Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0004063

Physical Location & Seq

Genomic Coordinates Forward (+)

4503776 .. 4514740

Gene Structure 10965 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG066800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5274 bp

ATGCCGCCTTCCGACAGCTTCAGCGTCCGGATCGTCTCCGTCGACTTCTACATGGCGCCGCCCATCCCTGGCTTCGATATCTCCTACAGTAGCTTCCACGGTGGGAAGGTGAATGAGGTACCTGTAATCAGAATTTACGGCTCTACTCCGGCCGGTCAGAAAGCTTGTCTGCACATTCACAGAGCATTACCATACCTATTTGTGCCGTGCAAAGATATACCGATTCAACCACATCAGAAAGGTGATTCATACACATATGATATATCTCTCGCATTAGAGAAAGCTTTGAAGCTCAAAAGCAGTGCTGGTTCAAAGCGACAACATGTACATGGTTGCAGCCTTGTCCAGGCAAGGAAGCTTTACGGTTACCATTCATCAGAGGAGGTGTTTGTGAAGATATACCTCTATTATCCACATGATGTGACTCGCGCCGCCAATCTTCTTCTGGGAGGTGCAGTGCTTGATAAATGCTTACAGCCCCACGAATCACACATTCCCTTCATTCTCCAATTTATGATTGACTACAACTTGTATGGGATGGGTCATCTGCATCTATCAAAACTGAAGTTTCGTCATCCAATGCCAGATGCTTTTACCCCTAGGAGATCTTTTTACAATGGACTGCCCGGTCAGGACATAGATAATTTAACTGGGATGTCTACAGATGTCCAGGCAGATATTAGTGACCATCTGCTTTTTGGTTCACCGGTTTGGATATCGTCCACAATTCCAGTAGACTGGACATGGCTTTCTTCTGGTGATGTTGAGGCTTCATCAAATAGAGATGCTAACTGCACTAAACGACAAAGTATTTGTGAACTCGAGGGGGATGCTACTGTAGATGAGATACTCAATCAGCAGTTCAAAATGTATACATCCCTCTCGCAAACTCGTTCTGACGTGAAAATGGTTCAATCACTTATACCAATTTGGGAGGAGTTTGAGAAAAATGGGATACACGAGGCAGCAATATCTTCTAATCCTGCTAAACCACTTCCAGAAGATGTTTTGAAAACTTTATCACTTGGTCCAGAATTTAATGAGAAACTTGTCAAATTGCATTTTGATACAGAAAACTCTTTGCCTTTAACTCAATTAAAGAAAGATGTAAAGTCTATGCACCAAATGCCCTCTCCAAAAGATGGAGGAAATTCTGTTGGACTTCGAACTGATAATCTCAATTATGGAGAGATGATGGGATCTCTGTTGACACAAGGTTCTACTGAAGAGACGGATGCAAAACTGAATAAAGAACTGCCAGCATCTCAAGCGATTCAGACATTAAGTATGAAGGCTGCAGATAATGAAGCACTAGCTCTGCTTAGATGGCTTGCCACTTCTCAGGCAGCTGATGATTTGAACTCCGATGATGAACTTGTTCAGGAGACAATACTGAGTCCTTTGTTGCCTGCAACAACTATTGACAAAGTTCTGGAGAAAGCTAATATGGATTATGAGAGTGAATCCCAAAAGGAATGTCAGGACATCCTTGATTCGGTTGGCTACTTTGAGGGATCAAAGGAAAAAGATTCTTATCCCACTGATCGGAACTGTTCTTCCAAAAGTGAACCTGCATGTATGATTCTCCAGGTGAATGGTACTGGGGATGATGTTCTCTCAACTCCATCTCCTTGTGCTGGATCAATCGAAAATATTTCTGAAGTAGAGATGAAGAATGAACTTAGGACTCCTGAACCCCAGGTTACGAAAGATGGTCTAAGCTCCAATCATAAACACAAAAGGAAAAAATTATTATGGGGCTCTTTACCTTTGTCTGCGGCCCAAAAGCTGAAGACTGAGAGTGAACCTGTCAGTTTGTACAAGGAAACTAAGGATCCTGTTGGTACAAGTGCCTCCTCTGAAAGTGAAGTGAGAAAACATGCAGGCACTGATGCTTGTGATAGTACGTTGGGAAGATGCTCTCTGCGGGACTTGATGAGGAGAAAGCGATCTTATCGAATTGAGCCACCTGAAAAAGAAGATACACTTTATCAAATGTTGCATTTTGACGAACTTAATGGGAAGTCTCGTGATTCTTTAGCTGACAGGCTATCATTAGCCCGACACTCAGTAAGTCATGAGGCTGATGATGTTAAAACAACCAATTCTGGCTGTTCCTTGTATGGTAAACTTCCACTTTTGTCTGGCAGTGTCAACTCTTTAAATGCAAGTACGTTAGATGGAAAATTTGACACGAATATCAAAATTGCTGCGGAAGTTGGCACTGGAGCAATTGCTCAACATGGGAACATCTTCACAGGCTCTTCTGAAGCTCCACCTGTGAATACAGGATTCTGGCAATTAAAGCCTGACAAGCTCGAGTCTGAAGCTTCCCTTGGCCGTTGTGAAACTTTCAACAAATCTAATGCTCAGACTTCCAAGTCAGGTGCTGAGGAAAACTTTTTTGTTGATGAGAGTCTTCATAGTTCTGCTGCTGACCCTGAAGTTTCTTGTGGAGATGGTCACACTGGAAGGGGCATTGATGGCTTATCTTCCAGAAGTGGAGTATCCGAGATACCTTTTGAGATTGGCACAAATGTCACAACTGACAAAACTGCTTTAAACAATGAAAGGTGTGGTAGCCAGAAACTGGGGAGAGATTCCTTTTTATCTGGGCTTAGTAACTTGTTGCCCTCGATCATCCAGTCTGATGATAAGGACTTAATTGGCATGACCTTCTCTAGGAAACCCCCAATTGCAGACTGGAAGGATGGGGGATTTCTGAGTTCTTCATATTTACAAGAGGAAGGAGCTTTGGATGATATTCTCCCATTTTTTGTGAGAGACTGCCAAAATGAGACAGACATCGAGAACAACTGTGTTAGAAATAGTGAGTCTCATTCTCATCAAGAATCTGCCTTAGGTGTCCCTACTCACTATCAAGCTGATGGCTCTTGTTTGTATCTGTTAACACCTGCAATTTCACCTCCTTCTGCCAAAAATGTATACCGCTGGCTGTCAAGAGGCGACAAAGGATCTTCTGCGAGTCTGACTGGTTCACAGAATTCTTTGCCTAATGATTTGAACGAGCCTTCACTCAAATATGGTTATGTATATGATGTGCAACCTAATCTACCCCAAGGACCTCAAGTAGATCATAGAAATCTTGAAGCAGAAAGGATTCCTTTTTGTAATGACATGACAGAAGTATTGGAAAGAGAAGCCGATGTTATAAGAGTTAAAACATACCCTGACCGCTCACAAGATAGTTCTCAGATTTCAGGTCCAGATGGGAGATCAAAATCCACTCCGCTTAGTCAAACTGGATTTCGAGACCCGGCAAGTGTTGGCATTGGCCAGCAGCTGACGTTGTTAAGTATAGAGGTTCAAGCAGAATCTAGAGGTGATCTTCGACCCGATCCTCAGTTTGATGCTATCAATATTGTATTGCTTGCTATTCAGAATGATTGTGACTCCATCGTTGAAGTTCATGTACTTATGCGCAGCGATGCTGAAAATTGTCAGAGGAGTCATGACGGGATTTCTGACTGCAAGCTGTTTGTGTTTTATGAAGAGAAGTACTTATTTCATCATTTTATCAAGTTTGTATGCTCGTTGGACCCTGATATATTAATGGGTTGGGATATTCAAGGTGGTTCCCTTGGTTTTTTGGCTGAAAGGGCTTCACATCTTGGTATAGGTTTACTCAACAAGATATCTAGAACACCATCTGAAGCCAAGATGGTGGCTGAGGATTCAGAAGTTCCGGAGAAAGTCTTACAAGATAACCTGGCTGCTGACTCAATCGTTGCTGATTCAGTTGTATTAGAAGATCCAATAATTGAGGATGAATGGGGACGGACTCATTCCAGCGGTGTCCATGTTGGTGGTAGAATTGTACTTAATGTCTGGCGGCTGATGCGCAGTGAAGTGAAGCTTAATATGTACACAGTTGAAGCTGTTTCTGAAGTTGTGTTGAGGCGGAAAGTCCCATATATTCCTAATAAACTACTCACAAAATGGTTTTTAAGTGGTCCTGGAAGAGCAAGATACCGATGTATCGAATATGTGAAGGAGAGAGCAGAATTAAACCTTGAGATGATGAATCAATTAGATATGATAAATCGAACATCAGAACTTGCACGTGTCTTTGGCATTGACTTTTTTTCTGTTCTCTCTAGAGGCTCCCAATACCGTGTTGAATCTATGGTTCTGAGACTGGCACATACCCAAAACTATCTTGCCATTTCCCCTGGGAAACAACAGGTAGCTTCGCAGCCAGCAATGGAATGCATCCCTCTTGTAATGGAACCAGAATCTGGTTTTTATGCAGATCCAGTAGTTGTTTTGGATTTTCAGTCTCTTTATCCATCAATGATAATTGCATACAACCTTTGCTATTCTACATGCCTTGGAAAAGTTGCGCCATCAGAGCCAAATACACTTGGTGTCAGTTCATTTTCACCAGATCCACATGATCTGCATGATTTAAAAGATCAAATACTGCTTACTCCAAATGGTGTTATGTGTGTGCCAGCAAAGGTCCGTAAAGGCGTACTGCCACGCTTATTGGACGAAATATTGTCAACCAGAATAATGGTTAAACAAGCAATGAAAAAATTGTCTCCATCACAGAAAGTTCTTCACAGGATATTTAATGCAAGACAGCTTGCACTAAAGCTGATAGCAAACGTGACATATGGTTATACAGCTGCTGGATTTAGTGGCCGCATGCCTTGTGCAGAGATTGCAGACAGTATTGTTCAGTGTGGCCGTAGCACACTTGAAAAGGCTATTTCATATGTAAATGCACATGACAGGTGGAAAGCTAGAGTTATTTATGGCGACACAGACAGCATGTTTGTCCTTCTAAAAGGCCGCACTAGGGAAGAGTCTTTTCAGATTGGACAGGAGATTGCATCTGAAATCAGTGCTATGAGTCCGACTCCAGTTGCTTTGAAGATGGAAAAAGTTTATCAGCCATGCTTCCTCCTTACCAAGAAGCGATATGTTGGTTACAGTTATGAAAGCCCTGGACAGAGTGAACCTATTTTTGATGCTAAAGGCATTGAGACAGTTCGCAGGGATACATGTGGGGCTGTTGCCAAGACAATGGAGCAGTCTCTGAGACTTTTTTTTGAACATCAAGACATGCCTGAGGTACAGGTGCATGCGTTAAGGCTCTTTCTAAGGGTAAATGCTTCATTTACTAGTTTGGGTGATCACCTATGTTTTTTGCACAGGTCAAAGCATACTTACAGCGCCAGTGGAAGCGAATTCTTTCTGGAAGAGTGTCCCTTCAGGATTTTGTATTTGCAAAAGAGGTTCGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000731 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010225 GO:0016035 GO:0018130 GO:0019438 GO:0019985 GO:0032991 GO:0033554 GO:0034641 GO:0034644 GO:0034645 GO:0034654 GO:0042276 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0071214 GO:0071478 GO:0071482 GO:0071494 GO:0071704 GO:0071897 GO:0090304 GO:0104004 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko01100 ko01524 ko03460 map01100 map01524 map03460

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 RVT_2 zf-C4pol zf-RanBP

Protein Analysis

1757

Amino Acids

194.85

Weight (kDa)

5.57

Isoelectric Point (pI)

49.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
REV3_N	PF24065	6 - 60	2.3e-18	DNA polymerase zeta catalytic subunit, N-terminal
POL3_N	PF24055	62 - 141	5e-22	DNA polymerase delta catalytic subunit-like, N-terminal domain
DNA_pol_B_exo1	PF03104	1100 - 1226	2.4e-08	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	1368 - 1704	1.6e-85	DNA polymerase family B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004063)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500 AT1G67500
fragaria_vesca	FvH4_6g06120 FvH4_6g06120 FvH4_6g06120 FvH4_6g06120 FvH4_6g06120
malus_domestica	MD05G1053400.v1.1 MD05G1053600.v1.1 MD05G1053700.v1.1 MD12G1217900.v1.1
prunus_persica	Prupe.6G324000_v2.0.a1 Prupe.6G324000_v2.0.a1 Prupe.6G324000_v2.0.a1
pyrus_communis	pycom05g04420 pycom12g20340
rosa_chinensis	RchiOBHm_Chr3g0455131
rosa_laevigata	RLG00000025385
rosa_multiflora	Rmu_co8200746.1_g000001 Rmu_sc0041669.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424540
rosa_rugosa	Rorug01G0381700 Rorug03G0004000
rosa_samantha	Rh3AG064900 Rh3BG066900 Rh3CG065300 Rh3DG066800
rosa_wichuraiana	Rw3G005080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3173
AarI	CACCTGC	1 cut(s)	2923
AasI	GACNNNNNNGTC	2 cut(s)	627, 2380
Acc16I	TGCGCA	2 cut(s)	3443, 3863
Acc36I	ACCTGC	2 cut(s)	1579, 2923
Acc65I	GGTACC	1 cut(s)	118
AccB1I	GGYRCC	2 cut(s)	55, 118
AccB7I	CCANNNNNTGG	3 cut(s)	1142, 2590, 4259
AccBSI	CCGCTC	1 cut(s)	3198
AccI	GTMKAC	5 cut(s)	42, 659, 735, 872, 2946
AccII	CGCG	1 cut(s)	429
AccIII	TCCGGA	2 cut(s)	27, 3706
AcoI	YGGCCR	5 cut(s)	150, 2338, 3295, 4666, 4711
