Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003821

Physical Location & Seq

Genomic Coordinates Forward (+)

21019760 .. 21022319

Gene Structure 2560 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1134200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1731 bp

ATGCAGGTTAATTTTGTGGATTCTTCTCAGTTTGTCTATGGAAACAGGCAGGTTCTGAAACAGAGAGACTTTCGGAAATTGGCCTCAGATTCGAGTTCTTGCAGTAGTGGCTCTACCGGCGAAGACTGGTTCCCCTCTGAATTGGGCAGGAGATCTTCACAGCAAGCTGTTGGAACTCCAATAAAGAAGTTATTGGCTGAGGAGATGGTGAGAGAAACCGAACCTAGAAGGAGATCACCAAGCTTAATTGCCAAATTGATGGGTCTTGATGGGCTGCCACCTCAGCAGCCTGCTGATAGACAATCAAAGGGCATTTCAGAGAATTATCTTCAGAAGAAAAGATCGGTAGAGAAAGAGCACAGAAGCAGTATGTCTCATGACCGCCATTCATCTAGGAAGAACTCAAGGGTGCAGCAGGAGTTTAAGGATGTGTTTGAAGTTTATGAACCTGCAAGGATTTACTCATCTCGAGGAAGTGCTAACCCAAAGCTCAGTGATGCTGAAATGGCTTTTGTTCGACAGAAATTCATGGATGCAAAACGCCTTTCAACAGATGAGAGGCTACAGGATTCAAAGGAGTTCCATGATGTGCTTGAGGTGCTGGAATCTAACAAGGATATTCTATTGAAATTCCTTCAGCAACCAGACTCATTGTTCACAAAACATATGCATGATCTGCAAGGTGGTCCTCAATCCCTTTGTGGTCGCATAGCATCTTTGAAGCCATCAGAAGCTCAAAAGTATGAAAACATTGACCTTGGCTTCACATCAGCAAGAGAGCAGAAGCATCGCTGTAAATCTCCTCAGAAGCATCGGGATTCTTTTTCTAGCCACTCTGACAGTAGACATCCTCTTGAATCATCACTAAACCGACCAGAAGTGAAAATTGAATCTGCCATTCATCCTACAATTCATCCTACAAGGATAGTTGTTCTGAAACCAAACCTTGGGAAGGTGCTGAATGCTCCCAAAACATCACCTTGTTCTCCTCGTGCTTCTATGTTAGATGGTGGCAAACACGCTGAATTTCTGAGCATTAGAAACAGGGAGGCAGAATCATGTGGAAGGAAAAACGTCCAAGATAATGATGGGCATCTGAGACACAAGTCTAGAGAATCTAGAGAAATTGCTAAGGAAATCACAAGGAAAATGAGAAATAACTTTAGCAGTAATTCTGTGCGTCTTTCCTCCTCTGGTTTGAAAGGATATGCTGGTGATGAGAGTTCTTGTAGCATGTCTGAGAATGAATCTGCAAACGAGTCAGAGAGGATGTCGGTGGCTTCCAGGCATTCCTTTCACATAAGTAACCACTCCAGGCCTTCATCGTCGTGCTCTACTGAATCATCTGTGAGTAGAGAGGCCAAGAAGAGGCTCTCAGAGAGGTGGAAACTGACACACAAGTCCCAAGAAATGGGACCTGTGAGCAGGGGCAGCACACTTGGTGAAATGCTTGCTGTCCCAGACAAGGAAACGCGAGCTGCTAATTTGAATGCCATGATTGGTGAGGCAGGATTCAGAGATACATTTTCTACTGAAGGGGTGGACCTCTGGGTATCAGCAGTAGGGATGGTTGGAAGGATGGATGCATCAATAGTTTATCAAGATCAAAATCTCTTCCTTCTTCATCTAGTGTGTTTGGAAGTTTTAAAACAAGTATGCGGTGTGAAACTGTACATAATGACAAGTATCCGATGCCAAAGGATACAGTCCCGCACGAAAGACACCGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3741 DUF4378 VARLMGL

Protein Analysis

577

Amino Acids

64.88

Weight (kDa)

9.44

Isoelectric Point (pI)

