Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004063

Physical Location & Seq

Genomic Coordinates Reverse (-)

29544821 .. 29556246

Gene Structure 11426 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1217900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5724 bp

ATGGCGGCTTCGGAAGCTTTCAGCGTACGGATCGTCTCCGTCGACTACTACATGGCGCCGCCGATCCCAGGCCTCGATATCTCTTACAGTAGCTTCCAAGGTGGGAAGGTGAATGAGGTGCCAGTTATAAGAATATTCGGCGCCACGCCGGCTGGTCAGAAGACTTGCTTGCACATTCATAGAGCATTACCATACTTATATGTGCCATGCGCTGATATCCCGATTCAGCTGCACCAGAAAGGTGATTCATACACACATGATGTAGCTCTTGCTATAGAGAAAGCTTTGAAGCTCAAAGGTAATGCTGGTTCAAAGCGACAGCATGTACATGGTTGCAGCCTTGTCCAGGCTAGGAAGCTTTATGGTTACCACTCATCGGAGGAGTTGTTCATTTCTTCCCCATTCATTGTGGGAAGTTCAGTTTTTGATAAATGCTTACAGCCTCATGAATCTCACATTCCCTTCATTCTTCAATTTATGGTTGACTACAACTTGTATGGGATGGGTCATCTACATGTATCAAAGATTAAATTCCGTCATCCAATACCAGATGTTTTCGTCCATAGAAGGTCTGTTTACAATGGACAGCCTAGTCAGGACATAGATAAAACGACTTGCATGTCTGCTGATTTTCAGGCAGATATACGTGGCCATCTGTCTTTGGGTTCACCTGTTTGGATATCTTCTACAATTCCAGTTGATTGGACATGGAATTCTCCTGGTGAATTTGAAGGTTCCTCAGATACAGATATTAACAGCATTAAACGTCAAAGTATTTGTGAACTTGAGGGCGATTCTACTGTAGATGAGGTTCTTAATCAGCAGCTTAAGATGTATACTTCCCTCTCACAAACTTGTTCAGATGTGAAGATGGTTCAGTCACTTGTACCAATTTTGGAGGAGTGTGAAAGGACTGGAATACATGAGGCAGCAATACCACCTGATCCTGCCAAACCACTTGCTGAAGAAGCTTTGAAAACTCTCTCGGTTGGTCTTGAATTTGACAACAAGCTTATTAAAGTGCACAGTGAAGCTGAAAGTTCTCTGTGTCGAACTCAATTAAGGAAAGATGTAAGGTCTGTGGAGCCGATGGCATCTCAAAAAGATGAAGGAAACTTTGCTGGACCCCAAACTGATAATCTTATTGATGGAGAAATAATTGGATCTCTCCCAACACAAGATTCTACTAAAGATAGTGTTCCAAATGCCAAACTATCATTGAATAAGGAACTTCCATCATCACAAGTAATCCCGACGGTAAACATGAAGGTTGCAGATAGTGAAGCACTAGGTCTTCTTAGATGGCTTGCCACTTCTCAGGCCACTGATGATATAAACTCTGATGACGAACTTGTTTGTGAAACAATTCTTAGTCCCTTGTTGCCGGCAACAACAATTGACAATGTTCTAAACAAAGCTAATGTGGATTATGAGAATGAATCCCAAAAGGAGTGTCAGGACATACTTGATTCGGTTGGTGATATGATTGACTTTGAAAGATTGCAAGAAAGAGCTTCTTACCCTTCAGATCGAAAGAATTCTTCAAAAAGTTCGTCTGAGTATGTGATTCCCCAGGTGGATGGTTGTGGGGATGACGATTTCTCAACTCCTTGTAATGGATCAGTTGGAAGCTTTTCTGAAATAGAGGGGATGAGTGAATTTAGGACCTCCAGCCATCAGGTGCAAGATGCTAGCTCCATCTTCAATCATAAGCACAAAAGGAAAAAATTATGGGGCTCTTTACCTTTCTCTGAGACCTCAAAGATGAAGACAGAAGGGGAACATGCCAGTTTACACATTGAAACAAAGGACCCTAGTGGTACAAGTTCTTCTGAAAATGAAGTGGGAAAACGTGCAGGCGCTGGAACTTGTGATGCAAAAGACAGTAGTATGTTGGCAAGATGCTCTGTGAGGGACTTGATGAGGAGAAAGCGATCCTACCGAGTTGAGCCACCTGAAAGTAGTGGGAGGGAGGAAAACGAAGACACACTTCTCTGCGCAAAGCATTTAGATTTTCAAGTATCAGGTGCTGATGAGCTTGACAGGAAGCCTCACGAGTCTTCTAACTTCAGGCTATCATTAGCTGACCAACAATCAGGAAGTTATAAGGCTTATATATATGATGCTAAACCAACCAATCCTGGTTGTTCTCTGTATGGTAAACTTCCACTGTGTTCTAGCAATGACAACATTTTACATGCAAGTACGTTAGATGGAAAATTGGATTCCAATGTGTTGATTGGTGAAGAAGTTGGTGCTGGTGCAATGGCTCAATTTCGGAACTTCTTGATTACAGGCCCTTCTCAAGTTCCTTCTGTGAACAAAAGAATGCATACTTGGTCTGCTGAGCATGAAGTGGCTGATGGAGATGGTTATGTTGAAAGTGGCTTTGAAAGCTTATCTTCCAACGGTGGAGTATATGAAATGTCTTTTGAGAATCCCATGGGTGCAAATGCCACAACTGTCAGTGCTGTTCTAAACAATGAGAGGTCTGGTGTCCAAAAACTGGGTAGGGACTCTGTTTCATCTGGGCTCAGACACTCACCGTCTGAGGTCATCTATCCGGATGATGAGGACTTAATTGGAATGACATTCCACAAGAAGCCTCCTGTTCCAGACTGGAAGGACAGGGCATCTAAAGACGCTTCATCTTTATACGATGGAAGAGCCACGGATGACTATTTCCCCTTCTTTGTGGGAGATTTCCCGGACGAGAAAGAAATTCAGAACAAGTGTGTTAGAAGTGAATCTAGCTCTCATGAAGAATCTGTTATGGGTGTCCCTACTCACTATCAAACTGATGGCTCTTTCTTGTATTTGTTAACACCTGCAACTTCACCCCCTTCTGCTGAAAAGGTGAATAGATGGTTGTCAAGTGATGAAAAAGATGATGTTCCACCTATTCTCCCCCAAGGATCTCAAGAGAATCATGAAAATCATGAAGAAGAAAGTAAACATTTCTGCACTGACAGGACGGGAATAGCACAAAGAGAAGGAGATGCTGTTAAAGTTCAAACAAACTCTGAATGCTCACAAGATAATTCTCAGATTTCTGGCCCAGATGGGAGATCAAAGCTCACTCCTCTTAGTCAAATTGGATTCAGGGACCCTGCAAGTGTTGGCGCAGGCCAACAGCTGACATTGTTAAGCATAGAGGTTCAAGCAGAATCAAGAGGAGATCTTCGACCTGATCCTCGGTTTGATGCCATCAATTTTATCTCTCTTGCTATTCAGAATGATAGTGATCCTATTGTTGAAGTTCATGTGCTTTTGCACAGTAAAGCTGAAGGTTCTCAGAGGAGTCTTGATGGAATTTCTGGCTGCAAGGTGTTGGTTTTTTATGAGGAGAAGTACTTATTTGATCATTTTATGAAAATTGTGTGCCCATTGGACCCAGATATTTTGATGGGTTGGGATATACAAAGTGGTTCTCTTGGCTTTTTGGCAGAAAGGGCTTCATATCTTGGTATAGGTTTACTCAACAAAATATCTCGGGCACCATCTGAATCCAAGGTGGAGGGTGCAGATTTAGAAGTTTTGGAGAAAGCAATACAAGATAAAATAATTCCTGAGGCAGTCATTGCCAATTCAGTTGTAAGAGAAGATCCAGTAATTGAGGATGAATGGGGAAGAACTCATGCCAGCGGTGTCCATGTTTGTGGTAGAATTATCCTTAATATATGGCGACTGATCCGTGGTGAAGTCAAGCTCAATATGTACACAGTTGAGGCTGTTGCTCAAGCTGTGTTGAGGCGAAAAGTCCCATATATTTCTAATAAAGTACTGGCAAAATGGTTTTCAAGTGGCCCTGGACAAGCCAGATATCGATGTATTGAATATGTAAATGAGAGAGCGAAGCTGAGCCTTGAAATAATGAATCAGTTGGACATGATAAATCGAACATCAGAACTTGCACGTGTCTTTGGCATTGACTTTTTTTCAGTTCTTTCTAGAGGTTCACAATACCGTGTCGAATCCATGTTTCTGAGATTAGCCCATGCACAAAACTATGTTGCCATATCTCCTGGGAGTAAACAGGTTGCTTCTCAACCAGCAATGGAGTGCATACCTCTTGTCATGGAACCAGAGTCTGGTTTTTATGCAGATCCAGTTGTCGTGTTGGATTTTCAGTCTCTTTATCCATCAATGATTATTGCATACAATCTTTGCTATTCTACATGCCTTGGAAAAGTTGTACCTTCAGAGGCAAATACACTTGGAGTTAGTTCGTTTTCACCAGATCCCCATGACCTTCATATTTTAAAAGATCAGATTCTGCTTACTCCAAATGGTGTCATGTACGTACCCGAGAAGGTTCGCAAAGGTGTATTACCCCGTTTGTTAGATGAAATATTGTCAACAAGAATAATGGTTAAACAAGCTATGAAAAAGCTGTCTTCTTCACAGCAAGTTCTTCACAGGATTTTTAATGCAAGACAGCTTGCTTTGAAGTTGATATCAAACGTAACGTATGGCTATACTGCTGCTGGGTTTAGTGGCCGCATGCCTTGTGCCGAGATTGCAGACAGTATTGTTCAATGTGGCCGTAGTACACTGGAAAAGGCTATTTCATACGTAAATGCACATGACAAGTGGAAGGCAAAAGTTATTTATGGCGACACAGACAGCATGTTTGTCCTCCTAAAAGGACGCACTCTTGAAGATTCTTTTAAGATTGGCCGCGAGATTGCATCTGAAATTAGTGCTATGAATCCGCATCCAGTTGCTTTGAAGATGGAAAAAGTTTACAGCCCGTGCTTTCTCCTTACTAAGAAGCGTTATGTTGGTTACAGTTATGAAAGCCCTGAACAGATCAAACCTATTTTTGATGCTAAAGGTATTGAGACAGTTCGTAGGGATACATGTGCGGCTGTTGCCAAGACAATGGAACAGTCTTTGAGACTTTTCTTTGAACATCAGGACATGTATGAGGTCAAAACATACTTACAGCGTCAGTGGAAGCGAATCATATCAGGAAGGGTGTCTCTTCAGGATTTTGTATTTGCAAAGGAAGTTCGCCTAGGCACCTATCGGGCAAGTTCTATTTCGTCACTTCCACCAGCTGCAGTTGTTGCCACAAAAGCTATGAAAACCGACCCTAGGGCAGAACCACGTTATGCAGAGCGAATACCTTACGTTGTGATCCATGGGGAGCCTGGGGCTCGTCTGGTTGACATGGTCGTGGATCCATTGAATCTCTTGGCTATTGATTCACCGTACAGATTAAATGATCTTTATTACATCCATAAACAGATAATCCCAGCTTTGCAGCGAGTGTTTGGGCTTCTTGGGGCTGACTTGAACGAGTGGTTTTCAGATATGCCCCGGCCAGCCAGGGAAGCCATTGGTAAACGCGCCTTTTATGCTTCCAATCCACACCGAACCAGAATTGATTATTACTATCTTTCAAGGCATTGTATCTTGTGCGGGGAGTTGGTCCAGGGATCAGCCCATCTATGCAATCAATGTTCTGAAAACAAAAGTTGTGCTGCTGTAGCTGTAATTGGAAGAACTTCGAAACTGGAGAGAGAGATGCAGCAGCTTGCTGCTATCTGTCGACATTGTGGAGGAGGGGACTGGGTCATAGACAGTGGCATTAAATGCACTTCTCTTGGATGCTCGGTGTTCTACGAGAGGCGTAAGGTTCAGAAAGAATTACAAGGGCTCGCTTCTGTTGCTGCAGAAACCGGTTTCTACCCGAAATGCATGGTGGAGTGGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000731 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010225 GO:0016035 GO:0018130 GO:0019438 GO:0019985 GO:0032991 GO:0033554 GO:0034641 GO:0034644 GO:0034645 GO:0034654 GO:0042276 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0071214 GO:0071478 GO:0071482 GO:0071494 GO:0071704 GO:0071897 GO:0090304 GO:0104004 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko01100 ko01524 ko03460 map01100 map01524 map03460

