Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0004063

Physical Location & Seq

Genomic Coordinates Reverse (-)

45652475 .. 45663473

Gene Structure 10999 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00424540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5193 bp

ATGCCGCCTTCCGACGGCTTCAGGGTCCGGATCGTCTCCGTCGACTTCTACATGTCGCCGCCCATCCCTGGACTCGATATCTCCTACAGTAGCTTCCACGGTGGGAAGGTGAATGAGGTACCTGTAATCAGAATTTACGGCTCTACTCCGGCCGGTCAGAAAACTTGTTTGCACATTCACAGAGCATTACCATACTTATTTGTGCCGTGCAAAGATATACCGATTCAACCACATCAGAAAGGTGATTCATACACATATGATATATCTCTTGCTTTAGAGAAAGCTTTGAAGCTCAAAAGCAATGCTGGTTCAAAGCGACAACATGTACATGGTTGCAGCCTTGTCCAGGCAAGGAAGCTTTACGGTTATCATTCATCAGAGGAGGTGTTTGTGAAGATATACCTCTATTATCCACATGATGTGACTCGTGCAGCCAATCTTCTTCTGGGAGGTGCAGTTATTGATAAATGCTTACAGCCCCACGAATCACACATTCCCTTCATTCTCCAATTTATGATTGACTACAACTTGTATGGGATGGGTCATCTGCATCTATCAAAACTGAAGTTTCGTCATCCAATGCCAGATGCTTTTACCCCAAGGAGATCTTTTTACAATGGACTGCCCGGTCAGGACATAGATAATTTAACTGGGATGTCTACAGATGTCCAGGCAGATATTAGTGACCATCTGCTTTTTGGTTCACCGGTTTGGATATCGTCCACAATTCCAGTAGACTGGACATGGCTTTCTCCTGATGATGTTGAGGCTTCATCGAATAGAGATGTTAACTGCACTAAACGACAAAGTATTTGTGAACTGGAGGGGGATGCTACTGTAGATGAGATACTCAATCAGCAGTTCAAAATGTATACATCCCTCTCGCAAACTCGTTCTGACGTGAAAATGGTTCAATCACTTATACCAATTTGGGAGGAGTTTGAGAGAAACGGGATACACGAGGCAGCAATATCTTCTAATCCTGCTAAACCACTTCCAGAAGATGTTTTGAAAACTTTATCACTTGGTCCAGAATTTAATGAGAAACTTGTCAAATTGCATTTTGATACAGAAAACTCTTTGCCTTTAACTCAATTAAAGAAAGATGTAAAGTCTATGCACCAAATGACCTCTCCAAAAGATGGAGTAAATTCTGTTGAACTTCGAACTGATAATCTCAGTTATGGAGAGATGATGGGATCTCTGTCGACACAAGGTTCTACTGACGAGACGGATGAAAAACTGAATAAAGAACTGCCAGCATCTCCAGCGATTCAGACATTAAGTATGAAGGCTGCAGATAATGAAGCACTAGCTCTGCTTAGATGGCTTGCCACTTCTCAGGCAGCTGATGATTTGAACTCTGATGATGAACTTGTTCAGGAGACAATACTGAGTCCTTTGTTGCCTGCAACAACTATTGACAAAGTTCTGGAGCAAGCTAATATGGATTATGAGAGTGAATCCCAAAAGGAATGTCAGGACATCCTTGATTCAGTTGGCGACTTTGAGGGATCAAAGGAAAAAGATTCGTATCCCACTGATCGGAACTGTTCTTCCAAAAGTGAACCTGCATGTATGATCCCCCAGGTGAATGGTACTGGGGATGATGTTCTCTCAACTCCATCTTCTTGTGCTGGATCAATCGAAAATATTTCTGAAGTAGAGATGAAGAATGAATTTAGGACTCCTGAACCCCAGGTTACGAAAGATGGTCTAAGCTCCAATCATAAACACAAAAGGAAAAAATTATTATGGGGCTCTTTACCTTTGTCTGCGGCCCAAAAACTGAAGACTGCGAGTGAACCTGTCAGTTTGTACAAGGAAACTAAGGATCCTGTTGGTACAAGTTCCTCCTCTGAAAGTGAAGTGAGAAAACATGCAGGCACTGATGCTTGTGATAGTACGTTGGGAAGATGCTCTCTGCGGGACTTGATGAGGAGAAAGCGATCTTATCGAATTGAGCCACCTGAAAAAGAAGATACACTTTATCAAATGTTGCATTTTGACGAAATTAATGGGAAGTCTCGTGAGTCTTTAGCTGACAGGCTATCATTAGCTCACCAACACTCAGTAAGTCATGAGGCTTATGATGTTAAAACAACCAATTCTGGCTGTTCCTTGTATGGTAAACTTCCACTTTTGTCTGGCAGTGTCAACTCTTTAAATGCAAGTATGTTAGATGGAAAATTTGACATGAATATCAAAATTGCTGCGGAAGTTGGCACTGGAGCAATTGCTCAACATGGGAACATCTTCACAGGCTCTTCTGAAGCTCCACCTGTGAATACAGGATTCTGGCAATTAAAGCCTGACAAGCTTGAGTCTGAAGCTTCCCTTGGCCGTTGTGAAACTTTCAATAAATCTAATGCTCAGACTTCCAAGTCAGGTGCTGAGGAAAACTTTTTTGTTGATGAGAGTCTTCATAGTTCTGCTGCTGACCCTGAAGTTCCTTGTGGAGATGGTCACACTGGAAGGGGCATTGATGGCTTATCTTCCAGAAGTGGAGTATCCGAGATACCTTTTGAGATTGGCGCAAATGTCACAACTGACAAAACTGCTTTAAACAATGAAAGGTGTGGTCGCCAGAAACTGGGGAGAGATTCCTTTTCATCTGGGCTTAGTAACTTGTTGCCCTCGGTCCTCCAGTCTGATGATAAGGACTTAATTGGCATGACCTTCGCTAGGAAACCCCCAATTGCAGACCGGAAGGATGGGGGATTTCTGAGTTCTTCATATTTACAAGAGGACGGAGCTTTGGATGATATTCTCCCATTTTTTGTGAGAGACTGCCAAAACGAGACAGACATCGAGAACAATTGTGTTAGAAATAGTGAGTCTCATTCTTATCAAGAATCTGCCTTAGGTGTCCCTACTCACTATCAAGCTGATGGCTCTTGTTTGTATCTGTTAACACCTGCAATTTCACCTCCTTCTGCCAAAAATGTATACCGCTGGCTGTCAAGAGGCAACAAAGGATCTTCTGCGAGTCTGACTGGTTCACAGAATTCTTTGCCTAATGATTTGGACGAGCCTTCACTCAAATATGGTTCTGTATGTGATGTGCAACCTAATCTACCCCAAGGACCTCAAGTAGATCATAGAAATCTTGAAGCAGAAAGGATTCCTTTTTGTAATGACATGACAGAAGTATTGGAAAGAGAAGCCGATGTTATAAGAGTTAAAACATACCCTGACCGCTCACAAGATAGTTCTCAGATTTCAGGTCCAGATGGGAGATCAAAATCCACTCCGCTTAGTCAAACTGGATTTCGAGACCCGGCAAGTGTTGGCGTTGGCCAGCAGCTGACGTTGTTAAGTATAGAGGTTCAAGCAGAATCTAGGGGTGATCTTCGACCCGATCCTCAGTTTGATGCTATCAATATTGTATTGCTTGCTATTCAGAATGATTGTGACTCCATCGTTGAAGTTCATGTACTTATGCGCAGCGATGCTGAAAATTGTCAGAGGAGTCATGACGGGATTTCTGACTGCAAGCTGTTTGTGTTTCATGAAGAGAAGTACTTATTTCATCATTTTATCAAGTTTGTATGCTCGTTGGACCCTGATATATTAATGGGTTGGGATATTCAAGGTGGTTCCCTTGGTTTTTTGGCTGAAAGGGCTTCACATCTTGGTATAGGCTTACTCAACAAGATATCTAGAACACCATCTGAAGCCAAGATGGTGGCTGAGGATTCAGAAGTTCCGGAGAAAGTCTTACAAGATAACCTGGCTGCTGACTCAATCATTGCTGATTCAGTTGTATTAGAAGATCCAATAATTGAGGATGAATGGGGACGGACTCATTCCAGCGGTGTCCATGTTGGTGGTAGAATTGTACTTAATGTCTGGCGGCTGATGCGCAGTGAAGTGAAGCTTAATATGTACACAGTTGAAGCTGTTTCTGAAGTTGTGTTGAGGCGGAAAGTCCCATATATTCCTAATAAACTACTCACAAAATGGTTTTTAAGTGGTCCTGGAAGAGCAAGATACCGGTGTATCAAATATGTGAAGGAGAGAGCAGAATTAAACCTTGAGATGATGAATCAATTAGATATGATAAATCGAACATCAGAACTTGCACGTGTCTTTGGCATTGACTTTTTTTCTGTTCTCTCTAGAGGCTCCCAATACCGTGTTGAATCTATGGTTCTGAGATTGGCACATACACAAAACTATCTTGCCATTTCCCCTGGGAAACAACAGGTAGCTTCGCAGCCAGCAATGGAATGCATCCCTCTTGTAATGGAACCAGAATCTGGTTTTTATGCAGATCCAGTAGTTGTTTTGGATTTTCAGTCTCTTTATCCATCAATGATAATTGCATACAACCTTTGCTATTCTACATGCCTTGGAAAAGTTGCGCCTTCAGAGCCAAATACATTTGGTGTCAGTTCATTTTCACCAGATCCACATGATCTGCATGATTTAAAAGATCAAATACTGCTTACTCCAAATGGTGTTATGTGTGTGCCAGCAAAGGTCCGTAAAGGCGTACTGCCACGCTTATTGGACGAAATATTGTCAACCAGAATAATGGTTAAACAAGCAATGAAAAAATTGTCTCCATCACAGAAAGTTCTTCACAGGATATTCAATGCAAGACAGCTTGCACTAAAGCTGATAGCAAATGTGACGTATGGTTATACAGCTGCTGGATTTAGTGGCCGCATGCCTTGTGCAGAGATTGCAGACAGTATTGTTCAGTGTGGCCGTAGCACACTTGAAAAGGCTATTTCATATGTAAATGCACATGACAGGTGGAAGGCCAGAGTTATTTATGGCGACACAGACAGCATGTTTGTCCTTCTAAAAGGCCGCACTAGGGAAGAGTCTTTTCAGATTGGACAGGAGATTGCATCTGAAATCAGTGCTATGAGTCCAACTCCTGTTGCTTTGAAGATGGAAAAAGTTTATCAGCCATGCTTCCTCCTTACCAAGAAGCGATATGTTGGTTACAGTTATGAAAGCCCTGGACAGAGTGAACCTATTTTTGATGCTAAAGGCATTGAGACAGTTCGCAGGGATACATGTGGGGCTGTTGCCAAGACAATGGAGCAGTCTCTGAGACTTTTTTTGAACATCAAGACATGCCTGAGGTCAAAGCATACTTACAGCGCCAGTGGAAGCGAATTCTTTCTGGAAGAGTGTCCCTTCAAGATTTTGTATTTGCAAAAGAGGTTCGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000731 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010225 GO:0016035 GO:0018130 GO:0019438 GO:0019985 GO:0032991 GO:0033554 GO:0034641 GO:0034644 GO:0034645 GO:0034654 GO:0042276 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0071214 GO:0071478 GO:0071482 GO:0071494 GO:0071704 GO:0071897 GO:0090304 GO:0104004 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko01100 ko01524 ko03460 map01100 map01524 map03460

