Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003821

Physical Location & Seq

Genomic Coordinates Reverse (-)

21053232 .. 21058283

Gene Structure 5052 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1134400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2859 bp

ATGGAGAGCTTTCGGCCTCGGAGGTCTAAGGTGACGAGCATTGCTGAGAGGAGCGCCGTCGGAGGACGATTCGCCTCCGCTGAGCGAGCTCACAGAAGAGGAAACAGGCAGGTTCTGAAACAGAGAGACTTTCGGAAATTGGCCTCAGATTCGAGTTCTTGCAGTAGTGGCTCTACCGGCGAAGACTGGTTCCCCTCTGAATTGGGCAGGAGATCTTCACAGCAAGCTGTTGGAACTCCAATAAAGAAGTTATTGGCTGAGGAGATGGTGAGAGAAACCGAACCTAGAAGGAGATCACCAAGCTTAATTGCCAAATTGATGGGTCTTGATGGGCTGCCACCTCAGCAGCCTGCTGATAGACAATCAAAGGGCATTTCAGAGAATTATCTTCAGAAGAAAAGATCGGTAGAGAAAGAGCACAGAAGCAGTATGTCTCATGACCGCCATTCATCTAGGAAGAACTCAAGGGTGCAGCAGGAGTTTAAGGATGTGTTTGAAGTTTATGAACCTGCAAGGATTTACTCATCTCGAGGAAGTGCTAACCCAAAGCTCAGTGATGCTGAAATGGCTTTTGTTCGACAGAAATTCATGGATGCAAAACGCCTTTCAACAGATGAGAGGCTACAGGATTCAAAGGAGTTCCATGATGTGCTTGAGGTGCTGGAATCTAACAAGGATATTCTATTGAAATTCCTTCAGCAACCAGACTCATTGTTCACAAAACATATGCATGATCTGCAAGGTGGTCCTCAATCCCTTTGTGGTCGCATAGCATCTTTGAAGCCATCAGAAGCTCAAAAGTATGAAAACATTGACCTTGGCTTCACATCAGCAAGAGAGCAGAAGCATCGCTGTAAATCTCCTCAGAAGCATCGGGATTCTTTTTCTAGCCACTCTGACAGTAGACATCCTCTTGAATCATCACTAAACCGACCAGAAGTGAAAATTGAATCTGCCATTCATCCTACAATTCATCCTACAAGGATAGTTGTTCTGAAACCAAACCTTGGGAAGGTGCTGAATGCTCCCAAAACATCACCTTGTTCTCCTCGTGCTTCTATGTTAGATGGTGGCAAACACGCTGAATTTCTGAGCATTAGAAACAGGGAGGCAGAATCATGTGGAAGGAAAAACGTCCAAGATAATGATGGGCATCTGAGACACAAGTCTAGAGAATCTAGAGAAATTGCTAAGGAAATCACAAGGAAAATGAGAGAAATAACTTTAGCAGTAATTCTTTCTTGTAGCATGTCTGAGAATGAATCTGCAAACGAGTCAGAGAGGATGTCGGTGGCTTCCAGGCATTCCTTTCACATAAGTAACCACTCCAGGCCTTCATCGTCGTGCTCTACTGAATCATCTGTGAGTAGAGAGGCCAAGAAGAGGCTCTCAGAGAGGTGGAAACTGACACACAAGTCCCAAGAAATGGGACCTGTGAGCAGGGGCAGCACACTTGGTGAAATGCTTGCTGTCCCAGACAAGGAAACGCGAGCTGCTAATTTGAATGCCATGATTGGTGAGGCAGGATTCAGAGATACATTTTCTACTGAAGGGGGTGGACCTCTGGGTATCAGCAGTAGGGATGGTTGGAAGGATGGATGCATCAATAGTTTATCAAGATCAAAATCTCTTCCTTCTTCATCTAGTGTGTTTGGAAGTTTTAAAACAAGTATGCGGTGTGAAACTGTACATAATGACAAGTATCCGATGCCAAAGGATACAGTCCCGCACGAAAGACACCGTATAGTAACTGGTAATCTTGATCTTAGAGAAGGTGTTCACAAACAGTCTAGATCCAGAAACAAAACATCTTATTCGTCTCCCTCAAGTAGGGAAGCTCTTGACATTTCACCTGAAACTCATACTACTGAGAATAAAGATAGAACTGACTTGGAAGAGAACAATCAAACCCAGAAGAATGTTGCAGTCTTTGAGTCACCACCTAGTAATGCTATGGATGCAAGTCCAGTTTCGGCAAATTTGGTGGATGTGGATGCATCCAAGCCTTCTGAAACTCCTGACGTGTTCCTTCCAGAATTATCATCTCATATGTCGGTAGAAGGCTATTCATCTGGTGGACACCAAGATAATGCAATTCCACAGGAACCCTCAATCAGGCCACCCCATGAACAGGCAGTCCCCTCTAACCAGTCTGTACCTGGAATCGAATCTCCTGCAAGCTCCAAGGAAGCTGAGCAGCCCAGTCCGGTTTCAGTTCTGGAAGTTCCTTTTACTGATGATGTGTCATCTAGTTCTGAATGCTTTGAGAGTGTCAGTGCTGACCTGCAAGGGCTTCGGATGCAGCTTAAGCTACTGAAGTTGGAGTCAGAACCATATGAAGAGGGACACATGGAAATGCAAGAGGATTTTTGTAAAGATGAAAAGAGCTGGGAGTCTGTATACTTGGCCGATACCTTAACTGAATCTGGTTTAAACAATGCTGATCCGGCTACCTTCTTGGCAATGTGGCACACCCCAGAATGTCCTGTGAATCCTTTACTATTCGAAGAACTGGAGAAGAAGTACTACGGTCAGACTTCTTGGCCAAAGCCCGAGAGGAAATTACTGTTCGATCGAATCAATTCAGGGCTCGTGGAGATGTTTGAGCAATTCACTGATCCATACCCATGGGTAAGGCCTGCGAGCAAAAGAATTGGTCCCAAGTGGATTAATCCAAGTGCAGCCGAAGATGTTCTGTGCACATTGCTAGCAAGCCAAGAAGAGAACGCGAACGAGGACAGTCTGGAGAAAGAGCTGCAACGTGACTTGCTGTGGCTGGATTTTGGAGACGAAATAGACGTAATAGGTAGGGAAGTAGAGAGGAAGTTGATAGAAGAGCTAGTAGCAGAGGTAGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3741 DUF4378 VARLMGL

Protein Analysis

953

Amino Acids

106.8

Weight (kDa)

6.3

Isoelectric Point (pI)

68.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VARLMGL	PF14383	93 - 116	1.2e-06	DUF761-associated sequence motif
DUF3741	PF12552	193 - 237	3.8e-21	Protein of unknown function (DUF3741)
DUF4378	PF14309	799 - 950	2.8e-34	Domain of unknown function (DUF4378)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003821)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53540 AT3G53540
fragaria_vesca	FvH4_6g14620 FvH4_6g14620 FvH4_6g14620 FvH4_6g14620 FvH4_6g14620 FvH4_6g14620 FvH4_6g14620 FvH4_6g14620
malus_domestica	MD04G1118500.v1.1 MD12G1134100.v1.1 MD12G1134200.v1.1 MD12G1134300.v1.1 MD12G1134400.v1.1
prunus_persica	Prupe.6G247900_v2.0.a1 Prupe.6G247900_v2.0.a1
pyrus_communis	pycom04g10820 pycom12g12970
rosa_chinensis	RchiOBHm_Chr3g0467721
rosa_laevigata	RLG00000024478
rosa_multiflora	Rmu_co8048390.1_g000001 Rmu_co8092630.1_g000001 Rmu_co8309069.1_g000001 Rmu_sc0001793.1_g000010 Rmu_sc0013459.1_g000002
rosa_roxburghii	Rroxscaffold_6G00413480
rosa_rugosa	Rorug03G0089200 Rorug03G0089200
rosa_samantha	Rh3AG137700 Rh3BG159000 Rh3CG158900 Rh3DG159500
rosa_wichuraiana	Rw3G013040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1414
