MD12G1145300.v1.1

Thaumatin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005684
Physical Location & Seq
Reverse (-)
22440833 .. 22441904
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1145300.v1.1.491

Sequence Viewer

Length: 735 bp
ATGCCAACTTCCTCTTTTCTCTTCCCCGTACTCTATCTACTAATCTCTATCTCAATTACAAATGGGGCTCAACTGATTCTAGTGAACAACTGCCATGAAAGCATATGGCCCGGTATACTCGGCAGTGCAGGCGTAGACACTCCTCAAAGCGGCGGTTTACATCTTGGCAGCGGCGAGGAATCAGTTCTTGATGTACCTGAGAAGTGGTCAGGAAGAATATGGGGCAGGCAGGGTTGCTTCTTTGACAGTAATGGAAAAGGCAACTGTGAGACCGGCGATTGTTTTGGCCAATTGCATTGCCAAGGAAAAGGAGGCGTGCCTCCAGCGACCGTGGTTGAAATGACGCTCGGAACATCAACTTCACCCCTGCATTTCTATGATGTGAGTTTGGTTGATGGCTTTAACTTGCCCGTGTCAATGAAGCCGGTTGGTGGGGGAATAGGGTGCGGTGTTGCCTCATGCGAGGTTGATTTGAACGTGTGCTGCCCATCTGCGTTGGAAGTGAGGAGAGGAGGCAGGGTGGTCGGGTGTAAGAGTGCCTGCTTGGCTATGCAGTCTGCGAAGTATTGCTGCACAGGAAGCTACGCAAACCCAAACACTTGCAAACCAACCCTTTTCGCACATCTGTTTAAGGCCATATGCCCCAAGGCTTATAGTTATGCCTTTGATGACTCCTCCAGCCTTAACAAATGCCGGGCTTCGCGGTATGTCATCACTTTCTGCCCTCCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

245

Amino Acids

25.78

Weight (kDa)