AcsI	RAATTY	6 cut(s)	132, 1034, 1150, 2186, 3004, 5216
AcvI	CACGTG	1 cut(s)	4085
AcyI	GRCGYC	1 cut(s)	56
AdeI	CACNNNGTG	1 cut(s)	4388
AflIII	ACRYGT	3 cut(s)	322, 4084, 5030
AgeI	ACCGGT	1 cut(s)	706
AhlI	ACTAGT	1 cut(s)	5150
AjiI	CACGTC	1 cut(s)	901
AjnI	CCWGG	9 cut(s)	67, 345, 669, 1587, 1698, 3729, 3976, 4192, 4972
AjuI	GAANNNNNNNTTGG	2 cut(s)	2319, 2351
AloI	GAACNNNNNNTCC	2 cut(s)	1541, 1573
AlwNI	CAGNNNCTG	7 cut(s)	1889, 2390, 2590, 3304, 4259, 4655, 5065
Ama87I	CYCGRG	2 cut(s)	821, 2318
Aor13HI	TCCGGA	2 cut(s)	27, 3706
AoxI	GGCC	7 cut(s)	150, 1779, 2338, 3295, 4666, 4711, 4816
ApoI	RAATTY	6 cut(s)	132, 1034, 1150, 2186, 3004, 5216
ArsI	GACNNNNNNTTYG	6 cut(s)	795, 827, 1462, 1494, 4784, 4816
AseI	ATTAAT	1 cut(s)	3572
AsiGI	ACCGGT	1 cut(s)	706
Asp700I	GAANNNNTTC	4 cut(s)	1377, 2252, 3120, 5220
Asp718I	GGTACC	1 cut(s)	118
AspA2I	CCTAGG	1 cut(s)	599
AspLEI	GCGC	6 cut(s)	58, 431, 3444, 3864, 4366, 5204
AspS9I	GGNCC	7 cut(s)	1028, 1780, 3083, 3224, 3559, 3974, 4483
AsuC2I	CCSGG	2 cut(s)	627, 3278
AsuII	TTCGAA	1 cut(s)	1165
AvaI	CYCGRG	2 cut(s)	821, 2318
AvaII	GGWCC	6 cut(s)	1028, 3083, 3224, 3559, 3974, 4483
AvrII	CCTAGG	1 cut(s)	599
AxyI	CCTNAGG	2 cut(s)	2860, 5097
BaeI	ACNNNNGTAYC	4 cut(s)	3982, 3982, 4015, 4015
BalI	TGGCCA	1 cut(s)	3297
BamHI	GGATCC	1 cut(s)	1834
BanI	GGYRCC	2 cut(s)	55, 118
BanII	GRGCYC	1 cut(s)	1763
BarI	GAAGNNNNNNTAC	2 cut(s)	3417, 3449
BauI	CACGAG	2 cut(s)	959, 2028
BbrPI	CACGTG	1 cut(s)	4085
BbsI	GAAGAC	2 cut(s)	1799, 2411
BbvCI	CCTCAGC	2 cut(s)	2391, 3690
BceAI	ACGGC	4 cut(s)	154, 190, 2325, 4698
BcgI	CGANNNNNNTGC	2 cut(s)	812, 846
BciT130I	CCWGG	9 cut(s)	69, 347, 671, 1589, 1700, 3731, 3978, 4194, 4974
BciVI	GTATCC	3 cut(s)	948, 2518, 5020
BclI	TGATCA	1 cut(s)	5161
BcnI	CCSGG	2 cut(s)	627, 3278
BcuI	ACTAGT	1 cut(s)	5150
BfmI	CTRYAG	4 cut(s)	85, 660, 837, 1296
BfoI	RGCGCY	2 cut(s)	59, 5205
BfuAI	ACCTGC	2 cut(s)	1579, 2923
BfuI	GTATCC	3 cut(s)	948, 2518, 5020
BglII	AGATCT	1 cut(s)	605
BlnI	CCTAGG	1 cut(s)	599
BmcAI	AGTACT	1 cut(s)	3521
Bme18I	GGWCC	6 cut(s)	1028, 3083, 3224, 3559, 3974, 4483
BmeT110I	CYCGRG	2 cut(s)	821, 2318
BmgBI	CACGTC	1 cut(s)	901
BmgT120I	GGNCC	7 cut(s)	1028, 1780, 3083, 3224, 3559, 3974, 4483
BmiI	GGNNCC	7 cut(s)	57, 120, 1836, 3561, 3598, 4126, 4251
BmrI	ACTGGG	3 cut(s)	660, 1611, 2600
BmuI	ACTGGG	3 cut(s)	660, 1611, 2600
BpiI	GAAGAC	2 cut(s)	1799, 2411
BplI	GAGNNNNNCTC	2 cut(s)	4870, 4902
BpmI	CTGGAG	4 cut(s)	1454, 1571, 2247, 4872
Bpu10I	CCTNAGC	2 cut(s)	2391, 3690
Bpu14I	TTCGAA	1 cut(s)	1165
BpuEI	CTTGAG	3 cut(s)	1251, 3072, 4055
BpuMI	CCSGG	2 cut(s)	627, 3278
BsaAI	YACGTR	1 cut(s)	4085
BsaBI	GATNNNNATC	2 cut(s)	1533, 3241
