MD12G1134300.v1.1

Protein of unknown function (DUF3741)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003821
Physical Location & Seq
Forward (+)
21022321 .. 21024475
2155 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1134300.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGATGCAAGTCCAGTTTCGGCAAATTTGGTGGATGTGGATGCATCCAAGCCTTCTGAAACTCCTGACGTGTTCCTTCCAGAATTATCATCTCATATGTCGGTAGAAGGCTATTCATCTGGTGGACACCAAGATAATGCAATTCCACAGGAACCCTCAATCAGGCCACCCCATGAACAGGCAGTCCCCTCTAACCAGTCTGTACCTGGAATCGAATCTCCTGCAAGCTCCAAGGAAGCTGAGCAGCCCAGTCCGGTTTCAGTTCTGGAAGTTCCTTTTACTGATGATGTGTCATCTAGTTCTGAATGCTTTGAGAGTGTCAGTGCTGACCTGCAAGGGCTTCGGATGCAGCTTAAGCTACTGAAGTTGGAGTCAGAACCATATGAAGAGGGACACATGGAAGTGTCGAGTGATGATGAAGTTGGTGATGGATCTACTGGGTTGTCAGATGCAAGAGGATTTTGTAAAGATGAAAAGAGCTGGGAGTCTGTATACTTGGCCGATACCTTAACTGAATCTGGTTTAAACAATGCTGATCCGGCTACCTTCTTGGCAATGTGGCACACCCCAGAATGTCCTGTGAATCCTTTACTATTCGAAGAACTGGAGAAGAAGTACTACGGTCAGACTTCTTGGCCAAAGCCCGAGAGGAAATTACTGTTCGATCGAATCAATTCAGGGCTCGTGGAGATGTTTGAGCAATTCACTGATCCATACCCATGGGTAAGGCCTGCGAGCAAAAGAATTGGTCCCAAGTGGATTAATCCAAGTGCAGCCGAAGATGTTCTGTGCACATTGCTAGCAAGCCAAGAAGAGAACGCGAACGAGGACAGTCTGGAGAAAGAGCTGCAACGTGACTTGCTGTGGCTGGATTTTGGAGACGAAATAGACGTAATAGGTAGGGAAGTAGAGAGGAAGTTGATAGAAGAGCTAGTAGCAGAGGTAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378 VARLMGL

Protein Analysis

317

Amino Acids

34.99

Weight (kDa)

4.25

Isoelectric Point (pI)