Acc36I	ACCTGC	3 cut(s)	100, 517, 2292
AccB7I	CCANNNNNTGG	2 cut(s)	1007, 1426
AccI	GTMKAC	2 cut(s)	904, 2400
AccII	CGCG	2 cut(s)	1489, 2729
AciI	CCGC	4 cut(s)	78, 442, 1675, 1727
AclWI	GGATC	3 cut(s)	1788, 2438, 2612
AcoI	YGGCCR	2 cut(s)	2406, 2543
AcsI	RAATTY	4 cut(s)	584, 689, 1085, 1978
AcuI	CTGAAG	4 cut(s)	374, 680, 1569, 2336
AdeI	CACNNNGTG	1 cut(s)	1457
AfaI	GTAC	3 cut(s)	1689, 2157, 2525
AfiI	CCNNNNNNNGG	8 cut(s)	1007, 1012, 1383, 1426, 1480, 1830, 2115, 2131
AflII	CTTAAG	1 cut(s)	2306
AflIII	ACRYGT	1 cut(s)	2022
AgsI	TTSAA	8 cut(s)	497, 609, 633, 688, 781, 917, 950, 1504
AjiI	CACGTC	1 cut(s)	2023
AjnI	CCWGG	3 cut(s)	1298, 1328, 2158
AloI	GAACNNNNNNTCC	2 cut(s)	2121, 2153
Alw21I	GWGCWC	4 cut(s)	91, 420, 1349, 2702
Alw26I	GTCTC	5 cut(s)	120, 438, 1153, 1824, 2781
Alw44I	GTGCAC	1 cut(s)	2698
AlwI	GGATC	3 cut(s)	1788, 2438, 2612
AlwNI	CAGNNNCTG	1 cut(s)	115
Ama87I	CYCGRG	2 cut(s)	528, 2552
AoxI	GGCC	8 cut(s)	14, 141, 1331, 1374, 2117, 2406, 2543, 2636
ApaLI	GTGCAC	1 cut(s)	2698
ApeKI	GCWGC	9 cut(s)	334, 346, 472, 1446, 1493, 2197, 2302, 2681, 2755
ApoI	RAATTY	4 cut(s)	584, 689, 1085, 1978
AseI	ATTAAT	1 cut(s)	2670
Asp700I	GAANNNNTTC	3 cut(s)	1776, 2581, 2691
AspLEI	GCGC	1 cut(s)	56
AspS9I	GGNCC	4 cut(s)	746, 1430, 1559, 2657
AsuHPI	GGTGA	8 cut(s)	43, 280, 288, 1029, 1469, 1529, 1842, 1929
AsuII	TTCGAA	1 cut(s)	2505
AsuNHI	GCTAGC	1 cut(s)	2707
AvaI	CYCGRG	2 cut(s)	528, 2552
AvaII	GGWCC	4 cut(s)	746, 1430, 1559, 2657
BaeGI	GKGCMC	1 cut(s)	2702
BalI	TGGCCA	1 cut(s)	2545
BanII	GRGCYC	2 cut(s)	91, 2592
BarI	GAAGNNNNNNTAC	2 cut(s)	2510, 2542
BauI	CACGAG	2 cut(s)	1050, 2591
BbsI	GAAGAC	1 cut(s)	189
Bbv12I	GWGCWC	4 cut(s)	91, 420, 1349, 2702
BbvCI	CCTCAGC	2 cut(s)	258, 342
BbvI	GCAGC	9 cut(s)	321, 358, 484, 1458, 1480, 2209, 2314, 2693, 2742
BccI	CCATC	8 cut(s)	259, 313, 323, 793, 1061, 1142, 1577, 1589
BceAI	ACGGC	1 cut(s)	41
BcgI	CGANNNNNNTGC	6 cut(s)	830, 864, 2156, 2190, 2276, 2310
BciT130I	CCWGG	3 cut(s)	1300, 1330, 2160
BciVI	GTATCC	2 cut(s)	1711, 1713
BcoDI	GTCTC	5 cut(s)	120, 438, 1153, 1824, 2781
BfmI	CTRYAG	1 cut(s)	623
BfoI	RGCGCY	1 cut(s)	57
BfrI	CTTAAG	1 cut(s)	2306
BfuAI	ACCTGC	3 cut(s)	100, 517, 2292
BfuI	GTATCC	2 cut(s)	1711, 1713
BglII	AGATCT	1 cut(s)	212