BsaHI	GRCGYC	1 cut(s)	56
BsaI	GGTCTC	1 cut(s)	3267
BsaWI	WCCGGW	3 cut(s)	27, 706, 3706
BsaXI	ACNNNNNCTCC	4 cut(s)	441, 471, 1612, 1642
Bse118I	RCCGGY	2 cut(s)	152, 706
Bse21I	CCTNAGG	2 cut(s)	2860, 5097
Bse3DI	GCAATG	2 cut(s)	4230, 4557
Bse8I	GATNNNNATC	2 cut(s)	1533, 3241
BseAI	TCCGGA	2 cut(s)	27, 3706
BseBI	CCWGG	9 cut(s)	69, 347, 671, 1589, 1700, 3731, 3978, 4194, 4974
BseJI	GATNNNNATC	2 cut(s)	1533, 3241
BseMI	GCAATG	2 cut(s)	4230, 4557
BseRI	GAGGAG	5 cut(s)	395, 950, 1846, 1954, 3481
BseX3I	CGGCCG	1 cut(s)	150
BsgI	GTGCAG	4 cut(s)	155, 474, 778, 4701
Bsh1236I	CGCG	1 cut(s)	429
Bsh1285I	CGRYCG	1 cut(s)	153
BshFI	GGCC	7 cut(s)	152, 1781, 2340, 3297, 4668, 4713, 4818
BshNI	GGYRCC	2 cut(s)	55, 118
BshTI	ACCGGT	1 cut(s)	706
BsiEI	CGRYCG	1 cut(s)	153
BsiHKCI	CYCGRG	2 cut(s)	821, 2318
BsiSI	CCGG	7 cut(s)	28, 149, 153, 627, 707, 3278, 3707
BslFI	GGGAC	5 cut(s)	1943, 2852, 3810, 3914, 5220
BsmBI	CGTCTC	2 cut(s)	40, 1223
BsmFI	GGGAC	5 cut(s)	1943, 2852, 3810, 3914, 5220
BsmI	GAATGC	1 cut(s)	4235
BsnI	GGCC	7 cut(s)	152, 1781, 2340, 3297, 4668, 4713, 4818
Bso31I	GGTCTC	1 cut(s)	3267
BsoBI	CYCGRG	2 cut(s)	821, 2318
Bsp119I	TTCGAA	1 cut(s)	1165
Bsp1286I	GDGCHC	1 cut(s)	1763
Bsp13I	TCCGGA	2 cut(s)	27, 3706
Bsp1407I	TGTACA	3 cut(s)	325, 1818, 3885
BspANI	GGCC	7 cut(s)	152, 1781, 2340, 3297, 4668, 4713, 4818
BspEI	TCCGGA	2 cut(s)	27, 3706
BspFNI	CGCG	1 cut(s)	429
BspHI	TCATGA	2 cut(s)	2077, 3472
BspLI	GGNNCC	7 cut(s)	57, 120, 1836, 3561, 3598, 4126, 4251
BspMAI	CTGCAG	1 cut(s)	1300
BspMI	ACCTGC	2 cut(s)	1579, 2923
BspQI	GCTCTTC	2 cut(s)	2269, 3976
BspT104I	TTCGAA	1 cut(s)	1165
BspT107I	GGYRCC	2 cut(s)	55, 118
BspTNI	GGTCTC	1 cut(s)	3267
BsrBI	CCGCTC	1 cut(s)	3198
BsrDI	GCAATG	2 cut(s)	4230, 4557
BsrFI	RCCGGY	2 cut(s)	152, 706
BsrGI	TGTACA	3 cut(s)	325, 1818, 3885
BssAI	RCCGGY	2 cut(s)	152, 706
BssNAI	GTATAC	2 cut(s)	873, 2947
BssNI	GRCGYC	1 cut(s)	56
BssSI	CACGAG	2 cut(s)	959, 2028
BssT1I	CCWWGG	5 cut(s)	599, 2335, 3079, 3601, 4351
Bst1107I	GTATAC	2 cut(s)	873, 2947
Bst2BI	CACGAG	2 cut(s)	959, 2028
Bst2UI	CCWGG	9 cut(s)	69, 347, 671, 1589, 1700, 3731, 3978, 4194, 4974
Bst6I	CTCTTC	6 cut(s)	1221, 2269, 3507, 3976, 4824, 5223
BstACI	GRCGYC	1 cut(s)	56
BstAPI	GCANNNNNTGC	2 cut(s)	1126, 4688
BstAUI	TGTACA	3 cut(s)	325, 1818, 3885
BstBAI	YACGTR	1 cut(s)	4085
BstBI	TTCGAA	1 cut(s)	1165
BstDSI	CCRYGG	1 cut(s)	97
BstEII	GGTNACC	1 cut(s)	365
BstENI	CCTNNNNNAGG	2 cut(s)	344, 2680
BstFNI	CGCG	1 cut(s)	429
BstH2I	RGCGCY	2 cut(s)	59, 5205
BstHHI	GCGC	6 cut(s)	58, 431, 3444, 3864, 4366, 5204
BstMCI	CGRYCG	1 cut(s)	153
BstNI	CCWGG	9 cut(s)	69, 347, 671, 1589, 1700, 3731, 3978, 4194, 4974
