Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0005998

Physical Location & Seq

Genomic Coordinates Reverse (-)

35356597 .. 35360606

Gene Structure 4010 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0303400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGCTATCAGTCACTCAACCTCTCCTTCTCCACAGAAACTCCACCCTCTTCTTCTTCCCATCATTCCCAATCCGCACGAGGCCTCCGAGCCCCATCAGGATCTCACGATGCTCTTCGGGCCTCCAAGAGGCCAAGGACGCAGATCAGCTCGGATCGGACGAGCTCTCCGCCTCGTCGCCGTTCCCGGCCGTCAAGGGGGCGAAGAGAGTGGTTCTGGTGAGGCACGGGCAGAGCACGTGGAACGAGGAAGGTAGGATCCAAGGCAGCTCCAATTTCTCAGTGCTCACCAAGAAAGGCGAGGCTCAGGCCCAGACTTCCCGCCAAATGCTCATCGGAGATGCCTTCGATGTCTGCTTCGCCAGTCCCTTGGCACGGTCAAAGAGAACAGCCGAAATTATATGGGACACTCGCAAAGAGGAAATGATTATGGATTTTGATTTGAGGGAAATCGACTTGTACTCATTTCAAGGCCTCTTAAAGCATGAGGGAAAAGAAAAGTTTGGTGCAGCTTTTCAGAAATGGCAGGTAGATGCACCAAATTTTAGCATTGATGATCATTATCCAGTGAGAGAGTTATGGGCACGTGCTAGGAGCTGCTGGACCAGAATACTATTGCATGAAAGCAGGTCTGTTCTTGTGGTTGCTCATAATGCTGTTAATCAGGCTCTCCTTGCAACAGCCATTGGACTGGGAACAGAGTATTTCAGGACTTTAGTTCAGAGCAATTGTGGTGTAAGCGTACTGGACTTCACCCCACGTGCTGAGGGTGGACCACCATATATCTGCCTTAATCGTCTAAACCAGACCCCAAGTTCACCTATTGAAGCTGGAAGTTCAGGGGGAAGGAAAACTAGTAAGCGGATCATACTTGTTTGTCACGGATCCACACAAATGAATGCAGAGCCAATGGACATGCTTGGGGTTATAAAGGCCCAGAAAATTGCCGAGCTACTTCTTGATTTAAAAGTGAACTCTATAGTTAGCAGTCCTAAGGAAGCTTGTGTTGAAACAGCGATTGCCATCTCCAGAGTACAAGAAGCTGCAGATTGTTTGGGAGCTGACTCTGTGCCACGCTCTGTGGAGATGAAGCAGATGGAGAACCTTGATGTTGAAAACATTCTTCAGAAGTCAAATAAGAATGAAATGGAGATTCCACCTCTTCAGCCCGGTTGTCTTAATGGATTTGAGGATAGAGTCGTGGAAGCTGTGTGGGATCAATCTGAGAAGGCCTGGCAGTCTCTGCTAGAAGAAGTAGCTGATGAAGCAGCACCGGAAAAAGTTGTTGTTGCAGTTGGTCATCCTGCATTGCACATAGCATTGCTGGGGCGCTGCCTGAATTTGACAAAAGAATGGTTGGGATCATTTCATCTTGATGCAGGAAGCATCAGTATCATTGATTTTCCAGATGGTCCTAGTGGGAGAGGTGTTATTCGGTGCATAAACTATACTGCCCATTTGGGGAGATGGTCAGTACCTATAACCAAATCAACAGTAAACGATAAAAATATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0010119 GO:0050789 GO:0050794 GO:0065007 GO:1902456

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko05162 map04115 map04120 map05162

Pfam Domains

Protein Families

zf-CHY zf-RING_2 zf-RING_UBOX zinc_ribbon_6

Protein Analysis

503

Amino Acids

55.44

Weight (kDa)

6.3

Isoelectric Point (pI)