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 RVT_2 zf-C4pol zf-RanBP

Protein Analysis

1908

Amino Acids

211.5

Weight (kDa)

5.7

Isoelectric Point (pI)

47.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
REV3_N	PF24065	7 - 60	6.1e-19	DNA polymerase zeta catalytic subunit, N-terminal
POL3_N	PF24055	62 - 131	8.9e-16	DNA polymerase delta catalytic subunit-like, N-terminal domain
DNA_pol_B_exo1	PF03104	1044 - 1171	7.8e-10	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	1312 - 1760	3.3e-101	DNA polymerase family B
zf-C4pol	PF14260	1806 - 1879	3.9e-13	C4-type zinc-finger of DNA polymerase delta

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004063)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500
fragaria_vesca	FvH4_6g06120 FvH4_6g06120 FvH4_6g06120 FvH4_6g06120 FvH4_6g06120
malus_domestica	MD05G1053400.v1.1 MD05G1053600.v1.1 MD05G1053700.v1.1 MD12G1217900.v1.1
prunus_persica	Prupe.6G324000_v2.0.a1 Prupe.6G324000_v2.0.a1 Prupe.6G324000_v2.0.a1
pyrus_communis	pycom05g04420 pycom12g20340
rosa_chinensis	RchiOBHm_Chr3g0455131
rosa_laevigata	RLG00000025385
rosa_multiflora	Rmu_co8200746.1_g000001 Rmu_sc0041669.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424540
rosa_rugosa	Rorug01G0381700 Rorug03G0004000
rosa_samantha	Rh3AG064900 Rh3BG066900 Rh3CG065300 Rh3DG066800
rosa_wichuraiana	Rw3G005080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	128, 2106
AarI	CACCTGC	1 cut(s)	2836
AasI	GACNNNNNNGTC	2 cut(s)	591, 619
Acc16I	TGCGCA	1 cut(s)	1999
Acc36I	ACCTGC	1 cut(s)	2836
AccB1I	GGYRCC	5 cut(s)	55, 118, 140, 3496, 5024
AccB7I	CCANNNNNTGG	2 cut(s)	2506, 4091
AccI	GTMKAC	3 cut(s)	42, 836, 5557
AccII	CGCG	2 cut(s)	4683, 5355
AccIII	TCCGGA	1 cut(s)	2563
AciI	CCGC	8 cut(s)	5, 59, 3645, 4501, 4681, 4715, 4868, 5427
AcoI	YGGCCR	5 cut(s)	649, 4498, 4543, 4678, 5327
AcsI	RAATTY	8 cut(s)	530, 712, 725, 998, 1537, 1658, 2721, 3312
AcuI	CTGAAG	6 cut(s)	984, 1509, 2053, 3306, 4185, 4973
AcvI	CACGTG	1 cut(s)	3917
AcyI	GRCGYC	2 cut(s)	56, 141
AfiI	CCNNNNNNNGG	7 cut(s)	68, 346, 376, 1616, 2506, 4091, 5100
AflII	CTTAAG	1 cut(s)	827
AflIII	ACRYGT	4 cut(s)	514, 3916, 4862, 4923
AgeI	ACCGGT	1 cut(s)	5687
AjuI	GAANNNNNNNTTGG	4 cut(s)	1165, 1197, 2320, 2352
AloI	GAACNNNNNNTCC	2 cut(s)	371, 403
Alw21I	GWGCWC	1 cut(s)	1026
Alw26I	GTCTC	6 cut(s)	40, 1748, 4137, 4838, 4894, 4989
Alw44I	GTGCAC	1 cut(s)	1022
AlwNI	CAGNNNCTG	4 cut(s)	1862, 2030, 4091, 4276
Ama87I	CYCGRG	2 cut(s)	3492, 4307
Aor13HI	TCCGGA	1 cut(s)	2563
ApaLI	GTGCAC	1 cut(s)	1022
ApoI	RAATTY	8 cut(s)	530, 712, 725, 998, 1537, 1658, 2721, 3312