BstNSI	RCATGY	9 cut(s)	326, 1578, 1883, 4350, 4675, 4801, 5034, 5095, 5114
BstPI	GGTNACC	1 cut(s)	365
BstSFI	CTRYAG	4 cut(s)	85, 660, 837, 1296
BstUI	CGCG	1 cut(s)	429
BstV2I	GAAGAC	2 cut(s)	1799, 2411
BstX2I	RGATCY	7 cut(s)	605, 1199, 1834, 2975, 3772, 4273, 4408
BstXI	CCANNNNNNTGG	6 cut(s)	738, 1595, 3685, 3827, 4537, 5053
BstYI	RGATCY	7 cut(s)	605, 1199, 1834, 2975, 3772, 4273, 4408
BstZ17I	GTATAC	2 cut(s)	873, 2947
BstZI	CGGCCG	1 cut(s)	150
Bsu36I	CCTNAGG	2 cut(s)	2860, 5097
BsuI	GTATCC	3 cut(s)	948, 2518, 5020
BsuRI	GGCC	7 cut(s)	152, 1781, 2340, 3297, 4668, 4713, 4818
BtgI	CCRYGG	1 cut(s)	97
BtgZI	GCGATG	1 cut(s)	3462
BtrI	CACGTC	1 cut(s)	901
BtsI	GCAGTG	4 cut(s)	307, 462, 2155, 3871
BveI	ACCTGC	2 cut(s)	1579, 2923
CaiI	CAGNNNCTG	7 cut(s)	1889, 2390, 2590, 3304, 4259, 4655, 5065
CciI	TCATGA	2 cut(s)	2077, 3472
CfoI	GCGC	6 cut(s)	58, 431, 3444, 3864, 4366, 5204
Cfr10I	RCCGGY	2 cut(s)	152, 706
Cfr13I	GGNCC	7 cut(s)	1028, 1780, 3083, 3224, 3559, 3974, 4483
CseI	GACGC	1 cut(s)	13
CspAI	ACCGGT	1 cut(s)	706
CspCI	CAANNNNNGTGG	2 cut(s)	4491, 4526
DinI	GGCGCC	1 cut(s)	57
DraI	TTTAAA	3 cut(s)	2163, 2562, 4431
DraIII	CACNNNGTG	1 cut(s)	4388
DrdI	GACNNNNNNGTC	2 cut(s)	627, 2380
DseDI	GACNNNNNNGTC	2 cut(s)	627, 2380
EaeI	YGGCCR	5 cut(s)	150, 2338, 3295, 4666, 4711
EagI	CGGCCG	1 cut(s)	150
Eam1104I	CTCTTC	6 cut(s)	1221, 2269, 3507, 3976, 4824, 5223
EarI	CTCTTC	6 cut(s)	1221, 2269, 3507, 3976, 4824, 5223
EciI	GGCGGA	1 cut(s)	3937
EclXI	CGGCCG	1 cut(s)	150
Eco130I	CCWWGG	5 cut(s)	599, 2335, 3079, 3601, 4351
Eco24I	GRGCYC	1 cut(s)	1763
Eco31I	GGTCTC	1 cut(s)	3267
Eco32I	GATATC	3 cut(s)	79, 717, 3657
Eco47I	GGWCC	6 cut(s)	1028, 3083, 3224, 3559, 3974, 4483
Eco52I	CGGCCG	1 cut(s)	150
Eco72I	CACGTG	1 cut(s)	4085
Eco81I	CCTNAGG	2 cut(s)	2860, 5097
Eco88I	CYCGRG	2 cut(s)	821, 2318
Eco91I	GGTNACC	1 cut(s)	365
EcoNI	CCTNNNNNAGG	2 cut(s)	344, 2680
EcoO109I	RGGNCCY	1 cut(s)	3083
EcoO65I	GGTNACC	1 cut(s)	365
EcoRI	GAATTC	2 cut(s)	3004, 5216
EcoRII	CCWGG	9 cut(s)	67, 345, 669, 1587, 1698, 3729, 3976, 4192, 4972
EcoRV	GATATC	3 cut(s)	79, 717, 3657
EcoT14I	CCWWGG	5 cut(s)	599, 2335, 3079, 3601, 4351
EcoT22I	ATGCAT	1 cut(s)	4235
EcoT38I	GRGCYC	1 cut(s)	1763
EgeI	GGCGCC	1 cut(s)	57
EheI	GGCGCC	1 cut(s)	57
ErhI	CCWWGG	5 cut(s)	599, 2335, 3079, 3601, 4351
Esp3I	CGTCTC	2 cut(s)	40, 1223
FalI	AAGNNNNNCTT	8 cut(s)	1971, 2003, 2319, 2351, 3109, 3141, 3861, 3893
FaqI	GGGAC	5 cut(s)	1943, 2852, 3810, 3914, 5220
FauI	CCCGC	1 cut(s)	1920
FauNDI	CATATG	3 cut(s)	256, 4639, 4741
FbaI	TGATCA	1 cut(s)	5161
FblI	GTMKAC	5 cut(s)	42, 659, 735, 872, 2946
FriOI	GRGCYC	1 cut(s)	1763
FspI	TGCGCA	2 cut(s)	3443, 3863
