MD12G1107600.v1.1

RING finger and CHY zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005998
Physical Location & Seq
Forward (+)
16979907 .. 16982239
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1107600.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGACGGCGGATTCGAGCCCCAGGTTTCCATTGACGACGACCATTCTCACCAGAAAGTCAACTCTCTCACCGAGATGGTATCTGGGAATTTCGGGTGCGCGCATTACAGACGGAGGTGTAAGATCAGAGCACCCTGCTGTGATCAGATATTCGATTGCAGGCATTGCCATAACGAAGCTAAGAATTCATTGGAAGTTGATCCTCTTGATCGCCATGACGTTCCTCGCCACGATGTGAAAAGGATCATCTGCTCCTTGTGCAACAAAGAACAAGATGTTCAACAGCTCTGTATCCAATGTGGGGTTTGCATGGGAAACTATTTTTGCTCCAAATGCAAGTTCTTTGACGATGACGTTTCGAAGAAACAGTATCATTGTGATGAATGCGGTATCTGCAGAACTGGAGGTGGGGAGAATTTTTTCCACTGTAACAAATGTGGATGTTGTTACTCAAATGCGTTGAAGGATTCACACAATTGTGTGGAAAAAGCAATGCATCATAATTGCCCTGTGTGCTTTGAGGTAAAGTTTGGAGGATACCATGTCCAAGTATTAAGGGAAATTTGGAAAGTTAACATTCTTTCTAACAAACTAATGACTTTTAGCCTCTTTTTCAAGATTTGGATATATATCGAGGCTCCCTCGAGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0036211 GO:0042176 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051865 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
ko04115 ko04120 ko05162 map04115 map04120 map05162
Pfam Domains
Protein Families
zf-CHY zf-RING_2 zf-RING_UBOX zinc_ribbon_6

Protein Analysis

218

Amino Acids

24.91

Weight (kDa)

6.86

Isoelectric Point (pI)

