Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0004063

Physical Location & Seq

Genomic Coordinates Reverse (-)

45442543 .. 45453033

Gene Structure 10491 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025385

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5889 bp

ATGCCGCCTTCCGACGGCTTCAGCGTCCGGATCGTCTCCGTCGATTTCTACATGGCGACGCCCATCCCTGGCCTCGATATCTCCTACAGTAGCTTCCACGGTGGAAAGGTGAATGAGGTACCTGTAATAAGAATTTACGGCTCTACCCCGGCCGGTCAGAAAGCTTGTCTGCACATTCACAGAGCATTACCATACTTATTTGTGCCGTGCAAAGATATACCGATTCAACCACATCAGAAAGGTGATTCGTACACATATGATATATCTCTTGCTTTAGAGAAAGCTTTGAAGCTCAAAAGCAATGCTGGTTCAAAGCGACAACATGTACATGGTTGCAGCCTGGTCCAGGCAAGGAAGCTTTACGGTTACCATTCATCAGAGGAGGTGTTTGTGAAGATATACCTCTATTATCCACATGATGTGACTCGCGCAGCCAATCTTCTTCTGGGAGGTGCAGTTCTTGATAAATGCTTACAGCCCCACGAATCACACATTCCCTTCATTCTCCAATTTATGATTGACTACAACTTGTATGGGATGGGTCATCTGCATCTATCAAAATTGAAGTTTCGTCATCCAATGCCAGATGCTTTTACCCCAAGGAGATCTTTTTACAATGGACTGCCCGGTCAGGACATAGATAATTTAACTGGGATGTCTACAGATGCTCAGGCAGATATTAGTGACCATCTGCTTTTTGGTTCACCGGTTTGGATATCGTCCACAATTCCAGTAGACTGGACATGGCTTTCTCCTGGTGATGTTGAGGCTTCATCGAATAGAGATGCTAACTGCACTAAACGACAAAGTATTTGTGAACTCGAGGGGGATGCTACTGTAGATGAGATACTCAATCAGCAGTTCAAAATGTATACATCCCTCTTGCAAACTCGTTCTGATGTGAAAATGGTTCAATCGCTTATACCAATTTGGGAGGAGTTTGAGAGAAATGGGATACACGAGGCAGCAATATCTTCTAATCCTGCTAAACCACTTCCGGAAGATGTTTTGAAAACTTTATCACTTGGTCCAGAATTTAATGAGAAACTTGTCAAATTGCATTTTGATACAGAAAACTCTTTGCCTTTAACTCAATTAAAGAAAGATGTAAAGTCTATGCACCAAATGACGTCTCCAAAAGATGGAGTAAATTCTGTTAAACTTCGAACTGATCTCAATTATGGAGAGATGATGGGATCTCTGTTGACACAAGTTTCTACTGATGAGACGGATGCAAAACTGAATAAAGAACTGCCAGCATCTCAAGCGATTCAGACATTAAGTATGAAGGCTGCAGATAATGAAGCACTAGCTCTGCTTAGATGGCTTGCCACTTCTCAGGCAGCTGATGATTTGAACTCCGATGATGAACTTGTTCAGGAGACAATACTGAGTCCTTTGTTGCCTGCAACAACTATTGACAAAGTTCTGGAGAAAGCTAATATGGATTATGAGAGTGAATCCCAAAAGGAATGTCAGGACATCCTTGATTCAGTTGGCGACTTTGAGGGATCAAAGGAAAAAGATTCTTATCCCACTGATCGGAACTGTTCTTCCAAAAGTGAACCTGCATGTATGGTTCCCCAGGTGAACGGTACTGGGGATGATGTTCTCTCAACTCCATCTCCTTGTGCCGGATCAATCGAAAATATTTCTGAAGTAGAGATGAAGAATGAATTTAGGACTCCTGAACCCCAGGTTACGAAAGATGGTCTAAGCTCCAATCATAAACACAAAAGGAAAAAATTATTATGGGGCTCTTTACCTTTGTCTGCGGCCCAAAAGCTGAAGACTGCGAGTGAACCTGTCAGTTTGTACAAGGAAACTAAGGATCCTGTTGGTACAAGTTCCTCCTCTGAAAGTGAAGTGAGAAAACATGCAGGCACTGATGCTTGTGATAGTACGTTGGGAAGATGCTCTCTGCGGGACTTGATGAGGAGAAAGCGATCTTATCGAATTGAGCCACCTGAAAAAGAAGATACACTTTATCAAATGTTGCATTTTGACGAAATTAATGGGAAGTCTCGTGAGTCTTTAGCTGACAGGCTATCATTAGCTCACCAACACTCCGTAAGTCATGAGGCTTATGATGTTAAAACAACCAACTCTGGCTGTTCCTTGTATGGTAAACTTCCACTTTTGTCTGGCAGTGTCAACTCTTTAAAGGCAAGTACGTTAGATGGAAAATTTGACACAAATATCAAAATTGCTGCAGCAGTTGGCACTGGAGCAATTTCTCAACATGGGAACATCTTCACAGGCCCTTCTGAAGCTCCGCCTGTGAATACAGGATTCTGGCAATTAAAGCCTGACAAGCTCGAGTCTGAAGCTTCCCTTGGCCGTTGTGAAACTTTCAATAAATCTAATGCTCAGACTTCCAAGTCAGGTGCTGAGGAAAACTTTTTTGTTGATGAGAGTTTTCATAGTTCTGCTGCTGACCCTGAAGTTCCTTGTGGAGATGGTCACACTGGAAGGGGCATTGATGGCTTATCTTCCAGAAGTGGAGTATCCGAGATACCTTTTGAGATTGGCACAAATGTCACAACTGACAAAACTGCTTTAAACAATGAAAGGTGTGGTAGCCAGAAACTGGGGAGAGATTCCTTTTCATCTGGGCTTAGTAACTTGTTGCCCTCGGTCATCCAGTCTGATGATAAGGACTTAATTGGCATGACCTTCGCTAGGAAACCCCCAATTGCAGACCGGAAGGATGGGGCATTTCTGAGTTCTTCATATTTACAAGAGGAAGGAGCTTTGGATGATATTCTCCCATTTTTTGTGAGAGACTGCCAAAATGAGACAGACATCGATAACAATTGCGTTAGAAATAGTGAGTCTCATTCTCATCAAGAATCTGCCTTAGGTGTCCCTACTCACTATCAAGCTGATGGCTCTTGTTTGTATCTGTTAACACCTGCAATTTCACCTCCTTCTGCCAAAAATGTATACCGCTGGCTGTCAAGAGGCAACAAAGGATCTTCTGCGAGTCTGACTGGTTCACAGAATTGTTTGCCTAATGATTTGGACAAGCCTTCACTCAAATATGGTTCTGTATGTGATGTGCAACCTAATCTACCCCAAGGACCTCAAGTAGATCATAGAAATCTTGAAGCAGAAAGGATTCCTTTTTGTAATGACATGACAGAAGTATTGGAAAGAGAAGCCGATGTTATAAGAGTTAAAACATACCCTGACCGCTCACAAGATAGTTCTCAGATTTCAGGTCCAGATGGGAGATCAAAATCCACTCCGCTTAGTCAAACTGGATTTCGGGACCCGGCAAGTGTTGGCATTGGCCAGCAGCTGACGTTGTTAAGTATAGAGGTTCAAGCAGAATCTAGGGGTGATCTTCGACCCGATCCTCAGTTTGATGCTATCAATATTGTATTGCTTGCTATTCAGAATGATTGTGACTCCATCGTTGAAGTTCATGTACTTATGCGCAGCGATGCTGAAAATTGTCAGAGGAGTCATCACGGGATTTCTGACTGCAAGCTGTTTGTGTTTCATGAAGAGAAGTACTTATTTCATCATTTTATCAAGTTTGTATGCTCGTTGGACCCTGATATATTAATGGGTTGGGATATTCAAGGTGGTTCCCTTGGTTTTTTGGCTGAAAGGGCTTCACATCTTGGTATAGGCTTGCTCAACAAGATATCTAGAACACCATCTGAAGCCAAGATGGTGGCTGAGGATTCAGAAGTTCTGCAGAAAGTCTTACAAGATAACCTGGCTGCTGACTCAAACATTGCTGATTCAGTTGTATTAGAAGATCCAATAATTGAGGATGAATGGGGACGGACTCATTCCAGCGGTGTCCATGTTGGTGGTAGAATAGTACTTAATGTCTGGCGGCTGATGCGCAGTGAAGTGAAGCTTAATATGTACACAGTTGAAGCTGTTTCTGAAGTTGTGTTGAGGCGGAAAGTCCCATATATTCCTAATAAACTACTCACAAAATGGTTTTTAAGTGGTCCTGGAAGAGCAAGATACCGATGTATCAAATATGTGAAGGAGAGAGCAGAATTAAACCTTGAGATGATGAATCAATTAGATATGATAAATCGAACATCAGAACTTGCACGTGTCTTTGGCATTGACTTTTTTTCTGTTCTCTCTAGAGGCTCCCAATACCGTGTTGAATCTATGGTTCTGAGATTGGCACATACACAAAACTATCTTGCCATTTCCCCTGGGAAACAACAGGTAGCTTCGCAGCCAGCAATGGAATGCATCCCTCTTGTAATGGAACCAGAATCTGGTTTTTATGCAGATCCAGTAGTTGTTTTGGATTTTCAGTCTCTTTATCCATCAATGATAATTGCATACAACCTTTGCTATTCTACATGCCTTGGAAAAGTTGCACCTTCAGAGCCAAATACACTTGGTGTCAGTTCATTTTCACCAGATCCACATGATCTGCATGATTTAAAAGATCAAATACTACTTACTCCAAATGGTGTTATGTGTGTGCCAGCGAAGGTCCGTAAAGGCGTACTGCCACGCTTATTGGACGAAATATTGTCAACCAGAATAATGGTTAAACAAGCGATGAAAAAATTGTCTCCATCACAGAAAGTTCTTCACAGGATATTTAATGCAAGACAGCTTGCACTAAAGCTGATAGCAAACGTGACGTATGGTTATACAGCTGCTGGATTTAGTGGCCGCATGCCTTGTGCAGAGATTGCAGACAGTATTGTTCAGTGTGGCCGTAGCACACTTGAAAAGGCTATTTCATATGTAAATGCACATGACAGATGGAAGGCTAGAGTTATTTATGGCGACACAGACAGCATGTTTGTCCTTCTAAAAGGCCGCACTAAGGAAGAGTCTTTTCAGATTGGACAGGAGATTGCATCTGCAATCAGTACTATAAGTCCAACTCCAGTTGCTTTGAAGATGGAAAAAGTTTATCAGCCATGCTTCCTCCTTACCAAGAAGCGATATGTTGGTTACAGTTATGAAAGCCCTGGACAGAGTGAACCTATTTTTGATGCTAAAGGCATTGAGACAGTTCGCAGGGATACATGTGGGGCTGTTGCCAAGACAATGGAGCAGTCTCTGAGACTTTTTTTTGAACATCAAGACATGCCTGAGGTCAAAGCATACTTACAGCGCCAGTGGAAGCGAATTCTTTCTGGAAGAGTGTCCCTTCAGGATTTTGTATTTGCAAAAGAGGTTCGTTTAGGAACTTACCGTTCTAGTGGTTTATCACTTCCACCAGCTGCAATTGTTGCTACGAAAGCTATGAGAATTGACCCTAGGGCAGAACCACGTTATGCAGAGAGGGTACCTTACGTTGTAATACATGGGGAGCCTGGGGCCCGTCTGGCTGATGTGGTTGTGGATCCTTTGAACCTCCTGGCCACTGATTCACCCTATAGACTAAATGATCTCTATTACATCCAAAAGCAGATAATCCCAGCTTTGCAGCGAGTGTTTGGGCTTGTTGGAGCCGACCTGCAGCAGTGGTTTTCAGATATGCCCCGCCCAGTAAGGGAAGCTTTTGGAAAACGCCCCTCTTATGCTTCAAATCCACACCGAACCAGAATTGACTATTACTATCTTTCAAGGCATTGCATATTGTGTGGTGAGTTGGTCCAGGGATCAGCCCATCTATGCAATCAATGTGCTGAAAACAAAACTGCTGCAGCAGCAGCTATAATCGGAAGAACTTCAAAGCTGGAGAGAGAGATGCAACACCTTGCTGCTATCTGTCGACATTGTGGAGGTGGGGATGCGCTTGAAGGAAATGGCATCAAATGCACTTCTCTTGCATGCTCAGTGTTTTATGAGAGACGCAAAGTTCAGAAAGAACTGCAGGGAACTGCTTCTGTTGCTGCAGAAACAGGTTTGTACCCTCAATGTTTGGTCGAGTGGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000731 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010225 GO:0016035 GO:0018130 GO:0019438 GO:0019985 GO:0032991 GO:0033554 GO:0034641 GO:0034644 GO:0034645 GO:0034654 GO:0042276 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0071214 GO:0071478 GO:0071482 GO:0071494 GO:0071704 GO:0071897 GO:0090304 GO:0104004 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko01100 ko01524 ko03460 map01100 map01524 map03460