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 RVT_2 zf-C4pol zf-RanBP

Protein Analysis

1730

Amino Acids

191.79

Weight (kDa)

5.61

Isoelectric Point (pI)

49.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
REV3_N	PF24065	7 - 60	1.7e-17	DNA polymerase zeta catalytic subunit, N-terminal
POL3_N	PF24055	62 - 141	2.7e-22	DNA polymerase delta catalytic subunit-like, N-terminal domain
DNA_pol_B_exo1	PF03104	1101 - 1227	9.4e-09	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	1369 - 1701	9.4e-85	DNA polymerase family B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004063)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500
fragaria_vesca	FvH4_6g06120 FvH4_6g06120 FvH4_6g06120 FvH4_6g06120 FvH4_6g06120
malus_domestica	MD05G1053400.v1.1 MD05G1053600.v1.1 MD05G1053700.v1.1 MD12G1217900.v1.1
prunus_persica	Prupe.6G324000_v2.0.a1 Prupe.6G324000_v2.0.a1 Prupe.6G324000_v2.0.a1
pyrus_communis	pycom05g04420 pycom12g20340
rosa_chinensis	RchiOBHm_Chr3g0455131
rosa_laevigata	RLG00000025385
rosa_multiflora	Rmu_co8200746.1_g000001 Rmu_sc0041669.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424540
rosa_rugosa	Rorug01G0381700 Rorug03G0004000
rosa_samantha	Rh3AG064900 Rh3BG066900 Rh3CG065300 Rh3DG066800
rosa_wichuraiana	Rw3G005080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3176
AarI	CACCTGC	1 cut(s)	2926
AasI	GACNNNNNNGTC	2 cut(s)	627, 2383
Acc16I	TGCGCA	2 cut(s)	3446, 3866
Acc36I	ACCTGC	2 cut(s)	1579, 2926
Acc65I	GGTACC	1 cut(s)	118
AccB1I	GGYRCC	1 cut(s)	118
AccB7I	CCANNNNNTGG	3 cut(s)	1142, 2593, 4262
AccBSI	CCGCTC	1 cut(s)	3201
AccI	GTMKAC	6 cut(s)	42, 659, 735, 872, 1208, 2949
AccIII	TCCGGA	2 cut(s)	27, 3709
AcoI	YGGCCR	5 cut(s)	150, 2341, 3298, 4669, 4714
AcsI	RAATTY	7 cut(s)	132, 1034, 1150, 1679, 2189, 3007, 5135
AcvI	CACGTG	1 cut(s)	4088
AfiI	CCNNNNNNNGG	9 cut(s)	14, 68, 346, 1142, 1545, 2593, 2685, 4262, 4828
AflIII	ACRYGT	4 cut(s)	51, 322, 4087, 5033
AgeI	ACCGGT	2 cut(s)	706, 3996
AjiI	CACGTC	1 cut(s)	901
AjnI	CCWGG	9 cut(s)	67, 345, 669, 1587, 1698, 3732, 3979, 4195, 4975
AjuI	GAANNNNNNNTTGG	2 cut(s)	2322, 2354
AloI	GAACNNNNNNTCC	2 cut(s)	1541, 1573
AlwNI	CAGNNNCTG	7 cut(s)	1889, 2393, 2593, 3307, 4262, 4658, 5068
Aor13HI	TCCGGA	2 cut(s)	27, 3709
AoxI	GGCC	8 cut(s)	150, 1779, 2341, 3298, 4669, 4714, 4770, 4819
ApoI	RAATTY	7 cut(s)	132, 1034, 1150, 1679, 2189, 3007, 5135
ArsI	GACNNNNNNTTYG	6 cut(s)	795, 827, 1462, 1494, 4787, 4819
AseI	ATTAAT	2 cut(s)	2016, 3575
AsiGI	ACCGGT	2 cut(s)	706, 3996