ArsI	GACNNNNNNTTYG	6 cut(s)	1438, 1470, 1538, 1570, 4616, 4648
AsiGI	ACCGGT	1 cut(s)	5687
Asp700I	GAANNNNTTC	4 cut(s)	1365, 1537, 1633, 5512
AspA2I	CCTAGG	2 cut(s)	5020, 5099
AspLEI	GCGC	7 cut(s)	58, 143, 212, 1862, 2000, 3125, 5357
AspS9I	GGNCC	9 cut(s)	1124, 1665, 1810, 2297, 3056, 3106, 3391, 3806, 5437
AsuC2I	CCSGG	2 cut(s)	2708, 5326
AsuII	TTCGAA	1 cut(s)	5516
AsuNHI	GCTAGC	1 cut(s)	1691
AvaI	CYCGRG	2 cut(s)	3492, 4307
AvaII	GGWCC	6 cut(s)	1124, 1665, 1810, 3106, 3391, 5437
AvrII	CCTAGG	2 cut(s)	5020, 5099
AxyI	CCTNAGG	1 cut(s)	3570
BaeGI	GKGCMC	3 cut(s)	1026, 3386, 3499
BalI	TGGCCA	1 cut(s)	651
BamHI	GGATCC	1 cut(s)	5185
BanI	GGYRCC	5 cut(s)	55, 118, 140, 3496, 5024
BanII	GRGCYC	4 cut(s)	1739, 2535, 5164, 5667
BarI	GAAGNNNNNNTAC	2 cut(s)	1813, 1845
BauI	CACGAG	1 cut(s)	2054
BbrPI	CACGTG	1 cut(s)	3917
BbsI	GAAGAC	6 cut(s)	167, 1286, 1775, 1989, 2052, 4389
Bbv12I	GWGCWC	1 cut(s)	1026
BceAI	ACGGC	1 cut(s)	4530
BcgI	CGANNNNNNTGC	2 cut(s)	211, 245
BciVI	GTATCC	1 cut(s)	4852
BclI	TGATCA	1 cut(s)	3361
BcnI	CCSGG	2 cut(s)	2708, 5326
BcoDI	GTCTC	6 cut(s)	40, 1748, 4137, 4838, 4894, 4989
BfmI	CTRYAG	5 cut(s)	273, 801, 5064, 5493, 5679
BfoI	RGCGCY	3 cut(s)	59, 144, 1863
BfrI	CTTAAG	1 cut(s)	827
BfuAI	ACCTGC	1 cut(s)	2836
BfuI	GTATCC	1 cut(s)	4852
BglI	GCCNNNNNGGC	1 cut(s)	149
BglII	AGATCT	1 cut(s)	3178
BlnI	CCTAGG	2 cut(s)	5020, 5099
BlpI	GCTNAGC	2 cut(s)	2346, 3860
BmcAI	AGTACT	2 cut(s)	3353, 3783
Bme18I	GGWCC	6 cut(s)	1124, 1665, 1810, 3106, 3391, 5437
BmeT110I	CYCGRG	2 cut(s)	3492, 4307
BmgT120I	GGNCC	9 cut(s)	1124, 1665, 1810, 2297, 3056, 3106, 3391, 3806, 5437
BmrI	ACTGGG	2 cut(s)	2516, 5587
BmtI	GCTAGC	1 cut(s)	1695
BmuI	ACTGGG	2 cut(s)	2516, 5587
BpiI	GAAGAC	6 cut(s)	167, 1286, 1775, 1989, 2052, 4389
BpmI	CTGGAG	2 cut(s)	1654, 5543
Bpu1102I	GCTNAGC	2 cut(s)	2346, 3860
Bpu14I	TTCGAA	1 cut(s)	5516
BpuEI	CTTGAG	4 cut(s)	806, 2289, 2904, 3723
BpuMI	CCSGG	2 cut(s)	2708, 5326
Bsa29I	ATCGAT	1 cut(s)	3826
BsaAI	YACGTR	4 cut(s)	647, 3917, 4303, 4576
BsaBI	GATNNNNATC	1 cut(s)	3243
BsaHI	GRCGYC	2 cut(s)	56, 141
BsaI	GGTCTC	1 cut(s)	1748
BsaWI	WCCGGW	2 cut(s)	2563, 5687
BsaXI	ACNNNNNCTCC	6 cut(s)	371, 401, 2889, 2919, 2988, 3018
Bsc4I	CCNNNNNNNGG	7 cut(s)	68, 346, 376, 1616, 2506, 4091, 5100
Bse118I	RCCGGY	3 cut(s)	148, 1384, 5687
Bse21I	CCTNAGG	1 cut(s)	3570
Bse3DI	GCAATG	4 cut(s)	2188, 2271, 3579, 4062
Bse8I	GATNNNNATC	1 cut(s)	3243
BseAI	TCCGGA	1 cut(s)	2563
BseCI	ATCGAT	1 cut(s)	3826
BseJI	GATNNNNATC	1 cut(s)	3243
BseLI	CCNNNNNNNGG	7 cut(s)	68, 346, 376, 1616, 2506, 4091, 5100
BseMI	GCAATG	4 cut(s)	2188, 2271, 3579, 4062
BseRI	GAGGAG	8 cut(s)	395, 914, 1939, 3072, 3189, 3313, 3359, 5583
