Rorug03G0089200

Protein of unknown function (DUF3741)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003821
Physical Location & Seq
Reverse (-)
6953400 .. 6956175
2776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0089200.1

Sequence Viewer

Length: 1386 bp
ATGGCAAGCACTTCATCCAACACCCTTGAGGTCAATGTTAGCAGCATCAACTTTAAGACCTTAGTGATTGACAATGGTAGCGAACCGATCCCTCAATATCCTTTCAATGTTTTCTGTCCCAAGTCCCTCATGTTTGTGCTCCCTGAAGATCCCAAGGACTTGCTAGAAGTCTTGATGAAGGTCAATTCAACCCAAGGCATGTTTACGAGTCTCATCAAAATGTATGATGCTTTGTTAACCGATGGCCTCAAGCATCAGACCAGGGAGATTGATGCTCAAATCGACAAAGATGTGGTGTCTGAGGTGGTTGTCTACACAGGCATCATTGAGGAGTATGCAGACGCCGGTAAGACCAAGGAGGCGCTGACGGTGTTCAAGCGCATGTTACTCTTTGATGTCACCCCCAATGCTTACACTTACAGTGTGCTCATTAAGGCACTTGCTTCAGATCCAGACCCCAGTTCTGTCGGGCTTGCCAAGGATTTCGTGCTGGAAATGATGCACAAGGGAATGCAGCCCAATGCCAGGACATACACTGCCGTGTTTGAGGCATTTGCTGGGCATGAGAAGATGGAGCAGGCCAAGCAGTTTCTGGATGAGATAAAAGCTAATGGCTTTGTGGCTGACAGGAAGGCAATGAAGGCTATGAGACATGTTCTAAAGGGCAGTATAGGCCATGTGGTTAGAACCATAGCCGACATTCTCTTTGATGATCCAGAAGACTATTCAGACTACTCAGATGATGATGATGATGTTATAAAGAAGATTAGCAAAGAGATGGACTGGTCGAGGTTCCCTCCTGATAGCTATTTATCTTTCTGCAAGAAACAAATCATTGAGAATGACCCAGAAAACCCCAAAGATTTGGAAGATGTCTTTGACACAATGCGCAAGGCGGACCCTGTCACCTTCACTATGGCCAGAATGATCAACATGCTGAGGAAAGACCGATTTATGTCCCAGGCCGAAGAGATATTTGTCAAGATGGACTTGGATCGCATTATACCTGAAGTTGTGACACACACTGGTGTCATTGAGATCTATGCCAAGGCATACGAGGCCAAGGAGGCTCTCAAGGTGTTTGTGCGCATGTTATCCCTTGGTGTCACCCCCAATGCATACACTTACAGTGTTCTCATTAAGGGACTCGCTAAAGATCCCAACTTTCTTGGTGATGCCAAGAAGTACACTCTGGAAATGATGGATAAGGGAATGCAGCCCAATGCCAGGACCTACATCGCTGTGTTTAAGGCCTTTGCCAGGCTAAAGGATTTCAATGTGGAGAAGGGCAAGGAGTTTCTGAAGGAGATGAAAGCGAAGGGGTTTGTTCCTAAAAAGAAGGCTGTGATGGAGGTTTTGAAGGGAAAAAAAAGGACATGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378 VARLMGL

Protein Analysis

461

Amino Acids

52.21

Weight (kDa)

6.01

Isoelectric Point (pI)