62.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VARLMGL	PF14383	73 - 96	6.8e-07	DUF761-associated sequence motif
DUF3741	PF12552	173 - 217	2.1e-21	Protein of unknown function (DUF3741)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003821)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53540 AT3G53540
fragaria_vesca	FvH4_6g14620 FvH4_6g14620 FvH4_6g14620 FvH4_6g14620 FvH4_6g14620 FvH4_6g14620 FvH4_6g14620 FvH4_6g14620
malus_domestica	MD04G1118500.v1.1 MD12G1134100.v1.1 MD12G1134200.v1.1 MD12G1134300.v1.1 MD12G1134400.v1.1
prunus_persica	Prupe.6G247900_v2.0.a1 Prupe.6G247900_v2.0.a1
pyrus_communis	pycom04g10820 pycom12g12970
rosa_chinensis	RchiOBHm_Chr3g0467721
rosa_laevigata	RLG00000024478
rosa_multiflora	Rmu_co8048390.1_g000001 Rmu_co8092630.1_g000001 Rmu_co8309069.1_g000001 Rmu_sc0001793.1_g000010 Rmu_sc0013459.1_g000002
rosa_roxburghii	Rroxscaffold_6G00413480
rosa_rugosa	Rorug03G0089200 Rorug03G0089200
rosa_samantha	Rh3AG137700 Rh3BG159000 Rh3CG158900 Rh3DG159500
rosa_wichuraiana	Rw3G013040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1399
Acc36I	ACCTGC	2 cut(s)	40, 457
AccB7I	CCANNNNNTGG	2 cut(s)	947, 1411
AccI	GTMKAC	1 cut(s)	844
AccII	CGCG	1 cut(s)	1474
AciI	CCGC	3 cut(s)	382, 1659, 1711
AcsI	RAATTY	3 cut(s)	524, 629, 1025
AcuI	CTGAAG	3 cut(s)	314, 620, 1554
AdeI	CACNNNGTG	1 cut(s)	1442
AfaI	GTAC	1 cut(s)	1673
AfiI	CCNNNNNNNGG	5 cut(s)	947, 952, 1368, 1411, 1465
AgsI	TTSAA	9 cut(s)	437, 549, 573, 628, 721, 857, 890, 1201, 1489
AjnI	CCWGG	2 cut(s)	1283, 1313
AluBI	AGCT	5 cut(s)	167, 243, 490, 734, 1478
AluI	AGCT	5 cut(s)	167, 243, 490, 734, 1478
Alw21I	GWGCWC	2 cut(s)	360, 1334
Alw26I	GTCTC	3 cut(s)	60, 378, 1093
AlwNI	CAGNNNCTG	1 cut(s)	55
Ama87I	CYCGRG	1 cut(s)	468
AoxI	GGCC	3 cut(s)	81, 1316, 1359
ApeKI	GCWGC	5 cut(s)	274, 286, 412, 1431, 1478
ApoI	RAATTY	3 cut(s)	524, 629, 1025
AspS9I	GGNCC	3 cut(s)	686, 1415, 1543
AsuHPI	GGTGA	6 cut(s)	220, 228, 969, 1226, 1454, 1514
AvaI	CYCGRG	1 cut(s)	468
AvaII	GGWCC	3 cut(s)	686, 1415, 1543
BauI	CACGAG	1 cut(s)	990
BbsI	GAAGAC	1 cut(s)	129
Bbv12I	GWGCWC	2 cut(s)	360, 1334
BbvCI	CCTCAGC	2 cut(s)	198, 282
BbvI	GCAGC	5 cut(s)	261, 298, 424, 1443, 1465
BccI	CCATC	8 cut(s)	199, 253, 263, 733, 1001, 1082, 1561, 1573
