733
SspI AATATT 2 cut(s) 2227, 3150
SspMI CTAG 8 cut(s) 1197, 1493, 1542, 1560, 2168, 2304, 2495, 2921
SstI GAGCTC 1 cut(s) 2390
StyD4I CCNGG 3 cut(s) 2719, 2970, 3009
StyI CCWWGG 1 cut(s) 1068
TaaI ACNGT 7 cut(s) 1004, 2037, 2163, 2363, 2471, 2546, 3139
TaiI ACGT 4 cut(s) 2176, 2584, 2928, 2962
TaqI TCGA 8 cut(s) 115, 145, 1249, 1285, 2319, 2340, 3102, 3324
TatI WGTACW 3 cut(s) 760, 1335, 2066
TauI GCSGC 1 cut(s) 1863
TfiI GAWTC 9 cut(s) 20, 165, 242, 1115, 1379, 1481, 1538, 1707, 1984
Tru1I TTAA 9 cut(s) 237, 491, 1352, 1533, 1841, 1971, 2261, 2555, 3161
Tru9I TTAA 9 cut(s) 237, 491, 1352, 1533, 1841, 1971, 2261, 2555, 3161
TscAI CASTG 6 cut(s) 482, 1708, 1870, 2166, 2368, 3047
TseI GCWGC 6 cut(s) 472, 485, 859, 1280, 1404, 2355
TspGWI ACGGA 2 cut(s) 471, 729
TspRI CASTG 6 cut(s) 482, 1708, 1870, 2166, 2368, 3047
Van91I CCANNNNNTGG 1 cut(s) 1750
XapI RAATTY 6 cut(s) 357, 505, 569, 834, 990, 1728
XbaI TCTAGA 1 cut(s) 2920
XceI RCATGY 1 cut(s) 2628
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 711
XspI CTAG 8 cut(s) 1197, 1493, 1542, 1560, 2168, 2304, 2495, 2921
ZrmI AGTACT 1 cut(s) 2068
Zsp2I ATGCAT 3 cut(s) 329, 1346, 1587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.