26.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 95 - 147 1.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 102 - 148 3.9e-11 PPR repeat family
PPR_long PF17177 118 - 215 3.9e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 325 - 381 3.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 336 - 385 1.1e-11 PPR repeat family
TPR_24 PF23276 341 - 425 9e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 354 - 454 4.3e-07 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 758
Acc16I TGCGCA 2 cut(s) 890, 1088
AccI GTMKAC 1 cut(s) 312
AciI CCGC 1 cut(s) 896
AclWI GGATC 6 cut(s) 82, 143, 443, 707, 1002, 1151
AcoI YGGCCR 1 cut(s) 918
AcuI CTGAAG 4 cut(s) 165, 429, 1029, 1322
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 1 cut(s) 1187
AfiI CCNNNNNNNGG 3 cut(s) 525, 1227, 1260
AflIII ACRYGT 2 cut(s) 652, 1376
AgsI TTSAA 5 cut(s) 106, 189, 376, 1276, 1360
AjnI CCWGG 5 cut(s) 260, 524, 960, 1226, 1259
AleI CACNNNNGTG 2 cut(s) 539, 1026
AluBI AGCT 2 cut(s) 608, 807
AluI AGCT 2 cut(s) 608, 807
Alw21I GWGCWC 2 cut(s) 141, 429
Alw26I GTCTC 2 cut(s) 215, 643
AlwI GGATC 6 cut(s) 82, 143, 443, 707, 1002, 1151
AlwNI CAGNNNCTG 1 cut(s) 592
AoxI GGCC 7 cut(s) 244, 579, 673, 918, 963, 1059, 1251
ApeKI GCWGC 3 cut(s) 42, 514, 1216
ArsI GACNNNNNNTTYG 2 cut(s) 689, 721
AspLEI GCGC 4 cut(s) 364, 381, 891, 1089
AspS9I GGNCC 2 cut(s) 898, 1230
AsuHPI GGTGA 4 cut(s) 391, 898, 1099, 1184
AvaII GGWCC 2 cut(s) 898, 1230
BalI TGGCCA 1 cut(s) 920
BbsI GAAGAC 1 cut(s) 726
Bbv12I GWGCWC 2 cut(s) 141, 429
BbvCI CCTCAGC 1 cut(s) 938
BbvI GCAGC 3 cut(s) 54, 526, 1228
BccI CCATC 6 cut(s) 236, 565, 772, 979, 1195, 1342
BceAI ACGGC 1 cut(s) 524
BciT130I CCWGG 5 cut(s) 262, 526, 962, 1228, 1261
BclI TGATCA 1 cut(s) 927
BcoDI GTCTC 2 cut(s) 215, 643
BfaI CTAG 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 365
BglI GCCNNNNNGGC 1 cut(s) 1067
BglII AGATCT 1 cut(s) 1038
BisI GCNGC 3 cut(s) 43, 515, 1217
BlsI GCNGC 3 cut(s) 44, 516, 1218
Bme1390I CCNGG 5 cut(s) 262, 526, 962, 1228, 1261
Bme18I GGWCC 2 cut(s) 898, 1230
BmgT120I GGNCC 2 cut(s) 898, 1230
BmiI GGNNCC 2 cut(s) 794, 900
BmrFI CCNGG 5 cut(s) 262, 526, 962, 1228, 1261
BmrI ACTGGG 1 cut(s) 453
BmsI GCATC 7 cut(s) 54, 217, 262, 262, 330, 489, 1165
BmuI ACTGGG 1 cut(s) 453
BpiI GAAGAC 1 cut(s) 726
BplI GAGNNNNNCTC 2 cut(s) 781, 813
Bpu10I CCTNAGC 1 cut(s) 938
BpuEI CTTGAG 3 cut(s) 47, 233, 1058
BsaHI GRCGYC 1 cut(s) 342
BsaJI CCNNGG 9 cut(s) 153, 193, 261, 354, 477, 960, 1047, 1062, 1099
Bsc4I CCNNNNNNNGG 3 cut(s) 525, 1227, 1260
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 3 cut(s) 459, 788, 1030
Bse3DI GCAATG 1 cut(s) 642
BseBI CCWGG 5 cut(s) 262, 526, 962, 1228, 1261
BseDI CCNNGG 9 cut(s) 153, 193, 261, 354, 477, 960, 1047, 1062, 1099
BseGI GGATG 2 cut(s) 14, 601
BseLI CCNNNNNNNGG 3 cut(s) 525, 1227, 1260
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 3 cut(s) 291, 750, 929