50.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
His_Phos_1	PF00300	70 - 253	3e-40	Histidine phosphatase superfamily (branch 1)
His_Phos_1	PF00300	288 - 470	7.4e-11	Histidine phosphatase superfamily (branch 1)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005998)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22920 AT5G22920
fragaria_vesca	FvH4_6g16750
malus_domestica	MD12G1107600.v1.1
prunus_persica	Prupe.7G126100_v2.0.a1
pyrus_communis	pycom04g08360 pycom12g10770
rosa_chinensis	RchiOBHm_Chr3g0470691 RchiOBHm_Chr5g0056891
rosa_laevigata	RLG00000024247
rosa_multiflora	Rmu_sc0006168.1_g000024
rosa_roxburghii	Rroxscaffold_1G00023410 Rroxscaffold_6G00410580
rosa_rugosa	Rorug03G0112100 Rorug03G0112200 Rorug05G0303400 Rorug05G0303500
rosa_samantha	Rh3AG161700 Rh3BG186200 Rh3CG176600 Rh3DG188900 Rh5AG373400 Rh5CG407600 Rh5DG397700
rosa_wichuraiana	Rw0G004620 Rw3G015250 Rw5G035110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	926
Acc36I	ACCTGC	2 cut(s)	514, 615
AciI	CCGC	4 cut(s)	73, 168, 319, 859
AclWI	GGATC	9 cut(s)	107, 160, 250, 263, 869, 876, 889, 1221, 1366
AcoI	YGGCCR	1 cut(s)	186
AcsI	RAATTY	2 cut(s)	538, 1336
AcuI	CTGAAG	2 cut(s)	1106, 1145
AcvI	CACGTG	3 cut(s)	237, 584, 758
AfaI	GTAC	4 cut(s)	458, 741, 1032, 1473
AfiI	CCNNNNNNNGG	4 cut(s)	127, 195, 372, 1458
AgsI	TTSAA	4 cut(s)	467, 824, 1007, 1112
AhlI	ACTAGT	1 cut(s)	851
AjnI	CCWGG	1 cut(s)	1229
Alw21I	GWGCWC	3 cut(s)	165, 236, 285
Alw26I	GTCTC	1 cut(s)	1242
AlwI	GGATC	9 cut(s)	107, 160, 250, 263, 869, 876, 889, 1221, 1366
AlwNI	CAGNNNCTG	1 cut(s)	1240
AoxI	GGCC	8 cut(s)	80, 118, 129, 186, 306, 469, 930, 1227
ApeKI	GCWGC	6 cut(s)	264, 506, 594, 1040, 1265, 1329
ApoI	RAATTY	2 cut(s)	538, 1336
Asp700I	GAANNNNTTC	1 cut(s)	1116
AspLEI	GCGC	1 cut(s)	1329
AspS9I	GGNCC	6 cut(s)	118, 307, 600, 770, 931, 1409
AsuC2I	CCSGG	2 cut(s)	185, 1167
AsuHPI	GGTGA	4 cut(s)	229, 277, 742, 807
AvaII	GGWCC	3 cut(s)	600, 770, 1409
AxyI	CCTNAGG	1 cut(s)	990
BaeGI	GKGCMC	1 cut(s)	583
BamHI	GGATCC	2 cut(s)	255, 881
BanII	GRGCYC	2 cut(s)	92, 165
BauI	CACGAG	1 cut(s)	76