Asp700I	GAANNNNTTC	4 cut(s)	1377, 2255, 3123, 5139
Asp718I	GGTACC	1 cut(s)	118
AspLEI	GCGC	5 cut(s)	2537, 3447, 3867, 4369, 5123
AspS9I	GGNCC	9 cut(s)	25, 1028, 1780, 2641, 3086, 3227, 3562, 3977, 4486
AsuC2I	CCSGG	2 cut(s)	627, 3281
AsuHPI	GGTGA	8 cut(s)	121, 254, 696, 1603, 2054, 2919, 3359, 4398
AsuII	TTCGAA	1 cut(s)	1165
AvaII	GGWCC	8 cut(s)	25, 1028, 2641, 3086, 3227, 3562, 3977, 4486
AxyI	CCTNAGG	2 cut(s)	2863, 5099
BaeI	ACNNNNGTAYC	4 cut(s)	3985, 3985, 4018, 4018
BalI	TGGCCA	1 cut(s)	3300
BamHI	GGATCC	1 cut(s)	1834
BanI	GGYRCC	1 cut(s)	118
BanII	GRGCYC	1 cut(s)	1763
BarI	GAAGNNNNNNTAC	2 cut(s)	3420, 3452
BauI	CACGAG	3 cut(s)	426, 959, 2028
BbrPI	CACGTG	1 cut(s)	4088
BbsI	GAAGAC	2 cut(s)	1799, 2414
BbvCI	CCTCAGC	2 cut(s)	2394, 3693
BceAI	ACGGC	5 cut(s)	31, 154, 190, 2328, 4701
BcgI	CGANNNNNNTGC	2 cut(s)	2662, 2696
BciT130I	CCWGG	9 cut(s)	69, 347, 671, 1589, 1700, 3734, 3981, 4197, 4977
BciVI	GTATCC	4 cut(s)	948, 1545, 2521, 5023
BcnI	CCSGG	2 cut(s)	627, 3281
BfaI	CTAG	7 cut(s)	1313, 2685, 3342, 3663, 4122, 4827, 5191
BfmI	CTRYAG	4 cut(s)	85, 660, 837, 1296
BfoI	RGCGCY	1 cut(s)	5124
BfuAI	ACCTGC	2 cut(s)	1579, 2926
BfuI	GTATCC	4 cut(s)	948, 1545, 2521, 5023
BglII	AGATCT	1 cut(s)	605
BmcAI	AGTACT	1 cut(s)	3524
Bme18I	GGWCC	8 cut(s)	25, 1028, 2641, 3086, 3227, 3562, 3977, 4486
BmgBI	CACGTC	1 cut(s)	901
BmgT120I	GGNCC	9 cut(s)	25, 1028, 1780, 2641, 3086, 3227, 3562, 3977, 4486
BmiI	GGNNCC	7 cut(s)	26, 120, 1836, 3564, 3601, 4129, 4254
BmrI	ACTGGG	3 cut(s)	660, 1611, 2603
BmuI	ACTGGG	3 cut(s)	660, 1611, 2603
BpiI	GAAGAC	2 cut(s)	1799, 2414
BplI	GAGNNNNNCTC	2 cut(s)	4873, 4905
BpmI	CTGGAG	5 cut(s)	842, 1251, 1454, 2250, 2630
Bpu10I	CCTNAGC	2 cut(s)	2394, 3693
Bpu14I	TTCGAA	1 cut(s)	1165
BpuEI	CTTGAG	3 cut(s)	2342, 3075, 4058
BpuMI	CCSGG	2 cut(s)	627, 3281
BsaAI	YACGTR	1 cut(s)	4088
BsaBI	GATNNNNATC	2 cut(s)	1533, 3244
BsaI	GGTCTC	1 cut(s)	3270
BsaWI	WCCGGW	5 cut(s)	27, 706, 2706, 3709, 3996
BsaXI	ACNNNNNCTCC	2 cut(s)	441, 471
Bsc4I	CCNNNNNNNGG	9 cut(s)	14, 68, 346, 1142, 1545, 2593, 2685, 4262, 4828
Bse118I	RCCGGY	3 cut(s)	152, 706, 3996
Bse21I	CCTNAGG	2 cut(s)	2863, 5099
Bse3DI	GCAATG	4 cut(s)	307, 3750, 4233, 4560
Bse8I	GATNNNNATC	2 cut(s)	1533, 3244
BseAI	TCCGGA	2 cut(s)	27, 3709
BseBI	CCWGG	9 cut(s)	69, 347, 671, 1589, 1700, 3734, 3981, 4197, 4977
BseJI	GATNNNNATC	2 cut(s)	1533, 3244
BseLI	CCNNNNNNNGG	9 cut(s)	14, 68, 346, 1142, 1545, 2593, 2685, 4262, 4828
BseMI	GCAATG	4 cut(s)	307, 3750, 4233, 4560
BseRI	GAGGAG	5 cut(s)	395, 950, 1846, 1954, 3484