BseSI	GKGCMC	3 cut(s)	1026, 3386, 3499
BseYI	CCCAGC	2 cut(s)	4487, 5260
BsgI	GTGCAG	4 cut(s)	215, 1875, 2947, 3543
Bsh1236I	CGCG	2 cut(s)	4683, 5355
BshNI	GGYRCC	5 cut(s)	55, 118, 140, 3496, 5024
BshTI	ACCGGT	1 cut(s)	5687
BshVI	ATCGAT	1 cut(s)	3826
BsiHKAI	GWGCWC	1 cut(s)	1026
BsiHKCI	CYCGRG	2 cut(s)	3492, 4307
BsiSI	CCGG	6 cut(s)	149, 1385, 2564, 2708, 5326, 5688
BsiWI	CGTACG	1 cut(s)	25
BslFI	GGGAC	7 cut(s)	1359, 1928, 2528, 2765, 3119, 3746, 5588
BslI	CCNNNNNNNGG	7 cut(s)	68, 346, 376, 1616, 2506, 4091, 5100
BsmAI	GTCTC	6 cut(s)	40, 1748, 4137, 4838, 4894, 4989
BsmBI	CGTCTC	1 cut(s)	40
BsmFI	GGGAC	7 cut(s)	1359, 1928, 2528, 2765, 3119, 3746, 5588
BsmI	GAATGC	2 cut(s)	2334, 3032
Bso31I	GGTCTC	1 cut(s)	1748
BsoBI	CYCGRG	2 cut(s)	3492, 4307
Bsp119I	TTCGAA	1 cut(s)	5516
Bsp1286I	GDGCHC	7 cut(s)	1026, 1739, 2535, 3386, 3499, 5164, 5667
Bsp13I	TCCGGA	1 cut(s)	2563
Bsp1407I	TGTACA	2 cut(s)	325, 3717
Bsp1720I	GCTNAGC	2 cut(s)	2346, 3860
Bsp19I	CCATGG	2 cut(s)	2442, 5146
BspACI	CCGC	8 cut(s)	5, 59, 3645, 4501, 4681, 4715, 4868, 5427
BspDI	ATCGAT	1 cut(s)	3826
BspEI	TCCGGA	1 cut(s)	2563
BspFNI	CGCG	2 cut(s)	4683, 5355
BspHI	TCATGA	4 cut(s)	445, 2758, 2929, 2938
BspMAI	CTGCAG	2 cut(s)	5068, 5683
BspMI	ACCTGC	1 cut(s)	2836
BspOI	GCTAGC	1 cut(s)	1695
BspQI	GCTCTTC	1 cut(s)	2659
BspT104I	TTCGAA	1 cut(s)	5516
BspT107I	GGYRCC	5 cut(s)	55, 118, 140, 3496, 5024
BspTI	CTTAAG	1 cut(s)	827
BspTNI	GGTCTC	1 cut(s)	1748
BsrDI	GCAATG	4 cut(s)	2188, 2271, 3579, 4062
BsrFI	RCCGGY	3 cut(s)	148, 1384, 5687
BsrGI	TGTACA	2 cut(s)	325, 3717
BssAI	RCCGGY	3 cut(s)	148, 1384, 5687
BssNAI	GTATAC	1 cut(s)	837
BssNI	GRCGYC	2 cut(s)	56, 141
BssSI	CACGAG	1 cut(s)	2054
BssT1I	CCWWGG	8 cut(s)	97, 2442, 2911, 3510, 4183, 5020, 5099, 5146
Bst1107I	GTATAC	1 cut(s)	837
Bst2BI	CACGAG	1 cut(s)	2054
Bst6I	CTCTTC	2 cut(s)	2659, 4992
BstACI	GRCGYC	2 cut(s)	56, 141
BstAFI	CTTAAG	1 cut(s)	827
BstAPI	GCANNNNNTGC	3 cut(s)	3581, 5072, 5601
BstAUI	TGTACA	2 cut(s)	325, 3717
BstBAI	YACGTR	4 cut(s)	647, 3917, 4303, 4576
BstBI	TTCGAA	1 cut(s)	5516
BstDSI	CCRYGG	4 cut(s)	2442, 2670, 3694, 5146
BstEII	GGTNACC	1 cut(s)	365
BstENI	CCTNNNNNAGG	1 cut(s)	344
BstFNI	CGCG	2 cut(s)	4683, 5355
BstH2I	RGCGCY	3 cut(s)	59, 144, 1863
BstHHI	GCGC	7 cut(s)	58, 143, 212, 1862, 2000, 3125, 5357
BstMAI	GTCTC	6 cut(s)	40, 1748, 4137, 4838, 4894, 4989
BstPI	GGTNACC	1 cut(s)	365
BstSFI	CTRYAG	5 cut(s)	273, 801, 5064, 5493, 5679
BstSLI	GKGCMC	3 cut(s)	1026, 3386, 3499
BstSNI	TACGTA	2 cut(s)	4303, 4576
BstUI	CGCG	2 cut(s)	4683, 5355
BstV2I	GAAGAC	6 cut(s)	167, 1286, 1775, 1989, 2052, 4389