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 RVT_2 zf-C4pol zf-RanBP

Protein Analysis

1963

Amino Acids

217.3

Weight (kDa)

6.07

Isoelectric Point (pI)

45.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
REV3_N	PF24065	7 - 60	4.9e-18	DNA polymerase zeta catalytic subunit, N-terminal
POL3_N	PF24055	62 - 141	3.2e-22	DNA polymerase delta catalytic subunit-like, N-terminal domain
DNA_pol_B_exo1	PF03104	1100 - 1229	3.6e-09	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	1368 - 1815	4.7e-100	DNA polymerase family B
zf-C4pol	PF14260	1861 - 1934	2.3e-14	C4-type zinc-finger of DNA polymerase delta

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004063)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500
fragaria_vesca	FvH4_6g06120 FvH4_6g06120 FvH4_6g06120 FvH4_6g06120 FvH4_6g06120
malus_domestica	MD05G1053400.v1.1 MD05G1053600.v1.1 MD05G1053700.v1.1 MD12G1217900.v1.1
prunus_persica	Prupe.6G324000_v2.0.a1 Prupe.6G324000_v2.0.a1 Prupe.6G324000_v2.0.a1
pyrus_communis	pycom05g04420 pycom12g20340
rosa_chinensis	RchiOBHm_Chr3g0455131
rosa_laevigata	RLG00000025385
rosa_multiflora	Rmu_co8200746.1_g000001 Rmu_sc0041669.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424540
rosa_rugosa	Rorug01G0381700 Rorug03G0004000
rosa_samantha	Rh3AG064900 Rh3BG066900 Rh3CG065300 Rh3DG066800
rosa_wichuraiana	Rw3G005080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3173
AarI	CACCTGC	1 cut(s)	2923
AasI	GACNNNNNNGTC	2 cut(s)	627, 2380
AatII	GACGTC	1 cut(s)	1133
Acc16I	TGCGCA	2 cut(s)	3443, 3863
Acc36I	ACCTGC	3 cut(s)	1576, 2923, 5472
Acc65I	GGTACC	2 cut(s)	118, 5293
AccB1I	GGYRCC	2 cut(s)	118, 5293
AccB7I	CCANNNNNTGG	3 cut(s)	1142, 2590, 4259
AccBSI	CCGCTC	1 cut(s)	3198
AccI	GTMKAC	5 cut(s)	659, 735, 872, 2946, 5722
AccII	CGCG	1 cut(s)	429
AccIII	TCCGGA	2 cut(s)	27, 997
AcoI	YGGCCR	6 cut(s)	150, 2338, 3295, 4666, 4711, 5367
AcsI	RAATTY	6 cut(s)	132, 1034, 1150, 1676, 2186, 5133
AcvI	CACGTG	1 cut(s)	4085
AcyI	GRCGYC	2 cut(s)	59, 1130
AdeI	CACNNNGTG	1 cut(s)	4388
AflIII	ACRYGT	3 cut(s)	322, 4084, 5030
AgeI	ACCGGT	1 cut(s)	706
AjuI	GAANNNNNNNTTGG	2 cut(s)	2319, 2351
AloI	GAACNNNNNNTCC	4 cut(s)	441, 473, 1538, 1570
AlwNI	CAGNNNCTG	7 cut(s)	1886, 2390, 2590, 3304, 4259, 4655, 5065
Ama87I	CYCGRG	2 cut(s)	821, 2318
Aor13HI	TCCGGA	2 cut(s)	27, 997
ApaI	GGGCCC	1 cut(s)	5329
ApoI	RAATTY	6 cut(s)	132, 1034, 1150, 1676, 2186, 5133
ArsI	GACNNNNNNTTYG	6 cut(s)	795, 827, 1459, 1491, 4784, 4816
AseI	ATTAAT	2 cut(s)	2013, 3572
AsiGI	ACCGGT	1 cut(s)	706
Asp700I	GAANNNNTTC	6 cut(s)	1374, 2252, 3120, 5137, 5677, 5833
Asp718I	GGTACC	2 cut(s)	118, 5293
AspA2I	CCTAGG	1 cut(s)	5264
AspLEI	GCGC	5 cut(s)	431, 3444, 3864, 5121, 5746
AsuC2I	CCSGG	3 cut(s)	149, 627, 3278
AsuII	TTCGAA	1 cut(s)	1165
AvaI	CYCGRG	2 cut(s)	821, 2318
AvaII	GGWCC	9 cut(s)	343, 1028, 3083, 3224, 3274, 3559, 3974, 4483, 5602