BbrPI	CACGTG	3 cut(s)	237, 584, 758
Bbv12I	GWGCWC	3 cut(s)	165, 236, 285
BbvCI	CCTCAGC	1 cut(s)	762
BbvI	GCAGC	6 cut(s)	276, 518, 581, 1027, 1277, 1316
BccI	CCATC	6 cut(s)	67, 101, 1028, 1087, 1400, 1458
BceAI	ACGGC	2 cut(s)	163, 173
BciT130I	CCWGG	1 cut(s)	1231
BclI	TGATCA	1 cut(s)	553
BcnI	CCSGG	2 cut(s)	185, 1167
BcoDI	GTCTC	1 cut(s)	1242
BcuI	ACTAGT	1 cut(s)	851
BfaI	CTAG	4 cut(s)	588, 852, 1244, 1413
BfmI	CTRYAG	2 cut(s)	975, 1041
BfoI	RGCGCY	1 cut(s)	1330
BfuAI	ACCTGC	2 cut(s)	514, 615
BisI	GCNGC	6 cut(s)	265, 507, 595, 1041, 1266, 1330
BlsI	GCNGC	6 cut(s)	266, 508, 596, 1042, 1267, 1331
Bme1390I	CCNGG	3 cut(s)	185, 1167, 1231
Bme18I	GGWCC	3 cut(s)	600, 770, 1409
BmgT120I	GGNCC	6 cut(s)	118, 307, 600, 770, 931, 1409
BmiI	GGNNCC	2 cut(s)	257, 883
BmrFI	CCNGG	3 cut(s)	185, 1167, 1231
BmrI	ACTGGG	1 cut(s)	698
BmsI	GCATC	5 cut(s)	98, 328, 520, 1363, 1392
BmuI	ACTGGG	1 cut(s)	698
BpmI	CTGGAG	1 cut(s)	1009
Bpu10I	CCTNAGC	2 cut(s)	303, 762
BpuMI	CCSGG	2 cut(s)	185, 1167
BsaAI	YACGTR	3 cut(s)	237, 584, 758
BsaBI	GATNNNNATC	2 cut(s)	558, 1019
BsaJI	CCNNGG	3 cut(s)	132, 259, 366
BsaWI	WCCGGW	1 cut(s)	1270
BsaXI	ACNNNNNCTCC	6 cut(s)	23, 53, 67, 97, 149, 179
Bsc4I	CCNNNNNNNGG	4 cut(s)	127, 195, 372, 1458
Bse1I	ACTGG	4 cut(s)	360, 563, 693, 747
Bse21I	CCTNAGG	1 cut(s)	990
Bse3DI	GCAATG	2 cut(s)	1304, 1316
Bse8I	GATNNNNATC	2 cut(s)	558, 1019
BseBI	CCWGG	1 cut(s)	1231
BseDI	CCNNGG	3 cut(s)	132, 259, 366
BseGI	GGATG	1 cut(s)	1297
BseJI	GATNNNNATC	2 cut(s)	558, 1019
BseLI	CCNNNNNNNGG	4 cut(s)	127, 195, 372, 1458
BseMI	GCAATG	2 cut(s)	1304, 1316
BseMII	CTCAG	4 cut(s)	291, 317, 753, 1212
BseNI	ACTGG	4 cut(s)	360, 563, 693, 747
BseSI	GKGCMC	1 cut(s)	583
BseX3I	CGGCCG	1 cut(s)	186
BseXI	GCAGC	6 cut(s)	276, 518, 581, 1027, 1277, 1316
BseYI	CCCAGC	1 cut(s)	1321
BsgI	GTGCAG	1 cut(s)	525
Bsh1285I	CGRYCG	1 cut(s)	189
BshFI	GGCC	8 cut(s)	82, 120, 131, 188, 308, 471, 932, 1229
BsiEI	CGRYCG	1 cut(s)	189
BsiHKAI	GWGCWC	3 cut(s)	165, 236, 285
BsiSI	CCGG	3 cut(s)	185, 1167, 1271
BslFI	GGGAC	2 cut(s)	348, 416
BslI	CCNNNNNNNGG	4 cut(s)	127, 195, 372, 1458
BsmAI	GTCTC	1 cut(s)	1242