BstX2I	RGATCY	7 cut(s)	1163, 2915, 3178, 3604, 4105, 4240, 5185
BstXI	CCANNNNNNTGG	4 cut(s)	702, 1580, 3659, 4885
BstYI	RGATCY	7 cut(s)	1163, 2915, 3178, 3604, 4105, 4240, 5185
BstZ17I	GTATAC	1 cut(s)	837
Bsu15I	ATCGAT	1 cut(s)	3826
Bsu36I	CCTNAGG	1 cut(s)	3570
BsuI	GTATCC	1 cut(s)	4852
BsuTUI	ATCGAT	1 cut(s)	3826
BtgI	CCRYGG	4 cut(s)	2442, 2670, 3694, 5146
BtsIMutI	CAGTG	8 cut(s)	1033, 1323, 2168, 2473, 2964, 4553, 4961, 5596
BveI	ACCTGC	1 cut(s)	2836
CaiI	CAGNNNCTG	4 cut(s)	1862, 2030, 4091, 4276
CciI	TCATGA	4 cut(s)	445, 2758, 2929, 2938
CfoI	GCGC	7 cut(s)	58, 143, 212, 1862, 2000, 3125, 5357
Cfr10I	RCCGGY	3 cut(s)	148, 1384, 5687
Cfr13I	GGNCC	9 cut(s)	1124, 1665, 1810, 2297, 3056, 3106, 3391, 3806, 5437
ClaI	ATCGAT	1 cut(s)	3826
CseI	GACGC	3 cut(s)	2651, 4659, 4940
CspAI	ACCGGT	1 cut(s)	5687
DinI	GGCGCC	2 cut(s)	57, 142
DraI	TTTAAA	1 cut(s)	4263
DrdI	GACNNNNNNGTC	2 cut(s)	591, 619
DseDI	GACNNNNNNGTC	2 cut(s)	591, 619
EaeI	YGGCCR	5 cut(s)	649, 4498, 4543, 4678, 5327
Eam1104I	CTCTTC	2 cut(s)	2659, 4992
EarI	CTCTTC	2 cut(s)	2659, 4992
Eco105I	TACGTA	2 cut(s)	4303, 4576
Eco130I	CCWWGG	8 cut(s)	97, 2442, 2911, 3510, 4183, 5020, 5099, 5146
Eco147I	AGGCCT	1 cut(s)	72
Eco24I	GRGCYC	4 cut(s)	1739, 2535, 5164, 5667
Eco31I	GGTCTC	1 cut(s)	1748
Eco32I	GATATC	5 cut(s)	79, 217, 681, 3824, 4458
Eco47I	GGWCC	6 cut(s)	1124, 1665, 1810, 3106, 3391, 5437
Eco57I	CTGAAG	6 cut(s)	984, 1509, 2053, 3306, 4185, 4973
Eco72I	CACGTG	1 cut(s)	3917
Eco81I	CCTNAGG	1 cut(s)	3570
Eco88I	CYCGRG	2 cut(s)	3492, 4307
Eco91I	GGTNACC	1 cut(s)	365
EcoNI	CCTNNNNNAGG	1 cut(s)	344
EcoO109I	RGGNCCY	4 cut(s)	1665, 1810, 2297, 3106
EcoO65I	GGTNACC	1 cut(s)	365
EcoRI	GAATTC	2 cut(s)	712, 1537
EcoRV	GATATC	5 cut(s)	79, 217, 681, 3824, 4458
EcoT14I	CCWWGG	8 cut(s)	97, 2442, 2911, 3510, 4183, 5020, 5099, 5146
EcoT22I	ATGCAT	2 cut(s)	2334, 5708
EcoT38I	GRGCYC	4 cut(s)	1739, 2535, 5164, 5667
EgeI	GGCGCC	2 cut(s)	57, 142
EheI	GGCGCC	2 cut(s)	57, 142
ErhI	CCWWGG	8 cut(s)	97, 2442, 2911, 3510, 4183, 5020, 5099, 5146
Esp3I	CGTCTC	1 cut(s)	40
FalI	AAGNNNNNCTT	8 cut(s)	152, 184, 1501, 1533, 1974, 2006, 2386, 2418
FaqI	GGGAC	7 cut(s)	1359, 1928, 2528, 2765, 3119, 3746, 5588
FauI	CCCGC	1 cut(s)	5420
FbaI	TGATCA	1 cut(s)	3361
FblI	GTMKAC	3 cut(s)	42, 836, 5557
FriOI	GRGCYC	4 cut(s)	1739, 2535, 5164, 5667
FspI	TGCGCA	1 cut(s)	1999
GlaI	GCGC	7 cut(s)	57, 142, 211, 1861, 1999, 3124, 5356
GsaI	CCCAGC	2 cut(s)	4491, 5264
GsuI	CTGGAG	2 cut(s)	1654, 5543
HaeII	RGCGCY	3 cut(s)	59, 144, 1863
HapII	CCGG	6 cut(s)	149, 1385, 2564, 2708, 5326, 5688
HgaI	GACGC	3 cut(s)	2651, 4659, 4940