BisI	GCNGC	9 cut(s)	335, 347, 473, 1447, 1494, 2198, 2303, 2682, 2756
BlpI	GCTNAGC	2 cut(s)	81, 2193
BlsI	GCNGC	9 cut(s)	336, 348, 474, 1448, 1495, 2199, 2304, 2683, 2757
BmcAI	AGTACT	1 cut(s)	2525
Bme1390I	CCNGG	3 cut(s)	1300, 1330, 2160
Bme18I	GGWCC	4 cut(s)	746, 1430, 1559, 2657
BmeT110I	CYCGRG	2 cut(s)	528, 2552
BmgBI	CACGTC	1 cut(s)	2023
BmgT120I	GGNCC	4 cut(s)	746, 1430, 1559, 2657
BmiI	GGNNCC	4 cut(s)	191, 1431, 2106, 2659
BmrFI	CCNGG	3 cut(s)	1300, 1330, 2160
BmrI	ACTGGG	1 cut(s)	2196
BmtI	GCTAGC	1 cut(s)	2711
BmuI	ACTGGG	1 cut(s)	2196
BoxI	GACNNNNGTC	1 cut(s)	1165
BpiI	GAAGAC	1 cut(s)	189
BpmI	CTGGAG	3 cut(s)	1312, 2534, 2765
Bpu10I	CCTNAGC	3 cut(s)	258, 342, 1191
Bpu1102I	GCTNAGC	2 cut(s)	81, 2193
Bpu14I	TTCGAA	1 cut(s)	2505
BpuEI	CTTGAG	3 cut(s)	448, 674, 1810
BsaBI	GATNNNNATC	2 cut(s)	1152, 1624
BsaJI	CCNNGG	5 cut(s)	17, 817, 1006, 2184, 2627
BsaWI	WCCGGW	1 cut(s)	2206
BsaXI	ACNNNNNCTCC	2 cut(s)	2507, 2537
Bsc4I	CCNNNNNNNGG	8 cut(s)	1007, 1012, 1383, 1426, 1480, 1830, 2115, 2131
Bse118I	RCCGGY	1 cut(s)	176
Bse1I	ACTGG	6 cut(s)	191, 1756, 1967, 2149, 2202, 2517
Bse3DI	GCAATG	3 cut(s)	39, 2469, 2702
Bse8I	GATNNNNATC	2 cut(s)	1152, 1624
BseBI	CCWGG	3 cut(s)	1300, 1330, 2160
BseDI	CCNNGG	5 cut(s)	17, 817, 1006, 2184, 2627
BseJI	GATNNNNATC	2 cut(s)	1152, 1624
BseLI	CCNNNNNNNGG	8 cut(s)	1007, 1012, 1383, 1426, 1480, 1830, 2115, 2131
BseMI	GCAATG	3 cut(s)	39, 2469, 2702
BseNI	ACTGG	6 cut(s)	191, 1756, 1967, 2149, 2202, 2517
BseRI	GAGGAG	4 cut(s)	64, 275, 852, 1038
BseSI	GKGCMC	1 cut(s)	2702
BseXI	GCAGC	9 cut(s)	321, 358, 484, 1458, 1480, 2209, 2314, 2693, 2742
BseYI	CCCAGC	1 cut(s)	2388
BsgI	GTGCAG	2 cut(s)	491, 2700
Bsh1236I	CGCG	2 cut(s)	1489, 2729
Bsh1285I	CGRYCG	1 cut(s)	2575
BshFI	GGCC	8 cut(s)	16, 143, 1333, 1376, 2119, 2408, 2545, 2638
BsiEI	CGRYCG	1 cut(s)	2575
BsiHKAI	GWGCWC	4 cut(s)	91, 420, 1349, 2702
BsiHKCI	CYCGRG	2 cut(s)	528, 2552
BsiSI	CCGG	3 cut(s)	177, 2207, 2447
BslFI	GGGAC	7 cut(s)	1402, 1443, 1457, 1709, 2123, 2358, 2643
BslI	CCNNNNNNNGG	8 cut(s)	1007, 1012, 1383, 1426, 1480, 1830, 2115, 2131
BsmAI	GTCTC	5 cut(s)	120, 438, 1153, 1824, 2781
BsmBI	CGTCTC	2 cut(s)	1824, 2781
BsmFI	GGGAC	7 cut(s)	1402, 1443, 1457, 1709, 2123, 2358, 2643
BsmI	GAATGC	4 cut(s)	1027, 1303, 1510, 2264