BseX3I	CGGCCG	1 cut(s)	150
BsgI	GTGCAG	4 cut(s)	450, 474, 778, 4704
Bsh1285I	CGRYCG	1 cut(s)	153
BshFI	GGCC	8 cut(s)	152, 1781, 2343, 3300, 4671, 4716, 4772, 4821
BshNI	GGYRCC	1 cut(s)	118
BshTI	ACCGGT	2 cut(s)	706, 3996
BsiEI	CGRYCG	1 cut(s)	153
BsiSI	CCGG	9 cut(s)	28, 149, 153, 627, 707, 2707, 3281, 3710, 3997
BslFI	GGGAC	5 cut(s)	1943, 2855, 3813, 3917, 5139
BslI	CCNNNNNNNGG	9 cut(s)	14, 68, 346, 1142, 1545, 2593, 2685, 4262, 4828
BsmBI	CGTCTC	2 cut(s)	40, 1223
BsmFI	GGGAC	5 cut(s)	1943, 2855, 3813, 3917, 5139
BsmI	GAATGC	1 cut(s)	4238
BsnI	GGCC	8 cut(s)	152, 1781, 2343, 3300, 4671, 4716, 4772, 4821
Bso31I	GGTCTC	1 cut(s)	3270
Bsp119I	TTCGAA	1 cut(s)	1165
Bsp1286I	GDGCHC	1 cut(s)	1763
Bsp13I	TCCGGA	2 cut(s)	27, 3709
Bsp1407I	TGTACA	3 cut(s)	325, 1818, 3888
BspANI	GGCC	8 cut(s)	152, 1781, 2343, 3300, 4671, 4716, 4772, 4821
BspEI	TCCGGA	2 cut(s)	27, 3709
BspHI	TCATGA	3 cut(s)	2080, 3475, 3511
BspLI	GGNNCC	7 cut(s)	26, 120, 1836, 3564, 3601, 4129, 4254
BspMAI	CTGCAG	1 cut(s)	1300
BspMI	ACCTGC	2 cut(s)	1579, 2926
BspQI	GCTCTTC	2 cut(s)	2272, 3979
BspT104I	TTCGAA	1 cut(s)	1165
BspT107I	GGYRCC	1 cut(s)	118
BspTNI	GGTCTC	1 cut(s)	3270
BsrBI	CCGCTC	1 cut(s)	3201
BsrDI	GCAATG	4 cut(s)	307, 3750, 4233, 4560
BsrFI	RCCGGY	3 cut(s)	152, 706, 3996
BsrGI	TGTACA	3 cut(s)	325, 1818, 3888
BssAI	RCCGGY	3 cut(s)	152, 706, 3996
BssNAI	GTATAC	2 cut(s)	873, 2950
BssSI	CACGAG	3 cut(s)	426, 959, 2028
BssT1I	CCWWGG	5 cut(s)	599, 2338, 3082, 3604, 4354
Bst1107I	GTATAC	2 cut(s)	873, 2950
Bst2BI	CACGAG	3 cut(s)	426, 959, 2028
Bst2UI	CCWGG	9 cut(s)	69, 347, 671, 1589, 1700, 3734, 3981, 4197, 4977
Bst6I	CTCTTC	5 cut(s)	2272, 3510, 3979, 4827, 5142
BstAPI	GCANNNNNTGC	1 cut(s)	4691
BstAUI	TGTACA	3 cut(s)	325, 1818, 3888
BstBAI	YACGTR	1 cut(s)	4088
BstBI	TTCGAA	1 cut(s)	1165
BstDSI	CCRYGG	1 cut(s)	97
BstENI	CCTNNNNNAGG	2 cut(s)	344, 2683
BstH2I	RGCGCY	1 cut(s)	5124
BstHHI	GCGC	5 cut(s)	2537, 3447, 3867, 4369, 5123
BstMCI	CGRYCG	1 cut(s)	153
BstMWI	GCNNNNNNNGC	9 cut(s)	1268, 1327, 2308, 2317, 2487, 3623, 3862, 4375, 4691
BstNI	CCWGG	9 cut(s)	69, 347, 671, 1589, 1700, 3734, 3981, 4197, 4977
BstNSI	RCATGY	9 cut(s)	55, 326, 1578, 1883, 4353, 4678, 4804, 5037, 5097
BstSFI	CTRYAG	4 cut(s)	85, 660, 837, 1296
BstV2I	GAAGAC	2 cut(s)	1799, 2414
BstX2I	RGATCY	7 cut(s)	605, 1199, 1834, 2978, 3775, 4276, 4411
BstXI	CCANNNNNNTGG	6 cut(s)	738, 1595, 3688, 3830, 4540, 5056
BstYI	RGATCY	7 cut(s)	605, 1199, 1834, 2978, 3775, 4276, 4411
BstZ17I	GTATAC	2 cut(s)	873, 2950
