Rroxscaffold_6G00414800

Thaumatin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005684
Physical Location & Seq
Reverse (-)
37084514 .. 37085534
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414800.1

Sequence Viewer

Length: 735 bp
ATGCCTACTTCCTCTCTTCTCTTTCCCTTGTTATATATATTAGCCTCAATCTCAATTACAAATGGGGCTCAACTCATTCTAGTAAACAACTGCAATGAAAGCATATGGCCTGGTATACTTGGAGGTGCAGGCCTAGCCACCCCCCAAAGCGGCGGTTTCCATCTTGGAAGTGGTGAGGAAGTGGTTCTTGATGTGCCTGAAAAATGGTCAGGAAGGATATGGGGAAGGCAGGGTTGCTCCTTTGACAGTAATGGAAAGGGTAACTGTGAAACTGGTGACTGTTTTGGCCAATTGCATTGCCAAGGAAAAGGCGGTATTCCCCCAGCAACTGTGGTCGAAATGACACTTGGATCATCAAATTCACCCCTCCATTTCTATGACGTGAGCTTGGTCGATGGCTTCAATTTGCCAGTGTCCATGAAACCTGTTGGGGGTGGAATAGGTTGCGGTGTTGCTTCATGTGAAGTTGACTTGAACATTTGCTGCCCATCAGCTTTGGAAGTGAGGAGAGGAGGCAAGGTCGTGGGGTGTAAAAGTGCCTGCTTGGCTATGCAATCTGCCAAGTATTGCTGCACTGGAAACTATGCAAACCCAAACACTTGCAAGCCAACTCTTTTTGCTCATCTATTTAAGGCTATATGCCCCAAGGCTTATAGCTATGCTTTTGATGACTCTTCAAGCCTTAACAGATGCAGGGCTTCGCGGTATGTCATCACTTTCTGCCCTCCCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

244

Amino Acids

25.66

Weight (kDa)