GlaI	GCGC	6 cut(s)	57, 430, 3443, 3863, 4365, 5203
GsuI	CTGGAG	4 cut(s)	1454, 1571, 2247, 4872
HaeII	RGCGCY	2 cut(s)	59, 5205
HaeIII	GGCC	7 cut(s)	152, 1781, 2340, 3297, 4668, 4713, 4818
HapII	CCGG	7 cut(s)	28, 149, 153, 627, 707, 3278, 3707
HgaI	GACGC	1 cut(s)	13
HhaI	GCGC	6 cut(s)	58, 431, 3444, 3864, 4366, 5204
Hin1I	GRCGYC	1 cut(s)	56
Hin6I	GCGC	6 cut(s)	56, 429, 3442, 3862, 4364, 5202
HinP1I	GCGC	6 cut(s)	56, 429, 3442, 3862, 4364, 5202
HincII	GTYRAC	5 cut(s)	43, 1209, 2155, 2910, 4527
HindII	GTYRAC	5 cut(s)	43, 1209, 2155, 2910, 4527
HindIII	AAGCTT	5 cut(s)	162, 282, 356, 2328, 3875
HpaI	GTTAAC	1 cut(s)	2910
HpaII	CCGG	7 cut(s)	28, 149, 153, 627, 707, 3278, 3707
Hpy99I	CGWCG	1 cut(s)	44
HpyCH4IV	ACGT	6 cut(s)	900, 1907, 2174, 3308, 4084, 4632
HpySE526I	ACGT	6 cut(s)	900, 1907, 2174, 3308, 4084, 4632
Hsp92I	GRCGYC	1 cut(s)	56
HspAI	GCGC	6 cut(s)	56, 429, 3442, 3862, 4364, 5202
KasI	GGCGCC	1 cut(s)	55
Kpn2I	TCCGGA	2 cut(s)	27, 3706
KpnI	GGTACC	1 cut(s)	122
Ksp22I	TGATCA	1 cut(s)	5161
KspAI	GTTAAC	1 cut(s)	2910
LguI	GCTCTTC	2 cut(s)	2269, 3976
LmnI	GCTCC	6 cut(s)	1727, 2228, 2278, 2750, 4130, 5056
MaeII	ACGT	6 cut(s)	900, 1907, 2174, 3308, 4084, 4632
MbiI	CCGCTC	1 cut(s)	3198
MfeI	CAATTG	2 cut(s)	2232, 2694
MflI	RGATCY	7 cut(s)	605, 1199, 1834, 2975, 3772, 4273, 4408
MhlI	GDGCHC	1 cut(s)	1763
MlsI	TGGCCA	1 cut(s)	3297
MluNI	TGGCCA	1 cut(s)	3297
Mly113I	GGCGCC	1 cut(s)	56
MmeI	TCCRAC	4 cut(s)	36, 1138, 3537, 4907
Mox20I	TGGCCA	1 cut(s)	3297
Mph1103I	ATGCAT	1 cut(s)	4235
MroI	TCCGGA	2 cut(s)	27, 3706
MroXI	GAANNNNTTC	4 cut(s)	1377, 2252, 3120, 5220
MscI	TGGCCA	1 cut(s)	3297
MslI	CAYNNNNRTG	2 cut(s)	327, 3825
Msp20I	TGGCCA	1 cut(s)	3297
MspA1I	CMGCKG	5 cut(s)	1349, 2952, 3304, 3813, 4652
MspI	CCGG	7 cut(s)	28, 149, 153, 627, 707, 3278, 3707
MunI	CAATTG	2 cut(s)	2232, 2694
Mva1269I	GAATGC	1 cut(s)	4235
MvaI	CCWGG	9 cut(s)	69, 347, 671, 1589, 1700, 3731, 3978, 4194, 4974
MvnI	CGCG	1 cut(s)	429
NarI	GGCGCC	1 cut(s)	56
NciI	CCSGG	2 cut(s)	627, 3278
NdeI	CATATG	3 cut(s)	256, 4639, 4741
NlaIV	GGNNCC	7 cut(s)	57, 120, 1836, 3561, 3598, 4126, 4251
NmuCI	GTSAC	6 cut(s)	421, 683, 2462, 2539, 3410, 4633
NsbI	TGCGCA	2 cut(s)	3443, 3863
NsiI	ATGCAT	1 cut(s)	4235
NspI	RCATGY	9 cut(s)	326, 1578, 1883, 4350, 4675, 4801, 5034, 5095, 5114
NspV	TTCGAA	1 cut(s)	1165
PaeI	GCATGC	2 cut(s)	4675, 5114
PaeR7I	CTCGAG	2 cut(s)	821, 2318
PagI	TCATGA	2 cut(s)	2077, 3472
PaqCI	CACCTGC	1 cut(s)	2923
PasI	CCCWGGG	1 cut(s)	4193
PciI	ACATGT	2 cut(s)	322, 5030
PciSI	GCTCTTC	2 cut(s)	2269, 3976
PcsI	WCGNNNNNNNCGW	1 cut(s)	39
PctI	GAATGC	1 cut(s)	4235
PdmI	GAANNNNTTC	4 cut(s)	1377, 2252, 3120, 5220