BcgI	CGANNNNNNTGC	2 cut(s)	770, 804
BciT130I	CCWGG	2 cut(s)	1285, 1315
BciVI	GTATCC	2 cut(s)	1695, 1697
BcoDI	GTCTC	3 cut(s)	60, 378, 1093
BfaI	CTAG	6 cut(s)	225, 393, 828, 1110, 1119, 1628
BfmI	CTRYAG	1 cut(s)	563
BfuAI	ACCTGC	2 cut(s)	40, 457
BfuI	GTATCC	2 cut(s)	1695, 1697
BglII	AGATCT	1 cut(s)	152
BisI	GCNGC	5 cut(s)	275, 287, 413, 1432, 1479
BlsI	GCNGC	5 cut(s)	276, 288, 414, 1433, 1480
Bme1390I	CCNGG	2 cut(s)	1285, 1315
Bme18I	GGWCC	3 cut(s)	686, 1415, 1543
BmeT110I	CYCGRG	1 cut(s)	468
BmgT120I	GGNCC	3 cut(s)	686, 1415, 1543
BmiI	GGNNCC	2 cut(s)	131, 1416
BmrFI	CCNGG	2 cut(s)	1285, 1315
BmsI	GCATC	9 cut(s)	487, 523, 722, 796, 820, 1102, 1573, 1595, 1682
BoxI	GACNNNNGTC	1 cut(s)	1105
BpiI	GAAGAC	1 cut(s)	129
BpmI	CTGGAG	1 cut(s)	1297
Bpu10I	CCTNAGC	3 cut(s)	198, 282, 1131
BpuEI	CTTGAG	2 cut(s)	388, 614
BsaBI	GATNNNNATC	2 cut(s)	1092, 1608
BsaJI	CCNNGG	2 cut(s)	757, 946
Bsc4I	CCNNNNNNNGG	5 cut(s)	947, 952, 1368, 1411, 1465
Bse118I	RCCGGY	1 cut(s)	116
Bse1I	ACTGG	1 cut(s)	131
Bse8I	GATNNNNATC	2 cut(s)	1092, 1608
BseDI	CCNNGG	2 cut(s)	757, 946
BseGI	GGATG	9 cut(s)	433, 538, 847, 901, 913, 1275, 1572, 1584, 1588
BseJI	GATNNNNATC	2 cut(s)	1092, 1608
BseLI	CCNNNNNNNGG	5 cut(s)	947, 952, 1368, 1411, 1465
BseNI	ACTGG	1 cut(s)	131
BseRI	GAGGAG	4 cut(s)	215, 792, 978, 1180
BseXI	GCAGC	5 cut(s)	261, 298, 424, 1443, 1465
BsgI	GTGCAG	1 cut(s)	431
Bsh1236I	CGCG	1 cut(s)	1474
BshFI	GGCC	3 cut(s)	83, 1318, 1361
BsiHKAI	GWGCWC	2 cut(s)	360, 1334
BsiHKCI	CYCGRG	1 cut(s)	468
BsiSI	CCGG	1 cut(s)	117
BslFI	GGGAC	4 cut(s)	1387, 1428, 1442, 1693
BslI	CCNNNNNNNGG	5 cut(s)	947, 952, 1368, 1411, 1465
BsmAI	GTCTC	3 cut(s)	60, 378, 1093
BsmFI	GGGAC	4 cut(s)	1387, 1428, 1442, 1693
BsmI	GAATGC	3 cut(s)	967, 1288, 1495
BsnI	GGCC	3 cut(s)	83, 1318, 1361
BsoBI	CYCGRG	1 cut(s)	468
Bsp1286I	GDGCHC	2 cut(s)	360, 1334
Bsp1407I	TGTACA	1 cut(s)	1671
Bsp143I	GATC	5 cut(s)	152, 233, 341, 673, 1603
BspACI	CCGC	3 cut(s)	382, 1659, 1711
BspANI	GGCC	3 cut(s)	83, 1318, 1361
BspFNI	CGCG	1 cut(s)	1474
BspHI	TCATGA	1 cut(s)	376
BspLI	GGNNCC	2 cut(s)	131, 1416
BspMI	ACCTGC	2 cut(s)	40, 457
BsrFI	RCCGGY	1 cut(s)	116
BsrGI	TGTACA	1 cut(s)	1671
BsrI	ACTGG	1 cut(s)	131
BssAI	RCCGGY	1 cut(s)	116
BssECI	CCNNGG	2 cut(s)	757, 946
BssMI	GATC	5 cut(s)	152, 233, 341, 673, 1603