6.94

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 29 - 242 1.5e-80 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 115, 135
AccII CGCG 1 cut(s) 703
AciI CCGC 5 cut(s) 150, 153, 171, 447, 703
AcoI YGGCCR 1 cut(s) 286
AfaI GTAC 2 cut(s) 30, 195
AfiI CCNNNNNNNGG 2 cut(s) 149, 431
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 2 cut(s) 338, 475
AluBI AGCT 1 cut(s) 582
AluI AGCT 1 cut(s) 582
Alw26I GTCTC 1 cut(s) 263
AoxI GGCC 3 cut(s) 107, 286, 633
ApeKI GCWGC 3 cut(s) 168, 483, 570
Asp700I GAANNNNTTC 1 cut(s) 183
AspS9I GGNCC 1 cut(s) 108
AsuC2I CCSGG 2 cut(s) 111, 695
AsuHPI GGTGA 1 cut(s) 354
BalI TGGCCA 1 cut(s) 288
BanII GRGCYC 1 cut(s) 70
BbvI GCAGC 3 cut(s) 180, 470, 557
BccI CCATC 2 cut(s) 389, 496
BcgI CGANNNNNNTGC 2 cut(s) 505, 539
BcnI CCSGG 2 cut(s) 111, 695
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 1 cut(s) 80
BglI GCCNNNNNGGC 1 cut(s) 545
BisI GCNGC 5 cut(s) 151, 169, 172, 484, 571
BlsI GCNGC 5 cut(s) 152, 170, 173, 485, 572
Bme1390I CCNGG 2 cut(s) 111, 695
BmgT120I GGNCC 1 cut(s) 108
BmrFI CCNGG 2 cut(s) 111, 695
BpmI CTGGAG 2 cut(s) 306, 661
BpuMI CCSGG 2 cut(s) 111, 695
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 3 cut(s) 301, 330, 645
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 431
Bse118I RCCGGY 2 cut(s) 272, 424
Bse3DI GCAATG 1 cut(s) 295
BseDI CCNNGG 3 cut(s) 301, 330, 645
BseLI CCNNNNNNNGG 2 cut(s) 149, 431
BseMI GCAATG 1 cut(s) 295
BseMII CTCAG 1 cut(s) 189
BseRI GAGGAG 4 cut(s) 132, 520, 525, 664
BseXI GCAGC 3 cut(s) 180, 470, 557
BsgI GTGCAG 2 cut(s) 147, 556
Bsh1236I CGCG 1 cut(s) 703
Bsh1285I CGRYCG 1 cut(s) 330
BshFI GGCC 3 cut(s) 109, 288, 635
BsiEI CGRYCG 1 cut(s) 330
BsiSI CCGG 4 cut(s) 111, 273, 425, 694
BslI CCNNNNNNNGG 2 cut(s) 149, 431
BsmAI GTCTC 1 cut(s) 263
BsnI GGCC 3 cut(s) 109, 288, 635
Bso31I GGTCTC 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 70
BspACI CCGC 5 cut(s) 150, 153, 171, 447, 703
BspANI GGCC 3 cut(s) 109, 288, 635
BspCNI CTCAG 1 cut(s) 190
BspFNI CGCG 1 cut(s) 703
BspTNI GGTCTC 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 295
BsrFI RCCGGY 2 cut(s) 272, 424
BssAI RCCGGY 2 cut(s) 272, 424
BssECI CCNNGG 3 cut(s) 301, 330, 645
BssNAI GTATAC 1 cut(s) 116
BssT1I CCWWGG 2 cut(s) 301, 645
Bst1107I GTATAC 1 cut(s) 116
Bst4CI ACNGT 3 cut(s) 248, 266, 331
Bst6I CTCTTC 1 cut(s) 26
BstC8I GCNNGC 4 cut(s) 130, 227, 317, 541
BstDEI CTNAG 1 cut(s) 198
BstDSI CCRYGG 1 cut(s) 330
BstFNI CGCG 1 cut(s) 703
BstMAI GTCTC 1 cut(s) 263
BstMCI CGRYCG 1 cut(s) 330
BstMWI GCNNNNNNNGC 4 cut(s) 99, 129, 545, 579
BstSCI CCNGG 2 cut(s) 109, 693
BstUI CGCG 1 cut(s) 703
BstV1I GCAGC 3 cut(s) 180, 470, 557
BstZ17I GTATAC 1 cut(s) 116
BsuRI GGCC 3 cut(s) 109, 288, 635
BtgI CCRYGG 1 cut(s) 330
BtsI GCAGTG 1 cut(s) 130
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 4 cut(s) 130, 227, 317, 541
Cfr10I RCCGGY 2 cut(s) 272, 424
Cfr13I GGNCC 1 cut(s) 108
CseI GACGC 1 cut(s) 352
Csp6I GTAC 2 cut(s) 29, 194
CviAII CATG 2 cut(s) 95, 459
CviQI GTAC 2 cut(s) 29, 194
DdeI CTNAG 1 cut(s) 198
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 301, 645