HhaI	GCGC	7 cut(s)	58, 143, 212, 1862, 2000, 3125, 5357
Hin1I	GRCGYC	2 cut(s)	56, 141
Hin6I	GCGC	7 cut(s)	56, 141, 210, 1860, 1998, 3123, 5355
HinP1I	GCGC	7 cut(s)	56, 141, 210, 1860, 1998, 3123, 5355
HincII	GTYRAC	6 cut(s)	43, 484, 2823, 4359, 5173, 5558
HindII	GTYRAC	6 cut(s)	43, 484, 2823, 4359, 5173, 5558
HindIII	AAGCTT	7 cut(s)	15, 282, 356, 969, 1010, 1630, 2395
HpaI	GTTAAC	1 cut(s)	2823
HpaII	CCGG	6 cut(s)	149, 1385, 2564, 2708, 5326, 5688
Hpy99I	CGWCG	2 cut(s)	44, 1258
Hsp92I	GRCGYC	2 cut(s)	56, 141
HspAI	GCGC	7 cut(s)	56, 141, 210, 1860, 1998, 3123, 5355
KasI	GGCGCC	2 cut(s)	55, 140
KflI	GGGWCCC	1 cut(s)	3106
Kpn2I	TCCGGA	1 cut(s)	2563
KroI	GCCGGC	2 cut(s)	148, 1384
KroNI	GCCGGC	2 cut(s)	150, 1386
Ksp22I	TGATCA	1 cut(s)	3361
KspAI	GTTAAC	1 cut(s)	2823
LguI	GCTCTTC	1 cut(s)	2659
LmnI	GCTCC	3 cut(s)	1084, 1700, 5152
MaeIII	GTNAC	5 cut(s)	365, 879, 4465, 4787, 5049
MfeI	CAATTG	1 cut(s)	1395
MflI	RGATCY	7 cut(s)	1163, 2915, 3178, 3604, 4105, 4240, 5185
MhlI	GDGCHC	7 cut(s)	1026, 1739, 2535, 3386, 3499, 5164, 5667
MlsI	TGGCCA	1 cut(s)	651
MluNI	TGGCCA	1 cut(s)	651
Mly113I	GGCGCC	2 cut(s)	56, 141
MlyI	GAGTC	4 cut(s)	2066, 2510, 3310, 4097
MmeI	TCCRAC	4 cut(s)	1606, 2430, 3864, 4101
Mox20I	TGGCCA	1 cut(s)	651
Mph1103I	ATGCAT	2 cut(s)	2334, 5708
MroI	TCCGGA	1 cut(s)	2563
MroNI	GCCGGC	2 cut(s)	148, 1384
MroXI	GAANNNNTTC	4 cut(s)	1365, 1537, 1633, 5512
MscI	TGGCCA	1 cut(s)	651
MslI	CAYNNNNRTG	4 cut(s)	327, 513, 3657, 5180
Msp20I	TGGCCA	1 cut(s)	651
MspA1I	CMGCKG	4 cut(s)	229, 3136, 3645, 5063
MspCI	CTTAAG	1 cut(s)	827
MspI	CCGG	6 cut(s)	149, 1385, 2564, 2708, 5326, 5688
MunI	CAATTG	1 cut(s)	1395
Mva1269I	GAATGC	2 cut(s)	2334, 3032
MvnI	CGCG	2 cut(s)	4683, 5355
NaeI	GCCGGC	2 cut(s)	150, 1386
NarI	GGCGCC	2 cut(s)	56, 141
NciI	CCSGG	2 cut(s)	2708, 5326
NcoI	CCATGG	2 cut(s)	2442, 5146
NgoMIV	GCCGGC	2 cut(s)	148, 1384
NheI	GCTAGC	1 cut(s)	1691
NmeAIII	GCCGAG	1 cut(s)	4540
NmuCI	GTSAC	2 cut(s)	879, 5049
NsbI	TGCGCA	1 cut(s)	1999
NsiI	ATGCAT	2 cut(s)	2334, 5708
NspV	TTCGAA	1 cut(s)	5516
PaeI	GCATGC	1 cut(s)	4507
PagI	TCATGA	4 cut(s)	445, 2758, 2929, 2938
PaqCI	CACCTGC	1 cut(s)	2836
PceI	AGGCCT	1 cut(s)	72
PciI	ACATGT	3 cut(s)	514, 4862, 4923
PciSI	GCTCTTC	1 cut(s)	2659
PcsI	WCGNNNNNNNCGW	1 cut(s)	39
PctI	GAATGC	2 cut(s)	2334, 3032
PdiI	GCCGGC	2 cut(s)	150, 1386
PdmI	GAANNNNTTC	4 cut(s)	1365, 1537, 1633, 5512
Pfl23II	CGTACG	1 cut(s)	25
PflFI	GACNNNGTC	2 cut(s)	5177, 5579
PflMI	CCANNNNNTGG	2 cut(s)	2506, 4091
PfoI	TCCNGGA	1 cut(s)	2706
PinAI	ACCGGT	1 cut(s)	5687
PleI	GAGTC	4 cut(s)	2065, 2510, 3309, 4096
PluTI	GGCGCC	2 cut(s)	59, 144
PmaCI	CACGTG	1 cut(s)	3917