BssSI	CACGAG	1 cut(s)	990
BssT1I	CCWWGG	2 cut(s)	757, 946
Bst2BI	CACGAG	1 cut(s)	990
Bst2UI	CCWGG	2 cut(s)	1285, 1315
Bst4CI	ACNGT	4 cut(s)	842, 1671, 1707, 1726
Bst6I	CTCTTC	2 cut(s)	1361, 1619
BstAPI	GCANNNNNTGC	1 cut(s)	676
BstAUI	TGTACA	1 cut(s)	1671
BstC8I	GCNNGC	4 cut(s)	165, 291, 1452, 1476
BstENI	CCTNNNNNAGG	1 cut(s)	950
BstF5I	GGATG	9 cut(s)	433, 538, 847, 901, 913, 1275, 1572, 1584, 1588
BstFNI	CGCG	1 cut(s)	1474
BstKTI	GATC	5 cut(s)	155, 236, 344, 676, 1606
BstMAI	GTCTC	3 cut(s)	60, 378, 1093
BstMBI	GATC	5 cut(s)	152, 233, 341, 673, 1603
BstMWI	GCNNNNNNNGC	7 cut(s)	108, 117, 283, 506, 598, 676, 1431
BstNI	CCWGG	2 cut(s)	1285, 1315
BstNSI	RCATGY	1 cut(s)	1237
BstPAI	GACNNNNGTC	1 cut(s)	1105
BstSCI	CCNGG	2 cut(s)	1283, 1313
BstSFI	CTRYAG	1 cut(s)	563
BstUI	CGCG	1 cut(s)	1474
BstV1I	GCAGC	5 cut(s)	261, 298, 424, 1443, 1465
BstV2I	GAAGAC	1 cut(s)	129
BstX2I	RGATCY	1 cut(s)	152
BstXI	CCANNNNNNTGG	1 cut(s)	259
BstYI	RGATCY	1 cut(s)	152
BsuI	GTATCC	2 cut(s)	1695, 1697
BsuRI	GGCC	3 cut(s)	83, 1318, 1361
BtgZI	GCGATG	1 cut(s)	773
BtsCI	GGATG	9 cut(s)	433, 538, 847, 901, 913, 1275, 1572, 1584, 1588
BtsIMutI	CAGTG	1 cut(s)	499
BveI	ACCTGC	2 cut(s)	40, 457
Cac8I	GCNNGC	4 cut(s)	165, 291, 1452, 1476
CaiI	CAGNNNCTG	1 cut(s)	55
CciI	TCATGA	1 cut(s)	376
Cfr10I	RCCGGY	1 cut(s)	116
Cfr13I	GGNCC	3 cut(s)	686, 1415, 1543
CseI	GACGC	1 cut(s)	1169
Csp6I	GTAC	1 cut(s)	1672
CviAII	CATG	7 cut(s)	377, 529, 584, 671, 1059, 1234, 1495
CviQI	GTAC	1 cut(s)	1672
DpnI	GATC	5 cut(s)	154, 235, 343, 675, 1605
DpnII	GATC	5 cut(s)	152, 233, 341, 673, 1603
DraI	TTTAAA	1 cut(s)	1647
DraIII	CACNNNGTG	1 cut(s)	1442
DrdI	GACNNNNNNGTC	1 cut(s)	1399
DseDI	GACNNNNNNGTC	1 cut(s)	1399
Eam1104I	CTCTTC	2 cut(s)	1361, 1619
EarI	CTCTTC	2 cut(s)	1361, 1619
Eco130I	CCWWGG	2 cut(s)	757, 946
Eco147I	AGGCCT	1 cut(s)	1318
Eco47I	GGWCC	3 cut(s)	686, 1415, 1543
Eco57I	CTGAAG	3 cut(s)	314, 620, 1554
Eco88I	CYCGRG	1 cut(s)	468
EcoNI	CCTNNNNNAGG	1 cut(s)	950
EcoO109I	RGGNCCY	1 cut(s)	1415
EcoRII	CCWGG	2 cut(s)	1283, 1313
EcoT14I	CCWWGG	2 cut(s)	757, 946
EcoT22I	ATGCAT	2 cut(s)	672, 1588
ErhI	CCWWGG	2 cut(s)	757, 946
FaeI	CATG	7 cut(s)	380, 532, 587, 674, 1062, 1237, 1498
FaqI	GGGAC	4 cut(s)	1387, 1428, 1442, 1693
FatI	CATG	7 cut(s)	376, 528, 583, 670, 1058, 1233, 1494