BsmFI	GGGAC	2 cut(s)	348, 416
BsmI	GAATGC	1 cut(s)	901
BsnI	GGCC	8 cut(s)	82, 120, 131, 188, 308, 471, 932, 1229
Bsp1286I	GDGCHC	5 cut(s)	92, 165, 236, 285, 583
Bsp143I	GATC	9 cut(s)	99, 142, 152, 255, 553, 861, 881, 1213, 1358
BspACI	CCGC	4 cut(s)	73, 168, 319, 859
BspANI	GGCC	8 cut(s)	82, 120, 131, 188, 308, 471, 932, 1229
BspCNI	CTCAG	4 cut(s)	290, 316, 754, 1213
BspLI	GGNNCC	2 cut(s)	257, 883
BspMAI	CTGCAG	1 cut(s)	1045
BspMI	ACCTGC	2 cut(s)	514, 615
BspPI	GGATC	9 cut(s)	107, 160, 250, 263, 869, 876, 889, 1221, 1366
BspQI	GCTCTTC	1 cut(s)	118
BsrDI	GCAATG	2 cut(s)	1304, 1316
BsrI	ACTGG	4 cut(s)	360, 563, 693, 747
BssECI	CCNNGG	3 cut(s)	132, 259, 366
BssMI	GATC	9 cut(s)	99, 142, 152, 255, 553, 861, 881, 1213, 1358
BssSI	CACGAG	1 cut(s)	76
BssT1I	CCWWGG	3 cut(s)	132, 259, 366
Bst2BI	CACGAG	1 cut(s)	76
Bst2UI	CCWGG	1 cut(s)	1231
Bst4CI	ACNGT	2 cut(s)	375, 1492
Bst6I	CTCTTC	4 cut(s)	53, 118, 197, 1164
BstAPI	GCANNNNNTGC	1 cut(s)	1240
BstBAI	YACGTR	3 cut(s)	237, 584, 758
BstDEI	CTNAG	5 cut(s)	277, 303, 762, 990, 1221
BstENI	CCTNNNNNAGG	1 cut(s)	125
BstF5I	GGATG	1 cut(s)	1297
BstH2I	RGCGCY	1 cut(s)	1330
BstHHI	GCGC	1 cut(s)	1329
BstKTI	GATC	9 cut(s)	102, 145, 155, 258, 556, 864, 884, 1216, 1361
BstMAI	GTCTC	1 cut(s)	1242
BstMBI	GATC	9 cut(s)	99, 142, 152, 255, 553, 861, 881, 1213, 1358
BstMCI	CGRYCG	1 cut(s)	189
BstMWI	GCNNNNNNNGC	6 cut(s)	117, 137, 650, 671, 1240, 1262
BstNI	CCWGG	1 cut(s)	1231
BstNSI	RCATGY	1 cut(s)	916
BstSCI	CCNGG	3 cut(s)	183, 1165, 1229
BstSFI	CTRYAG	2 cut(s)	975, 1041
BstSLI	GKGCMC	1 cut(s)	583
BstV1I	GCAGC	6 cut(s)	276, 518, 581, 1027, 1277, 1316
BstX2I	RGATCY	3 cut(s)	99, 255, 881
BstXI	CCANNNNNNTGG	2 cut(s)	367, 688
BstYI	RGATCY	3 cut(s)	99, 255, 881
BstZI	CGGCCG	1 cut(s)	186
Bsu36I	CCTNAGG	1 cut(s)	990
BsuRI	GGCC	8 cut(s)	82, 120, 131, 188, 308, 471, 932, 1229
BtsCI	GGATG	1 cut(s)	1297
BtsIMutI	CAGTG	2 cut(s)	285, 570
BveI	ACCTGC	2 cut(s)	514, 615
CaiI	CAGNNNCTG	1 cut(s)	1240
CfoI	GCGC	1 cut(s)	1329
Cfr13I	GGNCC	6 cut(s)	118, 307, 600, 770, 931, 1409
CseI	GACGC	1 cut(s)	146
Csp6I	GTAC	4 cut(s)	457, 740, 1031, 1472