AvrII	CCTAGG	1 cut(s)	5264
AxyI	CCTNAGG	2 cut(s)	2860, 5097
BaeGI	GKGCMC	1 cut(s)	5329
BaeI	ACNNNNGTAYC	4 cut(s)	3982, 3982, 4015, 4015
BalI	TGGCCA	2 cut(s)	3297, 5369
BamHI	GGATCC	2 cut(s)	1831, 5350
BanI	GGYRCC	2 cut(s)	118, 5293
BanII	GRGCYC	2 cut(s)	1760, 5329
BarI	GAAGNNNNNNTAC	2 cut(s)	3417, 3449
BauI	CACGAG	2 cut(s)	959, 2025
BbrPI	CACGTG	1 cut(s)	4085
BbsI	GAAGAC	1 cut(s)	1796
BbvCI	CCTCAGC	2 cut(s)	2391, 3690
BceAI	ACGGC	5 cut(s)	31, 154, 190, 2325, 4698
BcgI	CGANNNNNNTGC	4 cut(s)	812, 846, 2659, 2693
BciVI	GTATCC	3 cut(s)	948, 2518, 5020
BcnI	CCSGG	3 cut(s)	149, 627, 3278
BfaI	CTAG	8 cut(s)	1310, 2682, 3339, 3660, 4119, 4770, 5205, 5265
BfoI	RGCGCY	1 cut(s)	5122
BfuAI	ACCTGC	3 cut(s)	1576, 2923, 5472
BfuI	GTATCC	3 cut(s)	948, 2518, 5020
BglI	GCCNNNNNGGC	1 cut(s)	5333
BglII	AGATCT	1 cut(s)	605
BlnI	CCTAGG	1 cut(s)	5264
BmcAI	AGTACT	3 cut(s)	3521, 3840, 4873
Bme18I	GGWCC	9 cut(s)	343, 1028, 3083, 3224, 3274, 3559, 3974, 4483, 5602
BmeT110I	CYCGRG	2 cut(s)	821, 2318
BmrI	ACTGGG	4 cut(s)	660, 1608, 2600, 5489
BmuI	ACTGGG	4 cut(s)	660, 1608, 2600, 5489
BpiI	GAAGAC	1 cut(s)	1796
BpmI	CTGGAG	4 cut(s)	1451, 2247, 4872, 5708
Bpu10I	CCTNAGC	3 cut(s)	669, 2391, 3690
Bpu14I	TTCGAA	1 cut(s)	1165
BpuEI	CTTGAG	3 cut(s)	1248, 3072, 4055
BpuMI	CCSGG	3 cut(s)	149, 627, 3278
Bsa29I	ATCGAT	1 cut(s)	2808
BsaAI	YACGTR	1 cut(s)	4085
BsaBI	GATNNNNATC	2 cut(s)	1530, 3241
BsaHI	GRCGYC	2 cut(s)	59, 1130
BsaWI	WCCGGW	4 cut(s)	27, 706, 997, 2703
BsaXI	ACNNNNNCTCC	6 cut(s)	441, 471, 1609, 1639, 2051, 2081
Bse118I	RCCGGY	2 cut(s)	152, 706
Bse21I	CCTNAGG	2 cut(s)	2860, 5097
Bse3DI	GCAATG	4 cut(s)	307, 3747, 4230, 5578
Bse8I	GATNNNNATC	2 cut(s)	1530, 3241
BseAI	TCCGGA	2 cut(s)	27, 997
BseCI	ATCGAT	1 cut(s)	2808
BseJI	GATNNNNATC	2 cut(s)	1530, 3241
BseMI	GCAATG	4 cut(s)	307, 3747, 4230, 5578
BseRI	GAGGAG	5 cut(s)	395, 950, 1843, 1951, 3481
BseSI	GKGCMC	1 cut(s)	5329
BseX3I	CGGCCG	1 cut(s)	150
BseYI	CCCAGC	1 cut(s)	5425
BsgI	GTGCAG	4 cut(s)	155, 474, 778, 4701
Bsh1236I	CGCG	1 cut(s)	429
Bsh1285I	CGRYCG	1 cut(s)	153
BshNI	GGYRCC	2 cut(s)	118, 5293
BshTI	ACCGGT	1 cut(s)	706
BshVI	ATCGAT	1 cut(s)	2808
BsiEI	CGRYCG	1 cut(s)	153
BsiHKCI	CYCGRG	2 cut(s)	821, 2318
BsiSI	CCGG	9 cut(s)	28, 149, 153, 627, 707, 998, 1635, 2704, 3278
BslFI	GGGAC	6 cut(s)	1940, 2852, 3287, 3810, 3914, 5137
BsmBI	CGTCTC	4 cut(s)	40, 1137, 1220, 5794
BsmFI	GGGAC	6 cut(s)	1940, 2852, 3287, 3810, 3914, 5137
BsmI	GAATGC	1 cut(s)	4235
BsoBI	CYCGRG	2 cut(s)	821, 2318
Bsp119I	TTCGAA	1 cut(s)	1165
Bsp120I	GGGCCC	1 cut(s)	5325
Bsp1286I	GDGCHC	2 cut(s)	1760, 5329
Bsp13I	TCCGGA	2 cut(s)	27, 997
Bsp1407I	TGTACA	3 cut(s)	325, 1815, 3885
BspDI	ATCGAT	1 cut(s)	2808
BspEI	TCCGGA	2 cut(s)	27, 997
BspFNI	CGCG	1 cut(s)	429
BspHI	TCATGA	2 cut(s)	2077, 3508
BspMAI	CTGCAG	7 cut(s)	1297, 2215, 3711, 5469, 5656, 5826, 5848
BspMI	ACCTGC	3 cut(s)	1576, 2923, 5472