FauI	CCCGC	1 cut(s)	1718
FauNDI	CATATG	1 cut(s)	666
FblI	GTMKAC	1 cut(s)	844
Fnu4HI	GCNGC	5 cut(s)	275, 287, 413, 1432, 1479
FokI	GGATG	9 cut(s)	440, 545, 834, 888, 900, 1282, 1579, 1591, 1595
Fsp4HI	GCNGC	5 cut(s)	275, 287, 413, 1432, 1479
FspBI	CTAG	6 cut(s)	225, 393, 828, 1110, 1119, 1628
GluI	GCNGC	5 cut(s)	275, 287, 413, 1432, 1479
GsuI	CTGGAG	1 cut(s)	1297
HaeIII	GGCC	3 cut(s)	83, 1318, 1361
HapII	CCGG	1 cut(s)	117
HgaI	GACGC	1 cut(s)	1169
Hin1II	CATG	7 cut(s)	380, 532, 587, 674, 1062, 1237, 1498
HindIII	AAGCTT	1 cut(s)	241
HpaII	CCGG	1 cut(s)	117
HphI	GGTGA	6 cut(s)	220, 228, 969, 1226, 1454, 1514
Hpy166II	GTNNAC	3 cut(s)	657, 845, 1543
Hpy188III	TCNNGA	8 cut(s)	266, 377, 468, 815, 854, 1110, 1119, 1601
Hpy8I	GTNNAC	3 cut(s)	657, 845, 1543
Hpy99I	CGWCG	1 cut(s)	1330
HpyAV	CCTTC	8 cut(s)	222, 644, 946, 1059, 1329, 1529, 1569, 1628
HpyCH4III	ACNGT	4 cut(s)	842, 1671, 1707, 1726
HpyCH4IV	ACGT	1 cut(s)	1074
HpyCH4V	TGCA	9 cut(s)	4, 102, 412, 452, 536, 670, 679, 1253, 1586
HpyF10VI	GCNNNNNNNGC	7 cut(s)	108, 117, 283, 506, 598, 676, 1431
HpySE526I	ACGT	1 cut(s)	1074
Hsp92II	CATG	7 cut(s)	380, 532, 587, 674, 1062, 1237, 1498
Kzo9I	GATC	5 cut(s)	152, 233, 341, 673, 1603
LmnI	GCTCC	1 cut(s)	970
Lsp1109I	GCAGC	5 cut(s)	261, 298, 424, 1443, 1465
LweI	GCATC	9 cut(s)	487, 523, 722, 796, 820, 1102, 1573, 1595, 1682
MaeI	CTAG	6 cut(s)	225, 393, 828, 1110, 1119, 1628
MaeII	ACGT	1 cut(s)	1074
MaeIII	GTNAC	1 cut(s)	1304
MalI	GATC	5 cut(s)	154, 235, 343, 675, 1605
MboI	GATC	5 cut(s)	152, 233, 341, 673, 1603
MboII	GAAGA	9 cut(s)	15, 134, 147, 320, 346, 409, 1378, 1606, 1613
MflI	RGATCY	1 cut(s)	152
MhlI	GDGCHC	2 cut(s)	360, 1334
MlyI	GAGTC	2 cut(s)	641, 1268
MmeI	TCCRAC	2 cut(s)	151, 1552
Mph1103I	ATGCAT	2 cut(s)	672, 1588
MseI	TTAA	4 cut(s)	9, 245, 423, 1646
MslI	CAYNNNNRTG	2 cut(s)	669, 1327
MspI	CCGG	1 cut(s)	117
MspR9I	CCNGG	2 cut(s)	1285, 1315
Mva1269I	GAATGC	3 cut(s)	967, 1288, 1495
MvaI	CCWGG	2 cut(s)	1285, 1315
MvnI	CGCG	1 cut(s)	1474
MwoI	GCNNNNNNNGC	7 cut(s)	108, 117, 283, 506, 598, 676, 1431
NdeI	CATATG	1 cut(s)	666
NdeII	GATC	5 cut(s)	152, 233, 341, 673, 1603
NlaIII	CATG	7 cut(s)	380, 532, 587, 674, 1062, 1237, 1498
NlaIV	GGNNCC	2 cut(s)	131, 1416
NsiI	ATGCAT	2 cut(s)	672, 1588