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 263
EcoT14I CCWWGG 2 cut(s) 301, 645
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 2 cut(s) 301, 645
FaeI CATG 2 cut(s) 98, 462
FatI CATG 2 cut(s) 94, 458
FauNDI CATATG 2 cut(s) 104, 638
FblI GTMKAC 2 cut(s) 115, 135
Fnu4HI GCNGC 5 cut(s) 151, 169, 172, 484, 571
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 5 cut(s) 151, 169, 172, 484, 571
FspBI CTAG 1 cut(s) 80
GluI GCNGC 5 cut(s) 151, 169, 172, 484, 571
GsuI CTGGAG 2 cut(s) 306, 661
HaeIII GGCC 3 cut(s) 109, 288, 635
HapII CCGG 4 cut(s) 111, 273, 425, 694
HgaI GACGC 1 cut(s) 352
Hin1II CATG 2 cut(s) 98, 462
HinfI GANTC 3 cut(s) 76, 179, 671
HpaII CCGG 4 cut(s) 111, 273, 425, 694
HphI GGTGA 1 cut(s) 354
Hpy166II GTNNAC 4 cut(s) 85, 116, 136, 158
Hpy188I TCNGA 1 cut(s) 350
Hpy188III TCNNGA 2 cut(s) 188, 210
Hpy8I GTNNAC 4 cut(s) 85, 116, 136, 158
HpyCH4III ACNGT 3 cut(s) 248, 266, 331
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 6 cut(s) 128, 295, 370, 553, 573, 603
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 129, 545, 579
HpyF3I CTNAG 1 cut(s) 198
HpySE526I ACGT 1 cut(s) 477
Hsp92II CATG 2 cut(s) 98, 462
Lsp1109I GCAGC 3 cut(s) 180, 470, 557
MaeI CTAG 1 cut(s) 80
MaeII ACGT 1 cut(s) 477
MboII GAAGA 2 cut(s) 13, 225
MfeI CAATTG 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 70
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 2 cut(s) 54, 290
MluNI TGGCCA 1 cut(s) 288
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 1 cut(s) 477
Mox20I TGGCCA 1 cut(s) 288
MroXI GAANNNNTTC 1 cut(s) 183
MscI TGGCCA 1 cut(s) 288
MseI TTAA 3 cut(s) 402, 630, 684
MslI CAYNNNNRTG 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 288
MspA1I CMGCKG 1 cut(s) 171
MspI CCGG 4 cut(s) 111, 273, 425, 694
MspR9I CCNGG 2 cut(s) 111, 695
MunI CAATTG 1 cut(s) 290
MvnI CGCG 1 cut(s) 703
MwoI GCNNNNNNNGC 4 cut(s) 99, 129, 545, 579
NciI CCSGG 2 cut(s) 111, 695
NdeI CATATG 2 cut(s) 104, 638
NlaIII CATG 2 cut(s) 98, 462
NmeAIII GCCGAG 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 2 cut(s) 76, 179
PkrI GCNGC 5 cut(s) 152, 170, 173, 485, 572
PleI GAGTC 1 cut(s) 665
PpsI GAGTC 1 cut(s) 665
PspPI GGNCC 1 cut(s) 108
RsaI GTAC 2 cut(s) 30, 195
RsaNI GTAC 2 cut(s) 29, 194
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 3 cut(s) 402, 630, 684
SatI GCNGC 5 cut(s) 151, 169, 172, 484, 571
Sau96I GGNCC 1 cut(s) 108
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 2 cut(s) 111, 695
SduI GDGCHC 1 cut(s) 70
SetI ASST 4 cut(s) 199, 468, 480, 584
SmiMI CAYNNNNRTG 1 cut(s) 375
Sse9I AATT 2 cut(s) 54, 290
SsiI CCGC 5 cut(s) 150, 153, 171, 447, 703
SspMI CTAG 1 cut(s) 80
StyD4I CCNGG 2 cut(s) 109, 693
StyI CCWWGG 2 cut(s) 301, 645
TaaI ACNGT 3 cut(s) 248, 266, 331
TaiI ACGT 1 cut(s) 480
TasI AATT 2 cut(s) 54, 290
TauI GCSGC 2 cut(s) 153, 174
TfiI GAWTC 2 cut(s) 76, 179
Tru1I TTAA 3 cut(s) 402, 630, 684
Tru9I TTAA 3 cut(s) 402, 630, 684
TscAI CASTG 1 cut(s) 130
TseI GCWGC 3 cut(s) 168, 483, 570
TspDTI ATGAA 2 cut(s) 111, 434
TspRI CASTG 1 cut(s) 130
XmiI GTMKAC 2 cut(s) 115, 135
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.