CviAII	CATG	3 cut(s)	482, 617, 913
CviQI	GTAC	4 cut(s)	457, 740, 1031, 1472
DdeI	CTNAG	5 cut(s)	277, 303, 762, 990, 1221
DpnI	GATC	9 cut(s)	101, 144, 154, 257, 555, 863, 883, 1215, 1360
DpnII	GATC	9 cut(s)	99, 142, 152, 255, 553, 861, 881, 1213, 1358
DraI	TTTAAA	1 cut(s)	963
EaeI	YGGCCR	1 cut(s)	186
EagI	CGGCCG	1 cut(s)	186
Eam1104I	CTCTTC	4 cut(s)	53, 118, 197, 1164
EarI	CTCTTC	4 cut(s)	53, 118, 197, 1164
EciI	GGCGGA	1 cut(s)	157
Ecl136II	GAGCTC	1 cut(s)	163
EclXI	CGGCCG	1 cut(s)	186
Eco130I	CCWWGG	3 cut(s)	132, 259, 366
Eco147I	AGGCCT	3 cut(s)	82, 471, 1229
Eco24I	GRGCYC	2 cut(s)	92, 165
Eco47I	GGWCC	3 cut(s)	600, 770, 1409
Eco52I	CGGCCG	1 cut(s)	186
Eco53kI	GAGCTC	1 cut(s)	163
Eco57I	CTGAAG	2 cut(s)	1106, 1145
Eco72I	CACGTG	3 cut(s)	237, 584, 758
Eco81I	CCTNAGG	1 cut(s)	990
EcoICRI	GAGCTC	1 cut(s)	163
EcoNI	CCTNNNNNAGG	1 cut(s)	125
EcoRII	CCWGG	1 cut(s)	1229
EcoT14I	CCWWGG	3 cut(s)	132, 259, 366
EcoT38I	GRGCYC	2 cut(s)	92, 165
ErhI	CCWWGG	3 cut(s)	132, 259, 366
FaeI	CATG	3 cut(s)	485, 620, 916
FaqI	GGGAC	2 cut(s)	348, 416
FatI	CATG	3 cut(s)	481, 616, 912
FauI	CCCGC	1 cut(s)	326
FbaI	TGATCA	1 cut(s)	553
Fnu4HI	GCNGC	6 cut(s)	265, 507, 595, 1041, 1266, 1330
FokI	GGATG	1 cut(s)	1284
FriOI	GRGCYC	2 cut(s)	92, 165
Fsp4HI	GCNGC	6 cut(s)	265, 507, 595, 1041, 1266, 1330
FspBI	CTAG	4 cut(s)	588, 852, 1244, 1413
GlaI	GCGC	1 cut(s)	1328
GluI	GCNGC	6 cut(s)	265, 507, 595, 1041, 1266, 1330
GsaI	CCCAGC	1 cut(s)	1325
GsuI	CTGGAG	1 cut(s)	1009
HaeII	RGCGCY	1 cut(s)	1330
HaeIII	GGCC	8 cut(s)	82, 120, 131, 188, 308, 471, 932, 1229
HapII	CCGG	3 cut(s)	185, 1167, 1271
HgaI	GACGC	1 cut(s)	146
HhaI	GCGC	1 cut(s)	1329
Hin1II	CATG	3 cut(s)	485, 620, 916
Hin6I	GCGC	1 cut(s)	1327
HinP1I	GCGC	1 cut(s)	1327
HindIII	AAGCTT	1 cut(s)	996
HinfI	GANTC	3 cut(s)	1061, 1150, 1194
HpaII	CCGG	3 cut(s)	185, 1167, 1271
HphI	GGTGA	4 cut(s)	229, 277, 742, 807
Hpy166II	GTNNAC	4 cut(s)	770, 815, 970, 1495
Hpy188I	TCNGA	8 cut(s)	87, 152, 157, 335, 516, 720, 1125, 1222
Hpy188III	TCNNGA	7 cut(s)	97, 105, 706, 956, 1026, 1370, 1403
Hpy8I	GTNNAC	4 cut(s)	770, 815, 970, 1495