BstZI	CGGCCG	1 cut(s)	150
Bsu36I	CCTNAGG	2 cut(s)	2863, 5099
BsuI	GTATCC	4 cut(s)	948, 1545, 2521, 5023
BsuRI	GGCC	8 cut(s)	152, 1781, 2343, 3300, 4671, 4716, 4772, 4821
BtgI	CCRYGG	1 cut(s)	97
BtgZI	GCGATG	1 cut(s)	3465
BtrI	CACGTC	1 cut(s)	901
BtsI	GCAGTG	2 cut(s)	2158, 3874
BtsIMutI	CAGTG	9 cut(s)	1539, 1887, 2158, 2226, 2469, 3874, 4715, 4879, 5131
BveI	ACCTGC	2 cut(s)	1579, 2926
CaiI	CAGNNNCTG	7 cut(s)	1889, 2393, 2593, 3307, 4262, 4658, 5068
CciI	TCATGA	3 cut(s)	2080, 3475, 3511
CfoI	GCGC	5 cut(s)	2537, 3447, 3867, 4369, 5123
Cfr10I	RCCGGY	3 cut(s)	152, 706, 3996
Cfr13I	GGNCC	9 cut(s)	25, 1028, 1780, 2641, 3086, 3227, 3562, 3977, 4486
CspAI	ACCGGT	2 cut(s)	706, 3996
CspCI	CAANNNNNGTGG	2 cut(s)	4494, 4529
DraI	TTTAAA	3 cut(s)	2166, 2565, 4434
DrdI	GACNNNNNNGTC	2 cut(s)	627, 2383
DseDI	GACNNNNNNGTC	2 cut(s)	627, 2383
EaeI	YGGCCR	5 cut(s)	150, 2341, 3298, 4669, 4714
EagI	CGGCCG	1 cut(s)	150
Eam1104I	CTCTTC	5 cut(s)	2272, 3510, 3979, 4827, 5142
EarI	CTCTTC	5 cut(s)	2272, 3510, 3979, 4827, 5142
EciI	GGCGGA	1 cut(s)	3940
EclXI	CGGCCG	1 cut(s)	150
Eco130I	CCWWGG	5 cut(s)	599, 2338, 3082, 3604, 4354
Eco24I	GRGCYC	1 cut(s)	1763
Eco31I	GGTCTC	1 cut(s)	3270
Eco32I	GATATC	3 cut(s)	79, 717, 3660
Eco47I	GGWCC	8 cut(s)	25, 1028, 2641, 3086, 3227, 3562, 3977, 4486
Eco52I	CGGCCG	1 cut(s)	150
Eco72I	CACGTG	1 cut(s)	4088
Eco81I	CCTNAGG	2 cut(s)	2863, 5099
EcoNI	CCTNNNNNAGG	2 cut(s)	344, 2683
EcoO109I	RGGNCCY	1 cut(s)	3086
EcoRI	GAATTC	2 cut(s)	3007, 5135
EcoRII	CCWGG	9 cut(s)	67, 345, 669, 1587, 1698, 3732, 3979, 4195, 4975
EcoRV	GATATC	3 cut(s)	79, 717, 3660
EcoT14I	CCWWGG	5 cut(s)	599, 2338, 3082, 3604, 4354
EcoT22I	ATGCAT	1 cut(s)	4238
EcoT38I	GRGCYC	1 cut(s)	1763
ErhI	CCWWGG	5 cut(s)	599, 2338, 3082, 3604, 4354
Esp3I	CGTCTC	2 cut(s)	40, 1223
FaqI	GGGAC	5 cut(s)	1943, 2855, 3813, 3917, 5139
FauI	CCCGC	1 cut(s)	1920
FauNDI	CATATG	2 cut(s)	256, 4744
FblI	GTMKAC	6 cut(s)	42, 659, 735, 872, 1208, 2949
FriOI	GRGCYC	1 cut(s)	1763
FspBI	CTAG	7 cut(s)	1313, 2685, 3342, 3663, 4122, 4827, 5191
FspI	TGCGCA	2 cut(s)	3446, 3866
GlaI	GCGC	5 cut(s)	2536, 3446, 3866, 4368, 5122
GsuI	CTGGAG	5 cut(s)	842, 1251, 1454, 2250, 2630
HaeII	RGCGCY	1 cut(s)	5124
HaeIII	GGCC	8 cut(s)	152, 1781, 2343, 3300, 4671, 4716, 4772, 4821
HapII	CCGG	9 cut(s)	28, 149, 153, 627, 707, 2707, 3281, 3710, 3997
HhaI	GCGC	5 cut(s)	2537, 3447, 3867, 4369, 5123
Hin6I	GCGC	5 cut(s)	2535, 3445, 3865, 4367, 5121
HinP1I	GCGC	5 cut(s)	2535, 3445, 3865, 4367, 5121
HincII	GTYRAC	6 cut(s)	43, 790, 1209, 2158, 2913, 4530