BseNI ACTGG 3 cut(s) 459, 788, 1030
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 3 cut(s) 54, 526, 1228
BseYI CCCAGC 1 cut(s) 557
BshFI GGCC 7 cut(s) 246, 581, 675, 920, 965, 1061, 1253
BsiHKAI GWGCWC 2 cut(s) 141, 429
BsiSI CCGG 1 cut(s) 345
BslFI GGGAC 4 cut(s) 102, 109, 943, 1158
BslI CCNNNNNNNGG 3 cut(s) 525, 1227, 1260
BsmAI GTCTC 2 cut(s) 215, 643
BsmFI GGGAC 4 cut(s) 102, 109, 943, 1158
BsmI GAATGC 2 cut(s) 516, 1218
BsnI GGCC 7 cut(s) 246, 581, 675, 920, 965, 1061, 1253
Bsp1286I GDGCHC 2 cut(s) 141, 429
Bsp143I GATC 8 cut(s) 87, 148, 448, 712, 927, 994, 1038, 1156
BspACI CCGC 1 cut(s) 896
BspANI GGCC 7 cut(s) 246, 581, 675, 920, 965, 1061, 1253
BspCNI CTCAG 3 cut(s) 292, 749, 930
BspLI GGNNCC 2 cut(s) 794, 900
BspPI GGATC 6 cut(s) 82, 143, 443, 707, 1002, 1151
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 3 cut(s) 459, 788, 1030
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 9 cut(s) 153, 193, 261, 354, 477, 960, 1047, 1062, 1099
BssMI GATC 8 cut(s) 87, 148, 448, 712, 927, 994, 1038, 1156
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 7 cut(s) 153, 193, 354, 477, 1047, 1062, 1099
Bst2UI CCWGG 5 cut(s) 262, 526, 962, 1228, 1261
Bst4CI ACNGT 3 cut(s) 370, 422, 1130
Bst6I CTCTTC 1 cut(s) 963
BstACI GRCGYC 1 cut(s) 342
BstC8I GCNNGC 3 cut(s) 7, 474, 579
BstDEI CTNAG 4 cut(s) 61, 300, 736, 938
BstENI CCTNNNNNAGG 1 cut(s) 1258
BstF5I GGATG 2 cut(s) 14, 601
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 4 cut(s) 364, 381, 891, 1089
BstKTI GATC 8 cut(s) 90, 151, 451, 715, 930, 997, 1041, 1159
BstMAI GTCTC 2 cut(s) 215, 643
BstMBI GATC 8 cut(s) 87, 148, 448, 712, 927, 994, 1038, 1156
BstMWI GCNNNNNNNGC 5 cut(s) 583, 641, 672, 1058, 1067
BstNI CCWGG 5 cut(s) 262, 526, 962, 1228, 1261
BstNSI RCATGY 6 cut(s) 202, 385, 656, 937, 1093, 1380
BstSCI CCNGG 5 cut(s) 260, 524, 960, 1226, 1259
BstV1I GCAGC 3 cut(s) 54, 526, 1228
BstV2I GAAGAC 1 cut(s) 726
BstX2I RGATCY 4 cut(s) 148, 448, 1038, 1156
BstXI CCANNNNNNTGG 1 cut(s) 865
BstYI RGATCY 4 cut(s) 148, 448, 1038, 1156
BsuRI GGCC 7 cut(s) 246, 581, 675, 920, 965, 1061, 1253
BtgZI GCGATG 1 cut(s) 1222
BtsCI GGATG 2 cut(s) 14, 601
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 4 cut(s) 427, 534, 1023, 1135
Cac8I GCNNGC 3 cut(s) 7, 474, 579
CaiI CAGNNNCTG 1 cut(s) 592
CfoI GCGC 4 cut(s) 364, 381, 891, 1089
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 2 cut(s) 898, 1230
CseI GACGC 1 cut(s) 350
Csp6I GTAC 1 cut(s) 1186
CviAII CATG 9 cut(s) 130, 199, 382, 563, 653, 677, 934, 1090, 1377
CviQI GTAC 1 cut(s) 1186
DdeI CTNAG 4 cut(s) 61, 300, 736, 938
DpnI GATC 8 cut(s) 89, 150, 450, 714, 929, 996, 1040, 1158
DpnII GATC 8 cut(s) 87, 148, 448, 712, 927, 994, 1038, 1156
EaeI YGGCCR 1 cut(s) 918
Eam1104I CTCTTC 1 cut(s) 963
EarI CTCTTC 1 cut(s) 963
EciI GGCGGA 1 cut(s) 911
Eco130I CCWWGG 7 cut(s) 153, 193, 354, 477, 1047, 1062, 1099