BsnI	GGCC	8 cut(s)	16, 143, 1333, 1376, 2119, 2408, 2545, 2638
BsoBI	CYCGRG	2 cut(s)	528, 2552
Bsp119I	TTCGAA	1 cut(s)	2505
Bsp1286I	GDGCHC	5 cut(s)	91, 420, 1349, 2592, 2702
Bsp1407I	TGTACA	1 cut(s)	1687
Bsp1720I	GCTNAGC	2 cut(s)	81, 2193
Bsp19I	CCATGG	1 cut(s)	2627
BspACI	CCGC	4 cut(s)	78, 442, 1675, 1727
BspANI	GGCC	8 cut(s)	16, 143, 1333, 1376, 2119, 2408, 2545, 2638
BspFNI	CGCG	2 cut(s)	1489, 2729
BspHI	TCATGA	1 cut(s)	436
BspLI	GGNNCC	4 cut(s)	191, 1431, 2106, 2659
BspMI	ACCTGC	3 cut(s)	100, 517, 2292
BspOI	GCTAGC	1 cut(s)	2711
BspPI	GGATC	3 cut(s)	1788, 2438, 2612
BspQI	GCTCTTC	1 cut(s)	2829
BspT104I	TTCGAA	1 cut(s)	2505
BspTI	CTTAAG	1 cut(s)	2306
BsrDI	GCAATG	3 cut(s)	39, 2469, 2702
BsrFI	RCCGGY	1 cut(s)	176
BsrGI	TGTACA	1 cut(s)	1687
BsrI	ACTGG	6 cut(s)	191, 1756, 1967, 2149, 2202, 2517
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	5 cut(s)	17, 817, 1006, 2184, 2627
BssNAI	GTATAC	1 cut(s)	2401
BssSI	CACGAG	2 cut(s)	1050, 2591
BssT1I	CCWWGG	4 cut(s)	817, 1006, 2184, 2627
Bst1107I	GTATAC	1 cut(s)	2401
Bst2BI	CACGAG	2 cut(s)	1050, 2591
Bst2UI	CCWGG	3 cut(s)	1300, 1330, 2160
Bst4CI	ACNGT	8 cut(s)	902, 1687, 1723, 1742, 1788, 2531, 2568, 2741
Bst6I	CTCTTC	7 cut(s)	91, 1376, 1635, 1890, 2334, 2715, 2829
BstAFI	CTTAAG	1 cut(s)	2306
BstAPI	GCANNNNNTGC	1 cut(s)	736
BstAUI	TGTACA	1 cut(s)	1687
BstBI	TTCGAA	1 cut(s)	2505
BstDSI	CCRYGG	1 cut(s)	2627
BstENI	CCTNNNNNAGG	3 cut(s)	1010, 1828, 2113
BstFNI	CGCG	2 cut(s)	1489, 2729
BstH2I	RGCGCY	1 cut(s)	57
BstHHI	GCGC	1 cut(s)	56
BstMAI	GTCTC	5 cut(s)	120, 438, 1153, 1824, 2781
BstMCI	CGRYCG	1 cut(s)	2575
BstNI	CCWGG	3 cut(s)	1300, 1330, 2160
BstNSI	RCATGY	1 cut(s)	1252
BstPAI	GACNNNNGTC	1 cut(s)	1165
BstSCI	CCNGG	3 cut(s)	1298, 1328, 2158
BstSFI	CTRYAG	1 cut(s)	623
BstSLI	GKGCMC	1 cut(s)	2702
BstUI	CGCG	2 cut(s)	1489, 2729
BstV1I	GCAGC	9 cut(s)	321, 358, 484, 1458, 1480, 2209, 2314, 2693, 2742
BstV2I	GAAGAC	1 cut(s)	189
BstX2I	RGATCY	2 cut(s)	212, 1793
BstXI	CCANNNNNNTGG	2 cut(s)	319, 2628
BstYI	RGATCY	2 cut(s)	212, 1793
BstZ17I	GTATAC	1 cut(s)	2401
BsuI	GTATCC	2 cut(s)	1711, 1713
BsuRI	GGCC	8 cut(s)	16, 143, 1333, 1376, 2119, 2408, 2545, 2638
BtgI	CCRYGG	1 cut(s)	2627
BtgZI	GCGATG	1 cut(s)	833
BtrI	CACGTC	1 cut(s)	2023
BtsIMutI	CAGTG	3 cut(s)	559, 2281, 2613