BspQI	GCTCTTC	1 cut(s)	3976
BspT104I	TTCGAA	1 cut(s)	1165
BspT107I	GGYRCC	2 cut(s)	118, 5293
BsrBI	CCGCTC	1 cut(s)	3198
BsrDI	GCAATG	4 cut(s)	307, 3747, 4230, 5578
BsrFI	RCCGGY	2 cut(s)	152, 706
BsrGI	TGTACA	3 cut(s)	325, 1815, 3885
BssAI	RCCGGY	2 cut(s)	152, 706
BssNAI	GTATAC	2 cut(s)	873, 2947
BssNI	GRCGYC	2 cut(s)	59, 1130
BssSI	CACGAG	2 cut(s)	959, 2025
BssT1I	CCWWGG	6 cut(s)	599, 2335, 3079, 3601, 4351, 5264
Bst1107I	GTATAC	2 cut(s)	873, 2947
Bst2BI	CACGAG	2 cut(s)	959, 2025
Bst6I	CTCTTC	4 cut(s)	3507, 3976, 4824, 5140
BstACI	GRCGYC	2 cut(s)	59, 1130
BstAPI	GCANNNNNTGC	3 cut(s)	4688, 5237, 5766
BstAUI	TGTACA	3 cut(s)	325, 1815, 3885
BstBAI	YACGTR	1 cut(s)	4085
BstBI	TTCGAA	1 cut(s)	1165
BstDSI	CCRYGG	1 cut(s)	97
BstEII	GGTNACC	1 cut(s)	365
BstENI	CCTNNNNNAGG	2 cut(s)	344, 2680
BstFNI	CGCG	1 cut(s)	429
BstH2I	RGCGCY	1 cut(s)	5122
BstHHI	GCGC	5 cut(s)	431, 3444, 3864, 5121, 5746
BstMCI	CGRYCG	1 cut(s)	153
BstNSI	RCATGY	9 cut(s)	326, 1575, 1880, 4350, 4675, 4801, 5034, 5095, 5784
BstPI	GGTNACC	1 cut(s)	365
BstSLI	GKGCMC	1 cut(s)	5329
BstUI	CGCG	1 cut(s)	429
BstV2I	GAAGAC	1 cut(s)	1796
BstX2I	RGATCY	8 cut(s)	605, 1196, 1831, 2975, 3772, 4273, 4408, 5350
BstXI	CCANNNNNNTGG	5 cut(s)	738, 3685, 3827, 4537, 5053
BstYI	RGATCY	8 cut(s)	605, 1196, 1831, 2975, 3772, 4273, 4408, 5350
BstZ17I	GTATAC	2 cut(s)	873, 2947
BstZI	CGGCCG	1 cut(s)	150
Bsu15I	ATCGAT	1 cut(s)	2808
Bsu36I	CCTNAGG	2 cut(s)	2860, 5097
BsuI	GTATCC	3 cut(s)	948, 2518, 5020
BsuTUI	ATCGAT	1 cut(s)	2808
BtgI	CCRYGG	1 cut(s)	97
BtgZI	GCGATG	2 cut(s)	3462, 4565
BtsI	GCAGTG	3 cut(s)	2155, 3871, 5477
BveI	ACCTGC	3 cut(s)	1576, 2923, 5472
CaiI	CAGNNNCTG	7 cut(s)	1886, 2390, 2590, 3304, 4259, 4655, 5065
CciI	TCATGA	2 cut(s)	2077, 3508
CfoI	GCGC	5 cut(s)	431, 3444, 3864, 5121, 5746
Cfr10I	RCCGGY	2 cut(s)	152, 706
ClaI	ATCGAT	1 cut(s)	2808
CseI	GACGC	3 cut(s)	13, 67, 5811
CspAI	ACCGGT	1 cut(s)	706
CspCI	CAANNNNNGTGG	2 cut(s)	4491, 4526
DraI	TTTAAA	3 cut(s)	2163, 2562, 4431
DraIII	CACNNNGTG	1 cut(s)	4388
DrdI	GACNNNNNNGTC	2 cut(s)	627, 2380
DseDI	GACNNNNNNGTC	2 cut(s)	627, 2380
EaeI	YGGCCR	6 cut(s)	150, 2338, 3295, 4666, 4711, 5367
EagI	CGGCCG	1 cut(s)	150
Eam1104I	CTCTTC	4 cut(s)	3507, 3976, 4824, 5140
EarI	CTCTTC	4 cut(s)	3507, 3976, 4824, 5140
EciI	GGCGGA	2 cut(s)	2265, 3937
EclXI	CGGCCG	1 cut(s)	150
Eco130I	CCWWGG	6 cut(s)	599, 2335, 3079, 3601, 4351, 5264
Eco24I	GRGCYC	2 cut(s)	1760, 5329
Eco32I	GATATC	3 cut(s)	79, 717, 3657
Eco47I	GGWCC	9 cut(s)	343, 1028, 3083, 3224, 3274, 3559, 3974, 4483, 5602
Eco52I	CGGCCG	1 cut(s)	150
Eco72I	CACGTG	1 cut(s)	4085
Eco81I	CCTNAGG	2 cut(s)	2860, 5097
Eco88I	CYCGRG	2 cut(s)	821, 2318
Eco91I	GGTNACC	1 cut(s)	365
EcoNI	CCTNNNNNAGG	2 cut(s)	344, 2680
EcoO109I	RGGNCCY	4 cut(s)	2261, 3083, 3274, 5325
EcoO65I	GGTNACC	1 cut(s)	365
EcoRI	GAATTC	1 cut(s)	5133
EcoRV	GATATC	3 cut(s)	79, 717, 3657