59.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 163 - 314 3.4e-35 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 339
AccI GTMKAC 1 cut(s) 492
AccII CGCG 1 cut(s) 821
AclWI GGATC 3 cut(s) 439, 530, 704
AcoI YGGCCR 2 cut(s) 498, 635
AcsI RAATTY 1 cut(s) 25
AcuI CTGAAG 1 cut(s) 383
AfaI GTAC 2 cut(s) 204, 617
AfiI CCNNNNNNNGG 2 cut(s) 162, 178
AflII CTTAAG 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 69
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 205
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
AluBI AGCT 7 cut(s) 228, 239, 352, 358, 480, 847, 931
AluI AGCT 7 cut(s) 228, 239, 352, 358, 480, 847, 931
Alw21I GWGCWC 1 cut(s) 794
Alw26I GTCTC 1 cut(s) 873
Alw44I GTGCAC 1 cut(s) 790
AlwI GGATC 3 cut(s) 439, 530, 704
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 4 cut(s) 164, 498, 635, 728
ApaLI GTGCAC 1 cut(s) 790
ApeKI GCWGC 4 cut(s) 244, 349, 773, 847
ApoI RAATTY 1 cut(s) 25
AseI ATTAAT 1 cut(s) 762
Asp700I GAANNNNTTC 2 cut(s) 673, 783
AspS9I GGNCC 1 cut(s) 749
AsuHPI GGTGA 1 cut(s) 437
AsuII TTCGAA 1 cut(s) 597
AsuNHI GCTAGC 1 cut(s) 799
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 1 cut(s) 749
BaeGI GKGCMC 1 cut(s) 794
BalI TGGCCA 1 cut(s) 637
BanII GRGCYC 1 cut(s) 684
BarI GAAGNNNNNNTAC 2 cut(s) 602, 634
BauI CACGAG 1 cut(s) 683
Bbv12I GWGCWC 1 cut(s) 794
BbvI GCAGC 4 cut(s) 256, 361, 785, 834
BccI CCATC 1 cut(s) 422
BcgI CGANNNNNNTGC 4 cut(s) 203, 237, 323, 357
BciT130I CCWGG 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 873
BfaI CTAG 3 cut(s) 297, 800, 932
BfrI CTTAAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 339
BisI GCNGC 4 cut(s) 245, 350, 774, 848
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 4 cut(s) 246, 351, 775, 849
BmcAI AGTACT 1 cut(s) 617
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 749
BmeT110I CYCGRG 1 cut(s) 644
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 2 cut(s) 153, 751
BmrFI CCNGG 1 cut(s) 207
BmrI ACTGGG 2 cut(s) 243, 447
BmsI GCATC 4 cut(s) 31, 53, 336, 439
BmtI GCTAGC 1 cut(s) 803
BmuI ACTGGG 2 cut(s) 243, 447
BpmI CTGGAG 2 cut(s) 626, 857
Bpu1102I GCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 597
BsaJI CCNNGG 2 cut(s) 231, 719
BsaWI WCCGGW 1 cut(s) 253
BsaXI ACNNNNNCTCC 2 cut(s) 599, 629
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 178
Bse1I ACTGG 5 cut(s) 14, 196, 249, 442, 609
Bse3DI GCAATG 2 cut(s) 561, 794
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 231, 719
BseGI GGATG 5 cut(s) 10, 40, 44, 46, 351
BseLI CCNNNNNNNGG 2 cut(s) 162, 178
BseMI GCAATG 2 cut(s) 561, 794
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 5 cut(s) 14, 196, 249, 442, 609
BseSI GKGCMC 1 cut(s) 794
BseXI GCAGC 4 cut(s) 256, 361, 785, 834
BseYI CCCAGC 1 cut(s) 480
BsgI GTGCAG 1 cut(s) 792
Bsh1236I CGCG 1 cut(s) 821
Bsh1285I CGRYCG 1 cut(s) 667
BshFI GGCC 4 cut(s) 166, 500, 637, 730
BsiEI CGRYCG 1 cut(s) 667
BsiHKAI GWGCWC 1 cut(s) 794
BsiHKCI CYCGRG 1 cut(s) 644
BsiSI CCGG 2 cut(s) 254, 539
BslFI GGGAC 3 cut(s) 170, 405, 735
BslI CCNNNNNNNGG 2 cut(s) 162, 178
BsmAI GTCTC 1 cut(s) 873
BsmBI CGTCTC 1 cut(s) 873
BsmFI GGGAC 3 cut(s) 170, 405, 735
BsmI GAATGC 1 cut(s) 311
BsnI GGCC 4 cut(s) 166, 500, 637, 730
BsoBI CYCGRG 1 cut(s) 644
Bsp119I TTCGAA 1 cut(s) 597
Bsp1286I GDGCHC 2 cut(s) 684, 794
Bsp143I GATC 4 cut(s) 431, 535, 664, 709
Bsp1720I GCTNAGC 1 cut(s) 240
Bsp19I CCATGG 1 cut(s) 719
BspANI GGCC 4 cut(s) 166, 500, 637, 730
BspCNI CTCAG 1 cut(s) 232
BspFNI CGCG 1 cut(s) 821
BspLI GGNNCC 2 cut(s) 153, 751
BspMI ACCTGC 1 cut(s) 339