HindII	GTYRAC	6 cut(s)	43, 790, 1209, 2158, 2913, 4530
HindIII	AAGCTT	5 cut(s)	282, 356, 2318, 2331, 3878
HpaI	GTTAAC	2 cut(s)	790, 2913
HpaII	CCGG	9 cut(s)	28, 149, 153, 627, 707, 2707, 3281, 3710, 3997
HphI	GGTGA	8 cut(s)	121, 254, 696, 1603, 2054, 2919, 3359, 4398
Hpy99I	CGWCG	2 cut(s)	17, 44
HpyCH4IV	ACGT	5 cut(s)	900, 1907, 3311, 4087, 4640
HpyF10VI	GCNNNNNNNGC	9 cut(s)	1268, 1327, 2308, 2317, 2487, 3623, 3862, 4375, 4691
HpySE526I	ACGT	5 cut(s)	900, 1907, 3311, 4087, 4640
HspAI	GCGC	5 cut(s)	2535, 3445, 3865, 4367, 5121
Kpn2I	TCCGGA	2 cut(s)	27, 3709
KpnI	GGTACC	1 cut(s)	122
KspAI	GTTAAC	2 cut(s)	790, 2913
LguI	GCTCTTC	2 cut(s)	2272, 3979
LmnI	GCTCC	7 cut(s)	1435, 1727, 2231, 2281, 2753, 4133, 5059
MaeI	CTAG	7 cut(s)	1313, 2685, 3342, 3663, 4122, 4827, 5191
MaeII	ACGT	5 cut(s)	900, 1907, 3311, 4087, 4640
MaeIII	GTNAC	9 cut(s)	421, 683, 1702, 2465, 2542, 2624, 3413, 4636, 4958
MbiI	CCGCTC	1 cut(s)	3201
MfeI	CAATTG	3 cut(s)	2235, 2697, 2818
MflI	RGATCY	7 cut(s)	605, 1199, 1834, 2978, 3775, 4276, 4411
MhlI	GDGCHC	1 cut(s)	1763
MlsI	TGGCCA	1 cut(s)	3300
MluNI	TGGCCA	1 cut(s)	3300
MmeI	TCCRAC	3 cut(s)	36, 3540, 4910
Mox20I	TGGCCA	1 cut(s)	3300
Mph1103I	ATGCAT	1 cut(s)	4238
MroI	TCCGGA	2 cut(s)	27, 3709
MroXI	GAANNNNTTC	4 cut(s)	1377, 2255, 3123, 5139
MscI	TGGCCA	1 cut(s)	3300
MslI	CAYNNNNRTG	2 cut(s)	327, 3828
Msp20I	TGGCCA	1 cut(s)	3300
MspA1I	CMGCKG	5 cut(s)	1349, 2955, 3307, 3816, 4655
MspI	CCGG	9 cut(s)	28, 149, 153, 627, 707, 2707, 3281, 3710, 3997
MunI	CAATTG	3 cut(s)	2235, 2697, 2818
Mva1269I	GAATGC	1 cut(s)	4238
MvaI	CCWGG	9 cut(s)	69, 347, 671, 1589, 1700, 3734, 3981, 4197, 4977
MwoI	GCNNNNNNNGC	9 cut(s)	1268, 1327, 2308, 2317, 2487, 3623, 3862, 4375, 4691
NciI	CCSGG	2 cut(s)	627, 3281
NdeI	CATATG	2 cut(s)	256, 4744
NlaIV	GGNNCC	7 cut(s)	26, 120, 1836, 3564, 3601, 4129, 4254
NmuCI	GTSAC	6 cut(s)	421, 683, 2465, 2542, 3413, 4636
NsbI	TGCGCA	2 cut(s)	3446, 3866
NsiI	ATGCAT	1 cut(s)	4238
NspI	RCATGY	9 cut(s)	55, 326, 1578, 1883, 4353, 4678, 4804, 5037, 5097
NspV	TTCGAA	1 cut(s)	1165
PaeI	GCATGC	1 cut(s)	4678
PagI	TCATGA	3 cut(s)	2080, 3475, 3511
PaqCI	CACCTGC	1 cut(s)	2926
PasI	CCCWGGG	1 cut(s)	4196
PciI	ACATGT	3 cut(s)	51, 322, 5033
PciSI	GCTCTTC	2 cut(s)	2272, 3979
PcsI	WCGNNNNNNNCGW	2 cut(s)	39, 957
PctI	GAATGC	1 cut(s)	4238
PdmI	GAANNNNTTC	4 cut(s)	1377, 2255, 3123, 5139
PflMI	CCANNNNNTGG	3 cut(s)	1142, 2593, 4262
PfoI	TCCNGGA	1 cut(s)	3979
PinAI	ACCGGT	2 cut(s)	706, 3996
PmaCI	CACGTG	1 cut(s)	4088
PmlI	CACGTG	1 cut(s)	4088