BveI	ACCTGC	3 cut(s)	100, 517, 2292
CaiI	CAGNNNCTG	1 cut(s)	115
CciI	TCATGA	1 cut(s)	436
CfoI	GCGC	1 cut(s)	56
Cfr10I	RCCGGY	1 cut(s)	176
Cfr13I	GGNCC	4 cut(s)	746, 1430, 1559, 2657
Csp6I	GTAC	3 cut(s)	1688, 2156, 2524
CspCI	CAANNNNNGTGG	2 cut(s)	1965, 2000
CviQI	GTAC	3 cut(s)	1688, 2156, 2524
DraI	TTTAAA	2 cut(s)	1663, 2433
DraIII	CACNNNGTG	1 cut(s)	1457
DrdI	GACNNNNNNGTC	1 cut(s)	1414
DseDI	GACNNNNNNGTC	1 cut(s)	1414
EaeI	YGGCCR	2 cut(s)	2406, 2543
Eam1104I	CTCTTC	7 cut(s)	91, 1376, 1635, 1890, 2334, 2715, 2829
EarI	CTCTTC	7 cut(s)	91, 1376, 1635, 1890, 2334, 2715, 2829
Ecl136II	GAGCTC	1 cut(s)	89
Eco130I	CCWWGG	4 cut(s)	817, 1006, 2184, 2627
Eco147I	AGGCCT	2 cut(s)	1333, 2638
Eco24I	GRGCYC	2 cut(s)	91, 2592
Eco47I	GGWCC	4 cut(s)	746, 1430, 1559, 2657
Eco53kI	GAGCTC	1 cut(s)	89
Eco57I	CTGAAG	4 cut(s)	374, 680, 1569, 2336
Eco88I	CYCGRG	2 cut(s)	528, 2552
EcoICRI	GAGCTC	1 cut(s)	89
EcoNI	CCTNNNNNAGG	3 cut(s)	1010, 1828, 2113
EcoO109I	RGGNCCY	1 cut(s)	1430
EcoRII	CCWGG	3 cut(s)	1298, 1328, 2158
EcoT14I	CCWWGG	4 cut(s)	817, 1006, 2184, 2627
EcoT22I	ATGCAT	3 cut(s)	732, 1604, 1999
EcoT38I	GRGCYC	2 cut(s)	91, 2592
ErhI	CCWWGG	4 cut(s)	817, 1006, 2184, 2627
Esp3I	CGTCTC	2 cut(s)	1824, 2781
FaqI	GGGAC	7 cut(s)	1402, 1443, 1457, 1709, 2123, 2358, 2643
FauI	CCCGC	1 cut(s)	1734
FauNDI	CATATG	3 cut(s)	726, 2049, 2335
FblI	GTMKAC	2 cut(s)	904, 2400
Fnu4HI	GCNGC	9 cut(s)	335, 347, 473, 1447, 1494, 2198, 2303, 2682, 2756
FriOI	GRGCYC	2 cut(s)	91, 2592
Fsp4HI	GCNGC	9 cut(s)	335, 347, 473, 1447, 1494, 2198, 2303, 2682, 2756
GlaI	GCGC	1 cut(s)	55
GluI	GCNGC	9 cut(s)	335, 347, 473, 1447, 1494, 2198, 2303, 2682, 2756
GsaI	CCCAGC	1 cut(s)	2392
GsuI	CTGGAG	3 cut(s)	1312, 2534, 2765
HaeII	RGCGCY	1 cut(s)	57
HaeIII	GGCC	8 cut(s)	16, 143, 1333, 1376, 2119, 2408, 2545, 2638
HapII	CCGG	3 cut(s)	177, 2207, 2447
HhaI	GCGC	1 cut(s)	56
Hin6I	GCGC	1 cut(s)	54
HinP1I	GCGC	1 cut(s)	54
HindIII	AAGCTT	1 cut(s)	301
HpaII	CCGG	3 cut(s)	177, 2207, 2447
HphI	GGTGA	8 cut(s)	43, 280, 288, 1029, 1469, 1529, 1842, 1929
Hpy166II	GTNNAC	7 cut(s)	717, 905, 1559, 1780, 2078, 2401, 2700
Hpy8I	GTNNAC	7 cut(s)	717, 905, 1559, 1780, 2078, 2401, 2700
Hpy99I	CGWCG	2 cut(s)	62, 1345
HpyCH4III	ACNGT	8 cut(s)	902, 1687, 1723, 1742, 1788, 2531, 2568, 2741