Hpy99I	CGWCG	1 cut(s)	178
HpyAV	CCTTC	5 cut(s)	35, 242, 352, 837, 1219
HpyCH4III	ACNGT	2 cut(s)	375, 1492
HpyCH4IV	ACGT	3 cut(s)	236, 583, 757
HpyF10VI	GCNNNNNNNGC	6 cut(s)	117, 137, 650, 671, 1240, 1262
HpyF3I	CTNAG	5 cut(s)	277, 303, 762, 990, 1221
HpySE526I	ACGT	3 cut(s)	236, 583, 757
Hsp92II	CATG	3 cut(s)	485, 620, 916
HspAI	GCGC	1 cut(s)	1327
Ksp22I	TGATCA	1 cut(s)	553
Kzo9I	GATC	9 cut(s)	99, 142, 152, 255, 553, 861, 881, 1213, 1358
LguI	GCTCTTC	1 cut(s)	118
LmnI	GCTCC	3 cut(s)	272, 591, 1055
Lsp1109I	GCAGC	6 cut(s)	276, 518, 581, 1027, 1277, 1316
LweI	GCATC	5 cut(s)	98, 328, 520, 1363, 1392
MaeI	CTAG	4 cut(s)	588, 852, 1244, 1413
MaeII	ACGT	3 cut(s)	236, 583, 757
MaeIII	GTNAC	2 cut(s)	10, 875
MalI	GATC	9 cut(s)	101, 144, 154, 257, 555, 863, 883, 1215, 1360
MboI	GATC	9 cut(s)	99, 142, 152, 255, 553, 861, 881, 1213, 1358
MboII	GAAGA	8 cut(s)	40, 43, 46, 105, 214, 1112, 1151, 1259
MfeI	CAATTG	1 cut(s)	724
MflI	RGATCY	3 cut(s)	99, 255, 881
MhlI	GDGCHC	5 cut(s)	92, 165, 236, 285, 583
MluCI	AATT	6 cut(s)	271, 393, 538, 724, 939, 1336
MlyI	GAGTC	2 cut(s)	1055, 1203
MroXI	GAANNNNTTC	1 cut(s)	1116
MseI	TTAA	5 cut(s)	476, 657, 789, 962, 1176
MslI	CAYNNNNRTG	2 cut(s)	890, 1371
MspI	CCGG	3 cut(s)	185, 1167, 1271
MspR9I	CCNGG	3 cut(s)	185, 1167, 1231
MunI	CAATTG	1 cut(s)	724
Mva1269I	GAATGC	1 cut(s)	901
MvaI	CCWGG	1 cut(s)	1231
MwoI	GCNNNNNNNGC	6 cut(s)	117, 137, 650, 671, 1240, 1262
NciI	CCSGG	2 cut(s)	185, 1167
NdeII	GATC	9 cut(s)	99, 142, 152, 255, 553, 861, 881, 1213, 1358
NlaIII	CATG	3 cut(s)	485, 620, 916
NlaIV	GGNNCC	2 cut(s)	257, 883
NmeAIII	GCCGAG	1 cut(s)	970
NmuCI	GTSAC	2 cut(s)	10, 875
NspI	RCATGY	1 cut(s)	916
PceI	AGGCCT	3 cut(s)	82, 471, 1229
PciSI	GCTCTTC	1 cut(s)	118
PcsI	WCGNNNNNNNCGW	2 cut(s)	83, 156
PctI	GAATGC	1 cut(s)	901
PdmI	GAANNNNTTC	1 cut(s)	1116
PfeI	GAWTC	1 cut(s)	1150
PkrI	GCNGC	6 cut(s)	266, 508, 596, 1042, 1267, 1331
PleI	GAGTC	2 cut(s)	1055, 1202
PmaCI	CACGTG	3 cut(s)	237, 584, 758
PmlI	CACGTG	3 cut(s)	237, 584, 758
PpsI	GAGTC	2 cut(s)	1055, 1202
Ppu21I	YACGTR	3 cut(s)	237, 584, 758
PsiI	TTATAA	1 cut(s)	926
Psp124BI	GAGCTC	1 cut(s)	165