Ppu21I	YACGTR	1 cut(s)	4088
PpuMI	RGGWCCY	1 cut(s)	3086
PscI	ACATGT	3 cut(s)	51, 322, 5033
PshBI	ATTAAT	2 cut(s)	2016, 3575
PsiI	TTATAA	1 cut(s)	3176
Psp5II	RGGWCCY	1 cut(s)	3086
Psp6I	CCWGG	9 cut(s)	67, 345, 669, 1587, 1698, 3732, 3979, 4195, 4975
PspCI	CACGTG	1 cut(s)	4088
PspGI	CCWGG	9 cut(s)	67, 345, 669, 1587, 1698, 3732, 3979, 4195, 4975
PspN4I	GGNNCC	7 cut(s)	26, 120, 1836, 3564, 3601, 4129, 4254
PspPI	GGNCC	9 cut(s)	25, 1028, 1780, 2641, 3086, 3227, 3562, 3977, 4486
PspPPI	RGGWCCY	1 cut(s)	3086
PstI	CTGCAG	1 cut(s)	1300
PstNI	CAGNNNCTG	7 cut(s)	1889, 2393, 2593, 3307, 4262, 4658, 5068
PsuI	RGATCY	7 cut(s)	605, 1199, 1834, 2978, 3775, 4276, 4411
PvuII	CAGCTG	3 cut(s)	1349, 3307, 4655
RseI	CAYNNNNRTG	2 cut(s)	327, 3828
SalI	GTCGAC	2 cut(s)	41, 1207
SapI	GCTCTTC	2 cut(s)	2272, 3979
Sau96I	GGNCC	9 cut(s)	25, 1028, 1780, 2641, 3086, 3227, 3562, 3977, 4486
ScaI	AGTACT	1 cut(s)	3524
SduI	GDGCHC	1 cut(s)	1763
SfcI	CTRYAG	4 cut(s)	85, 660, 837, 1296
SfuI	TTCGAA	1 cut(s)	1165
SinI	GGWCC	8 cut(s)	25, 1028, 2641, 3086, 3227, 3562, 3977, 4486
SmiMI	CAYNNNNRTG	2 cut(s)	327, 3828
SmlI	CTYRAG	3 cut(s)	2321, 3090, 4037
SmoI	CTYRAG	3 cut(s)	2321, 3090, 4037
SphI	GCATGC	1 cut(s)	4678
SspI	AATATT	3 cut(s)	1654, 3385, 4524
SspMI	CTAG	7 cut(s)	1313, 2685, 3342, 3663, 4122, 4827, 5191
StyI	CCWWGG	5 cut(s)	599, 2338, 3082, 3604, 4354
TaiI	ACGT	5 cut(s)	903, 1910, 3314, 4090, 4643
TaqII	GACCGA	1 cut(s)	2629
TatI	WGTACW	6 cut(s)	325, 1818, 3436, 3522, 3841, 3888
TauI	GCSGC	6 cut(s)	7, 61, 1781, 3859, 4674, 4824
TscAI	CASTG	9 cut(s)	1546, 1894, 2158, 2233, 2476, 3874, 4715, 4879, 5131
TseFI	GTSAC	6 cut(s)	421, 683, 2465, 2542, 3413, 4636
Tsp45I	GTSAC	6 cut(s)	421, 683, 2465, 2542, 3413, 4636
TspGWI	ACGGA	5 cut(s)	28, 1247, 2766, 3817, 4478
TspRI	CASTG	9 cut(s)	1546, 1894, 2158, 2233, 2476, 3874, 4715, 4879, 5131
Van91I	CCANNNNNTGG	3 cut(s)	1142, 2593, 4262
VpaK11BI	GGWCC	8 cut(s)	25, 1028, 2641, 3086, 3227, 3562, 3977, 4486
VspI	ATTAAT	2 cut(s)	2016, 3575
XagI	CCTNNNNNAGG	2 cut(s)	344, 2683
XapI	RAATTY	7 cut(s)	132, 1034, 1150, 1679, 2189, 3007, 5135
XbaI	TCTAGA	2 cut(s)	3662, 4121
XceI	RCATGY	9 cut(s)	55, 326, 1578, 1883, 4353, 4678, 4804, 5037, 5097
XcmI	CCANNNNNNNNNTGG	2 cut(s)	442, 695
XmiI	GTMKAC	6 cut(s)	42, 659, 735, 872, 1208, 2949
XmnI	GAANNNNTTC	4 cut(s)	1377, 2255, 3123, 5139
XspI	CTAG	7 cut(s)	1313, 2685, 3342, 3663, 4122, 4827, 5191
ZrmI	AGTACT	1 cut(s)	3524
Zsp2I	ATGCAT	1 cut(s)	4238

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.