Eco147I AGGCCT 1 cut(s) 1253
Eco47I GGWCC 2 cut(s) 898, 1230
Eco57I CTGAAG 4 cut(s) 165, 429, 1029, 1322
EcoNI CCTNNNNNAGG 1 cut(s) 1258
EcoO109I RGGNCCY 1 cut(s) 1230
EcoRII CCWGG 5 cut(s) 260, 524, 960, 1226, 1259
EcoT14I CCWWGG 7 cut(s) 153, 193, 354, 477, 1047, 1062, 1099
EcoT22I ATGCAT 1 cut(s) 1120
ErhI CCWWGG 7 cut(s) 153, 193, 354, 477, 1047, 1062, 1099
FaeI CATG 9 cut(s) 133, 202, 385, 566, 656, 680, 937, 1093, 1380
FalI AAGNNNNNCTT 2 cut(s) 974, 1006
FaqI GGGAC 4 cut(s) 102, 109, 943, 1158
FatI CATG 9 cut(s) 129, 198, 381, 562, 652, 676, 933, 1089, 1376
FbaI TGATCA 1 cut(s) 927
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 3 cut(s) 43, 515, 1217
FokI GGATG 1 cut(s) 608
Fsp4HI GCNGC 3 cut(s) 43, 515, 1217
FspAI RTGCGCAY 1 cut(s) 1088
FspBI CTAG 1 cut(s) 164
FspI TGCGCA 2 cut(s) 890, 1088
GlaI GCGC 4 cut(s) 363, 380, 890, 1088
GluI GCNGC 3 cut(s) 43, 515, 1217
GsaI CCCAGC 1 cut(s) 561
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 7 cut(s) 246, 581, 675, 920, 965, 1061, 1253
HapII CCGG 1 cut(s) 345
HgaI GACGC 1 cut(s) 350
HhaI GCGC 4 cut(s) 364, 381, 891, 1089
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 9 cut(s) 133, 202, 385, 566, 656, 680, 937, 1093, 1380
Hin6I GCGC 4 cut(s) 362, 379, 889, 1087
HinP1I GCGC 4 cut(s) 362, 379, 889, 1087
HincII GTYRAC 1 cut(s) 237
HindII GTYRAC 1 cut(s) 237
HinfI GANTC 2 cut(s) 208, 1146
HpaI GTTAAC 1 cut(s) 237
HpaII CCGG 1 cut(s) 345
HphI GGTGA 4 cut(s) 391, 898, 1099, 1184
Hpy166II GTNNAC 4 cut(s) 204, 237, 313, 1188
Hpy188I TCNGA 6 cut(s) 258, 301, 448, 730, 739, 1302
Hpy188III TCNNGA 7 cut(s) 172, 452, 593, 716, 800, 982, 1193
Hpy8I GTNNAC 4 cut(s) 204, 237, 313, 1188
HpyAV CCTTC 9 cut(s) 172, 625, 634, 919, 1279, 1297, 1312, 1333, 1354
HpyCH4III ACNGT 3 cut(s) 370, 422, 1130
HpyCH4V TGCA 6 cut(s) 338, 502, 514, 822, 1118, 1216
HpyF10VI GCNNNNNNNGC 5 cut(s) 583, 641, 672, 1058, 1067
HpyF3I CTNAG 4 cut(s) 61, 300, 736, 938
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 9 cut(s) 133, 202, 385, 566, 656, 680, 937, 1093, 1380
HspAI GCGC 4 cut(s) 362, 379, 889, 1087
Ksp22I TGATCA 1 cut(s) 927
KspAI GTTAAC 1 cut(s) 237
Kzo9I GATC 8 cut(s) 87, 148, 448, 712, 927, 994, 1038, 1156
LmnI GCTCC 2 cut(s) 144, 574
Lsp1109I GCAGC 3 cut(s) 54, 526, 1228
LweI GCATC 7 cut(s) 54, 217, 262, 262, 330, 489, 1165
MaeI CTAG 1 cut(s) 164
MaeIII GTNAC 5 cut(s) 384, 397, 904, 1015, 1105
MalI GATC 8 cut(s) 89, 150, 450, 714, 929, 996, 1040, 1158
MboI GATC 8 cut(s) 87, 148, 448, 712, 927, 994, 1038, 1156
MboII GAAGA 6 cut(s) 158, 580, 731, 775, 881, 980
MflI RGATCY 4 cut(s) 148, 448, 1038, 1156
MhlI GDGCHC 2 cut(s) 141, 429
MlsI TGGCCA 1 cut(s) 920
MluCI AATT 1 cut(s) 184
MluNI TGGCCA 1 cut(s) 920
MlyI GAGTC 2 cut(s) 217, 1140
MmeI TCCRAC 1 cut(s) 42
Mox20I TGGCCA 1 cut(s) 920
Mph1103I ATGCAT 1 cut(s) 1120
MscI TGGCCA 1 cut(s) 920
MseI TTAA 6 cut(s) 54, 236, 432, 1140, 1248, 1384