Psp6I	CCWGG	1 cut(s)	1229
PspCI	CACGTG	3 cut(s)	237, 584, 758
PspFI	CCCAGC	1 cut(s)	1321
PspGI	CCWGG	1 cut(s)	1229
PspN4I	GGNNCC	2 cut(s)	257, 883
PspPI	GGNCC	6 cut(s)	118, 307, 600, 770, 931, 1409
PstI	CTGCAG	1 cut(s)	1045
PstNI	CAGNNNCTG	1 cut(s)	1240
PsuI	RGATCY	3 cut(s)	99, 255, 881
RsaI	GTAC	4 cut(s)	458, 741, 1032, 1473
RsaNI	GTAC	4 cut(s)	457, 740, 1031, 1472
RseI	CAYNNNNRTG	2 cut(s)	890, 1371
SacI	GAGCTC	1 cut(s)	165
SapI	GCTCTTC	1 cut(s)	118
SaqAI	TTAA	5 cut(s)	476, 657, 789, 962, 1176
SatI	GCNGC	6 cut(s)	265, 507, 595, 1041, 1266, 1330
Sau3AI	GATC	9 cut(s)	99, 142, 152, 255, 553, 861, 881, 1213, 1358
Sau96I	GGNCC	6 cut(s)	118, 307, 600, 770, 931, 1409
SchI	GAGTC	2 cut(s)	1055, 1203
ScrFI	CCNGG	3 cut(s)	185, 1167, 1231
SduI	GDGCHC	5 cut(s)	92, 165, 236, 285, 583
SfaNI	GCATC	5 cut(s)	98, 328, 520, 1363, 1392
SfcI	CTRYAG	2 cut(s)	975, 1041
SinI	GGWCC	3 cut(s)	600, 770, 1409
SmiMI	CAYNNNNRTG	2 cut(s)	890, 1371
SpeI	ACTAGT	1 cut(s)	851
Sse9I	AATT	6 cut(s)	271, 393, 538, 724, 939, 1336
SseBI	AGGCCT	3 cut(s)	82, 471, 1229
SsiI	CCGC	4 cut(s)	73, 168, 319, 859
SspI	AATATT	1 cut(s)	1507
SspMI	CTAG	4 cut(s)	588, 852, 1244, 1413
SstI	GAGCTC	1 cut(s)	165
StuI	AGGCCT	3 cut(s)	82, 471, 1229
StyD4I	CCNGG	3 cut(s)	183, 1165, 1229
StyI	CCWWGG	3 cut(s)	132, 259, 366
TaaI	ACNGT	2 cut(s)	375, 1492
TaiI	ACGT	3 cut(s)	239, 586, 760
TaqI	TCGA	2 cut(s)	345, 450
TasI	AATT	6 cut(s)	271, 393, 538, 724, 939, 1336
TatI	WGTACW	2 cut(s)	456, 1030
TfiI	GAWTC	1 cut(s)	1150
Tru1I	TTAA	5 cut(s)	476, 657, 789, 962, 1176
Tru9I	TTAA	5 cut(s)	476, 657, 789, 962, 1176
TscAI	CASTG	2 cut(s)	285, 570
TseFI	GTSAC	2 cut(s)	10, 875
TseI	GCWGC	6 cut(s)	264, 506, 594, 1040, 1265, 1329
Tsp45I	GTSAC	2 cut(s)	10, 875
TspDTI	ATGAA	6 cut(s)	633, 908, 1100, 1155, 1275, 1355
TspGWI	ACGGA	1 cut(s)	894
TspRI	CASTG	2 cut(s)	285, 570
VpaK11BI	GGWCC	3 cut(s)	600, 770, 1409
XagI	CCTNNNNNAGG	1 cut(s)	125
XapI	RAATTY	2 cut(s)	538, 1336
XceI	RCATGY	1 cut(s)	916
XmnI	GAANNNNTTC	1 cut(s)	1116
XspI	CTAG	4 cut(s)	588, 852, 1244, 1413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.