7.39

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 29 - 242 7.1e-82 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 115
AccII CGCG 1 cut(s) 703
AciI CCGC 5 cut(s) 150, 153, 312, 447, 703
AclWI GGATC 1 cut(s) 358
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 358
AfiI CCNNNNNNNGG 2 cut(s) 149, 431
AgsI TTSAA 3 cut(s) 403, 475, 678
AjiI CACGTC 1 cut(s) 382
AjnI CCWGG 1 cut(s) 109
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AluBI AGCT 3 cut(s) 387, 494, 657
AluI AGCT 3 cut(s) 387, 494, 657
AlwI GGATC 1 cut(s) 358
AlwNI CAGNNNCTG 1 cut(s) 329
AoxI GGCC 3 cut(s) 107, 130, 286
ApeKI GCWGC 2 cut(s) 483, 570
ApoI RAATTY 1 cut(s) 358
Asp700I GAANNNNTTC 1 cut(s) 183
AsuHPI GGTGA 3 cut(s) 185, 287, 354
BalI TGGCCA 1 cut(s) 288
BanII GRGCYC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 470, 557
BccI CCATC 3 cut(s) 168, 389, 496
BciT130I CCWGG 1 cut(s) 111
BfaI CTAG 2 cut(s) 80, 134
BglI GCCNNNNNGGC 1 cut(s) 545
BisI GCNGC 3 cut(s) 151, 484, 571
BlsI GCNGC 3 cut(s) 152, 485, 572
Bme1390I CCNGG 1 cut(s) 111
BmgBI CACGTC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 680
BsaJI CCNNGG 2 cut(s) 301, 645
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 431
Bse1I ACTGG 3 cut(s) 277, 410, 580
Bse3DI GCAATG 2 cut(s) 100, 295
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 301, 645
BseLI CCNNNNNNNGG 2 cut(s) 149, 431
BseMI GCAATG 2 cut(s) 100, 295
BseNI ACTGG 3 cut(s) 277, 410, 580
BseRI GAGGAG 2 cut(s) 520, 525
BseXI GCAGC 2 cut(s) 470, 557
BseYI CCCAGC 1 cut(s) 322
BsgI GTGCAG 2 cut(s) 147, 556
Bsh1236I CGCG 1 cut(s) 703
BshFI GGCC 3 cut(s) 109, 132, 288
BslI CCNNNNNNNGG 2 cut(s) 149, 431
BsnI GGCC 3 cut(s) 109, 132, 288
Bsp1286I GDGCHC 1 cut(s) 70
Bsp143I GATC 1 cut(s) 350
BspACI CCGC 5 cut(s) 150, 153, 312, 447, 703
BspANI GGCC 3 cut(s) 109, 132, 288
BspFNI CGCG 1 cut(s) 703
BspPI GGATC 1 cut(s) 358
BsrDI GCAATG 2 cut(s) 100, 295
BsrI ACTGG 3 cut(s) 277, 410, 580
BssECI CCNNGG 2 cut(s) 301, 645
BssMI GATC 1 cut(s) 350
BssNAI GTATAC 1 cut(s) 116
BssT1I CCWWGG 2 cut(s) 301, 645
Bst1107I GTATAC 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 4 cut(s) 248, 266, 281, 331
Bst6I CTCTTC 2 cut(s) 21, 679
BstC8I GCNNGC 3 cut(s) 130, 541, 605
BstFNI CGCG 1 cut(s) 703
BstKTI GATC 1 cut(s) 353
BstMBI GATC 1 cut(s) 350
BstMWI GCNNNNNNNGC 3 cut(s) 99, 134, 545
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 109
BstUI CGCG 1 cut(s) 703
BstV1I GCAGC 2 cut(s) 470, 557
BstZ17I GTATAC 1 cut(s) 116
BsuRI GGCC 3 cut(s) 109, 132, 288
BtrI CACGTC 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 417, 573
Cac8I GCNNGC 3 cut(s) 130, 541, 605
CaiI CAGNNNCTG 1 cut(s) 329
CviAII CATG 2 cut(s) 418, 459
DpnI GATC 1 cut(s) 352
DpnII GATC 1 cut(s) 350
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 21, 679
EarI CTCTTC 2 cut(s) 21, 679
Eco130I CCWWGG 2 cut(s) 301, 645
Eco147I AGGCCT 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 301, 645
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 2 cut(s) 301, 645
FaeI CATG 2 cut(s) 421, 462
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FatI CATG 2 cut(s) 417, 458
FauNDI CATATG 1 cut(s) 104
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 151, 484, 571
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 3 cut(s) 151, 484, 571
FspBI CTAG 2 cut(s) 80, 134
GluI GCNGC 3 cut(s) 151, 484, 571
GsaI CCCAGC 1 cut(s) 326
HaeIII GGCC 3 cut(s) 109, 132, 288
Hin1II CATG 2 cut(s) 421, 462
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HinfI GANTC 1 cut(s) 671
HphI GGTGA 3 cut(s) 185, 287, 354
Hpy166II GTNNAC 3 cut(s) 85, 116, 469
Hpy188III TCNNGA 2 cut(s) 188, 210
Hpy8I GTNNAC 3 cut(s) 85, 116, 469
HpyAV CCTTC 2 cut(s) 207, 219
HpyCH4III ACNGT 4 cut(s) 248, 266, 281, 331
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 8 cut(s) 93, 128, 295, 553, 573, 587, 603, 693
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 134, 545
HpySE526I ACGT 1 cut(s) 381
Hsp92II CATG 2 cut(s) 421, 462
Kzo9I GATC 1 cut(s) 350
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 2 cut(s) 470, 557
LweI GCATC 1 cut(s) 680
MaeI CTAG 2 cut(s) 80, 134
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 2 cut(s) 260, 275
MalI GATC 1 cut(s) 352
MboI GATC 1 cut(s) 350
MboII GAAGA 2 cut(s) 8, 666
MfeI CAATTG 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 70
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 4 cut(s) 54, 290, 358, 403
MluNI TGGCCA 1 cut(s) 288
MlyI GAGTC 1 cut(s) 665
MnlI CCTC 9 cut(s) 22, 55, 116, 169, 377, 498, 503, 506, 735
Mox20I TGGCCA 1 cut(s) 288
MroXI GAANNNNTTC 1 cut(s) 183
MscI TGGCCA 1 cut(s) 288
MseI TTAA 2 cut(s) 630, 684
MslI CAYNNNNRTG 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 288
MspR9I CCNGG 1 cut(s) 111
MunI CAATTG 1 cut(s) 290
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 703
MwoI GCNNNNNNNGC 3 cut(s) 99, 134, 545
NdeI CATATG 1 cut(s) 104
NdeII GATC 1 cut(s) 350
NlaIII CATG 2 cut(s) 421, 462
NmuCI GTSAC 1 cut(s) 275
PceI AGGCCT 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 183
PkrI GCNGC 3 cut(s) 152, 485, 572
PleI GAGTC 1 cut(s) 665
PpsI GAGTC 1 cut(s) 665
Psp6I CCWGG 1 cut(s) 109
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 329
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 2 cut(s) 630, 684
SatI GCNGC 3 cut(s) 151, 484, 571
Sau3AI GATC 1 cut(s) 350
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 1 cut(s) 70
SetI ASST 8 cut(s) 127, 384, 389, 427, 445, 496, 522, 659
SfaNI GCATC 1 cut(s) 680
SmiMI CAYNNNNRTG 1 cut(s) 375
Sse9I AATT 4 cut(s) 54, 290, 358, 403
SseBI AGGCCT 1 cut(s) 132
SsiI CCGC 5 cut(s) 150, 153, 312, 447, 703
SspMI CTAG 2 cut(s) 80, 134
StuI AGGCCT 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 2 cut(s) 301, 645
TaaI ACNGT 4 cut(s) 248, 266, 281, 331
TaiI ACGT 1 cut(s) 384
TaqI TCGA 2 cut(s) 336, 393
TasI AATT 4 cut(s) 54, 290, 358, 403
TauI GCSGC 1 cut(s) 153
Tru1I TTAA 2 cut(s) 630, 684
Tru9I TTAA 2 cut(s) 630, 684
TscAI CASTG 2 cut(s) 417, 580
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 2 cut(s) 483, 570
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 3 cut(s) 111, 434, 447
TspRI CASTG 2 cut(s) 417, 580
XapI RAATTY 1 cut(s) 358
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 2 cut(s) 80, 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.