PmlI	CACGTG	1 cut(s)	3917
PpsI	GAGTC	4 cut(s)	2065, 2510, 3309, 4096
Ppu21I	YACGTR	4 cut(s)	647, 3917, 4303, 4576
PpuMI	RGGWCCY	3 cut(s)	1665, 1810, 3106
PscI	ACATGT	3 cut(s)	514, 4862, 4923
PsiI	TTATAA	2 cut(s)	128, 2106
Psp5II	RGGWCCY	3 cut(s)	1665, 1810, 3106
PspCI	CACGTG	1 cut(s)	3917
PspEI	GGTNACC	1 cut(s)	365
PspFI	CCCAGC	2 cut(s)	4487, 5260
PspLI	CGTACG	1 cut(s)	25
PspPI	GGNCC	9 cut(s)	1124, 1665, 1810, 2297, 3056, 3106, 3391, 3806, 5437
PspPPI	RGGWCCY	3 cut(s)	1665, 1810, 3106
PsrI	GAACNNNNNNTAC	4 cut(s)	292, 324, 795, 827
PstI	CTGCAG	2 cut(s)	5068, 5683
PstNI	CAGNNNCTG	4 cut(s)	1862, 2030, 4091, 4276
PsuI	RGATCY	7 cut(s)	1163, 2915, 3178, 3604, 4105, 4240, 5185
PsyI	GACNNNGTC	2 cut(s)	5177, 5579
PvuII	CAGCTG	3 cut(s)	229, 3136, 5063
RseI	CAYNNNNRTG	4 cut(s)	327, 513, 3657, 5180
SalI	GTCGAC	2 cut(s)	41, 5556
SapI	GCTCTTC	1 cut(s)	2659
Sau96I	GGNCC	9 cut(s)	1124, 1665, 1810, 2297, 3056, 3106, 3391, 3806, 5437
ScaI	AGTACT	2 cut(s)	3353, 3783
SchI	GAGTC	4 cut(s)	2066, 2510, 3310, 4097
SduI	GDGCHC	7 cut(s)	1026, 1739, 2535, 3386, 3499, 5164, 5667
SfcI	CTRYAG	5 cut(s)	273, 801, 5064, 5493, 5679
SfoI	GGCGCC	2 cut(s)	57, 142
SfuI	TTCGAA	1 cut(s)	5516
SinI	GGWCC	6 cut(s)	1124, 1665, 1810, 3106, 3391, 5437
SmiMI	CAYNNNNRTG	4 cut(s)	327, 513, 3657, 5180
SmlI	CTYRAG	5 cut(s)	785, 827, 2304, 2919, 3738
SmoI	CTYRAG	5 cut(s)	785, 827, 2304, 2919, 3738
SnaBI	TACGTA	2 cut(s)	4303, 4576
SphI	GCATGC	1 cut(s)	4507
SseBI	AGGCCT	1 cut(s)	72
SsiI	CCGC	8 cut(s)	5, 59, 3645, 4501, 4681, 4715, 4868, 5427
SspDI	GGCGCC	2 cut(s)	55, 140
SspI	AATATT	2 cut(s)	135, 4353
StuI	AGGCCT	1 cut(s)	72
StyI	CCWWGG	8 cut(s)	97, 2442, 2911, 3510, 4183, 5020, 5099, 5146
TatI	WGTACW	5 cut(s)	325, 3351, 3717, 3781, 4550
TauI	GCSGC	5 cut(s)	8, 61, 4503, 4683, 4871
TscAI	CASTG	8 cut(s)	1033, 1330, 2175, 2473, 2971, 4560, 4961, 5596
TseFI	GTSAC	2 cut(s)	879, 5049
Tsp45I	GTSAC	2 cut(s)	879, 5049
TspGWI	ACGGA	5 cut(s)	28, 43, 524, 2687, 3683
TspRI	CASTG	8 cut(s)	1033, 1330, 2175, 2473, 2971, 4560, 4961, 5596
Tth111I	GACNNNGTC	2 cut(s)	5177, 5579
Van91I	CCANNNNNTGG	2 cut(s)	2506, 4091
Vha464I	CTTAAG	1 cut(s)	827
VneI	GTGCAC	1 cut(s)	1022
VpaK11BI	GGWCC	6 cut(s)	1124, 1665, 1810, 3106, 3391, 5437
XagI	CCTNNNNNAGG	1 cut(s)	344
XapI	RAATTY	8 cut(s)	530, 712, 725, 998, 1537, 1658, 2721, 3312
XbaI	TCTAGA	1 cut(s)	3950
XcmI	CCANNNNNNNNNTGG	1 cut(s)	5153
XmaJI	CCTAGG	2 cut(s)	5020, 5099
XmiI	GTMKAC	3 cut(s)	42, 836, 5557
XmnI	GAANNNNTTC	4 cut(s)	1365, 1537, 1633, 5512
ZrmI	AGTACT	2 cut(s)	3353, 3783
Zsp2I	ATGCAT	2 cut(s)	2334, 5708

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.