50.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CHY PF05495 33 - 107 3.4e-19 CHY zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 101
AciI CCGC 2 cut(s) 9, 387
AclWI GGATC 2 cut(s) 194, 251
AcsI RAATTY 4 cut(s) 88, 184, 415, 561
AdeI CACNNNGTG 1 cut(s) 235
AfiI CCNNNNNNNGG 2 cut(s) 301, 647
AgsI TTSAA 3 cut(s) 281, 463, 616
AjnI CCWGG 1 cut(s) 21
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AleI CACNNNNGTG 1 cut(s) 477
AluBI AGCT 2 cut(s) 179, 286
AluI AGCT 2 cut(s) 179, 286
Alw21I GWGCWC 1 cut(s) 133
AlwI GGATC 2 cut(s) 194, 251
Ama87I CYCGRG 1 cut(s) 643
ApoI RAATTY 4 cut(s) 88, 184, 415, 561
ArsI GACNNNNNNTTYG 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 2 cut(s) 101, 103
AsuHPI GGTGA 2 cut(s) 41, 61
AsuII TTCGAA 1 cut(s) 359
AvaI CYCGRG 1 cut(s) 643
BanII GRGCYC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 133
BccI CCATC 1 cut(s) 70
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 23
BciVI GTATCC 2 cut(s) 302, 530
BclI TGATCA 1 cut(s) 142
BfmI CTRYAG 1 cut(s) 394
BfuI GTATCC 2 cut(s) 302, 530
Bme1390I CCNGG 1 cut(s) 23
BmeT110I CYCGRG 1 cut(s) 643
BmiI GGNNCC 1 cut(s) 639
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 1 cut(s) 505
BplI GAGNNNNNCTC 1 cut(s) 626
BpmI CTGGAG 1 cut(s) 423
Bpu14I TTCGAA 1 cut(s) 359
BsaBI GATNNNNATC 1 cut(s) 629
BsaJI CCNNGG 1 cut(s) 21
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 647
Bse1I ACTGG 1 cut(s) 406
Bse3DI GCAATG 2 cut(s) 163, 498
Bse8I GATNNNNATC 1 cut(s) 629
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 1 cut(s) 446
BseJI GATNNNNATC 1 cut(s) 629
BseLI CCNNNNNNNGG 2 cut(s) 301, 647
BseMI GCAATG 2 cut(s) 163, 498
BseNI ACTGG 1 cut(s) 406
BsePI GCGCGC 1 cut(s) 99
Bsh1236I CGCG 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 643
BslI CCNNNNNNNGG 2 cut(s) 301, 647
BsmI GAATGC 1 cut(s) 389
BsoBI CYCGRG 1 cut(s) 643
Bsp119I TTCGAA 1 cut(s) 359
Bsp1286I GDGCHC 2 cut(s) 21, 133
Bsp143I GATC 5 cut(s) 123, 142, 199, 208, 243
BspACI CCGC 2 cut(s) 9, 387
BspFNI CGCG 1 cut(s) 101
BspLI GGNNCC 1 cut(s) 639
BspMAI CTGCAG 1 cut(s) 398
BspPI GGATC 2 cut(s) 194, 251
BspT104I TTCGAA 1 cut(s) 359
BsrDI GCAATG 2 cut(s) 163, 498
BsrI ACTGG 1 cut(s) 406
BssECI CCNNGG 1 cut(s) 21
BssHII GCGCGC 1 cut(s) 99
BssMI GATC 5 cut(s) 123, 142, 199, 208, 243
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 369, 428
BstAPI GCANNNNNTGC 1 cut(s) 165
BstBI TTCGAA 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 101, 161
BstDEI CTNAG 1 cut(s) 180
BstF5I GGATG 1 cut(s) 446
BstFNI CGCG 1 cut(s) 101
BstHHI GCGC 2 cut(s) 101, 103
BstKTI GATC 5 cut(s) 126, 145, 202, 211, 246
BstMBI GATC 5 cut(s) 123, 142, 199, 208, 243
BstMWI GCNNNNNNNGC 5 cut(s) 165, 258, 333, 393, 513
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 394
BstUI CGCG 1 cut(s) 101
BsuI GTATCC 2 cut(s) 302, 530
BtsCI GGATG 1 cut(s) 446
BtsIMutI CAGTG 1 cut(s) 424
Cac8I GCNNGC 2 cut(s) 101, 161
CfoI GCGC 2 cut(s) 101, 103
CviAII CATG 3 cut(s) 215, 310, 542
CviJI RGCY 5 cut(s) 19, 179, 286, 606, 638
CviKI_1 RGCY 5 cut(s) 19, 179, 286, 606, 638
DdeI CTNAG 1 cut(s) 180
DpnI GATC 5 cut(s) 125, 144, 201, 210, 245
DpnII GATC 5 cut(s) 123, 142, 199, 208, 243
DraIII CACNNNGTG 1 cut(s) 235
EciI GGCGGA 1 cut(s) 24
Eco24I GRGCYC 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 643
EcoRI GAATTC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 21