EcoT14I	CCWWGG	6 cut(s)	599, 2335, 3079, 3601, 4351, 5264
EcoT22I	ATGCAT	1 cut(s)	4235
EcoT38I	GRGCYC	2 cut(s)	1760, 5329
ErhI	CCWWGG	6 cut(s)	599, 2335, 3079, 3601, 4351, 5264
Esp3I	CGTCTC	4 cut(s)	40, 1137, 1220, 5794
FaqI	GGGAC	6 cut(s)	1940, 2852, 3287, 3810, 3914, 5137
FauI	CCCGC	2 cut(s)	1917, 5498
FauNDI	CATATG	2 cut(s)	256, 4741
FblI	GTMKAC	5 cut(s)	659, 735, 872, 2946, 5722
FriOI	GRGCYC	2 cut(s)	1760, 5329
FspBI	CTAG	8 cut(s)	1310, 2682, 3339, 3660, 4119, 4770, 5205, 5265
FspI	TGCGCA	2 cut(s)	3443, 3863
GlaI	GCGC	5 cut(s)	430, 3443, 3863, 5120, 5745
GsaI	CCCAGC	1 cut(s)	5429
GsuI	CTGGAG	4 cut(s)	1451, 2247, 4872, 5708
HaeII	RGCGCY	1 cut(s)	5122
HapII	CCGG	9 cut(s)	28, 149, 153, 627, 707, 998, 1635, 2704, 3278
HgaI	GACGC	3 cut(s)	13, 67, 5811
HhaI	GCGC	5 cut(s)	431, 3444, 3864, 5121, 5746
Hin1I	GRCGYC	2 cut(s)	59, 1130
Hin6I	GCGC	5 cut(s)	429, 3442, 3862, 5119, 5744
HinP1I	GCGC	5 cut(s)	429, 3442, 3862, 5119, 5744
HincII	GTYRAC	5 cut(s)	1206, 2155, 2910, 4527, 5723
HindII	GTYRAC	5 cut(s)	1206, 2155, 2910, 4527, 5723
HindIII	AAGCTT	6 cut(s)	162, 282, 356, 2328, 3875, 5505
HpaI	GTTAAC	1 cut(s)	2910
HpaII	CCGG	9 cut(s)	28, 149, 153, 627, 707, 998, 1635, 2704, 3278
Hpy99I	CGWCG	3 cut(s)	17, 44, 61
HpyCH4IV	ACGT	9 cut(s)	1130, 1904, 2174, 3308, 4084, 4632, 4637, 5278, 5301
HpySE526I	ACGT	9 cut(s)	1130, 1904, 2174, 3308, 4084, 4632, 4637, 5278, 5301
Hsp92I	GRCGYC	2 cut(s)	59, 1130
HspAI	GCGC	5 cut(s)	429, 3442, 3862, 5119, 5744
KflI	GGGWCCC	1 cut(s)	3274
Kpn2I	TCCGGA	2 cut(s)	27, 997
KpnI	GGTACC	2 cut(s)	122, 5297
KspAI	GTTAAC	1 cut(s)	2910
LguI	GCTCTTC	1 cut(s)	3976
LmnI	GCTCC	8 cut(s)	1724, 2228, 2278, 2750, 4130, 5056, 5317, 5456
MaeI	CTAG	8 cut(s)	1310, 2682, 3339, 3660, 4119, 4770, 5205, 5265
MaeII	ACGT	9 cut(s)	1130, 1904, 2174, 3308, 4084, 4632, 4637, 5278, 5301
MbiI	CCGCTC	1 cut(s)	3198
MfeI	CAATTG	3 cut(s)	2694, 2815, 5232
MflI	RGATCY	8 cut(s)	605, 1196, 1831, 2975, 3772, 4273, 4408, 5350
MhlI	GDGCHC	2 cut(s)	1760, 5329
MlsI	TGGCCA	2 cut(s)	3297, 5369
MluNI	TGGCCA	2 cut(s)	3297, 5369
MmeI	TCCRAC	4 cut(s)	36, 3537, 4907, 5434
Mox20I	TGGCCA	2 cut(s)	3297, 5369
Mph1103I	ATGCAT	1 cut(s)	4235
MroI	TCCGGA	2 cut(s)	27, 997
MroXI	GAANNNNTTC	6 cut(s)	1374, 2252, 3120, 5137, 5677, 5833
MscI	TGGCCA	2 cut(s)	3297, 5369
MslI	CAYNNNNRTG	2 cut(s)	327, 3825
Msp20I	TGGCCA	2 cut(s)	3297, 5369
MspA1I	CMGCKG	6 cut(s)	1346, 2952, 3304, 3813, 4652, 5228
MspI	CCGG	9 cut(s)	28, 149, 153, 627, 707, 998, 1635, 2704, 3278
MunI	CAATTG	3 cut(s)	2694, 2815, 5232
Mva1269I	GAATGC	1 cut(s)	4235
MvnI	CGCG	1 cut(s)	429
NciI	CCSGG	3 cut(s)	149, 627, 3278
NdeI	CATATG	2 cut(s)	256, 4741
NmuCI	GTSAC	6 cut(s)	421, 683, 2462, 2539, 3410, 4633
NsbI	TGCGCA	2 cut(s)	3443, 3863
NsiI	ATGCAT	1 cut(s)	4235
NspI	RCATGY	9 cut(s)	326, 1575, 1880, 4350, 4675, 4801, 5034, 5095, 5784
NspV	TTCGAA	1 cut(s)	1165
PaeI	GCATGC	2 cut(s)	4675, 5784