HpyCH4IV	ACGT	4 cut(s)	1134, 2022, 2761, 2799
HpySE526I	ACGT	4 cut(s)	1134, 2022, 2761, 2799
HspAI	GCGC	1 cut(s)	54
LguI	GCTCTTC	1 cut(s)	2829
LmnI	GCTCC	3 cut(s)	51, 1030, 2186
Lsp1109I	GCAGC	9 cut(s)	321, 358, 484, 1458, 1480, 2209, 2314, 2693, 2742
MaeII	ACGT	4 cut(s)	1134, 2022, 2761, 2799
MaeIII	GTNAC	5 cut(s)	31, 1319, 1747, 1935, 2762
MflI	RGATCY	2 cut(s)	212, 1793
MhlI	GDGCHC	5 cut(s)	91, 420, 1349, 2592, 2702
MlsI	TGGCCA	1 cut(s)	2545
MluNI	TGGCCA	1 cut(s)	2545
MlyI	GAGTC	5 cut(s)	701, 1283, 1943, 2333, 2402
MmeI	TCCRAC	4 cut(s)	40, 211, 1568, 2301
Mox20I	TGGCCA	1 cut(s)	2545
Mph1103I	ATGCAT	3 cut(s)	732, 1604, 1999
MroXI	GAANNNNTTC	3 cut(s)	1776, 2581, 2691
MscI	TGGCCA	1 cut(s)	2545
MseI	TTAA	7 cut(s)	305, 483, 1662, 2307, 2417, 2432, 2670
MslI	CAYNNNNRTG	4 cut(s)	729, 1342, 2354, 2626
Msp20I	TGGCCA	1 cut(s)	2545
MspA1I	CMGCKG	1 cut(s)	80
MspCI	CTTAAG	1 cut(s)	2306
MspI	CCGG	3 cut(s)	177, 2207, 2447
MspR9I	CCNGG	3 cut(s)	1300, 1330, 2160
MssI	GTTTAAAC	1 cut(s)	2433
Mva1269I	GAATGC	4 cut(s)	1027, 1303, 1510, 2264
MvaI	CCWGG	3 cut(s)	1300, 1330, 2160
MvnI	CGCG	2 cut(s)	1489, 2729
NcoI	CCATGG	1 cut(s)	2627
NdeI	CATATG	3 cut(s)	726, 2049, 2335
NheI	GCTAGC	1 cut(s)	2707
NlaIV	GGNNCC	4 cut(s)	191, 1431, 2106, 2659
NmuCI	GTSAC	3 cut(s)	31, 1935, 2762
NsiI	ATGCAT	3 cut(s)	732, 1604, 1999
NspI	RCATGY	1 cut(s)	1252
NspV	TTCGAA	1 cut(s)	2505
PaeR7I	CTCGAG	1 cut(s)	528
PagI	TCATGA	1 cut(s)	436
PceI	AGGCCT	2 cut(s)	1333, 2638
PciSI	GCTCTTC	1 cut(s)	2829
PcsI	WCGNNNNNNNCGW	1 cut(s)	2796
PctI	GAATGC	4 cut(s)	1027, 1303, 1510, 2264
PdmI	GAANNNNTTC	3 cut(s)	1776, 2581, 2691
PflMI	CCANNNNNTGG	2 cut(s)	1007, 1426
PkrI	GCNGC	9 cut(s)	336, 348, 474, 1448, 1495, 2199, 2304, 2683, 2757
Ple19I	CGATCG	1 cut(s)	2575
PleI	GAGTC	5 cut(s)	701, 1282, 1942, 2332, 2401
PmeI	GTTTAAAC	1 cut(s)	2433
PpsI	GAGTC	5 cut(s)	701, 1282, 1942, 2332, 2401
PpuMI	RGGWCCY	1 cut(s)	1430
PshAI	GACNNNNGTC	1 cut(s)	1165
PshBI	ATTAAT	1 cut(s)	2670
Psp124BI	GAGCTC	1 cut(s)	91
Psp5II	RGGWCCY	1 cut(s)	1430
Psp6I	CCWGG	3 cut(s)	1298, 1328, 2158
PspFI	CCCAGC	1 cut(s)	2388
PspGI	CCWGG	3 cut(s)	1298, 1328, 2158
PspN4I	GGNNCC	4 cut(s)	191, 1431, 2106, 2659
PspPI	GGNCC	4 cut(s)	746, 1430, 1559, 2657
PspPPI	RGGWCCY	1 cut(s)	1430