FaeI CATG 3 cut(s) 218, 313, 545
FaiI YATR 7 cut(s) 171, 216, 311, 501, 543, 628, 630
FatI CATG 3 cut(s) 214, 309, 541
FbaI TGATCA 1 cut(s) 142
FokI GGATG 1 cut(s) 453
FriOI GRGCYC 1 cut(s) 21
GlaI GCGC 2 cut(s) 100, 102
GsuI CTGGAG 1 cut(s) 423
HhaI GCGC 2 cut(s) 101, 103
Hin1II CATG 3 cut(s) 218, 313, 545
Hin6I GCGC 2 cut(s) 99, 101
HinP1I GCGC 2 cut(s) 99, 101
HincII GTYRAC 2 cut(s) 61, 574
HindII GTYRAC 2 cut(s) 61, 574
HinfI GANTC 2 cut(s) 12, 467
HpaI GTTAAC 1 cut(s) 574
HphI GGTGA 2 cut(s) 41, 61
Hpy166II GTNNAC 2 cut(s) 61, 574
Hpy188I TCNGA 2 cut(s) 128, 147
Hpy188III TCNNGA 2 cut(s) 206, 616
Hpy8I GTNNAC 2 cut(s) 61, 574
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 2 cut(s) 369, 428
HpyCH4IV ACGT 2 cut(s) 219, 354
HpyCH4V TGCA 6 cut(s) 159, 261, 309, 336, 396, 496
HpyF10VI GCNNNNNNNGC 5 cut(s) 165, 258, 333, 393, 513
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 2 cut(s) 219, 354
Hsp92II CATG 3 cut(s) 218, 313, 545
HspAI GCGC 2 cut(s) 99, 101
Ksp22I TGATCA 1 cut(s) 142
KspAI GTTAAC 1 cut(s) 574
Kzo9I GATC 5 cut(s) 123, 142, 199, 208, 243
LmnI GCTCC 3 cut(s) 257, 332, 643
LpnPI CCDG 8 cut(s) 8, 35, 65, 69, 145, 148, 387, 522
LweI GCATC 1 cut(s) 505
MaeII ACGT 2 cut(s) 219, 354
MaeIII GTNAC 2 cut(s) 428, 446
MalI GATC 5 cut(s) 125, 144, 201, 210, 245
MboI GATC 5 cut(s) 123, 142, 199, 208, 243
MboII GAAGA 1 cut(s) 373
MfeI CAATTG 1 cut(s) 475
MhlI GDGCHC 2 cut(s) 21, 133
MluCI AATT 6 cut(s) 88, 184, 415, 475, 502, 561
MnlI CCTC 9 cut(s) 108, 213, 234, 398, 514, 527, 617, 628, 652
Mph1103I ATGCAT 1 cut(s) 498
MroXI GAANNNNTTC 1 cut(s) 419
MseI TTAA 3 cut(s) 554, 573, 652
MslI CAYNNNNRTG 3 cut(s) 74, 378, 477
MspR9I CCNGG 1 cut(s) 23
MunI CAATTG 1 cut(s) 475
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 5 cut(s) 165, 258, 333, 393, 513
NdeII GATC 5 cut(s) 123, 142, 199, 208, 243
NlaIII CATG 3 cut(s) 218, 313, 545
NlaIV GGNNCC 1 cut(s) 639
NsiI ATGCAT 1 cut(s) 498
NspV TTCGAA 1 cut(s) 359
OliI CACNNNNGTG 1 cut(s) 477
PaeR7I CTCGAG 1 cut(s) 643
PauI GCGCGC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PctI GAATGC 1 cut(s) 389
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 2 cut(s) 12, 467
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 639
PspXI VCTCGAGB 1 cut(s) 643
PstI CTGCAG 1 cut(s) 398
PteI GCGCGC 1 cut(s) 99
RseI CAYNNNNRTG 3 cut(s) 74, 378, 477
SaqAI TTAA 3 cut(s) 554, 573, 652
Sau3AI GATC 5 cut(s) 123, 142, 199, 208, 243
ScrFI CCNGG 1 cut(s) 23
SduI GDGCHC 2 cut(s) 21, 133
SetI ASST 8 cut(s) 27, 119, 181, 222, 288, 357, 409, 525
SfaNI GCATC 1 cut(s) 505
SfcI CTRYAG 1 cut(s) 394
Sfr274I CTCGAG 1 cut(s) 643
SfuI TTCGAA 1 cut(s) 359
SlaI CTCGAG 1 cut(s) 643
SmiMI CAYNNNNRTG 3 cut(s) 74, 378, 477
SmlI CTYRAG 1 cut(s) 643
SmoI CTYRAG 1 cut(s) 643
Sse9I AATT 6 cut(s) 88, 184, 415, 475, 502, 561
SsiI CCGC 2 cut(s) 9, 387
StyD4I CCNGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 369, 428
TaiI ACGT 2 cut(s) 222, 357
TaqI TCGA 5 cut(s) 15, 153, 359, 633, 644
TasI AATT 6 cut(s) 88, 184, 415, 475, 502, 561
TfiI GAWTC 2 cut(s) 12, 467
Tru1I TTAA 3 cut(s) 554, 573, 652
Tru9I TTAA 3 cut(s) 554, 573, 652
TscAI CASTG 1 cut(s) 431
TspDTI ATGAA 2 cut(s) 177, 396
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 1 cut(s) 431
XapI RAATTY 4 cut(s) 88, 184, 415, 561
XhoI CTCGAG 1 cut(s) 643
XmnI GAANNNNTTC 1 cut(s) 419
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.