PaeR7I	CTCGAG	2 cut(s)	821, 2318
PagI	TCATGA	2 cut(s)	2077, 3508
PaqCI	CACCTGC	1 cut(s)	2923
PasI	CCCWGGG	1 cut(s)	4193
PciI	ACATGT	2 cut(s)	322, 5030
PciSI	GCTCTTC	1 cut(s)	3976
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 39
PctI	GAATGC	1 cut(s)	4235
PdmI	GAANNNNTTC	6 cut(s)	1374, 2252, 3120, 5137, 5677, 5833
PflMI	CCANNNNNTGG	3 cut(s)	1142, 2590, 4259
PfoI	TCCNGGA	1 cut(s)	3976
PinAI	ACCGGT	1 cut(s)	706
PmaCI	CACGTG	1 cut(s)	4085
PmlI	CACGTG	1 cut(s)	4085
Ppu21I	YACGTR	1 cut(s)	4085
PpuMI	RGGWCCY	2 cut(s)	3083, 3274
PscI	ACATGT	2 cut(s)	322, 5030
PshBI	ATTAAT	2 cut(s)	2013, 3572
PsiI	TTATAA	1 cut(s)	3173
Psp5II	RGGWCCY	2 cut(s)	3083, 3274
PspCI	CACGTG	1 cut(s)	4085
PspEI	GGTNACC	1 cut(s)	365
PspFI	CCCAGC	1 cut(s)	5425
PspOMI	GGGCCC	1 cut(s)	5325
PspPPI	RGGWCCY	2 cut(s)	3083, 3274
PspXI	VCTCGAGB	2 cut(s)	821, 2318
PstI	CTGCAG	7 cut(s)	1297, 2215, 3711, 5469, 5656, 5826, 5848
PstNI	CAGNNNCTG	7 cut(s)	1886, 2390, 2590, 3304, 4259, 4655, 5065
PsuI	RGATCY	8 cut(s)	605, 1196, 1831, 2975, 3772, 4273, 4408, 5350
PvuII	CAGCTG	4 cut(s)	1346, 3304, 4652, 5228
RseI	CAYNNNNRTG	2 cut(s)	327, 3825
SalI	GTCGAC	1 cut(s)	5721
SapI	GCTCTTC	1 cut(s)	3976
ScaI	AGTACT	3 cut(s)	3521, 3840, 4873
SduI	GDGCHC	2 cut(s)	1760, 5329
Sfr274I	CTCGAG	2 cut(s)	821, 2318
SfuI	TTCGAA	1 cut(s)	1165
SinI	GGWCC	9 cut(s)	343, 1028, 3083, 3224, 3274, 3559, 3974, 4483, 5602
SlaI	CTCGAG	2 cut(s)	821, 2318
SmiMI	CAYNNNNRTG	2 cut(s)	327, 3825
SmlI	CTYRAG	5 cut(s)	821, 1263, 2318, 3087, 4034
SmoI	CTYRAG	5 cut(s)	821, 1263, 2318, 3087, 4034
SphI	GCATGC	2 cut(s)	4675, 5784
SspI	AATATT	3 cut(s)	1651, 3382, 4521
SspMI	CTAG	8 cut(s)	1310, 2682, 3339, 3660, 4119, 4770, 5205, 5265
StyI	CCWWGG	6 cut(s)	599, 2335, 3079, 3601, 4351, 5264
TaiI	ACGT	9 cut(s)	1133, 1907, 2177, 3311, 4087, 4635, 4640, 5281, 5304
TaqII	GACCGA	1 cut(s)	2626
TatI	WGTACW	7 cut(s)	325, 1815, 3433, 3519, 3838, 3885, 4871
TauI	GCSGC	5 cut(s)	7, 1778, 3856, 4671, 4821
TseFI	GTSAC	6 cut(s)	421, 683, 2462, 2539, 3410, 4633
Tsp45I	GTSAC	6 cut(s)	421, 683, 2462, 2539, 3410, 4633
TspGWI	ACGGA	5 cut(s)	28, 1244, 2059, 3814, 4475
Van91I	CCANNNNNTGG	3 cut(s)	1142, 2590, 4259
VpaK11BI	GGWCC	9 cut(s)	343, 1028, 3083, 3224, 3274, 3559, 3974, 4483, 5602
VspI	ATTAAT	2 cut(s)	2013, 3572
XagI	CCTNNNNNAGG	2 cut(s)	344, 2680
XapI	RAATTY	6 cut(s)	132, 1034, 1150, 1676, 2186, 5133
XbaI	TCTAGA	2 cut(s)	3659, 4118
XceI	RCATGY	9 cut(s)	326, 1575, 1880, 4350, 4675, 4801, 5034, 5095, 5784
XcmI	CCANNNNNNNNNTGG	2 cut(s)	442, 695
XhoI	CTCGAG	2 cut(s)	821, 2318
XmaJI	CCTAGG	1 cut(s)	5264
XmiI	GTMKAC	5 cut(s)	659, 735, 872, 2946, 5722
XmnI	GAANNNNTTC	6 cut(s)	1374, 2252, 3120, 5137, 5677, 5833
XspI	CTAG	8 cut(s)	1310, 2682, 3339, 3660, 4119, 4770, 5205, 5265
ZraI	GACGTC	1 cut(s)	1131
ZrmI	AGTACT	3 cut(s)	3521, 3840, 4873
Zsp2I	ATGCAT	1 cut(s)	4235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.