PstNI	CAGNNNCTG	1 cut(s)	115
PsuI	RGATCY	2 cut(s)	212, 1793
PvuI	CGATCG	1 cut(s)	2575
RsaI	GTAC	3 cut(s)	1689, 2157, 2525
RsaNI	GTAC	3 cut(s)	1688, 2156, 2524
RseI	CAYNNNNRTG	4 cut(s)	729, 1342, 2354, 2626
SacI	GAGCTC	1 cut(s)	91
SapI	GCTCTTC	1 cut(s)	2829
SaqAI	TTAA	7 cut(s)	305, 483, 1662, 2307, 2417, 2432, 2670
SatI	GCNGC	9 cut(s)	335, 347, 473, 1447, 1494, 2198, 2303, 2682, 2756
Sau96I	GGNCC	4 cut(s)	746, 1430, 1559, 2657
ScaI	AGTACT	1 cut(s)	2525
SchI	GAGTC	5 cut(s)	701, 1283, 1943, 2333, 2402
ScrFI	CCNGG	3 cut(s)	1300, 1330, 2160
SduI	GDGCHC	5 cut(s)	91, 420, 1349, 2592, 2702
SfcI	CTRYAG	1 cut(s)	623
Sfr274I	CTCGAG	1 cut(s)	528
SfuI	TTCGAA	1 cut(s)	2505
SinI	GGWCC	4 cut(s)	746, 1430, 1559, 2657
SlaI	CTCGAG	1 cut(s)	528
SmiMI	CAYNNNNRTG	4 cut(s)	729, 1342, 2354, 2626
SmlI	CTYRAG	5 cut(s)	463, 528, 653, 1825, 2306
SmoI	CTYRAG	5 cut(s)	463, 528, 653, 1825, 2306
SseBI	AGGCCT	2 cut(s)	1333, 2638
SsiI	CCGC	4 cut(s)	78, 442, 1675, 1727
SstI	GAGCTC	1 cut(s)	91
StuI	AGGCCT	2 cut(s)	1333, 2638
StyD4I	CCNGG	3 cut(s)	1298, 1328, 2158
StyI	CCWWGG	4 cut(s)	817, 1006, 2184, 2627
TaaI	ACNGT	8 cut(s)	902, 1687, 1723, 1742, 1788, 2531, 2568, 2741
TaiI	ACGT	4 cut(s)	1137, 2025, 2764, 2802
TaqI	TCGA	7 cut(s)	152, 529, 577, 2166, 2505, 2571, 2575
TatI	WGTACW	2 cut(s)	1687, 2523
Tru1I	TTAA	7 cut(s)	305, 483, 1662, 2307, 2417, 2432, 2670
Tru9I	TTAA	7 cut(s)	305, 483, 1662, 2307, 2417, 2432, 2670
TscAI	CASTG	3 cut(s)	559, 2281, 2620
TseFI	GTSAC	3 cut(s)	31, 1935, 2762
TseI	GCWGC	9 cut(s)	334, 346, 472, 1446, 1493, 2197, 2302, 2681, 2755
Tsp45I	GTSAC	3 cut(s)	31, 1935, 2762
TspRI	CASTG	3 cut(s)	559, 2281, 2620
Van91I	CCANNNNNTGG	2 cut(s)	1007, 1426
Vha464I	CTTAAG	1 cut(s)	2306
VneI	GTGCAC	1 cut(s)	2698
VpaK11BI	GGWCC	4 cut(s)	746, 1430, 1559, 2657
VspI	ATTAAT	1 cut(s)	2670
XagI	CCTNNNNNAGG	3 cut(s)	1010, 1828, 2113
XapI	RAATTY	4 cut(s)	584, 689, 1085, 1978
XbaI	TCTAGA	3 cut(s)	1169, 1178, 1790
XceI	RCATGY	1 cut(s)	1252
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1145, 2156
XhoI	CTCGAG	1 cut(s)	528
XmiI	GTMKAC	2 cut(s)	904, 2400
XmnI	GAANNNNTTC	3 cut(s)	1776, 2581, 2691
ZrmI	AGTACT	1 cut(s)	2525
Zsp2I	ATGCAT	3 cut(s)	732, 1604, 1999

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.