PflMI	CCANNNNNTGG	3 cut(s)	1142, 2590, 4259
PfoI	TCCNGGA	1 cut(s)	3976
PinAI	ACCGGT	1 cut(s)	706
PluTI	GGCGCC	1 cut(s)	59
PmaCI	CACGTG	1 cut(s)	4085
PmlI	CACGTG	1 cut(s)	4085
Ppu21I	YACGTR	1 cut(s)	4085
PpuMI	RGGWCCY	1 cut(s)	3083
PscI	ACATGT	2 cut(s)	322, 5030
PshBI	ATTAAT	1 cut(s)	3572
PsiI	TTATAA	1 cut(s)	3173
Psp5II	RGGWCCY	1 cut(s)	3083
Psp6I	CCWGG	9 cut(s)	67, 345, 669, 1587, 1698, 3729, 3976, 4192, 4972
PspCI	CACGTG	1 cut(s)	4085
PspEI	GGTNACC	1 cut(s)	365
PspGI	CCWGG	9 cut(s)	67, 345, 669, 1587, 1698, 3729, 3976, 4192, 4972
PspN4I	GGNNCC	7 cut(s)	57, 120, 1836, 3561, 3598, 4126, 4251
PspPI	GGNCC	7 cut(s)	1028, 1780, 3083, 3224, 3559, 3974, 4483
PspPPI	RGGWCCY	1 cut(s)	3083
PspXI	VCTCGAGB	2 cut(s)	821, 2318
PstI	CTGCAG	1 cut(s)	1300
PstNI	CAGNNNCTG	7 cut(s)	1889, 2390, 2590, 3304, 4259, 4655, 5065
PsuI	RGATCY	7 cut(s)	605, 1199, 1834, 2975, 3772, 4273, 4408
PvuII	CAGCTG	3 cut(s)	1349, 3304, 4652
RseI	CAYNNNNRTG	2 cut(s)	327, 3825
SalI	GTCGAC	1 cut(s)	41
SapI	GCTCTTC	2 cut(s)	2269, 3976
Sau96I	GGNCC	7 cut(s)	1028, 1780, 3083, 3224, 3559, 3974, 4483
ScaI	AGTACT	1 cut(s)	3521
SduI	GDGCHC	1 cut(s)	1763
SfcI	CTRYAG	4 cut(s)	85, 660, 837, 1296
SfoI	GGCGCC	1 cut(s)	57
Sfr274I	CTCGAG	2 cut(s)	821, 2318
SfuI	TTCGAA	1 cut(s)	1165
SinI	GGWCC	6 cut(s)	1028, 3083, 3224, 3559, 3974, 4483
SlaI	CTCGAG	2 cut(s)	821, 2318
SmiMI	CAYNNNNRTG	2 cut(s)	327, 3825
SmlI	CTYRAG	5 cut(s)	821, 1266, 2318, 3087, 4034
SmoI	CTYRAG	5 cut(s)	821, 1266, 2318, 3087, 4034
SpeI	ACTAGT	1 cut(s)	5150
SphI	GCATGC	2 cut(s)	4675, 5114
SspDI	GGCGCC	1 cut(s)	55
SspI	AATATT	3 cut(s)	1654, 3382, 4521
StyI	CCWWGG	5 cut(s)	599, 2335, 3079, 3601, 4351
TaiI	ACGT	6 cut(s)	903, 1910, 2177, 3311, 4087, 4635
TatI	WGTACW	6 cut(s)	325, 1818, 3433, 3519, 3838, 3885
TauI	GCSGC	7 cut(s)	7, 61, 434, 1781, 3856, 4671, 4821
TseFI	GTSAC	6 cut(s)	421, 683, 2462, 2539, 3410, 4633
Tsp45I	GTSAC	6 cut(s)	421, 683, 2462, 2539, 3410, 4633
TspGWI	ACGGA	4 cut(s)	28, 1247, 3814, 4475
Van91I	CCANNNNNTGG	3 cut(s)	1142, 2590, 4259
VpaK11BI	GGWCC	6 cut(s)	1028, 3083, 3224, 3559, 3974, 4483
VspI	ATTAAT	1 cut(s)	3572
XagI	CCTNNNNNAGG	2 cut(s)	344, 2680
XapI	RAATTY	6 cut(s)	132, 1034, 1150, 2186, 3004, 5216
XbaI	TCTAGA	3 cut(s)	3338, 3659, 4118
XceI	RCATGY	9 cut(s)	326, 1578, 1883, 4350, 4675, 4801, 5034, 5095, 5114
XcmI	CCANNNNNNNNNTGG	3 cut(s)	442, 695, 2700
XhoI	CTCGAG	2 cut(s)	821, 2318
XmaJI	CCTAGG	1 cut(s)	599
XmiI	GTMKAC	5 cut(s)	42, 659, 735, 872, 2946
XmnI	GAANNNNTTC	4 cut(s)	1377, 2252, 3120, 5220
ZrmI	AGTACT	1 cut(s)	3521
Zsp2I	ATGCAT	1 cut(s)	4235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.