MslI CAYNNNNRTG 5 cut(s) 134, 218, 539, 1026, 1241
Msp20I TGGCCA 1 cut(s) 920
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 5 cut(s) 262, 526, 962, 1228, 1261
Mva1269I GAATGC 2 cut(s) 516, 1218
MvaI CCWGG 5 cut(s) 262, 526, 962, 1228, 1261
MwoI GCNNNNNNNGC 5 cut(s) 583, 641, 672, 1058, 1067
NdeII GATC 8 cut(s) 87, 148, 448, 712, 927, 994, 1038, 1156
NlaIII CATG 9 cut(s) 133, 202, 385, 566, 656, 680, 937, 1093, 1380
NlaIV GGNNCC 2 cut(s) 794, 900
NmuCI GTSAC 4 cut(s) 397, 904, 1015, 1105
NsbI TGCGCA 2 cut(s) 890, 1088
NsiI ATGCAT 1 cut(s) 1120
NspI RCATGY 6 cut(s) 202, 385, 656, 937, 1093, 1380
OliI CACNNNNGTG 2 cut(s) 539, 1026
PceI AGGCCT 1 cut(s) 1253
PciI ACATGT 2 cut(s) 652, 1376
PctI GAATGC 2 cut(s) 516, 1218
PflFI GACNNNGTC 1 cut(s) 902
PkrI GCNGC 3 cut(s) 44, 516, 1218
PleI GAGTC 2 cut(s) 216, 1140
PpsI GAGTC 2 cut(s) 216, 1140
PpuMI RGGWCCY 1 cut(s) 1230
PscI ACATGT 2 cut(s) 652, 1376
PsiI TTATAA 1 cut(s) 758
Psp5II RGGWCCY 1 cut(s) 1230
Psp6I CCWGG 5 cut(s) 260, 524, 960, 1226, 1259
PspFI CCCAGC 1 cut(s) 557
PspGI CCWGG 5 cut(s) 260, 524, 960, 1226, 1259
PspN4I GGNNCC 2 cut(s) 794, 900
PspPI GGNCC 2 cut(s) 898, 1230
PspPPI RGGWCCY 1 cut(s) 1230
PstNI CAGNNNCTG 1 cut(s) 592
PsuI RGATCY 4 cut(s) 148, 448, 1038, 1156
PsyI GACNNNGTC 1 cut(s) 902
RsaI GTAC 1 cut(s) 1187
RsaNI GTAC 1 cut(s) 1186
RseI CAYNNNNRTG 5 cut(s) 134, 218, 539, 1026, 1241
SaqAI TTAA 6 cut(s) 54, 236, 432, 1140, 1248, 1384
SatI GCNGC 3 cut(s) 43, 515, 1217
Sau3AI GATC 8 cut(s) 87, 148, 448, 712, 927, 994, 1038, 1156
Sau96I GGNCC 2 cut(s) 898, 1230
SchI GAGTC 2 cut(s) 217, 1140
ScrFI CCNGG 5 cut(s) 262, 526, 962, 1228, 1261
SduI GDGCHC 2 cut(s) 141, 429
SfaNI GCATC 7 cut(s) 54, 217, 262, 262, 330, 489, 1165
SinI GGWCC 2 cut(s) 898, 1230
SmiMI CAYNNNNRTG 5 cut(s) 134, 218, 539, 1026, 1241
SmlI CTYRAG 3 cut(s) 26, 248, 1073
SmoI CTYRAG 3 cut(s) 26, 248, 1073
Sse9I AATT 1 cut(s) 184
SseBI AGGCCT 1 cut(s) 1253
SsiI CCGC 1 cut(s) 896
SspMI CTAG 1 cut(s) 164
StuI AGGCCT 1 cut(s) 1253
StyD4I CCNGG 5 cut(s) 260, 524, 960, 1226, 1259
StyI CCWWGG 7 cut(s) 153, 193, 354, 477, 1047, 1062, 1099
TaaI ACNGT 3 cut(s) 370, 422, 1130
TaqI TCGA 2 cut(s) 282, 788
TaqII GACCGA 1 cut(s) 963
TasI AATT 1 cut(s) 184
TatI WGTACW 1 cut(s) 1185
Tru1I TTAA 6 cut(s) 54, 236, 432, 1140, 1248, 1384
Tru9I TTAA 6 cut(s) 54, 236, 432, 1140, 1248, 1384
TscAI CASTG 4 cut(s) 427, 541, 1030, 1135
TseFI GTSAC 4 cut(s) 397, 904, 1015, 1105
TseI GCWGC 3 cut(s) 42, 514, 1216
Tsp45I GTSAC 4 cut(s) 397, 904, 1015, 1105
TspDTI ATGAA 3 cut(s) 191, 653, 1325
TspRI CASTG 4 cut(s) 427, 541, 1030, 1135
Tth111I GACNNNGTC 1 cut(s) 902
VpaK11BI GGWCC 2 cut(s) 898, 1230
XagI CCTNNNNNAGG 1 cut(s) 1258
XceI RCATGY 6 cut(s) 202, 385, 656, 937, 1093, 1380
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 1 cut(s) 164
Zsp2I ATGCAT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.