BspOI GCTAGC 1 cut(s) 803
BspPI GGATC 3 cut(s) 439, 530, 704
BspQI GCTCTTC 1 cut(s) 921
BspT104I TTCGAA 1 cut(s) 597
BspTI CTTAAG 1 cut(s) 353
BsrDI GCAATG 2 cut(s) 561, 794
BsrI ACTGG 5 cut(s) 14, 196, 249, 442, 609
BssECI CCNNGG 2 cut(s) 231, 719
BssMI GATC 4 cut(s) 431, 535, 664, 709
BssNAI GTATAC 1 cut(s) 493
BssSI CACGAG 1 cut(s) 683
BssT1I CCWWGG 2 cut(s) 231, 719
Bst1107I GTATAC 1 cut(s) 493
Bst2BI CACGAG 1 cut(s) 683
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 3 cut(s) 623, 660, 833
Bst6I CTCTTC 3 cut(s) 381, 807, 921
BstAFI CTTAAG 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 597
BstC8I GCNNGC 5 cut(s) 226, 732, 736, 801, 805
BstDEI CTNAG 1 cut(s) 240
BstDSI CCRYGG 1 cut(s) 719
BstENI CCTNNNNNAGG 1 cut(s) 160
BstF5I GGATG 5 cut(s) 10, 40, 44, 46, 351
BstFNI CGCG 1 cut(s) 821
BstKTI GATC 4 cut(s) 434, 538, 667, 712
BstMAI GTCTC 1 cut(s) 873
BstMBI GATC 4 cut(s) 431, 535, 664, 709
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 3 cut(s) 346, 355, 539
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 205
BstSLI GKGCMC 1 cut(s) 794
BstUI CGCG 1 cut(s) 821
BstV1I GCAGC 4 cut(s) 256, 361, 785, 834
BstX2I RGATCY 1 cut(s) 431
BstXI CCANNNNNNTGG 1 cut(s) 720
BstYI RGATCY 1 cut(s) 431
BstZ17I GTATAC 1 cut(s) 493
BsuRI GGCC 4 cut(s) 166, 500, 637, 730
BtgI CCRYGG 1 cut(s) 719
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 5 cut(s) 10, 40, 44, 46, 351
BtsIMutI CAGTG 2 cut(s) 328, 705
BveI ACCTGC 1 cut(s) 339
Cac8I GCNNGC 5 cut(s) 226, 732, 736, 801, 805
Cfr13I GGNCC 1 cut(s) 749
Csp6I GTAC 2 cut(s) 203, 616
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 3 cut(s) 173, 397, 720
CviQI GTAC 2 cut(s) 203, 616
DdeI CTNAG 1 cut(s) 240
DpnI GATC 4 cut(s) 433, 537, 666, 711
DpnII GATC 4 cut(s) 431, 535, 664, 709
DraI TTTAAA 1 cut(s) 525
EaeI YGGCCR 2 cut(s) 498, 635
Eam1104I CTCTTC 3 cut(s) 381, 807, 921
EarI CTCTTC 3 cut(s) 381, 807, 921
Eco130I CCWWGG 2 cut(s) 231, 719
Eco147I AGGCCT 1 cut(s) 730
Eco24I GRGCYC 1 cut(s) 684
Eco47I GGWCC 1 cut(s) 749
Eco57I CTGAAG 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 644
EcoNI CCTNNNNNAGG 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 231, 719
EcoT22I ATGCAT 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 684
ErhI CCWWGG 2 cut(s) 231, 719
Esp3I CGTCTC 1 cut(s) 873
FaeI CATG 3 cut(s) 176, 400, 723
FaiI YATR 9 cut(s) 96, 98, 174, 382, 384, 398, 493, 715, 721
FaqI GGGAC 3 cut(s) 170, 405, 735
FatI CATG 3 cut(s) 172, 396, 719
FauNDI CATATG 2 cut(s) 96, 382
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 4 cut(s) 245, 350, 774, 848
FokI GGATG 5 cut(s) 17, 31, 47, 53, 358
FriOI GRGCYC 1 cut(s) 684
Fsp4HI GCNGC 4 cut(s) 245, 350, 774, 848
FspBI CTAG 3 cut(s) 297, 800, 932
GluI GCNGC 4 cut(s) 245, 350, 774, 848
GsaI CCCAGC 1 cut(s) 484
GsuI CTGGAG 2 cut(s) 626, 857
HaeIII GGCC 4 cut(s) 166, 500, 637, 730
HapII CCGG 2 cut(s) 254, 539
Hin1II CATG 3 cut(s) 176, 400, 723
HinfI GANTC 7 cut(s) 210, 215, 371, 485, 515, 583, 669
HpaII CCGG 2 cut(s) 254, 539
HphI GGTGA 1 cut(s) 437
Hpy166II GTNNAC 3 cut(s) 125, 493, 792
Hpy188I TCNGA 6 cut(s) 58, 304, 345, 376, 448, 627
Hpy188III TCNNGA 4 cut(s) 65, 80, 266, 836
Hpy8I GTNNAC 3 cut(s) 125, 493, 792
HpyAV CCTTC 4 cut(s) 63, 86, 101, 556
HpyCH4III ACNGT 3 cut(s) 623, 660, 833
HpyCH4IV ACGT 3 cut(s) 69, 853, 891
HpyF10VI GCNNNNNNNGC 3 cut(s) 346, 355, 539
HpyF3I CTNAG 1 cut(s) 240
HpySE526I ACGT 3 cut(s) 69, 853, 891
Hsp92II CATG 3 cut(s) 176, 400, 723
Kzo9I GATC 4 cut(s) 431, 535, 664, 709
LguI GCTCTTC 1 cut(s) 921
LmnI GCTCC 1 cut(s) 233