NspI	RCATGY	1 cut(s)	1237
PaeR7I	CTCGAG	1 cut(s)	468
PagI	TCATGA	1 cut(s)	376
PceI	AGGCCT	1 cut(s)	1318
PctI	GAATGC	3 cut(s)	967, 1288, 1495
PflMI	CCANNNNNTGG	2 cut(s)	947, 1411
PkrI	GCNGC	5 cut(s)	276, 288, 414, 1433, 1480
PleI	GAGTC	2 cut(s)	641, 1267
PpsI	GAGTC	2 cut(s)	641, 1267
PpuMI	RGGWCCY	1 cut(s)	1415
PshAI	GACNNNNGTC	1 cut(s)	1105
Psp5II	RGGWCCY	1 cut(s)	1415
Psp6I	CCWGG	2 cut(s)	1283, 1313
PspGI	CCWGG	2 cut(s)	1283, 1313
PspN4I	GGNNCC	2 cut(s)	131, 1416
PspPI	GGNCC	3 cut(s)	686, 1415, 1543
PspPPI	RGGWCCY	1 cut(s)	1415
PstNI	CAGNNNCTG	1 cut(s)	55
PsuI	RGATCY	1 cut(s)	152
RsaI	GTAC	1 cut(s)	1673
RsaNI	GTAC	1 cut(s)	1672
RseI	CAYNNNNRTG	2 cut(s)	669, 1327
SaqAI	TTAA	4 cut(s)	9, 245, 423, 1646
SatI	GCNGC	5 cut(s)	275, 287, 413, 1432, 1479
Sau3AI	GATC	5 cut(s)	152, 233, 341, 673, 1603
Sau96I	GGNCC	3 cut(s)	686, 1415, 1543
SchI	GAGTC	2 cut(s)	641, 1268
ScrFI	CCNGG	2 cut(s)	1285, 1315
SduI	GDGCHC	2 cut(s)	360, 1334
SfaNI	GCATC	9 cut(s)	487, 523, 722, 796, 820, 1102, 1573, 1595, 1682
SfcI	CTRYAG	1 cut(s)	563
Sfr274I	CTCGAG	1 cut(s)	468
SinI	GGWCC	3 cut(s)	686, 1415, 1543
SlaI	CTCGAG	1 cut(s)	468
SmiMI	CAYNNNNRTG	2 cut(s)	669, 1327
SmlI	CTYRAG	3 cut(s)	403, 468, 593
SmoI	CTYRAG	3 cut(s)	403, 468, 593
SseBI	AGGCCT	1 cut(s)	1318
SsiI	CCGC	3 cut(s)	382, 1659, 1711
SspMI	CTAG	6 cut(s)	225, 393, 828, 1110, 1119, 1628
StuI	AGGCCT	1 cut(s)	1318
StyD4I	CCNGG	2 cut(s)	1283, 1313
StyI	CCWWGG	2 cut(s)	757, 946
TaaI	ACNGT	4 cut(s)	842, 1671, 1707, 1726
TaiI	ACGT	1 cut(s)	1077
TaqI	TCGA	3 cut(s)	92, 469, 517
TatI	WGTACW	1 cut(s)	1671
Tru1I	TTAA	4 cut(s)	9, 245, 423, 1646
Tru9I	TTAA	4 cut(s)	9, 245, 423, 1646
TscAI	CASTG	1 cut(s)	499
TseI	GCWGC	5 cut(s)	274, 286, 412, 1431, 1478
TspDTI	ATGAA	9 cut(s)	378, 459, 517, 759, 890, 902, 1260, 1311, 1613
TspRI	CASTG	1 cut(s)	499
Van91I	CCANNNNNTGG	2 cut(s)	947, 1411
VpaK11BI	GGWCC	3 cut(s)	686, 1415, 1543
XagI	CCTNNNNNAGG	1 cut(s)	950
XapI	RAATTY	3 cut(s)	524, 629, 1025
XbaI	TCTAGA	2 cut(s)	1109, 1118
XceI	RCATGY	1 cut(s)	1237
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1085
XhoI	CTCGAG	1 cut(s)	468
XmiI	GTMKAC	1 cut(s)	844
XspI	CTAG	6 cut(s)	225, 393, 828, 1110, 1119, 1628
Zsp2I	ATGCAT	2 cut(s)	672, 1588

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.