Lsp1109I GCAGC 4 cut(s) 256, 361, 785, 834
LweI GCATC 4 cut(s) 31, 53, 336, 439
MaeI CTAG 3 cut(s) 297, 800, 932
MaeII ACGT 3 cut(s) 69, 853, 891
MaeIII GTNAC 1 cut(s) 854
MalI GATC 4 cut(s) 433, 537, 666, 711
MboI GATC 4 cut(s) 431, 535, 664, 709
MboII GAAGA 6 cut(s) 398, 611, 622, 791, 824, 938
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 2 cut(s) 684, 794
MlsI TGGCCA 1 cut(s) 637
MluCI AATT 7 cut(s) 25, 83, 141, 653, 673, 701, 744
MluNI TGGCCA 1 cut(s) 637
MlyI GAGTC 2 cut(s) 380, 494
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 8 cut(s) 166, 199, 382, 449, 642, 820, 906, 934
Mox20I TGGCCA 1 cut(s) 637
Mph1103I ATGCAT 1 cut(s) 46
MroXI GAANNNNTTC 2 cut(s) 673, 783
MscI TGGCCA 1 cut(s) 637
MseI TTAA 4 cut(s) 354, 509, 524, 762
MslI CAYNNNNRTG 2 cut(s) 401, 718
Msp20I TGGCCA 1 cut(s) 637
MspCI CTTAAG 1 cut(s) 353
MspI CCGG 2 cut(s) 254, 539
MspR9I CCNGG 1 cut(s) 207
MssI GTTTAAAC 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 311
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 821
MwoI GCNNNNNNNGC 3 cut(s) 346, 355, 539
NcoI CCATGG 1 cut(s) 719
NdeI CATATG 2 cut(s) 96, 382
NdeII GATC 4 cut(s) 431, 535, 664, 709
NheI GCTAGC 1 cut(s) 799
NlaIII CATG 3 cut(s) 176, 400, 723
NlaIV GGNNCC 2 cut(s) 153, 751
NmuCI GTSAC 1 cut(s) 854
NsiI ATGCAT 1 cut(s) 46
NspV TTCGAA 1 cut(s) 597
PceI AGGCCT 1 cut(s) 730
PciSI GCTCTTC 1 cut(s) 921
PcsI WCGNNNNNNNCGW 1 cut(s) 888
PctI GAATGC 1 cut(s) 311
PdmI GAANNNNTTC 2 cut(s) 673, 783
PfeI GAWTC 5 cut(s) 210, 215, 515, 583, 669
PkrI GCNGC 4 cut(s) 246, 351, 775, 849
Ple19I CGATCG 1 cut(s) 667
PleI GAGTC 2 cut(s) 379, 493
PmeI GTTTAAAC 1 cut(s) 525
PpsI GAGTC 2 cut(s) 379, 493
PshBI ATTAAT 1 cut(s) 762
Psp6I CCWGG 1 cut(s) 205
PspFI CCCAGC 1 cut(s) 480
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 153, 751
PspPI GGNCC 1 cut(s) 749
PsuI RGATCY 1 cut(s) 431
PvuI CGATCG 1 cut(s) 667
RsaI GTAC 2 cut(s) 204, 617
RsaNI GTAC 2 cut(s) 203, 616
RseI CAYNNNNRTG 2 cut(s) 401, 718
SapI GCTCTTC 1 cut(s) 921
SaqAI TTAA 4 cut(s) 354, 509, 524, 762
SatI GCNGC 4 cut(s) 245, 350, 774, 848
Sau3AI GATC 4 cut(s) 431, 535, 664, 709
Sau96I GGNCC 1 cut(s) 749
ScaI AGTACT 1 cut(s) 617
SchI GAGTC 2 cut(s) 380, 494
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 2 cut(s) 684, 794
SfaNI GCATC 4 cut(s) 31, 53, 336, 439
SfuI TTCGAA 1 cut(s) 597
SinI GGWCC 1 cut(s) 749
SmiMI CAYNNNNRTG 2 cut(s) 401, 718
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 7 cut(s) 25, 83, 141, 653, 673, 701, 744
SseBI AGGCCT 1 cut(s) 730
SspMI CTAG 3 cut(s) 297, 800, 932
StuI AGGCCT 1 cut(s) 730
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 2 cut(s) 231, 719
TaaI ACNGT 3 cut(s) 623, 660, 833
TaiI ACGT 3 cut(s) 72, 856, 894
TaqI TCGA 5 cut(s) 213, 407, 597, 663, 667
TasI AATT 7 cut(s) 25, 83, 141, 653, 673, 701, 744
TatI WGTACW 1 cut(s) 615
TfiI GAWTC 5 cut(s) 210, 215, 515, 583, 669
Tru1I TTAA 4 cut(s) 354, 509, 524, 762
Tru9I TTAA 4 cut(s) 354, 509, 524, 762
TscAI CASTG 2 cut(s) 328, 712
TseFI GTSAC 1 cut(s) 854
TseI GCWGC 4 cut(s) 244, 349, 773, 847
Tsp45I GTSAC 1 cut(s) 854
TspDTI ATGAA 5 cut(s) 105, 189, 399, 432, 486
TspRI CASTG 2 cut(s) 328, 712
Vha464I CTTAAG 1 cut(s) 353
VneI GTGCAC 1 cut(s) 790
VpaK11BI GGWCC 1 cut(s) 749
VspI ATTAAT 1 cut(s) 762
XagI CCTNNNNNAGG 1 cut(s) 160
XapI RAATTY 1 cut(s) 25
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 492
XmnI GAANNNNTTC 2 cut(s) 673, 783
XspI CTAG 3 cut(s) 297, 800, 932
ZrmI AGTACT 1 cut(s) 617
Zsp2I ATGCAT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.